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At least 235 records · Page 13

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NASA NTRS2012

Molecular Technique to Reduce PCR Bias for Deeper Understanding of Microbial Diversity

Current planetary protection policies require that spacecraft targeted to sensitive solar system bodies be assembled and readied for launch in controlled cleanroom environments. A better understanding of the distribution and frequency at which high-risk contaminant microbes are encountered on spacecraft surfaces would significantly aid in assessing the threat of forward contamination. However, despite a growing understanding of the diverse microbial populations present in cleanrooms, less abundant microbial populations are probably not adequately taken into account due to technological limitations. This novel approach encompasses a wide spectrum of microbial species and will represent the true picture of spacecraft cleanroom-associated microbial diversity. All of the current microbial diversity assessment techniques are based on an initial PCR amplification step. However, a number of factors are known to bias PCR amplification and jeopardize the true representation of bacterial diversity. PCR amplification of a minor template appears to be suppressed by the amplification of a more abundant template. It is widely acknowledged among environmental molecular microbiologists that genetic biosignatures identified from an environment only represent the most dominant populations. The technological bottleneck overlooks the presence of the less abundant minority population and may underestimate their role in the ecosystem maintenance. DNA intercalating agents such as propidium monoazide (PMA) covalently bind with DNA molecules upon photolysis using visible light, and make it unavailable for DNA polymerase enzyme during polymerase chain reaction (PCR). Environmental DNA samples will be treated with suboptimum PMA concentration, enough to intercalate with 90 99% of the total DNA. The probability of PMA binding with DNA from abundant bacterial species will be much higher than binding with DNA from less abundant species. This will increase the relative DNA concentration of previously "shadowed" less abundant species available for PCR amplification. These PCR products obtained with and without PMA treatment will then be subjected to downstream diversity analyses such as sequencing and DNA microarray. It is expected that PMA-coupled PCR will amplify the "minority population" and help in understanding microbial diversity spectrum of an environmental sample at a much deeper level. This new protocol aims to overcome the major potential biases faced when analyzing microbial 16S rRNA gene diversity. This study will lead to a technological advancement and a commercial product that will aid microbial ecologists in understanding microbial diversity from various environmental niches. Implementation of this technique may lead to discoveries of novel microbes and their functions in sustenance of the ecosystem.

Vaishampayan, Parag A.↗

NASA NTRS2021

Hypersonic Boundary-Layer Transition on Blunted Cones at Angle of Attack

Experimental studies of cones at several high-speed facilities have demonstrated that, for small nosetip bluntness, transition onset over a circular cone moves upstream along the leeward side and downstream along the windward side, but this trend may be reversed at large bluntness values, where transition onset moves downstream along the leeward side and upstream along the windward side. A theoretical and numerical investigation is performed to characterize the effects of nose bluntness on disturbance amplification over the circular cone for several angles of attack, with the goal of understanding the potential physical mechanisms behind the experimental observations. The three-dimensional laminar basic states over a 1.5 m long, 7-degree half-angle cone with 9.525 mm nosetip radius are computed for selected angles of attack values and freestream conditions that are selected to match the Mach 10 experiments conducted within the Hypervelocity Wind Tunnel 9 at the Arnold Engineering Development Complex (AEDC). The solutions at a freestream unit Reynolds number of 17.1 million per meter are used to perform detailed instability analyses for angles of attack equal to 0, 1, 3, and 5 degrees. Results indicate that the linear amplification of stationary crossflow waves along inflection lines may begin to influence transition along the acreage of the cone for angles of attack equal to or larger than 5 degrees. The measured trend in transition front with respect to increasing angle of attack is found to be consistent with the predicted increase in the amplification factors for Mack mode disturbances along the streamline trajectories. The increase in Mack mode amplification along the windward ray for higher angles of attack is shown to be the result of a progressively earlier entropy-layer swallowing. Computations also indicate that the transition amplification factor along the windward ray is not constant and increases with the angle of attack and that the transition #-factors along the leeward ray are rather small. The nonmodal analysis for zero degrees angle of attack shows that entropy-layer disturbances with appreciably strong energy growth can coexist with Mack mode instabilities at the measured transition location.

boundary layer transition↗

NASA NTRS2021

Hypersonic Boundary-Layer Transition on Blunted Cones at Angle of Attack

Boundary Layer Transition↗

NASA NTRS1979

On the stability of the boundary layer on a transonic swept wing

Both incompressible and compressible linear stability theory are applied to the three-dimensional compressible boundary layer on a particular transonic sweptback wing of infinite span. A spatial stability theory is used which identifies the growth direction with the real part of the complex angle of the group velocity. It is found that in the forward, but not the rear, crossflow instability region, the maximum amplification rates of the steady disturbances may be calculated to within about 10% by the incompressible stability theory. There is little difference between the sixth and eighth-order compressible theories. The maximum amplification rate of the steady disturbances at any chordwise station is closely related to the maximum crossflow at that station independent of the Reynolds number. For other than crossflow instability, there can be large differences between the incompressible and compressible theories, both as to the amplification rate and the angle of the wavenumber vector for maximum instability. Amplitude ratios of individual wave components are obtained by integrating the spatial amplification rate along the growth direction subject to the constraint that the wavenumber vector is irrotational. This procedure yields steady disturbances aligned with the local potential flow direction whose wavelengths are nearly independent of downstream distance.

Mack, L. M.↗

NASA NTRS1979

Analysis of flight effects on noise radiation from dual-flow coaxial jets

A theoretical study has been made of the effects of flight on noise from dual-flow coaxial jets. The theory is based on an instability free, vortex sheet flow model. It is shown that the flight effects are more favorable (and hence produce less forward-arc amplification) for coaxial jets than for single-stream jets. Further, the theory predicts that, like the single-stream jet case, flight effects induce noise amplification in the forward quadrant and attenuation in the aft quadrant and have virtually no effect at theta = 90 deg to the jet axis, where theta is the angle between the directions of convection and emission at the retarded time. Amplification in the forward quadrant diminishes as the inner flow velocity increases and becomes optimum when the outer-to-inner velocity ratio is about 0.5. The theory also shows that the higher the outer-to-inner area ratio, the lower the forward-arc amplification due to flight.

Dash, R.↗

NASA NTRS1981

Compressible boundary-layer stability calculations for sweptback wings with suction

The stability of the laminar boundary layers on two transonic wings of infinite span with distributed suction is investigated with the compressible, parallel-flow stability theory. Both wings have supercritical airfoil sections; one has a sweep angle of 23 deg, the other of 35 deg. Zero-frequency disturbances are used to represent cross-flow instability, and disturbances with the wavenumber vector aligned with the local flow direction represent traveling-wave instability. In both cases, the maximum spatial amplification rate is used as a measure of the instability. For the suction, distributions with constant mass flux downstream of the starting point are used. The main objective is to determine how the maximum amplification rate varies with the magnitude and starting point of the suction. It is found for both types of disturbances that the maximum amplification rate varies almost linearly with the suction magnitude up to at least the point where the amplification rate is halved. Different starting locations for the suction in the first 4% of the chord were found to affect cross-flow instability, but to have little influence on traveling-wave instability.

Mack, L. M.↗

NASA NTRS1984

Large-scale coherent structure and far-field jet noise

The phenomenon of broadband noise amplification/suppression under controlled excitation is investigated. The suppression is found to occur only at low jet speeds when the exit boundary layer is laminar; excitation at a Strouhal St(theta) number of about 0.017 results in optimum suppression of the far-field broadband noise as well as of the near-flow-field turbulence. The noise amplification is found to be a function of the Mach number, the amplification is higher at higher Mach number for a given excitation Strouhal number and level. The amplification also depends on the Strouhal number (St D), the maximum occurring in the range 0.65-0.85. Vortex pairing induced by the excitation appears to be at the heart of the noise amplification phenomenon under excitation.

Zaman, K. B. M. Q.↗

NASA NTRS1986

Natural laminar flow flight experiments on a swept wing business jet-boundary layer stability analyses

The linear boundary layer stability analyses and their correlation with data of 18 cases from a natural laminar flow (NLF) flight test program using a Cessna Citation 3 business jet are described. The transition point varied from 5% to 35% chord for these conditions, and both upper and lower wing surfaces were included. Altitude varied from 10,000 to 43,000 ft and Mach number from 0.3 to 0.8. Four cases were at nonzero sideslip. Although there was much scatter in the results, the analyses of boundary layer stability at the 18 conditions led to the conclusion that crossflow instability was the primary cause of transition. However, the sideslip cases did show some interaction of crossflow and Tollmien-Schlichting disturbances. The lower surface showed much lower Tollmien-Schlichting amplification at transition than the upper surface, but similar crossflow amplifications. No relationship between Mach number and disturbance amplification at transition could be found. The quality of these results is open to question from questionable wing surface quality, inadequate density of transition sensors on the wing upper surface, and an unresolved pressure shift in the wing pressure data. The results of this study show the need for careful preparation for transition experiments. Preparation should include flow analyses of the test surface, boundary layer disturbance amplification analyses, and assurance of adequate surface quality in the test area. The placement of necessary instruments and usefulness of the resulting data could largely be determined during the pretest phase.

Rozendaal, R. A.↗

NASA NTRS1987

Theory of plasma contactors used in the ionosphere

The use of plasma contactors has been proposed as a means of enhancing the current flow through an electrodynamic tether. A simple isothermal spherical model of the plasma cloud around a contactor is outlined for a plasma contactor which is biased positively with respect to the ambient plasma and hence collects electrons. It is shown that for significant current amplification to occur, the plasma cloud must be turbulent. The amount of current amplification is obtained as a function of the ion current through the contactor. For ion currents of several amperes amplification factors of 2-6 can be obtained for potential drops in the range 100-500 V. For smaller ion currents, much larger amplification factors can be obtained.

Hastings, D. E.↗

NASA NTRS1987

The doubled CO2 climate - Impact of the sea surface temperature gradient

The Goddard Institute for Space Studies (GISS) GCM of Hansen et al. (1983) was run, with 4 deg x 5 deg resolution, with doubled CO2 and two sets of sea surface temperature gradient distributions. One set was derived from the equilibrium doubled CO2 run of the 8 deg x 10 deg GISS GCM, with minimal high latitude amplification. The other set resembled closely the GFDL model results, with greater amplification. Both experiments had the same global mean surface air temperature change. The two experiments were often found to produce substantially different climate characteristics. With reduced high latitude amplification (set one), and thus, more equatorial warming, there was a greater increase in specific humidity and the greenhouse capacity of the atmosphere, resulting in a warmer atmosphere in general. Features such as the low-latitude precipitation, Hadley cell intensity, jet stream magnitude, and atmospheric energy transports all increased in comparison with the control run. In contrast, these features all decreased in the experiment with greater high latitude amplification (set two).

Rind, David↗

NASA NTRS1988

Gravitational lensing statistics of amplified supernovae

Amplification statistics of gravitationally lensed supernovae can provide a valuable probe of the lensing matter in the universe. A general probability distribution for amplification by compact objects is derived which allows calculation of the lensed fraction of supernovae at or greater than an amplification A and at or less than an apparent magnitude. Comparison of the computed fractions with future results from ongoing supernova searches can lead to determination of the mass density of compact dark matter components with masses greater than about 0.001 solar mass, while the time-dependent amplification (and polarization) of the expanding supernovae constrain the individual masses. Type II supernovae are found to give the largest fraction for deep surveys, and the optimum flux-limited search is found to be at approximately 23d magnitude, if evolution of the supernova rate is neglected.

Linder, Eric V.↗

NASA NTRS1988

Light propagation in inhomogeneous universes

Refsdal's (1970) method is generalized to study the propagation of light rays through an inhomogeneous universe. The probability distribution for the linear component of the cumulative shear (CS) along light rays is derived, and it is shown that the CS can be dominated by nonlinear components, espcially for light rays in empty cones. The amplification tail of the amplification probability distribution is compared with analytic results; these linear investigations are shown to underestimate the high-amplification probability and hence the importance of the amplification bias in source counts. The distribution of the ellipticity of images of infinitesimal circular sources is derived, and it is shown that this can be dominated by the nonlinear contributions to the CS.

Schneider, Peter↗

NASA NTRS1992

Ideal photon number amplifier and duplicator

The photon number-amplification and number-duplication mechanism are analyzed in the ideal case. The search for unitary evolutions leads to consider also a number-deamplification mechanism, the symmetry between amplification and deamplification being broken by the integer-value nature of the number operator. Both transformations, amplification and duplication, need an auxiliary field which, in the case of amplification, turns out to be amplified in the inverse way. Input-output energy conservation is accounted for using a classical pump or through frequency-conversion of the fields. Ignoring one of the fields is equivalent to considering the amplifier as an open system involving entropy production. The Hamiltonians of the ideal devices are given and compared with those of realistic systems.

Dariano, G. M.↗

NASA NTRS1991

Effect of Compliant Walls on Secondary Instabilities in Boundary-Layer Transition

For aerodynamic and hydrodynamic vehicles, it is highly desirable to reduce drag and noise levels. A reduction in drag leads to fuel savings. In particular for submersible vehicles, a decrease in noise levels inhibits detection. A suggested means to obtain these reduction goals is by delaying the transition from laminar to turbulent flow in external boundary layers. For hydrodynamic applications, a passive device which shows promise for transition delays is the compliant coating. In previous studies with a simple mechanical model representing the compliant wall, coatings were found that provided transition delays as predicted from the semi-empirical e(sup n) method. Those studies were concerned with the linear stage of transition where the instability of concern is referred to as the primary instability. For the flat-plate boundary layer, the Tollmien-Schlichting (TS) wave is the primary instability. In one of those studies, it was shown that three-dimensional (3-D) primary instabilities, or oblique waves, could dominate transition over the coatings considered. From the primary instability, the stretching and tilting of vorticity in the shear flow leads to a secondary instability mechanism. This has been theoretical described by Herbert based on Floquet theory. In the present study, Herbert's theory is used to predict the development of secondary instabilities over isotropic and non-isotropic compliant walls. Since oblique waves may be dominant over compliant walls, a secondary theory extention is made to allow for these 3-D primary instabilities. The effect of variations in primary amplitude, spanwise wavenumber, and Reynolds number on the secondary instabilities are examined. As in the rigid wall case, over compliant walls the subharmonic mode of secondary instability dominates for low-amplitude primary disturbances. Both isotropic and non-isotropic compliant walls lead to reduced secondary growth rates compared to the rigid wall results. For high frequencies, the non-isotropic wall suppresses the amplification of the secondary instabilities, while instabilities over the isotropic wall may grow with an explosive rate similar to the rigid wall results. For the more important lower frequencies, both isotropic and non-isotropic compliant walls suppress the amplification of secondary instabilities compared to the rigid wall results. The twofold major discovery and demonstration of the present investigation are: (1) the use of passive devices, such as compliant walls, can lead to significant reductions in the secondary instability growth rates and amplification; (2) suppressing the primary growth rates and subsequent amplification enable delays in the growth of the explosive secondary instability mechanism.

Joslin, Ronald D.↗

NASA NTRS1994

Emergence of a replicating species from an in vitro RNA evolution reaction

The technique of self-sustained sequence replication allows isothermal amplification of DNA and RNA molecules in vitro. This method relies on the activities of a reverse transcriptase and a DNA-dependent RNA polymerase to amplify specific nucleic acid sequences. We have modified this protocol to allow selective amplification of RNAs that catalyze a particular chemical reaction. During an in vitro RNA evolution experiment employing this modified system, a unique class of "selfish" RNAs emerged and replicated to the exclusion of the intended RNAs. Members of this class of selfish molecules, termed RNA Z, amplify efficiently despite their inability to catalyze the target chemical reaction. Their amplification requires the action of both reverse transcriptase and RNA polymerase and involves the synthesis of both DNA and RNA replication intermediates. The proposed amplification mechanism for RNA Z involves the formation of a DNA hairpin that functions as a template for transcription by RNA polymerase. This arrangement links the two strands of the DNA, resulting in the production of RNA transcripts that contain an embedded RNA polymerase promoter sequence.

Non-NASA Center↗

NASA NTRS2009

SiC Optically Modulated Field-Effect Transistor

An optically modulated field-effect transistor (OFET) based on a silicon carbide junction field-effect transistor (JFET) is under study as, potentially, a prototype of devices that could be useful for detecting ultraviolet light. The SiC OFET is an experimental device that is one of several devices, including commercial and experimental photodiodes, that were initially evaluated as detectors of ultraviolet light from combustion and that could be incorporated into SiC integrated circuits to be designed to function as combustion sensors. The ultraviolet-detection sensitivity of the photodiodes was found to be less than desired, such that it would be necessary to process their outputs using high-gain amplification circuitry. On the other hand, in principle, the function of the OFET could be characterized as a combination of detection and amplification. In effect, its sensitivity could be considerably greater than that of a photodiode, such that the need for amplification external to the photodetector could be reduced or eliminated. The experimental SiC OFET was made by processes similar to JFET-fabrication processes developed at Glenn Research Center. The gate of the OFET is very long, wide, and thin, relative to the gates of typical prior SiC JFETs. Unlike in prior SiC FETs, the gate is almost completely transparent to near-ultraviolet and visible light. More specifically: The OFET includes a p+ gate layer less than 1/4 m thick, through which photons can be transported efficiently to the p+/p body interface. The gate is relatively long and wide (about 0.5 by 0.5 mm), such that holes generated at the body interface form a depletion layer that modulates the conductivity of the channel between the drain and the source. The exact physical mechanism of modulation of conductivity is a subject of continuing research. It is known that injection of minority charge carriers (in this case, holes) at the interface exerts a strong effect on the channel, resulting in amplification of the photon-detection signal. A family of operating curves characterizing the OFET can be generated in a series of measurements performed at different intensities of incident ultraviolet light.

Tabib-Azar, Massood↗

NASA NTRS2017

An Intercomparison of Changes Associated with Earth's Lower Tropospheric Temperature Using Traditional and AMIP-Style Reanalyses

Reanalyses have become an integral tool for evaluating regional and global climate variations, and an important component of this is modifications to the energy budget. Reductions in Arctic Sea ice extent has induced an albedo feedback, causing the Arctic to warm more rapidly than anywhere else in the world, referred to as "Arctic Amplification." This has been demonstrated by observations and numerous reanalyses, including the Modern Era Retrospective Analysis for Research and Applications, Version 2 (MERRA-2). However, the Arctic Amplification signal is non-existent in a ten member ensemble of the MERRA-2 Atmospheric Model Intercomparison Project (M2AMIP) simulations, using the same prescribed climate forcing, including Sea Surface Temperature (SST) and ice. An evaluation of the temperature tendency within the lower troposphere due to radiation, moisture, and dynamics as well as the surface energy budget in MERRA-2 and M2AMIP will demonstrate that despite identical prescribed SSTs and sea ice in both versions, enhanced warming in the Arctic in MERRA-2 is in response to the analysis increment tendency due to temperature observations. Furthermore, the role of boundary conditions, model biases and changes in observing systems on the Arctic Amplification signal will be assessed. Literature on the topic of Arctic Amplification demonstrates that the enhanced warming begins in the mid-1990s. Anomalously warm Arctic SST in the early time period of MERRA-2 can mute the trend in Arctic lower troposphere temperature without the constraint of observations in M2AMIP. Additionally, MERRA-2 uses three distinct datasets of SST and sea ice concentration, which also plays a role.

Arcti↗

NASA NTRS2019

Nonmodal Growth of TravelingWaves on Blunt Cones at Hypersonic Speeds

The existing database of transition measurements in hypersonic ground facilities has established that, as the nosetip bluntness is increased, the onset of boundary layer transition over a circular cone at zero angle of attack shifts downstream. However, this trend is reversed at sufficiently large values of the nose Reynolds number, so that the transition onset location eventually moves upstream with a further increase in nose-tip bluntness. Because modal amplification is too weak to initiate transition at moderate-to-large bluntness values, nonmodal growth has been investigated as the potential basis for a physics-based model for the frustum transition. The present analysis investigates the nonmodal growth of traveling disturbances initiated within the nose-tip vicinity that peak within the entropy layer. Results show that, with increasing nose bluntness, both planar and oblique traveling disturbances experience appreciable energy amplification up to successively higher frequencies. For moderately blunt cones, the initial nonmmodal growth is followed by a partial decay that is more than overcome by an eventual, modal growth as Mack-mode waves. For larger bluntness values, the Mack-mode waves are not amplified anywhere upstream of the experimentally measured transition location, but the traveling modes still undergo a significant amount of nonmodal growth. This finding does not provide a definitive link between optimal growth and the onset of transition, but it is qualitatively consistent with the experimental observations that frustum transition in the absence of sufficient Mack-mode amplification implies a double peak in disturbance amplification and the appearance of transitional events above the boundary-layer edge.

Hypersonic speed↗