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DOE OSTI · 3367534

High-density Lipoprotein (HDL) Structure and Function Proteomics (JM-DP1)

Sarkar, Snigdha·Melchior, John T.·Sims, Amy
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Abstract

The purpose of this experiment was to investigate how the interactions between APOA1 and APOA2 on the surface of high-density lipoproteins (HDL) impact particle function by studying the effect of exogenous APOA2 on HDL structure through limited proteolysis. Interactions were investigated on HDL isolated from human blood plasma using structural proteomics tools such as chemical cross-linking and limited proteolysis (LiP). The structural proteomics data was acquired using a Q-Exactive HF-X mass spectrometer and processed using MaxQuant software (v.1.6.17.0).

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59 BASIC BIOLOGICAL SCIENCESApolipoprotein A-IApolipoprotein A-II ABCA1Apolipoproteinscholesterol effluxHigh Density LipoproteinsLimited Proteolysis ProteomicsLipoprotein metabolismLipoproteins Structure

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Sarkar, Snigdha, Melchior, John T., Sims, Amy. 2024-09-24. High-density Lipoprotein (HDL) Structure and Function Proteomics (JM-DP1). https://doi.org/10.25584/3367534

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