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At least 199 records · Page 11

Optical interference for the guidance of cryogenic focused ion beam milling beyond the axial diffraction limit

Cryogenic focused ion beam (Cryo-FIB) milling has become a standard step in the cryogenic electron tomography (Cryo-ET) workflow and is required to thin cells to electron-semitransparency. However, this destructive process removes the vast majority of the cellular material and raises a critical question: what thin section should be preserved for Cryo-ET analysis? Using a tri-coincident cryogenic FIB-SEM-LM system, we identify an interferometric optical response that can be used for targeting lamella production to fluorescently labeled structures with accuracy beyond the diffraction limit. Here we demonstrate this approach using synthetic samples of fluorescent beads embedded in micron-scale droplets of amorphous ice. We then apply the approach to capture virions inside host cells. Successful targeting is confirmed by Cryo-ET revealing clusters of virions in intracellular vesicles. The method does not require any fluorescent fiducials or axial registration and can be performed on any fluorescently labeled structure that is visible in widefield fluorescence microscopy.

Cryoelectron microscopy↗

Dual ion beam deposition of carbon films with diamondlike properties

A single and dual ion beam system was used to generate amorphous carbon films with diamond like properties. A methane/argon mixture at a molar ratio of 0.28 was ionized in the low pressure discharge chamber of a 30-cm-diameter ion source. A second ion source, 8 cm in diameter was used to direct a beam of 600 eV Argon ions on the substrates (fused silica or silicon) while the deposition from the 30-cm ion source was taking place. Nuclear reaction and combustion analysis indicate H/C ratios for the films to be 1.00. This high value of H/C, it is felt, allowed the films to have good transmittance. The films were impervious to reagents which dissolve graphitic and polymeric carbon structures. Although the measured density of the films was approximately 1.8 gm/cu cm, a value lower than diamond, the films exhibited other properties that were relatively close to diamond. These films were compared with diamondlike films generated by sputtering a graphite target.

Mirtich, M. J.↗

Fabrication of OSOS cells by neutral ion beam sputtering

Oxide semiconductor on silicon (OSOS) solar cells have been fabricated from various indium tin oxide (In2O3)x(SnO2)1-x compositions sputtered onto p-type single crystal silicon substrates with a neutralized argon ion beam. High temperature processing or annealing was not required. The highest efficiency was achieved with x = 0.91 and was 12 percent. The cells are environmentally rugged, chemically stable, and show promise for still higher efficiencies. Moreover, the ion beam sputtering fabrication technique is amenable to low cost, continuous processing.

Burk, D. E.↗

Nonlinear transient neutralization theory of ion beams with dissipation

An analytical theory of nonlinear neutralization waves generated by injection of electrons from a grid in the direction of a homogeneous ion beam of uniform velocity and infinite extension is presented. The electrons are assumed to interact with the ions through the self-consistent space charge field and by strong collective interactions. The associated nonlinear boundary-value problem is solved in closed form by means of a von Mises transformation. It is shown that the electron gas moves into the ion space in the form of a discontinuous neutralization wave. This periodic wave structure is damped out by intercomponent momentum transfer, i.e., after a few relaxation lengths a quasi-neutral beam results. The relaxation scale in space agrees with neutralization experiments of rarefied ion beams, if the collective momentum transfer between the electron and ion streams is assumed to be of the Buneman type.

Wilhelm, H. E.↗

Electromagnetic ion beam instabilities

The linear theory of electromagnetic instabilities driven by an energetic ion beam streaming parallel to a magnetic field in a homogeneous Vlasov plasma is considered. Numerical solutions of the full dispersion equation are presented. At propagation parallel to the magnetic field, there are four distinct instabilities. A sufficiently energetic beam gives rise to two unstable modes with right-hand polarization, one resonant with the beam, the other nonresonant. A beam with sufficiently large T (perpendicular to B)/T (parallel to B) gives rise to the left-hand ion cyclotron anisotropy instability at relatively small beam velocities, and a sufficiently hot beam drives unstable a left-hand beam resonant mode. The parametric dependences of the growth rates for the three high beam velocity instabilities are presented here. In addition, some properties at oblique propagation are examined. It is demonstrated that, as the beam drift velocity is increased, relative maxima in growth rates can arise at harmonics of the ion cyclotron resonance for both right and left elliptically polarized modes.

Gary, S. P.↗

High-Vacuum Condenser Design: Experimental Effects from Cesium and Mercury Ion Beams

Effects of variable-energy electrostatic rocket-engine ion exhaust beams that pertain to the design of high-vacuum condensers are discussed. Three ion-engine beam sources, using cesium and mercury as propellants, were operated at energy levels of 200 to 9000 electron volts and currents of 0.002 to 0.200 ampere. Five condenser geometries, having surface areas from 0.287 to 5.76 square meters, were tested. Values for a function of the accommodation and condensation coefficients, which appears in an existing theoretical analysis, were determined. Although exhibiting rather wide variations in some tests, the values were estimated at approximately 0.15 for cesium and 0.015 for mercury. A method of estimating condenser surface area requirements is given.

MERCURY /METAL/↗

Rate-induced aging effects on Parallel-Plate Avalanche Counter (PPAC) caused by heavy ion beams

The Facility for Rare Isotope Beams (FRIB) is one of the premier scientific user facilities for nuclear science with radioactive beams, capable of producing most (approximately 80%) of the isotopes expected to exist, from oxygen to uranium, at energies up to 200 MeV/u. With the increase in beam power from the present 10 kW to the planned 400 kW, FRIB experiments are about to enter a new era. An unprecedented rate capability as well as stable performance of all the planned instrumentation intended for beam diagnostics and beam tuning is required at the expected high beam intensities (> 1 MHz). A summary of aging phenomena at high heavy-ion beam rates observed in the Advanced Rare Isotope Separator (ARIS) detectors for beam diagnostics, including Parallel Plate Avalanche Counters (PPAC) and plastic scintillation for time-of-flight measurements, is discussed. Current research and development project to mitigate rate-induced aging are presented.

Aging effects↗

Adhesive bonding of ion beam textured metals and fluoropolymers

An electron bombardment argon ion source was used to ion etch various metals and fluoropolymers. The metal and fluoropolymers were exposed to (0.5 to 1.0) keV Ar ions at ion current densities of (0.2 to 1.5) mA/sq cm for various exposure times. The resulting surface texture is in the form of needles or spires whose vertical dimensions may range from tenths to hundreds of micrometers, depending on the selection of beam energy, ion current density, and etch time. The bonding of textured surfaces is accomplished by ion beam texturing mating pieces of either metals or fluoropolymers and applying a bonding agent which wets in and around the microscopic cone-like structures. After bonding, both tensile and shear strength measurements were made on the samples. Also tested, for comparison's sake, were untextured and chemically etched fluoropolymers. The results of these measurements are presented.

Mirtich, M. J.↗

Simulation study of ion beam used to produce Mo-99

An 820 mA CW positive ion source is being developed to produce Mo-99 using the fusion of deuterium and tritium ion beams on a rotating target to produce neutrons for use in the production of radiopharmaceuticals. The ion source consists of an RF plasma source, a multi-aperture extractor, and 300 kV accelerating column. This paper will describe a simulation study of the beam through the extractor grid and the accelerator to the target. The uniformity of beam distribution on the target is an important aspect of the simulation.

43 PARTICLE ACCELERATORS↗

The Relationship of Ion Beams and Fast Flows in the Plasma Sheet Boundary Layer

We report new findings on the behavior of plasmas in the vicinity of the plasma sheet boundary layer (PSBL). A large geometrical factor detector on WIND (3D plasma experiment) has discovered a unidirectional ion beam streaming in the tailward direction missed by previous observations. This tailward beam is as intense as the earthward streaming beam and it is found just inside the outer edge of the PSBL where earthward streaming beams are observed. The region where this tailward beam is observed includes an isotropic plasma component which is absent in the outer edge where earthward streaming beams are found. When these different distributions are convolved to calculate the velocity moments, fast flows (greater than 400 km/s) result in the earthward direction and much slower flows (less than 200 km/s) in the tailward direction. These new findings are substantially different from previous observations. Thus, the interpretation of fast flows and earthward and counterstreaming ion beams in terms of a neutral line model must be reexamined.

Parks, G. K.↗

Reaction of O2(+)(X 2Pi sub g) with H2, D2, and HD - Guided ion beam studies, MO correlations, and statistical theory calculations

Guided ion-beam mass spectrometry is used to examined the reactions of vibrationally cold ground-state O2(+)(X 2Pi sub g) with H2, D2, and HD. The energy dependence of the absolute integral cross sections from thermal energy to over 4 eV are measured in the center-of-mass frame of reference. Results are also presented for internally excited O2(+) ions reacting with D2 and HD. The results are consistent with the dominant state being the a 4Pi sub u electronic state. The experimental excitation functions are analyzed in detail and interpreted by extending the molecular orbital correlation arguments of Mahan (1971) and by comparison with results of statistical phase space theory and with a theory that predicts a tight transition state.

Weber, M. E.↗

High power ion beam generator systems and methods

Provided herein are high energy ion beam generator systems and methods that provide low cost, high performance, robust, consistent, uniform, low gas consumption and high current/high-moderate voltage generation of neutrons and protons. Such systems and methods find use for the commercial-scale generation of neutrons and protons for a wide variety of research, medical, security, and industrial processes.

Kobernik, Arne↗

High power ion beam generator systems and methods

Provided herein are high energy ion beam generator systems and methods that provide low cost, high performance, robust, consistent, uniform, low gas consumption and high current/high-moderate voltage generation of neutrons and protons. Such systems and methods find use for the commercial-scale generation of neutrons and protons for a wide variety of research, medical, security, and industrial processes.

Kobernik, Arne↗

Dual-ion-beam deposition of carbon films with diamond-like properties

A single and dual ion beam system was used to generate amorphous carbon films with diamond like properties. A methane/argon mixture at a molar ratio of 0.28 was ionized in the low pressure discharge chamber of a 30-cm-diameter ion source. A second ion source, 8 cm in diameter was used to direct a beam of 600 eV Argon ions on the substrates (fused silica or silicon) while the deposition from the 30-cm ion source was taking place. Nuclear reaction and combustion analysis indicate H/C ratios for the films to be 1.00. This high value of H/C, it is felt, allowed the films to have good transmittance. The films were impervious to reagents which dissolve graphitic and polymeric carbon structures. Although the measured density of the films was approximately 1.8 gm/cu cm, a value lower than diamond, the films exhibited other properties that were relatively close to diamond. These films were compared with diamond like films generated by sputtering a graphite target.

Mirtich, M. J.↗

Nonlinear evolution of the ion-ion beam instability

The criterion for the existence of vortex-like ion phase-space configurations, as obtained by a standard pseudopotential method, is found to coincide with the criterion for the linear instability for two (cold) counterstreaming ion beams. A nonlinear equation is derived, which demonstrates that this instability actually evolves into such phase-space configurations. A small, but nonzero, ion temperature turns out to be essential for the saturation into stationary structures

Pecseli, H. L.↗

Electromagnetic ion beam instabilities - Growth at cyclotron harmonic wave numbers

The linear theory of electromagnetic ion beam instabilities for arbitrary angles of propagation is studied, with an emphasis on the conditions necessary to generate unstable modes at low harmonics of the ion cyclotron resonance condition. The present results extend the analysis of Smith et al. (1985). That paper considered only the plasma parameters at a time during which harmonic wave modes were observed in the earth's foreshock. The parameters of that paper are used as the basis of parametric variations here to establish the range of beam properties which may give rise to observable harmonic spectra. It is shown that the growth rates of both left-hand and right-hand cyclotron harmonic instabilities are enhanced by an increase in the beam temperature anisotropy and/or the beam speed. Decreases in the beam density and/or the core-ion beta reduce the overall growth of the cyclotron harmonic instabilities but favor the growth of these modes over the growth of the nonresonant instability and thereby enhance the observability of the harmonics.

Smith, Charles W.↗