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At least 217 records · Page 12

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NASA NTRS1998

Use of an adaptable cell culture kit for performing lymphocyte and monocyte cell cultures in microgravity

The results of experiments performed in recent years on board facilities such as the Space Shuttle/Spacelab have demonstrated that many cell systems, ranging from simple bacteria to mammalian cells, are sensitive to the microgravity environment, suggesting gravity affects fundamental cellular processes. However, performing well-controlled experiments aboard spacecraft offers unique challenges to the cell biologist. Although systems such as the European 'Biorack' provide generic experiment facilities including an incubator, on-board 1-g reference centrifuge, and contained area for manipulations, the experimenter must still establish a system for performing cell culture experiments that is compatible with the constraints of spaceflight. Two different cell culture kits developed by the French Space Agency, CNES, were recently used to perform a series of experiments during four flights of the 'Biorack' facility aboard the Space Shuttle. The first unit, Generic Cell Activation Kit 1 (GCAK-1), contains six separate culture units per cassette, each consisting of a culture chamber, activator chamber, filtration system (permitting separation of cells from supernatant in-flight), injection port, and supernatant collection chamber. The second unit (GCAK-2) also contains six separate culture units, including a culture, activator, and fixation chambers. Both hardware units permit relatively complex cell culture manipulations without extensive use of spacecraft resources (crew time, volume, mass, power), or the need for excessive safety measures. Possible operations include stimulation of cultures with activators, separation of cells from supernatant, fixation/lysis, manipulation of radiolabelled reagents, and medium exchange. Investigations performed aboard the Space Shuttle in six different experiments used Jurkat, purified T-cells or U937 cells, the results of which are reported separately. We report here the behaviour of Jurkat and U937 cells in the GCAK hardware in ground-based investigations simulating the conditions expected in the flight experiment. Several parameters including cell concentration, time between cell loading and activation, and storage temperature on cell survival were examined to characterise cell response and optimise the experiments to be flown aboard the Space Shuttle. Results indicate that the objectives of the experiments could be met with delays up to 5 days between cell loading into the hardware and initial in flight experiment activation, without the need for medium exchange. Experiment hardware of this kind, which is adaptable to a wide range of cell types and can be easily interfaced to different spacecraft facilities, offers the possibility for a wide range of experimenters successfully and easily to utilise future flight opportunities.

short duration↗

NASA NTRS1996

Exercise as a countermeasure for physiological adaptation to prolonged spaceflight

Exercise represents the primary countermeasure used during spaceflight to maintain or restore maximal aerobic capacity (VO2max), musculoskeletal structure, and orthostatic function. However, no single exercise or combination of prescriptions has proven entirely effective in restoring cardiovascular and musculoskeletal functions to preflight levels following prolonged spaceflight. As human spaceflight exposures increase in duration, assessment and development of various effective exercise-based protective procedures become paramount. This must involve improvement in specific countermeasure prescription as well as development of additional approaches that will allow space travelers greater flexibility and medical safety during long flights. Effective exercise prescription will be based on identification of basic physiological stimuli that maintain normal function in terrestrial gravity and understanding of how specific combinations of exercise characteristics e.g., duration, frequency, intensity, mode) can mimic these stimuli and affect the overall process of adaptation to microgravity. This can be accomplished only with greater emphasis of research on ground-based experiments. Future attention must be directed to improving exercise compliance while minimizing both crew time and the impact of the exercise on life-support resources.

Skylab Project↗

NASA NTRS2005

Issues that Drive Waste Management Technology Development for Space Missions

Waste management technologies for space life support systems are currently at low development levels. Manual compaction of waste in plastic bags and overboard disposal to earth return vehicles are the primary current waste management methods. Particularly on future missions, continuance of current waste management methods would tend to expose the crew to waste hazards, forfeit recoverable resources such as water, consume valuable crew time, contaminate planetary surfaces, and risk return to Earth of extraterrestrial life. Improvement of waste management capabilities is needed for adequate management of wastes. Improvements include recovery of water and other resources, conversion of waste to states harmless to humans, long-term containment of wastes, and disposal of waste. Current NASA requirements documents on waste management are generally not highly detailed. More detailed requirements are needed to guide the development of waste management technologies that will adequately manage waste. In addition to satisfying requirements, waste management technologies must also recover resources. Recovery of resources such as water and habitat volume can reduce mission cost. This paper explores the drivers for waste management technology development including requirements and resource recovery.

Fisher, John W.↗

NASA NTRS2000

Growth and Morphology of Supercritical Fluids Studied in Microgravity on Mir

The Growth and Morphology of Supercritical Fluids (GMSF) is an international experiment facilitated by the NASA Glenn Research Center at Lewis Field and under the guidance of U.S. principal investigator Professor John Hegseth of the University of New Orleans and three French coinvestigators Daniel Beysens, Yves Garrabos, and Carole Chabot. In early 1999, GMSF experiments were operated for 20 days on the Russian Space Station Mir. Mir astronauts performed experiments One through Seven, which spanned the three science themes of near-critical phase separation rates, interface dynamics in near-critical boiling, and measurement of the spectrum of density fluctuation length scales very close to the critical point. The fluids used were pure CO2 or SF6. Three of the five thermostats used could adjust the sample volume with the scheduled crew time. Such a volume adjustment enabled variable sample densities around the critical density as well as pressure steps (as distinct from the usual temperature steps) to be applied to the sample.

Wilkinson, R. Allen↗

NASA NTRS2004

Engineering concepts for inflatable Mars surface greenhouses

A major challenge of designing a bioregenerative life support system for Mars is the reduction of the mass, volume, power, thermal and crew-time requirements. Structural mass of the greenhouse could be saved by operating the greenhouse at low atmospheric pressure. This paper investigates the feasibility of this concept. The method of equivalent system mass is used to compare greenhouses operated at high atmospheric pressure to greenhouses operated at low pressure for three different lighting methods: natural, artificial and hybrid lighting. c2004 COSPAR. Published by Elsevier Ltd. All rights reserved.

NASA Discipline Life Support Systems↗

NASA NTRS2004

A status report on the characterization of the microgravity environment of the International Space Station

A primary objective of the International Space Station is to provide a long-term quiescent environment for the conduct of scientific research for a variety of microgravity science disciplines. Since continuous human presence on the space station began in November 2000 through the end of Increment-6, over 1260 hours of crew time have been allocated to research. However, far more research time has been accumulated by experiments controlled on the ground. By the end of the time period covered by this paper (end of Increment-6), the total experiment hours performed on the station are well over 100,000 hours (Expedition 6 Press Kit: Station Begins Third Year of Human Occupation, Boeing/USA/NASA, October 25, 2002). This paper presents the results of the on-going effort by the Principal Investigator Microgravity Services project, at NASA Glenn Research Center, in Cleveland, Ohio, to characterize the microgravity environment of the International Space Station in order to keep the microgravity scientific community apprised of the reduced gravity environment provided by the station for the performance of space experiments. This paper focuses on the station microgravity environment for Increments 5 and 6. During that period over 580 Gbytes of acceleration data were collected, out of which over 34,790 hours were analyzed. The results presented in this paper are divided into two sections: quasi-steady and vibratory. For the quasi-steady analysis, over 7794 hours of acceleration data were analyzed, while over 27,000 hours were analyzed for the vibratory analysis. The results of the data analysis are presented in this paper in the form of a grand summary for the period under consideration. For the quasi-steady acceleration response, results are presented in the form of a 95% confidence interval for the station during "normal microgravity mode operations" for the following three attitudes: local vertical local horizontal, X-axis perpendicular to the orbit plane and the Russian torque equilibrium attitude. The same analysis was performed for the station during "non-microgravity mode operations" to assess the station quasi-steady acceleration environment over a long period of time. The same type of analysis was performed for the vibratory, but a 95th percentile benchmark was used, which shows the overall acceleration magnitude during Increments 5 and 6. The results, for both quasi-steady and vibratory acceleration response, show that the station is not yet meeting the microgravity requirements during the microgravity mode operations. However, it should be stressed that the requirements apply only at assembly complete, whereas the results presented below apply up to the station's configuration at the end of Increment-6. c2004 Elsevier Ltd. All rights reserved.

ISS Project↗

NASA NTRS2001

Growth and Morphology of Supercritical Fluids, a Fluid Physics Experiment Conducted on Mir, Complete

The Growth and Morphology of Supercritical Fluids (GMSF) is an international experiment facilitated by the NASA Glenn Research Center and under the guidance of U.S. principal investor Professor Hegseth of the University of New Orleans and three French coinvestigators: Daniel Beysens, Yves Garrabos, and Carole Chabot. The GMSF experiments were concluded in early 1999 on the Russian space station Mir. The experiments spanned the three science themes of near-critical phase separation rates, interface dynamics in near-critical boiling, and measurement of the spectrum of density fluctuation length scales very close to the critical point. The fluids used were pure CO2 or SF6. Three of the five thermostats used could adjust the sample volume with the scheduled crew time. Such a volume adjustment enabled variable sample densities around the critical density as well as pressure steps (as distinct from the usual temperature steps) applied to the sample. The French-built ALICE II facility was used for these experiments. It allows tightly thermostated (left photograph) samples (right photograph) to be controlled and viewed/measured. Its diagnostics include interferometry, shadowgraph, high-speed pressure measurements, and microscopy. Data were logged on DAT tapes, and PCMCIA cards and were returned to Earth only after the mission was over. The ground-breaking near critical boiling experiment has yielded the most results with a paper published in Physical Review Letters (ref. 1). The boiling work also received press in Science Magazine (ref. 2). This work showed that, in very compressible near-critical two-phase pure fluids, a vapor bubble was induced to temporarily overheat during a rapid heating of the sample wall. The temperature rise in the vapor was 23-percent higher than the rise in the driving container wall. The effect is due to adiabatic compression of the vapor bubble by the rapid expansion of fluid near the boundary during heatup. Thermal diffusivity is low near the critical point, so getting heat out of the compressed bubble is observably slow. This gives the appearance of a backward heat flow, or heat flow from a cold surface to a warm fluid.

Wilkinson, R. Allen↗

NASA NTRS2004

Cardiovascular Countermeasures for Exploration-Class Space Flight Missions

Astronaut missions to Mars may be many years or even decades in thc future but current and planned efforts can be extrapolated to required treatments and prophylaxis for delerious efforts of prolonged space flight on the cardiovascular system. The literature of candidate countermeasures was considered in combination with unpublished plans for countermeasure implementation. The scope of cardiovascular countermeasures will be guided by assessments of the efficacy of mechanical, physiological and pharmacological approaches in protecting the cardiovascular capacities of interplanetary crewmembers. Plans for countermeasure development, evaluation and validation will exploit synergies among treatment modalities with the goal of maximizing protective effects while minimizing crew time and in-flight resource use. Protection of the cardiovascular capacity of interplanetary crewmembers will become more effective and efficient over the next few decades, but trade-offs between cost and effectiveness of efficiency are always possible if the increased level of risk can be accepted.

Charles, John B.↗

NASA NTRS2006

Habitability Designs for Crew Exploration Vehicle

NASA's space human factors team is contributing to the habitability of the Crew Exploration Vehicle (CEV), which will take crews to low Earth orbit, and dock there with additional vehicles to go on to the moon's surface. They developed a task analysis for operations and for self-sustenance (sleeping, eating, hygiene), and estimated the volumes required for performing the various tasks and for the associated equipment, tools and supplies. Rough volumetric mockups were built for crew evaluations. Trade studies were performed to determine the size and location of windows. The habitability analysis also contributes to developing concepts of operations by identifying constraints on crew time. Recently completed studies provided stowage concepts, tools for assessing lighting constraints, and approaches to medical procedure development compatible with the tight space and absence of gravity. New work will be initiated to analyze design concepts and verify that equipment and layouts do meet requirements.

Woolford, Barbara↗

NASA NTRS2004

The Art of Space Flight Exercise Hardware: Design and Implementation

The design of space flight exercise hardware depends on experience with crew health maintenance in a microgravity environment, history in development of flight-quality exercise hardware, and a foundation for certifying proper project management and design methodology. Developed over the past 40 years, the expertise in designing exercise countermeasures hardware at the Johnson Space Center stems from these three aspects of design. The medical community has steadily pursued an understanding of physiological changes in humans in a weightless environment and methods of counteracting negative effects on the cardiovascular and musculoskeletal system. The effects of weightlessness extend to the pulmonary and neurovestibular system as well with conditions ranging from motion sickness to loss of bone density. Results have shown losses in water weight and muscle mass in antigravity muscle groups. With the support of university-based research groups and partner space agencies, NASA has identified exercise to be the primary countermeasure for long-duration space flight. The history of exercise hardware began during the Apollo Era and leads directly to the present hardware on the International Space Station. Under the classifications of aerobic and resistive exercise, there is a clear line of development from the early devices to the countermeasures hardware used today. In support of all engineering projects, the engineering directorate has created a structured framework for project management. Engineers have identified standards and "best practices" to promote efficient and elegant design of space exercise hardware. The quality of space exercise hardware depends on how well hardware requirements are justified by exercise performance guidelines and crew health indicators. When considering the microgravity environment of the device, designers must consider performance of hardware separately from the combined human-in-hardware system. Astronauts are the caretakers of the hardware while it is deployed and conduct all sanitization, calibration, and maintenance for the devices. Thus, hardware designs must account for these issues with a goal of minimizing crew time on orbit required to complete these tasks. In the future, humans will venture to Mars and exercise countermeasures will play a critical role in allowing us to continue in our spirit of exploration. NASA will benefit from further experimentation on Earth, through the International Space Station, and with advanced biomechanical models to quantify how each device counteracts specific symptoms of weightlessness. With the continued support of international space agencies and the academic research community, we will usher the next frontier in human space exploration.

Beyene, Nahom M.↗

NASA NTRS2007

Eva Physiology, Systems, and Performance (EPSP) Project Overview

Extravehicular activity (EVA) is any activity performed by astronauts outside their space vehicle or habitat. EVA may be performed on orbit, such as outside the Space Shuttle or the International Space Station, or on a planetary surface such as Mars or on the moon. Astronauts wear a pressurized suit that provides environmental protection, mobility, life support, and communications while they work in the harsh conditions of a microgravity environment. Exploration missions to the moon and Mars may last many days and will include many types of EVAs; exploration, science, construction and maintenance. The effectiveness and success of these EVA-filled missions is dependent on the ability to perform tasks efficiently. The EVA Physiology, Systems and Performance (EPSP) project will conduct a number of studies to understand human performance during EVA, from a molecular level to full-scale equipment and suit design aspects, with the aim of developing safe and efficient systems for Exploration missions and the Constellation Program. The EPSP project will 1) develop Exploration Mission EVA suit requirements for metabolic and thermal loading, optional center of gravity location, biomedical sensors, hydration, nutrition, and human biomedical interactions; 2) develop validated EVA prebreathe protocols that meet medical, vehicle, and habitat constraints while minimizing crew time and thus increasing EVA work efficiency; and 3) define exploration decompression sickness (DCS) risks, policy, and mission success statistics and develop a DCS risk definition report.

Gernhardt, Michael L.↗

NASA NTRS2007

Potential Space Applications for Body-Centric Wireless and E-Textile Antennas

Space environment benefits of body-centric wireless communications are numerous, particularly in the context of long duration Lunar and Martian outposts that are in planning stages at several space agencies around the world. Since crew time for such missions is a scarce commodity, seamless integration of body-centric wireless from various sources is paramount. Sources include traditional data, such as audio, video, tracking, and biotelemetry. Newer data sources include positioning, orientation, and status of handheld tools and devices, as well as management and status of on-body inventories. In addition to offering lighter weight and flexibility, performance benefits of e-textile antennas are anticipated due to advantageous use of the body s surface area. In creating e-textile antennas and RF devices, researchers are faced with the challenge of transferring conventional and novel designs to textiles. Lack of impedance control, limited conductivity, and the inability to automatically create intricate designs are examples of limitations frequently attributed to e-textiles. Reliable interfaces between e-textiles and conventional hardware also represent significant challenges. Addressing these limitations is critical to the continued development and acceptance of fabric-based circuits for body-centric wireless applications. Here we present several examples of e-textile antennas and RF devices, created using a NASA-developed process, that overcome several of these limitations. The design and performance of an equiangular spiral, miniaturized spiral-loaded slot antenna, and a hybrid coupler are considered, with the e-textile devices showing comparable performance to like designs using conventional materials.

Kennedy, Timothy F.↗

NASA NTRS2007

Exploration Life Support Overview and Benefits

NASA s Exploration Life Support (ELS) Project is providing technology development to address air, water and waste product handling for future exploration vehicles. Existing life support technology and processes need to improve to enable exploration vehicles to meet mission goals. The weight, volume, power and thermal control required, reliability, crew time and life cycle cost are the primary targets for ELS technology development improvements. An overview of the ELS technologies being developed leads into an evaluation of the benefits the ELS technology developments offer.

Chambliss, Joe P.↗

NASA NTRS2005

Five Years of NASA Research on ISS: A Continuing Saga

The first NASA experiments reached ISS in September 2000, a very modest beginning to what later became a more robust, diverse and overall highly successful research program, continuing essentially uninterrupted since March 2001. Along the way, several major challenges had to be overcome. First, there were delays in the initial construction of the station. Second, maintenance of the station exceeded earlier assumptions resulting in less crew time being available for research. Third, the lengthy interruption of Shuttle flights after the Columbia accident significantly, but temporarily, reduced the research traffic to and from ISS. And fourth, the Vision of Space Exploration as caused a refocusing of NASA's research efforts on ISS from a multi-disciplinary basic and applied science program to one dedicated to solving the critical questions to enable exploration missions. The principal factors that allowed these challenges to be overcome have been flexibility and cooperation. Flexibility on the part of the ISS Program to minimize impacts to research from delays and resource bottlenecks, flexibility on the part of researchers to adapt their research to changing environments, and flexibility to be able to use existing and planned facilities not only for their original basic science purpose but also for new applications. And cooperation not only between the ISS Program and the research community, but also among NASA and its International Partners to continually strive to optimize the research conducted aboard ISS. Once the challenges were overcome, the research program has been remarkably successful, with an expanding on-orbit capability. Over 80 investigations have been completed, many resulting in publications.

Uri, John J.↗

NASA NTRS2005

Artificial Gravity: Will it Preserve Bone Health on Long-Duration Missions?

Prolonged microgravity exposure disrupts bone, muscle, and cardiovascular homeostasis, sensory-motor coordination, immune function, and behavioral performance. Bone loss, in particular, remains a serious impediment to the success of exploration-class missions by increasing the risks of bone fracture and renal stone formation for crew members. Current countermeasures, consisting primarily of resistive and aerobic exercise, have not yet proven fully successful for preventing bone loss during long-duration spaceflight. While other bone-specific countermeasures, such as pharmacological therapy and dietary modifications, are under consideration, countermeasure approaches that simultaneously address multiple physiologic systems may be more desirable for exploration-class missions, particularly if they can provide effective protection at reduced mission resource requirements (up-mass, power, crew time, etc). The most robust of the multi-system approaches under consideration, artificial gravity (AG), could prevent all of the microgravity-related physiological changes from occurring. The potential methods for realizing an artificial gravity countermeasure are reviewed, as well as selected animal and human studies evaluating the effects of artificial gravity on bone function. Future plans for the study of the multi-system effects of artificial gravity include a joint, cooperative international effort that will systematically seek an optimal prescription for intermittent AG to preserve bone, muscle, and cardiovascular function in human subjects deconditioned by 6 degree head-down-tilt-bed rest. It is concluded that AG has great promise as a multi-system countermeasure, but that further research is required to determine the appropriate parameters for implementation of such a countermeasure for exploration-class missions.

Davis-Street, Janis↗

NASA NTRS2007

Life Support System Technologies for NASA Exploration Missions

The Lunar Mars Life Support Test series successfully demonstrated integration and operation of advanced technologies for closed-loop life support systems, including physicochemical and biological subsystems. Increased closure was obtained when targeted technologies, such as brine dewatering subsystems, were added to further process life support system byproducts to recover resources. Physicochemical and biological systems can be integrated satisfactorily to achieve desired levels of closure. Imbalances between system components, such as differences in metabolic quotients between human crews and plants, must be addressed. Each subsystem or component that is added to increase closure will likely have added costs, ranging from initial launch mass, power, thermal, crew time, byproducts, etc., that must be factored into break even analysis. Achieving life support system closure while maintaining control of total mass and system complexity will be a challenge.

Ewert, Michael K.↗

NASA NTRS2007

Microbiological Monitoring for the Constellation Program: Current Requirements and Future Considerations

Microbiological requirements for spaceflight are based on assessments of infectious disease risk which could impact crew health or mission success. The determination of risk from infectious disease is composed of several factors including (1) crew susceptibility, (2) crew exposure to the infectious disease agent, (3) the concentration of the infectious agent, and (4) the characteristics of the infectious agent. As a result of the Health Stabilization Program, stringent monitoring, and cleaning protocols, in-flight environmental microbial monitoring is not necessary for short-duration spaceflights. However, risk factors change for long-duration missions, as exemplified by the presence of medically significant organisms in the environments of both the Mir and International Space Station (ISS). Based upon this historical evidence, requirements for short duration usage aboard the Orion Crew Exploration Vehicle and Lunar Lander Vehicle will not require in-flight monitoring; however, as mission duration increases with a Lunar Outpost, an ability to detect microbial hazard will be necessary. The nature of the detection requirements will depend on the maturity of technology in a rapidly evolving marketplace. Regardless, the hardware will still need to maximize information to discipline experts and the crew, while minimizing the size, mass, power consumption, and crew time usage. The refinement of these monitors will be a major goal in our efforts to travel successfully to Mars.

Ott, C. Mark↗

NASA NTRS2007

Foods for a Mission to Mars: Equivalent System Mass and Development of a Multipurpose Small-Scale Seed Processor

The candidate crops for planetary food systems include: wheat, white and sweet potatoes, soybean, peanut, strawberry, dry bean including le ntil and pinto, radish, rice, lettuce, carrot, green onion, tomato, p eppers, spinach, and cabbage. Crops such as wheat, potatoes, soybean, peanut, dry bean, and rice can only be utilized after processing, while others are classified as ready-to-eat. To process foods in space, the food processing subsystem must be capable of producing a variety of nutritious, acceptable, and safe edible ingredients and food produ cts from pre-packaged and resupply foods as well as salad crops grown on the transit vehicle or other crops grown on planetary surfaces. D esigning, building, developing, and maintaining such a subsystem is b ound to many constraints and restrictions. The limited power supply, storage locations, variety of crops, crew time, need to minimize waste , and other equivalent system mass (ESM) parameters must be considere d in the selection of processing equipment and techniques.

Gandolph, J.↗