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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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At least 217 records · Page 12
All sourcesNASA NTRSDOE OSTI
NASA NTRS1988

Temperature response of Antarctic cryptoendolithic photosynthetic microorganisms

Growth responses to temperatures between 12.5 [degrees] C and 25 degrees C were determined for five photosynthetic microorganisms isolated from the Ross Desert cryptoendolithic community. Among eukaryotic algae, two strains of Trebouxia sp. have an upper temperature limit of 20 degrees C, and two strains of Hemichloris antarctica of 25 degrees C. The cyanobacterium Chroococcidiopsis sp., in contrast, grows at temperatures above 25 degrees C. These and earlier studies suggest that the eukaryotic algae of the Antarctic cryptoendolithic community have an upper temperature limit near 25 degrees C.

NASA Discipline Exobiology↗
NASA NTRS2006

Novel Concept for LSS Based on Advanced Microalgal Biotechnologies

One of the key issues for successful human space exploration is biomedical life support in hostile space and planetary environments that otherwise cannot sustain life. Bioregenerative life support systems (LSS) are one of the options for atmospheric regeneration. To date, no bioregenerative LSS has shown capability for 100% air regeneration. Nor have these LSS been robust enough to simultaneously provide a regenerable complete food source. In contrast to microalgae, traditional plant approaches, e.g. wheat and lettuce, are lacking essential amino acids, vitamins, and micronutrients. Moreover, the rate of photosynthesis by microalgae significantly exceeds that of high plants. Nevertheless, the employment of microalgae in LSS technology was restricted, until recently, due to high water demands. Also the per person requirement of a 40L volume of microalgae in a photobioreactor, to provide daily O2 production, made an algae-based approach less attractive. By employing a vertically stacked membrane bioreactor, coupled with a solar tracker and photon-delivery system, a lightweight air revitalization system for space based applications, with minimal water requirements, can be developed. Our preliminary estimations suggest that a membrane bioreactor, 8m3 in volume, comprised of 80m2 (twenty 2m x 2m membranes, each spaced 10 cm apart), and a total of 70L of water could produce 2.7 kg of dried microalgal biomass that would supply the energy and essential amino acid requirements, as well as producing sufficient O2 for the daily needs of a 15 member crew. Research on the biochemical content of edible blue-green alga Spirulina (Arthrospira) platensis shows a wide spectrum of stable Spirulina mutants with an enhanced content of amino acids, -carotene, and phycobilliprotein c-phycocyanin. Feeding animals suffering from radiation-induced lesions, c-phycocyanin, extracted from strain 27G, led to a correction in the decrement of dehydrogenase activity and energy-rich phosphate levels, as well as improved antioxidant defense and pyruvate levels, compared to untreated animals. Experimental anemia in rats was corrected by feeding Spirulina platensis strains 198B and 27G, (with an enhanced content of methionine, phycobiliproteins and carotenoids). Spirulina was recently shown by Ananyev et al, 2005, to be an oxygenic organism with the highest level of photosystem II activity (O2 production). We propose therefore to develop a design for membrane-based photoreactors for Lunar and Mars exploration habitat LSS, for the cultivation of genetically modified strains of Spirulina to scrub CO2 and supply astronauts with O2, protein, vitamins, and immunostimulators.

Brown, I.↗
NASA NTRS2010

OMEGA for the Future of Biofuels

OMEGA: Offshore Membrane Enclosure for Growing Algae. To develop a photobioreactor (PBR) for growing algae (Oil, food, fertilizer) that does not compete with agriculture for land (deployed offshore), water or fertilizer (uses/treats wastewater).

Trent, Jonathan↗
NASA NTRS2016

Caribbean Oceans: Utilizing NASA Earth Observations to Detect, Monitor, and Respond to Unprecedented Levels of Sargassum in the Caribbean Sea

In 2011 and 2015, the nations of the Caribbean Sea were overwhelmed by the unprecedented quantity of Sargassum that washed ashore. This issue prompted international discussion to better understand the origins, distribution, and movement of Sargassum, a free-floating brown macro alga with ecological, environmental, and commercial importance. In the open ocean, Sargassum mats serve a vital ecological function. However, when large quantities appear onshore without warning, Sargassum threatens local tourist industries and nearshore ecosystems within the Caribbean. As part of the international response, this project investigated the proliferation of this macro alga within the Caribbean Sea from 2003-2015, and used NASA Earth observations to detect and model Sargassum growth across the region. The Caribbean Oceans team calculated the Floating Algal Index (FAI) using Terra Moderate Resolution Imaging Spectroradiometer (MODIS) data, and compared the FAI to various oceanic variables to determine the ideal pelagic environment for Sargassum growth. The project also examined the annual spread of Sargassum throughout the region by using Earth Trends Modeler (ETM) in Clark Labs' TerrSet software. As part of the international effort to better understand the life cycle of Sargassum in the Caribbean, the results of this project will help local economies promote sustainable management practices in the region.

Ped, Jordan↗
NASA NTRS2019

Applicability of Spectral Decomposition by Varimax-Rotated, Principal Component Analysis to the Surface Biology and Geology (SBG) VNIR Mission Concept

Cyanobacterial and Harmful Algal Blooms (CyanoHABs) are a growing concern in coastal and inland waters. But, spectral interference from multiple constituents in optically complex waters can hamper application of remote sensing using traditional image processing methods. The Kent State University (KSU) spectral decomposition method can be applied to multispectral and hyperspectral remote sensing images (e.g. HICO and the NASA Glenn HSI2) to partition and identify signals related to cyanobacteria, algae, pigment degradation products and suspended sediment in each pixel. Fundamental to the use of remote sensing data is the ability to extract independent signals from correlated hyperspectral VNIR data cubes. The Kent State University varimax-rotated, principal component analysis method (VPCA) is important to integrate into the SBG VNIR mission concept because it provides greater specificity, a software-based SNR boost relative to hardware performance, and can assist with Cal/Val, Modeling and Applications. We present examples of the hyperspectral application of the KSU VPCA method with relevance to SBG. The information extracted by VPCA can be validated spectrally or spatially with laboratory and/or in situ sensors, which capture spatial or time series of information at discrete points within remote sensing images. Comparisons show hyperspectral sensors extract more components than multispectral ones, but more independent information can be extracted from multispectral sensors by VPCA than traditional band ratio approaches. The spectral decomposition method is capable of enhancing the signal to noise ratio (SNR) of the NASA Glenn, second-generation hyperspectral imager by a factor of 7x to 20x, with a spectral reproducibility of ±3%. The spectral decomposition method, when compared against existing remote sensing monitoring methods exhibits both greater specificity and a lower detection limit. The method has been validated with multispectral images in Lake Erie to quantify the Microcystis CyanoHAB and from the Indian River Lagoon, Florida to quantify the Brown Tide resulting from A. lagunesnsis. Field operations in the Western Basin of Lake Erie were conducted using a bbe Fluoroprobe to collect vertical profiles and horizontal tows along a transect from the Toledo to the Detroit Lighthouse during coincident satellite overpasses. Extraction of pixel values from the MODIS Aqua sensor yields agreement between in situ field and lab-based measures of cyanobacterial, cryptophyte, diatoms and green algae, suspended sediment and pigment degradation products with R2>0.8.

hyperspectral↗
NASA NTRSDate not supplied

Determining Chlamydomonas reinhardtii resistance to ionizing radiation at a genome-wide scale

Spaceflight is a complex mixture of abiotic stresses that causes a myriad of cellular responses including increased genome-wide mutation load. The impact of spaceflight on mutation load is not well understood for most organisms and is especially critical to characterize for candidate microorganisms that could be part of biological life support systems. Prior research in our laboratory revealed an unexpectedly high level of mutation during spaceflight in the eukaryotic green algae, C. reinhardtii. Based on the spectrum of base changes observed, we concluded that the mutations were generated through a molecular mechanism unique to the spaceflight environment. Spaceflight is a complex set of stresses, and we are using an Earth-based analog to test ionizing radiation as a potential causative variable for the unique mutation spectrum observed in spaceflight. C. reinhardtii was grown exposed to chronic radiation from a Cesium-137 source. Four biological replicate cultures of wild-type cells were grown in gas permeable plastic tissue culture bags in liquid media following the design used on the International Space Station (ISS) during the previous space algae experiment. Radiation levels for this experiment approximate ionizing radiation observed on the moon, in low Earth orbit (LEO), and on Earth. Cells from each replicate were passaged to inoculate the next cycle of growth, then samples were taken from each culture to characterize cell morphology, quantify biomass, and extract DNA. Whole genome sequencing from each culture will be used to identify the frequency and types of mutations in each radiation level. In growth cycles 3 and 4, there was a significant increase in biomass accumulation under lunar radiation level compared to LEO or Earth radiation levels. These results such that ionizing radiation affected growth of C. reinhardtii.

Microbiology↗
NASA NTRS2022

Discovery of Chlorophyll d : Isolation and Characterization of a Far-Red Cyanobacterium from the Original Site of Manning and Strain (1943) at Moss Beach, California

We have isolated a chlorophyll-d-containing cyanobacterium from the intertidal field site at Moss Beach, on the coast of Central California, USA, where Manning and Strain (1943) originally discovered this far-red chlorophyll. Here, we present the cyanobacterium’s environmental description, culturing procedure, pigment composition, ultrastructure, and full genome sequence. Among cultures of far-red cyanobacteria obtained from red algae from the same site, this strain was an epiphyte on a brown macroalgae. Its Q y in vivo absorbance peak is centered at 704–705 nm, the shortest wavelength observed thus far among the various known Acaryochloris strains. Its Chl a /Chl d ratio was 0.01, with Chl d accounting for 99% of the total Chl d and Chl a mass. TEM imagery indicates the absence of phycobilisomes, corroborated by both pigment spectra and genome analysis. The Moss Beach strain codes for only a single set of genes for producing allophycocyanin. Genomic sequencing yielded a 7.25 Mbp circular chromosome and 10 circular plasmids ranging from 16 kbp to 394 kbp. We have determined that this strain shares high similarity with strain S15, an epiphyte of red algae, while its distinct gene complement and ecological niche suggest that this strain could be the closest known relative to the original Chl d source of Manning and Strain (1943). The Moss Beach strain is designated Acaryochloris sp. (marina) strain Moss Beach.

chlorophyll d↗
NASA NTRSDate not supplied

CyanoSCape: Freshwater Phytoplankton and Floating Aquatic Vegetation Biodiversity

In Southern Africa, the impacts of anthropogenic activities on biodiversity and ecosystem services are exacerbated by the climate crisis. Rapid land use change and the lack of emphasis on environmentally sustainable agricultural practices has hindered hydrological processes and compromised riverine and aquatic ecosystems. This poses obvious risks to natural/indigenous aquatic biodiversity and long-term ecosystem sustainability. Phytoplankton serve as the foundation of the freshwater food web with zooplankton as consumers, which feed fish, invertebrates, and so on up the food chain that comprises the biodiversity of the freshwater system that serves as habitat for biodiversity as well. The diversity of phytoplankton (microscopic organisms) includes photosynthesizing bacteria (cyanobacteria), plant-like diatoms, dinoflagellates, and green algae. Nutrient run-off from agricultural fertilizers and urban overflows, warm temperatures, abundant light availability and compromised hydrological systems provide an ideal environment for cyanobacteria to flourish. Increased prevalence of cyanobacteria, due to both natural and anthropogenic causes, can incur significant effects on the biodiversity of the overall phytoplankton assemblage. These bloom forming algae can significantly outcompete other phytoplankton classes in warmer and eutrophic waters where they are quick to dominate the freshwater system. Eutrophication and toxic cyanobacteria blooms (cyanoHABs) in the inland waters of the Greater Cape Floristic Region (GCFR) incur significant effects on the biodiversity of the overall phytoplankton assemblage and provide a favorable environment forthe overgrowth of floating aquatic vegetation (FAV), which is often invasive and associated with reduced aquatic biodiversity. The algal biodiversity of the GCFR’s freshwater systems is not well characterized. Hyperspectral optical observations are expected to facilitate the improvement of current phytoplankton functional type retrievals significantly, as the sensitivity is sufficient that the distinctive, fine spectral features of different phytoplankton groups can be detected. This will enable testing emerging algorithms and inform the development of new algorithms for use with upcoming hyperspectral satellite missions in this decade. Innovations in optical sensor sensitivity and next generation machine learning capabilities considerably enhance the potential for accurate and rapid detection of phytoplankton, namely the presence, extent, and diversity of cyanobacteria present in cyanoHABs and additionally, invasive FAV. Upcoming hyperspectral satellite missions such as NASA’s Surface Biology and Geology (SBG), Plankton, Aerosol, Cloud, ocean Ecosystem (PACE), and the European Space Agency’s Copernicus Hyperspectral Imaging Mission (CHIME) will provide imagery with unprecedented spectral and spatial resolution which will further enable the discovery of linkages between the seasonality and dynamics of HABs and FAV. The overarching goal of this project is to utilize hyperspectral data, with recently developed and next-generation algorithms, to determine the biodiversity of freshwater systems phytoplankton assemblage with emphasis on genus level distinction, as well as monitor the prevalence and diversity of FAV.

CyanoSCape↗
NASA NTRSDate not supplied

Determining Chlamydomonas Reinhardtii Resistance to Ionizing Radiation at A Genome-Wide Scale

Spaceflight is a complex mixture of abiotic stresses that causes a myriad of cellular responses including increased genome-wide mutation load. The impact of spaceflight on mutation load is not well understood for most organisms and is especially critical to characterize for candidate microorganisms that could be part of biological life support systems. Prior research in our laboratory revealed an unexpectedly high level of mutation during spaceflight in the eukaryotic green algae, C. reinhardtii. Based on the spectrum of base changes observed, we concluded that the mutations were generated through a molecular mechanism unique to the spaceflight environment. Spaceflight is a complex set of stresses, and we are using an Earth-based analog to test ionizing radiation as a potential causative variable for the unique mutation spectrum observed in spaceflight. C. reinhardtii was grown exposed to chronic radiation from a Cesium-137 source. Four biological replicate cultures of wild-type cells were grown in gas permeable plastic tissue culture bags in liquid media following the design used on the International Space Station (ISS) during the previous space algae experiment. Radiation levels for this experiment approximate ionizing radiation observed on the moon, in low Earth orbit (LEO), and on Earth. Cells from each replicate were passaged to inoculate the next cycle of growth, then samples were taken from each culture to characterize cell morphology, quantify biomass, and extract DNA. Whole genome sequencing from each culture will be used to identify the frequency and types of mutations in each radiation level. In growth cycles 3 and 4, there was a significant increase in biomass accumulation under lunar radiation level compared to LEO or Earth radiation levels. These results show that ionizing radiation affected growth of C. reinhardtii.

Hope Hersh↗
NASA NTRSDate not supplied

Unleashing the Power of Anaerobic–Phototrophic Membrane Bioreactors for Sustainable Bioregenerative Life Support

Using the core ideals of bioregenerative life support, an anaerobic–phototrophic membrane bioreactor (APMBR) has been designed and operated at NASA’s Kennedy Space Center to treat complex wastewaters with the goal of closing water and nutrient cycles on early planetary bases. The system combined the previously operated anaerobic membrane bioreactor and the phototrophic membrane bioreactor that have been detailed in presentations at previous ICES conferences. This newly combined system is able to treat complex wastewater with completely automated controls on a small footprint. The treatment of wastes in this APMBR is as follows: (1) the waste enters the anaerobic subsystem, where solids are hydrolyzed and carbon is removed via anaerobic digestion, (2) an ultrafiltration membrane is used to separate the solids and the recovered water, (3) the effluent from the anaerobic subsystem is fed to the phototrophic subsystem on command, (4) the algae–bacteria consortium aids in nitrification in the recovered water, and (5) an ultrafiltration membrane is used to separate the algae and the final recovered water. The recovered water from the APMBR is rich in nutrients, making it a sustainable source of fertilizer for downstream hydroponic systems. This conference paper will detail the design and operation of the APMBR as a bioregenerative alternative to physical–chemical systems or bag and storage systems. In this paper, data will be presented on subsystem water quality, membrane performance, and effluent quality. Overall, the APMBR has the ability to treat wastewater using a combination of biological and filtration technologies that allow for higher removal efficiencies, low consumable use, and small footprint.

Jason A. Fischer↗
NASA NTRSDate not supplied

Identifying Indicators of Harmful Algal Blooms in Coastal Atacama Using Satellite Image Processing Techniques to Improve Industry and Authority Response

Over the past decade, Chile has experienced a significant rise in both the frequency and intensity of coastal Harmful Algal Blooms (HABs). These bloom events are a growing concern for the Atacama Region especially, with potential impacts on human health, aquaculture, and the environment. HABs are caused by an excess proliferation of microalgae, with certain algae species commonly found in Chilean HABs capable of producing toxins which can poison fish, toxify shellfish, and cause illness or death when ingested by humans. Collaborating with the Ministry of Health of Chile, Centro de Información de Recursos Naturales, the University of Atacama, and the Embassy of Chile’s Agricultural Office, this study identifies potential indicators of HABs in the Coastal Atacama Region using NASA Earth observations. Satellite imagery from the Moderate Resolution Imaging Spectroradiometer (MODIS) instrument onboard NASA’s Aqua satellite was used for the identification of chlorophyll-a which provides direct estimates of the algae pigment’s concentration in water bodies. In addition, the spatiotemporal patterns of several other parameters were examined to identify correlations with occurrences of HABs, including sea surface temperature and normalized fluorescence line height using Aqua MODIS, Normalized Difference Turbidity Index (NDTI) using Landsat 8, and ocean wind speed measurements from the NOAA Joint Polar Satellite System. Analysis of these patterns revealed hotspots of high chlorophyll-a concentrations from the years 2014 to 2022, the most major of which occurring in the coastal zones of Chañaral, Bahía Inglesa, and Huasco. Also, through the use of a similarity search conducted, the seasonal variation of these indicators was mapped to highlight areas with great likelihood of experiencing HABs in the future. The results can guide future efforts for in-situ water sampling, toxin analysis and assessment, and HAB monitoring and prevention.

Cody O'Ferrall↗
NASA NTRS1962

LIFE IN EXTRATERRESTRIAL ENVIRONMENTS

The objective of this program was to establish whether terrestrial life forms can survive and thrive in extraterrestrial environments. Other than Earth, the only solar body capable of supporting terrestrial life appears to be the planet Mars. The conditions selected to simulate a Martian environment were determined by a literature search and were: 1) Light of > 4800-A wavelength 2) Temperature changes from a diurnal high of 30 °C to a nocturnal low to -60 °C 3) Atmosphere composed of 95% nitrogen, 4% argon, 0.3% carbon dioxide, and less than 1% oxygen and moisture. The organisms tested in thesimjulated Martian environment were: 1) Algae: Trebouxia erici 2) Bacteria: Clostridium tetani, Bacillus subtilis, and Escherichia coli 3) Bryophytes: the liverwort Marchantia polymorpha; the mosses Eurhynchium strigosum, Atrichum undulatum, Mnium sp., Brium sp., Grimmia sp., Pogonatum sp., and Polytrichum sp. 4) Lichens: Cladonia conoeraea, C. rangifernia, C. cristatella, C. pyxidate, Parmelia conspersa var. isidata, P. conspersa var. leucophora, Lecidea cyanea, Umbilicaria dillenii, Physcia hypoleuca, and Ramalina sp. T. erici survived for about 2 weeks. C. tetani and E. coli cells decreased rapidly in number, but the tetanus toxin remained active for the 127=day period of the experiment. B. subtilis cells increased in number. Of the Bryophytes, only one moss survived. Complete results for the lichens are not yet available. It is suggested that the observed color changes on the Martian surface may be explained in part by color changes in the surface vegetation as a function of moisture content.

Mars (Planet)↗