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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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At least 199 records · Page 11
All sourcesNASA NTRSDOE OSTI
DOE OSTI2025

Nanodiffraction imaging of ionically driven phase separation in cobaltite heterostructures

Controlling the anionic stoichiometry and distribution of perovskite cobaltites presents a powerful method for tailoring the magnetic and electronic properties for magneto-ionic applications. Nevertheless, the influence of nanoscale morphology, including phase separation and defects in cobaltite heterostructures, remains largely unexplored. We used x-ray nanodiffraction to directly image the nanoscale topotactic phase transformation in La 1−x Sr x CoO 3 (LSCO) heterostructures capped with a reactive aluminum (Al) layer (i.e., oxygen “getter”) of varying thicknesses (0.5, 3, and 7 nm). Nanodiffraction images reveal the formation of highly elongated BM filaments extending over mesoscopic lengths (>67 μm), indicating a strong tendency toward oxygen vacancy ordering in both P and BM phases in all heterostructures. Local strain and strain gradient analysis shows a strong correlation between the depletion of the P phase and strain gradients. Our measurements suggest that elongation of the BM filaments is the predominant mechanism for topotactic transformation in LSCO/Al heterostructures, preferred over coarsening, branching, and fractal growth. Our study provides a detailed nanoscale analysis of ionically driven phase transitions in LSCO/Al heterostructures, which is crucial for tailoring the functional properties of cobaltites for magneto-ionic devices.

36 MATERIALS SCIENCE↗
DOE OSTI2026

Studies of localized mix and asymmetry caused by the stalk in inertial fusion experiments at OMEGA

In an inertially confined fusion experiment, a capsule of fusion fuel is compressed to high temperatures and densities using lasers, creating a hot plasma in which thermonuclear fusion reactions take place. To achieve efficient conversion of laser energy to fuel heating, the implosion must be as spherically symmetric as possible. One common source of asymmetry that degrades performance is the stalk and glue spot that hold the capsule in place before the experiment. The exact nature and magnitude of this perturbation had not been previously quantified. In this work, inertially confined fusion experiments were executed with an additional stalk on the capsule to measure and quantify the effect of the stalk and glue spot on the symmetry and performance. It is found that the stalk drives low-mode asymmetry by shadowing the laser drive on the part of the shell near the glue spot, enhancing the hot-spot velocity toward the stalk by 40–80 km/s and elongating the hot-spot along the stalk axis. It is also found that localized mix due to the additional stalk has a negligible effect on implosion performance. Two-dimensional radiation-hydrodynamic simulations using the xRAGE code reproduce the direction of the experimentally observed change to hot-spot velocity and implosion performance metrics, but not the observed elongation, suggesting that this feature results from an interaction between stalk perturbations and other preexisting asymmetry seeds, such as laser drive asymmetry.

70 PLASMA PHYSICS AND FUSION TECHNOLOGY↗
DOE OSTI2022

RNA polymerase II trapped on a molecular treadmill: Structural basis of persistent transcriptional arrest by a minor groove DNA binder

Elongating RNA polymerase II (Pol II) can be paused or arrested by a variety of obstacles. These obstacles include DNA lesions, DNA-binding proteins, and small molecules. Hairpin pyrrole-imidazole (Py-Im) polyamides bind to the minor groove of DNA in a sequence-specific manner and induce strong transcriptional arrest. Remarkably, this Py-Im–induced Pol II transcriptional arrest is persistent and cannot be rescued by transcription factor TFIIS. In contrast, TFIIS can effectively rescue the transcriptional arrest induced by a nucleosome barrier. The structural basis of Py-Im–induced transcriptional arrest and why TFIIS cannot rescue this arrest remain elusive. Here we determined the X-ray crystal structures of four distinct Pol II elongation complexes (Pol II ECs) in complex with hairpin Py-Im polyamides as well as of the hairpin Py-Im polyamides–dsDNA complex. We observed that the Py-Im oligomer directly interacts with RNA Pol II residues, introduces compression of the downstream DNA duplex, prevents Pol II forward translocation, and induces Pol II backtracking. These results, together with biochemical studies, provide structural insight into the molecular mechanism by which Py-Im blocks transcription. Our structural study reveals why TFIIS fails to promote Pol II bypass of Py-Im–induced transcriptional arrest.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2023

Operational space and performance limiting events in the first physics campaign of MAST-U

Abstract The MAST-U fusion plasma research device, an upgrade to the Mega Amp Spherical Tokamak, has recently completed its first campaign of physics operation. MAST-U operated with Ohmic, or one or two neutral beams for heating, at 400–800 kA plasma current, in conventional or ‘SuperX’ divertor configurations. Equilibrium reconstructions provide key plasma physics parameters vs. time for each discharge, and diagrams are produced which show where the prevalence of operation occurs as well as the limits in various operational spaces. When compared to stability limits, the operation of MAST-U so far has generally stayed out of the low q , low density instability region, and below the high density Greenwald limit, high beta global stability limit, and high elongation vertical stability limit. MAST-U still has the potential to reach higher elongation, which could benefit the plasma performance. Despite the majority of operations happening below established stability limits, disruptions do occur in the flat-top phase of MAST-U plasmas. The reasons for these disruptions are highlighted, and possible strategies to avoid them and to extend the operational space of MAST-U in future campaigns are discussed.

70 PLASMA PHYSICS AND FUSION TECHNOLOGY↗
DOE OSTI2023

Initial Fulcher band observations from high resolution spectroscopy in the MAST-U divertor

High resolution D$^*_2$ Fulcher band spectroscopy was used in the MAST-U divertors during Super-X and (shorter-legged) elongated divertor density ramps with D 2 fuelling from the mid-plane high-field side. In the Super-X case, the upper divertor showed ground state rotational temperatures of the D 2 molecules increasing from ~6000 K, starting at the detachment onset, to ~9000 K during deepening detachment. This was correlated with the movement of the Fulcher emission region towards the X-point, which is in turn correlated with the movement of the ionisation source. The increase in rotational temperature occurred throughout the divertor except near the divertor entrance, where ionisation was still the dominant process. Qualitative agreement was obtained between the lower and upper divertor. Similar rotational temperatures were obtained in the elongated divertor before the detachment onset, although the increase in rotational temperature during detachment was less clearly observed as less deep detachment was obtained. The measured vibrational distribution of the upper Fulcher state does not agree with a ground state Boltzmann distribution but shows a characteristically elevated population in the ν = 2 and ν = 3 bands in particular; which is strongly correlated to the rotational temperature.

70 PLASMA PHYSICS AND FUSION TECHNOLOGY↗
DOE OSTI2024

Assessment of vertical stability for negative triangularity pilot plants

Abstract Negative triangularity (NT) tokamak configurations may be more susceptible to magneto-hydrodynamic instability, posing challenges for recent reactor designs centered around their favorable properties, such as improved confinement and operation free of edge-localized modes. In this work, we assess the vertical stability of plasmas with NT shaping and develop potential reactor solutions. When coupled with a conformal wall, NT equilibria are confirmed to be less vertically stable than equivalent positive triangularity (PT) configurations. Unlike PT, their vertical stability is degraded at higher poloidal beta. Furthermore, improvements in vertical stability at low aspect ratio do not translate to the NT geometry. NT equilibria are stabilized in PT vacuum vessels due to the increased proximity of the plasma and the wall on the outboard side, but this scenario is found to be undesirable due to reduced vertical gaps which give less spatial margin for control recovery. Instead, we demonstrate that informed positioning of passively conducting plates can lead to improved vertical stability in NT configurations on par with stability metrics expected in PT scenarios. An optimal setup for passive plates in highly elongated NT devices is presented, where plates on the outboard side of the device reduce vertical instability growth rates to 16% of their baseline value. For lower target elongations, integration of passive stabilizers with divertor concepts can lead to significant improvements in vertical stability. Plates on the inboard side of the device are also uniquely enabled in NT geometries, providing opportunity for spatial separation of vertical stability coils and passive stabilizers.

70 PLASMA PHYSICS AND FUSION TECHNOLOGY↗
DOE OSTI2022

Finite magnetic well effects on resistive and drift-resistive ballooning modes in a shaped tokamak

The impact of plasma shaping through magnetic well modifications on the stability of resistive ballooning modes (RBMs) in tokamaks is analysed, also including finite diamagnetic flows. Furthermore, various limiting cases of the dispersion relation, obtained by matching the ballooning equation across the ideal and resistive layers, are analysed. It is found that stability is generally improved by the combination of vertical elongation and positive triangularity, although, in some cases, the growth rate of the unstable mode can be enhanced by these effects. Usually, vertically elongated plasmas with no triangularity are prone to exhibit worse stability properties. A value for the critical β above which RBMs are driven unstable is identified, and a connection with type-III ELM activity is established.

70 PLASMA PHYSICS AND FUSION TECHNOLOGY↗
DOE OSTI2020

HEXIM1 controls P-TEFb processing and regulates drug sensitivity in triple-negative breast cancer

The positive transcription elongation factor b (P-TEFb), composed of CDK9 and cyclin T, stimulates transcriptional elongation by RNA polymerase (Pol) II and regulates cell growth and differentiation. Recently, we demonstrated that P-TEFb also controls the expression of EMT regulators to promote breast cancer progression. In the nucleus, more than half of P-TEFb are sequestered in the inactive-state 7SK snRNP complex. Here, we show that the assembly of the 7SK snRNP is preceded by an intermediate complex between HEXIM1 and P-TEFb that allows transfer of the kinase active P-TEFb from Hsp90 to 7SK snRNP for its suppression. Down-regulation of HEXIM1 locks P-TEFb in the Hsp90 complex, keeping it in the active state to enhance breast cancer progression, but also rendering the cells highly sensitive to Hsp90 inhibition. Because HEXIM1 is often down-regulated in human triple-negative breast cancer (TNBC), these cells are particularly sensitive to Hsp90 inhibition. Our study provides a mechanistic explanation for the increased sensitivity of TNBC to Hsp90 inhibition.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2022

The Pristine dwarf galaxy survey – IV. Probing the outskirts of the dwarf galaxy Boötes I

ABSTRACT We present a new spectroscopic study of the dwarf galaxy Boötes I (Boo I) with data from the Anglo-Australian Telescope and its AAOmega spectrograph together with the Two Degree Field multi-object system. We observed 36 high-probability Boo I stars selected using Gaia Early Data Release 3 proper motions and photometric metallicities from the Pristine survey. Out of those, 27 are found to be Boo I stars, resulting in an excellent success rate of 75 per cent at finding new members. Our analysis uses a new pipeline developed to estimate radial velocities and equivalent widths of the calcium triplet lines from Gaussian and Voigt line profile fits. The metallicities of 16 members are derived, including 3 extremely metal-poor stars ([Fe/H] < −3.0), which translates into a success rate of 25 per cent at finding them with the combination of Pristine and Gaia. Using the large spatial extent of our new members that spans up to 4.1 half-light radii and spectroscopy from the literature, we find a systemic velocity gradient of 0.40 ± 0.10 km s−1 arcmin−1 and a small but resolved metallicity gradient of −0.008 ± 0.003 dex arcmin−1. Finally, we show that Boo I is more elongated than previously thought with an ellipticity of ϵ = 0.68 ± 0.15. Its velocity and metallicity gradients as well as its elongation suggest that Boo I may have been affected by tides, a result supported by direct dynamical modelling.

Longeard, Nicolas↗
DOE OSTI2022

Insights into the molecular mechanism of translation inhibition by the ribosome-targeting antibiotic thermorubin

Thermorubin (THR) is an aromatic anthracenopyranone antibiotic active against both Gram-positive and Gram-negative bacteria. It is known to bind to the 70S ribosome at the intersubunit bridge B2a and was thought to inhibit factor-dependent initiation of translation and obstruct the accommodation of tRNAs into the A site. Here, we show that thermorubin causes ribosomes to stall in vivo and in vitro at internal and termination codons, thereby allowing the ribosome to initiate protein synthesis and translate at least a few codons before stalling. Our biochemical data show that THR affects multiple steps of translation elongation with a significant impact on the binding stability of the tRNA in the A site, explaining premature cessation of translation. Our high-resolution crystal and cryo-EM structures of the 70S-THR complex show that THR can co-exist with P- and A-site tRNAs, explaining how ribosomes can elongate in the presence of the drug. Remarkable is the ability of THR to arrest ribosomes at the stop codons. Our data suggest that by causing structural re-arrangements in the decoding center, THR interferes with the accommodation of tRNAs or release factors into the ribosomal A site.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2026

Sparsomycin inhibits translation through a conserved mechanism across all domains of life

Abstract Sparsomycin (SPA) is a broad-spectrum inhibitor of protein synthesis with activity across all three domains of life. Although SPA has long been known to target the ribosomal peptidyl transferase center (PTC), previous structural studies suggested that SPA binds differently to bacterial ribosomes compared to their archaeal and eukaryotic counterparts—an unexpected conclusion given the high evolutionary conservation of the ribosomal catalytic center. Here, we show that SPA inhibits a majority of elongation-competent bacterial ribosomal complexes and present X-ray crystal structures of Thermus thermophilus 70S ribosomes stalled by SPA at the initiation and early elongation stages of translation. These structures reveal that SPA binds to the bacterial ribosome in a manner essentially identical to that observed in archaeal and eukaryotic ribosomes, establishing a unified structural mechanism of SPA action across all domains of life. In this conserved binding mode, SPA occupies the A-site cleft of the PTC and forms an extensive network of interactions with universally conserved ribosomal RNA nucleotides and the CCA-end of the P-site transfer RNA (tRNA), thereby stabilizing the peptidyl-tRNA substrate while sterically blocking accommodation of an incoming aminoacyl-tRNA. By clarifying the mode of action of SPA on the bacterial ribosome, our work provides a structural framework for the rational design of SPA derivatives with improved potency and bacterial specificity.

Paranjpe, Madhura N [Department of Biological Scie↗
DOE OSTI2020

Paraburkholderia solitsugae sp. nov. and Paraburkholderia elongata sp. nov., phenolic acid-degrading bacteria isolated from forest soil and emended description of Paraburkholderia madseniana

Two bacterial strains, 1N T and 5N T , were isolated from hemlock forest soil using a soluble organic matter enrichment. Cells of 1N T (0.65×1.85 µm) and 5N T (0.6×1.85 µm) are Gram-stain-negative, aerobic, motile, non-sporulating and exist as single rods, diplobacilli or in chains of varying length. During growth in dilute media (≤0.1× tryptic soy broth; TSB), cells are primarily motile with flagella. At higher concentrations (≥0.3× TSB), cells of both strains increasingly form non-motile chains, and cells of 5N T elongate (0.57×~7 µm) and form especially long filaments. Optimum growth of 1N T and 5N T occurred at 25–30 °C, pH 6.5–7.0 and <0.5% salinity. Results of comparative chemotaxonomic, genomic and phylogenetic analyses revealed that 1N T and 5N T were distinct from one another and their closest related type strains: Paraburkholderia madseniana RP11 T , Paraburkholderia aspalathi LMG 27731 T and Paraburkholderia caffeinilytica CF1 T . The genomes of 1N T and 5N T had an average nucleotide identity (91.6 and 91.3%) and in silico DNA–DNA hybridization values (45.8%±2.6 and 45.5%±2.5) and differed in functional gene content from their closest related type strains. The composition of fatty acids and patterns of substrate use, including the catabolism of phenolic acids, also differentiated strains 1N T and 5N T from each other and their closest relatives. The only ubiquinone present in strains 1N T and 5N T was Q-8. The major cellular fatty acids were C 16 : 0 , 3OH-C 16 : 0 , C 17 : 0 cyclo, C 19 : 0 cyclo ω8 c and summed features 2 (3OH-C 14 : 0 / C 16 : 1 iso I), 3 (C 16 : 1 ω6 c /ω7 c ) and 8 (C 18 : 1 ω7 c /ω6 c ). A third bacterium, strain RL16-012-BIC-B, was isolated from soil associated with shallow roots and was determined to be a strain of P. madseniana (ANI, 98.8%; 16S rRNA gene similarity, 100%). Characterizations of strain RL16-012-BIC-B (DSM 110723=LMG 31706) led to proposed emendments to the species description of P. madseniana . Our polyphasic approach demonstrated that strains 1N T and 5N T represent novel species from the genus Paraburkholderia for which the names Paraburkholderia solitsugae sp. nov. (type strain 1N T =DSM 110721 T =LMG 31704 T ) and Paraburkholderia elongata sp. nov. (type strain 5N T =DSM 110722 T =LMG 31705 T ) are proposed.

Microbiology↗
DOE OSTI2025

Synthesis of cobalt grown from Co-S eutectic in high magnetic fields

Samples of Co were grown directly in the ferromagnetic state under equilibrium conditions using a cobalt sulfide flux. Magnetic fields up to 9 T were applied during growth, and isolated Co products exhibit progressively elongated morphologies, from cubes to rectangular rods to needle-like tendrils with poorly-defined facets. The degree of elongation of the major axis was found to correlate with magnetic field direction, strength, and gradient. Two-dimensional X-ray diffraction data indicate some level of polycrystalline-like samples, and quantitative analyses (Le Bail and Rietveld) of the one-dimensional data confirm the presence of hcp and fcc phases. The magnetic responses indicate a partial alignment of the magnetic easy-axis of the hcp phase along the magnetic field present during growth.

36 MATERIALS SCIENCE↗
DOE OSTI2020

Influence of the vertex region on spin dynamics in artificial Kagome spin ice.

We present experimental and theoretical studies of spin-wave mode dynamics in artificial kagome spin ice vertices made of three identical 15-nm thick elongated Ni80Fe20 nanoislands (macrospins). We consider several possible configurations, from completely disjointed macrospins (full dipolar interelement interac-tions) to fully jointed macrospins (full dipolar-exchange interactions). Using angular-resolved magnetic field dependent broadband ferromagnetic resonance (FMR), we demonstrate the occurrence of a mode localized in the vertex region as indicated by the distinct behavior of the FMR spectra at different angles and configurations. Theoretical calculations using micromagnetic simulations support the existence, ori-gin, and behavior of this mode by interpreting it as a localized, quasi-uniform Kittel mode. Our findings pave the way for designing the most appropriate network consisting of ferromagnetic nanomagnets for specific application purposes in magnonics.

Bang, Wonbae↗
DOE OSTI2021

Antiferromagnetism and crystalline electric field excitations in tetragonal NaCeO 2

In this work, we investigate the crystal structure, magnetic properties, and crystalline electric field of tetragonal, I 4 1 / a m d , Na Ce O 2 . In this compound, Ce 3 + ions form a tetragonally elongated diamond lattice coupled by antiferromagnetic interactions ( Θ CW = - 7.69 K) that magnetically order below T N = 3.18 K. The Ce 3 + J = 5 / 2 crystalline electric field-split multiplet is studied via inelastic neutron scattering to parametrize a J eff = 1 / 2 ground state doublet composed of states possessing mixed | m z ) character. Neutron powder diffraction data reveal the onset of A -type antiferromagnetism with μ = 0.57 ( 2 ) μ B moments aligned along the c axis. The magnetic structure is consistent with the expectations of a frustrated Heisenberg J 1 - J 2 model on the elongated diamond lattice with effective exchange values J 1 > 4 J 2 and J 1 > 0 .

75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND↗
DOE OSTI2020

Cellulose nanofibrils and nanocrystals in confined flow: Single-particle dynamics to collective alignment revealed through scanning small-angle x-ray scattering and numerical simulations

Nanostructured materials made through flow-assisted assembly of proteinaceous or polymeric nanosized fibrillar building blocks are promising contenders for a family of high-performance biocompatible materials in a wide variety of applications. Optimization of these processes relies on improving our knowledge of the physical mechanisms from nano- to macroscale and especially understanding the alignment of elongated nanoparticles in flows. Here, we study the full projected orientation distributions of cellulose nanocrystals (CNCs) and nanofibrils (CNFs) in confined flow using scanning microbeam SAXS. For CNCs, we further compare with a simulated system of dilute Brownian ellipsoids, which agrees well at dilute concentrations. However, increasing CNC concentration to a semidilute regime results in locally arranged domains called tactoids, which aid in aligning the CNC at low shear rates, but limit alignment at higher rates. Similarly, shear alignment of CNF at semidilute conditions is also limited owing to probable bundle or flock formation of the highly entangled nanofibrils. Finally, this work provides a quantitative comparison of full projected orientation distributions of elongated nanoparticles in confined flow and provides an important stepping stone towards predicting and controlling processes to create nanostructured materials on an industrial scale.

36 MATERIALS SCIENCE↗
DOE OSTI2024

Towards Polymer-Free, Femto-Second Laser-Welded Glass/Glass Solar Modules

This project explores the use of femto-second (fs) lasers to form glass-to-glass welds for hermetically sealed, polymer-free solar modules. Low iron solar glass coupons were welded together without the use of glass filler using a fs laser with dedicated optics to elongate the focal plane parallel to the incident beam. The resulting welds were then stress tested to failure to reveal the critical stress intensity factor, KIc. These values were used in a structural mechanics model of a 1 m by 2 m glass/glass module under a simulated static load test. The results show that the fs laser welds are strong enough for a suitably framed module to pass the IEC 61215 static load test with a load of 5400 Pa. Key to this finding is that the module must be framed and braced, and the glass must be ribbed to allow pockets for the cells and welds inside the border of the module. The result is a module design that is completely polymer-free, hermetically sealed, has improved thermal properties, and is easily recycled.

femto-second laser welded↗
DOE OSTI2020

Heterotrimeric G‐protein, Gi1, is involved in the regulation of proliferation, neuronal migration, and dendrite morphology during cortical development in vivo

Abstract Heterotrimeric G‐proteins are composed of α, β, and γ subunits, and function as signal transducers. Critical roles of the α‐subunits of Gi/o family heterotrimeric G‐proteins, Gαi2, and Gαo1, have so far been reported in brain development and neurodevelopmental disorders. In this study, we tried to clarify the role of Gαi1, α‐subunit of another Gi/o family member Gi1, during corticogenesis, based on the recent identification of its gene abnormalities in neurodevelopmental disorders. In western blot analyses, Gαi1 was found to be expressed in mouse brain in a developmental stage‐dependent manner. Morphological analyses revealed that Gαi1 was broadly distributed in cerebral cortex with relatively high expression in the ventricular zone (VZ) at embryonic day (E) 14. Meanwhile, Gαi1 was enriched in membrane area of yet unidentified early mitotic cells in the VZ and the marginal zone at E14. Acute knockdown of Gαi1 with in utero electroporation in cerebral cortex caused cell cycle elongation of the neural progenitor cells and promoted their cell cycle exit. Gαi1‐deficient cortical neurons also exhibited delayed radial migration during corticogenesis, with abnormally elongated leading processes and hampered nucleokinesis. In addition, silencing of Gαi1 prevented basal dendrite development. The migration and dendritic phenotypes were at least partially rescued by an RNAi‐resistant version of Gαi1. Collectively, these results strongly suggest a crucial role of Gi1 in cortical development, and disturbance of its function may cause deficits in synaptic network formation, leading to neurodevelopmental disorders. image

Hamada, Nanako↗