Martian cratering.
Martian craters, crater analysis techniques, impact velocities, etc, compared to lunar cratering and possibility of biological existence from Mariner IV photographs
MARINER IV SPACE PROBE↗
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Results for “biological techniques”
Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.
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Physiological factors and optical parameters as bases of vegetation discrimination and stress analysis.
The successful application of photographic, optical-mechanical scanning, and other sensing techniques to vegetation must be based on an understanding of the biological and physical principles involved. The objective is to review the biological and optical characteristics of vegetation and to discuss how knowledge of them can support operational programs by aiding in wavelength selection, timing of missions, and discriminations between vegetation types as well as between healthy and stressed plants of the same type. Both laboratory situations, in which relatively pure spectral data are obtained, and field situations are considered. In the latter, sensor performance and background variables affect data quality.
Wiegand, C. L.↗
New horizons for study of the cardiopulmonary and circulatory systems
The paper discusses the development of computer-controlled three-dimensional reconstruction techniques designed to determine the dynamic changes in the true shape and dimensions of the epi- and endocardial surfaces of the heart, along with variable time base (stop-action to real-time) displays of the transmural distribution of the coronary microcirculation and the three-dimensional anatomy of the macrovasculature in all regions of the body throughout individual cardiac and/or respiratory cycles. A technique for reconstructing a cross section of the heart from multiplanar videoroentgenograms is outlined. The capability of high spatial and high temporal resolution scanning videodensitometry makes possible measurement of the appearance, mean transit and clearance of roentgen opaque substances in three-dimensional space through the myocardium with a degree of simultaneous anatomic and temporal resolution not obtainable by current isotope techniques. The distribution of a variety of selected chemical elements or biologic materials within a body portion can also be determined.
Wood, E. H.↗
Ultrasonic frequency analysis
Technique is used for evaluation and characterization of materials, fluids, and biological tissue. Method eliminates problem of electrical drive pulse shape by slaving tracking generator to local oscillator of spectrum analyzer. Logic/timing generator is used to control pulse transmission and receiving sequence, pulse width, and pulse repetition rate.
Cantrell, J. H.↗
Determining D/L Ratios of Amino Acids Found in Ice Above Lake Vostok Using ESI/CIT Mass Spectroscopy
Astrobiology is an area where longevity of (micro) organisms is of great interest. Cryospheres are common phenomena in the solar system, particularly on satellites, comets and asteroids, as well as at least some of the planets. Recent data from the Mars Global Surveyor mission suggest the possibility of permafrost or perhaps even liquid water under the Martian surface [2]. These environments may be the areas in which the probability of finding life is the highest. This issue is of concern due to the probable evolution of planetary environments such as that of Mars from more hospitable to less hospitable conditions over the history of the solar system. In addition, evaluation of the possible transfer of living organisms between planets via impact ejecta [3] is dependent on knowledge of the maximum time periods over which microorganisms can remain dormant and subsequently revive and reproduce.Amino acid racemization dating, or aminostratigraphy, has been used for many years to date biological systems, and has been examined as a possible biosignature detection technique for Mars. We have suggested using amino acid racemization as one of the most indicative biosignatures [4]. Only life systems produce preferential synthesis of L-amino acids versus D-amino acids. Almost all amino acids in terrestrial organisms can be found only in the L-enantiomeric form.We studied the level of amino acid racemization, specifically of aspartic acid, in permafrost samples from eastern Siberia. Also we analyzed samples of ice from borehole drilled to lake Vostok, Antarctica.
A Tsapin↗
Regolith Activation on the Lunar Surface and its Ground Test Simulation
Activation of the surfaces of lunar regolith particles can occur through interactions with solar electromagnetic radiation, solar and galactic particle radiation and micrometeoroid bombardment. An attempt has been made to quantify the relative importance of each of those effects. The effects of these activated surfaces may be to enhance the adhesion and toxicity of the particles. Also key to the importance of activation is the lifetimes of activated states in various environments which is controlled by their passivation rate as well as their activation rate. Although techniques exist to characterize the extent of activation of particles in biological system, it is important to be able to quantify the activation state on the lunar surface, in ground-test vacuum systems, and in habitat atmospheres as well.
Gaier, James R.↗
Regolith Activation on the Lunar Surface and Its Ground Test Simulation
Activation of the surfaces of lunar regolith particles can occur through interactions with solar electromagnetic radiation, solar and galactic particle radiation and micrometeoroid bombardment. An attempt has been made to quantify the relative importance of each of those effects. The effects of these activated surfaces may be to enhance the adhesion and toxicity of the particles. Also key to the importance of activation is the lifetimes of activated states in various environments which is controlled by their passivation rate as well as their activation rate. Although techniques exist to characterize the extent of activation of particles in biological system, it is important to be able to quantify the activation state on the lunar surface, in ground-test vacuum systems, and in habitat atmospheres as well.
Gaier, James R.↗
Radiation Protection Using Carbon Nanotube Derivatives
BHA and BHT are well-known food preservatives that are excellent radical scavengers. These compounds, attached to single-walled carbon nanotubes (SWNTs), could serve as excellent radical traps. The amino-BHT groups can be associated with SWNTs that have carbolyxic acid groups via acid-base association or via covalent association. The material can be used as a means of radiation protection or cellular stress mitigation via a sequence of quenching radical species using nano-engineered scaffolds of SWNTs and their derivatives. It works by reducing the number of free radicals within or nearby a cell, tissue, organ, or living organism. This reduces the risk of damage to DNA and other cellular components that can lead to chronic and/or acute pathologies, including (but not limited to) cancer, cardiovascular disease, immuno-suppression, and disorders of the central nervous system. These derivatives can show an unusually high scavenging ability, which could prove efficacious in protecting living systems from radical-induced decay. This technique could be used to protect healthy cells in a living biological system from the effects of radiation therapy. It could also be used as a prophylactic or antidote for radiation exposure due to accidental, terrorist, or wartime use of radiation- containing weapons; high-altitude or space travel (where radiation exposure is generally higher than desired); or in any scenario where exposure to radiation is expected or anticipated. This invention s ultimate use will be dependent on the utility in an overall biological system where many levels of toxicity have to be evaluated. This can only be assessed at a later stage. In vitro toxicity will first be assessed, followed by in vivo non-mammalian screening in zebra fish for toxicity and therapeutic efficacy.
Conyers, Jodie L., Jr.↗
Life Sciences Division Spaceflight Hardware
The Ames Research Center (ARC) is responsible for the development, integration, and operation of non-human life sciences payloads in support of NASA's Gravitational Biology and Ecology (GB&E) program. To help stimulate discussion and interest in the development and application of novel technologies for incorporation within non-human life sciences experiment systems, three hardware system models will be displayed with associated graphics/text explanations. First, an Animal Enclosure Model (AEM) will be shown to communicate the nature and types of constraints physiological researchers must deal with during manned space flight experiments using rodent specimens. Second, a model of the Modular Cultivation System (MCS) under development by ESA will be presented to highlight technologies that may benefit cell-based research, including advanced imaging technologies. Finally, subsystems of the Cell Culture Unit (CCU) in development by ARC will also be shown. A discussion will be provided on candidate technology requirements in the areas of specimen environmental control, biotelemetry, telescience and telerobotics, and in situ analytical techniques and imaging. In addition, an overview of the Center for Gravitational Biology Research facilities will be provided.
Yost, B.↗
Inelastic electron tunneling spectroscopy
Inelastic electron tunneling spectroscopy is a useful technique for the study of vibrational modes of molecules adsorbed on the surface of oxide layers in a metal-insulator-metal tunnel junction. The technique involves studying the effects of adsorbed molecules on the tunneling spectrum of such junctions. The data give useful information about the structure, bonding, and orientation of adsorbed molecules. One of the major advantages of inelastic electron tunneling spectroscopy is its sensitivity. It is capable of detecting on the order of 10 to the 10th molecules (a fraction of a monolayer) on a 1 sq mm junction. It has been successfully used in studies of catalysis, biology, trace impurity detection, and electronic excitations. Because of its high sensitivity, this technique shows great promise in the area of solid-state electronic chemical sensing.
Khanna, S. K.↗
Neuroprotective "agents" in surgery. Secret "agent" man, or common "agent" machine?
The search for clinically-effective neuroprotective agents has received enormous support in recent years--an estimated $200 million by pharmaceutical companies on clinical trials for traumatic brain injury alone. At the same time, the pathophysiology of brain injury has proved increasingly complex, rendering the likelihood of a single agent "magic bullet" even more remote. On the other hand, great progress continues with technology that makes surgery less invasive and less risky. One example is the application of endovascular techniques to treat coronary artery stenosis, where both the invasiveness of sternotomy and the significant neurological complication rate (due to microemboli showering the cerebral vasculature) can be eliminated. In this paper we review aspects of intraoperative neuroprotection both present and future. Explanations for the slow progress on pharmacologic neuroprotection during surgery are presented. Examples of technical advances that have had great impact on neuroprotection during surgery are given both from coronary artery stenosis surgery and from surgery for Parkinson's disease. To date, the progress in neuroprotection resulting from such technical advances is an order of magnitude greater than that resulting from pharmacologic agents used during surgery. The progress over the last 20 years in guidance during surgery (CT and MRI image-guidance) and in surgical access (endoscopic and endovascular techniques) will soon be complemented by advances in our ability to evaluate biological tissue intraoperatively in real-time. As an example of such technology, the NASA Smart Probe project is considered. In the long run (i.e., in 10 years or more), pharmacologic "agents" aimed at the complex pathophysiology of nervous system injury in man will be the key to true intraoperative neuroprotection. In the near term, however, it is more likely that mundane "agents" based on computers, microsensors, and microeffectors will be the major impetus to improved intraoperative neuroprotection.
Review Literature↗
The Influence of Shielding on the Biological Effectiveness of Accelerated Particles for the Induction of Chromosome Damages
Chromosome damage was assessed in human peripheral blood lymphocytes after in vitro exposure to the either Si-28 (490 or 600 MeV/n), Ti-48 (1000 MeV/n), or Fe-56 (600, 1000, or 5000 MeV/n). LET values for these ions ranged from approximately 50 to 174 keV/micrometers and doses ranged from 10 to 200 cGy. The effect of either aluminum or polyethylene shielding on the induction of chromosome aberrations was investigated for each ion. Chromosome exchanges were measured using fluorescence in situ hybridization (FISH) with whole chromosome probes in cells collected 48-56 hours after irradiation using a chemical-induced premature chromosome condensation (PCC) technique. The yield of chromosomal aberrations increased linearly with dose and the relative biological effectiveness (RBE) for the primary beams, estimated from the initial slope of the dose response curve for total chromosomal exchanges with respect to gamma-rays, ranged from 14 to 35. The RBE values increased with LET, reaching a maximum for the 1 GeV/n Fe ions with LET of 150 keV/micrometers, and decreased with further increases in LET. When LET of the primary beam was in the region of increasing RBE (i.e. below approximately 100 keV/micrometers), the addition of shielding material increased the effectiveness per unit dose. Whereas shielding decreased the effectiveness per unit dose when the LET of the primary particle beam was higher than 150 keV/micrometers.
George, K.↗
Personnel techniques necessary to maximize bio-barrier integrity at a Martian receiving laboratory
The planning of biological isolation measures for the Mars Surface Sample Return Mission is discussed in terms of personnel and organizational management. Deficiencies in past operation of the Lunar Receiving Laborator are analyzed. It was found that the failure to clearly define relationship among the government agencies involved and to effectively integrate their objectives and responsibilities was a major cause of Laboratory deficiencies. Possible solutions to these problems are presented for application to future missions.
Michaelson, G. S.↗
Detection of Objects Hidden in Highly Scattering Media Using Time-Gated Imaging Methods
Non-intrusive and non-invasive optical imaging techniques has generated great interest among researchers for their potential applications to biological study, device characterization, surface defect detection, and jet fuel dynamics. Non-linear optical parametric amplification gate (NLOPG) has been used to detect back-scattered images of objects hidden in diluted Intralipid solutions. To directly detect objects hidden in highly scattering media, the diffusive component of light needs to be sorted out from early arrived ballistic and snake photons. In an optical imaging system, images are collected in transmission or back-scattered geometry. The early arrival photons in the transmission approach, always carry the direct information of the hidden object embedded in the turbid medium. In the back-scattered approach, the result is not so forth coming. In the presence of a scattering host, the first arrival photons in back-scattered approach will be directly photons from the host material. In the presentation, NLOPG was applied to acquire time resolved back-scattered images under the phase matching condition. A time-gated amplified signal was obtained through this NLOPG process. The system's gain was approximately 100 times. The time-gate was achieved through phase matching condition where only coherent photons retain their phase. As a result, the diffusive photons, which were the primary contributor to the background, were removed. With a large dynamic range and high resolution, time-gated early light imaging has the potential for improving rocket/aircraft design by determining jets shape and particle sizes. Refinements to these techniques may enable drop size measurements in the highly scattering, optically dense region of multi-element rocket injectors. These types of measurements should greatly enhance the design of stable, and higher performing rocket engines.
Galland, Pierre A.↗
Transphoresis and isotachophoresis as preparative techniques with reference to zero-gravity
Transphoresis and isotachophoresis are high resolution electrophoretic separation methods for biological materials. They benefit substantially from a weightless environment. Low power consumption and apparatus payload per unit product yield reflect their high electrophoretic efficiency. Properties unique to these systems, including their imposition of self-stabilizing geometry, suggest novel apparatus automatically controllable by largely electrical means with few or no moving parts. Configurations allowing continuous and semicontinuous operation, and further improvement of efficiency, are discussed.
Hinckley, J. O. N.↗
Prolonged recording from single vestibular units in the frog during plane and space flight, its significance and technique.
Prolonged recording from single vestibular units in frog during plane and space flight, significance and technique
GRAVITATIONAL EFFECT↗
The effects of estimation of censoring, truncation, transformation and partial data vectors
The purpose of this research was to attack statistical problems concerning the estimation of distributions for purposes of predicting and measuring assembly performance as it appears in biological and physical situations. Various statistical procedures were proposed to attack problems of this sort, that is, to produce the statistical distributions of the outcomes of biological and physical situations which, employ characteristics measured on constituent parts. The techniques are described.
Hartley, H. O.↗
Biologically controlled minerals as potential indicators of life
Minerals can be produced and deposited either by abiotic or biologic means. Regardless of their origin, mineral crystals reflect the environment conditions (e.g., temperature, pressure, chemical composition, and redox potential) present during crystal formation. Biologically-produced mineral crystals are grown or reworked under the control of their host organism and reflect an environment different from the abiotic environment. In addition, minerals of either biologic or abiotic origin have great longevities. For these reasons, biologically produced minerals have been proposed as biomarkers. Biomarkers are key morphological, chemical, and isotopic signatures of living systems that can be used to determine if life processes have occurred. Studies of biologically controlled minerals produced by the protist, Paramecium tetraurelia, were initiated since techniques have already been developed to culture them and isolate their crystalline material, and methods are already in place to analyze this material. Two direct crystalline phases were identified. One phase, whose chemical composition is high in Mg, was identified as struvite. The second phase, whose chemical composition is high in Ca, has not been previously found occurring naturally and may be considered a newly discovered material. Analyses are underway to determine the characteristics of these minerals in order to compare them with characteristics of these minerals in order to compare them with characteristics of minerals formed abiotically, but with the same chemical composition.
Schwartz, D. E.↗