Search NASA⌕ Search

SEARCH · Search NASA

Results for “Community detection”

Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 235 records · Page 13
All sourcesNASA NTRSDOE OSTI
NASA NTRS1973

Application of ecological, geological and oceanographic ERTS-1 imagery to Delaware's coastal resources planning

The author has identified the following significant results. Coastal vegetation species appearing in the ERTS-1 images taken of Delaware Bay have been correlated with ground truth vegetation maps and imagery obtained from high altitude overflights. Multispectral analysis of the high altitude photographs indicated that four major vegetation communities could be clearly discriminated from 60,000 feet altitude including: (1) salt marsh cord grass; (2) salt marsh hay and spike grass; (3) reed grass; and (4) high tide bush and sea myrtle. In addition, human impact can be detected in the form of fresh water impoundments built to attract water fowl, dredge-fill operations and other alterations of the coastal environment. Overlay maps matching the USGS topographic map size of 1:24,000 have been prepared showing the four wetland vegetation communities, fresh water impoundments, and alteration of wetlands by mosquito control ditching and dredge-fill operations. Using these maps, ERTS-1 images were examined by human interpreters and automated multispectral analyzers. Major plant communities of (1) Spartina alterniflora, (2) Spartina patens and Distichlis spicata, and (3) Iva frutescens and Baccharis halimifolia can be distinguished from each other and from surrounding uplands in ERTS-1 scanner bands 6 and 7.

Klemas, V.↗
NASA NTRSDate not supplied

Exploring the Potential of Hydrogen as a Lift Gas for Nasa’s Scientific Balloon Program

The United States National Aeronautics and Space Administration (NASA) Balloon Program has a rich history of conducting scientific research in the stratosphere, offering a unique platform for experiments in the near-space environment. One critical component of these high-altitude balloons is the choice of lift gas, which is pivotal to achieving the desired altitude and, thus, mission objectives. Traditionally, helium has been the preferred lift gas due to its inert properties and safety considerations. However, the increasing demand and limited global helium reserves have prompted NASA to explore alternative lift gases, with hydrogen emerging as the most logical choice. Hydrogen boasts several advantages, including its abundant availability, cost-effectiveness, and enhanced lift capability compared to helium. Moreover, it can support a wide range of scientific payloads, enabling extended mission durations and increased payload capacities, ultimately strengthening the program’s scientific potential. However, adopting hydrogen as a lift gas is not without challenges. Safety concerns, especially during the launch and recovery phases, are paramount. NASA and the other major players in the scientific ballooning community have been developing stringent safety protocols and engineering solutions to mitigate these risks. This development includes specialized support systems, leak detection mechanisms, and enhanced launch and recovery procedures. Additionally, environmental considerations must be carefully evaluated. Hydrogen release has the potential to increase concentrations of various greenhouse gases, and its usage must be balanced against the benefits it offers to scientific research and exploration. This paper delves into the ongoing research and development efforts to ensure balloon-based research’s long-term sustainability and its ability to continue pushing the boundaries of scientific discovery. This research demonstrates NASA’s commitment to innovation, safety, and environmental responsibility while maintaining its mission to advance our understanding of the Earth and the cosmos.

balloon↗
NASA NTRSDate not supplied

The NASA Disasters Response Coordination System’s (DRCS) Response to the 2024 Hurricane Season

The National Aeronautics and Space Administration (NASA) Disasters Response Coordination System (DRCS) leverages the best available science and expertise to aid federal, state, local, and non-governmental organization (NGO) partners in addressing identified needs during a disaster response. During the 2024 hurricane season, the DRCS activated for seven hurricanes/tropical storms, providing openly available geospatial data through NASA’s Disasters Mapping Portal. Hurricanes Helene and Milton were major hurricanes that impacted the southeastern United States within weeks of one another. Impacts from Helene and Milton to the region included inland flooding, record coastal storm surge, over a thousand landslides, and regional power and telecommunications outages. In response to Helene and Milton, DRCS provided actionable information and products to stakeholders, including Synthetic Aperture Radar (SAR) analysis for landslide and flood detection, Black Marble nighttime lights products for assessing power outages, and Normalized Difference Vegetation Index (NDVI) analysis for post-event vegetation change detection. NASA deployed an Uninhibited Aerial Vehicle SAR (UAVSAR) instrument to collect data on flood extent, providing crucial information on affected communities. Additionally, astronauts aboard the International Space Station (ISS) collected hand-held photography along the paths of Hurricane Helene and Milton to aid in response efforts. Here, we summarize the DRCS responses to Helene and Milton, in particular highlighting the information and products that were provided by the DRCS and how they were utilized by partners to address immediate response needs.

Earth observations↗
NASA NTRS1988

Detecting air pollution stress in southern California vegetation using Landsat Thematic Mapper band data

Landsat Thematic Mapper (TM) and aircraft-borne Thematic Mapper simulator (TMS) data were collected over two areas of natural vegetation in southern California exposed to gradients of pollutant dose, particularly in photochemical oxidants: the coastal sage scrub of the Santa Monica Mountains in the Los Angeles basin, and the yellow pine forests in the southern Sierra Nevada. In both situations, natural variations in canopy closure, with subsequent exposure of understory elements (e.g.,rock or soil, chaparral, grasses, and herbs), were sufficient to cause changes in spectral variation that could obscure differences due to visible foliar injury symptoms observed in the field. TM or TMS data are therefore more likely to be successful in distinguishing pollution injury from background variation when homogeneous communities with closed canopies are subjected to more severe pollution-induced structural and/or compositional change. The present study helps to define the threshold level of vegetative injury detectable by TM data.

Westman, Walter E.↗
DOE OSTI2025

Where do the fish go in winter? A year of observing seasonal changes in Sequim Bay’s nearshore fish community

The use of environmental DNA (eDNA) sampling has been proposed as a complementary method to monitor fish species in marine environments. eDNA offers a non-invasive, cost-effective, and scalable way to detect aquatic species. It is appealing in environments where traditional methods are limited by access or visibility, especially in complex or sensitive habitats such as tidal channels and other marine energy sites. Before eDNA can be fully relied upon, we must verify its accuracy against established methods, like underwater photography. In this study, we collected eDNA samples and concurrently deployed a 360-degree camera near the floating dock of PNNL-Sequim in the tidal channel of Sequim Bay once a month for twelve consecutive months. During deployments, the camera remained on the seafloor for several hours overlapping slack tide and captured time-lapse photographs at ten second intervals. Counts and identifications of fish species observed in the images were used to calculate monthly Shannon diversity and Pielou evenness indices. These values were compared across the months using a Kruskal-Wallis test paired with a Conover-Iman post-hoc test. Cliff’s Delta was also calculated to quantify the effect size of the monthly differences. No fish were observed from December through April, likely due to seasonal behavior changes within the local fish community. Fish returned to the shoreline in May, with the greatest diversity and evenness recorded in September. These findings reveal substantial seasonal variation in nearshore fish communities. Many monthly comparisons were found to contain statistically significant differences within the diversity and evenness, and even more were found to have large effect sizes, signifying large ecological changes throughout the seasons. The absence of fish observed during the winter months is a key outcome of this survey that will hopefully be reflected in the eDNA results still to come, which would help validate the eDNA approach.

59 BASIC BIOLOGICAL SCIENCES↗
NASA NTRS1983

Applications of aerospace technology in biology and medicine

Utilization of NASA technology and its application to medicine is discussed. The introduction of new or improved commercially available medical products and incorporation of aerospace technology is outlined. A biopolar donor-recipient model of medical technology transfer is presented to provide a basis for the methodology. The methodology is designed to: (1) identify medical problems and NASA technology that, in combination, constitute opportunities for successful medical products; (2) obtain the early participation of industry in the transfer process; and (3) obtain acceptance by the medical community of new medical products based on NASA technology. Two commercial transfers were completed: the ocular screening device, a system for quick detection of vision problems in preschool children, and Porta-Fib III, a hospital monitoring unit. Two institutional transfers were completed: implant materials testing, the application of NASA fracture control technology to improve reliability of metallic prostheses, and incinerator monitoring, a quadrupole mass spectrometer to monitor combustion products of municipal incinerators. Mobility aids for the blind and ultrasound diagnosis of burn depth are also studied.

Rouse, D. J.↗
NASA NTRS2006

Antarctic Iceberg Tracking Based on Time Series of Aqua AMSRE Microwave Brightness Temperature Measurements

Observations of icebergs are identified as one of the requirements for the GEOSS (Global Earth Observation System of Systems) in the area of reducing loss of life and property from natural and human-induced disasters. However, iceberg observations are not included among targets in the GEOSS 10-Year Implementation Plan, and thus there is an unfulfilled need for iceberg detection and tracking in the near future. Large Antarctic icebergs have been tracked by the National Ice Center and by the academic community using a variety of satellite sensors including both passive and active microwave imagers, such as SSM/I (Special Sensor Microwave/Imager) deployed on the DMSP (Defense Meteorological Satellite Program) spacecraft. Improvements provided in recent years by NASA and non-NASA satellite radars, scatterometers, and radiometers resulted in an increased number of observed icebergs and even prompted a question: Is The Number of Antarctic Icebergs Really Increasing? [D.G. Long, J. Ballantyne, and C. Bertoia, Eos, Transactions of the American Geophysical Union 83 (42): 469 & 474, 15 October 2002]. AMSR-E (Advanced Microwave Scanning Radiometer for the Earth Observing System) represents an improvement over SSM/I, its predecessor. AMSR-E has more measurement channels and higher spatial resolution than SSM/I. For example, the instantaneous field of view of the AMSR-E s 89-GHz channels is 6 km by 4 km versus 16 km by 14 km for SSM/I s comparable 85-GHz channels. AMSR-E, deployed on the Aqua satellite, scans across a 1450-km swath and provides brightness temperature measurements with nearglobal coverage every one or two days. In polar regions, overlapping swaths generate coverage up to multiple times per day and allow for creation of image time series with high temporal resolution. Despite these advantages, only incidental usage of AMSR-E data for iceberg tracking has been reported so far, none in an operational environment. Therefore, an experiment was undertaken in the RPC (Rapid Prototyping Capability) of NASA s (National Aeronautics and Space Administration) Applied Science Program to demonstrate that passive microwave brightness temperature measurements acquired by AMSR-E can be effectively used to track iceberg movement around Antarctica. The RPC s robust computation environment enabled processing of terabytes of data products available from the NASA Distributed Active Archive Center at the National Snow and Ice Data Center. Iceberg tracking based on the AMSR-E Level 2A data product was compared with records from the National Ice Center for currently existing icebergs. Some icebergs as small as roughly 10 km in size were easily observed, but tracking of many others, even larger ones, was obscured by presence of sea ice surrounding the icebergs. The best results, such as for the large iceberg A22A, were achieved when an iceberg was in open ocean.

Blonski, Slawomir↗
NASA NTRS2019

A Persistent, Large-Scale, and Ordered Electrodynamic Connection Between Saturn and Its Main Rings

Auroral hiss emissions are ubiquitous in planetary magnetospheres, particularly in regions where electric current systems are present. They are generally diagnostic of electrodynamic coupling between conductive bodies, thus making auroral and moon-connected magnetic field lines prime locations for their detection. However, the role of Saturn's rings as a dynamic conductive body has been elusive and of great interest to the community. Cassini's Grand Finale orbits afforded a unique opportunity to directly sample magnetic field lines connected to the main rings. Here we provide strong evidence for the persistent and organized presence of auroral hiss demonstrably associated with the main rings. This is in contrast to recent observations suggesting that Saturn's rings may be barriers to field-aligned currents. Our results provide a new view of Saturn's rings as a dynamic system that is in continuous and ordered electrodynamic coupling with the planet.

Sulaiman, A. H.↗
NASA NTRS2000

Passive, Direct-Read Monitoring System for Selective Detection and Quantification of Hydrogen Chloride

Monitoring the exposure of an employee to hydrogen chloride or hydrochloric acid in the presence of other acids has been a challenge to the industrial hygiene community. The capability of a device to differentiate the levels of acid vapors would allow for more accurate determinations of exposure and therefore improved occupational health. In this work, a selective direct-read colorimetric badge system was validated for Short Term Exposure Limit (STEL) monitoring of hydrogen chloride. The passive colorimetric badge system consists of a direct reading badge and a color scale. The badge has a coated indicator layer with a diffusive resistance in the shape of an exclamation mark. An exclamation mark will appear if hydrogen chloride is present in the atmosphere at concentrations at or above 2.0 ppm. By using the color scale, the intensity of the color formed on the badge can be further quantified up to 25 ppm. The system was validated according to a protocol based on the NIOSH Protocol for the Evaluation of Passive Monitors. The badge was exposed to relative humidities ranging from 11% to 92%, temperatures ranging from 7 C to 400 C and air velocities ranging from 5 cm/sec to 170 cm/sec. All experiments were conducted in a laboratory vapor generation system. Hydrofluoric acid, nitric acid, sulfuric acid, chlorine, hydrogen sulfide and organic acids showed no effect on the performance of the hydrogen chloride monitoring system. The passive badge and color scale system exceeded the accuracy requirements as defined by NIOSH. At ambient conditions, the mean coefficient of variation was 10.86 and the mean bias was 1.3%. This data was presented previously at the American Industrial Hygiene Conference and Exposition in Toronto, Canada in June 1999.

Chapman, K. B.↗
NASA NTRS1992

Novel long-chain anteiso-alkanes and anteiso-alkanoic acids in Antarctic rocks colonized by living and fossil cryptoendolithic microorganisms

Saponified extracts of rock samples colonized by cryptoendolithic microbial communities from the McMurdo Dry Valleys of Southern Victoria Land, Antarctica, were separated into hydrocarbon and fatty acid fractions by silica gel column chromatography. Hydrocarbons and methyl esters of fatty acids were analyzed by capillary gas chromatography-mass spectrometry. Unusually, a suite of long-chain anteiso-alkanes (a-C20 to a-C30) and anteiso-alkanoic acids (a-C20 to a-C30) were detected in many samples, together with straight-chain, branched and/or cyclic and acyclic isoprenoid compounds. These novel compounds are probably derived from unidentified heterotrophic bacteria or symbiotic processes in a unique microbial community in the Antarctic cold desert and suggest the occurrence of a special biosynthetic pathway. Long-chain anteiso-alkanes are probably formed through microbial decarboxylation of corresponding anteiso-alkanoic acids. They may serve as new biomarkers in environmental and geochemical studies.

NASA Program Exobiology↗
NASA NTRS2022

Un-Numbered Operational Areas PRL 229 Railroad Tie Disposal Area Pond Confirmation Sampling Report

This Confirmatory Sampling (CS) Report (CSR) was prepared for the National Aeronautics and Space Administration (NASA), Kennedy Space Center (KSC), Florida in accordance with the CS Work Plan (CSWP) (HGL, 2022a). The CSR summarizes investigation activities performed on the Railroad Tie Disposal Area (RTDA) of the Un-Numbered Operational Areas (UNOA), Potential Release Location (PRL) 229 located at KSC. HydroGeoLogic, Inc. (HGL) prepared this report under Contract Number 80KSC019D0012. The purpose of the CSR is to provide information to evaluate the level of potential contamination in surface water and sediment samples collected from the RTDA PRL 229 Pond and determine if contaminants pose unacceptable risks to ecological receptors. The CSR provides analytical data of 10 sets of surface water and sediment samples collected in 2021. Surface water samples were analyzed for volatile organic compounds (VOCs), semi-volatile organic compounds (SVOCs), polynuclear aromatic hydrocarbon (PAHs), metals, hardness, and salinity, while sediment samples were analyzed for metals, total organic carbon (TOC), SVOCs, PAHs, and total petroleum hydrocarbons (TPH). The CSR includes a Screening Level Ecological Risk Assessment (SLERA) conducted under KSC’s Resource Conservation and Recovery Act (RCRA) Corrective Action Program to determine if unacceptable risks to ecological receptors exist at PRL 229 Pond. Metals and PAHs were detected in surface water samples, including detections of antimony, arsenic, barium, calcium, copper, lead, magnesium, thallium, zinc, anthracene, benzo(a)anthracene, benzo(a)pyrene, benzo(b)fluoranthene, benzo(g,h,i)perylene, benzo(k)fluoranthene, chrysene, dibenzo(a,h)anthracene, fluoranthene, indeno(1,2,3-c,d)pyrene and pyrene. Based on measured salinity (1.03 to 1.21 parts per thousand), the pond was identified as freshwater. Sediment analytical results also contained detections of metals and PAHs, including antimony, arsenic, barium, beryllium, cadmium, chromium, copper, lead, mercury, nickel, selenium, thallium, zinc, anthracene benzo(a)anthracene, benzo(a)pyrene, benzo(b)fluoranthene, benzo(g,h,i)perylene, benzo(k)fluoranthene, chrysene, dibenzo(a,h)anthracene, fluoranthene, indeno(1,2,3-c,d)pyrene and pyrene. Potential risks to ecological receptors were evaluated in accordance with the SLERA Work Plan and the Decision Process Document for the Resource Conservation and Recovery Act (RCRA) Corrective Action Program at KSC (DPD) (Geosyntec, 2019), and using the 2021 sediment and surface water analytical results. Potential ecological receptors evaluated in the SLERA include the aquatic community, benthic invertebrates, aquatic birds, and aquatic mammals. The SLERA included Steps 2a through 2c of the Ecological Risk Assessment (ERA) process in accordance with the DPD. Using multiple lines of evidence, the SLERA concluded that many of the detected analytes are present at concentrations similar to those in background or reference locations at KSC, and/or at concentrations that would not pose a significant risk to ecological communities. There are no screening values available for the avian community, and PAHs are bioaccumulative compounds. For this reason, food web modeling was completed as a supplement to Step 2c to provide a quantitative evaluation of this exposure pathway. Food web modeling indicates that PAHs are unlikely to pose a risk to birds exposed to site sediment and surface water. Based on the results of the SLERA, the contaminants in sediment and surface water pose no to minimal risk to the ecological communities that could live or forage at the site. Based on the results of the SLERA, no further study is recommended for the RTDA Pond at PRL 229.

Confirmation Sampling↗
DOE OSTI2025

Three‐trophic level food webs support the safety of a biocontrol agent 3 years after release

Biological control (biocontrol) is a powerful tool for managing invasive alien species and assisting the restoration of native ecosystems. Rigorous post‐release monitoring of biocontrol agents is critical to evaluate the success of biocontrol programs; however, this is still rarely implemented. Here, we combined the use of species interaction networks with a Before‐After Control‐Impact design to evaluate the target and non‐target, direct and indirect effects of the Australian gall wasp Trichilogaster acaciaelongifoliae , released to control the invasive plant Acacia longifolia in Portugal. We compared the structure of plant‐galling insect‐parasitoid food webs before and 3 years after the release of the biocontrol agent. Exhaustive sampling did not detect any non‐target effects, either direct (on non‐target plants) or indirect (on other galling insects via shared plants). Additionally, no significant changes were detected in network structure that could be related to the establishment of the biocontrol agent. This study shows that monitoring biocontrol at the community level is possible and that, when carefully planned, biocontrol poses minimal risk of non‐target effects.

López‐Núñez, Francisco A. [Centre for Functional E↗
NASA NTRS2003

Light Detection and Ranging-Based Terrain Navigation: A Concept Exploration

This paper discusses the use of Airborne Light Detection And Ranging (LiDAR) equipment for terrain navigation. Airborne LiDAR is a relatively new technology used primarily by the geo-spatial mapping community to produce highly accurate and dense terrain elevation maps. In this paper, the term LiDAR refers to a scanning laser ranger rigidly mounted to an aircraft, as opposed to an integrated sensor system that consists of a scanning laser ranger integrated with Global Positioning System (GPS) and Inertial Measurement Unit (IMU) data. Data from the laser range scanner and IMU will be integrated with a terrain database to estimate the aircraft position and data from the laser range scanner will be integrated with GPS to estimate the aircraft attitude. LiDAR data was collected using NASA Dryden's DC-8 flying laboratory in Reno, NV and was used to test the proposed terrain navigation system. The results of LiDAR-based terrain navigation shown in this paper indicate that airborne LiDAR is a viable technology enabler for fully autonomous aircraft navigation. The navigation performance is highly dependent on the quality of the terrain databases used for positioning and therefore high-resolution (2 m post-spacing) data was used as the terrain reference.

Campbell, Jacob↗
NASA NTRS2010

Laser Interferometry for Gravitational Wave Observation: LISA and LISA Pathfinder

The Laser Interferometer Space Antenna (LISA) is a planned NASA-ESA gravitational wave observatory in the frequency range of 0.1mHz-100mHz. This observation band is inaccessible to ground-based detectors due to the large ground motions of the Earth. Gravitational wave sources for LISA include galactic binaries, mergers of supermasive black-hole binaries, extreme-mass-ratio inspirals, and possibly from as yet unimagined sources. LISA is a constellation of three spacecraft separated by 5 million km in an equilateral triangle, whose center follows the Earth in a heliocentric orbit with an orbital phase offset oF 20 degrees. Challenging technology is required to ensure pure geodetic trajectories of the six onboard test masses, whose distance fluctuations will be measured by interspacecraft laser interferometers with picometer accuracy. LISA Pathfinder is an ESA-launched technology demonstration mission of key LISA subsystems such us spacecraft control with micro-newton thrusters, test mass drag-free control, and precision laser interferometry between free-flying test masses. Ground testing of flight hardware of the Gravitational Reference Sensor and Optical Metrology subsystems of LISA Pathfinder is currently ongoing. An introduction to laser interferometric gravitational wave detection, ground-based observatories, and a detailed description of the two missions together with an overview of current investigations conducted by the community will bc discussed. The current status in development and implementation of LISA Pathfinder pre-flight systems and latest results of the ongoing ground testing efforts will also be presented

Guzman, Felipe↗
NASA NTRS2014

A Simplified Approach to Cloud Masking with VIIRS in the S-NPP/JPSS Era

The quantitative detection of clouds in satellite imagery has a number of important applications in weather analysis. The proper interpretation of satellite imagery for improved situational awareness depends on knowing where the clouds are at all times of the day. Additionally, many products derived from infrared measurements need accurate cloud information to mask out regions where retrieval of geophysical parameters in the atmosphere or on the surface are not possible. Thus, the accurate detection of the presence of clouds in satellite imagery on a global basis is important to the product developers and the operational weather community to support their decision‐making process. This abstract describes an application of a two‐channel bispectral composite threshold (BCT) approach applied to VIIRS imagery. The simplified BCT approach uses only the 10.76 and 3.75 micrometer spectral channels in two spectral tests; a straightforward infrared threshold test with the longwave channel and a shortwave minus longwave channel difference test. The key to the success of this approach as demonstrated in past applications to GOES and MODIS data is the generation of temporally and spatially dependent thresholds used in the tests from a previous number of days at similar observations to the current data. The presentation will present an overview of the approach and intercomparison results with other satellites, methods, and against verification data.

Jedlovec, Gary J.↗
NASA NTRSDate not supplied

Graph Convolutional Network-Strengthened Topic Modeling for Scientific Papers

Machine learning has been woven into statistics to modernize topic modeling over textual documents written in natural language, and scientific paper search and recommendation can consequently offer higher accuracy instead of counting on traditional keyword-based search. However, topic distribution of a paper resulted from existing topic modeling techniques only relies on the statistics of words contained in the paper itself. We argue that community users’ views of a paper may also provide insights at the time of recommendation. For example, if a paper on fake image detection has been cited heavily by machine learning papers, such a feature should be absorbed in the embedding of this paper, so that it can be recommended for future query on machine learning. In this paper, we present a Graph Convolutional Network-strengthened Topic Modeling (GCN-TM) method, which employs GCN technique to refine topic modeling of scientific papers. A citation-oriented knowledge graph is constructed, and topic modeling is mapped to feature embedding of the comprising papers. On top of its own topics carried in its content, each paper learns topics from its neighbors and revise its embedding accordingly. Our empirical studies over real-life scientific literature has proved the necessity and effectiveness of our proposed approach.

Jia Zhang↗
NASA NTRS2001

Ectomycorrhizal fungal associates of Pinus contorta in soils associated with a hot spring in Norris Geyser Basin, Yellowstone National Park, Wyoming

Molecular methods and comparisons of fruiting patterns (i.e., presence or absence of fungal fruiting bodies in different soil types) were used to determine ectomycorrhizal (EM) associates of Pinus contorta in soils associated with a thermal soil classified as ultra-acidic to extremely acidic (pH 2 to 4). EM were sampled by obtaining 36 soil cores from six paired plots (three cores each) of both thermal soils and forest soils directly adjacent to the thermal area. Fruiting bodies (mushrooms) were collected for molecular identification and to compare fruiting body (above-ground) diversity to below-ground diversity. Our results indicate (i) that there were significant decreases in both the level of EM infection (130 +/- 22 EM root tips/core in forest soil; 68 +/- 22 EM root tips/core in thermal soil) and EM fungal species richness (4.0 +/- 0.5 species/core in forest soil; 1.2 +/- 0.2 species/core in thermal soil) in soils associated with the thermal feature; (ii) that the EM mycota of thermal soils was comprised of a small set of dominant species and included very few rare species, while the EM mycota of forest soils contained a few dominant species and several rare EM fungal species; (iii) that Dermocybe phoenecius and a species of Inocybe, which was rare in forest soils, were the dominant EM fungal species in thermal soils; (iv) that other than the single Inocybe species, there was no overlap in the EM fungal communities of the forest and thermal soils; and (v) that the fungal species forming the majority of the above-ground fruiting structures in thermal soils (Pisolithus tinctorius, which is commonly used in remediation of acid soils) was not detected on a single EM root tip in either type of soil. Thus, P. tinctorius may have a different role in these thermal soils. Our results suggest that this species may not perform well in remediation of all acid soils and that factors such as pH, soil temperature, and soil chemistry may interact to influence EM fungal community structure. In addition, we identified at least one new species with potential for use in remediation of hot acidic soil.

Pinus/microbiology↗
NASA NTRS1998

Rapid System to Quantitatively Characterize the Airborne Microbial Community

Bioaerosols have been linked to a wide range of different allergies and respiratory illnesses. Currently, microorganism culture is the most commonly used method for exposure assessment. Such culture techniques, however, generally fail to detect between 90-99% of the actual viable biomass. Consequently, an unbiased technique for detecting airborne microorganisms is essential. In this Phase II proposal, a portable air sampling device his been developed for the collection of airborne microbial biomass from indoor (and outdoor) environments. Methods were evaluated for extracting and identifying lipids that provide information on indoor air microbial biomass, and automation of these procedures was investigated. Also, techniques to automate the extraction of DNA were explored.

Macnaughton, Sarah J.↗