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At least 181 records · Page 10

Transcriptional control of monolignol biosynthesis in Pinus taeda: factors affecting monolignol ratios and carbon allocation in phenylpropanoid metabolism

Transcriptional profiling of the phenylpropanoid pathway in Pinus taeda cell suspension cultures was carried out using quantitative real time PCR analyses of all known genes involved in the biosynthesis of the two monolignols, p-coumaryl and coniferyl alcohols (lignin/lignan precursors). When the cells were transferred to a medium containing 8% sucrose and 20 mm potassium iodide, the monolignol/phenylpropanoid pathway was induced, and transcript levels for phenylalanine ammonia lyase, cinnamate 4-hydroxylase, p-coumarate 3-hydroxylase, 4-coumarate:CoA ligase, caffeoyl-CoA O-methyltransferase, cinnamoyl-CoA reductase, and cinnamyl alcohol dehydrogenase were coordinately up-regulated. Provision of increasing levels of exogenously supplied Phe to saturating levels (40 mm) to the induction medium resulted in further up-regulation of their transcript levels in the P. taeda cell cultures; this in turn was accompanied by considerable increases in both p-coumaryl and coniferyl alcohol formation and excretion. By contrast, transcript levels for both cinnamate 4-hydroxylase and p-coumarate 3-hydroxylase were only slightly up-regulated. These data, when considered together with metabolic profiling results and genetic manipulation of various plant species, reveal that carbon allocation to the pathway and its differential distribution into the two monolignols is controlled by Phe supply and differential modulation of cinnamate 4-hydroxylase and p-coumarate 3-hydroxylase activities, respectively. The coordinated up-regulation of phenylalanine ammonia lyase, 4-coumarate:CoA ligase, caffeoyl-CoA O-methyltransferase, cinnamoyl-CoA reductase and cinnamyl alcohol dehydrogenase in the presence of increasing concentrations of Phe also indicates that these steps are not truly rate-limiting, because they are modulated according to metabolic demand. Finally, the transcript profile of a putative acid/ester O-methyltransferase, proposed as an alternative catalyst for O-methylation leading to coniferyl alcohol, was not up-regulated under any of the conditions employed, suggesting that it is not, in fact, involved in monolignol biosynthesis.

NASA Discipline Plant Biology

Control and modeling of a CELSS (Controlled Ecological Life Support System)

Research topics that arise from the conceptualization of control for closed life support systems which are life support systems in which all or most of the mass is recycled are discussed. Modeling and control of uncertain and poorly defined systems, resource allocation in closed life support systems, and control structures or systems with delay and closure are emphasized.

Auslander, D. M.

Large Divergence of Projected High Latitude Vegetation Composition and Productivity Due To Functional Trait Uncertainty

Abstract Vegetation distribution and composition are expected to change in northern high latitudes under rapid warming, which regulates ecosystem functions but remains challenging to predict. Vegetation change arises from the interplay of chronic climate trends such as warming and transient demographic processes of recruitment, growth, competition, and mortality. Most predictive models overlooked the role of demographic dynamics controlled by plant traits. Here, we simulate vegetation dynamics at the Kougarok Hillslope site in Alaska under historical and future climates using the E3SM Land Model coupled to the Functionally Assembled Terrestrial Simulator (ELM‐FATES). To evaluate the roles of plant traits, we parameterize the model with 5,265 trait configurations representing diverse physiological and demographic strategies. Results show current modeled biomass, composition, and productivity are most sensitive to traits controlling photosynthetic capacity, carbon allocation, allometry, and phenology. Among all trait configurations, ∼5% reproduce in situ biomass and plant functional type (PFT) composition measured in 2016, that are indistinguishable from these two observed ecosystem states. Notably, these same trait configurations produce diverging biomass, composition, and productivity under future climate, where the uncertainty attributable to traits is twice the change attributable to climate change. The variation of projected productivity arises from emerging PFT composition under novel climate regimes, primarily explained by traits controlling cold‐induced mortality, recruitment, and allometry. Our findings highlight the importance and uncertainty of demographic dynamics and its interaction with climate change in shaping Arctic vegetation change. Improved model predictions will likely benefit from explicit consideration of vegetation demography and better constraints of critical traits.

54 ENVIRONMENTAL SCIENCES

Controlling Attitude of a Solar-Sail Spacecraft Using Vanes

A paper discusses a concept for controlling the attitude and thrust vector of a three-axis stabilized Solar Sail spacecraft using only four single degree-of-freedom articulated spar-tip vanes. The vanes, at the corners of the sail, would be turned to commanded angles about the diagonals of the square sail. Commands would be generated by an adaptive controller that would track a given trajectory while rejecting effects of such disturbance torques as those attributable to offsets between the center of pressure on the sail and the center of mass. The controller would include a standard proportional + derivative part, a feedforward part, and a dynamic component that would act like a generalized integrator. The controller would globally track reference signals, and in the presence of such control-actuator constraints as saturation and delay, the controller would utilize strategies to cancel or reduce their effects. The control scheme would be embodied in a robust, nonlinear algorithm that would allocate torques among the vanes, always finding a stable solution arbitrarily close to the global optimum solution of the control effort allocation problem. The solution would include an acceptably small angle, slow limit-cycle oscillation of the vanes, while providing overall thrust vector pointing stability and performance.

Mettler, Edward

Proceedings, 13th Annual Conference on Manual Control

Theoretical aspects of manual control theory are discussed. Specific topics covered include: tracking; performance, attention allocation, and mental load; surface vehicle control; monitoring behavior and supervisory control; manipulators and prosthetics; aerospace vehicle control; motion and visual cues; and displays and controls.

Source record

Cooperative Data Sharing: Simple Support for Clusters of SMP Nodes

Libraries like PVM and MPI send typed messages to allow for heterogeneous cluster computing. Lower-level libraries, such as GAM, provide more efficient access to communication by removing the need to copy messages between the interface and user space in some cases. still lower-level interfaces, such as UNET, get right down to the hardware level to provide maximum performance. However, these are all still interfaces for passing messages from one process to another, and have limited utility in a shared-memory environment, due primarily to the fact that message passing is just another term for copying. This drawback is made more pertinent by today's hybrid architectures (e.g. clusters of SMPs), where it is difficult to know beforehand whether two communicating processes will share memory. As a result, even portable language tools (like HPF compilers) must either map all interprocess communication, into message passing with the accompanying performance degradation in shared memory environments, or they must check each communication at run-time and implement the shared-memory case separately for efficiency. Cooperative Data Sharing (CDS) is a single user-level API which abstracts all communication between processes into the sharing and access coordination of memory regions, in a model which might be described as "distributed shared messages" or "large-grain distributed shared memory". As a result, the user programs to a simple latency-tolerant abstract communication specification which can be mapped efficiently to either a shared-memory or message-passing based run-time system, depending upon the available architecture. Unlike some distributed shared memory interfaces, the user still has complete control over the assignment of data to processors, the forwarding of data to its next likely destination, and the queuing of data until it is needed, so even the relatively high latency present in clusters can be accomodated. CDS does not require special use of an MMU, which can add overhead to some DSM systems, and does not require an SPMD programming model. unlike some message-passing interfaces, CDS allows the user to implement efficient demand-driven applications where processes must "fight" over data, and does not perform copying if processes share memory and do not attempt concurrent writes. CDS also supports heterogeneous computing, dynamic process creation, handlers, and a very simple thread-arbitration mechanism. Additional support for array subsections is currently being considered. The CDS1 API, which forms the kernel of CDS, is built primarily upon only 2 communication primitives, one process initiation primitive, and some data translation (and marshalling) routines, memory allocation routines, and priority control routines. The entire current collection of 28 routines provides enough functionality to implement most (or all) of MPI 1 and 2, which has a much larger interface consisting of hundreds of routines. still, the API is small enough to consider integrating into standard os interfaces for handling inter-process communication in a network-independent way. This approach would also help to solve many of the problems plaguing other higher-level standards such as MPI and PVM which must, in some cases, "play OS" to adequately address progress and process control issues. The CDS2 API, a higher level of interface roughly equivalent in functionality to MPI and to be built entirely upon CDS1, is still being designed. It is intended to add support for the equivalent of communicators, reduction and other collective operations, process topologies, additional support for process creation, and some automatic memory management. CDS2 will not exactly match MPI, because the copy-free semantics of communication from CDS1 will be supported. CDS2 application programs will be free to carefully also use CDS1. CDS1 has been implemented on networks of workstations running unmodified Unix-based operating systems, using UDP/IP and vendor-supplied high- performance locks. Although its inter-node performance is currently unimpressive due to rudimentary implementation technique, it even now outperforms highly-optimized MPI implementation on intra-node communication due to its support for non-copy communication. The similarity of the CDS1 architecture to that of other projects such as UNET and TRAP suggests that the inter-node performance can be increased significantly to surpass MPI or PVM, and it may be possible to migrate some of its functionality to communication controllers.

DiNucci, David C.

Distributed processing on the Space Shuttle - A case study

A Power Extension Package (PEP) has been designed to provide additional electrical power and energy during Shuttle sortie missions. The considered investigation was conducted to determine the most suitable allocation of PEP monitoring and control functions between the Orbiter's existing (centralized) Systems Management General Purpose Computer and an embedded PEP processor. PEP monitoring and control functions are examined, and a configuration definition is considered, taking into account the 'functional migration' process, function allocation criteria, and candidate functional configurations. A trade study is conducted, giving attention to an assessment of four candidate configurations. Assessment factors are related to cost, development risk, aspects of reliability and safety, PEP design complexity, PEP/STS integration complexity, flight operations, and launch/landing site operations. A thorough (subjective) assessment of the PEP system life cycle indicates substantial benefits from a distributed processing approach.

Schoonmaker, P. B.

A Closer Look at Automation Behavior During a Human-in-the-Loop Simulation

A 2012 Human-In-The-Loop air traffic control simulation investigated a gradual paradigm-shift in the allocation of functions between operators and automation. Air traffic controllers staffed five adjacent high-altitude en route sectors, and during the course of a two-week experiment, worked traffic under four different function allocation concepts aligned with increasingly mature NextGen operational environments. These NextGen time-frames ranged from near current-day operations to nearly fully-automated control, in which the ground systems automation was responsible for detecting conflicts, issuing strategic and tactical resolutions, and alerting controllers to exceptional circumstances. This paper continues the investigations reported in previous publications. Analyses of data surrounding the conflict-resolution task serve as the context in which we investigate the interactions between controllers and the automation.

Human-Automation Interaction

Nine Canyon Long-Duration Energy Storage: A Feasibility Study

The Nine Canyon Long Duration Energy Storage (LDES) Feasibility Study explores the technical and economic viability of deploying advanced energy storage technologies at Energy Northwest's (EN) Nine Canyon (9C) Wind Project site in Benton County, Washington. Supported by the Washington State Department of Commerce and the U.S. Department of Energy’s Office of Electricity under its LDES Voucher Program, the study represents a collaborative effort between EN, Pacific Northwest National Laboratory (PNNL), and ARES North America. At the core of this effort is the development of a generalized techno-economic modeling framework and evaluation tool designed to assess the value proposition of LDES projects across a variety of contexts. The modeling tool is technology-agnostic and accommodates user-defined parameters such as rated power, energy duration, round-trip efficiency, capital and operational costs, and dispatch constraints. It also integrates economic inputs, including market prices, energy revenue structures, and financing parameters to evaluate performance through key metrics. The tool provides utilities with a transparent, adaptable platform to support decision-making, investment prioritization, and portfolio planning for various storage technologies. To guide scenario design and interpretation, the study first surveyed the LDES technology landscape, including lithium-ion batteries, flow batteries, non-hydro gravity storage, and thermo-mechanical systems, comparing cost trajectories, technical performance, safety and hazards, materials sourcing and recyclability, and spatial/siting considerations. This literature-grounded review highlights technology trade-offs and reinforces the need to align technology choice with site characteristics, use cases, and project objectives. A companion chapter examines ownership structures (EN ownership, third-party ownership, shared models) and offtake options (energy marketing, capacity/energy PPAs, time-of-use PPAs, block-delivery PPAs, and tolling), where PPAs (power purchase agreements) represent contractual arrangements for buying and selling electricity. The chapter also highlights implications for risk allocation, capital access, operational control, and revenue certainty. The study also evaluates supervisory control and data acquisition (SCADA) and transmission interconnection pathways, options include upgrading the existing SCADA or deploying a dedicated LDES controller, with attention to protection schemes, data telemetry, cybersecurity, and regulatory coordination with BPA. In addition, an ARES-specific geotechnical and hydrology assessment presented in the appendix screens multiple corridors for slope stability, bearing capacity, cut-and-fill magnitude, and stormwater behavior.

25 ENERGY STORAGE

Impacts of solar energy utilization

Various methods of conducting surveys and analyses to determine the attitude of the public toward the energy crisis are discussed. Models to determine the impact of the energy crisis and proposed alternative sources of energy on the social structure are analyzed. The various interest groups which are concerned with energy and the nature of their interest are identified. The government structure for controlling resource production and allocation is defined.

Source record

An inside look at NASA planetology

Staffing, financing and budget controls, and research grant allocations of NASA are reviewed with emphasis on NASA-supported research in planetary geological sciences: studies of the composition, structure, and history of solar system planets. Programs, techniques, and research grants for studies of Mars photographs acquired through Mariner 6-10 flights are discussed at length, and particularly the handling of computer-enhanced photographic data. Scheduled future NASA-sponsored planet exploration missions (to Mars, Jupiter, Saturn, Uranus) are mentioned.

Dwornik, S. E.

WARC 92 and some thoughts as to its impact on the NASA propagation program

The World Administrative Radio Conference of 1992 (WARC 92) was held in Torremolinos, Spain, 3 Feb. - 3 Mar. 1992. Major topics considered included shortwave broadcasting, mobile and mobile-satellite service, broadcasting satellite service (sound and HDTV), space services above 20 GHz, and space research. Considerable attention was given to the congested 1-3 GHz band in general and to Low Earth Orbit (LEO) Mobile-Satellite Service, including 'little' LEO's operating below 1 GHz and to 'big' LEO's operating above 1 GHz. Significant new allocations were made for generic Mobile-Satellite Services (MSS). Proposals for allocations for uplink power control beacons and for space research received favorable treatment.

Flock, Warren L.

Space transportation alternatives for large space programs - The International Space University summer session - 1992

The issues discussed in this paper are the result of a 10-week study by the Space Solar Power Program design project members and the Space Transportation Group at the International Space University (ISU) summer session of 1992 to investigate new paradigms in space propulsion and how those paradigms might reduce the costs for large space programs. The program plan was to place a series of power satellites in Earth orbit. Several designs were studied where many kW, MW or GW of power would be transmitted to Earth or to other spacecraft in orbit. During the summer session, a space solar power system was also detailed and analyzed. At ISU, the focus of the study was to foster and develop some of the new paradigms that may eliminate the barriers to low cost for space exploration and exploitation. Many international and technical aspects of a large multinational program were studied. Environmental safety, space construction and maintenance, legal and policy issues of frequency allocation, technology transfer and control and many other areas were addressed.

Palaszewski, Bryan

Space transportation alternatives for large space programs: The International Space University Summer Session, 1992

In 1992, the International Space University (ISU) held its Summer Session in Kitakyushu, Japan. This paper summarizes and expands upon some aspects of space solar power and space transportation that were considered during that session. The issues discussed in this paper are the result of a 10-week study by the Space Solar Power Program design project members and the Space Transportation Group to investigate new paradigms in space propulsion and how those paradigms might reduce the costs for large space programs. The program plan was to place a series of power satellites in Earth orbit. Several designs were studied where many kW, MW, or GW of power would be transmitted to Earth or to other spacecraft in orbit. During the summer session, a space solar power system was also detailed and analyzed. A high-cost space transportation program is potentially the most crippling barrier to such a space power program. At ISU, the focus of the study was to foster and develop some of the new paradigms that may eliminate the barriers to low cost for space exploration and exploitation. Many international and technical aspects of a large multinational program were studied. Environmental safety, space construction and maintenance, legal and policy issues of frequency allocation, technology transfer and control and many other areas were addressed. Over 120 students from 29 countries participated in this summer session. The results discussed in this paper, therefore, represent the efforts of many nations.

Palaszewski, Bryan A.

A Model-based Approach to Controlling the ST-5 Constellation Lights-Out Using the GMSEC Message Bus and Simulink

Space Technology 5 (ST-5) is a three-satellite constellation, technology validation mission under the New Millennium Program at NASA to be launched in March 2006. One of the key technologies to be validated is a lights-out, model-based operations approach to be used for one week to control the ST-5 constellation with no manual intervention. The ground architecture features the GSFC Mission Services Evolution Center (GMSEC) middleware, which allows easy plugging in of software components and a standardized messaging protocol over a software bus. A predictive modeling tool built on MatLab's Simulink software package makes use of the GMSEC standard messaging protocol to interface to the Advanced Mission Planning System (AMPS) Scenario Scheduler which controls all activities, resource allocation and real-time re-profiling of constellation resources when non-nominal events occur. The key features of this system, which we refer to as the ST-5 Simulink system, are as follows: Original daily plan is checked to make sure that predicted resources needed are available by comparing the plan against the model. As the plan is run in real-time, the system re-profiles future activities in real-time if planned activities do not occur in the predicted timeframe or fashion. Alert messages are sent out on the GMSEC bus by the system if future predicted problems are detected. This will allow the Scenario Scheduler to correct the situation before the problem happens. The predictive model is evolved automatically over time via telemetry updates thus reducing the cost of implementing and maintaining the models by an order of magnitude from previous efforts at GSFC such as the model-based system built for MAP in the mid-1990's. This paper will describe the key features, lessons learned and implications for future missions once this system is successfully validated on-orbit in 2006.

Witt, Kenneth J.

Development of a Smooth Trajectory Maneuver Method to Accommodate the Ares I Flight Control Constraints

Six degree-of-freedom (DOF) launch vehicle trajectories are designed to follow an optimized 3-DOF reference trajectory. A vehicle has a finite amount of control power that it can allocate to performing maneuvers. Therefore, the 3-DOF trajectory must be designed to refrain from using 100% of the allowable control capability to perform maneuvers, saving control power for handling off-nominal conditions, wind gusts and other perturbations. During the Ares I trajectory analysis, two maneuvers were found to be hard for the control system to implement; a roll maneuver prior to the gravity turn and an angle of attack maneuver immediately after the J-2X engine start-up. It was decided to develop an approach for creating smooth maneuvers in the optimized reference trajectories that accounts for the thrust available from the engines. A feature of this method is that no additional angular velocity in the direction of the maneuver has been added to the vehicle after the maneuver completion. This paper discusses the equations behind these new maneuvers and their implementation into the Ares I trajectory design cycle. Also discussed is a possible extension to adjusting closed-loop guidance.

Pinson, Robin M.

Workload and Performance in Air Traffic Control: Exploring the Influence of Levels of Automation and Variation in Task Demand

In air traffic control, task demand and workload have important implications for the safety and efficiency of air traffic. Task demand is dynamic, however, research on demand transitions and associated controller perception and performance is limited. In addition, there is a comparatively restricted understanding of the influence of task demand transitions on workload and performance, in association with automation. This study used an air traffic control simulation to investigate the influence of task demand transitions and two conditions of varying automation, on workload and efficiency-related performance. Findings showed that a both the direction of the task demand variation, and the amount of automation, influenced the relationship between workload and performance. Further research is needed to enhance understanding of demand transition and workload history effects on operator experience and performance, in both air traffic control and other safety-critical domains.

air traffic control

How Does Escherichia coli Allocate Proteome?

Microorganisms are shown to actively partition their intracellular resources, such as pro- teins, for growth optimization. Recent experiments have begun to reveal molecular com- ponents unpinning the partition; however, it remains unclear quantitatively how individual parts orchestrate to yield precise resource allocation that is both robust and dynamic. Here we developed a coarse-grained mathematical framework that centers on guanosine pentaphosphate (ppGpp)-mediated regulation, and used it to systematically uncover the design principles of proteome allocation in Escherichia coli. Our results showed that cellular ability of resource partition lies in an ultrasensitive, negative feedback control- ling topology with the ultrasensitivity arising from zero-order amino acid kinetics and the negative feedback from ppGpp-controlled ribosome synthesis. In addition, together with the time-scale separation between slow ribosome kinetics and fast turnovers of ppGpp and amino acids, the network topology confers the organism an optimization mechanism which mimics sliding mode control, a nonlinear optimization strategy that is widely used in man-made systems. We further showed that such a controlling mechanism is robust against parameter variations and molecular fluctuations, and is also efficient for biomass production over time. Furthermore, this work elucidates the fundamental controlling mechanism of E. coli proteome allocation, thereby providing insights into quantitative microbial physiology as well as the design of synthetic gene networks.

59 BASIC BIOLOGICAL SCIENCES