The fluid mechanics of continuous flow electrophoresis in perspective
Buoyancy alters the flow in continuous flow electrophoresis chambers through the mechanism of hydrodynamic instability and, when the instability is supressed by careful cooling of the chamber boundaries, by restructuring the axial flow. The expanded roles of buoyancy follow upon adapting the size of the chamber and the electric field so as to fractionate certain sorts of cell populations. Scale-up problems, hydrodynamic stability and the altered flow fields are discussed to show how phenomena overlooked in the design and operations of narrow-gap devices take on an overwhelming importance in wide-gap chambers