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At least 181 records · Page 10

Chronic Hypergravity Induces Changes in the Dopaminergic Neuronal System in Drosophila Melanogaster

Upon atmospheric exitre-entry and during training, astronauts are subjected to temporary periods of hypergravity, which has been implicated in the activation of oxidative stress pathways contributing to mitochondrial dysfunction and neuronal degeneration. The pathogenesis of Parkinsons disease and other neurodegenerative disorders is associated with oxidative damage to neurons involved in dopamine systems of the brain. Our study aims to examine the effects of a hypergravitational developmental environment on the degeneration of dopaminergic systems in Drosophila melanogaster. Male and female flies (Gal4-UAS transgenic line) were hatched and raised to adulthood in centrifugal hypergravity (97rpm, 3g). The nuclear expression of the reporter, Green Fluorescent Protein (GFP) is driven by the dopaminergic enzyme tyrosine hydroxylase (TH) promoter, allowing for the targeted visualization of dopamine producing neurons. After being raised to adulthood and kept in hypergravity until 18 days of age, flies were dissected and the expression of TH was measured by fluorescence confocal microscopy. TH expression in the fly brains was used to obtain counts of healthy dopaminergic neurons for flies raised in chronic hypergravity and control groups. Dopaminergic neuron expression data were compared with those of previous studies that limited hypergravity exposure to late life in order to determine the flies adaptability to the gravitational environment when raised from hatching through adulthood. Overall, we observed a significant effect of chronic hypergravity exposure contributing to deficits in dopaminergic neuron expression (p 0.003). Flies raised in 3g had on average lower dopaminergic neuron counts (mean 97.7) when compared with flies raised in 1g (mean 122.8). We suspect these lower levels of TH expression are a result of oxidative dopaminergic cell loss in flies raised in hypergravity. In future studies, we hope to further elucidate the mechanism by which hypergravity-induced oxidative stress damages the dopaminergic neuronal system, as well as examining possible chemical countermeasures to the hypergravity-induced oxidative stress response in dopaminergic neurons in order to combat cell death and consequent mental and behavioral deficits.

Parkinson's Disorder↗

Effects of Chronic Hypergravity on the Dopaminergic Neuronal System in Drosophila Melanogaster

Upon atmospheric exitre-entry and during training, astronauts are subjected to temporary periods of hypergravity, which has been implicated in the activation of oxidative stress pathways contributing to mitochondrial dysfunction and neuronal degeneration. The pathogenesis of Parkinsons disease and other neurodegenerative disorders is associated with oxidative damage to neurons involved in dopamine systems of the brain. Our study aims to examine the effects of a hypergravitational developmental environment on the degeneration of dopaminergic systems in Drosophila melanogaster. Male and female flies (Gal4-UAS transgenic line) were hatched and raised to adulthood in centrifugal hypergravity (97rpm, 3g). The nuclear expression of the reporter, Green Fluorescent Protein (GFP) is driven by the dopaminergic enzyme tyrosine hydroxylase (TH) promoter, allowing for the targeted visualization of dopamine producing neurons. After being raised to adulthood and kept in hypergravity until 18 days of age, flies were dissected and the expression of TH was measured by fluorescence confocal microscopy. TH expression in the fly brains was used to obtain counts of healthy dopaminergic neurons for flies raised in chronic hypergravity and control groups. Dopaminergic neuron expression data were compared with those of previous studies that limited hypergravity exposure to late life in order to determine the flies adaptability to the gravitational environment when raised from hatching through adulthood. Overall, we observed a significant effect of chronic hypergravity exposure contributing to deficits in dopaminergic neuron expression (p 0.003). Flies raised in 3g had on average lower dopaminergic neuron counts (mean 97.7) when compared with flies raised in 1g (mean 122.8). We suspect these lower levels of TH expression are a result of oxidative dopaminergic cell loss in flies raised in hypergravity. In future studies, we hope to further elucidate the mechanism by which hypergravity-induced oxidative stress damages the dopaminergic neuronal system, as well as examining possible chemical countermeasures to the hypergravity-induced oxidative stress response in dopaminergic neurons in order to combat cell death and consequent mental and behavioral deficits.

Pelos, Andrew↗

Results of a Manned Over Pressurization Event in the Extravehicular Mobility Unit Space Suit Assembly

The Neutral Buoyancy Laboratory (NBL) is a 102 x 202 x 40-foot-deep tank holding over 6 million gallons of water used to simulate weightlessness for Astronaut training. The maxim "Train Like You Fly" refers to the desire to have the suit perform, during training, as close as possible to how it performs during an Extra-Vehicular Activity (EVA), particularly with respect to mobility. Therefore, the Space Suit Assembly (SSA) used in the NBL is a downgraded hardware version of the flight SSA; it is not designed for the NBL environment or operations. A classification system defines the flight Space Suit Assembly hardware as Class I, and the NBL training hardware SSA as Class IIIW. On July 20, 2017, during a manned training event in the NBL, the SSA was inadvertently over-pressurized to 22 psid; normal operating pressure being 4.3 psid. The suit subject was removed from the suit with no injury. The event was investigated by a NASA Mishap Team. The Team investigated common causes and differences between the Class I and Class IIIW Extra-vehicular Mobility Unit (EMU). The investigation determined that the event was limited to Class IIIW hardware and its external flow-controlled open loop ventilation systems. The flight EMU is a pressure regulated closed loop ventilation system. This paper will examine the differences between the Class I and Class IIIW SSA hardware and provide details of the Mishap Investigation. Corrective actions taken to mitigate risk with hardware, operations, and hazard documentation will be discussed.

Spacesuit↗

Internship in Augmented and Virtual Reality - Rapid Model Import Tool

The integration of virtual and augmented reality, sometimes called mixed reality, is an emerging technology which will likely skyrocket overnight much in the way smartphones did a decade ago. Kennedy Space Center's Augmented and Virtual Reality (AVR) Lab is developing a Rapid Model Import Tool (RMIT) to create a quick and efficient way to bring NASA's complex engineering 3D models into virtual and augmented environments. The long-term objective is to create a tool that will ultimately benefit KSC engineers. Its various uses within NASA can potentially span from astronaut training, to marketing, to public outreach, to name a few. Unity is a prolific cross-platform game engine that allows users to build high quality 2D and 3D games for desktop, mobile, web, and game console platforms. It is perhaps also the most widely used software for virtual reality game development. At the AVR lab, we are looking at alternative uses of Unity to build tools for NASA engineers to perform design, development, testing, and training on spacecraft, rocket delivery systems, ground support equipment, and facilities at KSC. As an intern for the RMIT project, I am charged with the task of performing research on Unity-compatible file types to develop an efficient, affordable, preservative process to bring models from CATIA 3D engineering software into the Unity environment. With a tool called the NASA Enterprise Visualization Application (NEVA), developed by the Boeing Design Visualization group at KSC, we are able to easily convert CATIA's design models to. DAE (also known as COLLADA) and .OBJ file formats. I first reduce the polygon count of the model within CATIA itself, make any necessary tweaks to reduce the model further, and then export using NEVA. The .OBJ or. DAE files that I am left with are then converted by another intern to a Unity-compatible file format using a custom Python script. I have generated extensive documentation of this process in a NEVA User Guide. By the end of this semester, we will have built a solid framework for RMIT based on a thorough understanding of virtual reality specifications and file requirements, allowing future software development teams to go forward with development on the custom tool.

Leap Motion↗

Earth Science and Remote Sensing Data Portal Earth Science and Remote Sensing Geospatial Data Portal

The Earth Science and Remote Sensing (ESRS) Data Portal, managed by the ESRS Unit at NASA's Johnson Space Center, is a web GIS environment built on Esri's ArcGIS Enterprise platform. Its current intent is to visualize, analyze, and distribute geospatial data to our internal organization and the communities of Houston and Galveston. Applications on the ESRS Data Portal include a Regional Remote Sensing web app, story maps developed for astronaut training, and a web scene for visualizing LiDAR.

Jagge, Amy M.↗

Artemis Internal Science Team Update: Data and Software Development

Artemis will reestablish human presence on the Moon and lead to a new era of scientific discovery and exploration. Led by the National Aeronautics and Space Administration (NASA), Artemis includes a collaboration of space agencies and companies from around the world. In support of Artemis, a cross-disciplinary effort of science, engineering, mission operations, and human factors personnel is developing the best methods, facilities, and field locations to test hardware, train astronauts, and evaluate modern concepts of operations. This abstract, as part of the Artemis Internal Science Team (AIST), provides an update to the science-relevant data and software developments and integrated testing efforts that have occurred in 2022.

M. J. Miller↗

Artemis Internal Science Team Update: Hardware and Integrated Testing

The Artemis program will reestablish human presence on the Moon and lead to a new era of scientific discovery and exploration. Led by the National Aeronautics and Space Administration (NASA), the Artemis program is a collaboration of space agencies and companies from around the world. In support of the Artemis program a cross disciplinary effort integrating science, engineering, operations, and human factors is currently being developed to identify methods, facilities, and field locations to test hardware, train astronauts, and evaluate concepts of operations. NASA’s Science Mission Directorate (SMD) and Exploration Systems Development Mission Directorate (ESDMD) established a comprehensive Science Team structure to support the Artemis program. This structure includes 1) an Artemis Internal Science Team (AIST), 2) a Geology Team (GT) with additional Participating Scientists (PS) roles, and 3) Payload Teams (PT) for the inclusion of scientific instruments. The GT, PS and PT will be competitively selected for each mission as part of NASA’s Research Opportunities in Space and Earth Sciences (ROSES) solicitations. The AIST provides multi-mission continuity and has been working closely with Artemis program elements to best integrate science into all aspects of planning and development. This abstract serves to provide an AIST update on science relevant hardware developments and integrated testing efforts that occurred in 2022.

Trevor George Graff↗

Artemis Internal Science Team Update: Deployed Payloads

Artemis will reestablish human presence on the Moon and lead to a new era of scientific discovery and exploration. Led by the National Aeronautics and Space Administration (NASA), the Artemis effort includes a collaboration of space agencies and companies from around the world [1]. In support of Artemis, a cross-disciplinary effort of science, engineering, operations, and human factors personnel is currently developing the best methods, facilities, and field locations to test hardware, train astronauts, and evaluate concepts of operations. This abstract, as part of the Artemis Internal Science Team (AIST) [2], provides an update to NASA’s plans for the solicitation, development, and operations of deployed surface payloads. In calendar year 2023, NASA’s Science Mission Directorate will solicit proposals for instruments to be deployed by crew on the surface of the Moon (Fig. 1), beginning with Artemis III [3]. The Artemis III mission will land in the south polar region of the Moon, within 6º of latitude from the south pole, in the vicinity of both persistently illuminated and permanently shadowed areas of the Moon, with potential access to surface-accessible volatile deposits [4]. Several of the proposed landing regions are located among some of the oldest parts of the Moon, and together with the permanently shadowed regions, provide the opportunity to learn about the history of the Moon through previously unexplored lunar regions [5]. Deployed payloads will be a critical part of a notional program that captures the highest-priority science for Artemis III and provides the greatest feedforward to follow-on missions and the build-up to the Artemis Base Camp

R. C. Weber↗

Artemis Internal Science Team Update: Deployed Payloads

Artemis will reestablish human presence on the Moon and lead to a new era of scientific discovery and exploration. Led by the National Aeronautics and Space Administration (NASA), the Artemis effort includes a collaboration of space agencies and companies from around the world. In support of Artemis, a cross-disciplinary effort of science, engineering, operations, and human factors personnel is currently developing the best methods, facilities, and field locations to test hardware, train astronauts, and evaluate concepts of operations. This poster, as part of the Artemis Internal Science Team (AIST), provides an update to NASA’s plans for the solicitation, development, and operations of deployed surface payloads. In calendar year 2023, NASA’s Science Mission Directorate will solicit proposals for instruments to be deployed by crew on the surface of the Moon (Fig. 1), beginning with Artemis III. The Artemis III mission will land in the south polar region of the Moon, within 6º of latitude from the south pole, in the vicinity of both persistently illuminated and permanently shadowed areas of the Moon, with potential access to surface-accessible volatile deposits. Several of the proposed landing regions are located among some of the oldest parts of the Moon, and together with the permanently shadowed regions, provide the opportunity to learn about the history of the Moon through previously unexplored lunar regions. Deployed payloads will be a critical part of a notional program that captures the highest-priority science for Artemis III and provides the greatest feed-forward to follow-on missions and the build-up to the Artemis Base Camp.

R. C. Weber↗

Single Drop Cytometry Onboard the International Space Station

Real-time lab analysis is needed to support clinical decision making and research on human missions to the Moon and Mars. Powerful laboratory instruments, such as flow cytometers, are generally too cumbersome for spaceflight. Here, we show that scant test samples can be measured in microgravity, by a trained astronaut, using a miniature cytometry-based analyzer, the rHEALTH ONE, modified specifically for spaceflight. The base device addresses critical spaceflight requirements including minimal resource utilization and alignment-free optics for surviving rocket launch. To fully enable reduced gravity operation onboard the space station, we incorporated bubble-free fluidics, electromagnetic shielding, and gravity-independent sample introduction. We show microvolume flow cytometry from 10 μL sample drops, with data from five simultaneous channels using 10 μs bin intervals during each sample run, yielding an average of 72 million raw data points in approximately 2 minutes. We demonstrate the device measures each test sample repeatably, including correct identification of a sample that degraded in transit to the International Space Station. This approach can be utilized to further our understanding of spaceflight biology and provide immediate, actionable diagnostic information for management of astronaut health without the need for Earth-dependent analysis.

Daniel J. Rea↗

Digital Lunar Exploration Sites (DLES) Terrain Crafting

Humans will soon be returning to the surface of the Moon with NASA’s Artemis program. The Artemis program is an international collaboration that will consist of a complex series of space systems and missions to explore the lunar surface and pave the way for the future exploration of Mars. NASA and its partners rely heavily on simulation for lighting and navigation studies as well as training astronauts, flight controllers, and mission support staff. The NASA Exploration Systems Simulations (NExSyS) team in the Simulation and Graphics Branch (ER7) in the Engineering Directorate at NASA’s Johnson Space Center has built up many simulation products to support this effort, one of which is the Digital Lunar Exploration Sites (DLES). DLES is a collection of products used to simulate and render the lunar surface in a digital environment. We discussed and presented an overview of the DLES products at the 2022 IEEE Aerospace Conference in Big Sky, MT with a paper titled "Digital Lunar Exploration Sites". This “DLES Terrain Crafting” paper will expand on the information previously provided in “DLES” paper and dive deeper into the details of the terrain crafting process and the toolsets used to support this task. The best digital data currently available of the lunar surface is provided by the Lunar Reconnaissance Orbiter (LRO). Its Lunar Orbiter Laser Altimeter (LOLA) achieves an impressive resolution of 5m per pixel at the Lunar South Pole (LSP) and can generate datasets covering a large continuous region near the LSP. There are a few additional methods, such as Shape from Shading which can infer higher resolution data (up to 1m per pixel) from the LRO Narrow Angle Camera (NAC) images. However, surface-based simulations require higher-resolution data, and this paper will discuss the process of enhancing the terrain to meet that need. The process begins with capturing statistical data of craters in the regions of interest using images provided by the LRO NAC. This data is then used to scatter artificial features which are not captured in the truth data, resulting in an enhanced DEM with a much higher resolution of 20cm per pixel. Many tools were built up to assist in the creation of these artificial Digital Elevation Models (DEM), which this paper will discuss in detail. DEMs themselves are a very powerful representation of a planetary surface, and many operations and tools can utilize the data they contain. This paper includes a description of the rendering of the lunar surface in a graphics engine, generation of contact patches to simulate tire to ground interaction, and ray tracing utilities to model Line of Sight (LOS) interactions with the terrain. This paper will also explore some new tool sets currently under development which aim to utilize Machine Learning (ML) to assist in the identification of craters from LRO NAC imagery. While this is not a novel idea, the NExSyS team is developing a unique approach which may result in more robust identification of crater characteristics.

Artemis↗

Sensorimotor Countermeasures

Exploration class missions will include multiple transitions between gravitational environments, sometimes after long periods in microgravity, which will impact the neurovestibular system and sensorimotor capabilities. New countermeasures and assessment tools are needed to enhance preflight disorientation training, maintain inflight physical performance, and accelerate recovery following transitions between gravity environments so that crew are enabled to perform critical exploration mission tasks. The Sensorimotor Countermeasures capability area encompasses three projects. 1) The Upright Proprioception Retention via Inflight Training and Evaluation (UPRITE) system is being developed as an in-flight countermeasure that will mitigate the degradation of balance control mechanisms, which will improve post-flight postural stability. The development of such a countermeasure is not simply a matter of adapting a ground-based training program for spaceflight. The way that we control balance in a gravitational environment cannot be duplicated in 0g. Therefore, the UPRITE system challenges proprioception and tactile function using a 0g configuration. The steps to develop the countermeasure have been broken into seven phases: training type, controllable board factors, protocol development, 0g hardware test, training efficacy, ISS hardware demonstration, and countermeasure verification. 2) Unobtrusive Monitoring Tools and Operational Assessments (aka Head/Body Assessments) are being developed to quantify crew member sensorimotor adaptation following gravitational transitions. Three areas are needed in this development 1) explore, develop, and validate unobtrusive monitoring tools, 2) define sensorimotor performance metrics, and 3) explore capabilities to enhance sensorimotor assessment tasks for operational use and validate assessment tasks via high-fidelity analogs. 3) The Spatial Disorientation Trainer is being developed as a portable training capability to simulate the performance of landing and recovery type tasks while experiencing vertigo due to post-flight vestibular alterations. These simulations can be used 1) across research studies investigating the impacts of vestibular disruption on operational performance/assessments, 2) to train astronauts for upcoming spaceflight missions, and 3) to educate space medicine and operational personnel prior to providing post-flight support.

Scott Jonathan Wood↗

The Role and Training of NASA Astronauts in the Post-Shuttle Era

In May 2010 the National Research Council (NRC) was asked by NASA to address several questions related to the Astronaut Corps. The NRC's Committee on Human Spaceflight Crew Operations was tasked to: 1. How should the role and size of the activities managed by the Johnson Space Center Flight Crew Operations Directorate change following space shuttle retirement and completion of the assembly of the International Space Station (ISS)? 2. What are the requirements for crew-related ground-based facilities after the Space Shuttle program ends? 3. Is the fleet of aircraft used for training the Astronaut Corps a cost-effective means of preparing astronauts to meet the requirements of NASA's human spaceflight program? Are there more cost-effective means of meeting these training requirements? Although the future of NASA's human spaceflight program has garnered considerable discussion in recent years, and there is considerable uncertainty about what that program will involve in the coming years, the committee was not tasked to address whether or not human spaceflight should continue, or what form it should take. The committee's task restricted it to studying those activities managed by the Flight Crew Operations Directorate, or those closely related to its activities, such as crew-related ground-based facilities and the training aircraft.

Source record↗

Preparing for the High Frontier: The Role and Training of NASA Astronauts in the Post- Space Shuttle Era

In May 2010, the National Research Council (NRC) was asked by NASA to address several questions related to the Astronaut Corps. The NRC s Committee on Human Spaceflight Crew Operations was tasked to answer several questions: 1. How should the role and size of the activities managed by the Johnson Space Center Flight Crew Operations Directorate change after space shuttle retirement and completion of the assembly of the International Space Station (ISS)? 2. What are the requirements for crew-related ground-based facilities after the Space Shuttle program ends? 3. Is the fleet of aircraft used for training the Astronaut Corps a cost-effective means of preparing astronauts to meet the requirements of NASA s human spaceflight program? Are there more cost-effective means of meeting these training requirements? Although the future of NASA s human spaceflight program has garnered considerable discussion in recent years and there is considerable uncertainty about what the program will involve in the coming years, the committee was not tasked to address whether human spaceflight should continue or what form it should take. The committee s task restricted it to studying activities managed by the Flight Crew Operations Directorate or those closely related to its activities, such as crew-related ground-based facilities and the training aircraft.

Source record↗

A Review of Training Methods and Instructional Techniques: Implications for Behavioral Skills Training in U.S. Astronauts (DRAFT)

Long-duration space missions (LDM) place unique physical, environmental and psychological demands on crewmembers that directly affect their ability to live and work in space. A growing body of research on crews working for extended periods in isolated, confined environments reveals the existence of psychological and performance problems in varying degrees of magnitude. The research has also demonstrated that although the environment plays a cathartic role, many of these problems are due to interpersonal frictions (Wood, Lugg, Hysong, & Harm, 1999), and affect each individual differently. Consequently, crewmembers often turn to maladaptive behaviors as coping mechanisms, resulting in decreased productivity and psychological discomfort. From this body of research, critical skills have been identified that can help a crewmember better navigate the psychological challenges of long duration space flight. Although most people lack several of these skills, most of them can be learned; thus, a training program can be designed to teach crewmembers effective leadership, teamwork, and self-care strategies that will help minimize the emergence of maladaptive behaviors. Thus, it is the purpose of this report is twofold: 1) To review the training literature to help determine the optimal instructional methods to use in delivering psychological skill training to the U.S. Astronaut Expedition Corps, and 2) To detail the structure and content of the proposed Astronaut Expedition Corps Psychological Training Program.

Hysong, Sylvia J.↗

NASA astronauts

Qualifications and training of NASA astronauts

SPACE PROGRAM↗

Training of cosmonauts and astronauts

The biomedical and preflight training of spacecraft crews is discussed based on a survey of scientific and technical literature in the U.S. and U.S.S.R. Experience gained from high velocity and high altitude aircraft flights, predictions of human reactions and theoretical models of human adaptation to the new environment of space, and actual spaceflight experience provided scientists and specialists with data from which the state of human health in space could be predicted and life support measures developed.

Gurovskiy, N. N.↗

Development of an Inertial Sensor-based Methodology for Spacesuited Geology Task Assessments during Simulated Lunar Extravehicular Activities

Lunar surface exploration during Artemis missions will require the specific skill set of geology sampling. Apollo astronauts had extensive training and used specialized tools to collect lunar rocks, core samples, pebbles, sand, and dust. The inflexibility of the pressurized Apollo spacesuits forced sampling to be taken at a standstill posture. However, new exploration spacesuits are expected to incorporate advanced materials and joint bearings, allowing for greater mobility and a wider range of functional postures. Thus, science and exploration during Artemis missions will likely involve a variety of standing, squatting, and kneeling postures. In preparation for future lunar exploration missions, NASA provides geologic training to astronauts and other mission personnel. This professional training with a spacesuit in simulated lunar environments will enhance performance and reduce risk of injury to astronauts on the lunar surface. However, anecdotally, untrained or newly trained people wearing prototype planetary spacesuits have been observed to performing motions differently than a trained geologist would when conducting the same geology sampling tasks. Therefore, a tool for evaluating geology postures at extravehicular activity (EVA) training facilities becomes required. In this paper, we introduce a novel inertial measurement unit (IMU)-based method of geology task assessments in spacesuited conditions during simulated lunar EVAs. As a case study, two subjects (one geologist and one non-geologist) participated and donned the Mark III prototype planetary spacesuit during offloading with the spreader bar gimbal in NASA’s Active Response Gravity Offload System (ARGOS). For automated geology task assessments, the spacesuit was instrumented with three wireless IMUs (APDM Opal, OR, USA): one on the chest and one each on the left and right ankle bearings. Then subjects performed geology tasks using various tools (rake, trench, hammer chisel, scoop, and drive tube) for 45 minutes each. The chest IMU measured the torso tilt angle in the sagittal plane. We used an ensemble learning method with the ankle IMUs to discriminate between standing and kneeling activities. IMU data were processed using custom MATLAB (Mathworks, MA, USA) software. In our case study, the developed method was able to discriminate differences in standing and kneeling activity levels between subjects who were all highly experienced with spacesuited testing. Our preliminary data showed one subject maintained the constant and lower range of the upper body tilt angle while both standing and kneeling, while the other subject showed more variation of the upper body tilt angle and preferred bending the upper body rather than changing from standing to kneeling posture and vice versa. While geology experience may be a factor, these results need further investigation as suit sizing and ARGOS offloading configurations have been proven to have a significant influence on suited ARGOS tasks. Also, more subjects will be needed to complete these tasks for validation. IMU-based geology task assessments can provide useful information for geology training programs. Additionally, our IMU-based posture analysis can provide new insights into how to evaluate spacesuited geology task characteristics of astronauts during simulated lunar EVAs.

Kyoung Jae Kim↗