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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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At least 199 records · Page 11
All sourcesNASA NTRSDOE OSTI
NASA NTRS1990

Sequence of Tissue Responses in the Early Stages of Experimental Allergic Encephalomyelitis (EAE): Immunohistochemical, Light Microscopic, and Ultrastructural Observations in the Spinal Cord

Experimental allergic encephalomyelitis (EAE) was induced in adult Lewis rats with purified guinea pig CNS myelin and Freund's adjuvant. As soon as the very earliest clinical signs appeared the animals were perfused with fixatives and the spinal cord analyzed by electron microscopy, silver methods, and immunocytochemistry. Our findings suggest that in the early stages of EAE a sequence of events can be traced, although these events frequently overlap. The earliest morphological change appears to be astrocytic edema in both the cell body and processes. Increased amounts of glycogen particles and dispersion of glial filaments are prominent. These changes seem to occur just prior to the time when inflammatory cells begin to penetrate the capillary walls. Invasion of the neuropil mainly by macrophages and lymphocytes closely follows. Both macrophages and microglia seem to participate in phagocytosis of oligodendrocytes and myelin. Demyelination, however, is not a prominent feature at this early stage.

DAmelio, Fernando E.↗
NASA NTRS1997

Observational Search for Cometary Aging Processes

The scientific objectives of this study were (i) to search for physical differences in the behavior of the dynamically new comets (those which are entering the solar system for the first time from the Oort cloud) and the periodic comets, and (ii) to interpret these differences, if any, in terms of the physical and chemical nature of the comets and the evolutionary histories of the two comet groups. Because outer solar system comets may be direct remnants of the planetary formation processes, it is clear that the understanding of both the physical characteristics of these bodies at the edge of the planet forming zone and of their activity at large heliocentric distances, r, will ultimately provide constraints on the planetary formation process both in our Solar System and in extra-solar planetary systems. A combination of new solar system models which suggest that the protoplanetary disk was relatively massive and as a consequence comets could form at large distances from the sun (e.g. from the Uranus-Neptune region to the vicinity of the Kuiper belt), observations of activity in comets at large r, and laboratory experiments on low temperature volatile condensation, are dramatically changing our understanding of the chemical'and physical conditions in the early solar nebula. In order to understand the physical processes driving the apparent large r activity, and to address the question of possible physical and chemical differences between periodic, non-periodic and Oort comets, the PI has been undertaking a long-term study of the behavior of a significant sample of these comets (approximately 50) over a wide range of r to watch the development, disappearance and changing morphology of the dust coma. The ultimate goal is to search for systematic physical differences between the comet classes by modelling the coma growth in terms of volatile-driven activity. The systematic observations for this have been ongoing since 1986, and have been obtained over the course of approximately 300 nights using the telescopes on Mauna Kea, Kitt Peak, Cerro Tololo, the European Southern Observatory, and several other large aperture facilities. A greater than 2 TB database of broad band comet images has been obtained which follows the systematic development and fading of the cometary coma for the comets in the database. The results to date, indicate that there is a substantial difference in the brightness and the amount of dust as a function of r between the two comet classes. In addition to this major finding, the program has been responsible for several exciting discoveries, including: the P/Halley outburst at r = 14.3 AU, the discovery of Chiron's coma and modelling and observations of the gravitationally bound component, observational evidence that activity continues out beyond r = 17 AU for many dynamically new comets

Meech, Karen J.↗
NASA NTRS2002

A Solar System Perspective on Laboratory Astrophysics

Planetary science deals with a wide variety of natural materials in a wide variety of environments. These materials include metals, minerals, ices, gases, plasmas, and organic chemicals. In addition, the newly defined discipline of astrobiology introduces biological materials to planetary science. The environments range from the interiors of planets with megapascal pressures to planetary magnetospheres, encompassing planetary mantles, surfaces, atmospheres, and ionospheres. The interplanetary environment includes magnetic and electrical fields, plasma, and dust. In order to understand planetary processes over these vast ranges, the properties of materials must be known, and most of the necessary information comes from the laboratory. Observations of the bodies and materials in the Solar System are accomplished over the full range of the electromagnetic spectrum by remote sensing from Earth or spacecraft. Comets exemplify this; molecular and atomic identifications are made from the hard ultraviolet to radio wavelengths, while X-rays are emitted as comets interact with the solar wind. Gamma rays from the surfaces of the Moon and asteroids are diagnostic of the mineral and ice content of those bodies; eventually, gamma rays will also be observed by probes to comets. A number of planetary materials are available in the laboratory for extensive Study: rocks from the Moon, Mars, several asteroids, as well as dust from comets (and perhaps the Kuiper Belt) are closely studied at every level, including atomic (isotopic). Even pre-solar interstellar grains isolated from meteorites are scrutinized for composition and crystalline structure. Beyond the materials themselves, various agents and processes have altered them over the 4.6-Gy age of the Solar System. Solar radiation, solar wind particles, trapped magnetospheric particles, cosmic rays, and micrometeoroid impacts have produced chemical, physical, and morphological changes in the atmospheres and on the surfaces of all planetary bodies. These processes are not well understood, so studies in a laboratory setting are especially needed.

Cruikshank, Dale P.↗
NASA NTRS1998

Gravitropism and development of wild-type and starch-deficient mutants of Arabidopsis during spaceflight

The "starch-statolith" hypothesis has been used by plant physiologists to explain the gravity perception mechanism in higher plants. In order to help resolve some of the controversy associated with ground-based research that has supported this theory, we performed a spaceflight experiment during the January 1997 mission of the Space Shuttle STS-81. Seedlings of wild-type (WT) Arabidopsis, two reduced-starch strains, and a starchless mutant were grown in microgravity and then given a gravity stimulus on a centrifuge. In terms of development in space, germination was greater than 90% for seeds in microgravity, and flight seedlings were smaller (60% in total length) compared to control plants grown on the ground and to control plants on a rotating clinostat. Seedlings grown in space had two structural features that distinguished them from the controls: a greater density of root hairs and an anomalous hypocotyl hook structure. However, the slower growth and morphological changes observed in the flight seedlings may be due to the effects of ethylene present in the spacecraft. Nevertheless, during the flight hypocotyls of WT seedlings responded to a unilateral 60 min stimulus provided by a 1-g centrifuge while those of the starch-deficient strains did not. Thus the strain with the greatest amount of starch responded to the stimulus given in flight and therefore, these data support the starch-statolith model for gravity sensing.

short duration↗
NASA NTRS2003

Exposure to Carbon Nanotube Material: Assessment of Nanotube Cytotoxicity Using Human Keratinocyte Cells

Carbon nanotubes are new members of carbon allotropes similar to fullerenes and graphite. Because of their unique electrical, mechanical, and thermal properties, carbon nanotubes are important for novel applications in the electronics, aerospace, and computer industries. Exposure to graphite and carbon materials has been associated with increased incidence of skin diseases, such as carbon fiber dermatitis, hyperkeratosis, and naevi. We investigated adverse effects of single-wall carbon nanotubes (SWCNT) using a cell culture of immortalized human epidermal keratinocytes (HaCaT). After 18 h of exposure of HaCaT to SWCNT, oxidative stress and cellular toxicity were indicated by formation of free radicals, accumulation of peroxidative products, antioxidant depletion, and loss of cell viability. Exposure to SWCNT also resulted in ultrastructural and morphological changes in cultured skin cells. These data indicate that dermal exposure to unrefined SWCNT may lead to dermal toxicity due to accelerated oxidative stress in the skin of exposed workers.

Keratinocytes/drug effects↗
NASA NTRS2001

Induced compression wood formation in Douglas fir (Pseudotsuga menziesii) in microgravity

In the microgravity environment of the Space Shuttle Columbia (Life and Microgravity Mission STS-78), were grown 1-year-old Douglas fir and loblolly pine plants in a NASA plant growth facility. Several plants were harnessed (at 45 degrees ) to establish if compression wood biosynthesis, involving altered cellulose and lignin deposition and cell wall structure would occur under those conditions of induced mechanical stress. Selected plants were harnessed at day 2 in orbit, with stem sections of specific plants harvested and fixed for subsequent microscopic analyses on days 8, 10 and 15. At the end of the total space mission period (17 days), the remaining healthy harnessed plants and their vertical (upright) controls were harvested and fixed on earth. All harnessed (at 45 degrees ) plant specimens, whether grown at 1 g or in microgravity, formed compression wood. Moreover, not only the cambial cells but also the developing tracheid cells underwent significant morphological changes. This indicated that the developing tracheids from the primary cell wall expansion stage to the fully lignified maturation stage are involved in the perception and transduction of the stimuli stipulating the need for alteration of cell wall architecture. It is thus apparent that, even in a microgravity environment, woody plants can make appropriate corrections to compensate for stress gradients introduced by mechanical bending, thereby enabling compression wood to be formed. The evolutionary implications of these findings are discussed in terms of "variability" in cell wall biosynthesis.

manned↗
NASA NTRS1999

Extracellular signal-regulated kinases 1 and 2 activation in endothelial cells exposed to cyclic strain

The aim of this study was to determine whether extracellular signal-regulated kinases 1/2 (ERK1/ERK2) are activated and might play a role in enhanced proliferation and morphological change induced by strain. Bovine aortic endothelial cells (BAEC) were subjected to an average of 6 or 10% strain at a rate of 60 cycles/min for up to 4 h. Cyclic strain caused strain- and time-dependent phosphorylation and activation of ERK1/ERK2. Peak phosphorylation and activation of ERK1/ERK2 induced by 10% strain were at 10 min. A specific ERK1/ERK2 kinase inhibitor, PD-98059, inhibited phosphorylation and activation of ERK1/ERK2 but did not inhibit the increased cell proliferation and cell alignment induced by strain. Treatment of BAEC with 2,5-di-tert-butyl-1, 4-benzohydroquinone, to deplete inositol trisphosphate-sensitive calcium storage, and gadolinium chloride, a Ca2+ channel blocker, did not inhibit the activation of ERK1/ERK2. Strain-induced ERK1/ERK2 activation was partly inhibited by the protein kinase C inhibitor calphostin C and completely inhibited by the tyrosine kinase inhibitor genistein. These data suggest that 1) ERK1/ERK2 are not critically involved in the strain-induced cell proliferation and orientation, 2) strain-dependent activation of ERK1/ERK2 is independent of intracellular and extracellular calcium mobilization, and 3) protein kinase C activation and tyrosine kinase regulate strain-induced activation of ERK1/ERK2.

NASA Discipline Cell Biology↗
NASA NTRS1998

Ensembles of radial basis function networks for spectroscopic detection of cervical precancer

The mortality related to cervical cancer can be substantially reduced through early detection and treatment. However, current detection techniques, such as Pap smear and colposcopy, fail to achieve a concurrently high sensitivity and specificity. In vivo fluorescence spectroscopy is a technique which quickly, noninvasively and quantitatively probes the biochemical and morphological changes that occur in precancerous tissue. A multivariate statistical algorithm was used to extract clinically useful information from tissue spectra acquired from 361 cervical sites from 95 patients at 337-, 380-, and 460-nm excitation wavelengths. The multivariate statistical analysis was also employed to reduce the number of fluorescence excitation-emission wavelength pairs required to discriminate healthy tissue samples from precancerous tissue samples. The use of connectionist methods such as multilayered perceptrons, radial basis function (RBF) networks, and ensembles of such networks was investigated. RBF ensemble algorithms based on fluorescence spectra potentially provide automated and near real-time implementation of precancer detection in the hands of nonexperts. The results are more reliable, direct, and accurate than those achieved by either human experts or multivariate statistical algorithms.

Cervix Neoplasms/pathology↗
NASA NTRS1996

The effect of nonablative laser energy on joint capsular properties. An in vitro histologic and biochemical study using a rabbit model

The purpose of this study was to evaluate the effect of laser energy at nonablative levels on joint capsular histologic and biochemical properties in an in vitro rabbit model. The medial and lateral portions of the femoropatellar joint capsule from both stifles of 12 mature New Zealand White rabbits were used. Specimens were divided into three treatment groups (5 watts, 10 watts, and 15 watt) and one control group using a randomized block design. Specimens were placed in a 37 degrees bath of lactated Ringer's solution and laser energy was applied using a holmium:yttrium-aluminum-garnet laser in four transverse passes across the tissue at a velocity of 2 mm/sec with the handpiece set 1.5 mm from the synovial surface. Histologic analysis revealed thermal alteration of collagen (fusion) and fibroblasts (pyknosis) at all energy densities, with higher laser energy causing significantly greater morphologic changes over a larger area (P < 0.05). Application of laser energy did not significantly alter the biochemical parameters evaluated, including type I collagen content and nonreducible crosslinks (P > 0.05). This study demonstrated that nonablative laser energy caused significant thermal damage to the joint capsular tissue in an energy-dependent fashion, but type I collagen content and nonreducible crosslinks (P > 0.05). This study demonstrated that nonablative laser energy caused significant thermal damage to the joint capsular tissue in an energy-dependent fashion, but type I Collagen content and nonreducible corsslinks were not significantly altered.

NASA Discipline Musculoskeletal↗
NASA NTRS2005

Tectonic-Climate Interactions in Action Orogenic Belts: Quantification of Dynamic Topography with SRTM data

This project was undertaken to examine the approach to steady state in collisional mountain belts. Although the primary thrust of this grant was to look at larger collisional mountain belts, such as the Himalaya, the Tien Shan, and Southern Alps, we began by looking at smaller structures represented by growing and propagating folds. Like ranges that are evolving toward a topographic steady state, these folds undergo a series of morphologic changes as they are progressively uplifted and eroded. We wanted to document the nature of these changes and to try to discern some of the underlying controls on them. We initially focused on the Wheeler Ridge anticline in southern California. Subsequently, we progressed to looking at the topographic development and the effects of differential uplift and glaciation on the Kyrgyz Range in the northern Tien Shan. This range is unusual inasmuch as it is transformed along its length from a simple uplift with a largely preserved Mesozoic erosion surface arching across it to a highly dissected and heavily glaciated uplift in the region where uplift has been sustained at higher rates over longer intervals. In efforts to understand the distribution of erosion rates at 10(exp 3) - 10(exp 5) year time scales, cosmogenic radionuclide (CRN) concentrations have been gaining increasingly widespread usage (Brown et al., 1995; Riebe et al., 2004; Riebe et al., 2001; Vance et al., 2003). Most studies to date, however, have been conducted in slowly eroding ranges. In rapidly eroding mountains where landslides deliver most of the sediments to the rivers, we hypothesized that CRN concentrations could be highly perturbed by the stochastic processes of landsliding. Therefore, we undertook the development of a numerical model that simulated the effects of both landsliding and grain-by-grain attrition within fluvial catchments. This modeling effort has shown the effects of catchment size and erosion rate on CRN concentrations and allows a prediction of where to sample to obtain the optimal erosion rate estimates using CRN techniques. Finally, we developed computational techniques to operate on DEMs to extract useful information that would enable quantification of climate-erosion interactions. In particular, we worked on rapid techniques to define catchments of any given range of sizes, to extract channel gradients, to combine precipitation information to calculate discharge, and to utilize various stream-power models to determine the erosional energy within any given catchment within a transect. We briefly describe results from Wheeler Ridge, the Kyrgyz Range, the Nepal Himalaya, and our numerical modeling.

Burbank, Douglas W.↗
NASA NTRS2005

Protection of Conductive and Non-conductive Advanced Polymer-based Paints from Highly Aggressive Oxidative Environments

Research has been continued to further improve the space durability of conductive and non-conductive polymer-based paints and of conductive thermal control paints for space applications. Efforts have been made to enhance the space durability and stability of functional Characteristics in ground-based space environment imitating conditions, using specially developed surface modification treatment. The results of surface modification of new conductive paints, including the ground-based testing in aggressive oxidative environments, such as atomic oxygen/UV and oxygen plasma, and performance evaluation are presented. Functional properties and performance characteristics, such as thermal optical properties (differential solar absorptance and thermal emittance representing the thermal optical performance of thermal control paints) and surface resistivity characteristics of pristine, surface modified, and tested materials were verified. Extensive surface analysis studies have been performed using complementary surface analyses including SEM/EDS and XPS. Test results revealed that the successfully treated materials exhibit reduced mass loss and no surface morphology change, thus indicating good protection from the severe oxidative environment. It was demonstrated that the developed surface modification treatment could be applied successfully to charge dissipative and conductive paints.

Gudimenko, Y.↗
NASA NTRS1999

New VLA Observations for the HH 1-2 Region: Evidence for Density Enhancements Moving Along the Axis of the VLA 1 Radio Jet

Using the Very Large Array, we have carried out new, sensitive radio continuum observations at 6 and 3.6 cm of the HH 1-2 region. The comparison between the 6 cm maps made from data taken in 1986.2 and 1992.9 indicates that VLA 1, the exciting source of the HH 1-2 flow, has suffered a morphological change that is attributed to the motion of a symmetric pair of knots along the axis of the radio jet.

jets and outflows stars pre-main-sequence↗
NASA NTRS2007

Spacecraft Sterilization Using Non-Equilibrium Atmospheric Pressure Plasma

As a solution to chemically and thermally destructive sterilization methods currently used for spacecraft, non-equilibrium atmospheric pressure plasmas are used to treat surfaces inoculated with Bacillus subtilis and Deinococcus radiodurans. Evidence of significant morphological changes and reduction in viability due to plasma exposure will be presented, including a 4-log reduction of B. subtilis after 2 minutes of dielectric barrier discharge treatment.

disinfection↗
NASA NTRS2008

Closing the Phenotypic Gap between Transformed Neuronal Cell Lines in Culture and Untransformed Neurons

Studies of neuronal dysfunction in the central nervous system (CNS) are frequently limited by the failure of primary neurons to propagate in vitro. Neuronal cell lines can be substituted for primary cells but they often misrepresent normal conditions. We hypothesized that a dimensional (3-D) cell culture system would drive the phenotype of transformed neurons closer to that of untransformed cells. In our studies comparing 3-D versus 2-dimensional (2-D) culture, neuronal SH-SY5Y (SY) cells underwent distinct morphological changes combined with a significant drop in their rate of cell division. Expression of the proto-oncogene N-myc and the RNA binding protein HuD was decreased in 3-D culture as compared to standard 2-D conditions. We observed a decline in the anti-apoptotic protein Bcl-2 in 3-D culture, coupled with increased expression of the pro-apoptotic proteins Bax and Bak. Moreover, thapsigargin (TG)-induced apoptosis was enhanced in the 3-D cells. Microarray analysis demonstrated significantly differing mRNA levels for over 700 genes in the cells of each culture type. These results indicate that a 3-D culture approach narrows the phenotypic gap between neuronal cell lines and primary neurons. The resulting cells may readily be used for in vitro research of neuronal pathogenesis.

Myers, Tereance A.↗
NASA NTRS2006

Assessment of Postflight Locomotor Performance Utilizing a Test of Functional Mobility: Strategic and Adaptive Responses

Space flight induces adaptive modification in sensorimotor function, allowing crewmembers to operate in the unique microgravity environment. This adaptive state, however, is inappropriate for a terrestrial environment. During a re-adaptation period upon their return to Earth, crewmembers experience alterations in sensorimotor function, causing various disturbances in perception, spatial orientation, posture, gait, and eye-head coordination. Following long duration space flight, sensorimotor dysfunction would prevent or extend the time required to make an emergency egress from the vehicle; compromising crew safety and mission objectives. We are investigating two types of motor learning that may interact with each other and influence a crewmember's ability to re-adapt to Earth's gravity environment. In strategic learning, crewmembers make rapid modifications in their motor control strategy emphasizing error reduction. This type of learning may be critical during the first minutes and hours after landing. In adaptive learning, long-term plastic transformations occur, involving morphological changes and synaptic modification. In recent literature these two behavioral components have been associated with separate brain structures that control the execution of motor strategies: the strategic component was linked to the posterior parietal cortex and the adaptive component was linked to the cerebellum (Pisella, et al. 2004). The goal of this paper was to demonstrate the relative contributions of the strategic and adaptive components to the re-adaptation process in locomotor control after long duration space flight missions on the International Space Station (ISS). The Functional Mobility Test (FMT) was developed to assess crewmember s ability to ambulate postflight from an operational and functional perspective. Sixteen crewmembers were tested preflight (3 sessions) and postflight (days 1, 2, 4, 7, 25) following a long duration space flight (approx 6 months) on the ISS. We have further analyzed the FMT data to characterize strategic and adaptive components during the postflight readaptation period. Crewmembers walked at a preferred pace through an obstacle course set up on a base of 10 cm thick medium density foam (Sunmate Foam, Dynamic Systems, Inc., Leicester, NC). The 6.0m X 4.0m course consisted of several pylons made of foam; a Styrofoam barrier 46.0cm high that crewmembers stepped over; and a portal constructed of two Styrofoam blocks, each 31cm high, with a horizontal bar covered by foam and suspended from the ceiling which was adjusted to the height of the crewmember s shoulder. The portal required crewmembers to bend at the waist and step over a barrier simultaneously. All obstacles were lightweight, soft and easily knocked over. Crewmembers were instructed to walk through the course as quickly and as safely as possible without touching any of the objects on the course. This task was performed three times in the clockwise direction and three times in the counterclockwise direction that was randomly chosen. The dependent measures for each trial were: time to complete the course (seconds) and the number of obstacles touched or knocked down. For each crewmember, the time to complete each FMT trial from postflight days 1, 2, 4, 7 and 25 were further analyzed. A single logarithmic curve using a least squares calculation was fit through these data to produce a single comprehensive curve (macro). This macro curve composed of data spanning 25 days, illustrates the re-adaptive learning function over the longer time scale term. Additionally, logarithmic curves were fit to the 6 data trials within each individual post flight test day to produce 5 separate daily curves. These micro curves, produced from data obtained over the course of minutes, illustrates the strategic learning function exhibited over a relative shorter time scale. The macro curve for all subjects exhibited adaptive motor learning patterns over the 25 day period. Howev, 9/16 crewmembers exhibited significant strategic motor learning patterns in their micro curves, as defined by m > 1 in the equation of the line y=m*LN(x) +b. These data indicate that postflight recovery in locomotor function involves both strategic and adaptive mechanisms. Future countermeasures will be designed to enhance both recovery processes.

Warren, L. E.↗
NASA NTRS2008

Innovative Applications of Laser Scanning and Rapid Prototype Printing to Rock Breakdown Experiments

We present the novel application of two technologies for use in rock breakdown experiments, i.e. close-range, ground-based 3D triangulation scanning and rapid prototype printing. These techniques aid analyses of form-process interactions across the range of scales relevant to breakdown (micron-m). This is achieved through (a) the creation of DEMs (which permit quantitative description and analysis of rock surface morphology and morphological change) and (b) the production of more realistically-shaped experimental blocks. We illustrate the use of these techniques, alongside appropriate data analysis routines, in experiments designed to investigate the persistence of fluvially-derived features in the face of subsequent wind abrasion and weathering. These techniques have a range of potential applications in experimental field and lab-based geomorphic studies beyond those specifically outlined here.

Bourke, Mary↗
NASA NTRS2011

Imaging Modalities Relevant to Intracranial Pressure Assessment in Astronauts

Learning Objectives of this slide presentation are: 1: To review the morphological changes in orbit structures caused by elevated Intracranial Pressure (ICP), and their imaging representation. 2: To learn about the similarities and differences between MRI and sonographic imaging of the eye and orbit. 3: To learn about the role of MRI and sonography in the noninvasive assessment of intracranial pressure in aerospace medicine, and the added benefits from their combined interpretation.

Sargsyan, Ashot E.↗
NASA NTRS2010

In-Situ TEM-STM Observations of SWCNT Ropes/Tubular Transformations

Single-walled carbon nanotubes (SWCNTs) prepared by the HiPco process were purified using a modified gas phase purification technique. A TEM-STM holder was used to study the morphological changes of SWCNT ropes as a function of applied voltage. Kink formation, buckling behavior, tubular transformation and eventual breakdown of the system were observed. The tubular formation was attributed to a transformation from SWCNT ropes to multi-walled carbon nanotube (MWCNT) structures. It is likely mediated by the patching and tearing mechanism which is promoted primarily by the mobile vacancies generated due to current-induced heating and, to some extent, by electron irradiation.

Sola, F.↗