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NASA NTRS1997

Mechanotransduction in bone: osteoblasts are more responsive to fluid forces than mechanical strain

Mechanical force applied to bone produces two localized mechanical signals on the cell: deformation of the extracellular matrix (substrate strain) and extracellular fluid flow. To study the effects of these stimuli on osteoblasts, MC3T3-E1 cells were grown on type I collagen-coated plastic plates and subjected to four-point bending. This technique produces uniform levels of physiological strain and fluid forces on the cells. Each of these parameters can be varied independently. Osteopontin (OPN) mRNA expression was used to assess the anabolic response of MC3T3-E1 cells. When fluid forces were low, neither strain magnitude nor strain rate was correlated with OPN expression. However, higher-magnitude fluid forces significantly increased OPN message levels independently of the strain magnitude or rate. These data indicate that fluid forces, and not mechanical stretch, influence OPN expression in osteoblasts and suggest that fluid forces induced by extracellular fluid flow within the bone matrix may play an important role in bone formation in response to mechanical loading.

Non-NASA Center↗

NASA NTRS1994

A comprehensive Guyton model analysis of physiologic responses to preadapting the blood volume as a countermeasure to fluid shifts

The Guyton model of fluid, electrolyte, and circulatory regulation is an extensive mathematical model capable of simulating a variety of experimental conditions. It has been modified for use at NASA to simulate head-down tilt, a frequently used analog of weightlessness. Weightlessness causes a headward shift of body fluids that is believed to expand central blood volume, triggering a series of physiologic responses resulting in large losses of body fluids. We used the modified Guyton model to test the hypothesis that preadaptation of the blood volume before weightless exposure could counteract the central volume expansion caused by fluid shifts, and thereby attenuate the circulatory and renal responses that result in body fluid losses. Simulation results show that circulatory preadaptation, by a procedure resembling blood donation immediately before head-down bedrest, is effective in damping the physiologic responses to fluid shifts and reducing body fluid losses. After 10 hours of head-down tilt, preadaptation also produces higher blood volume, extracellular volume, and total body water for 20 to 30 days of bedrest, compared with non-preadapted control. These results indicate that circulatory preadaptation before current Space Shuttle missions may be beneficial for the maintenance of reentry and postflight orthostatic tolerance in astronauts. This paper presents a comprehensive examination of the simulation results pertaining to changes in relevant physiologic variables produced by blood volume reduction before a prolonged head-down tilt. The objectives were to study and develop the countermeasure theoretically, to aid in planning experimental studies of the countermeasure, and to identify potentially disadvantageous physiologic responses that may be caused by the countermeasure.

NASA Center JSC↗

NASA NTRS1994

A fiber matrix model for fluid flow and streaming potentials in the canaliculi of an osteon

A theoretical model is developed to predict the fluid shear stress and streaming potential at the surface of osteocytic processes in the lacunar-canalicular porosity of an osteon when the osteon is subject to mechanical loads that are parallel or perpendicular to its axis. The theory developed in Weinbaum et al. (31) for the flow through a proteoglycan matrix in a canaliculus is employed in a poroelastic model for the osteon. Our formulation is a generalization of that of Petrov et al. (17). Our model predicts that, in order to satisfy the measured frequency dependence of the phase and magnitude of the SGP in macroscopic bone samples, the fiber spacing in the fluid annulus must lie in the narrow range 6-7 nm typical of the spacing of GAG sidechains along a protein monomer. The model predictions for the local SGP profiles in the osteon agree with the experimental observations of Starkebaum et al. (24). The theory predicts that the pore pressure relaxation time, tau d, for a 150-300 microns diameter osteon with the foregoing matrix structure is approximately 0.03-0.13 sec, and that the amplitude of the mean fluid shear stress on the membrane of the osteocytic process at the mean areal radius of the osteon has a maximum at 28 Hz if tau d = 0.06 sec. This maximum, which is independent of the magnitude of the loading, could be important in vivo since the recent experiments of Turner et al. (28) and McLeod et al. (15) have a peak in the strain frequency spectrum between 20 and 30 Hz that also appears to be independent of the type (magnitude) of loading. Numerical predictions for the amplitude of the average fluid shear stress on the osteocytic membrane at the mean areal radius of the osteon show that the fluid shear stress associated with the low amplitude 20-30 Hz spectral strain component is at least as large as the average fluid shear stress associated with the high amplitude 1 Hz stride component, although the latter loading is an order of magnitude larger, and has a magnitude that lies within the middle of the range, 6-30 dynes/cm2, where fluid shear stresses in tissue culture studies with osteoblast monolayers have elicited an intracellular Ca++ response (31). The implications of these results for intracellular electrical communication are discussed.

Non-NASA Center↗

NASA NTRS2005

Thermo-Physical Properties of Intermediate Temperature Heat Pipe Fluids

Heat pipes are among the most promising technologies for space radiator systems. The paper reports further evaluation of potential heat pipe fluids in the intermediate temperature range of 400 to 700 K in continuation of two recent reports. More thermo-physical property data are examined. Organic, inorganic, and elemental substances are considered. The evaluation of surface tension and other fluid properties are examined. Halides are evaluated as potential heat pipe fluids. Reliable data are not available for all fluids and further database development is necessary. Many of the fluids considered are promising candidates as heat pipe fluids. Water is promising as a heat pipe fluid up to 500 to 550 K. Life test data for thermo-chemical compatibility are almost non-existent.

Beach, Duane E.↗

NASA NTRS1996

Interface Configuration Experiments (ICE) Explore the Effects of Microgravity on Fluids

The Interface Configuration Experiment (ICE) is actually a series of experiments that explore the striking behavior of liquid-vapor interfaces (i.e., fluid surfaces) in a low gravity environment under which major shifts in liquid position can arise from small changes in container shape or contact angle. Although these experiments are designed to test current mathematical theory, there are numerous practical applications that could result from these studies. When designing fluid management systems for space-based operations, it is important to be able to predict the locations and configurations that fluids will assume in containers under low-gravity conditions. The increased ability to predict, and hence control, fluid interfaces is vital to systems and/or processes where capillary forces play a significant role both in space and on the Earth. Some of these applications are in general coating processes (paints, pesticides, printing, etc.), fluid transport in porous media (ground water flows, oil recovery, etc.), liquid propellant systems in space (liquid fuel and oxygen), capillary-pumped loops and heat pipes, and space-based life-support systems. In space, almost every fluid system is affected, if not dominated, by capillarity. Knowledge of the liquid-vapor interface behavior, and in particular the interface shape from which any analysis must begin, is required as a foundation to predict how these fluids will react in microgravity and on Earth. With such knowledge, system designs can be optimized, thereby decreasing costs and complexity, while increasing performance and reliability. ICE has increased, and will continue to increase this knowledge, as it probes the specific peculiarities of current theory upon which our current understanding of these effects is based. Several versions of ICE were conducted in NASA Lewis Research Center's drop towers and on the space shuttle during the first and second United States Microgravity Laboratory missions (USML-1 and USML-2). Additional tests are planned for the space shuttle and for the Russian Mir space station. These studies will focus on interfacial problems concerning surface existence, uniqueness, configuration, stability, and flow characteristics.

Source record↗

NASA NTRS2004

Thermo-Physical Properties of Intermediate Temperature Heat Pipe Fluids

Heat pipes are among the most promising technologies for space radiator systems. The paper reports further evaluation of potential heat pipe fluids in the intermediate temperature range of 400 to 700 K in continuation of two recent reports. More thermo-physical property data are examined. Organic, inorganic and elemental substances are considered. The evaluation of surface tension and other fluid properties are examined. Halides are evaluated as potential heat pipe fluids. Reliable data are not available for all fluids and further database development in necessary. Many of the fluids considered are promising candidates as heat pipe fluids. Water is promising as a heat pipe fluid up to 500-550 K. Life test data for thermo-chemical compatibility are almost non-existent.

Devarakonda, Angirasa↗

NASA NTRS1992

Method of delivering lunar generated fluid to earth orbit using an external tank

A method and apparatus are provided for delivering lunar generated fluid to Earth orbit from lunar orbit. Transport takes place in an external tank of a shuttle which has been suitably outfitted in Earth orbit for reusable travel between Earth orbit and a lunar orbit. The outfitting of the external tank includes the adding of an engine, an electrical system, a communication system, a guidance system, an aerobraking device, and a plurality of interconnected fluid storage tanks to the hydrogen and oxygen tanks of the external tank. The external tank is then propelled to lunar orbit the first time using Earth-based propellant. In lunar orbit, the storage tanks are filled with the lunar generated fluid with the remainder tank volumes filled with lunar generated liquid oxygen and hydrogen which serve as propellants for returning the tank to Earth orbit where the fluid is off-loaded. The remaining lunar generated oxygen and hydrogen is then sufficient to return the external tank to lunar orbit so that a subsequent cycle of fluid delivery is repeated. A space station in a higher Earth orbit is preferably used to outfit the external tank, and a lunar node in lunar orbit is used to store and transfer the fluid and liquid oxygen and hydrogen to the external tank. The lunar generated fluid is preferably .sup.3 He.

Butterfield, Ansel J.↗

NASA NTRS2009

An Integrated Solution for Performing Thermo-fluid Conjugate Analysis

A method has been developed which integrates a fluid flow analyzer and a thermal analyzer to produce both steady state and transient results of 1-D, 2-D, and 3-D analysis models. The Generalized Fluid System Simulation Program (GFSSP) is a one dimensional, general purpose fluid analysis code which computes pressures and flow distributions in complex fluid networks. The MSC Systems Improved Numerical Differencing Analyzer (MSC.SINDA) is a one dimensional general purpose thermal analyzer that solves network representations of thermal systems. Both GFSSP and MSC.SINDA have graphical user interfaces which are used to build the respective model and prepare it for analysis. The SINDA/GFSSP Conjugate Integrator (SGCI) is a formbase graphical integration program used to set input parameters for the conjugate analyses and run the models. The contents of this paper describes SGCI and its thermo-fluids conjugate analysis techniques and capabilities by presenting results from some example models including the cryogenic chill down of a copper pipe, a bar between two walls in a fluid stream, and a solid plate creating a phase change in a flowing fluid.

Kornberg, Oren↗

NASA NTRS2010

Constant-Differential-Pressure Two-Fluid Accumulator

A two-fluid accumulator has been designed, built, and demonstrated to provide an acceptably close approximation to constant differential static pressure between two fluids over the full ranges of (1) accumulator stroke, (2) rates of flow of the fluids, and (3) common static pressure applied to the fluids. Prior differential- pressure two-fluid accumulators are generally not capable of maintaining acceptably close approximations to constant differential pressures. The inadequacies of a typical prior differential-pressure two-fluid accumulator can be summarized as follows: The static differential pressure is governed by the intrinsic spring rate (essentially, the stiffness) of an accumulator tank. The spring rate can be tailored through selection of the tank-wall thickness, selection of the number and/or shape of accumulator convolutions, and/or selection of accumulator material(s). Reliance on the intrinsic spring rate of the tank results in three severe limitations: (1) The spring rate and the expulsion efficiency tend to be inversely proportional to each other: that is to say, as the stiffness (and thus the differential pressure) is increased, the range of motion of the accumulator is reduced. (2) As the applied common static pressure increases, the differential pressure tends to decrease. An additional disadvantage, which may or may not be considered limiting, depending on the specific application, is that an increase in stiffness entails an increase in weight. (3) The additional weight required by a low expulsion efficiency accumulator eliminates the advantage given to such gas storage systems. The high expulsion efficiency provided by this two-fluid accumulator allows for a lightweight, tightly packaged system, which can be used in conjunction with a fuel cell-based system.

Piecuch, Benjamin↗

NASA NTRS2004

Strain-Gauge Measurement of Weight of Fluid in a Tank

A method of determining the amount of fluid in a tank is based on measurement of strains induced in tank supports by the weight of the fluid. Unlike most prior methods, this method is nonintrusive: there is no need to insert instrumentation in the tank and, hence, no need to run wires, cables, or tubes through the tank wall. Also unlike most prior methods, this method is applicable even if the fluid in the tank is at supercritical pressure and temperature, because it does not depend on the presence of a liquid/gas interface (as in liquid-level-measuring methods). The strain gauges used in this method may be of two types: foil and fiber-optic. Four foil gauges (full bridge) are mounted on each of the tank-supporting legs. As the tank is filled or emptied, the deformation in each leg increases or decreases, respectively. Measured deformations of all legs are added to obtain a composite deformation indicative of the change in weight of the tank plus fluid. An initial calibration is performed by recording data at two points (usually, empty and full) for which the mass or weight of fluid is known. It is assumed that the deformations are elastic, so that the line passing through the two points can be used as a calibration curve of mass (or weight) of fluid versus deformation. One or more fiber-optic gauges may be used instead of the foil gauges. The resolution of the fiber-optic and foil gauges is approximately the same, but the fiber-optic gauges are immune to EMI (electromagnetic interference), are linear with respect to temperature over their entire dynamic range (as defined by the behavior of the sample), and measure thermally induced deformations as predictable signals. Conversely, long term testing has demonstrated that the foil gauges exhibit an erratic behavior whenever subjected to direct sun radiation (even if protected with a rubberized cover). Henceforth, for deployment in outdoor conditions, fiber-optic gauges are the only option if one is to rely on the system for an extended period of time when a recalibration procedure may not be acceptable. A set of foil gauges had been tested on the supports of a 500-gallon (1,900-liter) tank. The gauges were found to be capable of measuring the deformations (up to 22 micro-strain) that occurred during filling and emptying of the tank. The fluid masses calculated from the gauge readings were found to be accurate within 4.5 percent. However, the reliability of the foil gauges over a few hours was not acceptable. Therefore, the foil sensor system is acceptable for use only in controlled environments (complete shade, or indoors).

Figueroa, Jorge↗

NASA NTRS2011

Conjugate Compressible Fluid Flow and Heat Transfer in Ducts

A computational approach to modeling transient, compressible fluid flow with heat transfer in long, narrow ducts is presented. The primary application of the model is for analyzing fluid flow and heat transfer in solid propellant rocket motor nozzle joints during motor start-up, but the approach is relevant to a wide range of analyses involving rapid pressurization and filling of ducts. Fluid flow is modeled through solution of the spatially one-dimensional, transient Euler equations. Source terms are included in the governing equations to account for the effects of wall friction and heat transfer. The equation solver is fully-implicit, thus providing greater flexibility than an explicit solver. This approach allows for resolution of pressure wave effects on the flow as well as for fast calculation of the steady-state solution when a quasi-steady approach is sufficient. Solution of the one-dimensional Euler equations with source terms significantly reduces computational run times compared to general purpose computational fluid dynamics packages solving the Navier-Stokes equations with resolved boundary layers. In addition, conjugate heat transfer is more readily implemented using the approach described in this paper than with most general purpose computational fluid dynamics packages. The compressible flow code has been integrated with a transient heat transfer solver to analyze heat transfer between the fluid and surrounding structure. Conjugate fluid flow and heat transfer solutions are presented. The author is unaware of any previous work available in the open literature which uses the same approach described in this paper.

Cross, M. F.↗

NASA NTRS2013

Intravenous Fluid Generation System

The ability to stabilize and treat patients on exploration missions will depend on access to needed consumables. Intravenous (IV) fluids have been identified as required consumables. A review of the Space Medicine Exploration Medical Condition List (SMEMCL) lists over 400 medical conditions that could present and require treatment during ISS missions. The Intravenous Fluid Generation System (IVGEN) technology provides the scalable capability to generate IV fluids from indigenous water supplies. It meets USP (U.S. Pharmacopeia) standards. This capability was performed using potable water from the ISS; water from more extreme environments would need preconditioning. The key advantage is the ability to filter mass and volume, providing the equivalent amount of IV fluid: this is critical for remote operations or resource- poor environments. The IVGEN technology purifies drinking water, mixes it with salt, and transfers it to a suitable bag to deliver a sterile normal saline solution. Operational constraints such as mass limitations and lack of refrigeration may limit the type and volume of such fluids that can be carried onboard the spacecraft. In addition, most medical fluids have a shelf life that is shorter than some mission durations. Consequently, the objective of the IVGEN experiment was to develop, design, and validate the necessary methodology to purify spacecraft potable water into a normal saline solution, thus reducing the amount of IV fluids that are included in the launch manifest. As currently conceived, an IVGEN system for a space exploration mission would consist of an accumulator, a purifier, a mixing assembly, a salt bag, and a sterile bag. The accumulator is used to transfer a measured amount of drinking water from the spacecraft to the purifier. The purifier uses filters to separate any air bubbles that may have gotten trapped during the drinking water transfer from flowing through a high-quality deionizing cartridge that removes the impurities in the water before entering the salt bag and mixing with the salt to create a normal saline solution.

McQuillen, John↗

NASA NTRS2013

Generalized Fluid System Simulation Program, Version 6.0

The Generalized Fluid System Simulation Program (GFSSP) is a finite-volume based general-purpose computer program for analyzing steady state and time-dependant flow rates, pressures, temperatures, and concentrations in a complex flow network. The program is capable of modeling real fluids with phase changes, compressibility, mixture thermodynamics, conjugate heat transfer between solid and fluid, fluid transients, pumps, compressors and external body forces such as gravity and centrifugal. The thermo-fluid system to be analyzed is discretized into nodes, branches, and conductors. The scalar properties such as pressure, temperature, and concentrations are calculated at nodes. Mass flow rates and heat transfer rates are computed in branches and conductors. The graphical user interface allows users to build their models using the 'point, drag, and click' method; the users can also run their models and post-process the results in the same environment. The integrated fluid library supplies thermodynamic and thermo-physical properties of 36 fluids, and 24 different resistance/source options are provided for modeling momentum sources or sinks in the branches. This Technical Memorandum illustrates the application and verification of the code through 25 demonstrated example problems.

Majumdar, A. K.↗

NASA NTRS2013

Aerodynamic Characterization of a Thin, High-Performance Airfoil for Use in Ground Fluids Testing

The FAA has worked with Transport Canada and others to develop allowance times for aircraft operating in ice-pellet precipitation. Wind-tunnel testing has been carried out to better understand the flowoff characteristics and resulting aerodynamic effects of anti-icing fluids contaminated with ice pellets using a thin, high-performance wing section at the National Research Council of Canada Propulsion and Icing Wind Tunnel. The objective of this paper is to characterize the aerodynamic behavior of this wing section in order to better understand the adverse aerodynamic effects of anti-icing fluids and ice-pellet contamination. Aerodynamic performance data, boundary-layer surveys and flow visualization were conducted at a Reynolds number of approximately 6.0×10(exp 6) and a Mach number of 0.12. The clean, baseline model exhibited leading-edge stall characteristics including a leading-edge laminar separation bubble and minimal or no separation on the trailing edge of the main element or flap. These results were consistent with expected 2-D aerodynamics and showed no anomalies that could adversely affect the evaluation of anti-icing fluids and ice-pellet contamination on the wing. Tests conducted with roughness and leading-edge flow disturbances helped to explain the aerodynamic impact of the anti-icing fluids and contamination. The stalling characteristics of the wing section with fluid and contamination appear to be driven at least partially by the effects of a secondary wave of fluid that forms near the leading edge as the wing is rotated in the simulated takeoff profile. These results have provided a much more complete understanding of the adverse aerodynamic effects of anti-icing fluids and ice-pellet contamination on this wing section. This is important since these results are used, in part, to develop the ice-pellet allowance times that are applicable to many different airplanes.

Broeren, Andy P.↗

NASA NTRS2013

Aerodynamic Characterization of a Thin, High-Performance Airfoil for Use in Ground Fluids Testing

The FAA has worked with Transport Canada and others to develop allowance times for aircraft operating in ice-pellet precipitation. Wind-tunnel testing has been carried out to better understand the flowoff characteristics and resulting aerodynamic effects of anti-icing fluids contaminated with ice pellets using a thin, high-performance wing section at the National Research Council of Canada Propulsion and Icing Wind Tunnel. The objective of this paper is to characterize the aerodynamic behavior of this wing section in order to better understand the adverse aerodynamic effects of anti-icing fluids and ice-pellet contamination. Aerodynamic performance data, boundary-layer surveys and flow visualization were conducted at a Reynolds number of approximately 6.0 x 10(exp 6) and a Mach number of 0.12. The clean, baseline model exhibited leading-edge stall characteristics including a leading-edge laminar separation bubble and minimal or no separation on the trailing edge of the main element or flap. These results were consistent with expected 2-D aerodynamics and showed no anomalies that could adversely affect the evaluation of anti-icing fluids and ice-pellet contamination on the wing. Tests conducted with roughness and leading-edge flow disturbances helped to explain the aerodynamic impact of the anti-icing fluids and contamination. The stalling characteristics of the wing section with fluid and contamination appear to be driven at least partially by the effects of a secondary wave of fluid that forms near the leading edge as the wing is rotated in the simulated takeoff profile. These results have provided a much more complete understanding of the adverse aerodynamic effects of anti-icing fluids and ice-pellet contamination on this wing section. This is important since these results are used, in part, to develop the ice-pellet allowance times that are applicable to many different airplanes.

Broeren, Andy P.↗

NASA NTRS2011

Fluid Line Evacuation and Freezing Experiments for Digital Radiator Concept

The digital radiator technology is one of three variable heat rejection technologies being investigated for future human-rated NASA missions. The digital radiator concept is based on a mechanically pumped fluid loop with parallel tubes carrying coolant to reject heat from the radiator surface. A series of valves actuate to start and stop fluid flow to di erent combinations of tubes, in order to vary the heat rejection capability of the radiator by a factor of 10 or more. When the flow in a particular leg is stopped, the fluid temperature drops and the fluid can freeze, causing damage or preventing flow from restarting. For this reason, the liquid in a stopped leg must be partially or fully evacuated upon shutdown. One of the challenges facing fluid evacuation from closed tubes arises from the vapor generated during pumping to low pressure, which can cause pump cavitation and incomplete evacuation. Here we present a series of laboratory experiments demonstrating fluid evacuation techniques to overcome these challenges by applying heat and pumping to partial vacuum. Also presented are results from qualitative testing of the freezing characteristics of several different candidate fluids, which demonstrate significant di erences in freezing properties, and give insight to the evacuation process.

human space flight↗

NASA NTRS2015

Generalized Fluid System Simulation Program (GFSSP) - Version 6

The Generalized Fluid System Simulation Program (GFSSP) is a finite-volume based general-purpose computer program for analyzing steady state and time-dependent flow rates, pressures, temperatures, and concentrations in a complex flow network. The program is capable of modeling real fluids with phase changes, compressibility, mixture thermodynamics, conjugate heat transfer between solid and fluid, fluid transients, pumps, compressors, flow control valves and external body forces such as gravity and centrifugal. The thermo-fluid system to be analyzed is discretized into nodes, branches, and conductors. The scalar properties such as pressure, temperature, and concentrations are calculated at nodes. Mass flow rates and heat transfer rates are computed in branches and conductors. The graphical user interface allows users to build their models using the 'point, drag, and click' method; the users can also run their models and post-process the results in the same environment. The integrated fluid library supplies thermodynamic and thermo-physical properties of 36 fluids, and 24 different resistance/source options are provided for modeling momentum sources or sinks in the branches. Users can introduce new physics, non-linear and time-dependent boundary conditions through user-subroutine.

Majumdar, Alok↗

NASA NTRS2016

Proteomic Assessment of Fluid Shifts and Association with Visual Impairment and Intracranial Pressure in Twin Astronauts

BACKGROUND: Astronauts participating in long duration space missions are at an increased risk of physiological disruptions. The development of visual impairment and intracranial pressure (VIIP) syndrome is one of the leading health concerns for crew members on long-duration space missions; microgravity-induced fluid shifts and chronic elevated cabin CO2 may be contributing factors. By studying physiological and molecular changes in one identical twin during his 1-year ISS mission and his ground-based co-twin, this work extends a current NASA-funded investigation to assess space flight induced "Fluid Shifts" in association with the development of VIIP. This twin study uniquely integrates physiological and -omic signatures to further our understanding of the molecular mechanisms underlying space flight-induced VIIP. We are: (i) conducting longitudinal proteomic assessments of plasma to identify fluid regulation-related molecular pathways altered by long-term space flight; and (ii) integrating physiological and proteomic data with genomic data to understand the genomic mechanism by which these proteomic signatures are regulated. PURPOSE: We are exploring proteomic signatures and genomic mechanisms underlying space flight-induced VIIP symptoms with the future goal of developing early biomarkers to detect and monitor the progression of VIIP. This study is first to employ a male monozygous twin pair to systematically determine the impact of fluid distribution in microgravity, integrating a comprehensive set of structural and functional measures with proteomic, metabolomic and genomic data. This project has a broader impact on Earth-based clinical areas, such as traumatic brain injury-induced elevations of intracranial pressure, hydrocephalus, and glaucoma. HYPOTHESIS: We predict that the space-flown twin will experience a space flight-induced alteration in proteins and peptides related to fluid balance, fluid control and brain injury as compared to his pre-flight protein/peptide signatures. Conversely, the trajectory of these protein signatures will remain relatively constant in his ground based co-twin. METHODS: We are using proteomic and standard immunoelectrophoresis techniques to delineate the change in protein signatures throughout the course of a long duration space flight in relation to the development of VIIP. We are also applying a novel cell-based metaboloic organ system assay ("Organs on a Plate") to address how these circulating biomarkers affect physiological processes at the cellular and organ level which could result in VIIP symptoms. These molecular data will be correlated with physiological measures (eg. extra and intracellular fluid volume, vascular filling/flow patterns, MRI, and Optic Coherence Tomography. DISCUSSION: Pre- and in-flight data collection is in progress for the space-flown twin, and similar data have been obtained from the ground-based twin. Biosamples will be batch processed when received from ISS after the conclusion of the 1-year mission. Omic and Physiological measures from the twin astronauts will be compared to similar data being collected on twin subjects who participated in simulated microgravity study. bed rest study.

Rana, Brinda K.↗