Multifunctional perennial production systems for bioenergy: performance and progress
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The emergence of the cellulosic bioeconomy requires not only adequate technological, economic, and policy advances, but also effective communication and coordination among the multiple stakeholders in the bioeconomy community. Aiming to facilitate stakeholder communication and collaboration for cellulosic bioeconomy growth, an agent-based model is developed as a community communication tool in this study. The tool simulates the behaviors and interactions of key stakeholders, as well as the responses and interactions between the stakeholders and biophysical, socioeconomic, and environmental conditions. The agent behavior rules are developed and validated based on behavior and economic theories and information attained from a stakeholder focus group study and a land use survey. A pilot study in a Central Illinois watershed shows stakeholder synergies, i.e., positive feedback loops across multiple stakeholders. The modeling tool's role in facilitating community communication is demonstrated via the focus group study. A comparison of policy scenarios suggests that the cellulosic bioeconomy development is fastest under a portfolio of policies combining the views of the multiple stakeholders. Critically, subsidy for small-scale bio-facilities is essential as it rapidly initializes an early market for cellulosic feedstocks and the adoption of Miscanthus. As a result, the communication tool is applied to bringing stakeholders and researchers together to develop shared visions, assess barriers, and explore solution portfolios to initialize and speed up the regional cellulosic bioeconomy.
A round robin study evaluating the analysis of biomass liquefaction oils (BLOs) from fast pyrolysis and hydrothermal liquefaction (HTL) was performed, involving fifteen laboratories in seven countries in order to assess the current status of analytical techniques for the determination of nitrogen, sulfur, and chlorine content in BLOs and evaluate potential differences in origin (i.e. fast pyrolysis vs HTL). The BLOs were produced from a range of feedstocks including pine, mixed softwoods, forest residues, micro-algae, miscanthus, and wheat straw to cover a variety in nitrogen, sulfur, and chlorine content and speciation. Nine samples were distributed, comprised of eight separate BLOs and one blind duplicate produced by five producers. The samples were analyzed for water, carbon, hydrogen, nitrogen, sulfur, and chlorine content. No analytical test method was mandated; laboratories were encouraged to utilize whichever method they determined would be most applicable, relying on the existing body of BLO literature as a guide. The results of this round robin study are presented in this paper. The results of the carbon, hydrogen, and water measurements as reference analyses had relative standard deviations (2.9%, 3.5, and 5.6%, respectively) that were comparable to those found in past round robin studies on fast pyrolysis bio-oil. The analysis of nitrogen, sulfur, and chlorine showed higher levels of variability. Laboratories mostly chose the same method for water, carbon, hydrogen and nitrogen determination whereas there were a variety of methods chosen for sulfur and chlorine determination. The results suggest that specific analytical methods for the determination of nitrogen, sulfur, and chlorine should be further refined to ensure reproducible and accurate results for BLO analysis due to their importance on emissions, material selection, and catalyst activity.
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Six years after replacing a maize/soybean cropping system, perennial grasses miscanthus (Miscanthus × giganteus) and switchgrass (Panicum virgatum), and a 28-species restored prairie increased particulate organic carbon in surface soils without increasing soil organic carbon (SOC). To resolve potential changes in the quantity and distribution of SOC, soils were resampled after seven to thirteen years to measure bulk density, carbon (C) content, and stable C isotopes to a depth of 1 m. SOC stocks increased between 1.75 and 2.5 Mg ha −1 year −1 in all perennial crops between 2008 and 2016 (nine growing seasons). Despite relatively low litter inputs and belowground biomass, the highest rate of SOC accrual was in restored prairie (2.5 Mg ha −1 year −1 ), followed by miscanthus (2.0 Mg ha −1 year −1 ) and switchgrass (1.75 Mg ha −1 year −1 ). The change in SOC in maize/soybean was not significant. After 2016, total SOC decreased in maize/soybean and miscanthus, resulting in slower overall rates of SOC accumulation over the full sampling period for miscanthus (0.8 Mg ha −1 year −1 ). The rate of SOC accumulation was greatest below 50 cm depth for restored prairie and switchgrass but in the top 10 cm for miscanthus. Stable isotope analysis showed 13 C enrichment in all depths of switchgrass soils, an indication of new organic C accumulation, but mixed results in all other crops. Planting perennial crops on land formerly in an annual maize/soybean cropping system can slow or reverse soil carbon losses, with the greatest increases in SOC from species-rich prairie.
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These data were collected in 2018 and 2019 at the University of Illinois Energy Farm (N 40.063607, W 88.206926). During each growing season, bulk and rhizosphere soil were collected from replicate Sorghum bicolor nitrogen use efficiency trial plots at three separate time points (approximately July 1, August 1, and September 1). We measured soil moisture, pH, soil nitrate and ammonium, potential nitrification, potential denitrification, and extracted and sequenced the V4 region of the 16S rRNA gene for microbial community analysis. All microbial sequence data is archived in the National Center for Biotechnology Information’s (NCBI) Sequence Read Archive (accession number SRP326979, project number PRJNA741261).
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Anaerobic membrane bioreactors (AnMBR) have been used for treating high-strength industrial wastewater at full-scale and the potential to use them for mainstream municipal wastewater treatment presents an important opportunity to turn energy-intensive plants into net-energy producers. However, several limitations of the AnMBR technology have prevented their adoption in the municipal wastewater industry, namely, high membrane cleaning energy demand and low membrane flux. This study demonstrated a novel AnMBR configuration that uses a commercially available cloth filter technology to address the key limitations of cleaning energy and membrane flux. The cloth filter anaerobic membrane bioreactor (CFAnMBR) is comprised of an anaerobic fixed-film bioreactor coupled with a cloth filter membrane with nominal pore size of 5 µm. The pilot CFAnMBR was operated for 150 days through the winter at a municipal wastewater plant in central Illinois (minimum/average influent temperature 5/13°C). The CFAnMBR increased membrane flux by more than 2 orders of magnitude (3,649 ± 1,246 L per meter squared per hour) and reduced cleaning energy demand by 78%—92% (0.0085 kWh/m 3 ) relative to previously reported AnMBR configurations. With the CFAnMBR, average chemical oxygen demand and total suspended solids removal were 66% and 91%, respectively, and were shown to be increased up to 88% and 96% by in-line coagulant dosing with ferric chloride. Average headspace methane yield was 154 mL CH 4 /g COD removed by the end of the study period with influent temperatures of 11°C± 4°C. The CFAnMBR resolves major limitations of AnMBR technology by employing a commercially-available technology already used for other municipal wastewater treatment applications.
These files contain data from soil and root samples use in this publication. The R script uses this data to perform the statistical analysis used in the publication.
Data from: Sorghum bicolor TX08001 nodal root tissue development gene expression profiling
Sorghum bicolor TX08001 Nodal Root Tissue Development Gene Expression Profiling
This study quantified root biomass distribution; root accrual of C, N, P, and K; and changes in soil organic carbon (SOC) associated with alley-cropped switchgrass (Panicum virgatum L.), prairie cordgrass (Spartina pectinata Bosc ex Link), intermediate wheatgrass [Thinopyrum intermedium (Host) Barkworth and Dewey ‘Rush’], and a native polyculture planted between rows of ‘NM6’ poplar (Populus maximowiczii × P. nigra) and ‘Fish Creek’ willow (Salix purpurea) at two Minnesota sites (Empire and Granada). After 4 yr since establishment, SOC declined at each site but was not influenced by species selection. NM6 poplar–prairie cordgrass systems had among the highest root biomass, C, and nutrient accrual, with up to 16.3 Mg root biomass, 7.0 Mg C, 175 kg N, 31 kg P, and 97 kg K ha -1 . Fine roots were the largest fraction of belowground biomass, although course roots were also a large fraction for poplar and prairie cordgrass. Tree roots extended to 6 m into the crop alley, although 85–89% were within 1 m of tree rows, depending on tree species. Crop fine root biomass was reduced up to 67% at 1 m from tree rows and 20% at 3.5 m and was up to 142% greater in willow than in poplar alleys. Total root C was predominated by poplars at Empire regardless of herbaceous crop type, whereas the proportions of tree and crop root C varied by crop at Granada. These results suggest that prairie cordgrass is well suited to alley cropping and that, due to the competitive ability of poplars, productivity, C sequestration, and nutrient accrual may be greater in willow systems in the long term.
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