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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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At least 217 records · Page 12
All sourcesNASA NTRSDOE OSTI
DOE OSTI2025

Experimental Investigation of Air-Fuel Equivalence Ratio Effects on Advanced Dual-fuel Diesel/Ammonia Combustion on a Single-Cylinder Medium-Duty Diesel Engine at High Load

Ammonia (NH3) has gathered a lot of interest as a low-lifecycle-carbon fuel in sectors with high weight and distance requirements, such as shipping. The International Maritime Organization (IMO) mandates a 70-80% greenhouse gas (GHG) reduction by 2040, which is only possible with advanced engine technologies and fuels like NH3. Prior research studies at the US Department of Energy’s Oak Ridge National Laboratory have shown strong performance with NH3 under dual-fuel mode using conventional diesel combustion (CDC) manifold air pressure (MAP) settings. Diesel airflow was initially used to simplify retrofitting (no turbocharger modification), which resulted in air-fuel equivalence ratios (λ) greater than 1.5. To characterize potential improvements in dual-fuel NH3 combustion performance at richer in-cylinder conditions, a global λ sweep using a diesel pilot ignition (DPI) strategy with diesel fuel injected near top-dead center (TDC) and a reactivity-controlled compression ignition (RCCI) injection strategy with diesel fuel injected earlier during the compression stroke were compared. The experiments were conducted at 1200 RPM and 12.6 bar (75% load), and λ was varied by decreasing the commanded air flow to the engine at greater than 90% ammonia energy substitution (AES) level. A diesel injection timing sweep was conducted for both the combustion modes at fixed λ, and the timing with the lowest engine-out N2O emissions was identified. The results indicated an optimal balance between CO2, eq and thermal efficiency benefits for both DPI and RCCI injection strategy cases compared to CDC at a λ of 1.4. The indicated N-based emissions exhibited a strong correlation to the ratio of CA5–50 and ignition delay for DPI, but no apparent trend emerged for the RCCI injection strategy at the tested boundary conditions.

Tyrewala, Daanish [ORNL] (ORCID:0000000208599324)↗
DOE OSTI2023

Cross-correlating radial peculiar velocities and CMB lensing convergence

We study, for the first time, the cross correlation between the angular distribution of radial peculiar velocities (PV) and the lensing convergence of cosmic microwave background (CMB) photons. We derive theoretical expectations for the signal and its covariance and assess its detectability with existing and forthcoming surveys. We find that such cross-correlations are expected to improve constraints on different gravitational models by partially breaking degeneracies with the matter density. We identify in the distance-scaling dispersion of the peculiar velocities the most relevant source of noise in the cross correlation. For this reason, we also study how the above picture changes assuming a redshift-independent scatter for the PV, obtained for example using a reconstruction technique. Our results show that the cross correlation might be detected in the near future combining PV measurements from DESI and the convergence map from CMB-S4. Using realistic direct PV measurements we predict a cumulative signal-to-noise ratio of approximately 3.8σ using data on angular scales 3 ≤ ℓ ≤ 200. For an idealized reconstructed peculiar velocity map extending up to redshift z = 0.15 and a smoothing scale of 4 Mpc h -1 we predict a cumulative signal-to-noise ratio of approximately 27σ from angular scales 3 ≤ ℓ ≤ 200. We conclude that currently reconstructed peculiar velocities have more constraining power than directly observed ones, even though they are more cosmological-model dependent.

79 ASTRONOMY AND ASTROPHYSICS↗
DOE OSTI2025

On Alfvénic turbulence of solar wind streams observed by Solar Orbiter during March 2022 perihelion and their source regions

It has been recently accepted that the standard classification of the solar wind solely according to flow speed is outdated, and particular interest has been devoted to the study of the origin and evolution of so-called Alfvénic slow solar wind streams and to what extent such streams resemble or differ from fast wind. In March 2022, Solar Orbiter completed its first nominal phase perihelion passage. During this interval, it observed several Alfvénic streams, allowing for characterization of fluctuations in three slow wind intervals (AS1-AS3) and comparison with a fast wind stream (F) at almost the same heliocentric distance. This work makes use of Solar Orbiter plasma parameters from the Solar Wind Analyzer (SWA) and magnetic field measurements from the magnetometer (MAG). The magnetic connectivity to the solar sources of selected solar wind intervals was reconstructed using a ballistic extrapolation based on measured solar wind speed down to the (spherical) source surface at 2.5 R s below which a potential field extrapolation was used to map back to the Sun. The source regions were identified using SDO/AIA observations. A spectral analysis of in situ measured magnetic field and velocity fluctuations was performed to characterize correlations, Alfvénicity, normalized cross-helicity, and residual energy in the frequency domain as well as intermittency of the fluctuations and spectral energy transfer rate estimated via mixed third-order moments. A machine learning technique was used to separate proton core, proton beam, and alpha particles and to study v − b correlations for the different ion populations in order to evaluate the role played by each population in determining the Alfvénic content of solar wind fluctuations. The comparison between fast wind and Alfvénic slow wind intervals highlights the differences between the two solar wind regimes: The fast wind is characterized by larger amplitude fluctuations, and magnetic and velocity fluctuations are closer to equipartition of energy. In fact the Alfvénic slow wind streams appear to be on a spectrum of wind types, with AS1, originating from open field lines neighboring active regions and displaying similarities with the fast wind in terms of fluctuation amplitude and turbulence characteristics, but not with respect to the alpha particles and proton beams. The other two slow streams differed both in their sources as well as plasma characteristics, with AS2 coming from the expansion of a narrow coronal hole corridor and AS3 from a region straddling a pseudostreamer. The latter displayed the coldest and highest density but the slowest stream with the smallest fluctuation amplitude and greatest magnetic energy excess. It also showed the largest scatter in proton beam speeds and the greatest difference in speed between proton beam and alpha particles. This study shows how the old fast–slow solar wind dichotomy, already called into question by the observations of slower Alfvénic solar wind streams, should further be refined, as the Alfvénic slow wind, originating in different solar wind regions, show significant differences in density, temperature, and proton and alpha-particle properties in the inner heliosphere. The observations presented here provide the starting point for a better understanding of the origin and evolution of different solar wind streams as well as the evolving turbulence contained within.

magnetohydrodynamics (MHD)↗
DOE OSTI2024

Neural networks for estimation of divertor conditions in DIII-D using C III imaging

Deep learning approaches have been applied to images of C III emission in the lower divertor of DIII-D to develop models for estimating the level of detachment and magnetic configuration (X-point location and strike point radial location). The poloidal distance from the target to the C III emission front is used to represent the level of detachment. The models perform well on a test dataset not used in training, achieving $F_1$ scores as high as 0.99 for detachment state classification and root mean squared error (RMSE) as low as 2cm for front location regression. Predictions for shots with intermittent reattachment are studied, with class activation mapping used to aid in interpretation of the model predictions. Based on the success of these models, a third model was trained to predict the X-point location and strike point radial position from C III images. Though the dataset covers only a small range of possible magnetic configurations, the model shows promising results, achieving RMSE around 1cm for the test data.

70 PLASMA PHYSICS AND FUSION TECHNOLOGY↗
DOE OSTI2021

Redshift evolution of the H2/H i mass ratio in galaxies

ABSTRACT In this paper, we present an attempt to estimate the redshift evolution of the molecular to neutral gas mass ratio within galaxies (at fixed stellar mass). For a sample of five nearby grand design spirals located on the main-sequence (MS) of star-forming galaxies, we exploit maps at 500 pc resolution of stellar mass and star formation rate (M⋆ and SFR). For the same cells, we also have estimates of the neutral (MH i) and molecular ($M_{\rm H_2}$) gas masses. To compute the redshift evolution, we exploit two relations: (i) one between the molecular-to-neutral mass ratio and the total gas mass (Mgas), whose scatter shows a strong dependence with the distance from the spatially resolved MS, and (ii) the one between $\log (M_{\rm {H_2}}/M_{\star })$ and log (MH i/M⋆). For both methods, we and that $M_{\rm H_2}$/MH i within the optical radius slightly decreases with redshift, contrary to common expectations of galaxies becoming progressively more dominated by molecular hydrogen at high redshifts. We discuss possible implications of this trend on our understanding of the internal working of high-redshift galaxies.

Morselli, Laura (ORCID:0000000307532571)↗
DOE OSTI2024

Wind Turbine Water Setbacks: Ordinances (2022) and Extrapolated Trends, 115 Hub Height 170 Rotor Diameter

This dataset represents wind energy setback requirements from water based on existing county ordinances as of April 2022. A setback requirement is a minimum distance from water that an energy project may be developed, and these varied widely across the counties in which they existed. Two versions are provided: one reflecting only the county ordinances and another incorporating extrapolated trends. In the extrapolated version, a median setback equivalent to 1.1 times the turbine tip-height was applied in counties that lacked specific water setback regulations. A TIF data file and a PNG map of the data are provided for both versions, showing areas where wind energy is prohibited or permitted across the contiguous United States. The turbine parameters used were a hub-height of 115 meters and a rotor diameter of 170 meters, as obtained from the Annual Technology Baseline (ATB) 2022. For further details and citation, please refer to the publication linked below: Lopez, Anthony, Pavlo Pinchuk, Michael Gleason, Wesley Cole, Trieu Mai, Travis Williams, Owen Roberts, Marie Rivers, Mike Bannister, Sophie-Min Thomson, Gabe Zuckerman, and Brian Sergi. 2024. Solar Photovoltaics and Land-Based Wind Technical Potential and Supply Curves for the Contiguous United States: 2023 Edition. Golden, CO: National Renewable Energy Laboratory. NREL/TP-6A20-87843.

Array↗
DOE OSTI2022

The Pantheon+ Analysis: Evaluating Peculiar Velocity Corrections in Cosmological Analyses with Nearby Type Ia Supernovae

Separating the components of redshift due to expansion and peculiar motion in the nearby universe (z < 0.1) is critical for using Type Ia Supernovae (SNe Ia) to measure the Hubble constant (H 0 ) and the equation-of-state parameter of dark energy (w). Here, we study the two dominant "motions" contributing to nearby peculiar velocities: large-scale, coherent-flow (CF) motions and small-scale motions due to gravitationally associated galaxies deemed to be in a galaxy group. We use a set of 584 low-z SNe from the Pantheon+ sample, and evaluate the efficacy of corrections to these motions by measuring the improvement of SN distance residuals. We study multiple methods for modeling the large and small-scale motions and show that, while group assignments and CF corrections individually contribute to small improvements in Hubble residual scatter, the greatest improvement comes from the combination of the two (relative standard deviation of the Hubble residuals, Rel. SD, improves from 0.167 to 0.157 mag). We find the optimal flow corrections derived from various local density maps significantly reduce Hubble residuals while raising H 0 by ~0.4 km s -1 Mpc -1 as compared to using CMB redshifts, disfavoring the hypothesis that unrecognized local structure could resolve the Hubble tension. We estimate that the systematic uncertainties in cosmological parameters after optimally correcting redshifts are 0.06–0.11 km s -1 Mpc -1 in H 0 and 0.02–0.03 in w which are smaller than the statistical uncertainties for these measurements: 1.5 km s -1 Mpc -1 for H 0 and 0.04 for w.

79 ASTRONOMY AND ASTROPHYSICS↗
DOE OSTI2024

Wind Turbine Transmission Setbacks: Ordinances (2022) and Extrapolated Trends, 115 Hub Height 170 Rotor Diameter

This dataset represents wind energy setback requirements from transmission based on existing county ordinances as of April 2022. A setback requirement is a minimum distance from transmission infrastructure that an energy project may be developed, and these varied widely across the counties in which they existed. Two versions are provided: one reflecting only the county ordinances and another incorporating extrapolated trends. In the extrapolated version, a median setback equivalent to 1.1 times the turbine tip-height was applied in counties that lacked specific transmission setback regulations. A TIF data file and a PNG map of the data are provided for both versions, showing areas where wind energy is prohibited or permitted across the contiguous United States. The turbine parameters used were a hub-height of 115 meters and a rotor diameter of 170 meters, as obtained from the Annual Technology Baseline (ATB) 2022. For further details and citation, please refer to the publication linked below: Lopez, Anthony, Pavlo Pinchuk, Michael Gleason, Wesley Cole, Trieu Mai, Travis Williams, Owen Roberts, Marie Rivers, Mike Bannister, Sophie-Min Thomson, Gabe Zuckerman, and Brian Sergi. 2024. Solar Photovoltaics and Land-Based Wind Technical Potential and Supply Curves for the Contiguous United States: 2023 Edition. Golden, CO: National Renewable Energy Laboratory. NREL/TP-6A20-87843.

Array↗
DOE OSTI2024

Wind Turbine Structure Setbacks: Ordinances (2022) and Extrapolated Trends, 115 Hub Height 170 Rotor Diameter

This dataset represents wind energy setback requirements from structures based on existing county ordinances as of April 2022. A setback requirement is a minimum distance from a structure that an energy project may be developed, and these varied widely across the counties in which they existed. Two versions are provided: one reflecting only the county ordinances and another incorporating extrapolated trends. In the extrapolated version, a median setback equivalent to 2 times the turbine tip-height was applied in counties that lacked specific structure setback regulations. A TIF data file and a PNG map of the data are provided for both versions, showing areas where wind energy is prohibited or permitted across the contiguous United States. The turbine parameters used were a hub-height of 115 meters and a rotor diameter of 170 meters, as obtained from the Annual Technology Baseline (ATB) 2022. For further details and citation, please refer to the publication linked below: Lopez, Anthony, Pavlo Pinchuk, Michael Gleason, Wesley Cole, Trieu Mai, Travis Williams, Owen Roberts, Marie Rivers, Mike Bannister, Sophie-Min Thomson, Gabe Zuckerman, and Brian Sergi. 2024. Solar Photovoltaics and Land-Based Wind Technical Potential and Supply Curves for the Contiguous United States: 2023 Edition. Golden, CO: National Renewable Energy Laboratory. NREL/TP-6A20-87843.

Array↗
DOE OSTI2024

Wind Turbine Road Setbacks: Ordinances (2022) and Extrapolated Trends, 115 Hub Height 170 Rotor Diameter

This dataset represents wind energy setback requirements from roads based on existing county ordinances as of April 2022. A setback requirement is a minimum distance from a road that an energy project may be developed, and these varied widely across the counties in which they existed. Two versions are provided: one reflecting only the county ordinances and another incorporating extrapolated trends. In the extrapolated version, a median setback equivalent to 1.1 times the turbine tip-height was applied in counties that lacked specific railroad setback regulations. A TIF data file and a PNG map of the data are provided for both versions, showing areas where wind energy is prohibited or permitted across the contiguous United States. The turbine parameters used were a hub-height of 115 meters and a rotor diameter of 170 meters, as obtained from the Annual Technology Baseline (ATB) 2022. For further details and citation, please refer to the publication linked below: Lopez, Anthony, Pavlo Pinchuk, Michael Gleason, Wesley Cole, Trieu Mai, Travis Williams, Owen Roberts, Marie Rivers, Mike Bannister, Sophie-Min Thomson, Gabe Zuckerman, and Brian Sergi. 2024. Solar Photovoltaics and Land-Based Wind Technical Potential and Supply Curves for the Contiguous United States: 2023 Edition. Golden, CO: National Renewable Energy Laboratory. NREL/TP-6A20-87843.

Array↗
DOE OSTI2024

Wind Turbine Railroad Setbacks: Ordinances (2022) and Extrapolated Trends, 115 Hub Height 170 Rotor Diameter

This dataset represents wind energy setback requirements from railroad based on existing county ordinances as of April 2022. A setback requirement is a minimum distance from a railroad that an energy project may be developed, and these varied widely across the counties in which they existed. Two versions are provided: one reflecting only the county ordinances and another incorporating extrapolated trends. In the extrapolated version, a median setback equivalent to 1.1 times the turbine tip-height was applied in counties that lacked specific railroad setback regulations. A TIF data file and a PNG map of the data are provided for both versions, showing areas where wind energy is prohibited or permitted across the contiguous United States. The turbine parameters used were a hub-height of 115 meters and a rotor diameter of 170 meters, as obtained from the Annual Technology Baseline (ATB) 2022. For further details and citation, please refer to the publication linked below: Lopez, Anthony, Pavlo Pinchuk, Michael Gleason, Wesley Cole, Trieu Mai, Travis Williams, Owen Roberts, Marie Rivers, Mike Bannister, Sophie-Min Thomson, Gabe Zuckerman, and Brian Sergi. 2024. Solar Photovoltaics and Land-Based Wind Technical Potential and Supply Curves for the Contiguous United States: 2023 Edition. Golden, CO: National Renewable Energy Laboratory. NREL/TP-6A20-87843.

Array↗
DOE OSTI2022

Revealing latent pole and zone line information in atom probe detector maps using crystallographically correlated metrics

Poles and zone lines observed within atom probe field evaporation images are useful for a range of atom probe crystallography studies, including calibration of the reconstruction and crystallographic characterisation of microstructural features such as grain boundaries. However, this information is not always readily apparent. Techniques for plotting crystallographically correlated metrics contained within atom probe data to enhance pole and zone line contrast across the detector space are developed. This includes consideration of the electric field, molecular ions, lattice structure retained within the reconstruction, specific elemental species, the number of pulses between detection events, and the lateral distance between sequential detection events. These approaches are then applied to experimental atom probe tomography datasets on technically pure Al, nanocrystalline Al, highly doped Si, and additively manufactured Inconel 738, Haynes 282, and Ti–6Al–4V. The results facilitate the extension of atom probe crystallography studies to a broader range of crystalline datasets where crystallographic information is not readily apparent from existing methods, as well as a deeper understanding of field evaporation behaviour during an atom probe experiment.

47 OTHER INSTRUMENTATION↗
DOE OSTI2020

Deconstructing laws of accessibility and facility distribution in cities

The era of the automobile has seriously degraded the quality of urban life through costly travel and visible environmental effects. A new urban planning paradigm must be at the heart of our road map for the years to come, the one where, within minutes, inhabitants can access their basic living needs by bike or by foot. In this work, we present novel insights of the interplay between the distributions of facilities and population that maximize accessibility over the existing road networks. Results in six cities reveal that travel costs could be reduced in half through redistributing facilities. In the optimal scenario, the average travel distance can be modeled as a functional form of the number of facilities and the population density. As an application of this finding, it is possible to estimate the number of facilities needed for reaching a desired average travel distance given the population distribution in a city.

42 ENGINEERING↗
DOE OSTI2026

Spatiotemporal Dynamics of the Relative Abundance of Soil Nutrient‐Degrading Enzyme‐Encoding Genes Across Continental US Ecoregions

Understanding the spatiotemporal patterns in the relative abundance of soil extracellular enzyme‐encoding genes is critical for predicting microbial responses to environmental change and their potential role in nutrient cycling. Yet, integrating novel metagenomic observations with spatiotemporal environmental gradients to infer regional patterns and future trajectories has remained unclear. To address this gap, we applied a machine learning (ML) approach, integrating soil metagenomic data with environmental variables—soil properties, topography, vegetation, and climate—to predict the relative abundance of enzyme‐encoding genes for soil carbon (C), nitrogen (N), and phosphorus (P) across surface soils of the continental United States. We assessed potential responses under future emission scenarios (SSP2‐4.5 and SSP5‐8.5) by comparing a baseline (1985–2014) to a future period (2071–2100). The ML model explained 57%–63% of baseline variation. Precipitation was identified as the most influential factor for the relative abundance of C‐ and N‐degrading enzyme‐encoding genes, while slope length, representing horizontal distance that water can travel downslope, was the primary driver for P‐degrading enzyme‐encoding genes abundance. Projections revealed spatially heterogeneous shifts across continental US ecoregions: the relative abundance of C‐ and N‐degrading enzyme‐encoding genes decreased in wetter ecoregions and increased in drier ecoregions under future climate, while P‐degrading enzyme‐encoding genes abundance decreased significantly in semiarid and Mediterranean ecoregions. This study demonstrates the utility of metagenomic data for mapping soil genetic potential and predicting its regional response to environmental change, to inform ecosystem management strategies.

extracellular enzyme-encoding genes↗
DOE OSTI2025

A map of the rubisco biochemical landscape

Rubisco is the primary CO 2 -fixing enzyme of the biosphere, yet it has slow kinetics. The roles of evolution and chemical mechanism in constraining its biochemical function remain debated. Engineering efforts aimed at adjusting the biochemical parameters of rubisco have largely failed, although recent results indicate that the functional potential of rubisco has a wider scope than previously known. Here we developed a massively parallel assay, using an engineered Escherichia coli in which enzyme activity is coupled to growth, to systematically map the sequence–function landscape of rubisco. Composite assay of more than 99% of single-amino acid mutants versus CO 2 concentration enabled inference of enzyme velocity and apparent CO 2 affinity parameters for thousands of substitutions. This approach identified many highly conserved positions that tolerate mutation and rare mutations that improve CO 2 affinity. These data indicate that non-trivial biochemical changes are readily accessible and that the functional distance between rubiscos from diverse organisms can be traversed, laying the groundwork for further enzyme engineering efforts.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗
DOE OSTI2025

Roughrider Carbon Storage Hub (Final Report)

The Roughrider Carbon Storage Hub was a 2-year project (October 2023 – September 2025) conducted by the Energy & Environmental Research Center (EERC) focused on advancing the feasibility of a commercial-scale carbon dioxide (CO 2 ) geologic storage hub in McKenzie County, North Dakota. The project’s objective was to investigate the potential that stacked storage complexes (multiple deep saline formations) can safely and economically store at least 50 million tonnes of CO 2 within 30 years. The captured CO 2 would be sourced from industrial emitters including project partner ONEOK, Inc.’s gas-processing plants and a planned gas-to-liquids facility. Drilling of the Roughrider 1 stratigraphic test well (14,979-ft total depth) was completed in November 2024. The wellbore intersected four candidate storage formations: Inyan Kara, Broom Creek, Mission Canyon, and Black Island–Deadwood. Operational challenges, including a stuck drill string, were resolved without long-term impact. A comprehensive logging and coring program was conducted, followed by successful well abandonment and site reclamation. Over 660 ft of 4-in. whole core was retrieved. Core plug samples were processed and analyzed for petrophysical and geochemical properties. Results confirmed promising porosity and permeability in the Inyan Kara and Broom Creek Formations and removal of the Mission Canyon and Black Island–Deadwood horizons from further investigation. Data derived from the logging and coring program were used to improve initial geologic models built from legacy data. CO 2 injection simulations showed that the Inyan Kara alone can feasibly store the target mass of CO 2 . Because of subtle differences in geologic structure and porosity trends between the formations, a stacked storage scenario using the Broom Creek and Inyan Kara Formations resulted in a larger overall plume area than using the Inyan Kara alone. Preliminary CO 2 pipeline routes from the industrial sources were mapped utilizing existing rights of way and evaluated for capacity and cost using U.S. Department of Energy Office of Fossil Energy and Carbon Management/National Energy Technology Laboratory models and U.S. Environmental Protection Agency emissions data. Integrating capture, transport, and storage cost estimates with policy incentives (e.g., 45Q credits) provided a total cost-per-ton analysis. Results indicate that the small scale of the volumes to be transported over the cumulative large distances does not support the project’s financial viability. However, the groundwork laid during this project from geological, regulatory, and social perspectives positions the Roughrider hub site as a promising candidate for commercial carbon storage in North Dakota, especially if the economy of scale is introduced for CO 2 transportation to the hub site.

01 COAL, LIGNITE, AND PEAT↗
DOE OSTI2023

In Situ Raman Mapping of Si Island Electrodes and Stress Modeling as a Function of Lithiation and Size

Si is known for cracking and delamination during electrochemical cycling of a battery due to the large volume change associated with Li insertion and extraction. However, it has been found experimentally that patterned Si island electrodes that are 200 nm thick and less than 7 μm wide can deform in a purely elastic manner. Inspired by this, we performed in situ Raman stress characterization of model poly-crystalline Si island electrodes using an electrochemical cell coupled with an immersion objective lens and designed for a short working distance. A 5 μm wide Si island electrode showed a parabolic stress profile during lithiation, while for a 15 μm Si island electrode, a stress plateau in the center of the electrode was observed. A continuum model with coupled electro-chemo-mechanical (ECM) physics was established to understand the stress measurement. A qualitative agreement was reached between modeling and experimental data, and the critical size effect could be explained by the Li diffusive flux as governed by competition between the Li concentration and hydrostatic stress gradients. Below the critical size, the stress gradient drives Li toward the edges, where the electrode volume is free to expand, while above the critical size, the stress plateau inhibits Li diffusion to the edge and forces destructive stress relief by cracking. Furthermore, this work represents a promising methodology for in situ characterization of ECM coupling in battery electrodes, with suggestions provided for further improvement.

25 ENERGY STORAGE↗
DOE OSTI2022

Protoplast fusion in Bacillus species produces frequent, unbiased, genome-wide homologous recombination

Abstract In eukaryotes, fine-scale maps of meiotic recombination events have greatly advanced our understanding of the factors that affect genomic variation patterns and evolution of traits. However, in bacteria that lack natural systems for sexual reproduction, unbiased characterization of recombination landscapes has remained challenging due to variable rates of genetic exchange and influence of natural selection. Here, to overcome these limitations and to gain a genome-wide view on recombination, we crossed Bacillus strains with different genetic distances using protoplast fusion. The offspring displayed complex inheritance patterns with one of the parents consistently contributing the major part of the chromosome backbone and multiple unselected fragments originating from the second parent. Our results demonstrate that this bias was in part due to the action of restriction–modification systems, whereas genome features like GC content and local nucleotide identity did not affect distribution of recombination events around the chromosome. Furthermore, we found that recombination occurred uniformly across the genome without concentration into hotspots. Notably, our results show that species-level genetic distance did not affect genome-wide recombination. This study provides a new insight into the dynamics of recombination in bacteria and a platform for studying recombination patterns in diverse bacterial species.

59 BASIC BIOLOGICAL SCIENCES↗