Search NASA⌕ Search

SEARCH · Search NASA

Results for “internal standards”

Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 217 records · Page 12

Deformation and Flexibility Equations for Idealized ARIS Umbilicals, Under Planar End-Loading Conditions

The International Space Station (ISS) relies on the Active Rack Isolation System (ARIS) as the central component of an integrated, station-wide strategy to isolate microgravity space-science experiments. ARIS uses electromechanical actuators to isolate an International Standard Payload Rack (ISPR) from disturbances due to the motion of the ISS. Disturbances to microgravity experiments on ARIS-isolated racks are primarily transmitted via the ARTS power and vacuum umbilicals. Recent experimental tests indicate that these umbilicals resonate at frequencies outside the ARIS controller's bandwidth, at levels of potential concern for certain microgravity experiments. Reduction in the umbilical resonant frequencies could help to address this issue. This report develops equations for the in-plane deflections and flexibilities of an idealized umbilical (thin, flexible, cantilever beam) under end-point, in-plane loading (inclined-force and moment). The effect of gravity is neglected due to the on-orbit application. The analysis assumes an initially straight, cantilevered umbilical with uniform cross-section, which undergoes large deflections with no plastic deformation, such that the umbilical terminus remains in a single quadrant and the umbilical slope changes monotonically. The analysis is applicable to the ARIS power and vacuum umbilicals, under the indicated assumptions.

Hampton, R. David↗

ASK Talks with Dennis Grounds

Dennis Grounds recently finished a one-year assignment at NASA Headquarters in the Office of Bioastronautics as the Acting Flight Program Manager He has returned to Johnson Space Center (JSC), where he is Director of the International Space Station Bioastronautics Research Program Office with the NASA Life Sciences Projects Division. Under his management, the Human Research Facility (HRF) was developed to support a broad range of scientific investigations pertaining to human adaptation to the spaceflight environment and issues of human space exploration. The HRF rack was developed to international standards in order to be compatible with payloads developed anywhere in the world, thereby streamlining the process of getting payloads on the Space Station. Grounds has worked with NASA for more than 15 years. Prior to joining ISS, he worked with General Electric as a manager of payloads and analysis in support of the NASA Life Science Projects Division at JSC. ASK spoke with Grounds in Washington, D.C., during his Headquarters assignment.

Source record↗

The Interplanetary Internet: a communications infrastructure for Mars exploration

A strategy is being developed whereby the current set of internationally standardized space data communications protocols can be incrementally evolved so that a first version of an operational "Interplanetary Internet" is feasible by the end of the decade. This paper describes its architectural concepts, discusses the current set of standard space data communications capabilities that exist to support Mars exploration and reviews proposed new developments. We also speculate that these current capabilities can grow to support future scenarios where human intelligence is widely distributed across the Solar System and day-to-day communications dialog between planets is routine. c2003 American Institute of Aeronautics and Astronautics. Published by Elsevier Science Ltd. All rights reserved.

Internet/standards↗

Optimization of an oligonucleotide microchip for microbial identification studies: a non-equilibrium dissociation approach

The utility of a high-density oligonucleotide microarray (microchip) for identifying strains of five closely related bacilli (Bacillus anthracis, Bacillus cereus, Bacillus mycoides, Bacillus medusa and Bacillus subtilis) was demonstrated using an approach that compares the non-equilibrium dissociation rates ('melting curves') of all probe-target duplexes simultaneously. For this study, a hierarchical set of 30 oligonucleotide probes targeting the 16S ribosomal RNA of these bacilli at multiple levels of specificity (approximate taxonomic ranks of domain, kingdom, order, genus and species) was designed and immobilized in a high-density matrix of gel pads on a glass slide. Reproducible melting curves for probes with different levels of specificity were obtained using an optimized salt concentration. Clear discrimination between perfect match (PM) and mismatch (MM) duplexes was achieved. By normalizing the signals to an internal standard (a universal probe), a more than twofold discrimination (> 2.4x) was achieved between PM and 1-MM duplexes at the dissociation temperature at which 50% of the probe-target duplexes remained intact. This provided excellent differentiation among representatives of different Bacillus species, both individually and in mixtures of two or three. The overall pattern of hybridization derived from this hierarchical probe set also provided a clear 'chip fingerprint' for each of these closely related Bacillus species.

Non-NASA Center↗

8th Spacecraft Charging Technology Conference

The 8th Spacecraft Charging Technology Conference was held in Huntsville, Alabama, October 20-24, 2003. Hosted by NASA s Space Environments and Effects (SEE) Program and co-sponsored by the Air Force Research Laboratory (AFRL) and the European Space Agency (ESA), the 2003 conference saw attendance from eleven countries with over 65 oral papers and 18 poster papers. Presentation topics highlighted the latest in spacecraft charging mitigation techniques and on-orbit investigations, including: Plasma Propulsion and Tethers; Ground Testing Techniques; Interactions of Spacecraft and Systems With the Natural and Induced Plasma Environment; Materials Characterizations; Models and Computer Simulations; Environment Specifications; Current Collection and Plasma Probes in Space Plasmas; On-Orbit Investigations. A round-table discussion of international standards regarding electrostatic discharge (ESD) testing was also held with the promise of continued discussions in the off years and an official continuation at the next conference.

Minor, J. L.↗

Analysis of myosin heavy chain mRNA expression by RT-PCR

An assay was developed for rapid and sensitive analysis of myosin heavy chain (MHC) mRNA expression in rodent skeletal muscle. Only 2 microg of total RNA were necessary for the simultaneous analysis of relative mRNA expression of six different MHC genes. We designed synthetic DNA fragments as internal standards, which contained the relevant primer sequences for the adult MHC mRNAs type I, IIa, IIx, IIb as well as the embryonic and neonatal MHC mRNAs. A known amount of the synthetic fragment was added to each polymerase chain reaction (PCR) and yielded a product of different size than the amplified MHC mRNA fragment. The ratio of amplified MHC fragment to synthetic fragment allowed us to calculate percentages of the gene expression of the different MHC genes in a given muscle sample. Comparison with the traditional Northern blot analysis demonstrated that our reverse transcriptase-PCR-based assay was reliable, fast, and quantitative over a wide range of relative MHC mRNA expression in a spectrum of adult and neonatal rat skeletal muscles. Furthermore, the high sensitivity of the assay made it very useful when only small quantities of tissue were available. Statistical analysis of the signals for each MHC isoform across the analyzed samples showed a highly significant correlation between the PCR and the Northern signals as Pearson correlation coefficients ranged between 0.77 and 0.96 (P < 0.005). This assay has potential use in analyzing small muscle samples such as biopsies and samples from pre- and/or neonatal stages of development.

Non-NASA Center↗

Deformation and Flexibility Equations for ARIS Umbilicals Idealized as Planar Elastica

The International Space Station relies on the active rack isolation system (ARIS) as the central component of an integrated, stationwide strategy to isolate microgravity space-science experiments. ARIS uses electromechanical actuators to isolate an international standard payload rack from disturbances due to the motion of the Space Station. Disturbances to microgravity experiments on ARIS isolated racks are transmitted primarily via the ARIS power and vacuum umbilicals. Experimental tests indicate that these umbilicals resonate at frequencies outside the ARIS controller s bandwidth at levels of potential concern for certain microgravity experiments. Reduction in the umbilical resonant frequencies could help to address this issue. This work documents the development and verification of equations for the in-plane deflections and flexibilities of an idealized umbilical (thin, flexible, inextensible, cantilever beam) under end-point, in-plane loading (inclined-force and moment). The effect of gravity is neglected due to the on-orbit application. The analysis assumes an initially curved (not necessarily circular), cantilevered umbilical with uniform cross-section, which undergoes large deflections with no plastic deformation, such that the umbilical slope changes monotonically. The treatment is applicable to the ARIS power and vacuum umbilicals under the indicated assumptions.

Hampton, R. David↗

Potential Market for Satellite Technology in Meeting Telecommunication Needs of Developing Nations

A recent study examined the potential for satellite technology to meet the telecommunication needs of developing nations. The growth of these nations depends on their attracting and holding the industrial investments of developed nations. This will not be likely with the antiquated telecommunications infrastructure typical of developing nations. On the contrary, it will require an infrastructure that is compatible with international standards. Most of the developing nations perceive this necessity and are pursuing the necessary upgrades. The rate of replacement, types of technology, services affected, and the terrestrial/satellite mix differ by each nation's priorities and gross national product (GNP).

Source record↗

Fluids and Combustion Facility: Combustion Integrated Rack Modal Model Correlation

The Fluids and Combustion Facility (FCF) is a modular, multi-user, two-rack facility dedicated to combustion and fluids science in the US Laboratory Destiny on the International Space Station. FCF is a permanent facility that is capable of accommodating up to ten combustion and fluid science investigations per year. FCF research in combustion and fluid science supports NASA's Exploration of Space Initiative for on-orbit fire suppression, fire safety, and space system fluids management. The Combustion Integrated Rack (CIR) is one of two racks in the FCF. The CIR major structural elements include the International Standard Payload Rack (ISPR), Experiment Assembly (optics bench and combustion chamber), Air Thermal Control Unit (ATCU), Rack Door, and Lower Structure Assembly (Input/Output Processor and Electrical Power Control Unit). The load path through the rack structure is outlined. The CIR modal survey was conducted to validate the load path predicted by the CIR finite element model (FEM). The modal survey is done by experimentally measuring the CIR frequencies and mode shapes. The CIR model was test correlated by updating the model to represent the test mode shapes. The correlated CIR model delivery is required by NASA JSC at Launch-10.5 months. The test correlated CIR flight FEM is analytically integrated into the Shuttle for a coupled loads analysis of the launch configuration. The analysis frequency range of interest is 0-50 Hz. A coupled loads analysis is the analytical integration of the Shuttle with its cargo element, the Mini Payload Logistics Module (MPLM), in the Shuttle cargo bay. For each Shuttle launch configuration, a verification coupled loads analysis is performed to determine the loads in the cargo bay as part of the structural certification process.

McNelis, Mark E.↗

Library Services Funding Assessment

The Glenn Technical Library is a science and engineering library that primarily supports research activities at the Glenn Research Center, and provides selected services to researchers at all of the NASA research centers. Resources available in the library include books, journals, CD-ROMs, and access to various online sources, as well as live reference and inter-library loan services. The collection contains over 77,000 books, 800,000 research reports, and print or online access to over 1,400 journals. Currently the library operates within the Logistics and Technical Information Division, and is funded as an open-access resource within the GRC. Some of the research units at the GRC have recently requested that the library convert to a "pay-for-services" model, in which individual research units could fund only those journal subscriptions for which they have a specific need. Under this model, the library would always maintain a certain minimum level of pooled-expense services, including the ready reference and book collections, and inter-library loan services. Theoretically the "pay-for-services" model would encourage efficient financial allocation, and minimize the extent to which paid journal subscriptions go unused. However, this model also could potentially negate the benefits of group purchases for journal subscriptions and access. All of the major journal publishers offer package subscriptions that compare favorably in cost with the sum of individual subscription costs for a similar selection of titles. Furthermore, some of these subscription packages are "consortium" purchases that are funded collectively by the libraries at multiple NASA research centers; such consortia1 memberships would be difficult for the library to pay, if enough GRC research units were to withdraw their pooled contributions. cost of collectively-funded journal access with the cost of individual subscriptions. My primary task this summer is to create the cost dataset framework, and collect as much of the relevant data as possible. Hopefully this dataset will permit the research units at the GRC, and library administration as well, to make informed decisions about future library funding. Prior to the creation of the actual dataset, I established a comprehensive list of the library s print and online journal subscriptions. This list will be useful outside the context of the cost analysis project, as an addition to the library website. The cost analysis dataset s primary fields are: journal name, vendor, publisher, ISSN (International Standard Serial Number, to uniquely identify the titles), stand-alone price, and indication as to the presence of the journal in current GRC Technical Library consortium membership subscriptions. The dataset will hopefully facilitate comparisons between the stand-alone journal prices and the cost of shared journal subscriptions for groups of titles.

Lorig, Jonathan A.↗

The Fluids and Combustion Facility

Microgravity is an environment with very weak gravitational effects. The Fluids and Combustion Facility (FCF) on the International Space Station (ISS) will support the study of fluid physics and combustion science in a long-duration microgravity environment. The Fluid Combustion Facility's design will permit both independent and remote control operations from the Telescience Support Center. The crew of the International Space Station will continue to insert and remove the experiment module, store and reload removable data storage and media data tapes, and reconfigure diagnostics on either side of the optics benches. Upon completion of the Fluids Combustion Facility, about ten experiments will be conducted within a ten-year period. Several different areas of fluid physics will be studied in the Fluids Combustion Facility. These areas include complex fluids, interfacial phenomena, dynamics and instabilities, and multiphase flows and phase change. Recently, emphasis has been placed in areas that relate directly to NASA missions including life support, power, propulsion, and thermal control systems. By 2006 or 2007, a Fluids Integrated Rack (FIR) and a Combustion Integrated Rack (CIR) will be installed inside the International Space Station. The Fluids Integrated Rack will contain all the hardware and software necessary to perform experiments in fluid physics. A wide range of experiments that meet the requirements of the international space station, including research from other specialties, will be considered. Experiments will be contained in subsystems such as the international standard payload rack, the active rack isolation system, the optics bench, environmental subsystem, electrical power control unit, the gas interface subsystem, and the command and data management subsystem. In conclusion, the Fluids and Combustion Facility will allow researchers to study fluid physics and combustion science in a long-duration microgravity environment. Additional information is included in the original extended abstract.

Kundu, Sampa↗

A "Kane's Dynamics" Model for the Active Rack Isolation System: Addition of Umbilicals to the Nonlinear Model - Part 3

In the late 1980s, microgravity researchers began to voice their concern that umbilical-transmitted energy could significantly degrade the acceleration environment of microgravity space science experiments onboard manned spacecraft. Since umbilicals are necessary for many experiments, control designers began to seek ways to compensate for these "indirect" disturbances. Hampton, et al., used the Kane s method to develop a model of the active rack isolation system (ARIS) that includes (1) actuator control forces, (2) direct disturbance forces, and (3) indirect, actuator-transmitted disturbances. Their model does not, however, include the indirect, umbilical-transmitted disturbances. Since the umbilical stiffnesses are not negligible, these indirect disturbances must be included in the model. Until the umbilicals have been appropriately included, the model will be incomplete. This Technical Memorandum presents a nonlinear model of ARIS with umbilicals included. Model verification was achieved by utilizing two commercial-off-the-shelf software tools. Various forces and moments were applied to the model to yield simulated responses of the system. Plots of the simulation results show how various critical points on an ARIS-outfitted international standard payload rack behave under the application of direct disturbances, indirect disturbances, and control forces. Simulations also show system response to a variety of initial conditions.

Rupert, J. K.↗

LC/MS Method for the Determination of Stable Isotope Labeled Promethazine in Human Plasma

Promethazine (PMZ) is taken by astronauts orally (PO), intramuscularly (IM) or rectally (PR) for space motion sickness. LC/MS method was developed with off-line solid phase extraction to measure plasma concentrations of PMZ given as stable isotope-labeled (SIL) formulations by the three different routes of administration simultaneously. Samples (0.5ml) were loaded on to Waters Oasis HLB co-polymer cartridges and eluted with 1.0 mL methanol. HPLC separation of the eluted sample was performed using an Agilent Zorbax SB-CN column (50 x 2.1 mm) at a flow rate of 0.2 mL/min for 6 min. Acetonitrile/ ammonium acetate (30 mM) in water (3:2, v/v), pH 5.6 plus or minus 0.1, was used as the mobile phase for separation. Concentrations of PMZ, PMZ-d4 and PMZ-d7 and chlorpromazine (internal standard) were determined using a Micromass ZMD single quadrupole mass spectrometer with Electrospray Ionization (ESI). ESI mass spectra were acquired in positive ion mode with selected ion monitoring of [M+ H]dot plus. The method is rapid, reproducible and the assay specific parameters are listed in a table. A novel, sensitive and specific method for the measurement of PMZ and SIL PMZ in human plasma is reported.

Zuwei, Wang↗

International NDT Technical Collaboration Using the Internet

This paper reviews the capability of the Internet as a world-wide NDT communication tool for technical collaboration, dissemination of information and international standards. This paper was written by authors that never personally met (except for two) and despite thousands of miles distance between them the authors managed to work as a team to complete this paper in a very time efficient manner. This success is attributed to the power of the Internet and it represents a simple example of the.

Internet communication information exchange web si↗

Laser Opto-Electronic Correlator for Robotic Vision Automated Pattern Recognition

A compact laser opto-electronic correlator for pattern recognition has been designed, fabricated, and tested. Specifically it is a translation sensitivity adjustable compact optical correlator (TSACOC) utilizing convergent laser beams for the holographic filter. Its properties and performance, including the location of the correlation peak and the effects of lateral and longitudinal displacements for both filters and input images, are systematically analyzed based on the nonparaxial approximation for the reference beam. The theoretical analyses have been verified in experiments. In applying the TSACOC to important practical problems including fingerprint identification, we have found that the tolerance of the system to the input lateral displacement can be conveniently increased by changing a geometric factor of the system. The system can be compactly packaged using the miniature laser diode sources and can be used in space by the National Aeronautics and Space Administration (NASA) and ground commercial applications which include robotic vision, and industrial inspection of automated quality control operations. The personnel of Standard International will work closely with the Jet Propulsion Laboratory (JPL) to transfer the technology to the commercial market. Prototype systems will be fabricated to test the market and perfect the product. Large production will follow after successful results are achieved.

Laser Opto-Electronic↗

DSMS investment in support of satellite constellations and formation flying

Over the years, NASA has supported unmanned space missions, beyond earth orbit, through a Deep Space Mission System (DSMS) that is developed and operated by the Jet Propulsion Laboratory (JPL) and subcontractors. The DSMS capabilities have been incrementally upgraded since its establishment in the late '50s and are delivered primarily through three Deep Space Communications Complexes (DSCC 's) near Goldstone, California, Madrid, Spain, and Canberra, Australia and from facilities at JPL. Traditionally, mission support (tracking, command, telemetry, etc) is assigned on an individual-mission basis, between each mission and a ground-based asset, independent of other missions. As NASA, and its international partners, move toward flying fullconstellations and precision formations, the DSMS is developing plans and technologies to provide the requisite support. The key activities under way are: (1) integrated communications architecture for Mars exploration, including relays on science orbiters and dedicated relay satellites to provide continuous coverage for orbiters, landers and rovers. JPL is developing an architecture, as well as protocols and equipment, required for the cost-effective operations of such an infrastructure. (2) Internet-type protocols that will allow for efficient operations across the deep-space distances, accounting for and accommodating the long round-trip-light-time. JPL is working with the CCSDS to convert these protocols to an international standard and will deploy such protocol, the CCSDS File Delivery Protocol (CFDP), on the Mars Reconnaissance Orbiter (MRO) and on the Deep Impact (01) missions. (3) Techniques to perform cross-navigation between spacecrafi that fly in a loose formation. Typical cases are cross-navigation between missions that approach Mars and missionsthat are at Mars, or the determination of a baseline for missions that fly in an earth-lead- lag configuration. (4) Techniques and devices that allow the precise metrology and controllability of tightformations for precision constellation missions. In this paper we discuss the four classes of constellatiodformation support with emphasis of DSMS current status (technology and implementation) and plans in the first three areas.

space operations↗

Coloration Determination of Spectral Darkening Occurring on a Broadband Earth Observing Radiometer: Application to Clouds and the Earth's Radiant Energy System (CERES)

It is estimated that in order to best detect real changes in the Earth s climate system, space based instrumentation measuring the Earth Radiation Budget (ERB) must remain calibrated with a stability of 0.3% per decade. Such stability is beyond the specified accuracy of existing ERB programs such as the Clouds and the Earth s Radiant Energy System (CERES, using three broadband radiometric scanning channels: the shortwave 0.3 - 5microns, total 0.3. > 100microns, and window 8 - 12microns). It has been shown that when in low earth orbit, optical response to blue/UV radiance can be reduced significantly due to UV hardened contaminants deposited on the surface of the optics. Since typical onboard calibration lamps do not emit sufficient energy in the blue/UV region, this darkening is not directly measurable using standard internal calibration techniques. This paper describes a study using a model of contaminant deposition and darkening, in conjunction with in-flight vicarious calibration techniques, to derive the spectral shape of darkening to which a broadband instrument is subjected. Ultimately the model uses the reflectivity of Deep Convective Clouds as a stability metric. The results of the model when applied to the CERES instruments on board the EOS Terra satellite are shown. Given comprehensive validation of the model, these results will allow the CERES spectral responses to be updated accordingly prior to any forthcoming data release in an attempt to reach the optimum stability target that the climate community requires.

Matthews, Grant↗

Asynchronous Message Service for Deep Space Mission Operations

While the CCSDS (Consultative Committee for Space Data Systems) File Delivery Protocol (CFDP) provides internationally standardized file transfer functionality that can offer significant benefits for deep space mission operations, not all spacecraft communication requirements are necessarily best met by file transfer. In particular, continuous event-driven asynchronous message exchange may also be useful for communications with, among, and aboard spacecraft. CCSDS has therefore undertaken the development of a new Asynchronous Message Service (AMS) standard, designed to provide common functionality over a wide variety of underlying transport services, ranging from shared memory message queues to CCSDS telemetry systems. The present paper discusses the design concepts of AMS, their applicability to deep space mission operations problems, and the results of preliminary performance testing obtained from exercise of a prototype implementation.

asynchronous message exchanges↗