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At least 181 records · Page 10

Rapid Electrochemical Detection and Identification of Microbiological and Chemical Contaminants for Manned Spaceflight Project

Microbial control in the spacecraft environment is a daunting task, especially in the presence of human crew members. Currently, assessing the potential crew health risk associated with a microbial contamination event requires return of representative environmental samples that are analyzed in a ground-based laboratory. It is therefore not currently possible to quickly identify microbes during spaceflight. This project addresses the unmet need for spaceflight-compatible microbial identification technology. The electrochemical detection and identification platform is expected to provide a sensitive, specific, and rapid sample-to-answer capability for in-flight microbial monitoring that can distinguish between related microorganisms (pathogens and non-pathogens) as well as chemical contaminants. This will dramatically enhance our ability to monitor the spacecraft environment and the health risk to the crew. Further, the project is expected to eliminate the need for sample return while significantly reducing crew time required for detection of multiple targets. Initial work will focus on the optimization of bacterial detection and identification. The platform is designed to release nucleic acids (DNA and RNA) from microorganisms without the use of harmful chemicals. Bacterial DNA or RNA is captured by bacteria-specific probe molecules that are bound to a microelectrode, and that capture event can generate a small change in the electrical current (Lam, et al. 2012. Anal. Chem. 84(1): 21-5.). This current is measured, and a determination is made whether a given microbe is present in the sample analyzed. Chemical detection can be accomplished by directly applying a sample to the microelectrode and measuring the resulting current change. This rapid microbial and chemical detection device is designed to be a low-cost, low-power platform anticipated to be operated independently of an external power source, characteristics optimal for manned spaceflight and areas where power and computing resources are scarce.

Pierson, Duane↗

ResDesign: Resilient by Design Platform for CPS Assessment and Validation

ResDesign project has developed integrated capabilities to help cyber physical system modelers and analysts to evaluate vulnerabilities and resilience of such systems using co-simulation-based attack testbed, graph-based visualization and monitoring tool, and Bayesian optimization-based co-design capability. The project demonstrates a collection of attack scenarios and use cases in an integrated software environment.

97 MATHEMATICS AND COMPUTING↗

Biomedical Monitoring and Countermeasures Facility

The Space Station Freedom Program (SSFP) represents the transition within the US Space program from the 'heroic' era of space flight (characterized most vividly by the Mercury and Apollo programs) to an epoch characterized by routine access to the space environment. In this new era, the unique characteristics of the microgravity environment will enable new types of research activities, primarily in the life sciences, materials science, and biotechnology fields. In addition to its role as a'microgravity science laboratory,' Space Station Freedom (SSF) constitutes the operational platform on which the knowledge and skills needed to continue our exploration of space will be acquired. In the area of spacecraft operations, these skills include the ability to assemble, operate, and maintain large structures in space. In the area of crew operations, the potentially harmful effects of extended exposure to microgravity must be understood in order to keep the crew mission capable. To achieve this goal, the complex process of physiological deconditioning must be monitored, and countermeasures utilized as needed to keep the individual crew members within acceptable physiological limits. The countermeasures program under development for the SSF Program is titled the Biomedical Monitoring and Countermeasures (BMAC) program. As implied by the name, this activity has two primary products, a biomedical monitoring element and a countermeasures development effort. The program is a critical path element in the overall SSF Program, and should be considered an essential element of operations on board the space station. It is readily apparent that the capability to both protect and optimize the health and performance of the human operators on board SSF will be a critical element in the overall success of the SSFP. Previous experience within the Russian space program has demonstrated that the time required for countermeasures on extended missions can become a monumental operational burden. Therefore, one of the primary objectives of the countermeasures development activity will be to design and implement countermeasures which are significantly more effective than the existing generation. Other primary objectives include the following: to set health and human performance standards for all mission phases; to determine critical issues that affect performance or return to flight status; to develop and implement monitoring systems to follow health and performance status; and to understand risk, and balance the resource costs of countermeasures vs. the benefit gained.

Stewart, Donald F.↗

Annual Site Environmental Report: 2020 (ASER) for the SLAC National Accelerator Laboratory

To satisfy the requirements of the United States Department of Energy (DOE) SLAC Site Office approved Site Compliance Plan for DOE Order 231.1B (Change 1, 2012, “Environment, Safety and Health Reporting,” the Environment, Safety and Health Division (ES&H) of the SLAC National Accelerator Laboratory prepares an annual report describing its environmental programs and activities. This Annual Site Environmental Report: 2020 summarizes the SLAC National Accelerator Laboratory compliance with standards and requirements, describes the management and monitoring systems in place, and highlights significant accomplishments for the year.

54 ENVIRONMENTAL SCIENCES↗

Annual Site Environmental Report: 2019 (ASER) for the SLAC National Accelerator Laboratory

To satisfy the requirements of the United States Department of Energy (DOE) SLAC Site Office approved Site Compliance Plan for DOE Order 231.1B, “Environment, Safety and Health Reporting,” the Environment, Safety and Health Division (ES&H) of the SLAC National Accelerator Laboratory prepares an annual report describing its environmental programs and activities. This Annual Site Environmental Report: 2019 summarizes the SLAC National Accelerator Laboratory compliance with standards and requirements, describes the management and monitoring systems in place, and highlights significant accomplishments for the year.

54 ENVIRONMENTAL SCIENCES↗

Use of PZT's for adaptive control of Fabry-Perot etalon plate figure

A Fabry Perot etalon, consisting of two spaced and reflective glass flats, provides the mechanism by which high resolution spectroscopy may be performed over narrow spectral regions. Space based applications include direct measurements of Doppler shifts of airglow absorption and emission features and the Doppler broadening of spectral lines. The technique requires a high degree of parallelism between the two flats to be maintained through harsh launch conditions. Monitoring and adjusting the plate figure by illuminating the Fabry Perot interferometer with a suitable monochromatic source may be performed on orbit to actively control of the parallelism of the flats. This report describes the use of such a technique in a laboratory environment applied to a piezo-electric stack attached to the center of a Fabry Perot etalon.

Skinner, WIlbert↗

Hydrogen Leak Modeling for Development of Smart Distributed Monitoring Under Unintended Releases

Hydrogen is a versatile and clean energy carrier that can be produced from various renewable sources such as wind, solar, and hydropower. Hydrogen has the potential to play a crucial role in decarbonizing industrial processes that are currently reliant on fossil fuels and provide long-duration and/or seasonal energy storage to enable electricity decarbonization. Hydrogen can also be used as a fuel for fuel cell vehicles, providing a zero-emission alternative to traditional internal combustion engines. DOE launched the Hydrogen Energy Earthshot (Hydrogen Shot) in June 2021 to reduce the cost of clean hydrogen by 80% to $1 per 1 kilogram in 1 decade ("1 1 1"). While promising, Hydrogen is highly-flammable, and in the presence of oxygen, it can form explosive mixtures. . Therefore, understanding leak scenarios is essential to evaluate and mitigate the safety risks associated with potential hydrogen leaks. An increased understanding of leak behavior, and having tools to model leaks, can help assess how hydrogen would disperse in different environments, influencing emergency response plans and safety measures, and identify potential issues with materials and design systems that can withstand the challenges posed by hydrogen. Recently, researchers have attempted to study hydrogen leaks for development of risk management strategies. However, the focus has been on closed or semi-closed spaces like storage rooms, vehicles, garages, and fueling stations - all promising locations for future hydrogen infrastructure. In this presentation, the modeling environment extends the span of research further by modeling hydrogen leak in an outdoor, open space. We will present the key challenges with modeling hydrogen leaks in an uncontrollable environment, how they were handled, and how modeling results informed sensor selection and placement. A Hydrogen research facility at the National Renewable Energy Laboratory (NREL) was used as a case study to model hydrogen leaks. In the future, Hydrogen wide area detection methodologies will be developed and tested at this site to monitor for unintended and operational hydrogen releases. The data generated from modeling will be used to develop a predictive model to detect hydrogen leak location based on concentration measured by sensors in this open space. Furthermore, the facility was also chosen because controlled hydrogen releases can be performed. A computational fluid dynamics (CFD) based modeling approach was taken to model hydrogen leak. The full-scale hydrogen facility was modeled with a large ambient domain. The electrolyzer at the facility can produce a controlled release rate of 27 kg-H2/hr. Site-specific atmospheric and weather condition data such as wind direction, wind speed at various altitudes, and temperature were used as inputs to the model. To capture the variability of weather conditions, a subset of the weather conditions experienced during daytime hours without precipitation over the course of three months was generated; using established data clustering techniques, a total of 100 condition sets were chosen. The results show statistical distributions and ranges of hydrogen concentrations at locations throughout the domain. These distributions are compared to experimental data from a constant mass flow, controlled hydrogen release at the facility. The stochastic wind conditions of the release make direct validation difficult, therefore, statistical comparison approaches were used. Wind conditions are found to significantly impact the release behavior, including direction and concentration. Sensor selection and placement is proposed for the facility and is now based on release behavior predicted for the facility given its weather patterns; this is much more informed than without the modeling results. The methodology and analysis procedure can be translated to other facilities using modified geometries and site-specific weather conditions. Hydrogen holds great promise as a renewable energy fuel, but ensuring safety in its production, storage, and use is paramount. Studying potential leak scenarios in an open space will help develop sensors to detect hydrogen on a large spectrum of concentration and eventually build a smart distributed monitoring system.

CFD↗

Development and testing of a continuous maritime monitor for radionuclide aerosols

Monitoring airborne concentrations of radionuclide activity may provide a timely warning to sea-based assets to avoid contamination from a radioactive plume. The development and testing of an automated aerosol monitoring system that can capture and detect radioactive particulate from marine air is presented. A custom electrostatic precipitator (ESP) was designed to capture particulate onto a reusable collection media. The collection efficiency of the ESP system for radon progeny was determined to be ~23%. A conservative calculation of the minimum detectable concentration of 214 Bi was estimated as 0.3-8 Bq/m 3 . The system was demonstrated in continuous operation, without consumables and limited maintenance, in a marine environment at the PNNL campus in Sequim, Washington. In conclusion, a successful 2-month deployment indicates the feasibility of the approach for continuous maritime monitoring for radionuclide aerosols.

Moore, Michael E. [Pacific Northwest National Labo↗

Hurricanes and turbulent floods threaten arsenic-contaminated coastal soils and vulnerable communities

Coastal environments, particularly those adjacent to Superfund sites, are at increased risk of contaminant release during natural disasters, posing serious threats to nearby communities. To investigate this issue, we employed an advanced laboratory flood simulator to impose arsenic-contaminated sediments to turbulent flooding events. We further monitored changes in arsenic collocation and solid-phase speciation using advanced synchrotron radiation-based techniques to understand the impacts of flooding on arsenic mobility. Our results demonstrate that turbulent conditions significantly enhance the resuspension of arsenic-rich sediments, resulting in increased arsenic release into the water. This mobilization is driven by the erosion of the reduced sediments and the redox-mediated transformation and dissolution of Fe and Mn (oxyhydr) oxides, which promote the release of As(III). We found that arsenic speciation on resuspended particles is closely tied to shear stress, with As(V) prevailing at low stress and the more toxic As(III) dominating at higher stress levels. In the post-erosion phase, solid-phase As(III) decreased while dissolved As(III) increased, indicating ongoing desorption. The persistence of multiple arsenic species on resuspended particles marks them as potential long-range transport vectors. Thus, the environmental impact of flooding and sediment resuspension extends beyond the event itself, raising longer-term concerns for arsenic mobility. Our comprehensive geospatial analysis revealed substantial overlap between arsenic-contaminated soils and regions at high risk of flooding and hurricanes across the conterminous United States. This overlap disproportionately impacts economically disadvantaged and marginalized communities. Approximately 40 million Americans reside within 10 kilometers of these high-risk contaminated zones, with nearly 28 million exposed to hurricane threats and around 18 million vulnerable to flooding risks. Alarmingly, over 40% of those affected by hurricanes and 33% of those impacted by flooding belong to underrepresented minority and low-income populations. These findings highlight the urgent need for targeted mitigation strategies to protect public health and address environmental justice concerns.

54 ENVIRONMENTAL SCIENCES↗

An improved specimen preparation method for marine shallow gas-bearing sand sediments and its validations

Instabilities of shallow gas-charged seabed are potential geological hazards in ocean engineering. In practice, the conventional field sampling techniques failed to obtain undisturbed gas-bearing sediments from the seabed for laboratory mechanical testing because of sensitive gas exsolution and escape from sediments. However, preparation of representative remoulded gas-charged specimens is a challenging issue, because it is rather difficult to quantitatively control the gas content and obtain uniform distribution of gas bubbles within the specimen. Given the above problems, this work proposes a reliable approach to reconstitute the high-saturation specimen of gas-charged sediments in the laboratory by an improved multifunction integrated triaxial apparatus (MITA). This apparatus is developed based on an advanced stress path triaxial system by introducing a temperature-controlled system and a wave-monitoring system. The temperature-controlled system is used to accurately mimic the in situ environments of sediments in the seabed. The wave-monitoring system is utilized to identify exsolution point of free gas and examine the disturbance of gas to specimens during gas exsolution. The detailed procedure of gassy specimen preparation is introduced. Then, the quality of prepared specimens using our improved apparatus is validated by the high-resolution micro-X-ray computed tomography (μCT) scanning test, from which bubble occurrence and size distribution within the gassy sand specimen can be obtained; and preliminary mechanical tests on gassy sand specimens with various initial saturation degrees are performed. The proposed specimen preparation procedure succeeds in proving the postulated occurrence state of gas bubbles in coarse-grained sediments and accurately controlling the gas content.

58 GEOSCIENCES↗

Potential applications of microbial genomics in nuclear non-proliferation

As nuclear technology evolves in response to increased demand for diversification and decarbonization of the energy sector, new and innovative approaches are needed to effectively identify and deter the proliferation of nuclear arms, while ensuring safe development of global nuclear energy resources. Preventing the use of nuclear material and technology for unsanctioned development of nuclear weapons has been a long-standing challenge for the International Atomic Energy Agency and signatories of the Treaty on the Non-Proliferation of Nuclear Weapons. Environmental swipe sampling has proven to be an effective technique for characterizing clandestine proliferation activities within and around known locations of nuclear facilities and sites. However, limited tools and techniques exist for detecting nuclear proliferation in unknown locations beyond the boundaries of declared nuclear fuel cycle facilities, representing a critical gap in non-proliferation safeguards. Microbiomes, defined as “characteristic communities of microorganisms” found in specific habitats with distinct physical and chemical properties, can provide valuable information about the conditions and activities occurring in the surrounding environment. Microorganisms are known to inhabit radionuclide-contaminated sites, spent nuclear fuel storage pools, and cooling systems of water-cooled nuclear reactors, where they can cause radionuclide migration and corrosion of critical structures. Microbial transformation of radionuclides is a well-established process that has been documented in numerous field and laboratory studies. These studies helped to identify key bacterial taxa and microbially-mediated processes that directly and indirectly control the transformation, mobility, and fate of radionuclides in the environment. Expanding on this work, other studies have used microbial genomics integrated with machine learning models to successfully monitor and predict the occurrence of heavy metals, radionuclides, and other process wastes in the environment, indicating the potential role of nuclear activities in shaping microbial community structure and function. Results of this previous body of work suggest fundamental geochemical-microbial interactions occurring at nuclear fuel cycle facilities could give rise to microbiomes that are characteristic of nuclear activities. These microbiomes could provide valuable information for monitoring nuclear fuel cycle facilities, planning environmental sampling campaigns, and developing biosensor technology for the detection of undisclosed fuel cycle activities and proliferation concerns.

59 BASIC BIOLOGICAL SCIENCES↗

An OSI Architecture for the Deep Space Network

The flexibility and robustness of a monitor and control system are a direct result of the underlying inter-processor communications architecture. A new architecture for monitor & control at the Deep Space Network Communications Complexes has been developed based on the Open System Interconnection (OSI) standards. The suitability of OSI standards for DSN M&C has been proven in the laboratory. The laboratory success has resulted in choosing an OSI-based architecture for DSS-13 M&C. DSS-13 is the DSN experimental station and is not part of the "operational" DSN; it's role is to provide an environment to test new communications concepts can be tested and conduct unique science experiments. Therefore, DSS-13 must be robust enough to support operational activities, while also being flexible enough to enable experimentation. This paper describes the M&C architecture developed for DSS-13 and the results from system and operational testing.

OSI↗

Microstructural Evolution of Tantalum During Deformation and Subsequent Annealing

Microstructure-aware models are necessary to predict the behavior of material based on process knowledge or to extrapolate mechanical properties of materials to environmental conditions which are not easily reproduced in the laboratory, e.g., nuclear reactor environments. Elemental Ta provides a relatively simple BCC system in which to develop a microstructural understanding of deformation processes which can then be applied to more complicated BCC alloys. In situ neutron diffraction during compressive deformation and subsequent heat treatment have been used to monitor the evolution of microstructural features in Ta throughout simulated processing steps. Crystallographic texture and dislocation density are determined as a function of first plastic strain, then temperature. Lattice strains are determined and attributed to stresses at macroscopic, grain and dislocation length scales. The increase of the dislocation density through deformation and subsequent recovery during heat treatment is monitored through the changing diffraction line profile. Also, randomization of the texture is used as a signature of recrystallization. The recovery of dislocations through annihilation is not observed to depend on the initial dislocation density in the range studied here. In contrast, recrystallization is observed to depend strongly on the initially dislocation density.

36 MATERIALS SCIENCE↗

Los Alamos National Laboratory 2022 Annual Site Environmental Report (Rev. 2)

Los Alamos National Laboratory (Laboratory) annual site environmental reports are prepared each year by the Laboratory’s environmental organizations as required by U.S. Department of Energy Order 231.1B, Administrative Change 1, Environment, Safety, and Health Reporting, and Order 458.1, Administrative Change 4, Radiation Protection of the Public and the Environment. The chapters in this report discuss our compliance with environmental laws, regulations, and orders (Chapter 2, Compliance Summary); how we manage the Laboratory’s environmental performance and assure the quality of data from analysis of environmental samples (Chapter 3, Environmental Programs and Analytical Data Quality); how we monitor for air emissions of radioactive materials and for weather conditions (Chapter 4, Air Quality); how we monitor for effects of Laboratory operations on groundwater quality (Chapter 5, Groundwater Protection); how we monitor the levels of chemicals and radionuclides in storm water runoff and sediment (Chapter 6, Watershed Quality); how we monitor for the levels and effects of chemicals and radionuclides in plants, animals, soil, and vegetation (Chapter 7, Ecosystem Health); and finally, what radioactive dose or risk from chemical exposure that members of the public could experience as a result of Laboratory operations (Chapter 8, Public Dose and Risk Assessment).

54 ENVIRONMENTAL SCIENCES↗

Spectrograph Measures Contamination Of Optical Elements

Scanning-monochromator spectrograph designed to measure contamination on surfaces of optical elements as function of time. Repeatedly exposes samples to environment, then measures their transmittances or reflectances over range of wavelengths. Intended for use at vacuum-ultraviolet wavelengths to evaluate effects of outgassing, heating, and cooling on optical instruments. Principle of operation also applicable to spectral monitoring of time-dependent contamination at other wavelengths and in laboratory, industrial, or other settings.

Flint, Bruce K.↗

Single Primary Heat Extraction and Removal Emulator (SPHERE) Long Duration Testing

For the development of heat-pipe cooled microreactors, it is crucial to thoroughly understand the characteristics and functioning of heat pipes across a wide spectrum of operating conditions. Passive heat removal and its long-term performance stability are critical factors in this context. Enhanced experimental data is vital for evaluating the operational lifespan of alkali metal heat pipes. Idaho National Laboratory (INL) has successfully conducted an extended duration test on a high-performance sodium-filled heat pipe, closely monitoring the axial temperature profile, power supplied by the heaters, and heat removed by a gas-gap calorimeter. The results from this testing provide valuable data that are instrumental in supporting heat pipe validation efforts. Specifically, this data aids in the development and validation of Sockeye, the Multiphysics Object-Oriented Simulation Environment (MOOSE) tool under the US-DOE NEAMS program designed for heat pipe modeling. By comparing experimental results with Sockeye’s predictions, the tool's accuracy and reliability can be assessed and improved, thereby enhancing its capability to simulate heat pipe operations under various conditions.

22 - GENERAL STUDIES OF NUCLEAR REACTORS↗

Advanced Characterization Capabilities for Nuclear Materials via Nuclear Science User Facilities (NSUF)

Advanced post-irradiation examination (PIE) techniques are required to design new or improved nuclear materials, characterize, and understand in-core behavior of fuel and materials, and support the qualification of new reactor materials. The Nuclear Science User Facilities (NSUF) is the U.S. Department of Energy Office of Nuclear Energy's only designated nuclear science user facility. NSUF provides researchers access to PIE capabilities at Idaho National Laboratory and at a diverse mix of university, national laboratory and industrial partner institutions. The PIE capabilities include novel destructive and non-destructive techniques for radiation damage characterization, such as advanced diffraction techniques (X-ray, electron, or neutron) coupled to extreme environments; in-situ observation of microstructural evolution under irradiation; in-situ irradiation to monitor corrosive attack in coolant environments; in-situ irradiation and mechanical testing; and test methods for synergistic effects of superimposed extreme environments (temperature, irradiation, stress, corrosion) on materials behaviors. This talk will provide an overview of NSUF PIE capabilities.

36 MATERIALS SCIENCE↗

Low-Pressure Generator Makes Cleanrooms Cleaner

Scientists at NASA's Kennedy Space Center work in cleanrooms: laboratories with high degrees of cleanliness provided by strict control of particles such as dust, lint, or human skin. They are contaminant-free facilities, where the air is repeatedly filtered, and surfaces are smooth to prevent particles from getting lodged. Technicians working in these environments wear specially designed cleanroom "bunny suits" and booties over their street clothes, as well as gloves and face masks to avoid any contamination that may be imparted from the outside world. Even normal paper is not allowed in cleanrooms, only cleanroom low-particulate paper. These are sensitive environments where precision work, like the production of silicon chips or hard disk drives, is performed. Often in cleanrooms, positive air pressure is used to force particles outside of the isolated area. The air pressure in the Kennedy cleanrooms is monitored using high-accuracy, low-differential pressure transducers that require periodic calibration. Calibration of the transducers is a tricky business. In prior years, the analysis was performed by sending the transducers to the Kennedy Standards Laboratory, where a very expensive cross-floated, labor- intensive, dead-weight test was conducted. In the early 1990s, scientists at Kennedy determined to develop a technique and find equipment to perform qualification testing on new low-differential pressure transducers in an accurate, cost-effective manner onsite, without requiring an environmentally controlled room. They decided to use the highly accurate, cost-effective Setra Model C264 as the test transducer. For qualification testing of the Setra, though, a portable, lower-cost calibrator was needed that could control the differential pressure to a high degree of resolution and transfer the accuracy of the Standards Laboratory testing to the qualification testing. The researchers decided that, to generate the low-differential pressure setpoints needed for qualification testing, very small gas volume changes could be made against the test article, and a corresponding pressure change would be detected by a pressure standard. This allowed the researchers to recreate cleanroom air pressure settings without the use of a cleanroom. Thus was born the low-differential pressure generator. In 1993, a prototype was developed using a pair of PVC tanks, a volume controller, and a 1-pound-per-square-inch pressure standard. By 1995, the prototype was perfected into the unit that is still used today.

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