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Nutritional Status in Humans during Long-Duration Bed Rest

Bed rest is a valuable ground-based model for many of the physiological changes associated with space flight. A series of studies was undertaken to evaluate nutritional changes during and after 60 or 90 days of -6 head-down-tilt bed rest. A total of 11 subjects (8 M, 3 F; age 26-55 y) participated in the studies. Blood and urine were collected twice before bed rest and once per month during bed rest. Samples were analyzed in batch at the end of each study. Data were analyzed using repeated-measures ANOVA. Markers of bone resorption (such as n-telopeptide excretion, p less than 0.05) increased during bed rest, and 25-OH vitamin D status tended to decline (p=0.06). During bed rest oxidative damage markers, such as superoxide dismutase increased (p less than 0.01) and 8-(OH)-2'-deoxyguanosine tended to increase (p=0.07); whereas total antioxidant capacity decreased (p less than 0.02). Iron status indices showed patterns of increased iron stores, with decreased transferrin receptors (p less than 0.001). Biochemical markers revealed a tendency toward a loss of muscle mass, by lower excretion of creatinine and 3-methyl-histidine during bed rest. All of these changes are very similar to those observed during space flight, and further document the utility of bed rest as a model of space flight.

Smith, Scott M.

Combined VSWIR/TIR Products Overview: Issues and Examples

The presentation provides a summary of VSWIR data collected at 19-day intervals for most areas. TIR data was collected both day and night on a 5-day cycle (more frequently at higher latitudes), the TIR swath is four times as wide as VSWIR, and the 5-day orbit repeat is approximate. Topics include nested swath geometry for reference point design and coverage simulations for sample FLUXNET tower sites. Other points examined include variation in latitude for revisit frequency, overpass times, and TIR overlap geometry and timing between VSWIR data collections.

Knox, Robert G.

Pulmonary Inflammatory Responses To Acute Meteorite Dust Exposures - Implications For Human Space Exploration

The previous manned missions to the Moon represent milestones of human ingenuity, perseverance, and intellectual curiosity. However, one of the major ongoing concerns is the array of hazards associated with lunar surface dust. Not only did the dust cause mechanical and structural integrity issues with the suits, the dust 'storm' generated upon reentrance into the crew cabin caused "lunar hay fever" and "almost blindness" (Figure 1). It was further reported that the allergic response to the dust worsened with each exposure. The lack of gravity exacerbated the exposure, requiring the astronauts to wear their helmet within the module in order to avoid breathing the irritating particles. Due to the prevalence of these high exposures, the Human Research Roadmap developed by NASA identifies the Risk of Adverse Health and Performance Effects of Celestial Dust Exposure as an area of concern. Extended human exploration will further increase the probability of inadvertent and repeated exposures to celestial dusts. Going forward, hazard assessments of celestial dusts will be determined through sample return efforts prior to astronaut deployment. Studies on the lunar highland regolith indicate that the dust is not only respirable but also reactive, and previous studies concluded that it is moderately toxic; generating a greater response than titanium oxide but a lower response than quartz. The presence of reactive oxygen species (ROS) on the surface of the dust has been implicated. However, there is actually little data related to physicochemical characteristics of particulates and pulmonary toxicity, especially as it relates to celestial dust exposure. As a direct response to this deficit, the present study evaluates the role of a particulate's innate geochemical features (e.g., bulk chemistry, internal composition, morphology, size, and reactivity) in generating adverse toxicological responses in vitro and in vivo. This highly interdisciplinary study evaluates the relative toxicity of six meteorite samples representing either basalt or regolith breccia on the surfaces of the Moon, Mars, and Asteroid 4Vesta; three potential candidates for future human exploration or colonization. Terrestrial mid-ocean ridge basalt (MORB) is also used for comparison as a control sample.

Harrington, A. D.

Acute Meteorite Dust Exposure and Pulmonary Inflammation - Implications for Human Space Exploration

The previous manned missions to the Moon represent milestones of human ingenuity, perseverance, and intellectual curiosity. However, one of the major ongoing concerns is the array of hazards associated with lunar surface dust. Not only did the dust cause mechanical and structural integrity issues with the suits, the dust 'storm' generated upon reentrance into the crew cabin caused "lunar hay fever" and "almost blindness [1-3]" (Figure 1). It was further reported that the allergic response to the dust worsened with each exposure [4]. The lack of gravity exacerbated the exposure, requiring the astronauts to wear their helmet within the module in order to avoid breathing the irritating particles [1]. Due to the prevalence of these high exposures, the Human Research Roadmap developed by NASA identifies the Risk of Adverse Health and Performance Effects of Celestial Dust Exposure as an area of concern [5]. Extended human exploration will further increase the probability of inadvertent and repeated exposures to celestial dusts. Going forward, hazard assessments of celestial dusts will be determined through sample return efforts prior to astronaut deployment. Studies on the lunar highland regolith indicate that the dust is not only respirable but also reactive [2, 6-9], and previous studies concluded that it is moderately toxic; generating a greater response than titanium oxide but a lower response than quartz [6]. The presence of reactive oxygen species (ROS) on the surface of the dust has been implicated. However, there is actually little data related to physicochemical characteristics of particulates and pulmonary toxicity, especially as it relates to celestial dust exposure. As a direct response to this deficit, the present study evaluates the role of a particulate's innate geochemical features (e.g., bulk chemistry, internal composition, morphology, size, and reactivity) in generating adverse toxicological responses in vitro and in vivo. This highly interdisciplinary study evaluates the relative toxicity of six meteorite samples representing either basalt or regolith breccia on the surfaces of the Moon, Mars, and Asteroid 4Vesta (Table 1); three potential candidates for future human exploration or colonization. Terrestrial mid-ocean ridge basalt (MORB) is also used for comparison as a control sample.

Harrington, A. D.

The Need for Medical Geology in Space Exploration: Implications for the Journey to Mars and Beyond

The previous manned missions to the Moon represent milestones in human ingenuity, perseverance, and intellectual curiosity. They also highlight a major hazard for future human exploration of the Moon and beyond: surface dust. Not only did the dust cause mechanical and structural integrity issues with the suits, the dust "storm" generated upon reentrance into the crew cabin caused "lunar hay fever" and "almost blindness". It was further reported that the allergic response to the dust worsened with each exposure. The lower gravity environment exacerbated the exposure, requiring the astronauts to wear their helmet within the module in order to avoid breathing the irritating particles. Due to the prevalence of these high exposures, the Human Research Roadmap developed by NASA identifies the Risk of Adverse Health and Performance Effects of Celestial Dust Exposure as an area of concern. Extended human exploration will further increase the probability of inadvertent and repeated exposures to celestial dusts. Going forward, hazard assessments of celestial dusts will be determined through sample return efforts prior to astronaut deployment. However, even then the returned samples could also put the Curators, technicians, and scientists at risk during processing and examination.

Harrington, A. D.

The Integrated Impact of Diet on Human Immune Response, the Gut Microbiota, and Nutritional Status During Adaptation to Spaceflight

Long-duration spaceflight impacts human physiology, including well documented immune system dysregulation. Diet, the microbiome, and immune system function are interlinked, but diet is the only one of these factors that we have the ability to easily, and significantly, alter on Earth or during flight. As we better understand dietary impacts on physiology, we may then improve the spaceflight diet to improve crew health and potentially reduce spaceflight-associated physiological decrements. Increasing the consumption of fruits and vegetables and bioactive compounds (e.g., omega-3 fatty acids, lycopene, flavonoids) and therefore enhancing overall nutritional intake from the nominal shelf-stable, fully-processed, space food system is expected to serve as a countermeasure to detrimental impacts to human physiology, including dysregulation in immunological profiles, the taxonomic profile of the gut microbiota, and nutritional status during spaceflight. In this study, first we sought to determine the effect of the nominal shelf-stable spaceflight diet compared to an "enhanced" shelf-stable spaceflight diet on human biochemistry, immunology, and the microbiome in a ground-based, simulated space mission. The ground analog portion of this study was conducted in the NASA Human Exploration Research Analog (HERA) Campaign 4 missions, which consisted of four 45-day missions with closed chamber confinement and realistic mission simulation to study effects on crew health and performance. As reported previously, analyses indicate beneficial associations between diet and markers of nutritional status, stress, the microbiome, and cognitive performance. Intake and beneficial associations varied by subject. This data will be used as a ground-based control for spaceflight, where the spaceflight environment (e.g., radiation, microgravity) will have additional impacts and the potential to evaluate effects of the diet will be greater. The second phase of this study is to occur on the International Space Station, where it is currently being implemented. The test plan is similar to that used in the HERA missions. The enhanced diet is intended to provide 25% of the crews’ diet with foods rich in omega-3 fatty acids, lycopene, and flavonoids, along with more fruits and vegetables in general (the other 75% of the diet will be obtained from standard and crew preference items available on the ISS). Biological samples (blood, urine, stool, and saliva) are being collected from participants at selected time points before, during, and after the mission. Data collection also includes dietary intake recording and body mass measurement. Currently, 6 of 9 planned astronauts have completed data collection. Analysis of immune markers, latent herpes virus reactivation, the taxonomic and metatranscriptomic profile of the gut microbiome, and nutritional status biomarkers and biochemical metabolites will occur in batch to minimize sample handling variations. Mixed models statistical analyses will be used, incorporating random effects to account for repeated measures within individuals to assess the impact of diet on physiological outcomes. We expect this study to provide evidence of beneficial impact of this enhanced diet on crew health and adaptation to spaceflight. These data will aid in evidence-based mass-risk trades for food system design and development of targeted dietary interventions for future exploration-class space missions.

Grace L. Douglas

Resolving the fine structure in the energy landscapes of repeat proteins

Ankyrin (ANK) repeat proteins are coded by tandem occurrences of patterns with around 33 amino acids. They often mediate protein–protein interactions in a diversity of biological systems. These proteins have an elongated non-globular shape and often display complex folding mechanisms. This work investigates the energy landscape of representative proteins of this class made up of 3, 4 and 6 ANK repeats using the energy-landscape visualisation method (ELViM). By combining biased and unbiased coarse-grained molecular dynamics AWSEM simulations that sample conformations along the folding trajectories with the ELViM structure-based phase space, one finds a three-dimensional representation of the globally funnelled energy surface. In this representation, it is possible to delineate distinct folding pathways. We show that ELViMs can project, in a natural way, the intricacies of the highly dimensional energy landscapes encoded by the highly symmetric ankyrin repeat proteins into useful low-dimensional representations. These projections can discriminate between multiplicities of specific parallel folding mechanisms that otherwise can be hidden in oversimplified depictions.

Murilo N. Sanches

Sample Acquisition and Analytical Chemistry Challenges to Verifying Compliance to Aviators Breathing Oxygen (ABO) Purity Specification

NASA has been developing and testing two different types of oxygen separation systems. One type of oxygen separation system uses pressure swing technology, the other type uses a solid electrolyte electrochemical oxygen separation cell. Both development systems have been subjected to long term testing, and performance testing under a variety of environmental and operational conditions. Testing these two systems revealed that measuring the product purity of oxygen, and determining if an oxygen separation device meets Aviator's Breathing Oxygen (ABO) specifications is a subtle and sometimes difficult analytical chemistry job. Verifying product purity of cryogenically produced oxygen presents a different set of analytical chemistry challenges. This presentation will describe some of the sample acquisition and analytical chemistry challenges presented by verifying oxygen produced by an oxygen separator - and verifying oxygen produced by cryogenic separation processes. The primary contaminant that causes gas samples to fail to meet ABO requirements is water. The maximum amount of water vapor allowed is 7 ppmv. The principal challenge of verifying oxygen produced by an oxygen separator is that it is produced relatively slowly, and at comparatively low temperatures. A short term failure that occurs for just a few minutes in the course of a 1 week run could cause an entire tank to be rejected. Continuous monitoring of oxygen purity and water vapor could identify problems as soon as they occur. Long term oxygen separator tests were instrumented with an oxygen analyzer and with an hygrometer: a GE Moisture Monitor Series 35. This hygrometer uses an aluminum oxide sensor. The user's manual does not report this, but long term exposure to pure oxygen causes the aluminum oxide sensor head to bias dry. Oxygen product that exceeded the 7 ppm specification was improperly accepted, because the sensor had biased. The bias is permanent - exposure to air does not cause the sensor to return to its original response - but the bias can be accounted for by recalibrating the sensor. After this issue was found, continuous measurements of water vapor in the oxygen product were made using an FTIR. The FTIR cell is relatively large, so response time is slow - but moisture measurements were repeatable and accurate. Verifying ABO compliance for oxygen produced by commercial cryogenic processes has a different set of sample acquisition and analytical chemistry challenges. Customers want analytical chemists to conserve as much as possible. Hygrometers are not exposed to hours of continuous flow of oxygen, so they don't bias, but small amounts of contamination in valves can cause a "fail". K bottles are periodically cleaned and recertified - after cleaning residual moisture can cause a "fail". Operators let bottle pressure drop to room pressure, introduce outside air into the bottle, and the subsequent fill will "fail". Outside storage of K‐bottles has allowed enough in‐leakage, so contents will "fail".

Graf, John

Investigating Soil Organic Matter Complexation using Spectral Induced Polarization

Spectral induced polarization (SIP) laboratory experiments were conducted to determine the sensitivity of this method to the formation of soil organic matter (SOM) complexes, with a long-term goal of field-scale monitoring. There are few SIP experiments that have explored this topic, yet understanding the dynamic behavior and interactions of SOM at the field scale could provide insight into soil fertility and health which influences crop yields, microorganisms that degrade organic pollutants, and carbon stabilization. We present the results of three experiments where the iron oxide, ferrihydrite (Fhy), was used to coat different media, and then the OM compound pentaglycine (PG) was pulse injected to form SOM complexes. SIP data was collected during these injections to capture any surface complexation changes. These experiments were performed in 1) a fluidic cell containing a micromodel, 2) a column containing Fhy coated ceramic beads and 3) a column containing Fhy coated Accusand®. Our results show a higher frequency response (defined here as > 1 Hz) in all three experiments, with the largest amplitude response after the first PG injection (Figure S.1). The repeatability of this response is encouraging and supporting data collected on the Accusand® experiment provides preliminary insight into the mechanisms controlling the SIP signatures. Sampling of fluid conductivity $σ_w$ and pH may indicate deprotonation of SOM occurring or rapid adsorption and release of protons from the Fhy sites. However additional experiments are needed to identify and confirm the primary and secondary reactions impacting the SIP response. We are looking towards other opportunities to continue this work, particularly to repeat experiments while collecting supporting datasets.

58 GEOSCIENCES

The Ad Hoc Mars Airplane science working group

The capability of a remotely piloted airplane as a Mars exploration vehicle in the aerial survey mode is assessed. Specific experiment areas covered include: visual imaging; gamma ray and infrared reflectance spectroscopy; gravity field; magnetic field and electromagnetic sounding; and atmospheric composition and dynamics. It is concluded that (1) the most important use of a plane in the aerial survey mode would be in topical studies and returned sample site characterization; (2) the airplane offers the unique capability to do high resolution, oblique imaging, and repeated profile measurements in the atmospheric boundary layer; and (3) it offers the best platform from which to do electromagnetic sounding.

Clarke, V. C., Jr.

An analysis of the frequency characteristics of corona discharge at low pressure

Corona discharge is becoming more of a concern now that the space program is looking at higher power and voltage requirements for future missions. Improved detection methods for studying corona discharge will increase the knowledge base of how this phenomenon occurs, what effects it has on power systems, and how it is affected by outside influences. A better understanding of corona discharge will enable designers to improve on the space applications of insulation and dielectrics, and develop proper detection techniques. A test system was designed and constructed to measure the frequency spectrum of corona discharge current in low pressure. Various test samples in different arrangements were energized by ac voltage until corona discharge occurred. This same test was repeated at different pressures. The results indicate that the frequency spectrum of the discharge current changes with a change in pressure. At low pressures, the discharge frequency spectrum is concentrated in a narrow band with a peak at 17 kHz. As the pressure is increased, the spectral width increases to include components across a much broader band. Other tests were performed to correlate the frequency spectrum data to the shape of the discharge pulse and the physical appearance of the glow discharge.

T Gregory Lewis

Transonic Semispan Aerodynamic Testing of the Hybrid Wing Body with Over Wing Nacelles in the National Transonic Facility

A wind tunnel investigation of a 0.04-scale model of the Lockheed Martin Hybrid Wing Body (HWB) with Over Wing Nacelles (OWN) air mobility transport configuration was conducted in the National Transonic Facility at the NASA Langley Research Center under a collaborative partnership between NASA, the Air Force Research Laboratory, and Lockheed Martin Aeronautics Company. The wind tunnel test sought to validate the transonic aerodynamic performance of the HWB and to validate the efficiency benefits of the OWN installation as compared to the traditional under-wing installation. The semispan HWB model was tested in a clean wing configuration and also tested with two different nacelles representative of a modern turbofan engine and a future advanced high bypass ratio engine. The nacelles were installed in three different locations with two over-wing positions and one under-wing position. Five-component force and moment data, surface static pressure data, and aeroelastic deformation data were acquired. For the cruise configuration, the model was tested in an angle-of-attack range between -2 and 10 degrees at free-stream Mach numbers from 0.3 to 0.9 and at unit Reynolds numbers between 8 and 39 million per foot, achieving a maximum of 80% of flight Reynolds numbers across the Mach number range. The test results validated pretest computational fluid dynamic (CFD) simulations of the HWB performance including the OWN benefit and the results also exhibited excellent transonic drag data repeatability to within +/-1 drag count. This paper details the experimental setup and model overview, presents some sample data results, and describes the facility improvements that led to the success of the test.

Chan, David T.

Satellite Sensor Requirements for Monitoring Essential Biodiversity Variables of Coastal Ecosystems

The biodiversity and high productivity of coastal terrestrial and aquatic habitats are the foundation for important benefits to human societies around the world. These globally distributed habitats need frequent and broad systematic assessments, but field surveys only cover a small fraction of these areas. Satellite-based sensors can repeatedly record the visible and near-infrared reflectance spectra that contain the absorption, scattering, and fluorescence signatures of functional phytoplankton groups, colored dissolved matter, and particulate matter near the surface ocean, and of biologically structured habitats (floating and emergent vegetation, benthic habitats like coral, seagrass, and algae). These measures can be incorporated into Essential Biodiversity Variables (EBVs), including the distribution, abundance, and traits of groups of species populations, and used to evaluate habitat fragmentation. However, current and planned satellites are not designed to observe the EBVs that change rapidly with extreme tides, salinity, temperatures, storms, pollution, or physical habitat destruction over scales relevant to human activity. Making these observations requires a new generation of satellite sensors able to sample with these combined characteristics: (1) spatial resolution on the order of 30 to 100-m pixels or smaller; (2) spectral resolution on the order of 5 nm in the visible and 10 nm in the short-wave infrared spectrum (or at least two or more bands at 1,030, 1,240, 1,630, 2,125, and/or 2,260 nm) for atmospheric correction and aquatic and vegetation assessments; (3) radiometric quality with signal to noise ratios (SNR) above 800 (relative to signal levels typical of the open ocean), 14-bit digitization, absolute radiometric calibration less than 2%, relative calibration of 0.2%, polarization sensitivity less than 1%, high radiometric stability and linearity, and operations designed to minimize sunglint; and (4) temporal resolution of hours to days. We refer to these combined specifications as H4 imaging. Enabling H4 imaging is vital for the conservation and management of global biodiversity and ecosystem services, including food provisioning and water security. An agile satellite in a 3-d repeat low-Earth orbit could sample 30-km swath images of several hundred coastal habitats daily. Nine H4 satellites would provide weekly coverage of global coastal zones. Such satellite constellations are now feasible and are used in various applications.

aquatic

An investigation of wing buffeting response at subsonic and transonic speeds. Phase 1: F-111A flight data analysis. Volume 2: Plotted power spectra

Volume 2 of this three volume report is presented. This volume presents plotted variations of power spectral density data with frequency for each structural response item for each data sampled and analyzed during the course of the investigation. Some of the information contained in Volume 1 are repeated to allow the reader to identify the specific conditions appropriate to each plot presented and to interpret the data.

Benepe, D. B.

Comparison of video fields and frames for transform compression

Because of the interlaced television scan, the two fields that form an interlaced video frame are generated 1/60 of a second apart. If the two fields are compressed independently, the correlation between adjacent lines is unused. The transmission rate can be reduced by using a field memory to form an interlaced frame. Four test images were processed as fields and as interlaced frames, using both theoretical and experimental compression designs. For comparable mean-square error and subjective appearance, field compression requires about one-half bit per sample more than frame compression. However, the overall transmission rate - the number of bits per image times the number of images per second - is more meaningful than the number of bits per sample. When transform compression at low transmission rates merges the adjacent lines, frame compression becomes similar to field repeating, and the memory can be reduced.

Jones, H. W., Jr.

Artificial Satellite Analysis Program (ASAP)

Program suited for studying planetary orbit missions including mapping and flyby components. Sample data included for geosynchronous station drift cycle study. Venus radar mapping strategy, frozen orbit about Mars, and repeat ground trace orbit. Written in FORTRAN.

Kwok, Johnny H.

Rapid calibration of seven-hole probes

This paper summarizes the major conclusions and some of the key supporting analyses resulting from the calibration and application of two small seven hole probes at NASA Lewis Research Center. These probes can produce reasonably accurate and rapid surveys of unknown steady flow fields which may include flow angles up to 70 degrees and Mach numbers up to 0.8. The probes were calibrated with both 'complete' and 'reduced' test matrices. Both types of test matrices produced similar results suggesting the the reduced matrices are adequate for most purposes. The average accuracy fo the calibration was about the same as that achieved in previous seven hole probe calibrations. At the higher Mach numbers, the calibration was sensitive to the diameter of the free jet in the calibration facility. Over a narrow angular range at the higher Mach numbers, the system had serious repeatability problems. This lack or repeatability apparently results from aliasing of high frequency (20 to 40 Hz) noise with the data acquisition system sampling frequency of 10 Hz. Analyses show that these noise frequencies are probably not related to airflow dynamics in the connecting tubing.

Ericksen, A. L.

Comparison of Upright Gait with Supine Bungee-Cord Gait

Running on a treadmill with bungee-cord resistance is currently used on the Russian space station MIR as a countermeasure for the loss of bone and muscular strength which occurs during spaceflight. However, it is unknown whether ground reaction force (GRF) at the feet using bungee-cord resistance is similar to that which occurs during upright walking and running on Earth. We hypothesized-that the DRAMs generated during upright walking and running are greater than the DRAMs generated during supine bungee-cord gait. Eleven healthy subjects walked (4.8 +/- 0.13 km/h, mean +/- SE) and ran (9.1 +/- 0.51 km/h) during upright and supine bungee-cord exercise on an active treadmill. Subjects exercised for 3 min in each condition using a resistance of 1 body weight calibrated during an initial, stationary standing position. Data were sampled at a frequency of 500Hz and the mean of 3 trials was analyzed for each condition. A repeated measures analysis of variance tested significance between the conditions. Peak DRAMs during upright walking were significantly greater (1084.9 +/- 111.4 N) than during supine bungee-cord walking (770.3 +/- 59.8 N; p less than 0.05). Peak GRFs were also significantly greater for upright running (1548.3 +/- 135.4 N) than for supine bungee-cord running (1099.5 +/- 158.46 N). Analysis of GRF curves indicated that forces decreased throughout the stance phase for bungee-cord gait but not during upright gait. These results indicate that bungee-cord exercise may not create sufficient loads at the feet to counteract the loss of bone and muscular strength that occurs during long-duration exposure to microgravity.

Boda, Wanda L.