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At least 199 records · Page 11

"Way more than a donut and a PowerPoint": Women agricultural landowner learning circles and conservation outreach

Women’s conservation learning circles were designed to address the barriers facing women agricultural landowners, and have been shown to benefit these landowners in multiple ways. But how the resource professionals involved in the learning circle outreach method view the method is as of yet unknown. We use interview data from 35 resource professionals, including those from the Natural Resources Conservation Service and Soil and Water Conservation District to gain insight on their views of the learning circle outreach method. Our findings reveal some of the ways these resource professionals believe these meetings were successful, what they liked most, and what they believe could be improved. Overall, we identify how this outreach method has broad implications for resource professionals in helping work with groups where the traditional modes of conveying information (e.g., lecture formats and male-dominated meetings) are not well suited.

54 ENVIRONMENTAL SCIENCES↗

Whole-Genome Sequencing and Bioinformatic Analysis of Environmental, Agricultural, and Human Campylobacter jejuni Isolates From East Tennessee

As a leading cause of bacterial-derived gastroenteritis worldwide, Campylobacter jejuni has a significant impact on human health in both the developed and developing worlds. Despite its prevalence as a human pathogen, the source of these infections remains poorly understood due to the mutation frequency of the organism and past limitations of whole genome analysis. Recent advances in both whole genome sequencing and computational methods have allowed for the high-resolution analysis of intraspecies diversity, leading multiple groups to postulate that these approaches may be used to identify the sources of Campylobacter jejuni infection. To address this hypothesis, our group conducted a regionally and temporally restricted sampling of agricultural and environmental Campylobacter sources and compared isolated C. jejuni genomes to those that caused human infections in the same region during the same time period. Through a network analysis comparing genomes from various sources, we found that human C. jejuni isolates clustered with those isolated from cattle and chickens, indicating these as potential sources of human infection in the region.

59 BASIC BIOLOGICAL SCIENCES↗

Controlled-Environment Agriculture and the Geography of Food, Energy and Water Resilience in the United States

USDA guidelines call for an increase in fruit and vegetable consumption in U.S. households to promote healthier diets. Access to fresh fruits and vegetables are especially lacking in food deserts and food swamps (characterized by a prevalence of food that is highly processed and lacking in nutritional value). Adoption of the recommended healthy diet, which more than doubles the consumption of fruits and vegetables, would have a wide variety of health benefits and would reduce the land footprint of U.S diets. However, adopting a healthy diet would increase phosphate and nitrogen impacts associated with fertilizers and pesticides and would significantly increase freshwater consumption and energy use because of the resource intensity of field cultivation of fruits and vegetables. The majority of these crops are grown in just a few states, including California and Arizona, which are increasingly impacted by climate change. In addition, rural communities are rapidly becoming food deserts, while food produced in these areas is transported long distances to market. In recent years, highly intensified controlled environment (CE) agricultural systems, (i.e., vertical farming) have been developed to provide fresh food closer to consumers. Fruits and vegetables, many of which are amenable to CE culture, occupy a uniquely impactful segment of the food supply-chain, including their value in improving nutrition for vulnerable communities. The objective of this work is to elucidate the location dependency of the energy and water impacts of adoption of distributed CE farming for an important portion of the food system.

controlled environment agriculture↗

Power Analysis of an ePump Applied to the Linear Functions of an Agricultural Planter

Like many other industries, the agricultural industry has recently ex-perienced pressure to reduce vehicle emissions while improving productivity. Electric actuation is perceived as a viable solution to replace or augment hydrau-lic and mechanical actuation. However, electrification presents challenges with regards to linear functions, where hydraulic actuators have advantages in terms of compactness, tolerance to contamination and resistance to shocks. A combined electro-hydraulic actuation architecture can leverage the benefits of both electric and hydraulic actuation, while reducing the drawbacks of both approaches. This work investigates the potential of a centralized electric driven pump (ePump) system powering the pressure-controlled linear functions of an agricul-tural planter, with the goal of improving the operating point of the main supply pump. In this application, the rotary functions are hydraulically actuated, alt-hough such a solution could be applied also to electric rotary actuation. This is accomplished by setting the ePump to boost the pressure supplied by the tractor to the level required by the linear functions. An accumulator is used to stabilize the flow requirements of the linear functions, and control the pressure supplied to the actuators. Two control schemes are proposed for the regulation of the ac-cumulator pressure, one favoring an efficient operating point for the ePump, the other favoring stable steady state operation. A simulation model of the baseline system and proposed system is developed and validated using experimental data from a full-scale machine. Using the vali-dated simulation, both control architectures are then evaluated for improvement in system power consumption and dynamic requirements on the ePump to assess their effectiveness. Both systems demonstrate significant improvement in power consumption over the baseline system, with the best solution improving effi-ciency by 64%.

24 POWER TRANSMISSION AND DISTRIBUTION↗

Synchrotron resolved microscale mineralogy and elemental composition of individual plinthic and manganiferous nodules from agricultural soils

Iron and manganese oxides are strong sorbents and elemental scavengers in the environment that can form macroscopic nodules in soils. Despite their importance in the geochemical cycling of nutrients and contaminants, nodules are rarely characterized. When analyzed, they are often combined into bulk samples, obscuring individual characteristics. This study investigates the composition and mineralogy of individual plinthic and manganiferous nodules in soil from cropping systems in the Farming Systems Project in Beltsville, Maryland. Elemental composition indicates the accumulation of contaminants and nutrients, including copper in all nodules and high levels of phosphorus in select nodules. Sequential extractions determine that highly crystalline minerals comprise the nodules, locking away these nutrients and contaminants. Bulk X-ray diffraction (XRD) demonstrated distinct mineralogies in the plinthic and manganiferous nodules, with the former comprised of quartz, goethite, and kaolinite, and the latter consisting of quartz, Mn 2 O 3 , and hematite. Synchrotron and laboratory-based X-ray fluorescence (XRF) mapping gives critical insight into the elemental distribution within the nodules and elucidates spatial elemental associations, including the coupling of manganese and iron in the manganiferous nodules. XRF maps paired with µXRD provide microscale spatial resolution in mineralogy, which resolved several phases not apparent in bulk XRD, manganese’s association with goethite, and minerals indicative of redox transformations, including magnetite and maghemite. These novel results provide critical insight into the microscale mineralogy and individual elemental composition of plinthic and manganiferous nodules, as well as how they alter bulk soil geochemistry in ways largely unaccounted for in research on soil systems.

36 MATERIALS SCIENCE↗

Dredged sediments contain potentially beneficial microorganisms for agriculture and little harmful cyanobacteria

Soils worldwide are degrading, raising concerns about our ability to feed the growing global population. Soil amendments that can alleviate degradation are gaining attention. The application of sediments dredged from waterways to agricultural fields has increasing promise as a means for improving degraded soils. However, herbaceous plant species may have difficulty establishing on dredged material because of low nutrient availability, inhibitory levels of toxins, unsuitable moisture conditions and lack of microorganisms capable of ameliorating these characteristics. To counteract these issues, we sought to understand if the use of a cover crop would increase the abundance, diversity and function of beneficial soil microorganisms compared to harmful microorganisms in dredged sediments. We collected soil samples from two 100% dredged sediment plots, one where winter cereal rye (Secale cereal) was grown as a winter cover crop and one left fallow over the winter, followed by traditional corn (Zea mays) planting. We sampled both plots three times during the growing season: before cover crop application, following cover crop application but before corn planting and following final corn harvest. We then used high-throughput sequencing to identify the bacterial and fungal communities present in the samples. Our data show that cover crop application did not alter the microbial community in these plots. However, sampling time decreased species diversity and altered the composition of both fungal and bacterial communities recovered from these plots. Across both plots, microorganisms associated with carbon cycling were more abundant than those associated with harmful effects, including microcystin-producing cyanobacteria, which were an extremely small portion of the overall community. Our work suggests that dredged sediments have the potential to improve soil function through the addition of microorganisms associated with nutrient cycling, but a cover crop is not necessary to incur these benefits.

54 ENVIRONMENTAL SCIENCES↗

Multimodal, microspectroscopic speciation of legacy phosphorus in two US mid-Atlantic agricultural soils

To understand phosphorus (P) mobility in agricultural soils and its potential environmental risk, it is essential to directly measure solid phase P speciation. Often, bulk P K-edge X-ray absorption near edge structure (XANES) spectroscopy followed by linear combination fitting (LCF) is utilized to determine the solid P phases in soil. However, this method may limit results to only a few major phases. Additionally, XANES spectra for different P species may have very similar features, leading to an over- or underestimate of their contribution to LCF. Here, an improved P speciation by pairing multimodal microbeam-X-ray fluorescence (µ-XRF) mapping coupled with µ-XANES (microbeam-X-ray absorption near edge structure) analysis to directly speciate major and minor P phases on the micron scale is provided. We combined maps of both tender (P, sulfur, aluminum, and silicon) and hard energy (calcium, iron [Fe], and manganese) elements to evaluate the elemental co-locations with P. To better account for uncertainty assigning XANES peaks to individual compounds, a more quantitative fingerprinting by “spectral feature analysis” was completed. With this analysis, an R-factor is reported for the fit. These results were compared to traditional LCF. Pre-edge fitting results revealed the presence of a two-component pre-edge feature for phosphate adsorbed to ferrihydrite. Additionally, phytate co-precipitated with ferrihydrite (Phytate-Fe-Cop) had a pre-edge feature, indicating direct association with Fe. Lastly, a unique P species associated with manganese oxide was identified in the soil via multimodal mapping and µ-XANES. These results allow for better prediction of P dissolution and mobility.

36 MATERIALS SCIENCE↗

Landscape composition and configuration have scale-dependent effects on agricultural pest suppression

Increasing landscape heterogeneity (composition and configuration) can enhance natural enemy populations and support pest suppression in agricultural landscapes. Using a network-based data mining approach, we examined independent gradients of landscape composition and configuration at six spatial scales that were associated with pest suppression services measured at 32 sites in Michigan and Wisconsin, USA. We compared the relative effects of landscape composition and configuration across scales with those of local crop type (corn or grassland). We found that multiple gradients of configurational heterogeneity were independent of composition and strongly associated with pest suppression, with different configuration metrics being predictive of pest suppression depending on the spatial scales and regions considered. Landscapes that were more configurationally heterogeneous at smaller spatial scales consistently supported higher pest suppression. In Michigan, pest suppression increased in landscapes with high edge contrast between annual crops and surrounding habitats and high edge density of grassland within 250-500 m radii. In Wisconsin, pest suppression increased with large core area of grassland and high field density within a 250 m radius. The main compositional effect we found was a positive relationship between grassland cover and pest suppression occurring at larger spatial scales (1000-1500 m) and occurring in Wisconsin but not in Michigan. Our findings demonstrate that effects of landscape composition and configuration on pest suppression differ across spatial scales and vary regionally. The network-based data mining techniques used here could be useful for disentangling intercorrelated landscape metrics in a variety of other contexts in landscape ecology.

54 ENVIRONMENTAL SCIENCES↗

Bringing solar to agriculture: An interdisciplinary design and analysis of a Concord grape agrivoltaic system

Agrivoltaics presents an opportunity to integrate solar photovoltaics (PV) with agricultural production, but crop-specific challenges and operational constraints remain underexplored. This study develops and evaluates a Concord grape agrivoltaic system in the Lake Erie American Viticulture Area, where vineyards face economic pressures and land use conflicts with solar development. Starting with vertical, tracking, and overhead PV systems, we model photosynthetic photon flux density (PPFD) reduction in grapevines and power generation losses from shading using the Agrivoltaic Radiation Tool (ART). Based on these results, which indicate 0.47 % annual grapevine PPFD loss for vertical designs, 1.6 % for tracking, and up to 25 % for the overhead systems, the vertical and tracking designs are selected for further computational fluid dynamics (CFD) analysis to evaluate airflow interactions. CFD results show that vertical panels do not significantly impact airflow through the grapevine canopy, and that tracking systems in horizontal position may enhance airflow compared to a vine-only scenario. Considering operational constraints for tracking systems, the vertical design is selected for an economic evaluation to reveal key financial outcomes for solar developers (14-year payback period) and growers ($408 reduction in financial losses per acre annually). A sensitivity analysis quantifies uncertainty in power generation (±8.8 %) and PPFD (±5.0 %), ensuring model robustness across different vineyard conditions. Furthermore, these findings provide quantitative evidence for the feasibility of Concord grape agrivoltaics, demonstrating a synergistic opportunity for dual-use solar while preserving cultural heritage in grape-growing regions.

14 SOLAR ENERGY↗

Renewable ammonia for sustainable energy and agriculture: vision and systems engineering opportunities

Synthetic ammonia is essential for agriculture, but its production at present is unsustainable. Ammonia synthesized with hydrogen from renewable-powered electrolysis and nitrogen separated from air has the potential to alleviate these sustainability concerns while also having promise as a low-cost storage medium for intermittent renewable energy. This paper reviews recent research and development on the topic of renewable ammonia production and utilization as fertilizer and as energy storage. We describe our vision for synergistically combining these renewable ammonia applications to improve sustainability. Furthermore, we outline opportunities for systems engineering to play a crucial role in advancing the adoption of renewable ammonia in a manner which is sustainable, economically competitive, and reliable.

10 SYNTHETIC FUELS↗

Crop models: integrating systems from the molecular to global for agricultural productivity and sustainability

Mathematical models that simulate crop growth in response to environmental conditions and management practices are essential tools for exploring agriculture-based strategies to address food security and environmental sustainability challenges. Early applications of crop models focused on supporting farmers in making management decisions. Applications have since expanded to estimating future impacts on local and global food production from changing climates. Emerging applications of crop models aim to leverage how these models integrate plant processes across biological scales to identify engineering or breeding strategies that account for environmentally-responsive dynamics at field scales and for exploring solutions to improve sustainability. In this review, we highlight recent studies across these four broad application areas and highlight potential future directions for the crop modeling field.

Piao, Ximin [Univ. of Illinois at Urbana-Champaign↗

How to estimate soil organic carbon stocks of agricultural fields? perspectives using ex-ante evaluation

Estimating soil organic carbon (SOC) stocks of agricultural fields has a range of important applications from development of sustainable management practices to monitoring carbon stocks. There are many estimation strategies with the potential for more reliable estimates of SOC stock and more efficient use of soil sampling and analysis resources, especially by leveraging readily available auxiliary information such as remote sensing. However, concrete guidance for strategy selection is lacking. This study narrows this gap with a comparison of strategies for estimating deep SOC stock (0–60 cm) in a prototypical field. Using high density SOC stock measurements and simulation, we built on past studies by 1) ex-ante evaluating a large number of strategy options, 2) using a Bayesian approach to quantify the uncertainty of the comparison, and 3) considering multiple Bayesian models to assess sensitivity to this modeling choice. We found that, using readily available auxiliary information, both balanced and stratified sampling offer substantial improvements over simple random sampling. The auxiliary information most important for this improvement is a Sentinel-2 SOC index = blue / (green × red), followed by the topographic wetness index. We found that these results are robust to the choice of mapping method, but that there is uncertainty in the magnitude of improvement. Here, we recommend future studies implement this Bayesian approach for simulated ex-ante evaluation of SOC stock estimation strategies across more fields to investigate the generalizability of these findings.

54 ENVIRONMENTAL SCIENCES↗

Lignin-first biorefining of Nordic poplar to produce cellulose fibers could displace cotton production on agricultural lands

Here, we show that lignin-first biorefining of poplar can enable the production of dissolving cellulose pulp that can produce regenerated cellulose, which could substitute cotton. These results in turn indicate that agricultural land dedicated to cotton could be reclaimed for food production by extending poplar plantations to produce textile fibers. Based on climate-adapted poplar clones capable of growth on marginal lands in the Nordic region, we estimate an environmentally sustainable annual biomass production of ~11 tonnes/ha. At scale, lignin-first biorefining of this poplar could annually generate 2.4 tonnes/ha of dissolving pulp for textiles and 1.1 m 3 biofuels. Life cycle assessment indicates that, relative to cotton production, this approach could substantially reduce water consumption and identifies certain areas for further improvement. Overall, this work highlights a new value chain to reduce the environmental footprint of textiles, chemicals, and biofuels while enabling land reclamation and water savings from cotton back to food production.

09 BIOMASS FUELS↗

Microbiome engineering for sustainable agriculture: using synthetic biology to enhance nitrogen metabolism in plant-associated microbes

Plants benefit from symbiotic relationships with their microbiomes. Modifying these microbiomes to further promote plant growth and improve stress tolerance in crops is a promising strategy. However, such efforts have had limited success, perhaps because the original microbiomes quickly re-establish. Since the complex biological networks involved are little understood, progress through conventional means is time-consuming. Synthetic biology, with its practical successes in multiple industries, could speed up this research considerably. Some fascinating candidates for production by synthetic microbiomes are organic nitrogen metabolites and related pyridoxal-5'-phosphate-dependent enzymes, which have pivotal roles in microbe-microbe and plant-microbe interactions. This review summarizes recent studies of these metabolites and enzymes and discusses prospective synthetic biology platforms for sustainable agriculture.

59 BASIC BIOLOGICAL SCIENCES↗

Estimating soil N 2 O emissions induced by organic and inorganic fertilizer inputs using a Tier-2, regression-based meta-analytic approach for U.S. agricultural lands

Consistent methods are essential for generating country and region-specific estimates of greenhouse gas (GHG) emissions used for reporting and policymaking. The estimates of direct N 2 O emissions from U.S. agricultural soils have primarily relied on the use of emission factors (EFs, Tier-1) and process-based models (Tier-3). However, Tier-1 estimates are relatively crude while Tier-3 calculations can be costly. This work addressed this gap by developing a Tier-2, regression-based approach by leveraging a meta-database containing 1883 field N 2 O observations together with environmental and management covariates from 139 studies. Our results estimated higher monthly soil N 2 O emissions (N 2 O m , kg N/ha) during the growing season (0.38) than the fallow period (0.15), highlighting the importance of considering measurement periods when utilizing meta-databases for analyzing N 2 O drivers. Significantly different N 2 O m were found for tillage practices (conventional > no-till: 0.42 > 0.27), fertilizer type (liquid > solid manure: 0.55 > 0.32), and soil texture (fine > coarse: 0.36 > 0.22). The comparisons of the influence of crop type and rotation, water management, and soil order on N 2 O emissions are complicated by regional data availability and interactions among different factors. Additionally, the finding that N 2 O emissions reported based on area (N 2 O m ), N input rate (EF), or yield can alter treatment rankings underscores the need to establish transparent criteria for rewarding or discouraging regionally-based management practices using N 2 O metrics. Finally, we show how General Linear Models (GLMs) can be used to estimate country and regional Tier-2 N 2 O m using a suite of covariates. Our GLMs identified tillage, water management, N input type and rate, soil properties, and elevation as the most influential covariates for the conterminous U.S. The limited accuracy of regional-scale GLMs, however, suggests the need to further improve the quality and availability of GHG and covariate data through concerted efforts in data collection.

54 ENVIRONMENTAL SCIENCES↗

Molecular and Dual-Isotopic Profiling of the Microbial Controls on Nitrogen Leaching in Agricultural Soils under Managed Aquifer Recharge

Nitrate (NO 3 – ) leaching is a serious health and ecological concern in global agroecosystems, particularly those under the application of agricultural-managed aquifer recharge (Ag-MAR); however, there is an absence of information on microbial controls affecting NO 3 – leaching outcomes. We combine natural dual isotopes of NO 3 – ( 15 N/ 14 N and 18 O/ 16 O) with metagenomics, quantitative polymerase chain reaction (PCR), and a threshold indicator taxa analysis (TITAN) to investigate the activities, taxon profiles, and environmental controls of soil microbiome associated with NO 3 – leaching at different depths from Californian vineyards under Ag-MAR application. The isotopic signatures demonstrated a significant priming effect (P < 0.01) of Ag-MAR on denitrification activities in the topsoil (0–10 cm), with a 12–25-fold increase of 15 N–NO 3 – and 18 O–NO 3 – after the first 24 h of flooding, followed by a sharp decrease in the enrichment of both isotopes with ~80% decline in denitrification activities thereafter. In contrast, deeper soils (60–100 cm) showed minimal or no denitrification activities over the course of Ag-MAR application, thus resulting in 10–20-fold of residual NO 3 – being leached. Metagenomic profiling and laboratory microcosm demonstrated that both nitrifying and denitrifying groups, responsible for controlling NO 3 – leaching, decreased in abundance and potential activity rates with soil depth. TITAN suggested that Nitrosocosmicus and Bradyrhizobium, as the major nitrifier and denitrifier, had the highest and lowest tipping points with regard to the NO 3 – changes (P < 0.05), respectively. Overall, our study provides new insight into specific depth limitations of microbial controls on soil NO 3 – leaching in agroecosystems.

59 BASIC BIOLOGICAL SCIENCES↗

What Factors Drive the Changes in Water Withdrawals in the U.S. Agriculture and Food Manufacturing Industries between 1995 and 2010?

Climate change and increasing world population will directly impact the global food supply chain linkages. In the United States, agricultural production requires less irrigated water than before but it still accounts for a third of total water withdrawals. To better understand the evolution of its water use, we perform a structural decomposition analysis of water withdrawals across eight different crops and six livestock categories and differentiate the trends over 1995–2005 vs 2005–2010 to account for the role of the economic crisis in the second period. Based on USGS data, the results show that both periods experienced an overall decline in water withdrawals in the production of all crops except oilseeds. This trend is driven by a decrease in water intensity, reflecting greater efficiency of irrigation systems, and by reduced local per capita income in the second period. However, increased foreign demand for water-intensive sectors like oilseeds from NAFTA and Asian partners mitigated the decline. Results indicate also a decreasing water use in livestock production partially due to a shift from red to white meat consumption in the country. Arguably, recent tariff wars and border closures have greatly reduced the virtual water embodied in American exports.

29 ENERGY PLANNING, POLICY, AND ECONOMY↗

Printed Potentiometric Ammonium Sensors for Agriculture Applications

Ammonium (NH 4 + ) concentration is critical to both nutrient availability and nitrogen (N) loss in soil ecosystems but can be highly variable across spatial and temporal scales. For this reason, effectively informing agricultural practices such as fertilizer management and understanding of mechanisms of soil N loss require sensor technologies to monitor ammonium concentrations in real time. Our work investigates the performance of fully printed ammonium ion-selective sensors used in diverse soil environments. Ammonium sensors consisting of a printed ammonium ion-selective electrode and a printed Ag/AgCl reference were fabricated and characterized in aqueous solutions and three different soil types (sand, peat, and clay) under the range of ion concentrations likely to be present in soil (0.01–100 mM). The response of ammonium sensors was further evaluated under variable gravimetric moisture content in the soil to reflect their reliability under field conditions. Ammonium sensors demonstrated a sensitivity of 53.6 ± 5.1 mV/decade when tested in aqueous solution, and a sensitivity of 55.7 ± 11 mV/dec, 57.5 ± 4.1 mV/dec, and 43.7 ± 4 mV/dec was measured in sand, clay, and peat soils, respectively.

60 APPLIED LIFE SCIENCES↗