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The nematode Caenorhabditis elegans was exposed to natural space radiation using the ESA Biorack facility aboard Spacelab on International Microgravity Laboratory 1, STS-42. For the major experimental objective dormant animals were suspended in buffer or on agar or immobilized next to CR-39 plactic nuclear track detectors to correlate fluence of HZE particles with genetic events.
In this summary, we present the main conclusions and results of a design study conducted by a group of 25 students at the University of Maryland. The students, all participants in the spring 1988 courses ENEE488Q and ENEE418 in the Electrical Engineering Department, considered the problem of designing a free-flying space robot for applications in satellite servicing. In addition to the paper study, a subgroup of eight students undertook the ambitious task of designing and building a laboratory testbed for the concept of a free flyer. This subgroup has already completed the main fabrication of the mechanical hardware and most of the onboard electronics. When completed by the end of the summer, the testbed will consist of a seven-degree-of-freedom dual-armed planar robot that will float on an air table and carry out various commanded tasks. All the fabrication and testing is being conducted in the Intelligent Servosystems Laboratory at the University of Maryland. The project team split up into subgroups as follows: (1) articulation concepts 1 and 2, to study competing ideas for the overall systems architecture and manipulator design; (2) real time control systems group; (3) a computer graphics group to provide animations of a planar design; (4) a mechanical hardware design group for the laboratory testbed; and (5) a control systems hardware design group for the same.
Introduction: TheraSphere ® microspheres containing yttrium 90 Y are among many radioembolization agents used clinically to reduce liver tumor burden, and their effects on cancer volume reduction are well-established. At the same time, concerns about off target tissue injury often limit their use. Deeper investigation into tissue distribution and long-term impact of these microspheres could inform us about additional ways to use them in practice. Methods: Healthy rat liver and rabbit liver tumor samples from animals treated with TheraSpheres were sectioned and their elemental maps were generated by X-ray fluorescence microscopy (XFM) at the Advanced Photon Source (APS) synchrotron at Argonne National Laboratory (ANL). Results: Elemental imaging allowed us to identify the presence and distribution of TheraSpheres in animal tissues without the need for additional sample manipulation or staining. Ionizing radiation produced by 90 Y radioactive contaminants present in these microspheres makes processing TheraSphere treated samples complex. Accumulation of microspheres in macrophages was observed. Conclusions: This is the first study that used XFM to evaluate the location of microspheres and radionuclides in animal liver and tumor samples introduced through radioembolization. XFM has shown promise in expanding our understanding of radioembolization and could be used for investigation of human patient samples in the future.
The majority of undergraduate and graduate students in nuclear science and engineering take a general course in nuclear instrumentation where one of the topics is typically in gamma ray spectrometry. Rarely are courses offered solely on this subject matter other than intensified workshops conducted by the International Atomic Energy Agency (IAEA) or private companies which can be expensive especially for large groups of students. Currently there are many world-wide projects in routine monitoring and advanced research that employ gamma rays. Some but not an exhaustive list of these topics include neutron activation analysis, naturally occurring radioactivity materials (NORM), nuclear forensics, nuclear security, in-situ gamma ray detection, galactic gamma ray detection, health physics and advanced instrumentation. At the Nuclear Engineering Teaching Laboratory at the University of Texas at Austin, many of our students are involved in an array of research topics that involve gamma ray spectrometry in their Masters of Science theses, PhD dissertations and special topics courses. It was decided to invest the time and effort to develop a course with a strong technical component to complement their research activities. A detailed stand-alone course in gamma ray spectrometry that includes a series lectures in modular form has been developed. As well we have incorporated fourteen advanced animations to better explain some of the fundamental physical processes and experimental set-ups. Six laboratory sessions and multiple homework assignments have also been established. This course is applicable to senior undergraduate students, graduate students, and novice researchers the above mentioned fields in a traditional class/laboratory setting or for intense one to two week workshops. A great deal of effort was undertaken to prepare a stand-alone course in gamma ray spectrometry which includes many of the fundamental aspects as well as the more advanced instrumentation. Animations of gamma ray interactions and experimental set ups were also developed. Six laboratories and six assignments were also conceived. Every topic has its own module thus a 'pick and choose' subset of lectures can be given to different levels of understanding. This course is applicable to senior undergraduate students, graduate students, novice researchers in neutron activation analysis and naturally occurring radioactive material, and for intense one to two week workshops. (authors)
Low dose ionizing radiation effects are difficult to study in human population because of the numerous confounding factors such as genetic and lifestyle differences. Research in mammalian model systems and in vitro is generally used in order to overcome this difficulty. In this program project three projects have joined together to investigate effects of low doses of ionizing radiation. These are doses at and below 10 cGy of low linear energy transfer ionizing radiation such as X-ray and gamma rays. This project was focused on cellular signaling associated with nuclear factor kappa B (NFkB) and mitochondria - subcellular organelles critical for cell aging and aging-like changes induced by ionizing radiation. In addition to cells in culture this project utilized animal tissues accumulated in a radiation biology tissue archive housed at Northwestern University (http://janus.northwestern.edu/janus2/index.php). Major trust of Project 1 was to gather all of the DoE sponsored irradiated animal (mouse, rat and dog) data and tissues under one roof and investigate mitochondrial DNA changes and micro RNA changes in these samples. Through comparison of different samples we were trying to delineate mitochondrial DNA quantity alterations and micro RNA expression differences associated with different doses and dose rates of radiation. Historic animal irradiation experiments sponsored by DoE were done in several national laboratories and universities between 1950’s and 1990’s; while these experiments were closed data and tissues were released to Project 1. Project 2 used cells in culture to investigate effects that low doses or radiation have on NFκB and its target genes manganese superoxide dismutase (MnSOD) and genes involved in cell cycle: Cyclins (B1 and D1) and cyclin dependent kinases (CDKs). Project 3 used cells in culture such as “normal” human cells (breast epithelial cell line MCF10A cells and skin keratinocyte cells HK18) and mouse embryo fibroblast (mef) cells to focus on role of NFkB protein and several other proteins such as survivin (BIRC5) in radiation dependent regulation of tumor necrosis factor alpha (TNFα) and its downstream signaling.
Novel Farms, Inc., as a participant of the Cyclotron Road Lab-Embedded Entrepreneurship Program, investigated the performance of various animal cell lines and animal cell media formulations using its proprietary scaffolds as a growth substratum towards the development of economically-feasible cultivated meat production. As an alternative to conventionally farmed animal meat, cultivated meat production has the potential to yield a marginal carbon footprint while providing sustainable and cruelty-free animal protein. The prohibitively high cost of the growth factors necessary in animal cell culture media constitutes the primary barrier that hinders the economically viable production of cultivated meat products. Furthermore, scaffolding is required to create structured meat products, but current options are unsuitable for cost-effective large-scale production of cultivated meat. Novel Farms has developed a proprietary technology to produce scaffolding that has the potential to reduce production costs while providing an ideal 3D surface for cell growth. Developing cost-efficient methods for cultivated meat production will be vital in satisfying the growing demand for animal protein while avoiding the deforestation, carbon emissions, biodiversity loss and other negative outcomes of industrial animal farming.
There is a critical need for an inactivation method that completely inactivates pathogens at the time of sample collection while maintaining the nucleic acid quality required for diagnostic PCR testing. This inactivation method is required to alleviate concerns about transmission potential, minimize shipping complications and cost, and enable testing in lower containment laboratories, thereby enhancing disease diagnostics through improved turn-around time. This study evaluated a panel of 10 surrogate viruses that represent highly pathogenic animal diseases. These results showed that a commercial PrimeStore® molecular transport media (PSMTM) completely inactivated all viruses tested by >99.99%, as determined by infectivity and serial passage assays. However, the detection of viral nucleic acid by qRT-PCR was comparable in PSMTM and control-treated conditions. These results were consistent when viruses were evaluated in the presence of biological material such as sera and cloacal swabs to mimic diagnostic sample conditions for non-avian and avian viruses, respectively. The results of this study may be utilized by diagnostic testing laboratories for highly pathogenic agents affecting animal and human populations. These results may be used to revise guidance for select agent diagnostic testing and the shipment of infectious substances.
The design of a specialized analog computer for on-line determination of cardiac stroke volume by means of a modified version of the pressure pulse contour method is presented. The design consists of an analog circuit for computation and a timing circuit for detecting necessary events on the pressure waveform. Readouts of arterial pressures, systolic duration, heart rate, percent change in stroke volume, and percent change in cardiac output are provided for monitoring cardiac patients. Laboratory results showed that computational accuracy was within 3 percent, while animal experiments verified the operational capability of the computer. Patient safety considerations are also discussed.
A prototype Lisp-based soft real-time object-oriented Graphical User Interface for control system development is presented. The Graphical User Interface executes alongside a test system in laboratory conditions to permit observation of the closed loop operation through animation, graphics, and text. Since it must perform interactive graphics while updating the screen in real time, techniques are discussed which allow quick, efficient data processing and animation. Examples from an implementation are included to demonstrate some typical functionalities which allow the user to follow the control system's operation.
In order to estimate radiation exposure in space, experiments were conducted during the 1st International Microgravity Laboratory (IML-1) mission in order to isolate genetic changes in animal cells caused by cosmic rays. The space measurements were evaluated against results from synthetic cosmic rays produced by particle accelerators on the ground. The biological material used was the tiny soil nematode, Caenorhabditis elegans. The measurements were made by thermoluminescent detectors and plastic nuclear track detectors. The development and the chromosome mechanics in microgravity were studied, and the mutagenesis induced by radiation exposure was analyzed. The results showed that there are no obvious differences in the development, behavior and chromosome mechanics, as a function of gravity unloading (reproduction, self-fertilization and mating of males with hermaphrodites, gross anatomy, symmetry and gametogenesis, pairing, disjoining and recombination of chromosomes). A variety of mutants were isolated, and it was noted that mutants isolated from regions of identified high particles were more severely affected than those isolated by random screening. Linear energy transfer particles seem to favor large scale genetic lesions.
Across two consecutive Global Wildlife Data Sharing Conferences supported by PAX sapiens—Year 1 (May 2024) at Pacific Northwest National Laboratory and Year 2 (2025) in Ciudad Real, Spain—the initiative converted wildlife data sharing from aspiration into operational reality, producing measurable impacts in platform development, data mobilization, standards harmonization, and international partnership formation. The conferences addressed a critical gap in global health security: while 75% of emerging infectious diseases affect both humans and animals and over 60% originate in wildlife, wildlife health surveillance has historically lagged behind human and agricultural sectors due to fragmented databases, inconsistent terminology, uneven capacity, and limited cross-border coordination. By convening practitioners, government agencies, international organizations, academic institutions, and NGOs, the GWDSC catalyzed trust-based relationships and practical workflows that enable earlier detection, better risk assessment, and more effective prevention of threats at the wildlife–domestic animal–human–environment interface.
Florida Atlantic University developed an underwater optical monitoring system prototype - Unobtrusive Multi-static Serial LiDAR Imager (UMSLI) - suitable for marine energy full project lifecycle observation (baseline, commissioning, and decommissioning), with an automated real-time classification of marine animals. With precursor 2014 DOE funding (award DE-EE0006787), a prototype UMSLI was demonstrated in a controlled laboratory environment and achieved a TRL 6. This EERE DE-EE0007828 award aimed to both increase the TRL of the UMSLI by improving the technology performance (e.g., increase distance of marine animal target detection capability, add additional species classification capabilities, improve the system performance during the day, etc.) and reduce the cost. The UMSLI presents a novel application of underwater distributed Light Detection And Ranging (LiDAR), an advanced remote sensing method that uses light in the form of laser pulses for an application, this paired with an algorithm provides 360 degrees underwater detection, imaging, and classification of marine life. This solution for underwater monitoring of biota preserves the advantages of traditional optical and acoustic solutions while overcoming many associated disadvantages for marine energy site environmental monitoring, such as difficulties in species detection and classification in low light or night and turbid environments. This new approach is a purposefully designed, reconfigurable adaptation of an existing class 3B laser technology into one UMSLI instrument that can be easily mounted on or around different classes of marine energy equipment, such as devices to capture ocean current energy or wave energy. The system uses relatively low average power, utilizes far-red (> 635nm) laser illumination to be invisible and eye-safe to marine animals, is compact, and cost-effective. The equipment is designed for long-term, maintenance-free operations (i.e., current design is targeting more than 7 days continuous operation), to inherently generate a sparse primary dataset that only includes detected anomalies, and to allow robust real-time automated animal classification and identification with a low data bandwidth requirement. The technology’s overarching goal for application, is a system that can be deployed to collect pre-installation baseline species observations at a proposed marine energy deployment site with minimal post-processing overhead. The envisioned system will also produce high-resolution imagery of marine animals through a wide range of conditions and support automated tracking and notification of the presence of managed animals within established perimeters of marine energy equipment to satisfy deployed marine energy projects’ endangered and threatened species monitoring requirements. Through the current project, we demonstrated the UMSLI prototype in an operational environment and increased the UMSLI’s Technology Readiness Level from 6 to 7. The project resulted in many novel technologies, including: 1) an eye-safe red laser based, low-cost LiDAR system that can detect targets up to 10 meters distance; 2) the GAN-based machine learning underwater LiDAR image enhancement technique (this is the first known application of GAN technique in underwater LiDAR); 3) LiDAR-based real-time automated detection capabilities; and 4) a template matching based automated classification tool. These technologies build a solid foundation for future efforts to develop an extended range electro-optical monitoring system suitable for marine energy deployments. In addition to technology development, a key lesson learned is that addressing regulatory and safety requirements must be front and center in any marine energy monitoring applications.
The present and proposed capabilities of the Central Image Processing Laboratory, which provides a powerful resource for the advancement of programs in missile technology, space science, oceanography, and biomedical image analysis, are discussed. The use of image digitizing, digital image processing, and digital image output permits a variety of functional capabilities, including: enhancement, pseudocolor, convolution, computer output microfilm, presentation graphics, animations, transforms, geometric corrections, and feature extractions. The hardware and software of the Image Processing Laboratory, consisting of digitizing and processing equipment, software packages, and display equipment, is described. Attention is given to applications for imaging systems, map geometric correction, raster movie display of Seasat ocean data, Seasat and Skylab scenes of Nantucket Island, Space Shuttle imaging radar, differential radiography, and a computerized tomographic scan of the brain.
This paper will share the creation, implementation, and modification of an online college level general biology laboratory course offered for non-science majors as a part of a General Education Curriculum. The ability of professors to develop quality online laboratories will address a growing need in Higher Education as more institutions combine course sections and look for suitable alternative course delivery formats due to declining departmental budgets requiring reductions in staffing, equipment, and supplies. Also, there is an equal or greater need for more professors to develop the ability to create online laboratory experiences because many of the currently available online laboratory course packages from publishers do not always adequately parallel on-campus laboratory courses, or are not as aligned with the companion lecture sections. From a variety of scientific simulation and animation web sites, professors can easily identify material that closely fit the specific needs of their courses, instructional environment, and students that they serve. All too often, on-campus laboratory courses in the sciences provide what are termed confirmation experiences that do NOT allow students to experience science as would be carried out by scientists. Creatively developed online laboratory experiences can often provide the type of authentic investigative experiences that are not possible on-campus due to the time constraints of a typical two-hour, once-per-week-meeting laboratory course. In addition, online laboratory courses can address issues related to the need for students to more easily complete missing laboratory assignments, and to have opportunities to extend introductory exercises into more advanced undertakings where a greater sense of scientific discovery can be experienced. Professors are strongly encourages to begin creating online laboratory exercises for their courses, and to consider issues regarding assessment, copyrights, and Intellectual Property concerns.
Biological studies hardware for Spacelab flights are described. The research animal holding facility has modular construction and is installed on a single ESA rack. A biotelemetry system will provide body temperature and EKG/heart rate data from a radio transmitter surgically implanted in the animals' stomachs. A plant growth unit (PGU) will be used to study micro-g plant lignin growth. The PGU is automated and can carry as many as 96 plants. A general purpose work station (GPWS) biohazard cabinet will be flown on Spacelab 4 to control liquid and chemical vapors released during experimentation. Spacelab 4 will be the premier flight of actual animal studies comprising measurements of hematology, muscle biochemistry, blood circulation, fluids and electrolytes, vestibular adaptation, etc., using rats and squirrel monkeys as subjects.
Here we describe a new in-laboratory analyzer based phase contrast-imaging (ABI) instrument using a conventional X-ray tube source (CXS) aimed at bio-medical imaging applications. Phase contrast-imaging allows visualization of soft tissue details usually obscured in conventional X-ray imaging. The ABI system design and major features are described in detail. The key advantage of the presented system, over the few existing CXS ABI systems, is that it does not require high precision components, i.e., CXS, X-ray detector, and electro-mechanical components. To overcome a main problem introduced by these components, identified as temperature stability, the system components are kept at a constant temperature inside of three enclosures, thus minimizing the electrical and mechanical thermal drifts. This is achieved by using thermoelectric (Peltier) cooling/heating modules that are easy to control precisely. For CXS we utilized a microfocus X-ray source with tungsten (W) anode material. In addition the proposed system eliminates tungsten's multiple spectral lines by selecting monochromator crystal size appropriately therefore eliminating need for the costly mismatched, two-crystal monochromator. The system imaging was fine-tuned for tungsten Kα{sub 1} line with the energy of 59.3 keV since it has been shown to be of great clinical significance by a number of researchers at synchrotron facilities. In this way a laboratory system that can be used for evaluating and quantifying tissue properties, initially explored at synchrotron facilities, would be of great interest to a larger research community. To demonstrate the imaging capability of our instrument we use a chicken thigh tissue sample.
The challenge of optimizing both performance and safety in nanomaterials hinges on our ability to resolve which structural features lead to desired properties. It has been difficult to draw meaningful conclusions about biological impacts from many studies of nanomaterials due to the lack of nanomaterial characterization, unknown purity, and/or alteration of the nanomaterials by the biological environment. To investigate the relative influence of core size, surface chemistry, and charge on nanomaterial toxicity, we tested the biological response of whole animals exposed to a matrix of nine structurally diverse, precision-engineered gold nanoparticles (AuNPs) of high purity and known composition. Members of the matrix include three core sizes and four unique surface coatings that include positively and negatively charged headgroups. Mortality, malformations, uptake, and elimination of AuNPs were all dependent on these parameters, showing the need for tightly controlled experimental design and nanomaterial characterization. Results presented herein illustrate the value of an integrated approach to identify design rules that minimize potential hazard.
The relationship between domestic cats and humans may facilitate transmission of viruses between the two hosts. Metagenomic analyses of 70 urban feline swab samples identified six high-quality draft genomes from felid alphaherpesvirus 1 and feline foamy virus.