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At least 199 records · Page 11

Use of Tardigrade Proteins for Enhancing Cellular Stress Tolerance on the Moon

With sights set on returning a human presence to the Moon and plans to explore even farther beyond, it is imperative we understand the impact of deep space radiation, partial gravity, and the lunar surface on biology. The need for countermeasures to protect future astronauts from the unique conditions of space is ever-pressing and an assessment of bioproduction capabilities in space is a crucial step for sustaining long-term missions. The Lunar Explorer Instrument for space biology Applications (LEIA) aims to investigate the cell’s response to the lunar environment and test strategies for enhancing cellular tolerance on the Moon, utilizing the budding yeast Saccharomyces cerevisiae as a biosensor. S. cerevisiae serves as an excellent model organism due to its widespread use as an analog for human cells, well-characterized set of genetic tools, and ability to survive desiccation. However, these cells must remain desiccated for up to one year prior to launch and the radiation-sensitive strains designed for LEIA will likely be more vulnerable to desiccation effects. In this work, we introduce tardigrade proteins known for their protective mechanisms into potential LEIA strains to test genetic strategies for improving desiccation and radiation tolerance. Tardigrades are extremotolerant animals well-known for their ability to survive in harsh environments. Notably, they can survive extreme desiccation and radiation due to the upregulation of intrinsically disordered proteins (IDPs). It has been shown that integration of these tardigrade IDPs, such as cytosolic abundant heat-soluble (CAHS) proteins, into yeast can confer increased desiccation tolerance in synergy with trehalose. Our ongoing testing and assessment of bioengineered radiation and desiccation tolerance will help us gain insight into optimizing strain design and selection for protecting LEIA’s biological payload as well as genetic engineering strategies for enhancing bioproduction potential for future missions beyond low Earth orbit.

Genetics↗

Use of Tardigrade Proteins for Enhancing Cellular Stress Tolerance on the Moon

With sights set on returning a human presence to the Moon and plans to explore even farther beyond, it is imperative we understand the impact of deep space radiation, partial gravity, and the lunar surface on biology. The need for countermeasures to protect future astronauts from the unique conditions of space is ever-pressing and an assessment of bioproduction capabilities in space is a crucial step for sustaining long-term missions. The Lunar Explorer Instrument for space biology Applications (LEIA) aims to investigate the cell’s response to the lunar environment and test strategies for enhancing cellular tolerance on the Moon, utilizing the budding yeast Saccharomyces cerevisiae as a biosensor. S. cerevisiae serves as an excellent model organism due to its widespread use as an analog for human cells, well-characterized set of genetic tools, and ability to survive desiccation. However, these cells must remain desiccated for up to one year prior to launch and the radiation-sensitive strains designed for LEIA will likely be more vulnerable to desiccation effects. In this work, we introduce tardigrade proteins known for their protective mechanisms into potential LEIA strains to test genetic strategies for improving desiccation and radiation tolerance. Tardigrades are extremotolerant animals well-known for their ability to survive in harsh environments. Notably, they can survive extreme desiccation and radiation due to the upregulation of intrinsically disordered proteins (IDPs). It has been shown that integration of these tardigrade IDPs, such as cytosolic abundant heat-soluble (CAHS) proteins, into yeast can confer increased desiccation tolerance in synergy with trehalose. Our ongoing testing and assessment of bioengineered radiation and desiccation tolerance will help us gain insight into optimizing strain design and selection for protecting LEIA’s biological payload as well as genetic engineering strategies for enhancing bioproduction potential for future missions beyond low Earth orbit.

Genetics↗

Model based verification of the Secure Socket Layer (SSL) Protocol for NASA systems

The National Aeronautics and Space Administration (NASA) has tens of thousands of networked computer systems and applications. Software Security vulnerabilities present risks such as lost or corrupted data, information theft, and unavailability of critical systems. These risks represent potentially enormous costs to NASA. The NASA Code Q research initiative 'Reducing Software Security Risk (RSSR) Trough an Integrated Approach' offers formal verification of information technology (IT), through the creation of a Software Security Assessment Instrument (SSAI), to address software security risks.

software security↗

Failure environment analysis tool applications

Understanding risks and avoiding failure are daily concerns for the women and men of NASA. Although NASA's mission propels us to push the limits of technology, and though the risks are considerable, the NASA community has instilled within, the determination to preserve the integrity of the systems upon which our mission and, our employees lives and well-being depend. One of the ways this is being done is by expanding and improving the tools used to perform risk assessment. The Failure Environment Analysis Tool (FEAT) was developed to help engineers and analysts more thoroughly and reliably conduct risk assessment and failure analysis. FEAT accomplishes this by providing answers to questions regarding what might have caused a particular failure; or, conversely, what effect the occurrence of a failure might have on an entire system. Additionally, FEAT can determine what common causes could have resulted in other combinations of failures. FEAT will even help determine the vulnerability of a system to failures, in light of reduced capability. FEAT also is useful in training personnel who must develop an understanding of particular systems. FEAT facilitates training on system behavior, by providing an automated environment in which to conduct 'what-if' evaluation. These types of analyses make FEAT a valuable tool for engineers and operations personnel in the design, analysis, and operation of NASA space systems.

Pack, Ginger L.↗

Failure environment analysis tool applications

Understanding risks and avoiding failure are daily concerns for the women and men of NASA. Although NASA's mission propels us to push the limits of technology, and though the risks are considerable, the NASA community has instilled within it, the determination to preserve the integrity of the systems upon which our mission and, our employees lives and well-being depend. One of the ways this is being done is by expanding and improving the tools used to perform risk assessment. The Failure Environment Analysis Tool (FEAT) was developed to help engineers and analysts more thoroughly and reliably conduct risk assessment and failure analysis. FEAT accomplishes this by providing answers to questions regarding what might have caused a particular failure; or, conversely, what effect the occurrence of a failure might have on an entire system. Additionally, FEAT can determine what common causes could have resulted in other combinations of failures. FEAT will even help determine the vulnerability of a system to failures, in light of reduced capability. FEAT also is useful in training personnel who must develop an understanding of particular systems. FEAT facilitates training on system behavior, by providing an automated environment in which to conduct 'what-if' evaluation. These types of analyses make FEAT a valuable tool for engineers and operations personnel in the design, analysis, and operation of NASA space systems.

Pack, Ginger L.↗

Evaluation of Case Size 0603 BME Ceramic Capacitors

High volumetric efficiency of commercial base metal electrode (BME) ceramic capacitors allows for a substantial reduction of weight and sizes of the parts compared to currently used military grade precious metal electrode (PME) capacitors. Insertion of BME capacitors in space applications requires a thorough analysis of their performance and reliability. In this work, six types of cases size 0603 BME capacitors from three vendors have been evaluated. Three types of multilayer ceramic capacitors (MLCCs) were designed for automotive industry and three types for general purposes. Leakage currents in the capacitors have been measured in a wide range of voltages and temperatures, and measurements of breakdown voltages (VBR) have been used to assess the proportion and severity of defects in the parts. The effect of soldering-related thermal shock stresses was evaluated by analysis of distributions of VBR for parts in 'as is' condition and after terminal solder dip testing at 350 C. Highly Accelerated Life Testing (HALT) at different temperatures was used to assess the activation energy of degradation of leakage currents and predict behavior of the parts at life test and normal operating conditions. To address issues related to rework and manual soldering, capacitors were soldered onto different substrates at different soldering conditions. The results show that contrary to a common assumption that large-size capacitors are mostly vulnerable to soldering stresses, cracking in small size capacitors does happen unless special measures are taken during assembly processes.

testing↗

Preliminary Investigation of Impact of Technological Impairment on Trajectory-Based Operations

The Next Generation Air Transportation System (NextGen) incorporates collaborative air traffic management and Trajectory-Based Operations (TBO) in order to significantly increase the capacity, efficiency, and predictability of operations in the National Airspace System (NAS), without decreasing safety. This is enabled by airspace users and service providers sharing knowledge about operations that allows prediction of the complete 4D flight trajectory with as little uncertainty as possible. Additionally, new software and hardware technology is critical to reaching NextGen goals, especially with regard to TBO. What if the technologies that are critical for TBO were to be impaired or fail completely? Should there be a malfunction of a piece of the technology, it must be ensured that the whole system does not break down completely or suffer severe impairment. Instead, operations need to be maintained proportionally to the problem and safety needs to be ensured (graceful degradation). This paper proposes a systematic framework to investigate the vulnerability of TBO to technology disruption, and determine the impact of technological impairment on TBO. Two representative technologies are chosen for detailed investigation and the impact of their impairment on the degradation of TBO is illustrated using a weather-related scenario. XXXX There are several possible directions of future work. We believe it is desirable to develop methods to quantitatively assess the impact of technological disruption on TBO and to have the simulation tools to validate the impact. The availability of prognostics and health management methods could be leveraged to predict technological failure/disruption, thus predicting how TBO will be a ected, and possibly pro-actively mitigating the impact. It is important to develop large-scale scenarios where the e ect of technological impairment is prominent, and identify methods to quantitatively assess the extent of TBO degradation. An important goal of such an investigation is the development of failure-resistant resilient trajectory-based oper- ations. Resilience14, 15 is the property of a system to \bounce back" and resume at least a signi cant portion of its functionalities after degradation due to technological impairment(s). A systems resilience includes properties such as \bu ering capacity" (quantifying disruptions the system can absorb or adapt to without a fundamental breakdown in performance or in the systems structure), \ exibility" (ability to restructure itself in response to external changes or pressures), "margin" (how closely the system is currently operating rela- tive to one or another kind of performance boundary), \tolerance" (whether the system gracefully degrades as stress/pressure increase, or collapses quickly when pressure exceeds adaptive capacity), etc. Future work needs to focus on quantifying and improving the resilience of TBO, and identifying resilient design solutions for aviation.

TBO↗

Exposure of the EU-28 Food Imports to Extreme Weather Disasters in Exporting Countries

EU-28 relies on a diversified foreign market, even for crops for which it has a high self-sufficiency.This study contributes to the discussion on the vulnerability of agri-food supply to the impacts of extreme weather disasters (EWD). We focus on the largest import commodities of the EU-28 and we aim to (1) map external dependencies of EU-28 agri-food sector, (2) estimate the impact of EWD on crop production in countries from which the EU-28 receives their imports, and (3) assess the exposure of EU-28 agri-food imports to such impacts. Crop and trade data areacquired through EUROSTAT and FAOSTAT, EWD records from EM-DAT, all between 1961 and 2016. A superposed epoch analysis is used to estimate the impact of EWD on the average national production, yield and harvested area of selected crops in exporting countries. The EU-28 imports between 35-100% of its consumption of soybeans, banana, tropical fruits, coffee and cocoa. Our study reveals a substantial impact of EWD, especially due to droughts andheat waves, on the production of soybeans, tropical fruits, and cocoa, with import weighted impacts of 3, 8, and 7%, respectively. Floods cause weighted impacts of 7% (soybeans) and 8% (tropical fruits). Coffee production shows gains during cold waves, but the inter-annual variability offsets these effects.

Teresa Armada Bras↗

The Parallel System for Integrating Impact Models and Sectors (pSIMS)

We present a framework for massively parallel climate impact simulations: the parallel System for Integrating Impact Models and Sectors (pSIMS). This framework comprises a) tools for ingesting and converting large amounts of data to a versatile datatype based on a common geospatial grid; b) tools for translating this datatype into custom formats for site-based models; c) a scalable parallel framework for performing large ensemble simulations, using any one of a number of different impacts models, on clusters, supercomputers, distributed grids, or clouds; d) tools and data standards for reformatting outputs to common datatypes for analysis and visualization; and e) methodologies for aggregating these datatypes to arbitrary spatial scales such as administrative and environmental demarcations. By automating many time-consuming and error-prone aspects of large-scale climate impacts studies, pSIMS accelerates computational research, encourages model intercomparison, and enhances reproducibility of simulation results. We present the pSIMS design and use example assessments to demonstrate its multi-model, multi-scale, and multi-sector versatility.

crop modeling↗

Sacramento Urban Development: Quantifying and Mapping Urban Heat to Inform Urban Planning Initiatives in Sacramento, California

The combined effects of increasing urbanization and changing climatic conditions have exacerbated the urban heat island (UHI) effect and heat-related risks for city dwellers. Vulnerability to heat-related illnesses is further compounded by risk factors such as demographics, socioeconomic status, and pre-existing health conditions. The City of Sacramento, as California’s fastest-growing city by population, is particularly invested in combatting the UHI effect. The team collaborated with the City of Sacramento and urban planning firm, Dyett and Bhatia, on three main goals: assessing urban heat at the neighborhood scale, identifying priority areas for cooling interventions, and assessing heat risk to the population. This project utilized NASA Earth observation products to identify hotspots within the communities of Sacramento and create maps of urban heat, the heat-mitigation index, and heat risk from 2016-2020. The team used the Surface Reflectance product from Landsat 8 Operational Land Imager (OLI) and Thermal Infrared Sensor (TIRS) and the ECOsystem Spaceborne Thermal Radiometer Experiment on Space Station (ECOSTRESS) thermal infrared sensor. Additionally, the Integrated Valuation of Ecosystem Services and Tradeoffs (InVEST) urban cooling model was used to assess the impact of increased tree canopy scenarios. Urban hotspots were identified in central Sacramento and along major transportation corridors such as Stockton Boulevard, while the highest risk areas were identified in the communities of Fruitridge/Broadway and North Sacramento. This project identified these high-opportunity areas for heat mitigation to inform the City of Sacramento's General Plan and reduce citizen risk by addressing urban heat islands.

DEVELOP Project Summary↗

Sacramento Urban Development: Quantifying and Mapping Urban Heat to Support Urban Planning Initiatives in Sacramento, California

The combined effects of increasing urbanization and climate change have exacerbated the urban heat island (UHI) effect and heat-related risks for city dwellers. Vulnerability to heat-related illnesses is further compounded by risk factors such as demographics, socioeconomic status, and pre-existing health conditions. The City of Sacramento, as California’s fastest growing city in terms of population, is particularly invested in combatting the UHI effect. The team collaborated with the City of Sacramento and urban planning firm, Dyett and Bhatia, on three main goals: assessing urban heat at the neighborhood scale; identifying priority areas for cooling interventions; and assessing heat risk to the population. This project utilized NASA Earth observation products to identify hotspots within the community areas of Sacramento and create maps of urban heat, the heat-mitigation index, and heat risk of the study period from 2016-2020. The Surface Reflectance product were used from Landsat 8 Operational Land Imager (OLI) and Thermal Infrared Sensor (TIRS) and ECOsystem Spaceborne Thermal Radiometer Experiment on Space Station (ECOSTRESS) thermal infrared sensor. Additionally, the Integrated Valuation of Ecosystem Services and Tradeoffs (InVEST) Urban cooling model was used to assess the impact of increased tree canopy scenarios. Urban hotspots were identified in central Sacramento and along major transportation corridors such as Stockton Boulevard, while highest risk areas were identified in the community areas of Fruitridge/Broadway and North Sacramento. This project identified these high-opportunity areas for heat mitigation to inform the City of Sacramento's General Plan. This will inform the partners’ plans to reduce citizen risk by addressing urban heat islands.

Karina Alvarez↗

Status of research into lightning effects on aircraft

Developments in aircraft lightning protection since 1938 are reviewed. Potential lightning problems resulting from present trends toward the use of electronic controls and composite structures are discussed, along with presently available lightning test procedures for problem assessment. The validity of some procedures is being questioned because of pessimistic results and design implications. An in-flight measurement program is needed to provide statistics on lightning severity at flight altitudes and to enable more realistic tests, and operators are urged to supply researchers with more details on electronic components damaged by lightning strikes. A need for review of certain aspects of fuel system vulnerability is indicated by several recent accidents, and specific areas for examination are identified. New educational materials and standardization activities are also noted.

Plumer, J. A.↗

Physical, Ecological, and Societal Indicators for the National Climate Assessment

The National Climate Assessment (NCA) is being conducted under the auspices of the U.S. Global Change Research Program (USGCRP), pursuant to the Global Change Research Act of 1990, Section 106, which requires a report to Congress every 4 years. The current NCA (http://globalchange.gov/what-we-do/assessment/) differs in multiple ways from previous U.S. climate assessment efforts, being: (1) more focused on supporting the Nation s activities in adaptation and mitigation and on evaluating the current state of scientific knowledge relative to climate impacts and trends; (2) a long-term, consistent process for evaluation of climate risks and opportunities and providing information to support decision-making processes within regions and sectors; and (3) establishing a permanent assessment capacity both inside and outside of the federal government. As a part of ongoing, long-term assessment activities, the NCA intends to develop an integrated strategic framework and deploy climate-relevant physical, ecological, and societal indicators. The NCA indicators framework is underdevelopment by the NCA Development and Advisory Committee Indicators Working Group and are envisioned as a relatively small number of policy-relevant integrated indicators designed to provide a consistent, objective, and transparent overview of major variations in climate impacts, vulnerabilities, adaptation, and mitigation activities across sectors, regions, and timeframes. The potential questions that could be addressed by these indicators include: How do we know that there is a changing climate and how is it expected to change in the future? Are important climate impacts and opportunities occurring or predicted to occur in the future? Are we adapting successfully? What are the vulnerabilities and resiliencies given a changing climate? Are we preparing adequately for extreme events? It is not expected that the NCA societal indicators would be linked directly to a single decision or portfolio of decisions, but subsets of indicators, or the data supporting the indicator, might be used to inform decision-making processes such as the development and implementation of climate adaptation strategies in a particular sector or region.

Kenney, Melissa A.↗

Debris environment interactions with low Earth orbit constellations

Several low earth orbit communication satellite constellations are planned. Due to their size and complexity, these constellations potentially contribute to the orbital debris environment. The results of a parametric assessment of the impact of low earth orbit constellations on the orbital debris environment are presented. The increase in loss rate of non constellation spacecraft is considered, as well as the increase in the loss rate or replacement rate of constellation satellites as a result of debris impact. Primary parameters in the analysis are the number, size and altitude of the constellation. Parameters are defined for the vulnerable area of loss of spacecraft and the disposition of constellation spacecraft at the end of its life.

Reynolds, Robert↗

Radiation effect on rocket engine performance

The effects of radiation on the performance of modern rocket propulsion systems operating at high pressure and temperature were recognized as a key issue in the design and operation of various liquid rocket engines of the current and future generations. Critical problem areas of radiation coupled with combustion of bipropellants are assessed and accounted for in the formulation of a universal scaling law incorporated with a radiation-enhanced vaporization combustion model. Numerical algorithms are developed and the pertaining data of the Variable Thrust Engine (VTE) and Space Shuttle Main Engine (SSME) are used to conduct parametric sensitivity studies to predict the principal intercoupling effects of radiation. The analysis reveals that low enthalpy engines, such as the VTE, are vulnerable to a substantial performance set back by the radiative loss, whereas the performance of high enthalpy engines such as the SSME, are hardly affected over a broad range of engine operation. Additionally, combustion enhancement by the radiative heating of the propellant has a significant impact in those propellants with high absorptivity. Finally, the areas of research related with radiation phenomena in bipropellant engines are identified.

Chiu, Huei-Huang↗

Productivity issues at organizational interfaces

The need for close interdependence between large numbers of diverse and specialized work groups makes the Space Program extremely vulnerable to loss of productivity at organizational interfaces. Trends within the program also suggest that the number and diversity of interfaces will grow in the near term. Continued maintenance of R&D excellence will require that interface performance issues be included in any future productivity improvement effort. The types and characteristics of organizational interfaces are briefly presented, followed by a review of factors which impact their productivity. Approaches to assessing and improving interface effectiveness are also discussed.

Holland, A. W.↗

The Hurricane-Flood-Landslide Continuum

In August 2004, representatives from NOAA, NASA, the USGS, and other government agencies convened in San Juan, Puerto Rim for a workshop to discuss a proposed research project called the Hurricane-Flood-Landslide Continuum (HFLC). The essence of the HFLC is to develop and integrate tools across disciplines to enable the issuance of regional guidance products for floods and landslides associated with major tropical rain systems, with sufficient lead time that local emergency managers can protect vulnerable populations and infrastructure. All three lead agencies are independently developing precipitation-flood-debris flow forecasting technologies, and all have a history of work on natural hazards both domestically and overseas. NOM has the capability to provide tracking and prediction of storm rainfall, trajectory and landfall and is developing flood probability and magnTtude capabilities. The USGS has the capability to evaluate the ambient stability of natural and man-made landforms, to assess landslide susceptibilities for those landforms, and to establish probabilities for initiation of landslides and debris flows. Additionally, the USGS has well-developed operational capacity for real-time monitoring and reporting of streamflow across distributed networks of automated gaging stations (http://water.usgs.gov/waterwatch/). NASA has the capability to provide sophisticated algorithms for satellite remote sensing of precipitation, land use, and in the future, soil moisture. The Workshop sought to initiate discussion among three agencies regarding their specific and highly complimentary capabilities. The fundamental goal of the Workshop was to establish a framework that will leverage the strengths of each agency. Once a prototype system is developed for example, in relatively data-rich Puerto Rim, it could be adapted for use in data-poor, low-infrastructure regions such as the Dominican Republic or Haiti. This paper provides an overview of the Workshop s goals, presentations and recommendations with respect to the development of the HFLC.

Negri, Andrew J.↗

Assessment of the NASA Space Shuttle Program's Problem Reporting and Corrective Action System

This paper documents the general findings and recommendations of the Design for Safety Programs Study of the Space Shuttle Programs (SSP) Problem Reporting and Corrective Action (PRACA) System. The goals of this Study were: to evaluate and quantify the technical aspects of the SSP's PRACA systems, and to recommend enhancements addressing specific deficiencies in preparation for future system upgrades. The Study determined that the extant SSP PRACA systems accomplished a project level support capability through the use of a large pool of domain experts and a variety of distributed formal and informal database systems. This operational model is vulnerable to staff turnover and loss of the vast corporate knowledge that is not currently being captured by the PRACA system. A need for a Program-level PRACA system providing improved insight, unification, knowledge capture, and collaborative tools was defined in this study.

Korsmeryer, D. J.↗