Demonstrating a butylamine-based deconstruction method for poplar biomass and conversion by diverse microbial strains

Jia, Jiayuan [Joint BioEnergy Institute (JBEI), Emeryville, CA (United States); Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)]; Dietrich, Demian [Joint BioEnergy Institute (JBEI), Emeryville, CA (United States); Sandia National Laboratories (SNL-CA), Livermore, CA (United States)] (ORCID:000000032890109X); Pidatala, Venkataramana R. [Joint BioEnergy Institute (JBEI), Emeryville, CA (United States); Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)]; Turumtay, Emine Akyuz [Joint BioEnergy Institute (JBEI), Emeryville, CA (United States); Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)]; Baidoo, Edward E. K. [Joint BioEnergy Institute (JBEI), Emeryville, CA (United States); Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)]; Barcelos, Carolina A. [Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States); Lawrence Berkeley National Laboratory (LBNL), Emeryville, CA (United States). Advanced BioProcess Development Unit (ABPDU)]; Palasz, Joseph [Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)]; Rahman, Md Maksudur [Joint BioEnergy Institute (JBEI), Emeryville, CA (United States); Sandia National Laboratories (SNL-CA), Livermore, CA (United States)]; Kang, Chae Won [Joint BioEnergy Institute (JBEI), Emeryville, CA (United States); Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)]; Garcia, Valentina E. [Joint BioEnergy Institute (JBEI), Emeryville, CA (United States); Sandia National Laboratories (SNL-CA), Livermore, CA (United States)] (ORCID:0000000259360882); Koleski, Edward [Joint BioEnergy Institute (JBEI), Emeryville, CA (United States); Lawrence Berkeley National Laboratory (LBNL), Emeryville, CA (United States). Advanced BioProcess Development Unit (ABPDU)]; Panich, Justin [Joint BioEnergy Institute (JBEI), Emeryville, CA (United States); Lawrence Berkeley National Laboratory (LBNL), Emeryville, CA (United States). Advanced BioProcess Development Unit (ABPDU)]; Sundstrom, Eric R. [Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States); Lawrence Berkeley National Laboratory (LBNL), Emeryville, CA (United States). Advanced BioProcess Development Unit (ABPDU)] (ORCID:0000000349835415); Eudes, Aymerick [Joint BioEnergy Institute (JBEI), Emeryville, CA (United States); Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)] (ORCID:0000000213876111); Choudhary, Hemant [Joint BioEnergy Institute (JBEI), Emeryville, CA (United States); Sandia National Laboratories (SNL-CA), Livermore, CA (United States)] (ORCID:0000000328473080); Lee, Taek Soon [Joint BioEnergy Institute (JBEI), Emeryville, CA (United States); Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)]; Gladden, John M. [Joint BioEnergy Institute (JBEI), Emeryville, CA (United States); Sandia National Laboratories (SNL-CA), Livermore, CA (United States)] (ORCID:0000000269852485); Simmons, Blake A. [Joint BioEnergy Institute (JBEI), Emeryville, CA (United States); Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)] (ORCID:0000000213321810); Kim, Joonhoon [Joint BioEnergy Institute (JBEI), Emeryville, CA (United States); Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)] (ORCID:0000000274251828); Rodriguez, Alberto [Joint BioEnergy Institute (JBEI), Emeryville, CA (United States); Sandia National Laboratories (SNL-CA), Livermore, CA (United States)] (ORCID:0000000240450108)

doi:10.1039/d5gc06335c

DOE OSTI · 3389467

Demonstrating a butylamine-based deconstruction method for poplar biomass and conversion by diverse microbial strains

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Abstract

Low-boiling alkylamines such as butylamine offer promise as effective biomass pretreatment solvents that can be readily recovered and recycled; however, their capability to support microbial conversion of nutrients present in hydrolysates represents an important area for investigation. Here we employed butylamine to pretreat poplar biomass and characterize its effects on the release of fermentable sugars after solvent removal and enzymatic hydrolysis, as well as the biocompatibility of the produced hydrolysates with three organisms commonly used as bioconversion hosts. We observed that residual butylamine and the derivative butylacetamide were present in high enough concentrations to exert toxicity to strains of Aspergillus niger, Pseudomonas putida, and Rhodosporidium toruloides that produce malic acid, isoprenol and bisabolene, respectively. Removal of the toxic compounds by charcoal filtration and nutrient supplementation resulted in a hydrolysate containing >100 g L −1 of sugars that enabled strong growth, substrate consumption and bioproduct accumulation, outperforming defined cultivation media. This is the first demonstration of a butylamine-based deconstruction process for poplar biomass at a pilot-scale to achieve conversion of high sugar concentrations to valuable bioproducts with engineered microbes.

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Jia, Jiayuan [Joint BioEnergy Institute (JBEI), Emeryville, CA (United States); Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)], Dietrich, Demian [Joint BioEnergy Institute (JBEI), Emeryville, CA (United States); Sandia National Laboratories (SNL-CA), Livermore, CA (United States)] (ORCID:000000032890109X), Pidatala, Venkataramana R. [Joint BioEnergy Institute (JBEI), Emeryville, CA (United States); Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)], Turumtay, Emine Akyuz [Joint BioEnergy Institute (JBEI), Emeryville, CA (United States); Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)], Baidoo, Edward E. K. [Joint BioEnergy Institute (JBEI), Emeryville, CA (United States); Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)], Barcelos, Carolina A. [Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States); Lawrence Berkeley National Laboratory (LBNL), Emeryville, CA (United States). Advanced BioProcess Development Unit (ABPDU)], Palasz, Joseph [Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)], Rahman, Md Maksudur [Joint BioEnergy Institute (JBEI), Emeryville, CA (United States); Sandia National Laboratories (SNL-CA), Livermore, CA (United States)], Kang, Chae Won [Joint BioEnergy Institute (JBEI), Emeryville, CA (United States); Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)], Garcia, Valentina E. [Joint BioEnergy Institute (JBEI), Emeryville, CA (United States); Sandia National Laboratories (SNL-CA), Livermore, CA (United States)] (ORCID:0000000259360882), Koleski, Edward [Joint BioEnergy Institute (JBEI), Emeryville, CA (United States); Lawrence Berkeley National Laboratory (LBNL), Emeryville, CA (United States). Advanced BioProcess Development Unit (ABPDU)], Panich, Justin [Joint BioEnergy Institute (JBEI), Emeryville, CA (United States); Lawrence Berkeley National Laboratory (LBNL), Emeryville, CA (United States). Advanced BioProcess Development Unit (ABPDU)], Sundstrom, Eric R. [Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States); Lawrence Berkeley National Laboratory (LBNL), Emeryville, CA (United States). Advanced BioProcess Development Unit (ABPDU)] (ORCID:0000000349835415), Eudes, Aymerick [Joint BioEnergy Institute (JBEI), Emeryville, CA (United States); Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)] (ORCID:0000000213876111), Choudhary, Hemant [Joint BioEnergy Institute (JBEI), Emeryville, CA (United States); Sandia National Laboratories (SNL-CA), Livermore, CA (United States)] (ORCID:0000000328473080), Lee, Taek Soon [Joint BioEnergy Institute (JBEI), Emeryville, CA (United States); Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)], Gladden, John M. [Joint BioEnergy Institute (JBEI), Emeryville, CA (United States); Sandia National Laboratories (SNL-CA), Livermore, CA (United States)] (ORCID:0000000269852485), Simmons, Blake A. [Joint BioEnergy Institute (JBEI), Emeryville, CA (United States); Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)] (ORCID:0000000213321810), Kim, Joonhoon [Joint BioEnergy Institute (JBEI), Emeryville, CA (United States); Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)] (ORCID:0000000274251828), Rodriguez, Alberto [Joint BioEnergy Institute (JBEI), Emeryville, CA (United States); Sandia National Laboratories (SNL-CA), Livermore, CA (United States)] (ORCID:0000000240450108). 2026-05-15. Demonstrating a butylamine-based deconstruction method for poplar biomass and conversion by diverse microbial strains. https://doi.org/10.1039/d5gc06335c

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