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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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At least 199 records · Page 11
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DOE OSTI2020

Carrier-assisted One-pot Sample Preparation for Targeted Proteomics Analysis of Small Numbers of Human Cells

Protein analysis of small numbers of human cells is primarily achieved by targeted proteomics with antibody-based immunoassays, which have inherent limitations (e.g., low multiplex and unavailability of antibodies for new proteins). Mass spectrometry (MS)-based targeted proteomics has emerged as an alternative because it is antibody-free, high multiplex, and has high specificity and quantitation accuracy. Recent advances in MS instrumentation make MS-based targeted proteomics possible for multiplexed quantification of highly abundant proteins in single cells. However, there is a technical challenge for effective processing of single cells with minimal sample loss for MS analysis. To address this issue, we have recently developed a convenient protein carrier-assisted one-pot sample preparation coupled with liquid chromatography (LC) - selected reaction monitoring (SRM) termed cLC-SRM for targeted proteomics analysis of small numbers of human cells. This method capitalizes on using the combined excessive exogenous protein as a carrier and low-volume one-pot processing to greatly reduce surface adsorption losses as well as high-specificity LC-SRM to effectively address the increased dynamic concentration range due to the addition of exogeneous carrier protein. Its utility has been demonstrated by accurate quantification of most moderately abundant proteins in small numbers of cells (e.g., 10-100 cells) and highly abundant proteins in single cells. The easy-to-implement features and no need for specific devices make this method readily accessible to most proteomics laboratories. In this study, we have provided a detailed protocol for cLC-SRM analysis of small numbers of human cells including cell sorting, cell lysis and digestion, LC-SRM analysis, and data analysis. Further improvements in detection sensitivity and sample throughput are needed towards targeted single-cell proteomics analysis. We anticipate that cLC-SRM will be broadly applied to biomedical research and systems biology with the potential of facilitating precision medicine.

60 APPLIED LIFE SCIENCES↗
NASA NTRS1971

Biomedical applications of aerospace technology

Aerospace technology transfer to biomedical research problems is discussed, including transfer innovations and potential applications. Statistical analysis of the transfer activities and impact is also presented.

Castles, T. R.↗
NASA NTRS1972

Life sciences payload definition and integration study. Volume 3: Appendices

Detail design information concerning payloads for biomedical research projects conducted during space missions is presented. Subjects discussed are: (1) equipment modules and equipment item lists, (2) weight and volume breakdown by payload and equipment units, (3) longitudinal floor arrangement configuration, and (4) nonbaseline second generation layouts.

Source record↗
NASA NTRS1974

Life sciences payload definition and integration study. Volume 2: Requirements, design, and planning studies for the carry-on laboratories

The task phase concerned with the requirements, design, and planning studies for the carry-on laboratory (COL) began with a definition of biomedical research areas and candidate research equipment, and then went on to develop conceptual layouts for COL which were each evaluated in order to arrive at a final conceptual design. Each step in this design/evaluation process concerned itself with man/systems integration research and hardware, and life support and protective systems research and equipment selection. COL integration studies were also conducted and include attention to electrical power and data management requirements, operational considerations, and shuttle/Spacelab interface specifications. A COL program schedule was compiled, and a cost analysis was finalized which takes into account work breakdown, annual funding, and cost reduction guidelines.

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NASA NTRS1976

Cardiovascular and pulmonary dynamics by quantitative imaging

The accuracy and range of studies on cardiovascular and pulmonary functions can be greatly facilitated if the motions of the underlying organ systems throughout individual cycles can be directly visualized and readily measured with minimum or preferably no effect on these motions. Achievement of this objective requires development of techniques for quantitative noninvasive or minimally invasive dynamic and stop-action imaging of the organ systems. A review of advances in dynamic quantitative imaging of moving organs reveals that the revolutionary value of cross-sectional and three-dimensional images produced by various types of radiant energy such as X-rays and gamma rays, positrons, electrons, protons, light, and ultrasound for clinical diagnostic and biomedical research applications is just beginning to be realized. The fabrication of a clinically useful cross-section reconstruction device with sensing capabilities for both anatomical structural composition and chemical composition may be possible and awaits future development.

Wood, E. H.↗
NASA NTRS1979

Vacuum-ultraviolet laser uses superfluid helium

Vacuum ultra violet laser in wavelength around 0.800 microns is produced by using optical pumping to increase lifetimes of excited metastable molecules in super fluid helium. In method, super fluid helium is pumped electronically to produce excited HE2, and then pumped by circularly polarized 0.9096 - micron radiation to aline excited HE2 molecular spins. High power ultraviolet radiation has potential applications in molecular reaction studies, power transmission in space, and biomedical research.

Zmuidzinas, J. S.↗
NASA NTRS1977

The use of nonhuman primates in space

Space related biomedical research involving nonhuman primates is reviewed. The scientific assets of various species and the instruments used for monitoring physiological processes during long duration experimentations are described.

Richard C. Simmonds↗
NASA NTRS1977

The squirrel monkey as a candidate for space flight

Because of its size and other unique diurnal-primate characteristics, the squirrel monkey is used in: (1) actual bioflight missions, (2) in laboratory tests designed to clarify the risks to man during launch and recovery as well as in hazardous spaceflight environments; and (3) in the acquisition of data on unknown risks encountered in long duration space exploration. Pertinent data concerning samiri sciureus as described in published and unpublished reports are summarized. Topics include: taxonomy, ethology, life history, sensory-learning-motor capabilities in primate perspective, anatomy and physiology (including homeostatic adaptation to stress), susceptibility to environmental hazards, reproduction, care and clinical management, and previous use in aerospace biomedical research.

Brizzee, K. R.↗
NASA NTRS1977

Depth-dose relations for heavy ion beams

Radiation transport of heavy ions in matter is of interest to radiological protection in space and high-altitude aircraft. In addition, heavy ion beams are expected to be of advantage in radiotherapy since their characteristic Bragg curve allows a relative reduction of the dose in reaching a tumor site and the near elimination of exposure beyond the tumor region as the beam exits the body. Furthermore, the radioresistance of tumorous cells due to their hypoxic state may be reduced or eliminated by the high specific ionization of heavy ion beams. The depth-dose distribution of heavy ion beams consists of energy deposited by the attenuated primary beam with its characteristic Bragg curve and a relatively unstructured background due to secondary radiations produced in nuclear reactions. As the ion mass increases, the secondary contribution becomes more structured and may add significantly to the Bragg peak of the primary ions. The result for heavy ions (z greater than 20) is a greatly broadened Bragg peak region, especially in comparison to straggling effects, which may prove to be of importance in radiotherapy and biomedical research.

Wilson, J. W.↗
NASA NTRS1981

A meeting with the universe: Science discoveries from the space program

A general history of space exploration is presented. The solar system is discussed. The Sun-Earth relationship is considered, including magnetic fields, solar wind, the magnetosphere, and the Sun-weather relationship. The universe beyond the solar system is discussed. Topics include stellar and galactic evolution, quasars and intergalactic space. The effects of weightlessness and ionizing radiation on human beings are considered. The possibility of extraterrestrial life is discussed. Lunar and planetary exploration, solar-terrestrial physics, astrophysics, biomedical research and exobiology are reviewed. Numerons color illustrations are included.

French, B. M.↗
NASA NTRS1983

Future thrusts in life sciences experimentation in space

Biomedical research objectives for future Shuttle and/or Space Station missions are discussed, with a focus on experiments exploring the physiological effects of microgravity. Experience up to the present is found to indicate that molecular-level processes are not much affected by the space environment, so that future experiments should concentrate on larger-scale phenomena. Areas considered include cardiovascular, respiratory, skeletal, and muscular physiology; metabolism; neurophysiology; and behavior. Radiation effects are seen as well as understood on the basis of ground-based data, with the possible exception of HZE-particle radiation. The need for carefully constructed ground research to prepare for space experiments is stressed.

Dietlein, L. F.↗
NASA NTRS1985

The issue is leadership

Four NASA Phase B centers (NASA-Johnson, NASA-Marshall, NASA-Goddard, and NASA-Lewis) are responsible for construction, assembly, servicing, habitat, and other particular tasks and functions of the Space Station. The project has been joined by the aerospace programs of Canada, Japan, and the European Space Agency, ensuring technological and financial support, and cooperative use by the participants. Some of the future uses of the Space Station include biomedical research and applications; experiments in solar-terrestrial physics and astronomy; building, maintenance, and launching of space instruments and planetary missions; manufacturing and processing of materials that call for the conditions of microgravity and weightlessness; supporting communication operations; and improving earth and atmospheric observations. The political significance of the Space Station as a symbol of leadership and of friendly cooperation is noted.

Beggs, J. M.↗
NASA NTRS1985

Production of Large-Particle-Size Monodisperse Latexes in Microgravity

A latex is a suspension of very tiny (micrometer-size) plastic spheres in water, stabilized by emulsifiers. The growth of billions of these tiny plastic spheres to sizes larger than can be grown on Earth is attempted while keeping all of them exactly the same size and perfectly spherical. Thus far on several of the Monodisperse Latex Reactor (MLR) flights, the latex spheres have been returned to Earth with standard deviations of better than 1.4%. In microgravity the absence of buoyancy effects has allowed growth of the balls up to 30 micrometers in diameter thus far. The MLR has now flown 5 times on the Shuttle. The MLR has now produced the first commercial space product; that is the first commercial material ever manufactured in space and marketed on Earth. Once it is demonstrated that these large-size-monodisperse latexes can be routinely produced in quantity and quality, they can be marketed for many types of scientific applications. They can be used in biomedical research for such things as drug carriers and tracers in the body, human and animal blood flow studies, membrane and pore-sizing in the body, and medical diagnostic tests.

Vanderhoff, J. W.↗
NASA NTRS1987

Liquid drop stability for protein crystal growth in microgravity

It is possible to grow protein crystals for biomedical research in microgravity by deploying a protein-rich solution from a syringe, forming a drop in which crystallization can occur with the proper degree of supersaturation. Drop stability is critical to the success of this research, due to the large drop sizes which can be achieved in space. In order to determine the type of syringe tips most suitable to support these large drops, tests were performed during brief periods of weightlessness onboard the NASA KC-135 low-gravity simulation aircraft. The drops were analyzed using three simple models in which the samples were approximated by modified pendulum and spring systems. It was concluded that the higher frequency systems were the most stable, indicating that of the syringes utilized, a disk-shaped configuration provided the most stable environment of low-gravity protein crystal growth.

Owen, Robert B.↗
NASA NTRS1989

Strategic implementation plan

The Life Science Division of the NASA Office of Space Science and Applications (OSSA) describes its plans for assuring the health, safety, and productivity of astronauts in space, and its plans for acquiring further fundamental scientific knowledge concerning space life sciences. This strategic implementation plan details OSSA's goals, objectives, and planned initiatives. The following areas of interest are identified: operational medicine; biomedical research; space biology; exobiology; biospheric research; controlled ecological life support; flight programs and advance technology development; the life sciences educational program; and earth benefits from space life sciences.

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NASA NTRS1991

Intramuscular Contact Lead Filled With Conductive Solution

Proposed sheath for braided-wire intramuscular conductor preserves electrical continuity even if wire breaks. Plastic sheath surrounds conductive solution in which braided wire immersed. At end of cable, wire and sheath crimped together and press-fit in porous titanium electrode. Implanted surgically with aid of device resembling catheter. Used to deliver electrical stimuli to muscles in biomedical research on human and animal physiology, development of prostheses, regeneration of nerves and muscles, and artificial implants.

Bamford, Robert M.↗
NASA NTRS1991

Compact X-Ray And Extreme-Ultraviolet Monochromator

Monochromator for x-ray and extreme-ultraviolet radiation provides higher spectral resolution than achieved with thin-metal-foil broad-band-pass filters. Uses Bragg reflection to band-pass-filter radiation from high-intensity, broad-spectrum source. Operates over much broader x-ray and extreme-ultraviolet spectrum than devices based upon natural crystals. Intended to filter continuum radiation from sources like synchrotrons, laser plasma sources, free-electron lasers, and wigglers to produce monochromatic beams for testing and analysis of x-ray and extreme-ultraviolet telescopes and microscopes, for calibration of photographic films and detectors, for biological and biomedical research, for x-ray lithography, for processing of materials, and for research in properties of materials.

Hoover, Richard B.↗
NASA NTRS1992

Multilayer Monochromator For Hard X Rays And Gamma Rays

Compact monochromator for hard x rays and gamma rays provides high spectral resolution with high throughput. Resembles instruments in "Compact X-Ray and Extreme-Ultraviolet Monochromator" (MFS-28499), "Scanning X-Ray or Extreme-Ultraviolet Monochromator" (MFS-28492), "Ultra-High-Spectral-Resolution X-Ray/EUV Monochromator" (MFS-28500), and "Four-Mirror X-Ray and Extreme-Ultraviolet Monochromator" (MFS-28498). Operates on principle of multilayer Bragg reflector. Used in nuclear, astronomical, and biomedical research, x-ray crystallography, research on processing materials, research in x-ray lasers, and x-ray lithography.

Hoover, Richard B.↗