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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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At least 199 records · Page 11
All sourcesNASA NTRSDOE OSTI
NASA NTRS1977

Nuclear emulsion measurements of the dose contribution from tissue disintegration stars on the Apollo-Soyuz mission

A total of 996 disintegration stars were prong-counted in two 100 micron llford K.2 emulsions from the dosimeter of the Docking Pilot on Apollo-Soyuz. The change of slope of the distribution at a prong number of about 6 or 7 indicates 219 stars as originating in gelatin. Applying the QF values set forth in official regulations to the energy spectra of the proton and a alpha prongs of the gelatin stars leads to a tissue star dose of 7.8 millirad or 45 millirem. The quoted values do not include the dose contribution from star-produced neutrons since neutrons do not leave visible prongs in emulsion. Nuclear theory, in good agreement with measurements of galactic radiation in the earth's atmosphere, indicates that the dose equivalent from neutrons is about equal to the one from all ionizing secondaries of stars. Application of this proposition to the star prong spectrum found on Apollo-Soyuz would set the total tissue star dose for the mission at approximately 90 millirem.

Schaefer, H. J.↗
NASA NTRS1977

Effect of surface texture by ion beam sputtering on implant biocompatibility and soft tissue attachment

The objectives in this report were to use the ion beam sputtering technique to produce surface textures on polymers, metals, and ceramics. The morphology of the texture was altered by varying both the width and depth of the square pits which were formed by ion beam erosion. The width of the ribs separating the pits were defined by the mask used to produce the texture. The area of the surface containing pits varies as the width was changed. The biological parameters used to evaluate the biological response to the texture were: (1) fibrous capsule and inflammatory response in subcutaneous soft tissue; (2) strength of the mechanical attachment of the textured surface by the soft tissue; and (3) morphology of the epidermal layer interfacing the textured surface of percutaneous connectors. Because the sputter yield on teflon ribs was approximately an order of magnitude larger than any other material the majority of the measurements presented in the report were obtained with teflon.

Gibbons, D. F.↗
NASA NTRS1978

The rate of synthesis and decomposition of tissue proteins in hypokinesia and increased muscular activity

During hypokinesia and physical loading (swimming) of rats, the radioactivity of skeletal muscle, liver, kidney, heart, and blood proteins was determined after administration of radioactive amino acids. Tissue protein synthesis decreased during hypokinesia, and decomposition increased. Both synthesis and decomposition increased during physical loading, but anabolic processes predominated in the total tissue balance. The weights of the animals decreased in hypokinesia and increased during increased muscle activity.

Fedorov, I. V.↗
NASA NTRS1978

A note on the tissue star dose in personnel radiation monitoring in space

Secondaries from nuclear interactions of high energy primaries in the body tissues themselves contribute substantially to the astronaut's radiation exposure in space. The so-called tissue star dose is assessed from the prong number distribution of disintegration stars in emulsion. Prong counts of 1,000 emulsion stars from the Apollo-Soyuz mission reported earlier were re-evaluated. The original scores were divided into sets of 250, 500, 750, and 1,000 emulsion stars and the respective prong number distributions established. The statistical error of the gelatin star number for the four consecutively larger was found to vary, on the 67 percent confidence level, from + or - 25 percent for the count of 250 emulsion stars to + or - 11 percent for 1,000 stars. Seen in the context of the other limitations of the experimental design, the lowest effort of prong-counting 250 stars appears entirely appropriate.

Schaefer, H. J.↗
NASA NTRS1978

Controlled cellular energy conversion in brown adipose tissue thermogenesis

Brown adipose tissue serves as a model system for nonshivering thermogenesis (NST) since a) it has as a primary physiological function the conversion of chemical energy to heat; and b) preliminary data from other tissues involved in NST (e.g., muscle) indicate that parallel mechanisms may be involved. Now that biochemical pathways have been proposed for brown fat thermogenesis, cellular models consistent with a thermodynamic representation can be formulated. Stated concisely, the thermogenic mechanism in a brown fat cell can be considered as an energy converter involving a sequence of cellular events controlled by signals over the autonomic nervous system. A thermodynamic description for NST is developed in terms of a nonisothermal system under steady-state conditions using network thermodynamics. Pathways simulated include mitochondrial ATP synthesis, a Na+/K+ membrane pump, and ionic diffusion through the adipocyte membrane.

Horowitz, J. M.↗
NASA NTRS1979

Tissue gas and blood analyses of human subjects breathing 80% argon and 20% oxygen

Eight human volunteers, individually studied in a hyperbaric chamber, breathed: (1) air at 1 ATA; (2) 80% argon and 20% oxygen at 1 ATA for 30 min; (3) air at 1 ATA for 30 min; (4) 100% O2 at 1 ATA for 30 min; (5) air at 1 ATA for 30 min; (6) 100% O2 at 2 ATA for 60 min; and (7) 80% argon and 20% oxygen at 1 ATA for 30 min. Oxygen, carbon dioxide, nitrogen, and argon tensions were measured in muscle and subcutaneous tissue by mass spectroscopic analyses. Venous blood obtained at regular intervals was analyzed for coagulation and fibrinolytic factors. Inert gas narcosis was not observed. After breathing argon for 30 min, muscle argon tensions were almost three times the subcutaneous tensions. Argon wash-in mirrored nitrogen wash-out. Argon wash-in and wash-out had no effect on tissue PO2 or PCO2. Coagulation and fibrinolytic changes usually associated with vascular bubbles were absent.

Horrigan, D. J.↗
NASA NTRS1979

Effect of prolonged hypokinesia on tissue blood flow

The influence of hypokinesia on the blood flow in the tissues of rabbits was studied. Motor activity of animals was restricted during 90 days and blood flow recorded through resorption rate of NaI-131. Perfusion of tissues under the influence of hypokinesia was found to be reduced.

Levites, Z. P.↗
NASA NTRS1980

Effectiveness of using thyrocalcitonin for the prevention of a calcium metabolic disorder in the mineralized tissues of rabbits with 30 days hypokinesia

A 30 day hypokinesia in rabbits led to a considerable lag in weight gain for the skeletal bones, reduction in Ca45 uptake, and an increase in isotope resorption rate in the rapidly metabolized fraction of extremity bones. On the other hand, Ca45 content in the teeth and maxillae increased, which may be explained by redistribution of isotope among the various mineralized tissues. Injection of thyrocalcitonin (50 IU/day) produced a distinct normalizing effect on Ca45 uptake and resorption in the mineralized tissues of rabbits kept hypokinetic.

Volozhin, A. I.↗
NASA NTRS1980

Cyclic AMP system in muscle tissue during prolonged hypokinesia

Components of the cyclic Adenosine-cyclic-35-monophosphate (AMP) system in the muscle tissue of white rats were studied during 70-75 days of hypokinesia, created by placing the animals in small booths which restricted their movements, and during the readaptation period. In the initial period, cyclic AMP levels and the activities of phosphodiesterase and adenylate cyclase in muscle tissue were increased. The values for these indices were roughly equal for controls and experimental animals during the adaptation period, but on the 70th day of the experiment cAMP levels dropped, phosphodiesterase activity increased, and the stimulative effect of epinephrine on the activity of adenylate cyclase decreased. The indices under study normalized during the readaptation period.

Antipenko, Y. A.↗
NASA NTRS1979

Quantitation of tissue loss during prolonged space flight

Data from Skylab missions related to tissue loss in space were analyzed. Significant changes in gross body composition occur during spaceflight, these include: alterations in water balance resulting from headward shifts of fluid, loss of musculoskeletal tissue, and alterations in fat depending upon the balance between caloric intake and energy expenditure. An effort was made to control the more essential components of body weight on the Skylab missions.

Leonard, J. I.↗
NASA NTRS1982

Determination of oxygen tension in the subcutaneous tissue of cosmonauts during the Salyut-6 mission

A polarographic technique was used to measure the oxygen tension in subcutaneous tissue of the forearm of a cosmonaut prior to, after, and on the fourth day of a space mission performed by Salut-6. A drop in the oxygen exchange rate in the peripheral tissues during weightlessness was observed. The mechanisms of this change are studied, taking into consideration the blood distribution in the organism and microcirculation disorders reflected by a decreased blood flow rate in arterial-venous junctions.

Baranski, S.↗
NASA NTRS1982

Velocity and attenuation of sound in arterial tissues

The velocity of sound in excised human and canine arterial tissues is measured in order to serve as a basis for the development and application of ultrasonic techniques for the diagnosis of atherosclerotic lesions. Measurements of sound velocity at different regions of 11 human and six canine aortas were made by a time delay spectrometer technique at frequencies from 2 to 10 MHz, and compared with ultrasonic attenuation parameters and the results of biochemical assays. Sound velocity is found to increase with increasing attenuation at all frequencies, and with increasing collagen content. A strong dependence of sound velocity on cholesterol content or low calcium contents is not observed, although velocities of up to 2000 m/sec are observed in highly organized calcified lesions. A decrease in velocity with decreasing temperature is also noted. It is thus concluded that it is principally the differences in tissue collagen levels that contribute to image formation according to sound velocity.

Rooney, J. A.↗
NASA NTRS1985

The effects of prostaglandin E2 in growing rats - Increased metaphyseal hard tissue and cortico-endosteal bone formation

The role of in vivo prostaglandin E2 (PGE2) in bone formation is investigated. Twenty-five male Sprague-Dawley rats weighing between 223-267 g were injected subcutaneously with 0.3, 1.0, 3.0, and 6.0 mg of PGE2-kg daily for 21 days. The processing of the tibiae for observation is described. Radiographs and histomorphometric analyses are also utilized to study bone formation. Body weight, weights of soft tissues and bones morphometry are evaluated. It is observed that PGE2 depressed longitudinal bone growth, increased growth cartilage thickness, decreased degenerative cartilage cell size and cartilage cell production, and significantly increased proximal tibial metaphyseal hard tissue mass. The data reveal that periosteal bone formation is slowed down at higher doses of PGE2 and endosteal bone formation is slightly depressed less than 10 days post injection; however, here is a late increase (10 days after post injection) in endosteal bone formation and in the formation of trabecular bone in the marrow cavity of the tibial shaft. It is noted that the effects of PGE2 on bone formation are similar to the responses of weaning rats to PGE2.

Jee, W. S. S.↗
NASA NTRS1987

Shear and mixing effects on cells in agitated microcarrier tissue culture reactors

Tissue cells are known to be sensitive to mechanical stresses imposed on them by agitation in bioreactors. The amount of agitation provided in a microcarrier or suspension bioreactor should be only enough to provide effective homogeneity. Three distinct flow regions can be identified in the reactor: bulk turbulent flow, bulk laminar flow and boundary-layer flows. Possible mechanisms of cell damage are examined by analyzing the motion of microcarriers or free cells relative to the surrounding fluid, to each other and to moving or stationary solid surfaces. The primary mechanisms of cell damage appear to result from: (1) direct interaction between microcarriers and turbulent eddies; (2) collisions between microcarriers in turbulent flow; and (3) collisions against the impeller or other stationary surfaces. If the smallest eddies of turbulent flow are of the same size as the microcarrier beads, they may cause high shear stresses on the cells. Eddies the size of the average interbead spacing may cause bead-bead collisions which damage cells. The severity of the collisions increases when the eddies are also of the same size as the beads. Impeller collisions occur when beads cannot avoid the impeller leading edge as it advances through the liquid. The implications of the results of this analysis on the design and operation of tissue culture reactors are discussed.

Cherry, Robert S.↗
NASA NTRS1990

Insulin and insulin-like growth factor-1 induce pronounced hypertrophy of skeletal myofibers in tissue culture

Skeletal myofibers differentiated from primary avian myoblasts in tissue culture can be maintained in positive nitrogen balance in a serum-free medium for at least 6 to 7 days when embedded in a three dimensional collagen gel matrix. The myofibers are metabolically sensitive to physiological concentrations of insulin but these concentrations do not stimulate cell growth. Higher insulin concentrations stimulate both cell hyperplasia and myofiber hypertrophy. Cell growth results from a long term 42 percent increase in total protein synthesis and a 38 percent increase in protein degradation. Myofiber diameters increase by 71 to 98 percent after 6 to 7 days in insulin-containing medium. Insulin-like growth factor-1 but not insulin-like growth factor-2, at 250 ng/ml, is as effective as insulin in stimulating cell hyperplasia and myofiber hypertrophy. This model system provides a new method for studying the long-term anabolic effects of insulin and insulin-like growth factors on myofiber hypertrophy under defined tissue culture conditions.

Vandenburgh, Herman H.↗
NASA NTRS1989

Tissue fluid pressures - From basic research tools to clinical applications

This paper describes clinical applications of two basic research tools developed and refined in the past 20 years: the wick catheter (for measuring tissue fluid pressure) and the colloid osmometer (for measuring osmotic pressure). Applications of the osmometer include estimations of the reduced osmotic pressure of sickle-cell hemoglobin with deoxygenation, and of reduced swelling pressure of human nucleus pulposus with hydration or upon action of certain enzymes. Clinical uses of the wick-catheter technique include an improvement of diagnosis and treatment of acute and chronic compartment syndromes, the elucidation of the tissue pressure thresholds for neuromuscular dysfunction, and the development of a better tourniquet for orthopedics.

Hargens, Alan R.↗
NASA NTRS1991

Analytical relationships of nuclear field and microdosimetric quantities for target fragmentation in tissue systems

A simple analytic formula for the nuclear fields formed by target fragmentation in tissue systems is derived using the continuous slowing down approximation (CSDA). The energy fluctuations in sensitive localized sites within the tissue system caused by these nuclear events are defined by microdosimetry. In that CSDA is used, the energy fluctuations exclude the role of secondary electrons. The relations also relate to the response of microdosimetric devices to nuclear fragmentation fields.

Wilson, John W.↗