Field-grown miR156 transgenic switchgrass reproduction, yield, global gene expression analysis, and bioconfinement
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The Detector Control System (DCS) is one of the main pieces involved in the operation of the Compact Muon Solenoid (CMS) experiment at the LHC. The system is built using WinCC Open Architecture (WinCC OA) and the Joint Controls Project (JCOP) framework which was developed on top of WinCC at CERN. Following the JCOP paradigm, CMS has developed its own framework which is structured as a collection of more than 200 individual installable components each providing a different feature. Everyone of the systems that the CMS DCS consists of is created by installing a different set of these components. By automating this process, we are able to quickly and efficiently create new systems in production or recreate problematic ones, but also, to create development environments that are identical to the production ones. This latter one results in smoother development and integration processes, as the new/reworked components are developed and tested in production-like environments. Moreover, it allows the central DCS support team to easily reproduce systems that the users/developers report as being problematic, reducing the response time for bug fixing and improving the support quality.
Sandia National Laboratories (SNL) continued evaluation of total system performance assessment (TSPA) computing systems for the previously considered Yucca Mountain Project (YMP). This was done to maintain the operational readiness of the computing infrastructure (computer hardware and software) and knowledge capability for total system performance assessment (TSPA) type analysis, as directed by the National Nuclear Security Administration (NNSA), DOE 2010. This work is a continuation of the ongoing readiness evaluation reported in Lee and Hadgu (2014) and Hadgu et al. (2015). The TSPA computing hardware (CL2014) and storage system described in Hadgu et al. (2015) were used for the current analysis. One floating license of GoldSim with Versions 9.60.300, 10.5 and 11.1.6 was installed on the cluster head node, and its distributed processing capability was mapped on the cluster processors. Other supporting software were tested and installed to support the TSPA-type analysis on the server cluster. The current tasks included verification of the TSPA-LA uncertainty and sensitivity analyses, and preliminary upgrade of the TSPA-LA from Version 9.60.300 to the latest version 11.1. All the TSPA-LA uncertainty and sensitivity analyses modeling cases were successfully tested and verified for the model reproducibility on the upgraded 2014 server cluster (CL2014). The uncertainty and sensitivity analyses used TSPA-LA modeling cases output generated in FY15 based on GoldSim Version 9.60.300 documented in Hadgu et al. (2015). The model upgrade task successfully converted the Nominal Modeling case to GoldSim Version 11.1. Upgrade of the remaining of the modeling cases and distributed processing tasks will continue. The 2014 server cluster and supporting software systems are fully operational to support TSPA-LA type analysis.
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Mammalian pineal gland as neuroendocrine transducer, studying melatonin role in ovulation, gonadal growth, etc
Ulnar resonant frequency reproducibility as objective measure of skeletal status, discussing forearm and hand positioning effect
Judicious selection and maintenance of tape and tape transports minimizes dropouts.
Circuitry utilizing electrical feedback of instantaneous speaker coil velocity compensates for loudspeaker resonance, transient peaks and frequency drop-off so that sounds of widely varying frequencies and amplitudes can be reproduced accurately from high fidelity recordings of any variety.
The effects of monochromatic ultrasonic waves of 0.1, 0.5, 1.0, 2.0 and, 3.3 MHz frequency on the colony-forming ability of mammalian cells (M3-1,V79, Chang's and T-1) cultured in vitro have been studied to determine the nature of the action of ultrasonic energy on biological systems at the cellular level. The combined effect of ultrasound and X-rays has also been studied. It is concluded: (1) Ultrasonic irradiation causes both lethal and sublethal damage. (2) There is a threshold dose rate for lethal effects. (3) The effectiveness of ultrasonic waves in causing cell death probably depends on the frequency and the amplitude of the waves for a given cell line, indicating a possible resonance phenomenon.
Attempt has been made to separate mammalian male (Y) bearing sperm from female (X) bearing sperm. Both types of sperm are very dependent on gravity for their direction of movement. Proposed concept suggests electrophoretic force of suitable magnitude and direction may be effective means of separating X and Y sperm under zero gravity.
Software application or development in optical image digital data processing requires a fast, good quality, yet inexpensive hard copy of processed images. To achieve this, a Cambo camera with an f 2.8/150-mm Xenotar lens in a Copal shutter having a Graflok back for 4 x 5 Polaroid type 57 pack-film has been interfaced to an existing Adage, AGT-30/Electro-Mechanical Research, EMR 6050 graphic computer system. Time-lapse photography in conjunction with a log to linear voltage transformation has resulted in an interactive system capable of producing a hard copy in 54 sec. The interactive aspect of the system lies in a Tektronix 4002 graphic computer terminal and its associated hard copy unit.
Radiosensitivity of individual phases of the mitotic cycle was studied in synchronous cell cultures and in several biological objects. It was found that radiosensitivity changed essentially according to phases of the mitotic cycle, depending on the kind of cells, evaluation criteria and the radiation dosage. Tests on partially synchronized HeLa cell populations, according to the criterion of survival, showed them most sensitive during mitosis, as well as in later G sub 1- or early DNA-synthesizing stages. With radiation in doses of 300 rad, the proportion of surviving cells showed a sensitivity directly before DNA synthesis of approximately 4 times higher than the later S-phase and during the major portion of G sub 1- and G sub 2-periods. Sensitivity of cells in mitosis was approximately 3 times higher than in late G sub 1- and early S-phases.
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A schematic description of the steering control process is presented. It is shown that this process can be described in terms of levels of control. Level of control will depend on driver's skill in making use of 'clever' strategies which may be related to knowledge about the path to follow (input) and/or the vehicle under control. This knowledge may be referred to as an internal model of a particular task element. Internal information, as derived from these internal models will probably be used together with proprioceptive feedback. It is hypothesized that the efficiency of the higher levels of control will be dependent on the accuracy of both the internal and proprioceptive information. Based on this research philosophy a series of experiments is carried out. Two primary experiments were done in order to analyse subjects' ability to reproduce steering-wheel positions and movements without visual feedback. Steering-wheel angle amplitude, steering force and movement frequency were involved as independent variables.
The effects of test tube seals on the growth, flowering, and seed pod formation of Arabidopsis thaliana (L.) Heynh., mouse ear cress, and Cardamine oligosperma Nutt, bitter cress, are studied in order to assess the conditions used in weightlessness experiments. Among other results, it is found that the growth (height) and flowering (date of bud appearance) were suppressed in mouse ear cress in tubes sealed with Saran. Seed pod formation which occurred by day 45 in open-to-air controls, was still lacking in the sealed plants even up to day 124. The growth and flowering of bitter cress were also suppressed by the Saran seal, although up to day 55 the Saran-sealed plants were taller. It is suggested that atmospheric composition was the cause of the suppression of growth, flowering, and seed pod development in these plants, since the mouse ear cress renewed their growth and then set seed pods after the Saran seal was ruptured.
The effects of various levels of gravity force (obtained by rotation in clinostats or by centrifugation) and the near-weightlessness condition aboard orbiting spacecraft on the fertilization, embryonic development, maturation, and aging of animals are examined. Results obtained from the American and Soviet spaceborne biology experiments are presented including those on mammals, amphibians, fish, birds, invertebrates, and protozoa. Theoretical issues related to the effect of gravity on various physiological systems are discused together with the future research goals concerning human life in space. It is noted that life in space (after adaptation to near-weightlessness) might be significantly prolonged due to a reduction in metabolic rate and a concomitant decrease in oxygen radical reactions.
We report on an investigation of the trapezoidal design and fabrication defects in phase masks used to produce Bragg reflection gratings in optical fibers. We used a direct visualization technique to examine the nonuniformity of the interference patterns generated by several phase masks. Fringe patterns from the phase masks are compared with the analogous patterns resulting from two-beam interference. Atomic force microscope imaging of the actual phase gratings that give rise to anomalous fringe patterns is used to determine input parameters for a general theoretical model. Phase masks with pitches of 0.566 and 1.059 microns are modeled and investigated.