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HAT-P-31b,c: A TRANSITING, ECCENTRIC, HOT JUPITER AND A LONG-PERIOD, MASSIVE THIRD BODY

We report the discovery of HAT-P-31b, a transiting exoplanet orbiting the V = 11.660 dwarf star GSC 2099-00908. HAT-P-31b is the first planet discovered with the Hungarian-made Automated Telescope (HAT) without any follow-up photometry, demonstrating the feasibility of a new mode of operation for the HATNet project. The 2.17 M{sub J} , 1.1 R{sub J} planet has a period of P{sub b} = 5.0054 days and maintains an unusually high eccentricity of e{sub b} = 0.2450 {+-} 0.0045, determined through Keck, FIbr-fed Echelle Spectrograph, and Subaru high-precision radial velocities (RVs). Detailed modeling of the RVs indicates an additional quadratic residual trend in the data detected to very high confidence. We interpret this trend as a long-period outer companion, HAT-P-31c, of minimum mass 3.4 M{sub J} and period {>=}2.8 years. Since current RVs span less than half an orbital period, we are unable to determine the properties of HAT-P-31c to high confidence. However, dynamical simulations of two possible configurations show that orbital stability is to be expected. Further, if HAT-P-31c has non-zero eccentricity, our simulations show that the eccentricity of HAT-P-31b is actively driven by the presence of c, making HAT-P-31 a potentially intriguing dynamical laboratory.

79 ASTRONOMY AND ASTROPHYSICS↗

The NASA-UC-UH Eta-Earth program. IV. A low-mass planet orbiting an M dwarf 3.6 PC from Earth

We report the discovery of a low-mass planet orbiting Gl 15 A based on radial velocities from the Eta-Earth Survey using HIRES at Keck Observatory. Gl 15 Ab is a planet with minimum mass Msin i = 5.35 ± 0.75 M {sub ⊕}, orbital period P = 11.4433 ± 0.0016 days, and an orbit that is consistent with circular. We characterize the host star using a variety of techniques. Photometric observations at Fairborn Observatory show no evidence for rotational modulation of spots at the orbital period to a limit of ∼0.1 mmag, thus supporting the existence of the planet. We detect a second RV signal with a period of 44 days that we attribute to rotational modulation of stellar surface features, as confirmed by optical photometry and the Ca II H and K activity indicator. Using infrared spectroscopy from Palomar-TripleSpec, we measure an M2 V spectral type and a sub-solar metallicity ([M/H] = –0.22, [Fe/H] = –0.32). We measure a stellar radius of 0.3863 ± 0.0021 R {sub ☉} based on interferometry from CHARA.

79 ASTRONOMY AND ASTROPHYSICS↗

Marine Corps Base Camp Lejeune Green Loss time series Zipcode

Critical military training and testing on lands along the nation’s coastal and estuarine shorelines are increasingly placed at risk because of development pressures in surrounding areas, impairments due to other anthropogenic disturbances, and increasing requirements for compliance with environmental regulations. The U.S. Department of Defense (DoD) intends to enhance and sustain its training and testing assets and to optimize its stewardship of natural resources through the development and application of an ecosystem-based management approach on DoD installations. To accomplish this goal, particularly for installations in estuarine/coastal environments, the Strategic Environmental Research and Development Program (SERDP) launched the Defense Coastal/Estuarine Research Program (DCERP) as a minimum 10-year effort at Marine Corps Base Camp Lejeune (MCBCL) in North Carolina. The results of the first six years of the program (DCERP1) are presented here.The overarching objectives of DCERP are to: (1) understand the effects of military training activities, infrastructure development, and other coastal military installations; (2) develop models, tools, and indicators to evaluate ecosystem health; and (3) recommend adaptive management strategies to sustain ecosystem natural resources within the context of an active military installation.Green Loss time series 'Zipcode' Input files are 5 pixel minimum area, MCBCL only data from the individual green loss change periods. These were converted from categories of intensity 2,3,4 to simple binary 'change' and 'no-change'. These files were then converted to the following: 84-90 loss: 0,1 84-98 loss: 0,10 84-05 loss: 0,100 84-07 loss: 0,1000 84-09 loss: 0,10000 Files then simply added together to create change time series. This results in a range of possible values from 11111 (loss detected during each time period) to 00001 (change in the 1984-1990 period, but not observed as green loss by 1998, 05,07 or 09 - probably a temporary impact that 'revegetated').

54 ENVIRONMENTAL SCIENCES↗

Marine Corps Base Camp Lejeune Green Gain time series Zipcode

Critical military training and testing on lands along the nation’s coastal and estuarine shorelines are increasingly placed at risk because of development pressures in surrounding areas, impairments due to other anthropogenic disturbances, and increasing requirements for compliance with environmental regulations. The U.S. Department of Defense (DoD) intends to enhance and sustain its training and testing assets and to optimize its stewardship of natural resources through the development and application of an ecosystem-based management approach on DoD installations. To accomplish this goal, particularly for installations in estuarine/coastal environments, the Strategic Environmental Research and Development Program (SERDP) launched the Defense Coastal/Estuarine Research Program (DCERP) as a minimum 10-year effort at Marine Corps Base Camp Lejeune (MCBCL) in North Carolina. The results of the first six years of the program (DCERP1) are presented here.The overarching objectives of DCERP are to: (1) understand the effects of military training activities, infrastructure development, and other coastal military installations; (2) develop models, tools, and indicators to evaluate ecosystem health; and (3) recommend adaptive management strategies to sustain ecosystem natural resources within the context of an active military installation.Green Gain time series 'Zipcode' Input files are 5 pixel minimum area, MCBCL only data from the individual green loss change periods. These were converted from categories of intensity 2,3,4 to simple binary 'change' and 'no-change'. These files were then converted to the following: 84-90 loss: 0,1 84-98 loss: 0,10 84-05 loss: 0,100 84-07 loss: 0,1000 84-09 loss: 0,10000 Files then simply added together to create change time series. This results in a range of possible values from 11111 (loss detected during each time period) to 00001 (change in the 1984-1990 period, but not observed as green loss by 1998, 05,07 or 09 - probably a temporary impact that 'revegetated').

54 ENVIRONMENTAL SCIENCES↗

Transduction-like gene transfer in the methanogen Methanococcus voltae

Strain PS of Methanococcus voltae (a methanogenic, anaerobic archaebacterium) was shown to generate spontaneously 4.4-kbp chromosomal DNA fragments that are fully protected from DNase and that, upon contact with a cell, transform it genetically. This activity, here called VTA (voltae transfer agent), affects all markers tested: three different auxotrophies (histidine, purine, and cobalamin) and resistance to BES (2-bromoethanesulfonate, an inhibitor of methanogenesis). VTA was most effectively prepared by culture filtration. This process disrupted a fraction of the M. voltae cells (which have only an S-layer covering their cytoplasmic membrane). VTA was rapidly inactivated upon storage. VTA particles were present in cultures at concentrations of approximately two per cell. Gene transfer activity varied from a minimum of 2 x 10(-5) (BES resistance) to a maximum of 10(-3) (histidine independence) per donor cell. Very little VTA was found free in culture supernatants. The phenomenon is functionally similar to generalized transduction, but there is no evidence, for the time being, of intrinsically viral (i.e., containing a complete viral genome) particles. Consideration of VTA DNA size makes the existence of such viral particles unlikely. If they exist, they must be relatively few in number;perhaps they differ from VTA particles in size and other properties and thus escaped detection. Digestion of VTA DNA with the AluI restriction enzyme suggests that it is a random sample of the bacterial DNA, except for a 0.9-kbp sequence which is amplified relative to the rest of the bacterial chromosome. A VTA-sized DNA fraction was demonstrated in a few other isolates of M. voltae.

Methanococcus/genetics/growth & development/metabo↗

Development and Implementation of Aseptic Operations for the MOMA-Mass Spectrometer

The ExoMars 2020 Rover is a life detection mission, and is classified as Planetary Protection (PP) Mission Category IVb, the first IVb mission since the Viking missions. Mars Organic Molecule Analyzer Mass Spectrometer (MOMA-MS) is a life detection instrument for the rover. To meet the stringent bioburden requirement of 0.03 spore/m2, the MS is subjected to Dry Heat Microbial Reduction (DHMR) to decrease the bioburden from a measured 88 spores/m2 to 0.009 spores/m2. After DHMR, exposure of the sample path must be kept to an absolute minimum and requires aseptic operations. Aseptic operations include determining the safe exposure time based on the surface area of exposure and particle fallout expected in the aseptic ISO class 5 workspace, preparing an aseptic ISO class 5 workspace, and using sterile garments and tools. During the exposure activity the environment is monitored with active and passive fallout for bioburden and real time airborne particle counts. Sterile tools are handled by a two person team so the operator touches only the tool and not the exterior surfaces of the sterilization pouch, and a sterile operating field is established as a safe place to organize tools or parts during the aseptic operations. In cases where aseptic operations are not feasible, localized DHMR is used after exposure. Any breach in the PP cleanliness can necessitate repeating instrument level DHMR, which not only has significant cost and schedule implications, but also is a risk to hardware that is not rated for repeated long exposures to high temperatures.

Aseptic processing↗

Assessment of Tritium Activities in Bailed versus Pumped Samples from Wells at the Nevada National Security Site

The Underground Test Area (UGTA) Activity uses a variety of methods to collect groundwater samples to identify radionuclide migration from underground nuclear tests. These include depth-discrete bailing, pumping with low-volume rod pumps, and pumping with electrical submersible pumps. The Nevada National Security Site (NNSS) Integrated Groundwater Sampling Plan specifies that when sampling with a pump, a minimum of three effective well volumes are withdrawn and then samples are collected after water-quality parameters have stabilized. In locations where pumping is not feasible, depth-discrete bailing is used and purging prior to sampling is usually not required. A recent study evaluated three sampling technologies and recommended that historical tritium results be evaluated where both pumped and bailed samples are available to identify preferred sampling protocols for the collection of tritium samples. The tritium ( 3 H) activities were obtained from the UGTA chemistry data base. Wells were identified with known 3 H activities above method detection limits, and then evaluated if both bailed and pumped samples had been collected. Twenty two wells and piezometers with bailed samples, pumped samples, and 3 H activities above background were identified for further consideration. The conclusions from this analysis are: Bailed samples collected for 3 H analysis near the water surface in a well are lower in 3 H activity than bailed samples from within screened intervals and pumped samples. Depth-discrete bailed samples from within the screened intervals are generally in good agreement with pumped samples from developed wells and piezometers. Depth-discrete bailed samples from undeveloped wells and piezometers are in good agreement with the first pumped samples. However, the next pumped samples increased in 3 H activity, resulting in a greater percent difference between the undeveloped bailed samples and later pumped samples. Continuous pumping over extended periods removing large purge volumes from wells can perturbate the surrounding groundwater system for long periods of time. These perturbations can cause large changes in 3 H activities in the aquifer near the well because of the mixing of groundwater with variable 3 H activities. Recommendations include: Bailed samples for 3 H should not be collected near the water surface in the well. Bailed samples should be collected from within the well screen. Logs of temperature, chemistry, and thermal flow should be evaluated to identify optimal depths within the well screen to collect depth-discrete bailer samples. Purging of large volumes of water from the well over extended periods of time should be avoided when collecting 3 H samples. Sufficient time should be allowed after pumping large volumes of water from the well (e.g., after well development) for the surrounding aquifer and 3 H activities to return to ambient conditions

54 ENVIRONMENTAL SCIENCES↗

EMIC waves and associated relativistic electron precipitation on 25-26 January 2013

Using measurements from the Van Allen Probes and the Balloon Array for RBSP Relativistic Electron Losses (BARREL), we perform a case study of electromagnetic ion cyclotron (EMIC) waves and associated relativistic electron precipitation (REP) observed on 25–26 January 2013. Among all the EMIC wave and REP events from the two missions, the pair of the events is the closest both in space and time. The Van Allen Probe-B detected significant EMIC waves at L = 2.1–3.9 and magnetic local time (MLT) = 21.0–23.4 for 53.5 min from 2353:00 UT, 25 January 2013. Meanwhile, BARREL-1T observed clear precipitation of relativistic electrons at L = 4.2–4.3 and MLT = 20.7–20.8 for 10.0 min from 2358 UT, 25 January 2013. Local plasma and field conditions for the excitation of the EMIC waves, wave properties, electron minimum resonant energy E min , and electron pitch angle diffusion coefficient D αα of a sample EMIC wave packet are examined along with solar wind plasma and interplanetary magnetic field parameters, geomagnetic activity, and results from the spectral analysis of the BARREL balloon observations to investigate the two types of events. The events occurred in the early main phase of a moderate storm (min. Dst* = -51.0 nT). The EMIC wave event consists of two parts. Finally, unlike the first part, the second part of the EMIC wave event was locally generated and still in its source region. It is found that the REP event is likely associated with the EMIC wave event.

58 GEOSCIENCES↗

STRUCTURE AND DYNAMICS OF THE 2010 JULY 11 ECLIPSE WHITE-LIGHT CORONA

The white-light corona (WLC) during the total solar eclipse on 2010 July 11 was observed by several teams in the Moon's shadow stretching across the Pacific Ocean and a number of isolated islands. We present a comparison of the WLC as observed by eclipse teams located on the Tatakoto Atoll in French Polynesia and on Easter Island, 83 minutes later, combined with near-simultaneous space observations. The eclipse was observed at the beginning of the solar cycle, not long after solar minimum. Nevertheless, the solar corona shows a plethora of different features (coronal holes, helmet streamers, polar rays, very faint loops and radial-oriented thin streamers, a coronal mass ejection, and a puzzling 'curtain-like' object above the north pole). Comparing the observations from the two sites enables us to detect some dynamic phenomena. The eclipse observations are further compared with a hairy-ball model of the magnetic field and near-simultaneous images from the Atmospheric Imaging Assembly on NASA's Solar Dynamics Observatory, the Extreme Ultraviolet Imager on NASA's Solar Terrestrial Relations Observatory, the Sun Watcher, using Active Pixel System Detector and Image Processing on ESA's PRoject for Onboard Autonomy, and the Naval Research Laboratory's Large Angle and Spectrometric Coronagraph on ESA's Solar and Heliospheric Observatory. The Ludendorff flattening coefficient is 0.156, matching the expected ellipticity of coronal isophotes at 2 Rs{sub un}, for this rising phase of the solar-activity cycle.

79 ASTRONOMY AND ASTROPHYSICS↗

A PLANETARY SYSTEM AROUND THE NEARBY M DWARF GJ 667C WITH AT LEAST ONE SUPER-EARTH IN ITS HABITABLE ZONE

We re-analyze 4 years of HARPS spectra of the nearby M1.5 dwarf GJ 667C available through the European Southern Observatory public archive. The new radial velocity (RV) measurements were obtained using a new data analysis technique that derives the Doppler measurement and other instrumental effects using a least-squares approach. Combining these new 143 measurements with 41 additional RVs from the Magellan/Planet Finder Spectrograph and Keck/High Resolution Echelle Spectrometer spectrometers reveals three additional signals beyond the previously reported 7.2 day candidate, with periods of 28 days, 75 days, and a secular trend consistent with the presence of a gas giant (period {approx}10 years). The 28 day signal implies a planet candidate with a minimum mass of 4.5 M{sub Circled-Plus} orbiting well within the canonical definition of the star's liquid water habitable zone (HZ), that is, the region around the star at which an Earth-like planet could sustain liquid water on its surface. Still, the ultimate water supporting capability of this candidate depends on properties that are unknown such as its albedo, atmospheric composition, and interior dynamics. The 75 day signal is less certain, being significantly affected by aliasing interactions among a potential 91 day signal, and the likely rotation period of the star at 105 days detected in two activity indices. GJ 667C is the common proper motion companion to the GJ 667AB binary, which is metal-poor compared to the Sun. The presence of a super-Earth in the HZ of a metal-poor M dwarf in a triple star system supports the evidence that such worlds should be ubiquitous in the Galaxy.

79 ASTRONOMY AND ASTROPHYSICS↗

SU-F-J-07: Evaluating the Adequacy of Biopsy Specimens for Genetic Signature Assessment by Measuring the Metabolic Activity in Specimens Obtained Under 18F-FDG PET/CT Guidance

Purpose: Genetic profiling of biopsied tissue is the basis for personalized cancer therapy. However biopsied materials may not contain sufficient amounts of DNA needed for analysis. We propose a method to determine the adequacy of specimens for performing genetic profiling by quantifying metabolic activity. Methods: We measured the response of two radiation detectors to the activity contained in the minimum amount of tumor cells needed for genetic profiling in biopsy specimens obtained under 2-deoxy-2-({sup 18}F)fluoro-D-glucose ({sup 18}F-FDG) PET/CT guidance. The expected tumor cell concentration in biopsy specimens was evaluated from the amount of DNA needed (∼100 µg) and the number of pathology sections typically used for the analysis. The average {sup 18}F-FDG uptake per cell was measured by incubating KPC-4662 pancreatic tumor cells and HT-29 colorectal adenocarcinoma tumor cells in {sup 18}F-FDG containing solution (activity concentrations between 0.0122 and 1.51 MBq/mL and glucose concentrations of 3.1 and 1 g/L) for 1 to 1.75 hours and then measuring the activity of a known number of cells. Measurements of surrogate specimens obtained using 18G needle biopsies of gels containing these cells in expected concentrations (∼10{sup 4} µL{sup −1}) were performed using an autoradiography CCD based device (up to 20 min exposure) and a scintillation well counter (∼1 min measurements) about 3 and 5 hours after the end of incubation respectively. Results: At start of autoradiography there were between 0.16 and 1.5 {sup 18}F-FDG molecules/cell and between 1.14 and 5.43×10{sup 7} {sup 18}F-FDG molecules/mL. For the scintillation well counter, sample to minimum-detectable-count rate ratios were greater than 7 and the counting error was less than 25% for ≤80 s measurement times. Images of the samples were identifiable on the autoradiograph for ∼10 min and longer exposure times. Conclusion: Scintillation well counter measurements and CCD based autoradiography have adequate sensitivity to detect the tumor burden needed for genetic profiling in 18G core needle biopsies. Supported in part through the NIH/NCI Cancer Center Support Grant P30 CA008748 and by a sponsored research agreement with Biospace Lab S.A.

60 APPLIED LIFE SCIENCES↗

Potential Activities and Long Lifetimes of Organic Carbon-Degrading Extracellular Enzymes in Deep Subsurface Sediments of the Baltic Sea

Heterotrophic microorganisms in marine sediments produce extracellular enzymes to hydrolyze organic macromolecules, so their products can be transported inside the cell and used for energy and growth. Therefore, extracellular enzymes may mediate the fate of organic carbon in sediments. The Baltic Sea Basin is a primarily depositional environment with high potential for organic matter preservation. The potential activities of multiple organic carbon-degrading enzymes were measured in samples obtained by the International Ocean Discovery Program Expedition 347 from the Little Belt Strait, Denmark, core M0059C. Potential maximum hydrolysis rates (V max ) were measured at depths down to 77.9mbsf for the following enzymes: alkaline phosphatase, β- d -xylosidase, β- d -cellobiohydrolase, N-acetyl-β- d -glucosaminidase, β-glucosidase, α-glucosidase, leucyl aminopeptidase, arginyl aminopeptidase, prolyl aminopeptidase, gingipain, and clostripain. Extracellular peptidase activities were detectable at depths shallower than 54.95mbsf, and alkaline phosphatase activity was detectable throughout the core, albeit against a relatively high activity in autoclaved sediments. β-glucosidase activities were detected above 30mbsf; however, activities of other glycosyl hydrolases (β-xylosidase, β-cellobiohydrolase, N-acetyl-β-glucosaminidase, and α-glucosidase) were generally indistinguishable from zero at all depths. These extracellular enzymes appear to be extremely stable: Among all enzymes, a median of 51.3% of enzyme activity was retained after autoclaving for an hour. We show that enzyme turnover times scale with the inverse of community metabolic rates, such that enzyme lifetimes in subsurface sediments, in which metabolic rates are very slow, are likely to be extraordinarily long. A back-of-the-envelope calculation suggests enzyme lifetimes are, at minimum, on the order of 230days, and may be substantially longer. These results lend empirical support to the hypothesis that a population of subsurface microbes persist by using extracellular enzymes to slowly metabolize old, highly degraded organic carbon.

54 ENVIRONMENTAL SCIENCES↗

Bootstrap inversion technique for atmospheric trace gas source detection and quantification using long open-path laser measurements

Advances in natural gas extraction technology have led to increased activity in the production and transport sectors in the United States and, as a consequence, an increased need for reliable monitoring of methane leaks to the atmosphere. We present a statistical methodology in combination with an observing system for the detection and attribution of fugitive emissions of methane from distributed potential source location landscapes such as natural gas production sites. We measure long (> 500 m), integrated open-path concentrations of atmospheric methane using a dual frequency comb spectrometer and combine measurements with an atmospheric transport model to infer leak locations and strengths using a novel statistical method, the non-zero minimum bootstrap (NZMB). The new statistical method allows us to determine whether the empirical distribution of possible source strengths for a given location excludes zero. Using this information, we identify leaking source locations (i.e., natural gas wells) through rejection of the null hypothesis that the source is not leaking. The method is tested with a series of synthetic data inversions with varying measurement density and varying levels of model–data mismatch. It is also tested with field observations of (1) a non-leaking source location and (2) a source location where a controlled emission of 3.1 × 10 -5 kg s -1 of methane gas is released over a period of several hours. This series of synthetic data tests and outdoor field observations using a controlled methane release demonstrates the viability of the approach for the detection and sizing of very small leaks of methane across large distances (4 + km 2 in synthetic tests). The field tests demonstrate the ability to attribute small atmospheric enhancements of 17 ppb to the emitting source location against a background of combined atmospheric (e.g., background methane variability) and measurement uncertainty of 5 ppb (1 σ ), when measurements are averaged over 2 min. The results of the synthetic and field data testing show that the new observing system and statistical approach greatly decreases the incidence of false alarms (that is, wrongly identifying a well site to be leaking) compared with the same tests that do not use the NZMB approach and therefore offers increased leak detection and sizing capabilities.

03 NATURAL GAS↗

Activity Cycles in Stars

Starspots and stellar activity can be detected in other stars using high precision photometric and spectrometric measurements. These observations have provided some surprises (starspots at the poles - sunspots are rarely seen poleward of 40 degrees) but more importantly they reveal behaviors that constrain our models of solar-stellar magnetic dynamos. The observations reveal variations in cycle characteristics that depend upon the stellar structure, convection zone dynamics, and rotation rate. In general, the more rapidly rotating stars are more active. However, for stars like the Sun, some are found to be inactive while nearly identical stars are found to be very active indicating that periods like the Sun's Maunder Minimum (an inactive period from 1645 to 1715) are characteristic of Sun-like stars.

Hathaway, David H.↗

Performance Evaluation of Spectroscopic Detectors for LEU Hold-up Measurements

The hold-up measurement of low-enriched uranium materials may require use of alternate detector types relative to the measurement of highly enriched uranium. This is in part due to the difference in process scale (i.e., the components are generally larger for low-enriched uranium systems), but also because the characteristic gamma-ray lines from 235 U used for assay of highly enriched uranium will be present at a much reduced intensity (on a per gram of uranium basis) at lower enrichments. Researchers at Oak Ridge National Laboratory examined the performance of several standard detector types, e.g., NaI(Tl), LaBr3(Ce), and HPGe, to select a suitable candidate for measuring and quantifying low-enriched uranium hold-up in process pipes and equipment at the Portsmouth gaseous diffusion plant. Detector characteristics, such as energy resolution (full width at half maximum) and net peak count rates at gamma ray energies spanning a range of 60–1332 keV, were measured for the above-mentioned detector types using the same sources and in the same geometry. Uranium enrichment standards (Certified Reference Material no. 969 and Certified Reference Material no. 146) were measured using each of the detector candidates in the same geometry. The net count rates recorded by each detector at 186 keV and 1,001 keV were plotted as a function of enrichment (atom percentage). Background measurements were made in unshielded and shielded configurations under both ambient and elevated conditions of 238 U activity. The highly enriched uranium hold-up measurement campaign at the Portsmouth plant was performed on process equipment that had been cleaned out. Therefore, in most cases, the thickness of the uranium deposits was less than the “infinite thickness” for the 186 keV gamma rays to be completely self-attenuated. Because of this, in addition to measuring the 186 keV gamma, the 1,001 keV gamma ray from 234m Pa—a daughter of 238 U in secular equilibrium with its parent—will also need to be measured. Based on the performance criteria of detection efficiency, energy resolution, peak-to-continuum ratios, minimum detectable limits, and the weight of the shielded probe, a shielded (0.5 in. thick lead shield) 2 × 2 in. NaI(Tl) detector is recommended for use. The recommended approach is to carry out analysis using data from both 186 keV and 1,001 keV gamma rays, and select a best result based on propagated uncertainty estimates. It is also highly recommended that a two-point gain stabilization scheme based on an 241 Am seed embedded in the probe be implemented. Shielding configurations to reduce the impact of background interference on the measurement of 1,001 keV gamma-ray are discussed.

46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND ↗

A Coupled Approach for Structural Damage Detection with Incomplete Measurements

This historical work couples model order reduction, damage detection, dynamic residual/mode shape expansion, and damage extent estimation to overcome the incomplete measurements problem by using an appropriate undamaged structural model. A contribution of this work is the development of a process to estimate the full dynamic residuals using the columns of a spring connectivity matrix obtained by disassembling the structural stiffness matrix. Another contribution is the extension of an eigenvector filtering procedure to produce full-order mode shapes that more closely match the measured active partition of the mode shapes using a set of modified Ritz vectors. The full dynamic residuals and full mode shapes are used as inputs to the minimum rank perturbation theory to provide an estimate of damage location and extent. The issues associated with this process are also discussed as drivers of near-term development activities to understand and improve this approach.

James, George↗

Effect of betulinic acid and its ionic derivatives on M-MuLV replication

Murine leukemia virus (MuLV) is a retrovirus known causing leukemia and neurological disorders in mice, and its viral life cycle and pathogenesis have been investigated extensively over the past decades. As a natural antiviral agent, betulinic acid is a pentacyclic triterpenoid that can be found in the bark of several species of plants (particularly the white birch). One of the hurdles for betulinic acid to release its antiviral potency is its poor water solubility. In this study, we synthesized more water-soluble ionic derivatives of betulinic acid, and examined their activities against Moloney MuLV (M-MuLV). The mouse fibroblast cells stably infected with M-MuLV, 43D cells, were treated with various doses of betulinic acids and its derivatives, and the viral structural protein Gag in cells and media were detected by western blots. Two ionic derivatives containing the benzalkonium cation were found to inhibit the virus production into media and decreased Gag in cells. However, a cell proliferation assay showed that the benzalkonium cation inhibited the growth of 43D cells, suggesting that our ionic derivatives limited virus production through the inhibition of metabolism in 43D cells. Interestingly, all of these betulinic acid compounds exhibited a minimum impact on the processing and release of Gag from 43D cells, which outlines the differences of viral maturation between MuLV and human immunodeficiency virus.

60 APPLIED LIFE SCIENCES↗

Electrification in Hurricanes over the Tropical Americas: Implication for Stratospheric Water Vapor

This study explores the relation between lightning activity and water vapor in the Tropical Tropopause Layer (TTL) over hurricane systems in the Tropical Americas. The hypothesis herein is that hurricanes that exhibit enhanced lightning activity are associated with stronger updrafts that can transport more moisture directly into the TTL (and subsequently into the tropical stratosphere) or even directly into the tropical stratosphere over this region. The TTL over the Tropical Americas, which includes the Caribbean and Gulf of Mexico, is of particular interest, because summertime cold point tropopause is the lowest in height and thus the warmest in temperature over the tropics. The latter condition implies higher saturation values and thus potential for more water vapor to enter the stratosphere. Climate forecast is very sensitive to stratospheric water vapor abundance, because of the key role that water vapor plays in regulating the chemical and radiative properties of the stratosphere. Given the potential for increases in hurricane intensity and frequency under predicted warmer conditions, it becomes essential to understand the effect of hurricanes on stratospheric water vapor. In this study, we use a combination of ground and space-borne observations as well as trajectory calculations. The observations include: cloud-to-ground (CG) lightning data from the U.S. National Lightning Detection Network (NLDN), geostationary infrared observations from the National Climatic Data Center Hurricane Satellite (HURSAT) data set, cloud properties from Aqua-MODIS, and water vapor from Aura-MLS. We analyze hurricanes from the 2005 season when Aura-MLS data are available, namely: Dennis, Emily, Katrina, Rita, and Wilma. Our analysis consists of examining CG lightning, cloud-top properties, and TTL water vapor (i.e., 100 and 147 mb) over the hurricane while it remains over water in the Tropical Americas region. We investigate daily as well as diurnal statistical properties. The hurricanes analyzed in this study showed that lightning activity is negatively correlated with minimum infrared brightness temperature and positively correlated with 100-mb water vapor. An examination of the maxima in water vapor observed over the hurricane not only shows larger magnitudes, but also larger differences between water vapor averages and water vapor maxima over the hurricane as lightning activity increases. Trajectory calculations are performed using the Flextra model in order to investigate the fate of the moister air masses found in the TTL.

Pittman, Jasna V.↗