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At least 217 records · Page 12

Hypervelocity Impact Test Results for a Metallic Thermal Protection System

Hypervelocity impact tests have been performed on specimens representing metallic thermal protection systems (TPS) developed at NASA Langley Research Center for use on next-generation reusable launch vehicles (RLV). The majority of the specimens tested consists of a foil gauge exterior honeycomb panel, composed of either Inconel 617 or Ti-6Al-4V, backed with 2.0 in. of fibrous insulation and a final Ti-6Al-4V foil layer. Other tested specimens include titanium multi-wall sandwich coupons as well as TPS using a second honeycomb sandwich in place of the foil backing. Hypervelocity impact tests were performed at the NASA Marshall Space Flight Center Orbital Debris Simulation Facility. An improved test fixture was designed and fabricated to hold specimens firmly in place during impact. Projectile diameter, honeycomb sandwich material, honeycomb sandwich facesheet thickness, and honeycomb core cell size were examined to determine the influence of TPS configuration on the level of protection provided to the substructure (crew, cabin, fuel tank, etc.) against micrometeoroid or orbit debris impacts. Pictures and descriptions of the damage to each specimen are included.

Karr, Katherine L.↗

Issues and Design Drivers for Deep Space Habitats

A cross-disciplinary team of scientists and engineers applied expertise gained in Lunar Lander development to the conceptual design of a long-duration, deep space habitat for Near Earth Asteroid (NEA) missions. The design reference mission involved two launches to assemble a 5-module vehicle for a 380-day round trip mission carrying 4 crew members. The conceptual design process yielded a number of interesting debates, some of which could be significant design drivers in a detailed Deep Space Habitat (DSH) design. These issues include: a) Launch loads: Potentially drives layout of equipment mounted to module floors or walls, and whether temporary internal structure is required to distribute launch loads to minimize shell mass; b) Unmanned loiter time: When added to an already lengthy mission, loiter time further drives risk and reliability, and poses issues for equipment shelf life such as material degradation or cryogenic fluids boil-off; c) Pointing and Visibility: A habitat embedded in a 5-module stack may drive Communications, Tracking, Guidance, and Navigation equipment out onto long booms to maintain line-of-sight visibility with targets. However, long booms will be more susceptible to disruption from exercise-induced vibration, potential damage during docking/undocking operations, and increased power distribution mass; d) Water: although it is assumed that a water processor will collect and recycle water, several interesting question were posed, such as: How much water to start with? Should potable water serve double-duty as radiation protection? And if so, should it be stowed in a single large tank, or smaller, portable containers? e) Design for repairability: one of the worst-case scenarios identified was a cabin depressurization that required suited repair from inside the module, potentially driving the need for long umbilical hoses or special equipment to allow smaller, mated modules to be used as safe havens for up to 180 days;

Anderson, Molly S.↗

Secondary Impacts on Structures on the Lunar Surface

The Altair Lunar Lander is being designed for the planned return to the Moon by 2020. Since it is hoped that lander components will be re-used by later missions, studies are underway to examine the exposure threat to the lander sitting on the Lunar surface for extended periods. These threats involve both direct strikes of meteoroids on the vehicle as well as strikes from Lunar regolith and rock thrown by nearby meteorite strikes. Currently, the lander design is comprised of up to 10 different types of pressure vessels. These vessels included the manned habitation module, fuel, cryogenic fuel and gas storage containers, and instrument bays. These pressure vessels have various wall designs, including various aluminum alloys, honeycomb, and carbon-fiber composite materials. For some of the vessels, shielding is being considered. This program involved the test and analysis of six pressure vessel designs, one of which included a Whipple bumper shield. In addition to the pressure vessel walls, all the pressure vessels are wrapped in multi-layer insulation (MLI). Two variants were tested without the MLI to better understand the role of the MLI in the impact performance. The tests of performed were to examine the secondary impacts on these structures as they rested on the Lunar surface. If a hypervelocity meteor were to strike the surface nearby, it would throw regolith and rock debris into the structure at a much lower velocity. Also, when the manned module departs for the return to Earth, its rocket engines throw up debris that can impact the remaining lander components and cause damage. Glass spheres were used as a stimulant for the regolith material. Impact tests were performed with a gas gun to find the V50 of various sized spheres striking the pressure vessels. The impacts were then modeled and a fast-running approximate model for the V50 data was developed. This model was for performing risk analysis to assist in the vessel design and in the identification of ideal long-term mission sites. This paper reviews the impact tests and analysis and modeling examining the impact threat to various components in the lander design.

Christiansen, Eric↗

An Approach to Solving Enclosure Radiation Problems in A Multi-Physics Context

Thermal protection system analysis of complex features or damage sites can sometimes require modeling of high temperature enclosures. Implementing efficient and accurate view-factor algorithms required to model such problems is complex. The current work leverages the Non-equilibrium Radiation (NERO) software, which solves the radiation transport equation in a finite-volume scheme, to alleviating challenges often faced with view-factor calculations. By assuming heat transfer occurs only between grey bodies and that the medium is non-participating, computational cost of the method is significantly reduced. The enclosure physics are modeled through emitting and reflecting boundary conditions in NERO. The emitted radiative flux is dependent on the wall temperature which is a solution to the material response, obtained from Icarus, in this context. The Ares framework manages the time-advancement and exchange of the necessary data between the solvers. The surface energy balance is modified to account for the enclosure terms within the material response boundary condition. The methodology was verified against analytical solutions including radiating parallel plates, a hollow cylinder (shown in Fig. 1), and a hemisphere. Application of the methodology to inform the design of components of the Dragonfly system will be shown.

Ablation↗

Designing the Next Generation of Human Spacecraft

Lunar Space Station Common Module: A new concept for a module for a lunar space station attempts to reduce the module's mass by abandoning the traditional rack structure currently used on the ISS for the mounting of internal hardware and replacing it with a core structure. By using this design, the pressure shell will not have to carry the loads resulting from the internal mass. I worked with another intern to create the initial design for the module, with him focusing on the core and myself focusing on the pressure shell. To start, I was given the shell overall dimensions and material and tasked with sizing the wall thickness and placing stiffeners such that the shell could withstand the required loads. At the same time, I had to keep the mass to a minimum to keep the overall module within the allowable launch mass. Once I had done initial sizing based on pressure loads, I combined the pressure shell with the inner core to perform optimization of the design. Currently, the design involves circumferential stiffeners along the entire length of the pressure shell with longitudinal stiffeners on either end. In addition, extra wall thickness was added around each of the hatches. At this stage, the design shows a comparable mass to a more traditional design, but we are hopeful that, through optimization, we will be able to reduce the mass even further. There is currently a patent pending for the module design, for which I am listed as a co-inventor. ALON Material Testing: I was given samples of aluminum oxynitride (ALON) that had been impacted by a previous intern on which to perform residual strength tests as part of a plan to approve them for space use. Before testing, I measured the pucks and their damages using a ruler and optical micrometer in order to verify that the puck dimensions were within the tolerances set by the test guidelines and that the damages had not grown when the pucks were thinned. The test was a ring-ring test, which used two concentric rings to place the ring in axisymmetric bending, with the puck set up so that the damaged side was always in tension. Though I was unable to do the setup of the test or run the load machine due to a period of changing test procedures, I was able to observe the testing and perform the data collection. The pucks behaved as expected, breaking at the damage, as did the strengths calculated from the data, being lower than for the unimpacted pucks and having less scatter between the puck values. The attached image is of myself during the ALON strength testing. Over the course of my internship, I was able to learn much more about real-life structural analysis and about the behavior of materials, and it confirmed my previous interest in structural analysis. At the same time, due to the opportunities offered to interns, I was able to learn a lot about mission control, and, in doing so, I developed a second interest in working in mission control. In addition, being able to meet the people here and learn about the type of work NASA does made me want to come back to work for NASA full time.

Simmons, Emily↗

Bioreactors Drive Advances in Tissue Engineering

It was an unlikely moment for inspiration. Engineers David Wolf and Ray Schwarz stopped by their lab around midday. Wolf, of Johnson Space Center, and Schwarz, with NASA contractor Krug Life Sciences (now Wyle Laboratories Inc.), were part of a team tasked with developing a unique technology with the potential to enhance medical research. But that wasn t the focus at the moment: The pair was rounding up colleagues interested in grabbing some lunch. One of the lab s other Krug engineers, Tinh Trinh, was doing something that made Wolf forget about food. Trinh was toying with an electric drill. He had stuck the barrel of a syringe on the bit; it spun with a high-pitched whirr when he squeezed the drill s trigger. At the time, a multidisciplinary team of engineers and biologists including Wolf, Schwarz, Trinh, and project manager Charles D. Anderson, who formerly led the recovery of the Apollo capsules after splashdown and now worked for Krug was pursuing the development of a technology called a bioreactor, a cylindrical device used to culture human cells. The team s immediate goal was to grow human kidney cells to produce erythropoietin, a hormone that regulates red blood cell production and can be used to treat anemia. But there was a major barrier to the technology s success: Moving the liquid growth media to keep it from stagnating resulted in turbulent conditions that damaged the delicate cells, causing them to quickly die. The team was looking forward to testing the bioreactor in space, hoping the device would perform more effectively in microgravity. But on January 28, 1986, the Space Shuttle Challenger broke apart shortly after launch, killing its seven crewmembers. The subsequent grounding of the shuttle fleet had left researchers with no access to space, and thus no way to study the effects of microgravity on human cells. As Wolf looked from Trinh s syringe-capped drill to where the bioreactor sat on a workbench, he suddenly saw a possible solution to both problems. It dawned on me that rotating the wall of the reactor would solve one of our fundamental fluid mechanical problems, specifically by removing the velocity gradient of the tissue culture fluid media near the reactor s walls, says Wolf. It looked as though it would allow us to suspend the growing cells within the reactor without introducing turbulent fluid mechanical conditions.

Source record↗

Composite wall concept for high temperature turbine shrouds: Survey of low modulus strain isolator materials

Plasma sprayed yttria stabilized zirconium oxide turbine seal specimens, incorporating various low modulus porous metal strain isolator pads between the zirconium oxide and a dense metal substrate, were subjected to cyclic thermal shock testing. Specimens that had a low modulus pad composed of sintered FeNiCrAlY fibermetal survived 1000 thermal shock cycles without spalling of the ceramic. A figure of merit for the low modulus pad materials taking into consideration the elastic modulus, thermal conductivity, strength, and oxidation resistance of the pad was proposed, and showed reasonable agreement with the thermal shock results. A potential surface distress problem on the zirconium oxide, associated with nonuniform temperature distribution and rapid stress relaxation was identified. One approach to solving the surface distress problem through application of laser surface fusion of the zirconium oxide layer showed some promise, but improvements in the laser surface fusion process are necessary to prevent process associated damage to the ceramic.

Bill, R. C.↗

Development of an Aeroelastic Modeling Capability for Transient Nozzle Side Load Analysis

Lateral nozzle forces are known to cause severe structural damage to any new rocket engine in development. Currently there is no fully coupled computational tool to analyze this fluid/structure interaction process. The objective of this study was to develop a fully coupled aeroelastic modeling capability to describe the fluid/structure interaction process during the transient nozzle operations. The aeroelastic model composes of three components: the computational fluid dynamics component based on an unstructured-grid, pressure-based computational fluid dynamics formulation, the computational structural dynamics component developed in the framework of modal analysis, and the fluid-structural interface component. The developed aeroelastic model was applied to the transient nozzle startup process of the Space Shuttle Main Engine at sea level. The computed nozzle side loads and the axial nozzle wall pressure profiles from the aeroelastic nozzle are compared with those of the published rigid nozzle results, and the impact of the fluid/structure interaction on nozzle side loads is interrogated and presented.

Wang, Ten-See↗

System for Centering a Turbofan in a Nacelle During Tests

A feedback position-control system has been developed for maintaining the concentricity of a turbofan with respect to a nacelle during acoustic and flow tests in a wind tunnel. The system is needed for the following reasons: Thermal and thrust loads can displace the fan relative to the nacelle; In the particular test apparatus (see Figure 1), denoted as a rotor-only nacelle (RAN), the struts, vanes, and other stator components of a turbofan engine that ordinarily maintain the required concentricity in the face of thermal and thrust loads are not present; and The struts and stator components are not present because it is necessary to provide a flow path that is acoustically clean in the sense that the measured noise can be attributed to the fan alone. The system is depicted schematically in Figure 2. The nacelle is supported by two struts attached to a two-axis traverse table located outside the wind-tunnel wall. Two servomotors acting through 100:1 gearboxes drive the table along the Y and Z axes, which are perpendicular to the axis of rotation. The Y and Z components of the deviation from concentricity are measured by four laser displacement sensors mounted on the nacelle and aimed at reflective targets on the center body, which is part of the fan assembly. The outputs of the laser displacement sensors are digitized and processed through a personal computer programmed with control software. The control output of the computer commands the servomotors to move the table as needed to restore concentricity. Numerous software and hardware travel limits and alarms are provided to maximize safety. A highly ablative rub strip in the nacelle minimizes the probability of damage in the event that a deviation from concentricity exceeds the radial clearance [<0.004 in. (<0.1 mm)] between the inner surface of the nacelle and the tips of the fan blades. To be able to prevent an excursion in excess of the tip clearance, the system must be accurate enough to control X and Y displacements to within 0.001 in. (.0.025 mm). One characteristic essential to such accuracy is sufficient rigidity in the mechanical components of the system to prevent excitation of vibrations in the strut/ nacelle subsystem. The need for such a high degree of accuracy prompted a comprehensive analysis of sources of measurement and control errors, followed by rigorous design efforts to minimize these errors. As a result, the design of the system incorporates numerous improvements in hardware, software, and operational procedures.

Cunningham, Cameron C.↗

Assessing the Effects of Various Surface Textures and Features on Turbulent Heat Transfer in Hypersonic Flight

Experiments were conducted in the NASA Ames Hypervelocity Free Flight Aerodynamic Facility (ballistic range) to quantify the effects on turbulent convective heat transfer of distributed surface roughness, and of isolated features, representative of thermal protection systems on atmospheric entry vehicles. The surface textures and features were applied on the conic frusta of 45o sphere-cone models having a nose-to-base radius ratio of 0.5, similar to the forebody geometry of the Galileo and Pioneer-Venus entry probes. Test conditions were selected to provide turbulent roughness Reynolds numbers, k+, in the ranges expected for outer planet entry missions. Turbulent flow on the conic frustum was achieved by tripping the flow on the sphere-segment nose cap with distributed surface roughness, created by sand-blasting the nose cap. Surface textures included distributed, acreage, roughness, as well as cavity and groove discrete features. The majority of the data to be presented are results for distributed roughness, which includes both random, sand-grain-like roughness, and regular pattern roughness. The pattern roughness was designed to represent the roughness on woven thermal protection system materials, such as NASA’s 3-D Medium Density Carbon Phenolic (3MDCP), also known as HEEET, developed by the Heatshield for Extreme Entry Environments Technology project. The pattern roughness tested is a 3-D wavy surface, and includes three different roughness element height-to-spacing ratios representative of two configurations of 3MDCP, and spanning ratios measured both before, and after, ablation in an arc jet test facility. The patterns tested in the ballistic range were laser-etched on metal models, and represented an idealized version of the real-world materials in which each roughness element was nearly identical. Additional tests were performed wherein the laser-etched patterns were degraded by sand-blasting with various sized grit media, to produce regular patterns with superimposed irregular roughness, more representative of flight materials. Results of each will be compared. The discrete features tested included cylindrical cavities and rectangular grooves of various width-to-depth ratios. Cavities represent either heatshield damage, such as from micro-meteoroid and orbital debris (MMOD) damage, or from designed penetrations, such as on the Genesis sample return capsule. The grooves were scaled representations of seams between segments of HEEET material in a notional tiled thermal protection system. The tests examined the effects on turbulent heating downstream of the isolated features. The tests were conducted at speeds between 3 km/s and 4 km/s in air between 0.15 atm and 0.25 atm (Mach numbers between 9 and 12). Roughness Reynolds numbers, k+, ranged from 12 to 70 for the sand roughness, and as high as 200 for the pattern roughness. Boundary-layer parameters required for calculating k+ were evaluated using computational fluid dynamics simulations using the DPLR (Data Parallel Line Relaxation) code. Each model included both rough- and smooth-wall segments, and heat transfer augmentation factors were determined as the ratio of the rough-wall to smooth-wall heat flux measured on each test.

hypersonic↗

Impact of composite plates: Analysis of stresses and forces

The foreign object damage resistance of composite fan blades was studied. Edge impact stresses in an anisotropic plate were first calculated incorporating a constrained layer damping model. It is shown that a very thin damping layer can dramatically decrease the maximum normal impact stresses. A multilayer model of a composite plate is then presented which allows computation of the interlaminar normal and shear stresses. Results are presented for the stresses due to a line impact load normal to the plane of a composite plate. It is shown that significant interlaminar tensile stresses can develop during impact. A computer code was developed for this problem using the fast Fourier transform. A marker and cell computer code were also used to investigate the hydrodynamic impact of a fluid slug against a wall or turbine blade. Application of fluid modeling of bird impact is reviewed.

Moon, F. C.↗

Facility and Methods Developed for Simulated Space Vacuum Ultraviolet Exposure Testing of Polymer Films

Vacuum ultraviolet (VUV) radiation of wavelengths between 115 and 200 nm produced by the Sun in the space environment can degrade polymer films, producing changes in their optical, mechanical, and chemical properties. These effects are particularly important for thin polymer films being considered for ultralightweight space structures, because, for most polymers, VUV radiation is absorbed in a thin surface layer. The NASA Glenn Research Center has developed facilities and methods for long-term ground testing of polymer films to evaluate space environmental VUV radiation effects. VUV exposure can also be used as part of combined or sequential simulated space environmental exposures to determine combined damaging effects with other aspects of the space environment, which include solar ultraviolet radiation, solar flare x-rays, electron and proton radiation, atomic oxygen (for low-Earth-orbit missions), and temperature effects. Because the wavelength sensitivity of VUV damage is not well known for most materials, Glenn's VUV facility uses a broad-spectrum deuterium lamp with a magnesium fluoride window that provides output between 115 and 200 nm. Deuterium lamps of this type were characterized by the National Institute of Standards and Technology and through measurements at Glenn. Spectral irradiance measurements show that from approximately 115 to 160 nm, deuterium lamp irradiance can be many times that of air mass zero solar irradiance, and as wavelength increases above approximately 160 nm, deuterium lamp irradiance decreases in comparison to the Sun. The facility is a cryopumped vacuum chamber that achieves a system pressure of approximately 5310(exp -6) torr. It contains four individual VUV-exposure compartments in vacuum, separated by water-cooled copper walls to minimize VUV radiation and any sample contamination cross interactions between compartments. Each VUV-exposure compartment contains a VUV deuterium lamp, a motor-controlled sample stage coupled with a moveable cesium iodide VUV phototube, and two thermocouples for temperature measurement. The vacuum chamber and exterior equipment is shown. Each VUV lamp is located at the top of the chamber with its projection-tube pushed through an O-ring compression fitting. The lamp assemblies are located on ports that can be isolated from the rest of the vacuum chamber, permitting maintenance or replacement of the lamps without breaking vacuum in the main chamber where the samples are located. A view of two of the four interior VUV-exposure compartments, including the moveable sample stages and detector holders is also shown. Glenn is using this facility to support testing of Next Generation Space Telescope sunshield materials that is being led by the NASA Goddard Space Flight Center and to develop an understanding of the wavelength, intensity, and temperature dependence of VUV-induced polymer degradation.

Dever, Joyce A.↗

Near Real-Time Flood Monitoring and Impact Assessment Systems

Floods are the costliest natural disaster, causing approximately 6.8 million deaths in the twentieth century alone. Worldwide economic flood damage estimates in 2012 exceed $19 Billion USD. Extended duration floods also pose longer term threats to food security, water, sanitation, hygiene, and community livelihoods, particularly in developing countries. Projections by the Intergovernmental Panel on Climate Change (IPCC) suggest that precipitation extremes, rainfall intensity, storm intensity, and variability are increasing due to climate change. Increasing hydrologic uncertainty will likely lead to unprecedented extreme flood events. As such, there is a vital need to enhance and further develop traditional techniques used to rapidly assess flooding and extend analytical methods to estimate impacted population and infrastructure. Measuring flood extent in situ is generally impractical, time consuming, and can be inaccurate. Remotely sensed imagery acquired from space-borne and airborne sensors provides a viable platform for consistent and rapid wall-to-wall monitoring of large flood events through time. Terabytes of freely available satellite imagery are made available online each day by NASA, ESA, and other international space research institutions. Advances in cloud computing and data storage technologies allow researchers to leverage these satellite data and apply analytical methods at scale. Repeat-survey earth observations help provide insight about how natural phenomena change through time, including the progression and recession of floodwaters. In recent years, cloud-penetrating radar remote sensing techniques (e.g., Synthetic Aperture Radar) and high temporal resolution imagery platforms (e.g., MODIS and its 1-day return period), along with high performance computing infrastructure, have enabled significant advances in software systems that provide flood warning, assessments, and hazard reduction potential. By incorporating social and economic data, researchers can develop systems that automatically quantify the socioeconomic impacts resulting from flood disaster events.

Ahamed, Aakash↗

Monte Carlo track structure for radiation biology and space applications

Over the past two decades event by event Monte Carlo track structure codes have increasingly been used for biophysical modelling and radiotherapy. Advent of these codes has helped to shed light on many aspects of microdosimetry and mechanism of damage by ionising radiation in the cell. These codes have continuously been modified to include new improved cross sections and computational techniques. This paper provides a summary of input data for ionizations, excitations and elastic scattering cross sections for event by event Monte Carlo track structure simulations for electrons and ions in the form of parametric equations, which makes it easy to reproduce the data. Stopping power and radial distribution of dose are presented for ions and compared with experimental data. A model is described for simulation of full slowing down of proton tracks in water in the range 1 keV to 1 MeV. Modelling and calculations are presented for the response of a TEPC proportional counter irradiated with 5 MeV alpha-particles. Distributions are presented for the wall and wall-less counters. Data shows contribution of indirect effects to the lineal energy distribution for the wall counters responses even at such a low ion energy.

NASA Center JSC↗

A Study of the Swelling and Bursting of the High Pressure Coupling (HPC) of a Two-Stage Light Gas Gun

• Need for data at muzzle velocities of 7.0 km/s and above to simulate impacts of space debris and naturally occurring meteoroids • The two-stage light gas gun is a well known way of achieving these velocities • There is a danger of significant swelling and even bursting of the HPC (high pressure coupling). • A consistent picture of the swelling and bursting of the HPCs of two Ames two-stage light gas guns is developed, based on the following: 1. Experimental measurements of the swelling and bursting of the HPCs of two two-stage light gas guns. 2. Stress-strain calculations of the swelling and bursting of thick walled steel tubes. 3. Experimental measurements swelling and bursting of thick walled steel tubes. 4. CFD calculations of the maximum pressures in the HPC of a two-stage light gas gun using the code LGGUN. • It is shown that model developed predicts swelling and bursting that agrees reasonably well with measured data. • Hence, it is suggested that the model could be used in the design of a two-stage light gas guns to assess the risk of swelling and bursting and to give maximum suggested operating conditions for an existing gun.

Ballistic Range↗

Space-Spurred Metallized Materials

Spurred R&D toward improved vacuum metallizing techniques led to an extensive line of commercial products, from insulated outdoor garments to packaging for foods, from wall coverings to window shades, from life rafts to candy wrappings, reflective blankets to photographic reflectors. Metallized Products, Inc. (MPI) was one of the companies that worked with NASA in development of the original space materials. MPI markets its own metallized products and supplies materials to other manufacturers. One of the most widely used MPI products is TXG laminate. An example is a reflective kite, the S.O.S. Signal Kite that can be flown as high as 200 feet to enhance radar and visual detectability. It offers a boon to campers, hikers, mountain climbers and boaters. It is produced by Solar Reflections, Inc. The company also markets a solar reflective hat. Another example is by Pro-Tektion, Inc. to provide protection for expensive musical equipment that have sensitive electronic components subject to damage from the heat of stage lights, dust, or rain at outdoor concerts. MP supplied the material and acceptance of the covers by the sound industry has been excellent.

Source record↗

A computational study of the impingement region of an unsteady subsonic jet

Vertical/Short Take-Off and Landing (V/STOL) aircraft may encounter operating difficulties while hovering near the ground. Lift loss, hot gas and debris ingestion, and landing surface damage are some of the potential problems which can result from the interaction of the propulsive jets with the airframe and ground. The purpose of the present work is to develop a detailed understanding of a flowfield, a jet exiting from a flat plate impacting a ground plane, that contains much of the essential physics of the complex flows encountered by V/STOL aircraft in hover. To achieve this, a high-resolution time-accurate numerical simulation of the impinging jet flow was performed. The numerical simulation is based on the solution of the laminar compressible Navier-Stokes equations using the Beam-Warming algorithm. Complex start-up and periodic jet and jet-induced vortex structures were captured, including the formation of primary and secondary jet-induced vortices and wall-jet separation. The formation and development of these phenomena are supported by experimental measurements and smoke visualization. Results are compared with appropriate experiments.

Jalamani, Zakaria A.↗

Tissuelike 3D Assemblies of Human Broncho-Epithelial Cells

Three-dimensional (3D) tissuelike assemblies (TLAs) of human broncho-epithelial (HBE) cells have been developed for use in in vitro research on infection of humans by respiratory viruses. The 2D monolayer HBE cell cultures heretofore used in such research lack the complex cell structures and interactions characteristic of in vivo tissues and, consequently, do not adequately emulate the infection dynamics of in-vivo microbial adhesion and invasion. In contrast, the 3D HBE TLAs are characterized by more-realistic reproductions of the geometrical and functional complexity, differentiation of cells, cell-to-cell interactions, and cell-to-matrix interactions characteristic of human respiratory epithelia. Hence, the 3D HBE TLAs are expected to make it possible to perform at least some of the research in vitro under more-realistic conditions, without need to infect human subjects. The TLAs are grown on collagen-coated cyclodextran microbeads under controlled conditions in a nutrient liquid in the simulated microgravitational environment of a bioreactor of the rotating- wall-vessel type. Primary human mesenchymal bronchial-tracheal cells are used as a foundation matrix, while adult human bronchial epithelial immortalized cells are used as the overlying component. The beads become coated with cells, and cells on adjacent beads coalesce into 3D masses. The resulting TLAs have been found to share significant characteristics with in vivo human respiratory epithelia including polarization, tight junctions, desmosomes, and microvilli. The differentiation of the cells in these TLAs into tissues functionally similar to in vivo tissues is confirmed by the presence of compounds, including villin, keratins, and specific lung epithelium marker compounds, and by the production of tissue mucin. In a series of initial infection tests, TLA cultures were inoculated with human respiratory syncytial viruses and parainfluenza type 3 viruses. Infection was confirmed by photomicrographs that showed signs of damage by viruses and virus titers (see figure) that indicated large increases in the populations of viruses during the days following inoculation.

Goodwin, Thomas J.↗