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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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At least 217 records · Page 12

Low-Energy Microfocus X-Ray Source for Enhanced Testing Capability in the Stray Light Facility

Research toward high-resolution, soft x-ray optics (mirrors and gratings) necessary for the next generation large x-ray observatories requires x-ray testing using a low-energy x-ray source with fine angular size (<1 arcsecond). To accommodate this somewhat demanding requirement, NASA Marshall Space Flight Center (MSFC) has procured a custom, windowless low-energy microfocus (approximately 0.1 mm spot) x-ray source from TruFocus Corporation that mates directly to the Stray Light Facility (SLF). MSFC X-ray Astronomy team members are internationally recognized for their expertise in the development, fabrication, and testing of grazing-incidence optics for x-ray telescopes. One of the key MSFC facilities for testing novel x-ray instrumentation is the SLF. This facility is an approximately 100-m-long beam line equipped with multiple x-ray sources and detectors. This new source adds to the already robust compliment of instrumentation, allowing MSFC to support additional internal and community x-ray testing needs.

Gaskin, Jessica↗

A unique high heat flux facility for testing hypersonic engine components

This paper describes the Hot Gas Facility, a unique, reliable, and cost-effective high-heat-flux facility for testing hypersonic engine components developed at the NASA Lewis Research Center. The Hot Gas Facility is capable of providing heat fluxes ranging from 200 Btu/sq ft per sec on flat surfaces up to 8000 Btu/sq ft per sec at a leading edge stagnation point. The usefulness of the Hot Gas Facility for the NASP community was demonstrated by testing hydrogen-cooled structures over a range of temperatures and pressures. Ranges of the Reynolds numbers, Prandtl numbers, enthalpy, and heat fluxes similar to those expected during hypersonic flights were achieved.

Melis, Matthew E.↗

Plant root exudates and rhizosphere bacterial communities shift with neighbor context

A plant's neighborhood context can alter its interactions with other organisms, but little is known about how these dynamics occur belowground, especially with soil microbes. Microbial communities in rhizosphere soil are influenced by many factors, including abiotic conditions and root-derived signals. In particular, root exudates have strong effects on rhizosphere assembly, respond to changes in abiotic conditions, and help plants interact with neighbors. Therefore, we predicted that root exudates likely play a central role in neighbor-induced shifts in rhizosphere communities. We conducted a greenhouse experiment to test this and determine how the rhizosphere bacterial community of a focal plant, Panicum virgatum, changed when beside different neighbors, and whether these shifts were mediated by neighbor-induced changes in root exudation. We found that neighbor altered both focal plant exudates and rhizosphere community, and that changes were largest when the focal plant was beside the most competitive neighbor, Rudbeckia hirta, which reduced both focal plant growth and nitrogen uptake. Several factors contributed to neighbor impacts on rhizosphere assembly, including neighbor-induced changes in root exudates during nitrogen-limitation and microbial spillover from roots of larger neighbors. Using an additional soil incubation, we also found that these changes in exudates can have even greater effects on soil nutrients than on microbial assembly. Overall, we show that neighbors influence one another's microbiomes, and highlight neighbor-induced changes in root exudates as one mechanism through which this may occur. Furthermore, this work suggests that rhizosphere assembly may differ in mixed-species communities and thus emphasizes a need for microbiome studies that consider neighborhood context.

59 BASIC BIOLOGICAL SCIENCES↗

Facility Location Optimization Model for COVID-19 Resources

In response to anticipated resource shortfalls related to the treatment and testing of COVID-19, many communities are planning to build additional facilities to increase capacity. These facilities include field hospitals, testing centers, mobile manufacturing units, and distribution centers. In many cases, these facilities are intended to be temporary and are designed to meet an immediate need. When deciding where to place new facilities many factors need to be considered, including the feasibility of potential locations, existing resource availability, anticipated demand, and accessibility between patients and the new facility. In this project, a facility location optimization model was developed to integrate these key pieces of information to help decision makers identify the best place, or places, to build a facility to meet anticipated resource demands. The facility location optimization model uses the location of existing resources and the anticipated resource demand at each location to minimize the distance a patient must travel to get to the resource they need. The optimization formulation is presented below. The model was designed to operate at the county scale, where patients are grouped per county. This assumption can be modified to integrate other scales or include individual patients.

60 APPLIED LIFE SCIENCES↗

Acoustic Research under the Source Physics Experiment

The Source Physics Experiment series is a long-term research and development (R&D) effort under the U.S. Department of Energy’s National Nuclear Security Administration focused on improving the physical understanding of how chemical explosions generate seismoacoustic signals. Beginning in 2011, a series of subsurface chemical explosions in two different and highly contrasting geologies were conducted at the Nevada National Security Site in Nevada, USA with the objective of improving simulation and modeling approaches to explosion identification, yield estimation and other monitoring applications. The two executed phases of the series provide new explosion signature source data from a wide range of geophysical diagnostic equipment; recorded data from the test series is now openly available to the broader seismoacoustic community. This manuscript details the executed test series, deployed seismoacoustic networks, and summarizes major scientific achievements utilizing recorded signatures from the explosive tests.

45 MILITARY TECHNOLOGY, WEAPONRY, AND NATIONAL DEF↗

Universal Signal Conditioning Amplifier

The Technological Research and Development Authority (TRDA) and NASA-KSC entered into a cooperative agreement in March of 1994 to achieve the utilization and commercialization of a technology development for benefiting both the Space Program and U.S. industry on a "dual-use basis". The technology involved in this transfer is a new, unique Universal Conditioning Amplifier (USCA) used in connection with various types of transducers. The project was initiated in partnership with I-Net Corporation, Lockheed Martin Telemetry & Instrumentation (formerly Loral Test and Information Systems) and Brevard Community College. The project consists of designing, miniaturizing, manufacturing, and testing an existing prototype of USCA that was developed for NASA-KSC by the I-Net Corporation. The USCA is a rugged and field-installable self (or remotely)- programmable amplifier that works in combination with a tag random access memory (RAM) attached to various types of transducers. This summary report comprises performance evaluations, TRDA partnership tasks, a project summary, project milestones and results.

Kinney, Frank↗

PACE OCI Polarization Sensitivity Based on Pre-launch Testing

The Ocean Color Instrument (OCI) is a sensor on the upcoming Plankton, Aerosol, Cloud, ocean Ecosystem (PACE) mission, scheduled for launch in early 2024. OCI is a grating spectrometer with hyperspectral coverage from the ultraviolet (about 310 nm) to near-infrared (about 900 nm), with additional filtered channels in the short-wave infrared (940 nm – 2260 nm). This instrument will provide ocean color science data to continue the data sets collected by heritage sensors MODIS, SeaWiFs, and VIIRS, but with increased spectral coverage and improved accuracy. In order to achieve the high levels of accuracy demanded by the science community, a rigorous ground test program was conducted to calibrate the instrument and ensure that the calibration can be transferred to on-orbit operations. Some calibration parameters can only be measured during pre-launch testing; one such parameter is the polarization sensitivity. Polarization testing measured the Mueller matrix components needed to determine the polarization sensitivity for all spectral bands for a series of telescope scan angles covering the expected on-orbit scan range. Results indicate that the sensitivity is below 0.6 % except at the shortest wavelengths (less than 340 nm) and was characterized to better than 0.1 % above 340 nm. This indicates that any polarized scenes measured on orbit can be corrected for with a high degree of confidence.

PACE↗

PACE OCI Polarization Sensitivity Based on Pre-launch Testing

The Ocean Color Instrument (OCI) is a sensor on the upcoming Plankton, Aerosol, Cloud, ocean Ecosystem (PACE) mission, scheduled for launch in early 2024. OCI is a grating spectrometer with hyperspectral coverage from the ultraviolet (about 310 nm) to near-infrared (about 900 nm), with additional filtered channels in the short-wave infrared (940 nm – 2260 nm). This instrument will provide ocean color science data to continue the data sets collected by heritage sensors MODIS, SeaWiFs, and VIIRS, but with increased spectral coverage and improved accuracy. In order to achieve the high levels of accuracy demanded by the science community, a rigorous ground test program was conducted to calibrate the instrument and ensure that the calibration can be transferred to on-orbit operations. Some calibration parameters can only be measured during pre-launch testing; one such parameter is the polarization sensitivity. Polarization testing measured the Mueller matrix components needed to determine the polarization sensitivity for all spectral bands for a series of telescope scan angles covering the expected on-orbit scan range. Results indicate that the sensitivity is below 0.6 % except at the shortest wavelengths (less than 340 nm) and was characterized to better than 0.1 % above 340 nm. This indicates that any polarized scenes measured on orbit can be corrected for with a high degree of confidence.

PACE↗

Standard test case for a low-speed, turbulent junction vortex flow

The mean flow structure upstream, around within, and in the near wake of a turbulent junction or horseshoe vortex is reported for an incompressible subsonic flow. Measurements of the primitive variables of velocity and pressure are reported on all surfaces bounding the region of the vortex flow and on three transverse and one streamwise plane within the flowfield itself for comparisons between the measured and any calculated flow variables. Detailed surface flow visualizations and some direct force measurements of surface shear stress are also available. The data is a highly detailed, coherent, self-consistent set offered to the computational fluid mechanics community as a standard test case for the evaluation of the capability of numerical solvers intended for predicting the flowfield in such a complex, separated, three-dimensional turbulent flow. This data base is available for copying to user supplied tapes or for transmission via BITNET, as well as in two National Technical Information Service (NTIS) reports.

Pierce, F. J.↗

Integrated Renewable Energy Systems

Pacific Northwest National Laboratory (PNNL) operates the Department of Energy’s (DOE) only dedicated marine laboratory at the PNNL-Sequim campus. PNNL is leading research in the blue economy and marine energy applications and building collaboration between DOE and multiple partners in the state of Washington and beyond. With Washington State support, the Integrated Renewable Energy System (IRES) demonstration testbed proposed here will advance research by developing and testing renewable energy production, management, and use for multiple marine applications (e.g., ocean observations, underwater vehicles, aquaculture). It will also advance energy resiliency for coastal communities by developing an integrated renewable energy test platform that will model how multiple renewable energy resources could power shoreline businesses or communities (Figure 1). The test bed will demonstrate how different renewable systems can be integrated to reduce carbon emissions and contribute to a net zero emissions site and provide lessons, controls, and protocols that will help to expand energy options for shoreline and maritime businesses. The integrated system is expected to serve as a test bed for shoreline power and ocean energy technologies for years to come.

32 ENERGY CONSERVATION, CONSUMPTION, AND UTILIZATI↗

Microbial community response to a decade of simulated global changes depends on the plant community

Global changes such as increased drought and atmospheric nitrogen deposition perturb both the microbial and plant communities that mediate terrestrial ecosystem functioning. However, few studies consider how microbial responses to global changes may be influenced by interactions with plant communities. To begin to address the role of microbial–plant interactions, we tested the hypothesis that the response of microbial communities to global change depends on the plant community. We characterized bacterial and fungal communities from 395 plant litter samples taken from the Loma Ridge Global Change Experiment, a decade-long global change experiment in Southern California that manipulates rainfall and nitrogen levels across two adjacent ecosystems, a grassland and a coastal sage scrubland. The differences in bacterial and fungal composition between ecosystems paralleled distinctions in plant community composition. In addition to the direct main effects, the global change treatments altered microbial composition in an ecosystem-dependent manner, in support of our hypothesis. The interaction between the drought treatment and ecosystem explained nearly 5% of the variation in bacterial community composition, similar to the variation explained by the ecosystem-independent effects of drought. Unexpectedly, we found that the main effect of drought was approximately four times as strong on bacterial composition as that of nitrogen addition, which did not alter fungal or plant composition. Overall, the findings underscore the importance of considering plant–microbe interactions when considering the transferability of the results of global change experiments across ecosystems.

54 ENVIRONMENTAL SCIENCES↗

Measurements of soil protist richness and community composition are influenced by primer pair, annealing temperature, and bioinformatics choices

ABSTRACT Protists are a diverse and understudied group of microbial eukaryotic organisms especially in terrestrial environments. Advances in molecular methods are increasing our understanding of the distribution and functions of these creatures; however, there is a vast array of choices researchers make including barcoding genes, primer pairs, PCR settings, and bioinformatic options that can impact the outcome of protist community surveys. Here, we tested four commonly used primer pairs targeting the V4 and V9 regions of the 18S rRNA gene using different PCR annealing temperatures and processed the sequences with different bioinformatic parameters in 10 diverse soils to evaluate how primer pair, amplification parameters, and bioinformatic choices influence the composition and richness of protist and non-protist taxa using Illumina sequencing. Our results showed that annealing temperature influenced sequencing depth and protist taxon richness for most primer pairs, and that merging forward and reverse sequencing reads for the V4 primer pairs dramatically reduced the number of sequences and taxon richness of protists. The data sets of primers that targeted the same 18S rRNA gene region (e.g., V4 or V9) had similar protist community compositions; however, data sets from primers targeting the V4 18S rRNA gene region detected a greater number of protist taxa compared to those prepared with primers targeting the V9 18S rRNA region. There was limited overlap of protist taxa between data sets targeting the two different gene regions (80/549 taxa). Together, we show that laboratory and bioinformatic choices can substantially affect the results and conclusions about protist diversity and community composition using metabarcoding. IMPORTANCE Ecosystem functioning is driven by the activity and interactions of the microbial community, in both aquatic and terrestrial environments. Protists are a group of highly diverse, mostly unicellular microbes whose identity and roles in terrestrial ecosystem ecology have been largely ignored until recently. This study highlights the importance of choices researchers make, such as primer pair, on the results and conclusions about protist diversity and community composition in soils. In order to better understand the roles protist taxa play in terrestrial ecosystems, biases in methodological and analytical choices should be understood and acknowledged.

Biotechnology & Applied Microbiology↗

Discovery and Vetting of Exoplanets. I. Benchmarking K2 Vetting Tools

We have adapted the algorithmic tools developed during the Kepler mission to vet the quality of transit-like signals for use on the K2 mission data. Using the four sets of publicly available light curves at MAST, we produced a uniformly vetted catalog of 772 transiting planet candidates from K2 as listed at the NASA Exoplanet Archive in the K2 Table of Candidates. Our analysis marks 676 of these as planet candidates and 96 as false positives. All confirmed planets pass our vetting tests. Sixty of our false positives are new identifications, effectively doubling the overall number of astrophysical signals mimicking planetary transits in K2 data. Most of the targets listed as false positives in our catalog show either prominent secondary eclipses, transit depths suggesting a stellar companion instead of a planet, or significant photocenter shifts during transit. We packaged our tools into the open-source, automated vetting pipeline Discovery and Vetting of Exoplanets (DAVE), designed to streamline follow-up efforts by reducing the time and resources wasted observing targets that are likely false positives. DAVE will also be a valuable tool for analyzing planet candidates from NASA's TESS mission, where several guest-investigator programs will provide independent light-curve sets—and likely many more from the community. We are currently testing DAVE on recently released TESS planet candidates and will present our results in a follow-up paper.

Kostov, Veselin B.↗

Environmental harshness mediates the relationship between aboveground and belowground communities in Antarctica

Linkages between aboveground and belowground communities are a key but globally under-researched component of responses to environmental change. Given the logistical complications to studying these relationships, much of our knowledge derives from laboratory experiments and localized field studies which have so far yielded inconsistent results. Because environmental factors may alter relationships between above- and belowground communities, there is a need for broad-scale field studies testing these interactions. The Antarctic Peninsula provides an ideal test setting, given the relatively simple communities both above- and belowground. The Peninsula is also experiencing rapid environmental changes, including alterations in species diversity and distribution both above- and belowground. Thus, an improved understanding of the broad-scale consequences of altered environments and vegetation communities for the soil microbiome is of high priority. To determine the nature and strength of the relationship between in situ plant and soil communities across a broad spatial scale and range of environmental conditions, we sampled soil communities at 9 locations (spanning 60–72°S along the Scotia Arc and Antarctic Peninsula) beneath the major aboveground habitats (moss, grass, lichen, algae and bare soil). We measured a comprehensive suite of soil physicochemical properties, microbial (bacterial and fungal) diversity and composition, and invertebrate abundance and community composition to determine the relationships between plant and soil communities. Furthermore, our results suggest that, with increased environmental severity, plant cover types become more important for influencing the physicochemical soil environment, and therefore the soil microbial communities. Although we found site-specific relationships, broad-scale patterns reveal significant differences among bare soils and vegetated soils, particularly soils beneath grass and moss. This suggests that expansion of vegetation communities under current climate warming projections will be accompanied by shifts in the soil microbiome, with important implications for the ecosystem functioning with which they are associated.

54 ENVIRONMENTAL SCIENCES↗

Siting solar thermal power experiments

The Small Community Solar Thermal Power Experiment will test a point-focusing, distributed receiver system in a small community, electric utility application. Site planning is becoming increasingly important for solar experiments as well as all kinds of development due to increased competition for desirable sites and increased complexity of regulatory requirements. Siting issues can be categorized as: (1) resources and physical environmental at this site; (2) acquisition, permits and regulations; and (3) development requirements and costs. These issues are addressed with respect to the unique requirements of solar thermal power experiments.

Holbeck, H. J.↗

Site participation in the small community experiment

The Small Community Solar Thermal Experiment, planned to test a small, developmental solar thermal power plant in a small community application, is assessed. The baseline plan is to install a field of parabolic dishes with distributed generation to provide 1 MWe of experimental power. Participation by the site proposer is an integral element of the experiment; the proposer will provide a ten-acre site, a connection to the electrical distributional system serving the small community, and various services. In addition to the primary participant, site study efforts may be pursued at as many as five alternative sites.

Holbeck, H. J.↗

Data for Immediate Impacts of Soybean Cover Crop on Bacterial Community Composition and Diversity in Soil Under Long-Term Saccharum Monoculture

Saccharum yield decline results from long-term monoculture practices. Changes in cropping management can improve soil health and productivity. Below-ground bacterial community diversity and composition across soybean (Glycine max (L.) Merr) cover crop, Saccharum monoculture (30+ year) and fallowed soil were determined. Near full length (~1,400 base pairs) of 16S rRNA gene sequences were extracted from the rhizospheres of sugarcane and soybean and fallowed soil were compared. Higher soil bacterial diversity was observed in the soybean cover crop than sugarcane monoculture across all measured indices (observed operationational taxonomic units, Chao1, Shannon, reciprocal Simpson and Jackknife). Acidocateria, Proteobacteria, Bacteroidetes and Planctomycetes were the most abundant bacterial phyla across the treatments. Indicator species analysis identified nine indicator phyla. Planctomycetes, Armatimonadetes and candidate phylum FBP were associated with soybean; Proteobacteria and Firmicutes were linked with sugarcane and Gemmatimonadetes, Nitrospirae, Rokubacteria and unclassified bacteria were associated with fallowed soil. Non-metric multidimensional scaling analysis showed distinct groupings of bacterial operational taxonomic units (97% identity) according to management system (soybean, sugarcane or fallow) indicating compositional differences among treatments. This is confirmed by the results of the multi-response permutation procedures (A = 0.541, p = 0.00045716). No correlation between soil parameters and bacterial community structure was observed according to Mantel test (r = 211865, p = 0.14). Use of soybean cover-crop fostered bacterial diversity and altered community structure. This indicates cover crops could have a restorative effect and potentially promote sustainability in long-term Saccharum production systems.

Field Data↗

Needle bacterial community structure across the species range of limber pine

Abstract Bacteria on and inside leaves can influence forest tree health and resilience. The distribution and limits of a tree species’ range can be influenced by various factors, with biological interactions among the most significant. We investigated the processes shaping the bacterial needle community across the species distribution of limber pine, a widespread Western conifer inhabiting a range of extreme habitats. We tested four hypotheses: (i) Needle community structure varies across sites, with site-specific factors more important to microbial assembly than host species selection; (ii) dispersal limitation structures foliar communities across the range of limber pine; (iii) the relative significance of dispersal and selection differs across sites in the tree species range; and (iv) needle age structures bacterial communities. We characterized needle communities from the needle surface and tissue of limber pine and co-occurring conifers across 16 sites in the limber pine distribution. Our findings confirmed that site characteristics shape the assembly of bacterial communities across the host species range and showed that these patterns are not driven by dispersal limitation. Furthermore, the strength of selection by the host varied by site, possibly due to differences in available microbes. Our study, by focusing on trees in their natural setting, reveals real needle bacterial dynamics in forests, which is key to understanding the balance between stochastic and deterministic processes in shaping forest tree-microbe interactions. Such understanding will be necessary to predict or manipulate these interactions to support forest ecosystem productivity or assist plant migration and adaptation in the face of global change.

59 BASIC BIOLOGICAL SCIENCES↗