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At least 181 records · Page 10

Culture-Based Environmental Microbiology Monitoring of Crop-Based Space Food Systems (veggie Monitoring)

Crewmembers live and work in a closed environment that is monitored to ensure their health and safety. Quarterly monitoring of the microorganisms in the International Space Station (ISS) environment supports crew safety and contributes to a large set of microbial concentration and diversity data from air, surfaces and water samples. This study leverages quarterly operational Environmental Health System (EHS) sampling by collecting additional microbial samples from the surface of the station’s Veggie plant production system. Longer exploration missions may require spaceflight-based systems for growth of plants, and this investigation is expected to provide additional data to help establish requirements to protect these systems, plants, and crew, mitigating adverse microbial exposure.

Hamilton, Tanner↗

2022 Annual Report: Culture-Based Environmental Microbiology Monitoring of Crop-based Space Food Systems (Veggie Monitoring)

Crewmembers live and work in a closed environment that is monitored to ensure their health and safety. Quarterly monitoring of the microorganisms in the International Space Station (ISS) environment supports crew safety and contributes to a large set of environmental microbial data from the air, surface, and water samples that are collected. This study leverages quarterly operational Environmental Health System (EHS) sampling by collecting additional microbial samples from the surface of the station’s Veggie plant production system. Longer exploration missions may require spaceflight-based systems for growth of plants, and this investigation is expected to provide additional data to help establish requirements to protect these systems, plants, and crew, mitigating adverse microbial exposure.

Tanner Hamilton↗

Continued Environmental Microbiology Monitoring of The International Space Station (ISS) Veggie Unit Used for In-Flight, Crop-Based Food Systems

The International Space Station is a closed environment where rotating sets of Crewmembers live and work. This environment is monitored to ensure occupants’ health and safety during their spaceflight residency by routine Environmental Health System (EHS) collection of microbial samples including air, surface, and water. The microbial samples are collected, enumerated, and analyzed quarterly to monitor on-board system contamination and potential risks to crew health. Quarterly monitoring of the microorganisms in the ISS environment supports crew safety and contributes to a large set of microbial concentration and diversity data. The current in-inflight microbial requirements were developed using this historical data collected by the routine environmental monitoring. These in-flight microbial requirements have been established to maintain the health and safety of the spacecraft environment. This study leverages quarterly operational EHS sampling by collecting additional microbial samples from the surface of the Veggie plant production system on ISS. These samples will yield microbial concentration and diversity that can be compared and analyzed with nominal surface samples from the vehicle. The data collected in this study will aid in the development of requirements for spaceflight-based food production systems. Continued surface sampling of the internal and external surfaces of the Veggie system, along with collaboration from both Johnson Space Center (JSC) & Kennedy Space Center (KSC) scientists studying the microbiome of the veggie-crop systems, will be implemented as part of the future development of crop-based food system requirements for the ISS and beyond.

Christian Mena↗

A Microbial Analysis of Space-Grown Produce

Before space crops become a permanent staple of the astronaut diet, we must first understand how plants and pathogenic microbes interact in microgravity. Crops such as red romaine lettuce and Chinese cabbage were grown on the International Space Station and sent back to Kennedy Space Center for microbial analysis. Aerobic plate counts and metagenomic sequencing were used to characterize bacterial and fungal communities for plants and their respective ''pillows''. These data will be used to create new guidelines for the microbial safety of space-grown produce, and will help us better protect astronauts from food-borne pathogens like E.coli, Staphylococcus, and Salmonella.

Barash, Eric L.↗

Toward net-zero sustainable aviation fuel with wet waste–derived volatile fatty acids

Significance To meet the growing demand for sustainable aviation fuels (SAF), conversion pathways are needed that leverage wet waste carbon and meet jet fuel property specifications. Here, we demonstrate SAF production from food waste–derived volatile fatty acids (VFA) by targeting normal paraffins for a near-term path to market and branched isoparaffins to increase the renewable content long term. Combining these distinct paraffin structures was shown to synergistically improve VFA-SAF flash point and viscosity to increase the renewable blend limit to 70%. Life cycle analysis shows the dramatic impact on the carbon footprint if food waste is diverted from landfills to produce VFA-SAF, highlighting the potential to meet jet fuel safety, operability, and environmental goals.

09 BIOMASS FUELS↗

Magnetic Detection of Chemical Threats

Los Alamos researchers have developed a technology to quickly detect chemical threats using a fieldable detector that provides real-time detection to ensure safety for warfighters, agricultural workers, and the general public. Traditional laboratory-based detection does not allow for agile response to threats on the battlefield or to our food supply chain. Our portable detector brings the laboratory to the field for real-time detection of chemical agents in samples without needing to send the samples to a remote location for analysis. This technology fits a market need in public safety, military sensor design, and pesticide detection. We have proven that this technology works on the benchtop, and are working to miniaturize the system to enable deployment. We are seeking qualified licensing or CRADA partners to finalize development of this system.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗

An Integrated 0-1 Hour First-Flash Lightning Nowcasting, Lightning Amount and Lightning Jump Warning Capability

Lightning one of the most dangerous weather-related phenomena, especially as many jobs and activities occur outdoors, presenting risk from a lightning strike. Cloud-to-ground (CG) lightning represents a considerable safety threat to people at airfields, marinas, and outdoor facilities-from airfield personnel, to people attending outdoor stadium events, on beaches and golf courses, to mariners, as well as emergency personnel. Holle et al. (2005) show that 90% of lightning deaths occurred outdoors, while 10% occurred indoors despite the perception of safety when inside buildings. Curran et al. (2000) found that nearly half of fatalities due to weather were related to convective weather in the 1992-1994 timeframe, with lightning causing a large component of the fatalities, in addition to tornadoes and flash flooding. Related to the aviation industry, CG lightning represents a considerable hazard to baggage-handlers, aircraft refuelers, food caterers, and emergency personnel, who all become exposed to the risk of being struck within short time periods while convective storm clouds develop. Airport safety protocols require that ramp operations be modified or discontinued when lightning is in the vicinity (typically 16 km), which becomes very costly and disruptive to flight operations. Therefore, much focus has been paid to nowcasting the first-time initiation and extent of lightning, both of CG and of any lightning (e.g, in-cloud, cloud-to-cloud). For this project three lightning nowcasting methodologies will be combined: (1) a GOESbased 0-1 hour lightning initiation (LI) product (Harris et al. 2010; Iskenderian et al. 2012), (2) a High Resolution Rapid Refresh (HRRR) lightning probability and forecasted lightning flash density product, such that a quantitative amount of lightning (QL) can be assigned to a location of expected LI, and (3) an algorithm that relates Pseudo-GLM data (Stano et al. 2012, 2014) to the so-called "lightning jump" (LJ) methodology (Shultz et al. 2011) to monitor lightning trends and to anticipate/forecast severe weather (hail > or =2.5 cm, winds > or =25 m/s, tornadoes). The result will be a time-continuous algorithm that uses GOES satellite, radar fields, and HRRR model fields to nowcast first-flash LI and QL, and subsequently monitors lightning trends on a perstorm basis within the LJ algorithm for possible severe weather occurrence out to > or =3 hours. The LI-QL-LJ product will also help prepare the operational forecast community for Geostationary Lightning Mapper (GLM) data expected in late 2015, as these data are monitored for ongoing convective storms. The LI-QL-LJ product will first predict where new lightning is highly probable using GOES imagery of developing cumulus clouds, followed by n analysis of NWS (dual-polarization) radar indicators (reflectivity at the -10 C altitude) of lightning occurrence, to increase confidence that LI is immanent. Once lightning is observed, time-continuous lightning mapping array and Pseudo-GLM observations will be analyzed to assess trends and the severe weather threat as identified by trends in lightning (i.e. LJs). Additionally, 5- and 15-min GOES imagery will then be evaluated on a per-storm basis for overshooting and other cloud-top features known to be associated with severe storms. For the processing framework, the GOES-R 0-1 hour convective initiation algorithm's output will be developed within the Warning Decision Support System - Integrated Information (WDSS-II) tracking tool, and merged with radar and lightning (LMA/Psuedo-GLM) datasets for active storms. The initial focus of system development will be over North Alabama for select lightning-active days in summer 2014, yet will be formed in an expandable manner. The lightning alert tool will also be developed in concert with National Weather Service (NWS) forecasters to meet their needs for real-time, accurate first-flash LI and timing, as well as anticipated lightning trends, amounts, continuation and cessation, so to provide key situational awareness and decision support information. The NASA Short-term Prediction Research and Transition (SPoRT) Center will provide important logistical and collaborative support and training, involving interactions with the NWS and broader user community.

Mecikalski, John↗

BioNutrients-3: Precision Fermentation, Pasteurization, and Pathogen Detection - Towards Safe Fermentation and Production of Nutrients in Space

The BioNutrients (BN) project is developing a microbial manufacturing approach to supplement the NASA food system to address known nutrient degradation associated with long-term storage. BN uses synthetic biology to deliver high-value nutrients and therapeutics through genetic engineering of microbes and production of fermented food products like yogurt and kefir, in a fully dehydrated system. On-demand production of nutrients for human consumption requires rigorous safety protocols to ensure contaminants are not introduced during the fermentation process. The third iteration of the BN flight project, BN-3, further develops the BN project by investigating strategies for pathogen detection and pasteurization in microgravity. BN-3 will test the limits of pathogen detection using whole genome sequencing, standard microbial assays and the NASA Ames E-Nose, a volatile carbon nano tube-based sensor array, to detect unwanted microbes. BN-3 also expands on the number of nutrients produced in a single bioreactor to enhance efficiency of the system by combining production of B vitamins in conjunction with the carotenoids, beta-carotene and zeaxanthin. Serial production of food products through yogurt passaging, new updates to the fluorinated ethylene propylene bags to allow crew access via a straw, as well as use of a food safe pH indicator dye to indicate readiness of the food product, will further enhance the useability of this system. This presentation will provide status of the BN-3 flight project with the aim of advancing in-space biomanufacturing for on-demand microbially based food production for future space exploration.

Biomanufacturing↗

Skylab Medical Experiments Altitude Test /SMEAT/ facility design and operation.

This paper presents the design approaches and test facility operation methods used to successfully accomplish a 56-day test for Skylab to permit evaluation of selected Skylab medical experiments in a ground test simulation of the Skylab environment with an astronaut crew. The systems designed for this test include the two-gas environmental control system, the fire suppression and detection system, equipment transfer lock, ground support equipment, safety systems, potable water system, waste management system, lighting and power system, television monitoring, communications and recreation systems, and food freezer.

Hinners, A. H., Jr.↗

Mitigating the allergenicity of peanut allergen Ara h 1 by cold atmospheric pressure argon plasma jet

Abstract BACKGROUND Peanut allergy is recognized as a major food allergy that triggers severe and even fatal symptoms. Avoidance of peanuts in the diet is the main option for current safety management. Processing techniques reducing peanut allergenicity are required to develop other options. Cold plasma is currently considered as a novel non‐thermal approach to alter protein structure and has the potential to alleviate immunoreactivity of protein allergen. RESULTS The application of a cold argon plasma jet to peanut protein extract could reduce the amount of a 64 kDa protein band corresponding to a major peanut allergen Ara h 1 using sodium dodecyl sulfate–polyacrylamide gel electrophoresis, but the overall protein size distribution did not change significantly. A decrease in peanut protein solubility was a possible cause that led to the loss of protein content in the soluble fraction. Immunoblotting and enzyme‐linked immunosorbent assay elucidated that the immunoreactivity of Ara h 1 was significantly decreased with the time treated with plasma. Ara h 1 antigenicity reduced by 38% after five scans (approximately 3 min) of cold argon plasma jet treatment, and the reduction was up to 66% after approximately 15 min of treatment. CONCLUSION The results indicate that cold argon plasma jet treatment could be a suitable platform for alleviating the immunoreactivity of peanut protein. This work demonstrates an efficient, compact, and rapid platform for mitigating the allergenicity of peanuts, and shows great potential for the plasma platform as a non‐thermal technique in the food industry. © 2023 Society of Chemical Industry.

Hsu, Fu‐Chiun↗

Research planning criteria for regenerative life-support systems applicable to space habitats

The second phase of analyses that were conducted by the Life Support Systems Group of the 1977 NASA Ames Summer Study is described. This phase of analyses included a preliminary review of relevant areas of technology that can contribute to the development of closed life-support systems for space habitats, the identification of research options in these areas of technology, and the development of guidelines for an effective research program. The areas of technology that were studied included: (1) nutrition, diet, and food processing; (2) higher plant agriculture; (3) animal agriculture; (4) waste conversion and resource recovery; and (5) system stability and safety. Results of these analyses, including recommended research options and criteria for establishing research priorities among these many options, are discussed.

Spurlock, J.↗

Nutritional Biochemistry of Spaceflight

Adequate nutrition is critical for crew health and safety during spaceflight. To ensure adequate nutrition, the nutrient requirements need to be both accurate and available from the spaceflight food system. The existing nutritional requirements for extended-duration spaceflight have been defined largely by extrapolation from ground-based research. However, nutritional requirements are influenced by most of the physiological consequences of spaceflight, including loss of lean, adipose, and bone tissue; changes in blood composition; and increased risk of renal stone formation. This review focuses on key areas where information has been gained in recent years: dietary intake and energy metabolism, bone health, fluid and electrolyte homeostasis, and hematological changes. Areas in which specific nutrients have the potential to serve as countermeasures to the negative effects of spaceflight are also reviewed. Dietary Intake

Smith, Scott M.↗

A Review of Polymeric Nanoparticle Design, Synthesis, and Biomedical Applications

Polymeric nanoparticles (PNPs) are solid particles that contain macromolecular polymers ranging in size from 1-1000 nm. They have a wide variety of structures that are determined by their identity as either natural biopolymers, synthetic polymers or a combination of both. In the design of PNPs, the polymeric system, size, internal structure, additional molecules, and synthetic pathway can be engineered, allowing for a multitude of properties including differing responses to physiological environments and the incorporation of a drug. The easily accessible fine tuning of PNPs allows for a multitude of applications in medicine. PNPs allow for increased pharmacokinetics, efficacy, safety, and targeting strength when utilized as carriers for drugs. Currently there are a wide range of PNPs approved by the Food and Drug Administration for use in clinical settings and many more being investigated in clinical studies. There is also research that is being conducted for the usage of PNPs in controlled drug delivery systems and as carriers through the blood brain barrier to aid in treating neurological diseases.

60 APPLIED LIFE SCIENCES↗

A building performance-based approach to determining energy resilience for grocery stores in the event of a power outage

Evaluating progress toward a built environment that is best equipped to serve communities during a regional power outage will require metrics that capture the energy resilience of the unique buildings and businesses most crucial to the well-being of those nearby. We focused on grocery stores as key buildings for which access, and thus energy resilience, is critical during a disaster when power is unavailable. We evaluated the energy resilience of these buildings by offering and testing building-scale metrics that assess business continuity potential during a power outage. Metrics proposed in this study are calculated based on the unique power loads characteristic to grocery stores, primarily refrigeration and maintaining safe indoor environmental conditions. Building simulations based on varying levels of backup power were carried out against occupant safety and comfort parameters to apply these metrics, with additional criteria imposed on grocery stores to capture the inventory and sales loss from food spoilage resulting from a lack of refrigeration power. Findings from this study demonstrate the feasibility of our proposed metrics and methodology to serve as a low-data burden means for stakeholders to evaluate the energy resilience of grocery stores, with greater implications in helping to understand the impact on community-scale energy resilience.

Siegel, Lino Sanchez↗

Marine and maritime uses

Activities related to: (1) understanding, controlling, and using the ocean's biological and physical processes for food and energy production and ship design purposes, and (2) providing navigation, communication, and data transmission technological aids which improve efficiency and enhance safety in maritime operations are disclosed.

Source record↗

Academy Sharing Knowledge (ASK). The NASA Source for Project Management Magazine, Volume 11, March 2003

APPL is a research-based organization that serves NASA program and project managers, as well as project teams, at every level of development. In 1997, APPL was created from an earlier program to underscore the importance that NASA places on project management and project teams through a wide variety of products and services, including knowledge sharing, classroom and online courses, career development guidance, performance support, university partnerships, and advanced technology tools. ASK Magazine grew out of APPL's Knowledge Sharing Initiative. The stories that appear in ASK are written by the 'best of the best' project managers, primarily from NASA, but also from other government agencies and industry. Contributors to this issue include: Teresa Bailey, a librarian at the Jet Propulsion Laboratory, Roy Malone, Deputy Director in the Safety and Mission Assurance (S&MA) Office at the NASA Marshall Space Flight Center (MSFC), W. Scott Cameron, Capital Systems Manager for the Food and Beverage Global Business Unit of Procter and Gamble, Ray Morgan, recent retiree as Vice President of AeroVironment, Inc., Marty Davis, Program Manager of the Geostationary Operational Environmental Satellite (GOES) at the NASA Goddard Space Flight Center (GSFC) in Greenbelt, Maryland, Todd Post, editor of ASK Magazine, and works for EduTech Ltd. in Silver Spring, Maryland, Dr. Owen Gadeken, professor of Engineering Management at the Defense Acquisition University, Ken Schwer, currently the Project Manager of Solar Dynamics Observatory, Dr. Edward Hoffmwan, Director of the NASA Academy of Program and Project Leadership, Frank Snow, a member of the NASA Explorer Program at Goddard Space Flight Center since 1992, Dr. Alexander Laufer, Editor-in-Chief of ASK Magazine and a member of the Advisory Board of the NASA Academy of Program and Project Leadership, Judy Stokley, presently Air Force Program Executive Officer for Weapons in Washington, D.C. and Terry Little, Director of the Kinetic Energy Boost Office of the Missile Defense Agency.

Source record↗

Design of a Jettison System For Space Transit Vehicles

Many options to re-use waste are currently being developed by NASA. These include combustion, compaction, torrefaction, and converting waste materials to an easily stored base polymer for future use. Human exploration missions require large amounts of supplies such as food, clothing and spare parts. A many-month journey to Mars will result in the generation of a substantial amount of problematic waste products. It is thought that this waste must be discarded to enable a Mars transit mission. The most cost-effective, reliable, and safest method to address this problem may be to simply jettison these materials from the spacecraft. The ability to jettison requires a multi-component integrated system design. Major components include a launcher, airlocks, trash bags, and tracking system. Depending upon mission requirements, a jettison dedicated airlock may be necessary. In other cases, the crew airlock might be all that’s needed. Examples of what to jettison include food waste, fecal containers, broken equipment, foam, expendables, hygiene products, health items, wipes, and clothing. The most important design factor is always crew safety. Other features to consider are loss of air due to the inefficiencies of vacuum pumps in the airlock, outgassing from the bags, and liquids condensing on the airlock that result in unwanted coatings. The launching system design must consider many different features: while in planetary orbit, a minimum exit velocity is necessary so the jettisoned material will not complete an orbit only to return and strike the ship. Also, for planetary protection issues, enabling waste to be sent to distant non-critical locations is highly desirable. When the space craft is in planetary transit to say, Mars, the jettison exit velocity should be low if the material is not released along the direction of travel. This is to minimize trajectory perturbations due to jettison-imposed torque on the spacecraft. Design considerations for the launcher include exit velocity, power consumption, volume and mass of material jettisoned, and frequency of jettison. Finally, the launcher must be able to handle many different types and shapes of waste products. This paper will discuss these design issues and give guidance to a pathway forward.

Steve Sepka↗