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At least 217 records · Page 12

Modeling Exhaust-Generated Aerodynamic Pressure Loads on Airfield Matting Repair Systems

Airfield matting systems are commonly used for rapid repair of damaged runways to facilitate continuity of critical operations. Under normal service conditions, matting repair systems are subject not only to wheel loads exerted by airfield traffic but also to aerodynamic pressure loads resulting from high-speed, turbulent exhaust plumes produced by jets during taxi and take-off. Matting systems employed by the U.S. Air Force have been tested with respect to wheel loads, but probabilities of failure because of pressure loads produced by jet exhaust have not yet been established. This study presents a numerical approach for preliminary estimation of worst-case matting anchor forces resulting from exhaust-generated pressure loads. Using a two-dimensional computational fluid dynamics model, system behavior is evaluated by means of a parametric study of six system variables, of which the most significant are: (1) distance between engine and matting, (2) depth of cavity openings at matting edges, and (3) engine exhaust velocity. The results demonstrate that matting systems are likely to experience net uplift in typical service scenarios, driven by the combined effects of flow separation and cavity pressurization. Worst-case anchor pull-out forces, computed according to a tributary-area approach, are estimated to fall in the range of 130 lb (581 N) to 979 lb (4,350 N), depending on assumed load-sharing behavior among anchors and the size of the repair site. Field testing of instrumented matting systems during jet taxi and take-off sequences is recommended as the best next step toward understanding system behavior.

Engineering↗

Accurate and Efficient Parametric Model-Order Reduction for Turbulent Thermal Transport

This project produced new algorithms and software for low-cost exploration of turbulent thermal-fluids behavior under parametric variation by using reduced-order models (ROMs). The ROMs numerically solve the governing equations for fluid motion by using a small set of basis functions (typically, N=20-200 modes) to represent the solution. The base modes are computed as optimal combinations of solutions from expensive high-fidelity "anchor-point" solutions involving millions of unknowns, which are typically generated by solving the full Navier-Stokes equations on a supercomputer. The ROM solution is itself a combination of the base modes, where the basis coefficients are determined by evolving an NxN system of nonlinear equations. The overarching idea is to use the inexpensive ROM to predict solutions under conditions where the parameters differ from the anchor-point conditions. Several ingredients are required to make ROMs useful for thermal hydraulics analysis. These include: a stable and accurate ROM that is capable of reproducing the large-scale dynamics of turbulent flow, error indicators than can guide the choice of anchor points, and low-cost mechanisms for evaluating nonlinear terms in the reduced equations.

22 GENERAL STUDIES OF NUCLEAR REACTORS↗

Low-Flow Marine Hydrokinetic Turbine for Small Autonomous Unmanned Mobile Recharge Stations

A prototype low-flow marine current turbine for deployment from a small unmanned mobile floating platform has been developed for autonomously seeking and harnessing tidal/coastal currents. The support platform is an unmanned surface vehicle (USV), in the form of a catamaran with two electric outboard motors and with capabilities for autonomous navigation. The USV utilized is a WAM-V 16 vehicle that has been developed separately with support from the Office of Naval Research (ONR) [1]. The marine current turbine is based on a freestream waterwheel (FSWW), also known as an undershot waterwheel (FSWW), mounted on the stern of the USV. The concept of operation involves the USV autonomously navigating to a designated marine current resource. Upon arrival, the USV anchors itself, aligns with the current, and deploys the FSWW turbine using a custom cable-lift mechanism. The turbine harnesses the local current, and an onboard power-take-off (PTO) device converts the mechanical energy into electricity, which is stored in an onboard battery bank. When energy harvesting is completed, the turbine and the anchor are retrieved and the USV navigates to a selected location. These unmanned at-sea platforms can provide power to other unmanned maritime systems. Specifically, in this project, the power generated onboard can be used to charge aerial drones via a custom flight deck that has been developed for the USV. The recharging capabilities offered by a fleet of such strategically placed recharging stations can significantly benefit aerial drones operating in the maritime domain by eliminating the need to travel back and forth to land or ship based charging stations. The project has resulted in the development of subcomponents, including the FSWW turbine, a novel PTO, an automated anchoring system for the USV, an automated turbine deployment system, and a flight deck with capabilities onboard the USV for landing, direct-contact charging and takeoff of aerial drones. The design and development of these subsystems have culminated in the overall prototype marine hydrokinetic platform (MHK Platform, Fig. 1). Comprehensive lab and field testing have been conducted to validate the functionality and performance of the platform and its components. The project demonstrates the potential for autonomous, unmanned systems to harness renewable energy from marine currents, and provide sustainable power solutions for maritime applications such as coastal surveillance and environmental monitoring; shoreline mapping; search and rescue; oceanographic research; inspection and maintenance of offshore energy installations like wind turbines and oil rigs; oil spill response; maritime disaster response; and aerial surveys, as well as facilitation of data transfer drones and shore stations.

16 TIDAL AND WAVE POWER↗

An absolute recoil-carbon polarimeter for polarized light-ions at the BNL Booster

We describe a recoil-carbon polarimeter for the BNL Booster synchrotron capable of measuring the transverse polarization of both the polarized proton beam and the polarized 3 He ion beam throughout the acceleration cycle. The instrument addresses a critical gap in the BNL polarized beam program: no independent polarization measurement currently exists in the injector chain between the 200 MeV Linac polarimeter and the AGS. The proposed new polarimeter will provide continuous, absolute polarization measurements at the Booster stage, supplying additional anchor points for the polarization transmission through the accelerator chain. The polarimeter 9 is based on elastic pC and 3 He C scattering from a thin internal carbon target, with detection of the recoil 12 C nucleus in a fixed six-station silicon detector ring. A single detector geometry provides continuous kinematic coverage from injection to extraction for both beam species without mechanical adjustment. For pC scattering, the available data and the Bonin parametrization provide a well-established basis for estimating the analyzing power and figure of merit over the Booster energy range; for 3 He C the sole experimental anchor is a measurement at 443 MeV, and the Booster polarimeter itself is identified as the instrument to map the analyzing power across the remainder of the acceleration ramp by a ramp-and-return calibration that anchors the absolute 3 He polarization scale to a few percent. Provision for future deuteron polarimetry is incorporated in the chamber design without modification to the existing geometry. With appropriate detector upgrades, the same recoil-carbon technique can be extended to deuteron beams, for which analyzing power data are already available, and, with future analyzing-power measurements, also to 6 Li and 7 Li beams, making the polarimeter a versatile instrument for the full range of light polarized ion species anticipated at BNL.

43 PARTICLE ACCELERATORS↗

Tiedown Bracket

Tiedown bracket secured to concrete slab with lag anchor and lag bolt. A trailer or other heavy equipment can be anchored by tethering it to strapping bolt. When bracket is no longer needed, it can be removed, leaving behind only lag anchor. Bracket is easily installed and removed without damage to concrete slab.

Mashburn, D.↗

Test plan pressure fed thrust chamber technology

Aerojet is developing the technology for the design of a reliable, low cost, efficient, and lightweight LOX/RP-1 pressure fed engine. This technology program is a direct result of Aerojet's liquid rocket booster (LRB) study and previous NASA studies that identified liquid engines using high bulk density hydrocarbon fuels as very attractive for a space transportation system (STS). Previous large thrust LOX/RP-1 engine development programs were characterized by costly development problems due to combustion instability damage. The combustion stability solution was typically obtained through trial and error methods of minimizing instability damage by degrading engine performance. The approach to this program was to utilize existing and newly developed combustion analysis models and design methodology to create a thrust chamber design with features having the potential of producing reliable and efficient operation. This process resulted in an engine design with a unique high thrust-per-element OFO triplet injector utilizing a low cost modular approach. Cost efficient ablative materials are baselined for the injector face and chamber. Technology demonstration will be accomplished through a hot fire test program using appropriately sized subscale hardware. This subscale testing will provide a data base to supplement the current industry data bank and to anchor and validate the applied analysis models and design methodology. Once anchored and validated, these analysis models and design methodology can be applied with greatly increased confidence to design and characterize a large scale pressure fed LOX/RP-1 thrust chamber. The objective of this test program is to generate a data base that can be used to anchor and validate existing analysis models and design methodologies and to provide early concept demonstration of a low cost, efficient LOX/RP-1 thrust chamber. Test conditions and hardware instrumentation were defined to provide data sufficient to characterize combustion stability, performance, and thermal operation over a wide thrust chamber throttling range.

Dunn, Glenn↗

Human-Computer Interaction with Medical Decisions Support Systems

Decision Support Systems (DSSs) have been available to medical diagnosticians for some time, yet their acceptance and use have not increased with advances in technology and availability of DSS tools. Medical DSSs will be necessary on future long duration space missions, because access to medical resources and personnel will be limited. Human-Computer Interaction (HCI) experts at NASA's Human Factors and Ergonomics Laboratory (HFEL) have been working toward understanding how humans use DSSs, with the goal of being able to identify and solve the problems associated with these systems. Work to date consists of identification of HCI research areas, development of a decision making model, and completion of two experiments dealing with 'anchoring'. Anchoring is a phenomenon in which the decision maker latches on to a starting point and does not make sufficient adjustments when new data are presented. HFEL personnel have replicated a well-known anchoring experiment and have investigated the effects of user level of knowledge. Future work includes further experimentation on level of knowledge, confidence in the source of information and sequential decision making.

Adolf, Jurine A.↗

Modeling of SiC Lateral Resonant Devices Over a Broad Temperature Range

Finite-element analysis (FEA) modal results of 3C-SiC lateral resonant devices anchored to a Si substrate are presented as resonant frequency versus temperature. The suspended elements are etched from a 2 micron, 3C-SiC film grown at 1600 K on a 500 micron-thick, Si substrate. The analysis includes, temperature-dependent properties, shape change due to volume expansion with temperature, and thermal stress caused by differential thermal expansion of different materials. Two designs are considered: type I has anchor locations close to the geometric centroid and a small shuttle; type 11 has a large shuttle with anchors far from the centroid, The resonant frequency decreases approximately 3.5% over a 1000 K temperature increase for the type-I device, and behaves according to theory. The resonant frequency of the type-11 device decreases by 2% over the first 400 K, then rises slightly over the remaining 600 K. This device deviates from theory because of the high thermal stress induced in the beams. The thermal stress is caused by the differential thermal expansion of the suspended element relative to the substrate. The results show that the device geometry must be properly chosen if the resonant frequency of that device will be used to calculate the temperature coefficient of Young's modulus. These results apply only to resonators of one material on a substrate of a different material.

DeAnna, Russell G.↗

Numerical Simulation of an Enclosed Laminar Jet Diffusion Flame in Microgravity Environment: Comparison with ELF Data

Enclosed diffusion flames are commonly found in practical combustion systems, such as the power-plant combustor, gas turbine combustor, and jet engine after-burner. In these systems, fuel is injected into a duct with a co-flowing or cross-flowing air stream. The diffusion flame is found at the surface where the fuel jet and oxygen meet, react, and consume each other. In combustors, this flame is anchored at the burner (i.e., fuel jet inlet) unless adverse conditions cause the flame to lift off or blow out. Investigations of burner stability study the lift off, reattachment, and blow out of the flame. Flame stability is strongly dependent on the fuel jet velocity. When the fuel jet velocity is sufficiently low, the diffusion flame anchors at the burner rim. When the fuel jet velocity is increased, the flame base gradually moves downstream. However, when the fuel jet velocity increases beyond a critical value, the flame base abruptly jumps downstream. When this "jump" occurs, the flame is said to have reached its lift-off condition and the critical fuel jet velocity is called the lift-off velocity. While lifted, the flame is not attached to the burner and it appears to float in mid-air. Flow conditions are such that the flame cannot be maintained at the burner rim despite the presence of both fuel and oxygen. When the fuel jet velocity is further increased, the flame will eventually extinguish at its blowout condition. In contrast, if the fuel jet velocity of a lifted flame is reduced, the flame base moves upstream and abruptly returns to anchor at the burner rim. The fuel jet velocity at reattachment can be much lower than that at lift off, illustrating the hysteresis effect present in flame stability. Although there have been numerous studies of flame stability, the controlling mechanisms are not well understood. This uncertainty is described by Pitts in his review of various competing theories of lift off and blow out in turbulent jet diffusion flames. There has been some research on the stability of laminar flames, but most studies have focused on turbulent flames. It is also well known that the airflow around the fuel jet can significantly alter the lift off, reattachment and blow out of the jet diffusion flame. Buoyant convection is sufficiently strong in 1-g flames that it can dominate the flow-field, even at the burner rim. In normal-gravity testing, it is very difficult to delineate the effects of the forced airflow from those of the buoyancy-induced flow. Comparison of normal-gravity and microgravity flames provides clear indication of the influence of forced and buoyant flows on the flame stability. The overall goal of the Enclosed Laminar Flames (ELF) investigation (STS-87/USMP-4 Space Shuttle mission, November to December 1997) is to improve our understanding of the effects of buoyant convection on the structure and stability of co-flow diffusion flame, e.g., see http://zeta.lerc.nasa.gov/expr/elf.htm. The ELF hardware meets the experiment hardware limit of the 35-liter interior volume of the glovebox working area, and the 180x220-mm dimensions of the main door. The ELF experiment module is a miniature, fan-driven wind tunnel, equipped with a gas supply system. A 1.5-mm diameter nozzle is located on the duct's flow axis. The cross section of the duct is nominally a 76-mm square with rounded corners. The forced air velocity can be varied from about 0.2 to 0.9 m/s. The fuel flow can be set as high as 3 std. cubic centimeter (cc) per second, which corresponds to a nozzle exit velocity of up to 1.70 m/s. The ELF hardware and experimental procedure are discussed in detail in Brooker et al. The 1-g test results are repeated in several experiments following the STS-87 Mission. The ELF study is also relevant to practical systems because the momentum-dominated behavior of turbulent flames can be achieved in laminar flames in microgravity. The specific objectives of this paper are to evaluate the use reduced model for simulation of flame lift-off and blowout.

Jia, Kezhong↗

Haughton-Mars Project/NASA 2006 Lunar Medical Contingency Simulation: Equipment and Methods for Medical Evacuation of an Injured Crewmember

Introduction: Achieving NASA's Space Exploration Vision scientific objectives will require human access into cratered and uneven terrain for the purpose of sample acquisition to assess geological, and perhaps even biological features and experiments. Operational risk management is critical to safely conduct the anticipated tasks. This strategy, along with associated contingency plans, will be a driver of EVA system requirements. Therefore, a medical contingency EVA scenario was performed with the Haughton-Mars Project/NASA to develop belay and medical evacuation techniques for exploration and rescue respectively. Methods: A rescue system to allow two rescuer astronauts to evacuate one in incapacitated astronaut was evaluated. The systems main components were a hard-bottomed rescue litter, hand-operated winch, rope, ground picket anchors, and a rover-winch attachment adapter. Evaluation was performed on 15-25deg slopes of dirt with embedded rock. The winch was anchored either by adapter to the rover or by pickets hammered into the ground. The litter was pulled over the surface by rope attached to the winch. Results: The rescue system was utilized effectively to extract the injured astronaut up a slope and to a waiting rover for transport to a simulated habitat for advanced medical care, although several challenges to implementation were identified and overcome. Rotational stabilization of the winch was found to be important to get maximize mechanical advantage from the extraction system. Discussion: Further research and testing needs to be performed to be able to fully consider synergies with the other Exploration surface systems, in conducting contingency operations. Structural attachment points on the surface EVA suits may be critical to assist in incapacitated evacuation. Such attach points could be helpful in microgravity incapacitated crewmember transport as well. Wheeled utility carts or wheels that may be attachable to a litter may also aid in extraction and transport. Utilizing parts of the rover (e.g. seats) to deploy as a litter may be considered. Testing in simulated 1/6-g to determine feasibility of winch operation and anchor establishment will further reduce implementation uncertainties.

Chappell, S. P.↗

Experimental Evaluation of the Thermal Performance of a Water Shield for a Surface Power Reactor

A water based shielding system is being investigated for use on initial lunar surface power systems. The use of water may lower overall cost (as compared to development cost for other materials) and simplify operations in the setup and handling. The thermal hydraulic performance of the shield is of significant interest. The mechanism for transferring heat through the shield is natural convection. Natural convection in a representative lunar surface reactor shield design is evaluated at various power levels in the Water Shield Testbed (WST) at the NASA Marshall Space Flight Center. The experimental data from the WST is used to anchor a CFD model. Performance of a water shield on the lunar surface is then predicted by CFD models anchored to test data. The accompanying viewgraph presentation includes the following topics: 1) Testbed Configuration; 2) Core Heater Placement and Instrumentation; 3) Thermocouple Placement; 4) Core Thermocouple Placement; 5) Outer Tank Thermocouple Placement; 6) Integrated Testbed; 7) Methodology; 8) Experimental Results: Core Temperatures; 9) Experimental Results; Outer Tank Temperatures; 10) CFD Modeling; 11) CFD Model: Anchored to Experimental Results (1-g); 12) CFD MOdel: Prediction for 1/6-g; and 13) CFD Model: Comparison of 1-g to 1/6-g.

Pearson, J. Boise↗

Social-Cognitive Biases in Simulated Airline Luggage Screening

This study illustrated how social cognitive biases affect the decision making process of air1ine luggage screeners. Participants (n = 96) performed a computer simulated task to detect hidden weapons in 200 x-ray images of passenger luggage. Participants saw each image for two (high time pressure) or six seconds (low time pressure). Participants observed pictures of the "passenger" who owns the luggage . The "pre-anchor group" answered questions about the passenger before the luggage image appeared, the "post-snchor" group answered questions after the luggage appeared, and the "no-anchor group" answered no questions. Participants either stopped or did not stop the bag. and rated their confidence in their decision. Participants under high time pressure had lower hit rates and higher false alarms, Significant differences between the pre-, no-, and post-anchor groups were based on the gender and race of the passengers. Participants had higher false alarm rates in response to male than female passengers.

Brown, Jeremy R.↗

Pterodactyl: Thermal Protection System Design Methodology for a Flap Control System

As interest in non-traditional entry vehicles continues to grow, the need for a Thermal Protection System (TPS) analysis approach that can account for entry solutions with changing geometry and complicated flow dynamics becomes invaluable. The NASA Space Technology Mission Directorate (STMD) Pterodactyl project aims to accomplish this through examination of a flap controlled Adaptable, Deployable Entry Placement Technology (ADEPT)-style Deployable Entry Vehicle (DEV). This paper details an improved methodology for modeling the aerothermodynamic environment and initial TPS design for a flap control system integrated with a symmetric DEV. Improvements include i) the addition of an anchoring process that uses an increased fidelity aerodynamic solution to anchor the aerothermal environment predictions and ii) increased surface resolution, for the 1D heat transfer analysis, to isolate the hottest area on the flap that will require the thickest TPS. It was found that the anchoring process provided an improved aerothermal environment prediction. Additionally, this analysis demonstrated that there is a need for increased surface resolution in the 1D thermal analysis since the predicted location of the hottest point was significantly different than the location identified using very coarse surface resolution.

Zane B Hays↗

Trajectory Engineering with Modular Patched Conics for Entry Systems and TPS (TEMPEST)

Brief Presenter Biography (35 word limit): Bohdan Wesely is an Aerospace Engineer in the Entry Systems and Technology Division at Ames. He has worked on a variety of projects for NASA including integrated TPS (thermal protection system) flight hardware deliveries and testing services for commercial partners. Introduction: TEMPEST is a new trajectory analysis framework that is designed to fill the gap between dedicated flight mechanics tools and aerothermal and TPS sizing tools. The project started as an SJSU master’s thesis and has since evolved into a general conceptual design tool capable of studying a wide variety of entry problems. Development is ongoing in the Entry Systems and Technology Division at NASA ARC. Why TEMPEST: Space missions involving entry into a planetary atmosphere involve a series of unique requirements across multiple disciplines. Whether it is traditional entry descent and landing (EDL), or aerocapture, the vehicle must navigate to its target landing location or orbit state, and the TPS must protect the payload during entry. The design process typically involves iterative handoffs between various flight mechanics, flow solver, and material response level tools. During the early conceptual phase, a wide variety of feasible trajectories are simulated in a Monte Carlo scenario which broadly satisfy the mission or landing requirements. Next, computational fluid dynamics (CFD), direct simulation Monte Carlo (DSMC), and other flow solver analyses are performed at various key trajectory points to generate an aero-database, heating and TPS design requirements also emerge at this stage. At this point, with updated aerodynamics from the various flow solvers, trajectories can be re-run, this in turn can change the required freestream conditions for the CFD tools, and as a project progresses, these analyses converge, and uncertainty is reduced. However, there is always a “hand-off” occurring between two inherently coupled phenomena. Analysis Description: One of the goals with TEMPEST is to use a variety of first principles estimation methods coupled with an atmosphere model to predict vehicle aerothermodynamics across the entire flight regime while propagating a 3 or 6 degree of freedom (DoF) trajectory. Aerodynamics methods include modified Newtonian, Maxwell and Cercignani- Lampis-Lord (CLL) for continuum, transitional, and free molecular flow regimes. Aerothermodynamics include boundary layer and reference enthalpy methods, and Mutation++ for non-equilibrium chemistry modeling. TEMPEST is also capable of stitching multiple trajectory segments together to study mission scenarios like multi-pass aerocapture and aero-gravity assists. Most of the program is implemented in MATLAB using modern system objects, it relies on several C++ shared libraries for supporting tools like Gmsh, the Global Reference Atmospheric Model (GRAM), and Mutation++. The various first principles aerothermal estimation methods are discretized across either a structured axisymmetric panel mesh or an unstructured tri-mesh generated from an open-source tool such as Gmsh, this allows solutions on the same mesh to be compared across tools such as CB-Aero. CFD Coupling. A physics-aware, gaussian process CFD anchoring scheme is proposed to adjust the various first principles methods as a CFD database is populated. One goal for this anchoring module is to inform the project where CFD should be run. Full knowledge of the entire trajectory, atmosphere, and aerothermodynamics allows for easier identification of high sensitivity areas and uncertainty quantification. While the first principles effects are well known and proven accurate in existing tools such as CB- Aero and Cart3D, a physics aware CFD anchoring scheme increases tool credibility across a project lifecycle. Material Response Modeling. Correct TPS sizing is critical for optimizing mass for science payloads and ensuring mission success. The process typically involves a thermal analysis along the trajectory with surface heating environments as a boundary condition. Several design constraints are maximum bondline temperature and maximum recession with various margining techniques. The material response tool FIAT, developed out of NASA Ames, is currently being integrated into the TEMPEST environment. TPS recession, shape change, mass loss, and mass property alteration are all factors that can perturb an entry trajectory. For missions like Mars 2020, recession was minimal and was safely handled separately as a post process. For missions such as Jupiter Galileo with a high TPS mass fraction or asteroid entries, recession plays a major role. The proposed fully coupled scheme is to use an epoch-based approach where the trajectory integration is halted after a recession threshold, the energy balance and FIAT are solved at each panel, the mesh, aerodynamics, and mass properties are updated, and the trajectory continues. Several computational tradeoffs have been made during the development of TEMPEST to limit the cost of a single trajectory and preserve its utility as a conceptual, rapid iteration tool. Conclusion: Development of TEMPEST is ongoing and the project is still in its infancy. This talk aims to showcase its unique capabilities to support future NASA entry systems missions.

Bohdan O Wesely↗

Innovative Deep-Water Mooring Systems for Floating Wind Farms (DeepFarm): Cooperative Research and Development (Final Report)

The DeepFarm project, led by Principle Power Inc (PPI), focused on design, modeling and development of innovative mooring and anchoring systems for semi-submersible floating wind turbine platforms in a deep water, commercial-size wind farm. It explored the possibility of sharing anchors amongst the floating platforms within the farm, and considers site conditions representative of the West Coast and Hawaii, with a variety of water depths, as case studies to ensure that the sensitivity to water depth and park size are well understood. NREL and PPI worked together to develop and apply NREL's computational tools for modeling floating wind farm dynamics and costs.

17 WIND ENERGY↗

Convergent behavior of extended stalk regions from staphylococcal surface proteins with widely divergent sequence patterns

Staphylococcus epidermidis and Staphylococcus aureus are highly problematic bacteria in hospital settings. A major challenge is their ability to form biofilms on abiotic or biotic surfaces. Biofilms are well-organized, multicellular bacterial aggregates that resist antibiotic treatment and often lead to recurrent infections. Bacterial cell wall-anchored (CWA) proteins are important players in biofilm formation and infection. Many have putative stalk-like regions or regions of low complexity near the cell wall-anchoring motif. Recent work demonstrated the strong propensity of the stalk region of S. epidermidis accumulation-associated protein (Aap) to remain highly extended under solution conditions that typically induce compaction. This behavior is consistent with the expected function of a stalk-like region that is covalently attached to the cell wall peptidoglycan and projects the adhesive domains of Aap away from the cell surface. In this study, we evaluate whether the ability to resist compaction is a common theme among stalk regions from various staphylococcal CWA proteins. Circular dichroism spectroscopy was used to examine secondary structure changes as a function of temperature and cosolvents along with sedimentation velocity analytical ultracentrifugation, size-exclusion chromatography, and SAXS to characterize structural characteristics in solution. All stalk regions tested are intrinsically disordered, lacking secondary structure beyond random coil and polyproline type II helix, and they all sample highly extended conformations. Remarkably, the Ser-Asp dipeptide repeat region of SdrC exhibited nearly identical behavior in solution when compared to the Aap Pro/Gly-rich region, despite highly divergent sequence patterns, indicating conservation of function by various distinct staphylococcal CWA protein stalk regions.

59 BASIC BIOLOGICAL SCIENCES↗

Mechanistic Insights into Adsorptive and Catalytic Reactions from Controllable Distributions of Metal Cations (Pd, Pt, Ni, Cr, Cu) as [M‐OH] +1 /1Al or M +2 /2Al in Zeolites

Anchoring divalent metal ions in the same zeolite framework with similar Si/Al ratio selectively as zeolite-bound M +2 or [M +2 -OH] +1 cationic species enables critical comparison of the species’ intrinsic reactivity for industrially and fundamentally relevant reactions. H-BEA zeolites with similar Si/Al ratios but differing framework Al siting were used to anchored multiple divalent metal cations (Ni, Pd, Pt, Cr, Cu) in the zeolite micropores. State-of-the-art infrared (IR) spectroscopy, electron paramagnetic resonance (EPR) measurements, including two-dimensional pulsed HYSCORE EPR, extended X-ray absorption fine structure (EXAFS), and density functional theory (DFT) calculations together provide unambiguous evidence for the selective formation of divalent metal cations as M +2 /2Al species (for H-BEA prepared in the conventional hydroxide media), and [M +2 OH] +1 /1Al species for H-BEA prepared in HF. Solid-state proton-decoupled triple-quantum magic-angle spinning (3Q MAS) NMR measurements confirmed contrasting Al distributions in the two H-BEA zeolites, which led to a contrasting divalent cation speciation. The reactivities of the two cationic species were explored for catalytic and adsorptive applications in both organometallic homogeneous and heterogeneous catalysis. This work demonstrates their divergent reactivity in ethylene dimerization, ethylene oxidation (Wacker process), selective catalytic reduction (SCR) of NO, NO adsorption, and methane oxidation. Both M +2 /2Al and [M +2 OH] +1 /1Al cations are both active for ethylene dimerization, but [M +2 OH] +1 /1Al species show higher reaction rates for each Pd, Ni, Pt. [M +2 OH] +1 /1Al is active for acetaldehyde formation in Wacker ethylene oxidation. A new active site for ethylene oligomerization is proposed that possesses a terminal OH group (Cr-OH) in Phillips catalysts evident by a nearly inactive isolated Cr +2 /2Al species that contrast an active Cr─OH motif.

Divalent metal cations in a zeolite↗

Formative vs. Summative Dependence in Human Reliability Analysis

Dependence in human reliability analysis (HRA) is the concept that once an initial human error has occurred, subsequent human errors are more likely. The approach almost universally adopted in HRA was first introduced in the Technique for Human Error Rate Prediction (THERP). In THERP, calculated human error probabilities (HEPs) are adjusted for dependence in the final step of quantification. This final adjustment for dependence involves anchoring the HEP to a set of values corresponding to low through complete dependence. The effect is to increase the HEP. In this paper, we propose formative dependence, which occurs at the onset of quantification. We demonstrate that dependence should be considered earlier in the calculation process, because it is not actually necessary to calculate the HEP when it is subsequently overridden by the dependence anchors. By applying dependence first in the calculation, processing steps can be eliminated, making for more efficient analysis.

99 GENERAL AND MISCELLANEOUS↗