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At least 217 records · Page 12

Single cell RNA sequencing reveals shifts in cell maturity and function of endogenous and infiltrating cell types in response to acute intervertebral disc injury

Intervertebral disc (IVD) degeneration contributes to disabling back pain. Degeneration can be initiated by injury and progressively leads to an irreversible loss of cells and function. IVD function restoration through cell replacement therapies have had limited success due to knowledge gaps in the critical cell populations important for repair. Here, in this study, we used single cell RNA sequencing to identify the transcriptional changes of IVD resident and infiltrating cell populations from Control and Injured coccygeal IVDs extracted from 12-week-old female C57BL/6J mice 7 days post injury. Clustering, gene ontology, and pseudotime trajectory analyses determined transcriptomic divergences with injury, flow cytometry identified they types of infiltrating immune cells, and immunofluorescence was utilized to define mesenchymal stem cell (MSC) localization. We identified 11 distinct clusters that included IVD, immune, vascular cells, and MSCs. Differential gene expression analysis determined that Outer Annulus Fibrosus, Neutrophils, Saa2-High MSCs, Macrophages, and Krt18 + Nucleus Pulposus (NP) cells were the major drivers of transcriptomic differences between Control and Injured cells. Gene ontology revealed that the most upregulated biological pathways were angiogenesis and T cell-related while wound healing and ECM regulation were downregulated. Pseudotime trajectory analyses revealed that IVD injury directed cells towards increased differentiation in all clusters, except for Krt18 + NP cells which remained in a less mature cell state. Saa2-High and Grem1-High MSCs populations shifted towards more differentiated IVD cells profiles with injury and localized distinctly within the IVD. This study revealed novel MSC populations with the potential to be leveraged for future IVD repair studies.

Cartilage↗

Solid–liquid separation of lignocellulosic sugars from biomass by rotating ceramic disc filtration

In many biomass conversion processes, the separation of cellulosic sugars from residual, lignin-rich solids is a critical step, and achieving high recovery yields of sugars by conventional tangential crossflow and vacuum filtration is challenged by the presence of biomass solids, which rapidly foul filters, resulting in decreased throughput. Considering the performance limitations of traditional filtration methods, dynamic filtration, which generates high shear at the membrane surface to decrease fouling, is emerging as a viable alternative for demanding solid–liquid separations. For high solids separations, there is little available information regarding the performance, limitations, and energy consumption of dynamic filtration. To that end, here we characterized the performance of a dynamic filtration module, specifically a rotating ceramic disc (RCD) filter, for the aseptic recovery of cellulosic sugars from biomass solids following pretreatment and enzymatic hydrolysis. We show how RCD rotational velocity and percent biomass solids impact the filter throughput. Additionally, we used computational fluid dynamics (CFD) simulations to estimate the shear rate at the membrane surface and to visualize hydrodynamic profiles within the module. With the combined CFD simulations and experimental results, we estimated the energy demand and operating expenses for a viable dynamic filtration system operating with a lignocellulosic feed slurry. Our results indicate that an RCD filter can achieve ≥95% recovery of sugars and produce a retentate slurry containing 12 wt% insoluble solids with low energy consumption (a 2.2-fold improvement over cross-flow filtration) and low operating costs ($\$$0.06 per kg sugars). These results show a viable path towards operationally reliable, energy efficient, and cost-effective separations of sterilized cellulosic sugars from biomass solids and highlight the potential of dynamic filtration systems for challenging solid–liquid separations.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗

Influence of Comptonization region over the ambiance of accretion disc in active galactic nucleus

ABSTRACT Understanding the radiative and physical structures of inner region of a few 100 pc of active galactic nucleus (AGNs) is important to constrain the causes of their activities. Although the X-ray emission from the Comptonization region/corona and the accretion disc regulates the broad-line emission regions and torus structures, the exact mutual dependency is not understood well. We performed correlation studies for X-ray, mid-infrared, and different components of Balmer emission lines for the selected sample of AGNs. Almost 10 different parameters and their interdependencies were explored in order to understand the underlying astrophysics. We found that the X-ray luminosity has a linear dependency on the various components of broad Balmer emission lines (e.g. L$_{\text{2-10 keV}}\, \propto$ L$^{0.78}_{\text{H}\beta ^{\text{B}}}$) and found a strong dependency on the optical continuum luminosity (L$_{\text{2-10 keV}}\, \propto$ L$^{0.86}_{5100\, \mathring{\rm A}}$). For a selected sample, we also observed a linear dependency between X-ray and mid-infrared luminosity (L$_{\text{2-10 keV}}\, \propto$ L$^{0.74}_{6\, \mu \text{m}}$). A break point was observed in our correlation studies for X-ray power-law index, Γ, and mass of black hole at ∼ log (M/M⊙) = 8.95. Similarly, the relations between Γ and full width at half-maximum (FWHM) of H α and H β broad components show breaks at FWHMH α = 7642 ± 657 km s−1 and FWHMH β = 7336 ± 650 km s−1. However, more data are required to confine the breaks locations exactly. We noted that Γ and Eddington ratios are negatively correlated to Balmer decrements in our selected sample. We analysed and discussed about the implications of new findings in terms of interaction AGN structures.

79 ASTRONOMY AND ASTROPHYSICS↗

A kinematic excess in the annular gap and gas-depleted cavity in the disc around HD 169142

ABSTRACT We present ALMA band 6 images of the 12CO, 13CO, and C18O J = 2-1 line emissions for the circumstellar disc around HD 169142, at ∼8 au spatial resolution. We resolve a central gas-depleted cavity, along with two independent near-symmetric ring-like structures in line emission: a well-defined inner gas ring [∼25 au] and a second relatively fainter and diffuse outer gas ring [∼65 au]. We identify a localized super-Keplerian feature or vertical flow with a magnitude of ∼75 ms−1 in the 12CO map. This feature has the shape of an arc that spans azimuthally across a position angle range of −60° to 45° and radially in between the B1[26au] and B2[59au] dust rings. Through reconstruction of the gas surface density profile, we find that the magnitude of the background perturbations by the pressure support and self-gravity terms are not significant enough to account for the kinematic excess. If of planetary origin, the relative depletion in the gas-density profile would suggest a 1 MJ planet. In contrast, the central cavity displays relatively smooth kinematics, suggesting either a low-mass companion and/or a binary orbit with a minimal vertical velocity component.

79 ASTRONOMY AND ASTROPHYSICS↗

Secular outflows from 3D MHD hypermassive neutron star accretion disc systems

Magnetized hypermassive neutron stars (HMNSs) have been proposed as a way for neutron star mergers to produce high electron fraction, high-velocity ejecta, as required by kilonova models to explain the observed light curve of GW170817. The HMNS drives outflows through neutrino energy deposition and mechanical oscillations, and raises the electron fraction of outflows through neutrino interactions before collapsing to a black hole (BH). Here, in this study, we perform 3D numerical simulations of HMNS–torus systems in ideal magnetohydrodynamics, using a leakage/absorption scheme for neutrino transport, the nuclear APR equation of state, and Newtonian self-gravity, with a pseudo-Newtonian potential added after BH formation. Due to the uncertainty in the HMNS collapse time, we choose two different parametrized times to induce collapse. We also explore two initial magnetic field geometries in the torus, and evolve the systems until the outflows diminish significantly ($\sim\!\! 1\!\! - \!\!2\ \mathrm{s}$). We find bluer, faster outflows as compared to equivalent BH–torus systems, producing M ∼ 10 −3 M ⊙ of ejecta with Y e ≥ 0.25 and v ≥ 0.25c by the simulation end. Approximately half the outflows are launched in disc winds at times $t\lesssim 500 \ \mathrm{ms}$, with a broad distribution of electron fractions and velocities, depending on the initial condition. The remaining outflows are thermally driven, characterized by lower velocities and electron fractions. Nucleosynthesis with tracer particles shows patterns resembling solar abundances in all models. Although outflows from our simulations do not match those inferred from two-component modelling of the GW170817 kilonova, self-consistent multidimensional detailed kilonova models are required to determine whether our outflows can power the blue kilonova.

79 ASTRONOMY AND ASTROPHYSICS↗

Feedstock/pretreatment screening for bioconversion of sugar and lignin streams via deacetylated disc-refining

Recent publications have shown the benefits of deacetylation disc-refining (DDR) as a pretreatment process to deconstruct biomass into sugars and lignin residues. Major advantages of DDR pretreatment over steam and dilute acid pretreatment are the removal of acetyl and lignin during deacetylation. DDR does not generate hydroxymethylfurfural (HMF) and furfural which are commonly produced from steam and dilute acid pretreatments. Acetate, lignin, HMF, and furfural are known inhibitors during enzymatic hydrolysis and fermentation. Another advantage of deacetylation is the production of lignin-rich black liquor, which can be upgraded to other bioproducts. Furthermore, due to the lack of sugar degradation during deacetylation, DDR has significantly less sugar loss than other pretreatment methods. Previous studies for DDR have primarily focused on corn stover, but lacked the investigative studies of other feedstocks. This study was designed to screen various DDR process conditions at pilot scale using three different feedstocks, including corn stover, poplar, and switchgrass. The impact of the pretreatment conditions was evaluated by testing hydrolysates for bioconversion to 2,3-butanediol. Pretreatment of biomass by DDR showed high-conversion-yields and 2,3-BDO fermentation production yields. Techno-economic analysis (TEA) of the pretreatment for biomass to sugar was also developed based on NREL’s Aspen Model. This study shows that the cellulose and hemicellulose in poplar was more recalcitrant than herbaceous feedstocks which ultimately drove up the sugar cost. Switchgrass was also more recalcitrant than corn stover but less than poplar.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗

Advection in Accretion Discs Boundary Layers

Accretion disc boundary layer models have been calculated for various systems, using a one dimensional time dependent numerical code.

accretion accretion discs stars pre-main-sequence ↗

Irradiation of an Accretion Disc by a Jet: General Properties and Implications for Spin Measurements of Black Holes

X-ray irradiation of the accretion disc leads to strong reflection features, which are then broadened and distorted by relativistic effects. We present a detailed, general relativistic approach to model this irradiation for different geometries of the primary X-ray source. These geometries include the standard point source on the rotational axis as well as more jet-like sources, which are radially elongated and accelerating. Incorporating this code in the RELLINE model for relativistic line emission, the line shape for any configuration can be predicted. We study how different irradiation geometries affect the determination of the spin of the black hole. Broad emission lines are produced only for compact irradiating sources situated close to the black hole. This is the only case where the black hole spin can be unambiguously determined. In all other cases the line shape is narrower, which could either be explained by a low spin or an elongated source. We conclude that for those cases and independent of the quality of the data, no unique solution for the spin exists and therefore only a lower limit of the spin value can be given

Accretion Disc↗

Newly Released GPCP Version 3.2 Global Precipitation Datasets at NASA GES DISC

The Global Precipitation Climatology Project (GPCP) is the precipitation component of an internationally coordinated set of (mainly) satellite-based global products dealing with the Earth's water and energy cycles, under the auspices of the Global Water and Energy Experiment (GEWEX) Data and Assessment Panel (GDAP) of the World Climate Research Program. As the follow-on to the GPCP Version 2.X products, GPCP Version 3 (GPCP V3.2) seeks to continue the production of long, homogeneous precipitation record using modern input and calibration datasets. The GPCP V3.2 provides globally complete analyses of surface precipitation on a 0.5°x 0.5° latitude/longitude grid at both monthly and daily intervals, respectively covering 1983 to the present and June 2000 to the present. New data fields have been introduced to better characterize the precipitation, particularly including an estimate of the fraction of the precipitation that is liquid (rain) in both the Monthly and Daily, and a Quality Index for the Monthly. Compared to the operational GPCP V2.3 Monthly, the V3.2 Monthly provides a more reasonable climatology in the Southern Ocean, and increases the global average precipitation by about 4.46%, which is in line with recommendations of recent assessments. However, the two versions have comparable global and regional trends for 1983-2020. Compared to the operational One-Degree Daily (Version 1.3) product, the V3.2 Daily better represents the histogram of precipitation rates, particularly at high values. In this presentation, we will present the latest GPCP V3.2 daily and monthly datasets archived and distributed at NASA Goddard Earth Sciences (GES) Data and Information Services Center (DISC) along with examples from NASA Giovanni.

Precipitation↗

Use of Frit‐Disc Crucible Sets to Make Solution Growth More Quantitative and Versatile

The recent availability of step‐edge, frit‐disc crucible sets (generally sold as Canfield Crucible Sets or CCS) has led to multiple innovations associated with the group's use of solution growth. The use of CCS allows for the clean separation of liquid from solid phases during the growth process. This clean separation enables the reuse of the decanted liquid, either allowing for simple, economic, savings associated with recycling expensive precursor elements or allowing for the fractionation of a growth into multiple, small steps, revealing the progression of multiple solidifications. Clean separation of liquid from solid phases also allows for the determination of the liquidus line (or surface) and the creation, or correction, of composition–temperature phase diagrams. The reuse of clean decanted liquid has also allowed to prepare liquids ideally suited for the growth of large single crystals of specific phases by tuning the composition of the melt to the optimal composition for growth of the desired phase, often with reduced nucleation sites. Finally, it is discussed how solution growth and CCS use can be harnessed to provide a plethora of composition–temperature data points defining liquidus lines or surfaces with differing degrees of precision to either test or anchor artificial intelligence and/or machine‐learning‐based attempts to augment and extend the limited experimentally determined database.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗

Analysis of multi-electron, multi-step homogeneous catalysis by rotating disc electrode voltammetry: theory, application, and obstacles

Rotating disc electrode (RDE) voltammetry has been widely adopted for the study of heterogenized molecular electrocatalysts for multi-step fuel-forming reactions but this tool has never been comprehensively applied to their homogeneous analogues. Here, the utility and limitations of RDE techniques for mechanistic and kinetic analysis of homogeneous molecular catalysts that mediate multi-electron, multi-substrate redox transformations are explored. Using the ECEC' reaction mechanism as a case study, two theoretical models are derived based on the Nernst diffusion layer model and the Hale transformation. Current–potential curves generated by these computational strategies are compared under a variety of limiting conditions to identify conditions under which the more minimalist Nernst Diffusion Layer approach can be applied. Based on this theoretical treatment, strategies for extracting kinetic information from the plateau current and the foot of the catalytic wave are derived. RDEV is applied to a cobaloxime hydrogen evolution reaction (HER) catalyst under non-aqueous conditions in order to experimentally validate this theoretical framework and explore the feasibility of RDE as a tool for studying homogeneous catalysts. Importantly, analysis of the foot-of-the-wave via this theoretical framework provides rate constants for elementary reaction steps that agree with those extracted from stationary voltammetric methods, supporting the application of RDE to study homogeneous fuel-forming catalysts. Lastly, obstacles encountered during the kinetic analysis of cobaloxime, along with the voltammetric signatures used to diagnose this reactivity, are discussed with the goal of guiding groups working to improve RDE set-ups and help researchers avoid misinterpretation of RDE data.

08 HYDROGEN↗

Diffuse LINER-type emission from extended disc regions of barred galaxies

ABSTRACT We present a spectroscopic analysis of the central disc regions of barred spiral galaxies, concentrating on the region that is swept by the bar but not including the bar itself (the ‘star formation desert’ or SFD region). New spectroscopy is presented for 34 galaxies, and the full sample analysed comprises 48 SBa–SBcd galaxies. These data confirm the full suppression of SF within the SFD regions of all but the latest type (SBcd) galaxies. However, diffuse [N ii] and H α line emission is detected in all galaxies. The ubiquity and homogeneous properties of this emission from SBa to SBc galaxies favour post-asymptotic giant branch (p-AGB) stars as the source of this line excitation, rather than extreme blue horizontal branch stars. The emission-line ratios strongly exclude any contribution from recent SF, but are fully consistent with recent population synthesis modelling of p-AGB emission by other authors, and favour excitation dominated by ambient gas of approximately solar abundance, rather than ejecta from the AGB stars themselves. The line equivalent widths are also larger than those observed in many fully passive (e.g. elliptical) galaxies, which may also be a consequence of a greater ambient gas density in the SFD regions.

Percival, S. M.↗

The chemical nature of the young 120-Myr-old nearby Pisces–Eridanus stellar stream flowing through the Galactic disc

Recently, a new cylindrical-shaped stream of stars up to 700 pc long was discovered hiding in the Galactic disc using kinematic data enabled by the Gaia mission. This stream of stars, dubbed Pisces–Eridanus (Psc–Eri), was initially thought to be as old as 1 Gyr, yet its stars shared a rotation period distribution consistent with a population that was 120 Myr old. Here, we explore the detailed chemical nature of this stellar stream. We carried out high-resolution spectroscopic follow-up of 42 Psc–Eri stars using McDonald Observatory and combined these data with information for 40 members observed with the low-resolution LAMOST spectroscopic survey. Together, these data enabled us to measure the abundance distribution of light/odd-Z (Li, Na, Al, Sc, V), α (Mg, Si, Ca, Ti), Fe-peak (Cr, Mn, Fe, Co, Ni, Zn), and neutron capture (Sr, Y, Zr, Ba, La, Nd, Eu) elements along the Psc–Eri stream. We find that the stream is (1) near-solar metallicity with [Fe/H] = –0.03 dex and (2) has a metallicity spread of 0.07 dex (or 0.04 dex when outliers are excluded). We also find that (3) the abundance of Li indicates that Psc–Eri is ~120 Myr old, consistent with its gyrochronology age. Additionally, Psc–Eri has (4) [X/Fe] abundance spreads that are just larger than the typical uncertainty in most elements, (5) it is a cylindrical-like system whose outer edges rotate about the centre, and (6) no significant abundance gradients along its major axis except a potentially weak gradient in [Si/Fe]. These results show that Psc–Eri is a uniquely close young chemically interesting laboratory for testing our understanding of star and planet formation.

79 ASTRONOMY AND ASTROPHYSICS↗

Strong chemical tagging with APOGEE: 21 candidate star clusters that have dissolved across the Milky Way disc

ABSTRACT Chemically tagging groups of stars born in the same birth cluster is a major goal of spectroscopic surveys. To investigate the feasibility of such strong chemical tagging, we perform a blind chemical tagging experiment on abundances measured from APOGEE survey spectra. We apply a density-based clustering algorithm to the 8D chemical space defined by [Mg/Fe], [Al/Fe], [Si/Fe], [K/Fe], [Ti/Fe], [Mn/Fe], [Fe/H], and [Ni/Fe], abundances ratios which together span multiple nucleosynthetic channels. In a high-quality sample of 182 538 giant stars, we detect 21 candidate clusters with more than 15 members. Our candidate clusters are more chemically homogeneous than a population of non-member stars with similar [Mg/Fe] and [Fe/H], even in abundances not used for tagging. Group members are consistent with having the same age and fall along a single stellar-population track in log g versus Teff space. Each group’s members are distributed over multiple kpc, and the spread in their radial and azimuthal actions increases with age. We qualitatively reproduce this increase using N-body simulations of cluster dissolution in Galactic potentials that include transient winding spiral arms. Observing our candidate birth clusters with high-resolution spectroscopy in other wavebands to investigate their chemical homogeneity in other nucleosynthetic groups will be essential to confirming the efficacy of strong chemical tagging. Our initially spatially compact but now widely dispersed candidate clusters will provide novel limits on chemical evolution and orbital diffusion in the Galactic disc, and constraints on star formation in loosely bound groups.

Price-Jones, Natalie↗

The turbulence driving parameter of molecular clouds in disc galaxies

ABSTRACT Supersonic turbulence plays a pivotal role during the formation of molecular clouds and stars in galaxies. However, little is known about how the fraction of compressive and solenoidal modes in the velocity field evolves over time and how it depends on properties of the molecular cloud or the galactic environment. In this work, we carry out magnetohydrodynamical simulations of disc galaxies and study the time evolution of the turbulence driving parameter for an ensemble of clouds. We find that the time-averaged turbulence driving parameter is insensitive to the position of the cloud within the galaxy. The ensemble-averaged driving parameter is found to be rather compressive with b ∼ 0.5–0.7, indicating almost time-independent global star formation properties. However, each individual cloud shows a highly fluctuating driving parameter, which would strongly affect the cloud’s star formation rate. We find that the mode of turbulence driving can rapidly change within only a few Myr, both from solenoidal to compressive and vice versa. We attribute these changes to cloud collisions and to tidal interactions with clouds or overdensities in the environment. Last, we find no significant differences in the average driving parameter between hydrodynamic and initially strongly magnetized galaxies. However, the magnetic field tends to reduce the overall fluctuation of the driving parameter. The average driving and its uncertainty are seen to be in agreement with recent constraints on the turbulence driving mode for solar neighbourhood clouds.

Körtgen, Bastian↗

Stars in the local galactic thick disc and halo in Gaia EDR3: a catalogue of half a million local main-sequence stars with photometric metallicities

We present a catalogue of 551 214 main-sequence stars in the local (d < 2 kpc) Galactic thick disc and halo, based on a search of stars with large proper motions ($\mu _{\rm tot} \gt 40.0~\rm mas\, yr^{-1}$) in the Gaia Early Data Release 3. We derive photometric metallicity calibrated from the colour–luminosity–metallicity distribution of 20 047 stars with spectroscopic metallicities, collected from various spectroscopic surveys, including SDSS SEGUE/APOGEE, GALAH DR3, and LAMOST DR6. We combine these results to construct an empirical colour–magnitude–metallicity grid, which can be used to estimate photometric metallicities for low-mass metal-poor stars of K and M subtypes from their absolute G magnitude and colour values. We find that low-mass, high-velocity stars in our catalogue share similar kinematics as reported in recent studies of more luminous Galactic halo stars. The pseudo-kinematic analysis of our sample recovers the main local halo structures, including the Gaia–Enceladus Stream and the Helmi stream; aside from these, the local halo stars appear to show a remarkably smooth distribution in velocity space. Since the future Gaia data release will provide radial velocity measurements for only a small number of our sample, our catalogue provides targets of high interest for the future spectroscopic observation programs.

79 ASTRONOMY AND ASTROPHYSICS↗