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Chondrule rims and interchondrule matrix in UOC

Opaque rims around chondrules and clasts were distinguished from opaque, interchondrule matrix apparently unrelated spatially to chondrules and clasts. Microprobe and electron microscope techniques were used. The mean chemical composition for dark rim and matrix in Bishunpur and Tieschitz are similar to the opaque matrix of Huss, et al. However, the mean dark rim compositions in Bishunpur have significantly higher Fe, and lower Na, K, Al and Si than opaque interchondrule matrix. The opaque matrix of Huss, et al, essentially lies between these compositions. In Tieschitz only rim material was observed. In Tieschitz the rims are Si-poor and dominated by normative olivine (Fo50). Again there is an Na, K, Al component but is often nepheline normative rather than albitic. It too is probably present as glass, Ashworth (pers.comm.). In Bishunpur rims as well as the silicate-FeS, FeNi layering described by Allen, et al, discontinuous layering was observed within the silicate portion. This is apparently due to variations in the proportions of the components, particularly in the glassy phase. In Bishunpur there is a strong genetic link between matrix and rims, although rims seem to have formed under different, possibly more oxidizing, conditions. Also the presence of the same component in rims, matrix chondrules, and clasts suggests a common source.

Alexander, C.↗

Steady-State Characterization of Bacteriorhodopsin-D85N Photocycle

An operational characterization of the photocycle of the genetic mutant D85N of bacteriorhodopsin, BR-D85N, is presented. Steady-state bleach spectra and pump-probe absorbance data are obtained with thick hydrated films containing BR-D85N embedded in a gelatin host. Simple two- and three-state models are used to analyze the photocycle dynamics and extract relevant information such as pure-state absorption spectra, photochemical-transition quantum efficiencies, and thermal lifetimes of dominant states appearing in the photocycle, the knowledge of which should aid in the analysis of optical recording and retrieval of data in films incorporating this photochromic material. The remarkable characteristics of this material and their implications from the viewpoint of optical data storage and processing are discussed.

Timucin, Dogan A.↗

Petrology and petrogenesis of lunar breccia 12013

Sample 12013 is extremely rich in incompatible elements (K, P, U, Th, Pb, Ba, Rb, Zr, Nb, and rare earths) relative to other lunar samples, and it contains abundant granitic material composed of silica minerals and K-feldspar. The present investigation provides detailed petrologic and mineralogic descriptions of the rock and a discussion of its genesis and history. The sample is made up of two lithologies, including a mottled gray and white lithology, and a black lithology. A detailed petrology of the gray breccia is presented, taking into account lithic clasts, mineral clasts, deformation textures in clasts, the matrix, and felsite. A detailed petrology of the black breccia is also provided. Attention is given to a model for the formation of the breccia, an evaluation of the possible genetic relationships between the various components, and some constraints on the nature of the source area.

Quick, J. E.↗

Evolutionary Design of a Robotic Material Defect Detection System

During the post-flight inspection of SSME engines, several inaccessible regions must be disassembled to inspect for defects such as cracks, scratches, gouges, etc. An improvement to the inspection process would be the design and development of very small robots capable of penetrating these inaccessible regions and detecting the defects. The goal of this research was to utilize an evolutionary design approach for the robotic detection of these types of defects. A simulation and visualization tool was developed prior to receiving the hardware as a development test bed. A small, commercial off-the-shelf (COTS) robot was selected from several candidates as the proof of concept robot. The basic approach to detect the defects was to utilize Cadmium Sulfide (CdS) sensors to detect changes in contrast of an illuminated surface. A neural network, optimally designed utilizing a genetic algorithm, was employed to detect the presence of the defects (cracks). By utilization of the COTS robot and US sensors, the research successfully demonstrated that an evolutionarily designed neural network can detect the presence of surface defects.

Ballard, Gary↗

Excess nutrients in hydroponic solutions alter nutrient content of rice, wheat, and potato

Environment has significant effects on the nutrient content of field-grown crop plants. Little is known, however, about compositional changes caused by controlled environments in which plants receive only artificial radiation and soilless, hydroponic culture. This knowledge is essential for developing a safe, nutritious diet in a Controlled Ecological Life-Support System (CELSS). Three crops that are candidates for inclusion in a CELSS (rice, wheat, and white potato) were grown both in the field and in controlled environments where the hydroponic nutrient solution, photosynthetic photon flux (PPF), and CO2 level were manipulated to achieve rapid growth rates. Plants were harvested at maturity, separated into discrete parts, and dried prior to analysis. Plant materials were analyzed for proximate composition (protein, fat, ash, and carbohydrate), total nitrogen (N), nitrate, minerals, and amino-acid composition. The effect of environment on nutrient content varied by crop and plant part. Total N and nonprotein N (NPN) contents of plant biomass generally increased under controlled-environment conditions compared to field conditions, especially for leafy plant parts and roots. Nitrate levels were increased in hydroponically-grown vegetative tissues, but nitrate was excluded from grains and tubers. Mineral content changes in plant tissue included increased phosphorus and decreased levels of certain micronutrient elements under controlled-environment conditions. These findings suggest that cultivar selection, genetic manipulation, and environmental control could be important to obtain highly nutritious biomass in a CELSS.

NASA Discipline Number 61-20↗

In-Space Radiator Shape Optimization using Genetic Algorithms

Future space exploration missions will require the development of more advanced in-space radiators. These radiators should be highly efficient and lightweight, deployable heat rejection systems. Typical radiators for in-space heat mitigation commonly comprise a substantial portion of the total vehicle mass. A small mass savings of even 5-10% can greatly improve vehicle performance. The objective of this paper is to present the development of detailed tools for the analysis and design of in-space radiators using evolutionary computation techniques. The optimality criterion is defined as a two-dimensional radiator with a shape demonstrating the smallest mass for the greatest overall heat transfer, thus the end result is a set of highly functional radiator designs. This cross-disciplinary work combines topology optimization and thermal analysis design by means of a genetic algorithm The proposed design tool consists of the following steps; design parameterization based on the exterior boundary of the radiator, objective function definition (mass minimization and heat loss maximization), objective function evaluation via finite element analysis (thermal radiation analysis) and optimization based on evolutionary algorithms. The radiator design problem is defined as follows: the input force is a driving temperature and the output reaction is heat loss. Appropriate modeling of the space environment is added to capture its effect on the radiator. The design parameters chosen for this radiator shape optimization problem fall into two classes, variable height along the width of the radiator and a spline curve defining the -material boundary of the radiator. The implementation of multiple design parameter schemes allows the user to have more confidence in the radiator optimization tool upon demonstration of convergence between the two design parameter schemes. This tool easily allows the user to manipulate the driving temperature regions thus permitting detailed design of in-space radiators for unique situations. Preliminary results indicate an optimized shape following that of the temperature distribution regions in the "cooler" portions of the radiator. The results closely follow the expected radiator shape.

Hull, Patrick V.↗

Relationships Between HED's, Mesosiderites, and Ungrouped Achondrites: Trace Element Analyses of Mesosiderite RKPA 79015 and Ungrouped Achondrite QUE 93148

Achondritic meteorites are a diverse group of meteorites that formed by igneous activity in asteroids. These meteorites can provide important information about early differentiation processes on asteroidal bodies. The howardite-eucrite-diogenite (HED) meteorites, the largest group of achondrites, are the only group of meteorites for which a potential parent body has been identified (4 Vesta) [e.g., 1]. Mesosiderites are stony-iron meteorites composed of roughly equal amounts of metal and silicates and silicates are broadly similar to HED meteorites [2]. They may have been formed by impact-mixing of crustal and core materials of differentiated meteorite parent bodies. Chemical and oxygen isotopic compositional data suggest that the HED meteorites and silicate portions of mesosiderites originated on the same or closely related parent bodies. Pallasites and IIIAB irons also have similar oxygen isotope compositions and have been thought to be related to the HEDs [3,4]. However, recent high resolution analyses have shown that pallasites and HED's have different oxygen isotopic values, but mesosiderites and HED s have the same isotope compositions implying a close connection [5]. QUE 93148 is a small (1.1g) olivine-rich (mg 86) achondrite that contains variable amounts of orthopyroxenene (mg 87) and kamacite (6.7 wt% Ni), with minor augite [6]. This meteorite was originally classified as a lodranite [7], but it s oxygen isotopic composition precludes a genetic relationship to the acapulcoites and lodranites. And also this meteorite has a lower Mn/Mg ratio than any major group of primitive or evolved achondrites and suggested that QUE 93148 may be a piece of the deep mantle of the HED parent body [6]. To better understand the relationship between HED s, mesosiderites and related achondrites, we have measured trace elements in the individual metallic and silicate phases. In this study, abundances of a suite of elements were measured for the unusual mesosiderite RKPA 79015 and a ungrouped achondrite QUE93148.

Righter, M.↗

Multivariate Methods for Prediction of Geologic Sample Composition with Laser-Induced Breakdown Spectroscopy

Laser-induced breakdown spectroscopy (LIBS) uses pulses of laser light to ablate a material from the surface of a sample and produce an expanding plasma. The optical emission from the plasma produces a spectrum which can be used to classify target materials and estimate their composition. The ChemCam instrument on the Mars Science Laboratory (MSL) mission will use LIBS to rapidly analyze targets remotely, allowing more resource- and time-intensive in-situ analyses to be reserved for targets of particular interest. ChemCam will also be used to analyze samples that are not reachable by the rover's in-situ instruments. Due to these tactical and scientific roles, it is important that ChemCam-derived sample compositions are as accurate as possible. We have compared the results of partial least squares (PLS), multilayer perceptron (MLP) artificial neural networks (ANNs), and cascade correlation (CC) ANNs to determine which technique yields better estimates of quantitative element abundances in rock and mineral samples. The number of hidden nodes in the MLP ANNs was optimized using a genetic algorithm. The influence of two data preprocessing techniques were also investigated: genetic algorithm feature selection and averaging the spectra for each training sample prior to training the PLS and ANN algorithms. We used a ChemCam-like laboratory stand-off LIBS system to collect spectra of 30 pressed powder geostandards and a diverse suite of 196 geologic slab samples of known bulk composition. We tested the performance of PLS and ANNs on a subset of these samples, choosing to focus on silicate rocks and minerals with a loss on ignition of less than 2 percent. This resulted in a set of 22 pressed powder geostandards and 80 geologic samples. Four of the geostandards were used as a validation set and 18 were used as the training set for the algorithms. We found that PLS typically resulted in the lowest average absolute error in its predictions, but that the optimized MLP ANN and the CC ANN often gave results comparable to PLS. Averaging the spectra for each training sample and/or using feature selection to choose a small subset of wavelengths to use for predictions gave mixed results, with degraded performance in some cases and similar or slightly improved performance in other cases. However, training time was significantly reduced for both PLS and ANN methods by implementing feature selection, making this a potentially appealing method for initial, rapid-turn-around analyses necessary for Chemcam's tactical role on MSL. Choice of training samples has a strong influence on the accuracy of predictions. We are currently investigating the use of clustering algorithms (e.g. k-means, neural gas, etc.) to identify training sets that are spectrally similar to the unknown samples that are being predicted, and therefore result in improved predictions

Morris, Richard↗

Radiation Effect on Human Tissue

Predicting the occurrence of human cancer following exposure of an epidemiologic population to any agent causing genetic damage is a difficult task. To an approximation, this is because the uncertainty of uniform exposure to the damaging agent, and the uncertainty of uniform processing of that damage within a complex set of biological variables, degrade the confidence of predicting the delayed expression of cancer as a relatively rare event within clinically normal individuals. This situation begs the need for alternate controlled experimental models that are predictive for the development of human cancer following exposures to agents causing genetic damage. Such models historically have not been of substantial proven value. It is more recently encouraging, however, that developments in molecular and cell biology have led to an expanded knowledge of human carcinogenesis, and of molecular markers associated with that process. It is therefore appropriate to consider new laboratory models developed to accomodate that expanded knowledge in order to assess the cancer risks associated with exposures to genotoxic agents. When ionizing radiation of space is the genotoxic agent, then a series of additional considerations for human cancer risk assessment must also be applied. These include the dose of radiation absorbed by tissue at different locations in the body, the quality of the absorbed radiation, the rate at which absorbed dose accumulates in tissue, the way in which absorbed dose is measured and calculated, and the alterations in incident radiation caused by shielding materials. It is clear that human cancer risk assessment for damage caused by ionizing radiation is a multidisciplinary responsibility, and that within this responsibility no single discipline can hold disproportionate sway if a risk assessment model of radiation-induced human cancer is to be developed that has proven value. Biomolecular and cellular markers from the work reported here are considered for use in assessing human cancer risk related to exposure to space radiation. This potential use must be integrated within the specified multidisciplinary context in order to create a new tool of molecular epidemiology that can hopefully then realistically assess this cancer risk.

Richmond, Robert C.↗

Mars Exploration Rover APXS Results from Matijevic Hill

Correlation analysis of APXS results on the eastern slope rocks indicate that the Matijevic Hill rocks are overall compositionally distinct from the Shoemaker Formation rocks [6]. Compared to the Shoemaker impactites, Matijevic Hill rocks are higher in Al, Si, and Ni, and lower in Ti, Fe, and Zn. No significant variation is evident in the APXS analyses that indicate the presence of a smectite or other phyllosilicate, as opposed to basaltic rocks. However, APXS data cannot in themselves rule out phyllosilicates. If indeed this material contains smectite, as seen from orbit, it implies that the rock has been isochemically altered to create the phyllosilicate content. The Cl content of the Cape York rocks is relatively high, and whereas the S/Cl ratio in the Burns Formation is 4x higher than in soil, in the Cape York rocks it is lower than in soil. These trends indicate that the alteration processes and types of aqueous salt loads were different between Cape York and Meridiani. In addition, significant deviations from the Martian Mn/Fe ratio are observed in Whitewater Lake coatings and the altered Grasford/Deadwood rocks (Fig. 3). These variations indicate that the redox/pH conditions during alteration of the Shoemaker Formation rocks and the Matijevic Hill rocks were similar, but that the Deadwood/Grasberg unit may have undergone alteration under different conditions, possibly at a later time. The Matijevic Hill outcrops appear to share a common genetic origin. It is not yet clear whether both the Shoemaker impactites and Matijevic Hill rocks are related to the formation of Endeavour Crater, or whether the Matijevic Hill suite represents a prior episode of Martian impact or volcanism. Opportunity continues to investigate both hypotheses.

Cohen, B. A.↗

GL4U: Training the next generation of bioinformaticians, one omics datatype at a time

Spaceflight modifies gene expression in every organism examined to date, including humans. Understanding how these gene expression changes affect physiology is crucial for the development of countermeasures to enable long-duration manned missions. NASA’s GeneLab project provides researchers open access to multi-omics data, including genetic and gene expression data, from spaceflight experiments that can be mined to understand the effects of spaceflight on biological systems. To ensure new knowledge generation through data re-use, it is important to maximize the number of scientists who utilize GeneLab data. Training students on the GeneLab platform is the best way to create long-term adopters of this NASA database and its tools. Turning students into future instructors and advocates will also accelerate the dissemination of these data and tools to the broader scientific community. Therefore, in collaboration with the GeneLab Educational Working Group (EWG), GeneLab has created GeneLab for Colleges and Universities (GL4U). GL4U provides space biology-relevant training in bioinformatics to the next generation of scientists through direct and indirect approaches. The GeneLab team plans to host two annual data processing bootcamps, one for college-level students (direct) and one for college educators (indirect – training of trainers), in which participants learn to analyze GeneLab’s space-relevant omics data. During the bootcamp, educators will receive materials and training to enable them to run the bootcamp at their home institutions or alternatively to adapt the content to implement within existing courses, thereby extending the reach of this initiative. The GL4U direct training pilot program was conducted in June 2021 in collaboration with USRA and San Jose State University (SJSU). During the pilot, SJSU students participated in a week-long bootcamp consisting of space biology-specific lectures and hands-on instruction using Jupyter Notebooks to analyze RNA sequence data. This pilot demonstrates the capacity of GL4U for training young scientists and encouraging data re-use.

Jonathan Matthew Galazka↗

Volatile/mobile trace elements in meteoritic, non-lunar basalts: Guides to Martian sample contents

A variety of genetic processes on or in extraterrestrial objects can be examined by study of volatile/mobile trace elements. Doubtless, considerable efforts will be expended on determining these elements in returned Martian samples. The purpose is to estimate levels of such elements expected to be present in returned Martian samples. Some ideas about Martian genesis were already advanced from the volatile/mobile element contents in SNC meteorites, assuming that Mars was their parent body. Even is Mars and the SNC meteorite parent body are identical, compositional ranges for returned Martian samples should exceed those of SNC meteorites. It is expected, therefore, that Martian samples returned from locations other than Polar regions will have indigenous volatile/mobile element contents within howardite-diogenite ranges. Elements with strong lithophile tendences may be more abundant, as they are in many lunar samples. Most of these elements should be at ppb levels except for Co, Ga, Zn, and Rb, which should lie at ppm levels. If Martian volcanism was accompanied by fumarolic emanations, it should be reflected in occasional huge enrichments of mobile trance elements, as in lunar meteorite Y 791197. During collection and transport Earthward, samples must be contained under conditions appropriate to ppb concentrations. Materials must be used that will not cause contamination which occurred during the Apollo program, where indium from seals contaminated many samples.

Lipschutz, M. E.↗

PowerCell aboard the EuCROPIS satellite - Results from the first synthetic biology experiment in space

Synthetic biology is the design and construction of new biological parts and systems not found in nature, a technology that we believe will be a game-changing technology for space exploration. The PowerCell concept is an ecology based on photosynthetic microbes taking advantage of in situ materials and energy to generate, on-demand, useful products (food, fuel, cloths, drugs, etc.) that satisfy specific needs of long-term human presence away from Earth. This first PowerCell experiment is testing this concept as well as several key mechanisms of engineering biology in a 48-well plate microfluidics system. NASA's PowerCell experiment was loaded in the fluidics system at NASA Ames in May 2016, and subsequently integrated on the DLR's compact satellite Eu:CROPIS (Euglena Combined Regenerative Organic food Production In Space), as a secondary payload. Launched into Earth’s orbit from the Vandenberg Air Force Base, California, USA, in December 3, 2019, the Eu:CROPIS platform has provided an artificial gravity through rotational acceleration, simulating gravity ranges compatible to those found on Mars and on the Moon, as well as microgravity in the absence of acceleration. Each set of biological experiments performed at Earth's orbit was compared to ground controls. Over 400 days' worth of mission data showed that all hardware and software worked as planned, and microbial growth was observed in all four gravity regimes, including growth on cyanobacterial cell extract in three out of four gravity regimes. The genetic transformation experiment was successful in microgravity and provided valuable long-term reagent stability data, including antibiotic efficacy. The demonstrated ability of hardy bacterial spores to recover from a long-term stasis period will be fundamental for deep human space missions. Lessons learned from hardware design to experimental operations are leading to improved designs and capabilities for future missions.

Ivan Glaucio Paulino Lima↗

Structural Maps of the V-17 Beta Regio Quadrangle, Venus

These represent slices of the geologic map into 7 time-stratigraphic levels whose descriptions are found in [3-6]. From older to younger they are: 1) Tessera material unit (t), 2) Densely fractured plains material unit (pdf), 3) Fractured and ridged plains material unit (pfr), 4) Tessera transitional terrain structural unit (tt), 5) Fracture belts structural unit (fb), 6) Shield plains (psh) and plains with wrinkle ridges (pwr) material units combined, and 7) Lobate (pl) and smooth (ps) plains material units combined and, approximately contemporaneous with them, the structural unit of rifted terrain (rt). Each slice shows the generalized pattern of structures typical of these units. Figures 1-7 show the seven maps and Figure 8 shows the combined map illustrating what is shown in the seven maps. To visualize the Beta Regio uplift outlines, the major structure of this area, we show the +0.5 km and +2.5 km contour lines, corresponding respectively to the base and the mid-height of the uplift. It is seen in Figures 1-2 and 4 the trends of t, pdf and tt occupy relatively small areas and their structures seen in these small windows appear rather variable and with almost no orientation heritage with time. Figure 3 shows that swarms of ridge belts trend mostly NW and go through the Beta structure with no alignment with it, suggesting that this structure did not yet exist at this time. Figure 5 shows that fracture belts align along the northern base of the Beta uplift suggesting onset of the formation of this structure. Figure 6 shows that wrinkle ridges do not show alignment with the Beta uplift suggesting that this already forming structure was not high enough to exert topographic stress in its vicinity. Figure 7 shows that the Beta uplift has Devana Chasma as an axial rift zone, suggesting a genetic link between the uplift and rifting. Figure 8 shows that structural trends in this area significantly changed with time.

Basilevsky, A. t.↗

A Quantitative Study of Oxygen as a Metabolic Regulator

An acute reduction in oxygen (O2) delivery to a tissue is generally associated with a decrease in phosphocreatine, increases in ADP, NADH/NAD, and inorganic phosphate, increased rates of glycolysis and lactate production, and reduced rates of pyruvate and fatty acid oxidation. However, given the complexity of the human bioenergetic system and its components, it is difficult to determine quantitatively how cellular metabolic processes interact to maintain ATP homeostasis during stress (e.g., hypoxia, ischemia, and exercise). Of special interest is the determination of mechanisms relating tissue oxygenation to observed metabolic responses at the tissue, organ, and whole body levels and the quantification of how changes in tissue O2 availability affect the pathways of ATP synthesis and the metabolites that control these pathways. In this study, we extend a previously developed mathematical model of human bioenergetics to provide a physicochemical framework that permits quantitative understanding of O2 as a metabolic regulator. Specifically, the enhancement permits studying the effects of variations in tissue oxygenation and in parameters controlling the rate of cellular respiration on glycolysis, lactate production, and pyruvate oxidation. The whole body is described as a bioenergetic system consisting of metabolically distinct tissue/organ subsystems that exchange materials with the blood. In order to study the dynamic response of each subsystem to stimuli, we solve the ordinary differential equations describing the temporal evolution of metabolite levels, given the initial concentrations. The solver used in the present study is the packaged code LSODE, as implemented in the NASA Lewis kinetics and sensitivity analysis code, LSENS. A major advantage of LSENS is the efficient procedures supporting systematic sensitivity analysis, which provides the basic methods for studying parameter sensitivities (i.e., changes in model behavior due to parameter variation). Sensitivity analysis establishes relationships between model predictions and problem parameters (i.e., initial concentrations, rate coefficients, etc). It helps determine the effects of uncertainties or changes in these input parameters on the predictions, which ultimately are compared with experimental observations in order to validate the model. Sensitivity analysis can identify parameters that must be determined accurately because of their large effect on the model predictions and parameters that need not be known with great precision because they have little or no effect on the solution. This capability may prove to be important in optimizing the design of experiments, thereby reducing the use of animals. This approach can be applied to study the metabolic effects of reduced oxygen delivery to cardiac muscle due to local myocardial ischemia and the effects of acute hypoxia on brain metabolism. Other important applications of sensitivity analysis include identification of quantitatively relevant pathways and biochemical species within an overall mechanism, when examining the effects of a genetic anomaly or pathological state on energetic system components and whole system behavior.

Radhakrishnan, Krishnan↗

Probing Neodymium Isotopic Variations in the Inner Solar System

One of the key problems in planetary science is the identification of the building blocks of Earth, and whether they exist within our current collection of meteorites. Stable mass independent isotopic anomalies, usually nucleosynthetic in origin, are a key tool in fingerprinting material coming from different accretionary regions within the protoplanetary disk. For a number of isotopic systems such as O, Ni, Ti and Cr, enstatite chondrites (EC) appear to be the strongest candidates for Earth's building blocks, despite their low Mg/Si ratios relative to the bulk Earth. It has been proposed that Earth, the Moon-forming impactor and enstatite chondrites all were originally sourced from the same reservoir in the protoplanetary disk, but subsequently experienced divergent chemical evolution pathways. This was recently challenged by who used the correlation between Mo and Nd isotopes in bulk meteorites to argue that such a reservoir does not exist as the isotopic composition of enstatite chondrites is resolvable from Earth. However, in detail the Nd isotopic ratios of EC ((142)Nd/(144)Nd, (148)Nd/(144)Nd, (150)Nd/(144)Nd) show considerable variability, and overlap significantly with the isotopic composition of both the bulk Earth and ordinary chondrites (OC). previously identified Nd isotopic variability in EC, linking it to the degree of equilibration the meteorite had experienced, however they only focused on collecting high precision (142)Nd/(144)Nd, which also has contributions from the decay of the short-lived Sm-146 nuclide (t(sub 1/2) ~103 Myr), making identification of nucleosynthetic anomalies less clear. While the study of reports all the Nd isotopic ratios measured at high precision, only equilibrated EC were analyzed. Therefore, with the currently published data it is unclear to what extent thermal equilibration in the EC is responsible for the variation observed in the stable Nd isotopic ratios, and whether the EC reservoir can be resolved from Earth. In order to better understand the genetic relationship between enstatite chondrites and the Earth we are carrying out a more systematic study including both equilibrated and unequilibrated enstatite chondrites, focusing on high precision analysis of all the stable Nd isotopic ratios.

SOLAR SYSTEM↗

The effect of track structure on the induction of chromosomal aberrations in murine cells

PURPOSE: To measure chromosome aberrations in C3H 10T1/2 mouse fibroblasts using FISH painting at the first mitosis following exposure to 30 keV/microm hydrogen or neon ions. MATERIALS AND METHODS: Cells in plateau-phase were irradiated with 0.86 MeV protons at the TTT-3 Tandem accelerator in Naples (Italy), or with 400 MeV/n Ne ions at the HIMAC accelerator in Chiba (Japan). Colcemid-blocked cells were harvested at the first mitosis following exposure, and chromosome spreads were hybridized in situ with a fluorescein-labelled composite mouse DNA probe specific for chromosomes 2 and 8. RESULTS: Protons were more efficient than neon ions at the same LET in the induction of chromosome interchanges and breaks. Yields of complex exchanges were similar for both particles at the same dose, but protons produced mostly insertions, while with Ne exposure non-reciprocal exchanges were the most frequent complex-type exchange. CONCLUSIONS: Charged particles with the same LET produce different yields of chromosome aberrations, and some observed differences can be explained based on the available track-structure models.

NASA Center JSC↗