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At least 217 records · Page 12

Repeated freeze-thaw cycles increase extractable, but not total, carbon and nitrogen in a Maine coniferous soil

Northeastern North America has been experiencing warmer winters with reduced snow accumulation, with more frequent winter freeze-thaw cycles. We conducted a laboratory experiment to investigate how increased frequency of freeze-thaw cycles (FTC) would alter soil C and N availability. Organic (O) and mineral (B) horizon soils were collected from a coniferous forest in Maine, processed to exclude roots, and then frozen in the laboratory (-10 °C) with one (FTC-1), two (FTC-2), or six (FTC-6) thaw periods. Soils were analyzed for extractable inorganic ammonium (NH4-N), water extractable organic carbon (WEOC), carbon dioxide flux (respiration), and total C and N. Extractable NH4-N increased following FTC, for both horizons. WEOC in O horizons had a lower SUVA254, indicating a stronger microbial influence (i.e., microbial cell lysis) in these soils after FTC. Respiration in O horizon soils decreased post-incubation and did not differ between FTC and Control soils. In the B horizon, however, FTC soils showed greater respiration than Control soils, suggesting that the newly available nutrients may have stimulated microbial activity. In contrast to these results, total C and N remained unaltered by FTC, presumably because the FTC disturbances represented mostly a translocation of C and N from one pool into another, and losses due to respiration were too small to significantly influence the large TC and TN pools. The effect of FTC on NH4-N did not change with FTC frequency, suggesting that a single FTC is sufficient to alter both C and N availability and quality, and that additional FTC may not have a significant further effect. This study provides fresh insights on how organic and mineral soils might respond to increased freeze-thaw frequency in winter.

Patel, Kaizad F.↗

Experimental Soil Warming Impacts Soil Moisture and Plant Water Stress and Thereby Ecosystem Carbon Dynamics (Blodgett, CA)

This dataset contains data on daily soil temperature, moisture and flux, and soil carbon stock and root biomass across a soil profile down to 100 cm depth at Blodgett Forest Research Station, CA, USA. These data were generated to determine if modeling of an experimental soil warming of 4C showed increased soil CO2 emissions and changes in bulk soil carbon stocks with depth consistent with field observations, as part of the study: Riley et al. (2025) Experimental Soil Warming Impacts Soil Moisture and Plant Water Stress and Thereby Ecosystem Carbon Dynamics in Journal of Advances in Modeling Earth Systems. This research was performed within the framework of the TES Belowground Biogeochemistry SFA project, in particular association with a 1 m-deep experimental soil heating experiment at the University of California Blodgett Forest Research Station, California (120 ° 39′40′′W; 38 ° 54′43′′N). Continuous data were collected at the plot level, and bulk soil carbon and root biomass were sampled once a year from each plot from 0-100 cm, in 10 cm intervals. Measurements relevant to the current study include soil temperature and soil volumetric water content measured continuously at multiple depths in the top meter; fine root biomass and SOC stocks measured from annual soil cores. Soil flux was continuously monitored using a LI-8100 Automated CO2 Flux System in conjunction with the LI-8150 Multiplexer (Licor, Nebraska, USA). Soil flux was determined using SoilFluxPro software, with flux values showing an R² fit of less than 0.9 being excluded from the analysis. Data were collected from each paired plot (1-3): one control (C) and one heated (H).

54 ENVIRONMENTAL SCIENCES↗

Effects of ethylene on the kinetics of curvature and auxin redistribution in gravistimulated roots of Zea mays

We tested the involvement of ethylene in maize (Zea mays L.) root gravitropism by measuring the kinetics of curvature and lateral auxin movement in roots treated with ethylene, inhibitors of ethylene synthesis, or inhibitors of ethylene action. In the presence of ethylene the latent period of gravitropic curvature appeared to be increased somewhat. However, ethylene-treated roots continued to curve after control roots had reached their final angle of curvature. Consequently, maximum curvature in the presence of ethylene was much greater in ethylene-treated roots than in controls. Inhibitors of ethylene biosynthesis or action had effects on the kinetics of curvature opposite to that of ethylene, i.e. the latent period appeared to be shortened somewhat while total curvature was reduced relative to that of controls. Label from applied 3H-indole-3-acetic acid was preferentially transported toward the lower side of stimulated roots. In parallel with effects on curvature, ethylene treatment delayed the development of gravity-induced asymmetric auxin movement across the root but extended its duration once initiated. The auxin transport inhibitor, 1-N-naphthylphthalamic acid reduced both gravitropic curvature and the effect of ethylene on curvature. Since neither ethylene nor inhibitors of ethylene biosynthesis or action prevented curvature, we conclude that ethylene does not mediate the primary differential growth response causing curvature. Because ethylene affects curvature and auxin transport in parallel, we suggest that ethylene modifies curvature by affecting gravity-induced lateral transport of auxin, perhaps by interfering with adaptation of the auxin transport system to the gravistimulus.

Non-NASA Center↗

Larch (Larix dahurica Turcz) growth response to climate change in the Siberian permafrost zone

Larch-dominant communities are the most extensive high-latitude forests in Eurasia and are experiencing the strongest impacts from warming temperatures. We analyzed larch (Larix dahurica Turcz) growth index (GI) response to climate change. The studied larch-dominant communities are located within the permafrost zone of Northern Siberia at the northern tree limit (ca. N 67° 38′, E 99° 07′). Methods included dendrochronology, analysis of climate variables, root zone moisture content, and satellitederived gross (GPP) and net (NPP) primary productivity. It was found that larch response to warming included a period of increased annual growth increment (GI) (from the 1970s to ca. 1995) with a follow on GI decline. Increase in GI correlated with summer air temperature, whereas an observed decrease in GI was caused by water stress (vapor pressure deficit and drought increase). Water stress impact on larch growth in permafrost was not observed before the onset of warming (ca. 1970). Water limitation was also indicated by GI dependence on soil moisture stored during the previous year. Water stress was especially pronounced for stands growing on rocky soils with low water-holding capacity. GPP of larch communities showed an increasing trend, whereas NPP stagnated. A similar pattern of GI response to climate warming has also been observed for Larix sibirica Ledeb, Pinus sibirica Du Tour, and Abies sibirica Ledeb in the forests of southern Siberia. Thus, warming in northern Siberia permafrost zone resulted in an initial increase in larch growth from the 1970s to the mid-1990s. After that time, larch growth increment has decreased. Since ca. 1990, water stress at the beginning of the vegetative period became, along with air temperature, a main factor affecting larch growth within the permafrost zone.

Larix dahurica↗

A re-analysis of the spectrum of 2206-199

Recently Pettini, Hunstead, Smith and Mar, (PHSM 1991) performed an analysis of the Ly-alpha forest of QSO 2206-199N at very high resolution. On the basis of their observations they concluded that most Doppler parameters b = (square root of 2)(sigma) of Ly-alpha forest lines are below 22 km/s, with a range down to a few km/s and a median of 17 km/s. They also found a strong intrinsic correlation between Doppler parameter b and column density N. These results are in contrast to those of a similar study by Carswell, Lanzetta, Parnell, and Webb (CLPW 1991) at comparable resolution with the same instruments, who find that most of the Ly-alpha lines towards QSO 1100-264 have Doppler parameters above 15 km/s (median b = 34 km/s), and that there is no significant correlation between b and N. Whilst an intrinsic difference between the lines of sight to 2206-199 and to other QSO's can not be excluded a priori, previous disagreement between Doppler parameter estimates obtained by both groups pointed to a potential difference in estimation techniques and in the interpretation of the results. To investigate this possibility, the AAT/UCLES spectrum of 2206-199 obtained by PHSM were reanalyzed. The spectrum was extracted from the raw data and determined the line parameters using the method described by CLPW.

Rauch, M.↗

Well-posedness of one-way wave equations and absorbing boundary conditions

A one-way wave equation is a partial differential which, in some approximate sense, behaves like the wave equation in one direction but permits no propagation in the opposite one. The construction of such equations can be reduced to the approximation of the square root of (1-s sup 2) on -1, 1 by a rational function r(s) = p sub m (s)/q sub n(s). Those rational functions r for which the corresponding one-way wave equation is well-posed are characterized both as a partial differential equation and as an absorbing boundary condition for the wave equation. We find that if r(s) interpolates the square root of (1-s sup 2) at sufficiently many points in (-1,1), then well-posedness is assured. It follows that absorbing boundary conditions based on Pade approximation are well-posed if and only if (m, n) lies in one of two distinct diagonals in the Pade table, the two proposed by Engquist and Majda. Analogous results also hold for one-way wave equations derived from Chebyshev or least-squares approximation.

Trefethen, L. N.↗

2017 Meander C sediment characterization from the East River, Colorado

This dataset includes characterization data collected on sediment samples from Meander C as part of the Watershed Function Scientific Focus Area (SFA) located in the Upper Colorado River Basin. The data were collected to investigate the nature of mineral-organic associations across the meander transect and with depth. Samples were collected in 2017, and analyzed using sequential extractions (water, pyrophosphate, HCl, and dithionite) and density fractionation. Data collected include: texture, pH, root biomass, and metals (Fe, Al, Ca, Si) of bulk sediment samples; C and N of heavy fraction; and extractable OC, Fe, and Al, and organic matter characterization using high-resolution mass spectrometry (FT-ICR-MS) of sequential extractions. Sample locations are included in the ‘sample_metadata.xlsx” file. Sediment characterization data is included in the accompanying csv files: bulk and density fractions (MCB2017_density-and-bulk-fractions.csv), sequential extractions (MCB2017_sequential-extractions.csv), and FT-ICR-MS data from sequential extractions in three files to be read by the R package ftmsRanalysis (MCB2017_FTICRMS-sequential-edata.csv, MCB2017_FTICRMS-sequential-emeta.csv, and MCB2017_FTICRMS-sequential-fdata.csv). Sediment was collected in triplicate; ID’s refer to individual samples.

54 ENVIRONMENTAL SCIENCES↗

Two-loop MHV form factors from the periodic Wilson loop

We discuss how to compute maximal-helicity-violating (MHV) form factors for the chiral part of the stress-tensor supermultiplet from periodic light-like polygon Wilson loops in planar $\mathcal{N}$ = 4 super Yang-Mills theory beyond the one-loop level. We show that the periodicity imposes path ordering on points on different edges, which explains the appearance of square roots coming from non-planar Feynman diagrams. Taking such diagrams into account, we provide the integrand of the two-loop n-particle MHV form factor, compute all diagrams, prove the cancellation of divergences and finally compute the two-loop 5-particle and 6-particle form factors as examples.

72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS↗

Ferredoxin and ferredoxin-NADP reductase from photosynthetic and nonphotosynthetic tissues of tomato

Ferredoxin and ferredoxin-NADP+ oxidoreductase (FNR) were purified from leaves, roots, and red and green pericarp of tomato (Lycopersicon esculentum, cv VFNT and cv Momotaro). Four different ferredoxins were identified on the basis of N-terminal amino acid sequence and charge. Ferredoxins I and II were the most prevalent forms in leaves and green pericarp, and ferredoxin III was the most prevalent in roots. Red pericarp of the VFNT cv yielded variable amounts of ferredoxins II and III plus a unique form, ferredoxin IV. Red pericarp of the Momotaro cv contained ferredoxins I, II, and IV. This represents the first demonstration of ferredoxin in a chromoplast-containing tissue. There were no major differences among the tomato ferredoxins in absorption spectrum or cytochrome c reduction activity. Two forms of FNR were present in tomato as judged by anion exchange chromatography and by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. FNR II had a lower apparent relative molecular weight, a slightly altered absorption spectrum, and a lower specific activity for cytochrome c reduction than FNR I. FNR II could be a partially degraded form of FNR I. The FNRs from the different tissues of tomato plants all showed diaphorase activity, with FNR II being more active than FNR I. The presence of ferredoxin and FNR in heterotrophic tissues of tomato is consistent with the existence of a nonphotosynthetic ferredoxin/FNR redox pathway to support the function of ferredoxin-dependent enzymes.

NASA Discipline Number 52-30↗

CODEX: Role of velocity substructure in the scaling relations of galaxy clusters

The use of galaxy clusters as cosmological probes relies on a detailed understanding of their properties. They define cluster selection and ranking linked to a cosmologically significant cluster mass function. Previous studies have employed small samples of clusters, concentrating on achieving the first calibrations of cluster properties with mass, while the diversity of cluster properties has been revealed via detailed studies. The large spectroscopic follow-up on the CODEX cluster sample with SDSS and NOT enables a detailed study of hundreds of clusters, lifting the limitations of previous samples. We aim to update the spectroscopic cluster identification of CODEX by running the spectroscopic group finder on the follow-up spectroscopy results and connecting the dynamical state of clusters to their scaling relations. We implemented a reproducible spectroscopic membership determination and cleaning procedures, based on the redMaPPer membership, running the spectroscopic group finder on the follow-up spectroscopy results and cleaning the membership for spectroscopic outliers. We applied the Anderson-Darling test for velocity substructure and analysed its influence on the scaling relations. We also tested the effect of the X-ray-to-optical centre offset on the scaling relations. We report on the scaling relations between richness, X-ray luminosity, and velocity dispersion for a complete sample of clusters with at least 15 members. Clusters with velocity substructure exhibit enhanced velocity dispersion for a given richness and are characterized by 2.5 times larger scatter. Clusters that have a strong offset in X-ray-to-optical centres have comparable scaling relations as clusters with substructure. We demonstrate that there is a consistency in the parameters of the scaling relations for the low- and high-richness galaxy clusters. Splitting the clusters by redshift, we note a decrease in scatter with redshift in all scaling relations. We localize the redshift range where a high scatter is observed to z < 0.15, which is in agreement with the literature results on the scatter. We note that the increase in scatter for both high- and low-luminosity clusters is z < 0.15, suggesting that both cooling and the resulting active galactic nucleus feedback are at the root of this scatter.

79 ASTRONOMY AND ASTROPHYSICS↗

Patterns of Element Incorporation in Calcium Carbonate Biominerals Recapitulate Phylogeny for a Diverse Range of Marine Calcifiers

Elemental ratios in biogenic marine calcium carbonates are widely used in geobiology, environmental science, and paleoenvironmental reconstructions. It is generally accepted that the elemental abundance of biogenic marine carbonates reflects a combination of the abundance of that ion in seawater, the physical properties of seawater, the mineralogy of the biomineral, and the pathways and mechanisms of biomineralization. Here we report measurements of a suite of nine elemental ratios (Li/Ca, B/Ca, Na/Ca, Mg/Ca, Zn/Ca, Sr/Ca, Cd/Ca, Ba/Ca, and U/Ca) in 18 species of benthic marine invertebrates spanning a range of biogenic carbonate polymorph mineralogies (low-Mg calcite, high-Mg calcite, aragonite, mixed mineralogy) and of phyla (including Mollusca, Echinodermata, Arthropoda, Annelida, Cnidaria, Chlorophyta, and Rhodophyta) cultured at a single temperature (25°C) and a range of p CO 2 treatments (ca. 409, 606, 903, and 2856 ppm). This dataset was used to explore various controls over elemental partitioning in biogenic marine carbonates, including species-level and biomineralization-pathway-level controls, the influence of internal pH regulation compared to external pH changes, and biocalcification responses to changes in seawater carbonate chemistry. The dataset also enables exploration of broad scale phylogenetic patterns of elemental partitioning across calcifying species, exhibiting high phylogenetic signals estimated from both uni- and multivariate analyses of the elemental ratio data (univariate: λ = 0–0.889; multivariate: λ = 0.895–0.99). Comparing partial R 2 values returned from non-phylogenetic and phylogenetic regression analyses echo the importance of and show that phylogeny explains the elemental ratio data 1.4–59 times better than mineralogy in five out of nine of the elements analyzed. Therefore, the strong associations between biomineral elemental chemistry and species relatedness suggests mechanistic controls over element incorporation rooted in the evolution of biomineralization mechanisms.

58 GEOSCIENCES↗

Influence of shrub willow buffers strategically integrated in an Illinois corn-soybean field on soil health and microbial community composition

We report soil serves many important ecological functions and is an integral part of our existence as a society. However, concerns for soil health are growing globally, in part due to the negative impacts of agricultural management on soil resources. The production of perennial bioenergy crops on marginal land in row-crop production systems is one solution that could improve land-use efficiency and address the sustainability of cropland management. Because the relationship between crop management and the environment is complex, more research is needed to evaluate the potential benefits perennial bioenergy crop production has on soil health, as well as other ecosystem services. In this study, shrub willow buffers were strategically integrated into a corn-soybean cropping system with the main objective of reducing nitrate-N leaching from grain crop production while producing biomass for bioenergy. Two buffer systems (defined by landscape positions) were included for comparison, one on marginal land with exposure to nitrate-N leaching from upslope grain (southern plots) and one on fertile soils with less nitrate-N leaching potential (northern plots). Evaluation of soil (chemistry, bulk density, microbial community) and shrub willow vegetation properties ( fine roots, leaf litter decomposition, and nutrient uptake dynamics), showed that landscape position plays an important role in (1) the dynamics of soil chemical properties, (2) shrub willow's influence and productivity, and (3) the provision of additional ecosystem services such as reductions in nitrous oxide emissions and nitrate-N leaching. In addition, the combination of crop type and landscape position (N-grain, N-willow, S-grain, and S-willow) influenced the species composition of the soil microbial community, resulting in unique and identifiable communities. These results highlight the potential application of shrub willow buffers for ecosystem service provision and support of ecosystem processes; however, understanding the relationship between the microbial community, crop type, and landscape is important for understanding the sustainability of the design.

54 ENVIRONMENTAL SCIENCES↗

Numerical challenges for energy conservation in N -body simulations of collapsing self-interacting dark matter halos

Dark matter (DM) halos can be subject to gravothermal collapse if the DM is not collisionless, but engaged in strong self-interactions instead. When the scattering is able to efficiently transfer heat from the centre to the outskirts, the central region of the halo collapses and reaches densities much higher than those for collisionless DM. This phenomenon is potentially observable in studies of strong lensing. Current theoretical efforts are motivated by observations of surprisingly dense substructures. However, a comparison with observations requires accurate predictions. One method to obtain such predictions is to use N-body simulations. Collapsed halos are extreme systems that pose severe challenges when applying state-of-the-art codes to model self-interacting dark matter (SIDM). In this work, we investigate the root of such problems, with a focus on energy non-conservation. Moreover, we discuss possible strategies to avoid them. We ran N-body simulations, both with and without SIDM, of an isolated DM-only halo and we adjusted the numerical parameters to check the accuracy of the simulation. We find that not only the numerical scheme for SIDM can lead to energy non-conservation, but also the modelling of gravitational interaction and the time integration are problematic. The main issues we find are: (a) particles changing their time step in a non-time-reversible manner; (b) the asymmetry in the tree-based gravitational force evaluation; and (c) SIDM velocity kicks breaking the time symmetry. Tuning the parameters of the simulation to achieve a high level of accuracy allows us to conserve energy not only at early stages of the evolution, but also later on. However, the cost of the simulations becomes prohibitively large as a result. Some of the problems that make the simulations of the gravothermal collapse phase inaccurate can be overcome by choosing appropriate numerical schemes. However, other issues still pose a challenge. Our findings motivate further works on addressing the challenges in simulating strong DM self-interactions.

dark matter↗

Root exudate composition from different plant species influences the growth of rhizosphere bacteria

Plant roots release exudates that fuel microbial activities and can structure rhizosphere microbial communities, but how different plant species use their root exudate to potentially select for different soil microbes in the rhizosphere is not well understood. Here, we investigated how root exudate from plants of three diverging lineages, Lactuca sativa (lettuce), Brassica juncea (mustard cabbage), and Zea mays (maize) influence the growth of their own rhizosphere bacteria (host) and those from other plant species (non-host) in growth bioassays. We found that on the community level, lettuce rhizosphere bacteria grew better in non-host exudate, but mustard cabbage and maize rhizosphere bacteria grew similarly well in both host and non-host exudate. However, individual bacteria taxa showed strong preferences for exudate from different plant species. The bacterial growth patterns were independent of C and N quantity, suggesting that certain exometabolic compounds may drive the growth patterns. Our results demonstrate that root exudate from a given plant species have the potential to stimulate or suppress soil bacteria and hint at a mechanism that different plant species use to select for their specific suite of rhizosphere bacteria. Furthermore, these findings contribute to our broader understanding of how root exudate quality could be a mechanism that plants use to select for distinct microbial communities in the rhizosphere.

54 ENVIRONMENTAL SCIENCES↗

Reduced naphthylphthalamic acid binding in the tir3 mutant of Arabidopsis is associated with a reduction in polar auxin transport and diverse morphological defects

Polar auxin transport plays a key role in the regulation of plant growth and development. To identify genes involved in this process, we have developed a genetic procedure to screen for mutants of Arabidopsis that are altered in their response to auxin transport inhibitors. We recovered a total of 16 independent mutants that defined seven genes, called TRANSPORT INHIBITOR RESPONSE (TIR) genes. Recessive mutations in one of these genes, TIR3, result in altered responses to transport inhibitors, a reduction in polar auxin transport, and a variety of morphological defects that can be ascribed to changes in indole-3-acetic acid distribution. Most dramatically, tir3 seedlings are strongly deficient in lateral root production, a process that is known to depend on polar auxin transport from the shoot into the root. In addition, tir3 plants display a reduction in apical dominance as well as decreased elongation of siliques, pedicels, roots, and the inflorescence. Biochemical studies indicate that tir3 plants have a reduced number of N-1-naphthylphthalamic (NPA) binding sites, suggesting that the TIR3 gene is required for expression, localization, or stabilization of the NPA binding protein (NBP). Alternatively, the TIR3 gene may encode the NBP. Because the tir3 mutants have a substantial defect in NPA binding, their phenotype provides genetic evidence for a role for the NBP in plant growth and development.

Non-NASA Center↗

Mass production of high-performance single atomic FeNC electrocatalysts via sequenced ultrasonic atomization and pyrolysis process

Mass production of highly efficient, durable, and inexpensive single atomic catalysts is currently the major challenge associated with the oxygen reduction reaction (ORR) for fuel cells. In this study, we develop a general strategy that uses a simple ultrasonic atomization coupling with pyrolysis and calcination process to synthesize single atomic FeNC catalysts (FeNC SACs) at large scale. The microstructure characterizations confirm that the active centers root in the single atomic Fe sites chelating to the four-fold pyridinic N atoms. The identified specific Fe active sites with the variable valence states facilitate the transfer of electrons, endowing the FeNC SACs with excellent electrochemical ORR activity. The FeNC SACs were used as cathode catalysts in a homemade Zn-air battery, giving an open-circuit voltage (OCV) of 1.43 V, which is substantially higher than that of commercial Pt/C catalysts. This study provides a simple approach to the synthesis of single atomic catalysts at large scale.

36 MATERIALS SCIENCE↗

Tomato responses to ammonium and nitrate nutrition under controlled root-zone pH

Tomato (Lycopersicon esculentum L. Mill. 'Vendor') plants were grown for 21 days in flowing solution culture with N supplied as either 1.0 mM NO3- or 1.0 mM NH4+. Acidity in the solutions was automatically maintained at pH 6.0. Accumulation and distribution of dry matter and total N and net photosynthetic rate were not affected by source of N. Thus, when rhizosphere acidity was controlled at pH 6.0 during uptake, either NO3- or NH4+ can be used efficiently by tomato. Uptake of K+ and Ca2+ were not altered by N source, but uptake of Mg2+ was reduced in NH4(+)-fed plants. This indicates that uptake of Mg2+ was regulated at least partially by ionic balance within the plant.

NASA Program CELSS↗

Abscisic acid, xanthoxin and violaxanthin in the caps of gravistimulated maize roots

The occurrence and distribution of abscisic acid (ABA), xanthoxin (Xa) and the carotenoid violaxanthin (Va) were investigated in root tips of maize (Zea mays L. cv. Merit). In roots grown in the dark, Va and ABA were present in relatively high amounts in the root cap and in low amounts in the adjacent terminal 1.5 mm of the root. Xanthoxin was present in equal concentrations in both regions. In roots exposed to light, the ABA distribution was reversed, with relatively low levels in the root cap and high levels in the adjacent 1.5-mm segment. Light also caused a decrease in Va in both regions of the root and an increase in Xa, especially in the cap. In the maize cultivar used for this work, light is necessary for gravitropic curving. This response occurs within the same time frame as the light-induced ABA redistribution as well as the changes in the levels of Va and Xa. These data are consistent with a role for ABA in root gravitropism and support the proposal that Xa may arise from the turnover of Va.

NASA Discipline Plant Biology↗