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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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At least 217 records · Page 12

Regulation of Cell Fate Determination by Single-Repeat R3 MYB Transcription Factors in Arabidopsis

MYB transcription factors regulate multiple aspects of plant growth and development. Among the large family of MYB transcription factors, single-repeat R3 MYB are characterized by their short sequence (<120 amino acids) consisting largely of the single MYB DNA-binding repeat. In the model plant Arabidopsis, R3 MYBs mediate lateral inhibition during epidermal patterning and are best characterized for their regulatory roles in trichome and root hair development. R3 MYBs act as negative regulators for trichome formation but as positive regulators for root hair development. In this article, we provide a comprehensive review on the role of R3 MYBs in the regulation of cell type specification in the model plant Arabidopsis.

cell fate determination↗

CCS2, an Octatricopeptide-Repeat Protein, Is Required for Plastid Cytochrome c Assembly in the Green Alga Chlamydomonas reinhardtii

In bacteria and energy generating organelles, c -type cytochromes are a class of universal electron carriers with a heme cofactor covalently linked via one or two thioether bonds to a heme binding site. The covalent attachment of heme to apocytochromes is a catalyzed process, taking place via three evolutionarily distinct assembly pathways (Systems I, II, III). System II was discovered in the green alga Chlamydomonas reinhardtii through the genetic analysis of the ccs mutants (cytochrome c synthesis), which display a block in the apo- to holo- form conversion of cytochrome f and c6, the thylakoid lumen resident c-type cytochromes functioning in photosynthesis. Here we show that the gene corresponding to the CCS2 locus encodes a 1,719 amino acid polypeptide and identify the molecular lesions in the ccs2-1 to ccs2-5 alleles. The CCS2 protein displays seven degenerate amino acid repeats, which are variations of the octatricopeptide-repeat motif (OPR) recently recognized in several nuclear-encoded proteins controlling the maturation, stability, or translation of chloroplast transcripts. A plastid site of action for CCS2 is inferred from the finding that GFP fused to the first 100 amino acids of the algal protein localizes to chloroplasts in Nicotiana benthamiana . Here, we discuss the possible functions of CCS2 in the heme attachment reaction.

59 BASIC BIOLOGICAL SCIENCES↗

Row selection in remote sensing from four-row plots of maize and sorghum based on repeatability and predictive modeling

Remote sensing enables the rapid assessment of many traits that provide valuable information to plant breeders throughout the growing season to improve genetic gain. These traits are often extracted from remote sensing data on a row segment (rows within a plot) basis enabling the quantitative assessment of any row-wise subset of plants in a plot, rather than a few individual representative plants, as is commonly done in field-based phenotyping. Nevertheless, which rows to include in analysis is still a matter of debate. The objective of this experiment was to evaluate row selection and plot trimming in field trials conducted using four-row plots with remote sensing traits extracted from RGB (red-green-blue), LiDAR (light detection and ranging), and VNIR (visible near infrared) hyperspectral data. Uncrewed aerial vehicle flights were conducted throughout the growing seasons of 2018 to 2021 with data collected on three years of a sorghum experiment and two years of a maize experiment. Traits were extracted from each plot based on all four row segments (RS) (RS1234), inner rows (RS23), outer rows (RS14), and individual rows (RS1, RS2, RS3, and RS4). Plot end trimming of 40 cm was an additional factor tested. Repeatability and predictive modeling of end-season yield were used to evaluate performance of these methodologies. Plot trimming was never shown to result in significantly different outcomes from non-trimmed plots. Significant differences were often observed based on differences in row selection. Plots with more row segments were often favorable for increasing repeatability, and excluding outer rows improved predictive modeling. These results support long-standing principles of experimental design in agronomy and should be considered in breeding programs that incorporate remote sensing.

59 BASIC BIOLOGICAL SCIENCES↗

A NEUTRON STAR–WHITE DWARF BINARY MODEL FOR REPEATING FAST RADIO BURST 121102

We propose a compact binary model for the fast radio burst (FRB) repeaters, where the system consists of a magnetic white dwarf (WD) and a neutron star (NS) with strong bipolar magnetic fields. When the WD fills its Roche lobe, mass transfer will occur from the WD to the NS through the inner Lagrange point. The accreted magnetized materials may trigger magnetic reconnection when they approach the NS surface, and therefore the electrons can be accelerated to an ultra-relativistic speed. In this scenario, the curvature radiation of the electrons moving along the NS magnetic field lines can account for the characteristic frequency and the timescale of an FRB. Owing to the conservation of angular momentum, the WD may be kicked away after a burst, and the next burst may appear when the system becomes semi-detached again through the gravitational radiation. By comparing our analyses with the observations, we show that such an intermittent Roche-lobe overflow mechanism can be responsible for the observed repeating behavior of FRB 121102.

79 ASTRONOMY AND ASTROPHYSICS↗

Repeating FRB 121102: Eight-year Fermi-LAT Upper Limits and Implications

The repeating fast radio burst (FRB) source that produced FRB 121102 was recently localized in a star-forming galaxy at z = 0.193, which is associated with an extended radio source at the burst location. One possibility is that the repeating FRBs are produced by a new-born magnetar, which also powers the radio nebula. If so, the magnetar may produce γ-ray emission due to magnetic dipolar spin-down. The luminosity depends on the magnetar spin parameters and age. We process the eight-year Fermi-LAT data at the position of FRB 121102 and place an energy flux upper limit of ∼10{sup −11} erg cm{sup −2} s{sup −1} in time bins with six-month intervals, and an accumulated energy flux upper limit of ∼4×10{sup −12} erg cm{sup −2} s{sup −1} over the eight-year span. The corresponding γ-ray luminosity upper limits are ∼10{sup 45} erg s{sup −1} and ∼4×10{sup 44} erg s{sup −1} for the time-resolved and time-integrated analyses, respectively. We discuss the implications of these limits on the young magnetar model.

79 ASTRONOMY AND ASTROPHYSICS↗

RNA from a simple-tandem repeat is required for sperm maturation and male fertility in Drosophila melanogaster

Tandemly-repeated DNAs, or satellites, are enriched in heterochromatic regions of eukaryotic genomes and contribute to nuclear structure and function. Some satellites are transcribed, but we lack direct evidence that specific satellite RNAs are required for normal organismal functions. Here, we show satellite RNAs derived from AAGAG tandem repeats are transcribed in many cells throughout Drosophila melanogaster development, enriched in neurons and testes, often localized within heterochromatic regions, and important for viability. Strikingly, we find AAGAG transcripts are necessary for male fertility, and that AAGAG RNA depletion results in defective histone-protamine exchange, sperm maturation and chromatin organization. Since these events happen late in spermatogenesis when the transcripts are not detected, we speculate that AAGAG RNA in primary spermatocytes ‘primes’ post-meiosis steps for sperm maturation. In addition to demonstrating essential functions for AAGAG RNAs, comparisons between closely related Drosophila species suggest that satellites and their transcription evolve quickly to generate new functions.

59 BASIC BIOLOGICAL SCIENCES↗

Multiple-beam satellite repeater tradeoffs applied to a multifunctional system.

This paper concerns satellite repeater tradeoffs for a multiple-beam, multiple-channel, multiple-connectivity satellite postulated to serve a diversity of domestic U.S. noncommercial requirements, with a substantial portion of the satellite power, weight, and bandwidth dedicated to video distribution and bradcast. The orders-of-magnitude of the requirements are given for the video plus high-rate data traffic category. The designs of two alternative baseline repeater configurations are presented, and estimates of their weight and their prime power consumption are given. These data are the basis for estimating the weight and power consumption of a range of alternative beam coverage, modulation, and channel capacity systems. These estimates are used to illustrate tradeoffs with different performance criteria as the independent variable: number of channels per beam and total number of beam-channels.

Kinal, G. V.↗

Repeatability of elevation measurements: Apollo photography

Sun elevation angle effects on repeatability, using Apollo 15 photographs are analyzed and results extended to slope related effects. Preliminary results indicate repeatibility of elevation measurement is related to contrast in the stereoscopic image.

Wu, S. S. C.↗

Evaluation of pulsed RFI effects on digital satellite repeaters

This paper presents an analytical approach for assessing the effect of pulsed RFI on the error probability of a coherent phase-shift keyed signal through a nonlinear satellite repeater. The RFI is assumed to affect the uplink channel and to consist of CW pulses with random power levels and arriving randomly in time with a Poisson distribution. A model to approximate the effect of intermodulation products is introduced and the error probability conditioned on the output of the satellite repeater is computed. The classical moment technique is then used as an efficient method of averaging the conditional error probability over the numerous random parameters associated with the uplink signal.

Huang, T. C.↗

Spatial resolution and repeatability of Magsat crustal anomaly data over the Indian Ocean

Preliminary results from analysis of individual Magsat tracks in the eastern Indian Ocean demonstrate the resolution capability and repeatability of these data. Spectrum analysis indicates that 250 km is the absolute lower limit for resolving crustal magnetic anomalies in Magsat along-track data. Spectral coherence analysis of closely-spaced tracks shows that magnetic anomaly features with wavelengths greater than 700 km are highly repeatable from track to track.

Sailor, R. V.↗

Packet communication system for a multi-beam beam switched satellite repeater

This paper presents the design of a packet-switched communication system using a multi-beam, beam switched satellite repeater. The protocol provides a combination of random access via Slotted ALOHA techniques and demand assigned access using collision requests. This allows efficient bandwidth usage and low average delays. Minimization of earth station cost was a major objective. This was achieved by transferring the bulk of the system complexity to the satellite repeater and the (ground) network controller. This centrally controlled protocol would allow greater system stability and would permit system reconfiguration in response to changes in traffic intensity. Sufficient signalling is also incorporated to allow both rate diversity to combat fading and timing corrections to account for satellite drift.

Bose, S. K.↗

Positional repeatability measurements of stepper motors at cryogenic temperatures

For cryogenically-cooled infrared instruments and telescopes employed in space, reliable and predictable motor operations at 4 K in vacuum are in most cases necessary. For the Shuttle Infrared Telescope Facility (SIRTF), stepper motors may be employed in a number of applications. A test facility was designed and built for measuring stepper motor positional repeatability, in vacuum, over a temperature range from ambient to liquid helium temperature. Tests regarding positional repeatability were conducted at different motor speeds, directions of motor rotation, step sizes, and power conditions. In addition, tests were performed with respect to the ability of the motor to remain in a position with no power applied to the motor.

Pompea, S. M.↗

Repeatable electrical measurement instrumentation for use in the accelerated stress testing of thin film solar cells

Attention is given to the construction, calibration, and performance of a repeatable measurement system for use in conjunction with the accelerated stress testing of a-Si:H cells. A filtered diode array is utilized to approximate the spectral response of any type of solar cell in discrete portions of the spectrum. It is noted that in order to achieve the necessary degree of overall repeatability, it is necessary to pay particular attention to methods of contacting and positioning the cells.

Davis, C. W.↗

Topex orbit determination and gravity recovery using GPS data from repeat orbits

A covariance analysis is presented for a differential GPS-based technique for satellite tracking and gravity recovery to be demonstrated on Topex in the early 1990's. The technique employs data from an ensemble of repeat ground tracks to recover a unique satellite epoch state for each track and a set of invariant local gravity parameters common to all tracks. For satellites above about 800 km altitude, where gravity modeling is the dominant systematic error, averaging of random error over many arcs and adjustment of the gravity model reduces the final satellite position error. The local gravity parameters can then be used to produce a refined global gravity model. The analysis indicates that errors ranging from 5 to 8 cm in Topex altitude and 0.05 to 0.2 mgal for the gravity field can be achieved, depending on the number of repeat arcs used.

Wu, Jiun-Tsong↗

The strength of laminated composite materials under repeated impact loading

When low velocity and energy impact is exerted on a laminated composite material, in a perpendicular direction to the plane of the laminate, invisible damage may develop. It is shown analytically and experimentally that the invisible damage occurs during the first stage of contact between the impactor and the laminate and is a result of the contact stresses. However, the residual flexural strength changes only slightly, because it depends mainly on the outer layers, and these remain undamaged. Repeated impact intensifies the damage inside the laminate and causes larger bending under equivalent impact load. Finally, when the damage is most severe, even though it is still invisible, the laminate fails because of bending on the tension side. If the repeated impact is halted before final fracture occurs the residual strength and modulus would decrease by a certain amount.

Rotem, Assa↗

Eigenvector derivatives of repeated eigenvalues using singular value decomposition

An explicit formula is obtained for the first-order eigenvector derivative that corresponds to the eigenvector of a repeated eigenvalue, in the case of the nonself-adjoint eigenvalue problem. This method applies to the class of nondefective problems whose first eigenvalue derivatives of the repeated eigenvalues are distinct. A singular-value decomposition approach is used to compute four requisite bases for eigenspaces, as well as to keep track of the dimensions of state variables and the conditioning of the state equations.

Lim, Kyong B.↗

The inclusion of higher degree and order gravity terms in the design of a repeat ground track orbit

Mean Orbit Elements can be chosen such that a desired ground track repeat criteria is obtained when propagating with the secular effects of a central body gravity field. If the proper initial conditions are chosen that correspond to these Mean Elements, the orbit can also be propagated with the full gravity field and form another ground track repeat pattern. Further, if the effects of other perturbative forces are compensated for, such that the ground track remains near this pattern, the central body effects can again be considered repetitive. The process of arriving at these conclusions, along with the supporting numerical simulations, are presented.

Vincent, Mark A.↗

Eigenvalue and eigenvector sensitivity and approximate analysis for repeated eigenvalue problems

A set of computationally efficient equations for eigenvalue and eigenvector sensitivity analysis are derived, and a method for eigenvalue and eigenvector approximate analysis in the presence of repeated eigenvalues is presented. The method developed for approximate analysis involves a reparamaterization of the multivariable structural eigenvalue problem in terms of a single positive-valued parameter. The resulting equations yield first-order approximations of changes in both the eigenvalues and eigenvectors associated with the repeated eigenvalue problem. Examples are given to demonstrate the application of such equations for sensitivity and approximate analysis.

Hou, Gene J. W.↗