Results for “CGI” — Page 11

NASA NTRSDate not supplied

Altered Immune Differentials Between Male and Female Mice Independent of Ionizing Irradiation

As upcoming exploratory missions to the Lunar surface and Mars during the Artemis Program will send both female and male crewmembers, studies identifying sex-specific effects are necessary. It is well-documented that the immune system engages different responses between females and males following exposures to infectious challenge, tumors surveillance, and autoimmune development. Yet, the immune effects of spaceflight-like exposures between the sexes are limited. Therefore, this study sought to address the effects of spaceflight-like conditions on immune differentials in female and male mice. We hypothesized that the spaceflight factor, ionizing radiation, will have sex-specific effects on immune differentials. To test this, singly-housed, 12-month old female and male wildtype (Wt) mice were exposed to acute ionizing gamma irradiation (50 cGy), followed by retro-orbital blood collections at 7-days post-irradiation. Leukocytes were analyzed by flow cytometry characterizing, granulocytes, monocytes, lymphocytes (cytotoxic/helper T cells and B cells), neutrophils, eosinophils, and NK/NKT cells. Briefly, minimal sex-specific effects were observed across the cell types listed at this time point, including changes in granulocyte and lymphocyte populations. The results revealed females displayed elevated granulocytes and reduced lymphocytes compared to their male counterparts, independent of irradiation exposure. These results suggest female mice may be more capable to produce innate inflammatory mediators but may be adaptively immune compromised. In brief, this study provides insight into the sex-specific immune risks following ionizing radiation that may be experienced during spaceflight, which is relevant for future countermeasure development.

spaceflight stressors↗

NASA NTRS2020

Let Dependent Low Dose and Synergistic Inhibition of Human Angiogenesis by Charged Particles: Validation of Micrornas That Drive Inhibition

The microvasculature permeates all tissues at the microscopic level making the whole body a target for Space Radiation. Depending on the trajectory a single heavy ion particle would traverse many micro-vessels as it passes through the body causing a widespread response. Studies on the effects of charged particles on the development of human 3D micro-vessel models show that angiogenesis is inhibited by all ions detectible at a dose of 1.25c Gy. However, there are two distinct mechanisms of inhibition depending on the linear energy transfer (LET). We show here that the inhibition of angiogenesis is demarcated into two groups. The blockage of the early motile stage of micro-vessel development occurs after exposure to light ions (LET 3 KeV/n or lower) whereas the prevention of the later stages of development when the microvessels widen into tubes occurs after exposure to heavy ions (LET 8 KeV/n or higher). Strikingly, the combined effect of each ion has a synergistic effect detectible as low as 0.6 cGy. The low fluence of these doses indicates a bystander effect where the response is transmitted to other cells by the secretion of molecules by targeted cells. Prime candidates are microRNAs (miRNAs) and we have previously identified three angiogenesis-associated miRNA regulators from serum, cardiac tissue, and micro-vessels exposed to a simplified Galactic Cosmic Radiation (GCR) simulation – a mixture of heavy and light ions found in Space. Synthetic molecules (Antagomirs) designed to inhibit the predicted miRNAs were successfully used to reverse the inhibition of angiogenesis by Space Radiation. With the simple application of the antagomirs as a countermeasure we have for the first time shown an amelioration of the complex response(s) of angiogenesis to Space Radiation. This opens a new avenue of possible mitigation strategies based on the regulation and understanding of master regulators. These results not only have impact with Space Radiation research, but also provide clues of possible therapeutics for terrestrial diseases in the clinical setting.

Yen-Ruh Wuu↗

NASA NTRSDate not supplied

Altered Immune Differentials Between Male and Female Mice Independent of Ionizing Irradiation

As upcoming exploratory missions to the Lunar surface and Mars during the Artemis Program will send both female and male crewmembers, studies identifying sex-specific effects are necessary. It is well-documented that the immune system engages different responses between females and males following exposures to infectious challenge, tumors surveillance, and autoimmune development. Yet, the immune effects of spaceflight-like exposures between the sexes are limited. Therefore, this study sought to address the effects of spaceflight-like conditions on immune differentials in female and male mice. We hypothesized that the spaceflight factor, ionizing radiation, will have sex-specific effects on immune differentials. To test this, singly-housed, 12-month old female and male wildtype (Wt) mice were exposed to acute ionizing gamma irradiation (50 cGy), followed by retro-orbital blood collections at 7-days post-irradiation. Leukocytes were analyzed by flow cytometry characterizing, granulocytes, monocytes, lymphocytes (cytotoxic/helper T cells and B cells), neutrophils, eosinophils, and NK/NKT cells. Briefly, minimal sex-specific effects were observed across the cell types listed at this time point, including changes in granulocyte and lymphocyte populations. The results revealed females displayed elevated granulocytes and reduced lymphocytes compared to their male counterparts, independent of irradiation exposure. These results suggest female mice may be more capable to produce innate inflammatory mediators but may be adaptively immune compromised. In brief, this study provides insight into the sex-specific immune risks following ionizing radiation that may be experienced during spaceflight, which is relevant for future countermeasure development.

spaceflight stressors↗

NASA NTRS2018

Fast Linearized Coronagraph Optimizer (FALCO) II: Optical Model Validation and Time Savings over Other Methods

We have developed the Fast Linearized Coronagraph Optimizer (FALCO), a new software toolbox for high-contrast, coronagraphic wavefront sensing and control. FALCO rapidly calculates the linearized deformable mirror (DM) response matrices, also called control Jacobians, and can be used for the design, simulation, or testbed operation of several types of coronagraphs. In this paper, we demonstrate that the optical propagation used in FALCO is accurate and matches PROPER. In addition, we demonstrate the drastic reduction in runtime when using FALCO for DM Jacobian calculations instead of the conventional method used, for example with a model of the Wide-Field Infrared Survey Telescope (WFIRST) Coronagraph Instrument (CGI). We then compare the relative accuracy between optical models in FALCO and PROPER.

Coker, Carl↗

NASA NTRS2018

Numerically Optimized Coronagraph Designs for the Habitable Exoplanet Imaging Mission (HabEx) Concept

The primary science goal of the Habitable Exoplanet Imaging Mission (HabEx), one of four candidate ﬂagship missions under investigation, is to image and spectrally characterize Earth-like exoplanets. It is well known that pupil obscurations degrade coronagraphic performance and complicate coronagraph design, so HabEx is planned to have an oﬀ-axis, unobscured primary mirror. We utilize the circular symmetry of the aperture to investigate 1D-radial coronagraph optimization methods that are prohibitively time-consuming or intractable in 2D, such as diﬀractive pupil remapping and concurrent, multi-plane optimization. We also directly constrain sensitivities to dynamic, low-order Zernike aberrations, which are separable in polar coordinates and can thus be propagated as 1D-radial integrals. The mask technologies in our designs claim heritage from the extensive modeling and testbed experiments performed by the Wide-Field Infrared Survey Telescope (WFIRST) Coronagraph Instrument (CGI) project. In this paper, we detail our optimization methods and outline future work to complete our design survey.

Balasubramaian, Kunjithapatham↗

NASA NTRS2019

WFIRST Low Order WaveFront Sensing and Control (LOWFS/C) performance on line-of-sight disturbances from multiple reaction wheels

In this paper we will describe the LOWFS/C LoS performance test in which the injected LoS disturbances are derived from all six reaction wheels on WFIRST, each with an independent varying wheel speed profile modeled for a typical coronagraph observational scenario. We will describe the LoS disturbances profile and testbed implementation approach. We will present the LOWFS/C LoS feedback and feed forward loops testbed performance under these realistic LoS disturbances in which we have demonstrated the LoS control that meets the CGI requirement.

Shelton, Chris↗

NASA NTRSDate not supplied

Root Growth Response of Arabidopsis Thaliana Seeds Exposed to Simulated Solar Particle Event Radiation and Microgravity

As NASA manned missions are planned to go past earth’s orbit, the effects of microgravity and radiation on plants will be essential to know as plants will be a part of the crew’s diet. APPROACH: Imbibed Arabidopsis thaliana seeds were exposed to simulated solar particle event (SPE) radiation, experiencing up to 80 cGy of radiation. After the radiation exposure, they were grown under simulated microgravity using a Random Positioning Machine. Images of the plants were taken after six to seven days of growth and the root length, top angle, tip angle were analyzed.

Karen Perkins↗

NASA NTRSDate not supplied

Prism Assembly for Roman Space Telescope Wide Field Instrument Slit-less Spectroscopy

The Roman Space Telescope (RST) is a three mirror anastigmat design with a 2.4 m primary mirror. RST will be based in L2 orbit, from where it will provide science information on exoplanets and dark energy using the Coronagraph Instrument (CGI) and Wide Field Instrument (WFI). The WFI features a 300 megapixel near-infrared detector array which provides a field of view 100 times larger than that of Hubble Space Telescope. The Prism Assembly is a small add-on instrument to the Roman Space Telescope’s Wide Field Instrument (WFI), installed in a slot in WFI’s element wheel. The Prism assembly has a passband from 0.75 µm to 1.8 µm, enabling a survey of redshifts in the range 0.2 to 1.7 µm. It provides low resolution slit-less spectroscopy with a spectral resolution R > 70 for all wavelengths, and R < 170 for λ > 0.8 µm across the full field. The Prism Assembly has the potential to provide more supernova spectra than ground-based spectroscopy during the mission lifetime. Being a late add-on to the RST mission, the Prism Assembly has had restrictions on size, weight and geometry, and a challenging schedule, which has dictated much of the implementation. Despite these challenges, the Prism Assembly is a pocket-sized high-performance spectrographic element, implemented as a refractive, all-spherical optical design using only two elements, one S-TIH-1 glass and one CaF2. This presentation will give an overview of the Prism Assembly, from design and implementation, through alignment, test and calibration. (100 words): The Roman Space Telescope is a three mirror anastigmat design with a 2.4 m primary mirror, which will provide science information on dark energy and exoplanets. The Prism Assembly is a small add-on instrument to the telescope’s Wide Field Instrument, enabling low resolution slit-less spectroscopy with a spectral resolution 70 < R < 170 across the full field from λ= 0.75 µm to 1.8 µm. The Prism Assembly is a refractive, all-spherical optical design using a glass and a CaF2 element. This presentation will give an overview of the Prism Assembly, from design and implementation, through alignment, test and calibration.

Space Instrumentation↗

NASA NTRSDate not supplied

Modified Cascading Generalized Inverse Control Allocation

The current aviation revolution towards electric propulsion aircraft (e.g., electric vertical takeoff-and-landing) brings unique control challenges. These vehicles are typically over-actuated (more effectors than desired control outcomes), may require control strategies for the three phases of flight (hover, transition and cruise), and currently have limited electric power availability. These vehicle challenges bring the need for optimal control allocation to the forefront of research. A leading control allocation algorithm, used in current flight vehicles, is the Cascading Generalized Inverse (CGI). Unfortunately, the Cascading Generalized Inverse algorithm is unable to achieve some desired outcomes, it intermittently provides non-optimal allocations, and it may fail to preserve moment direction near maximal achievable outcomes. In this research, the shortcomings of the Cascading Generalized Inverse algorithm are addressed by augmenting the algorithm with Scalar Difference Quadratic unsaturation identification and location at each iteration. Rigorous theory is shown that the Modified Cascading Generalized Inverse performs better at obtaining optimal allocations for all attainable outcomes. Numerical case studies for over-actuated vehicles demonstrate resolution to the aforementioned deficiencies.

Control Allocation↗

NASA NTRSDate not supplied

Modified Cascading Generalized Inverse Control Allocation

The current aviation revolution towards electric propulsion aircraft (e.g., electric vertical takeoff-and-landing) brings unique control challenges. These vehicles are typically over-actuated (more effectors than desired control outcomes), may require control strategies for the three phases of flight (hover, transition and cruise), and currently have limited electric power availability. These vehicle challenges bring the need for optimal control allocation to the forefront of research. A leading control allocation algorithm, used in current flight vehicles, is the Cascading Generalized Inverse (CGI). Unfortunately, the Cascading Generalized Inverse algorithm is unable to achieve some desired outcomes, it intermittently provides non-optimal allocations, and it may fail to preserve moment direction near maximal achievable outcomes. In this research, the shortcomings of the Cascading Generalized Inverse algorithm are addressed by augmenting the algorithm with Scalar Difference Quadratic unsaturation identification and location at each iteration. Rigorous theory is shown that the Modified Cascading Generalized Inverse performs better at obtaining optimal allocations for all attainable outcomes. Numerical case studies for over-actuated vehicles demonstrate resolution to the aforementioned deficiencies.

Control Allocation↗

NASA NTRS2021

Enabling High-Contrast Imaging of Binary Stars With the Roman Space Telescope Coronagraph Instrument

A large fraction of Sun-like stars is contained in binary systems, in fact within 10 pc there are 67 FGK stars, out of which 42 belong to a multi-star system, and 28 have companion leak that is greater than 1e-9 contrast, assuming typical Hubble-quality space optics. Currently, those binary stars are not included in the Roman Space Telescope Coronagraph Instrument target list, but they could be observed if high-contrast imaging around binary star systems using Roman was possible. The Multi-Star Wavefront Control (MSWC) algorithm can be used to suppress companion star leakage. If the targets have angular separations larger than the Nyquist controllable region of the Deformable Mirror, MSWC must operate in its Super-Nyquist (SN) mode. This mode requires a target star replica within the SN region in order to provide the ability to null speckles at SN angular separations. For the case of Roman, more than half of the targets that can be observed using MSWC, including Alpha Centauri, have angular separations larger than the Nyquist controllable region of the 48x48 actuator Deformable Mirror (DM) to be used. In this paper, we describe how super-Nyquist MSWC can be implemented in Roman CGI adding a mild grating on a replica of the Wide-field Shaped Pupil mask, which would use a spare space in the Shaped Pupil Active Mechanism. We present the feasibility analysis, simulation results, and discuss the latest results on MSWC experiment from three different laboratories at NASA Ames, the Jet Propulsion Laboratory, and the Subaru telescope.

Prada, Camilo Mejia↗

NASA NTRSDate not supplied

The Roman Space Telescope Optical System: Status, Test, and Verification

The Nancy Grace Roman Space Telescope (“Roman”) was prioritized by the 2010 Decadal Survey in Astronomy & Astrophysics and is NASA’s next flagship observatory. Launching no earlier than 2026, Roman will explore the nature of dark energy, as well as expand the census of exoplanets in our galaxy via microlensing. Roman will also demonstrate key technology needed to image and spectrally characterize extra-solar planets. Roman’s large field of view, agile survey capabilities, and excellent stability enable these scientific objectives, yet present unique challenges for the design, test, and verification of its optical system. The Roman optical system comprises an optical telescope assembly (OTA) and two instruments: the primary science wide-field instrument (WFI) and a technology demonstration coronagraph instrument (CGI), and the instrument carrier (IC), which meters the OTA to each instrument. This paper presents a status of the optical system hardware as it begins integration and test (I&T), as well as describes key optical test, alignment, and verification activities as part of the I&T program.

space telescope↗

NASA NTRSDate not supplied

Effects Of Five-Ion Galactic Cosmic Radiation Simulation On Immune Function, Brain, And Behavior In Male And Female Mice

Exposure to galactic cosmic radiation is a principal consideration of spaceflight missions, and with upcoming missions to the Moon and Mars, it is increasingly imperative to elucidate the effects of space travel beyond the lower Earth orbit. Additionally, with the first female astronaut to soon travel to the Moon there is a strong need to understand the biological sex differences to adaptation to the deep space environment. While the effects of spaceflight on the nervous system are not fully known, studies in animal models have shown that exposure to ionizing radiation can cause neuronal damage and lead to downstream cognitive and behavioral deficits. To simulate the type of radiation exposure occurring during spaceflight, model organisms can be exposed to relevant doses via Five-Ion Galactic Cosmic Radiation Simulation at the NASA National Space Radiation Laboratory at Brookhaven National Laboratory. We have investigated the neurobehavioral responses to space environment-like radiation exposure. Male and female 23–24-week-old mice (age-matched to average astronaut age) were exposed to 5, 15 and 50 cGy. Following exposure, immune, brain and behavioral (sensorimotor, risk-taking and cognitive) measures were acquired at ‘Acute’ (IR+24hrs, IR+72hrs), ‘Intermediate’ (IR+14 days) and ‘Delayed’ (IR+28 to IR+124 days) to inform biological responses anticipated during a transit to Moon and Mars. There were pronounced sex differences observed in all outcome measurements, while very few radiation induced effects were observed. Those dose effects that were observed were primarily in cytokine expression and less so in behavioral measurements. Further studies will investigate if radiation, microgravity and social isolation combine synergistically to trigger an oxidative stress response that alters immune homeostasis, brain structure/function, and neurobehavioral/cognitive performance, ultimately to characterize risks and identify appropriate countermeasures in both women and men in anticipation of future deep space missions.

Stephanie Puukila↗

NASA NTRS2024

Long-term effects of acute irradiation and isolation on crew age-matched mice

The future of space missions beyond low-Earth orbit presents exciting opportunities but may come with formidable health challenges. Astronauts face a unique combination of space stressors capable of exerting effects on the central nervous system (CNS) with possible sex-dependent differences. The interaction of these combined spaceflight stressors may induce oxidative stress, thereby altering brain integrity and behavioral performance. Understanding these changes is critical for safeguarding astronaut health and enabling successful exploration. By understanding the physiological responses to the combined effects of ionizing radiation (IR) and social isolation in the brain through immunohistochemistry (IHC), proteomics and cytokine expression analyses, we aim to identify CNS pathways ripe for countermeasure development to mitigate adverse brain changes with future deep space exploration. Here, we study the combined effects of simulated 5-ion galactic cosmic radiation (GCRsim) at acute dosage (5, 15, and 50 cGy) and social isolation on crew age-matched male and female mice at 124 days post-irradiation. We conducted analyses of hippocampal cytokine biomarkers and proteomic profiling on hippocampal lysates. Our findings reveal sex-specific regulation of hippocampal cytokines and proteomic profiles. Ongoing immunohistochemistry findings on the hippocampus will provide qualitative information regarding key protein expression and cellular changes such as microglial activation, blood brain barrier integrity, and neuroinflammation. With future plans for long-duration space missions, such as Mars exploration, understanding CNS changes over extended periods in mice can provide insights into potential long-term health impacts on astronauts. This project will provide us with valuable insights into sex-differences in neurobiological processes and disease mechanisms impacting men and women in space.

space radiation↗