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NASA NTRS2010

Psychrophilic and Psychrotolerant Microbial Extremophiles in Polar Environments

The microbial extremophiles that inhabit the polar regions of our planet are of tremendous significance. The psychrophilic and psychrotolerant microorganisms, which inhabit all of the cold environments on Earth have important applications to Bioremediation, Medicine, Pharmaceuticals, and many other areas of Biotechnology. Until recently, most of the research on polar microorganisms was confined to studies of polar diatoms, yeast, fungi and cyanobacteria. However, within the past three decades, extensive studies have been conducted to understand the bacteria and archaea that inhabit the Arctic and Antarctic sea-ice, glaciers, ice sheets, permafrost and the cryptoendolithic, cryoconite and ice-bubble environments. These investigations have resulted in the discovery of many new genera and species of anaerobic and aerobic microbial extremophiles. Exotic enzymes, cold-shock proteins and pigments produced by some of the extremophiles from polar environments have the potential to be of great benefit to Mankind. Knowledge about microbial life in the polar regions is crucial to understanding the limitations and biodiversity of life on Earth and may provide valuable clues to the Origin of Life on Earth. The discovery of viable microorganisms in ancient ice from the Fox Tunnel, Alaska and the deep Vostok Ice has shown that microorganisms can remain alive while cryopreserved in ancient ice. The psychrophilic lithoautotrophic homoacetogen isolated from the deep anoxic trough of Lake Untersee is an ideal candidate for life that might inhabit comets or the polar caps of Mars. The spontaneous release of gas from within the Anuchin Glacier above Lake Untersee may provide clues to the ice geysers that erupt from the tiger stripe regions of Saturn s moon Enceladus. The methane productivity in the lower regimes of Lake Untersee may also provide insights into possible mechanisms for the recently discovered methane releases on Mars. Since most of the other water bearing bodies of our Solar system are frozen worlds, microbial extremophiles from the Polar Regions of Earth are of great importance to Astrobiology in understanding where and how to search for evidence of life elsewhere in the Cosmos.

Hoover, Richard B.↗

NASA NTRS2010

Developing Biological ISRU: Implications for Life Support and Space Exploration

Main findings: 1) supplementing very dilute media for cultivation of CB with analogs of lunar or Martian regolith effectively supported the proliferation of CB; 2) O2 evolution by siderophilic cyanobacteria cultivated in diluted media but supplemented with iron-rich rocks was higher than O2 evolution by same strain in undiluted medium; 3) preliminary data suggest that organic acids produced by CB are involved in iron-rich mineral dissolution; 4) the CB studied can accumulate iron on and in their cells; 4) sequencing of the cyanobacterium JSC-1 genome revealed that this strain possesses molecular features which make it applicable for the cultivation in special photoreactors on Moon and Mars. Conclusion: As a result of pilot studies, we propose, to develop a concept for semi-closed integrated system that uses CB to extract useful elements to revitalize air and produce valuable biomolecules. Such a system could be the foundation of a self-sustaining extraterrestrial outpost (Hendrickx, De Wever et al., 2005; Handford, 2006). A potential advantage of a cyanobacterial photoreactor placed between LSS and ISRU loops is the possibility of supplying these systems with extracted elements and compounds from the regolith. In addition, waste regolith may be transformed into additional products such as methane, biomass, and organic and inorganic soil enrichment for the cultivation of higher plants.

Brown, I. I.↗

NASA NTRS2010

Aviation Fueling: A Cleaner, Greener Approach

Projected growth of aviation depends on fueling where specific needs must be met. Safety is paramount, and along with political, social, environmental and legacy transport systems requirements, alternate aviation fueling becomes an opportunity of enormous proportions. Biofuels sourced from halophytes, algae, cyanobacteria, and weeds using wastelands, waste water, and seawater have the capacity to be drop-in fuel replacements for petroleum fuels. Biojet fuels from such sources solves the aviation CO2 emissions issue and do not compete with food or freshwater needs. They are not detrimental to the social or environmental fabric and use the existing fuels infrastructure. Cost and sustainable supply remains the major impediments to alternate fuels. Halophytes are the near-term solution to biomass/biofuels capacity at reasonable costs; they simply involve more farming, at usual farming costs. Biofuels represent a win-win approach, proffering as they do at least the ones we are studying massive capacity, climate neutral-to-some sequestration, and ultimately, reasonable costs.

Hendricks, Robert C.↗

NASA NTRS2010

Novel Thermotolerant Siderophilic Filamentous Cyanobacterium that Produces Intracellular Iron-Rich Phases

Cyanobacteria are the main producers of organic compounds in iron-depositing hot springs despite photosynthetically generated-oxygen and the abundance of reduced iron (Fe2+) that likely leads to enormous oxidative stress within cyanobacterial cells. Therefore, the study of cyanobacterial diversity, phylogeny, and biogeochemical activity in iron-depositing hot springs will not only provide insights into the contribution of CB to iron redox cycling in these environments, but it could also provide insights into CB evolution. This study characterizes the phylogeny, morphology, and physiology of isolate JSC-1, a novel filamentous CB isolated from an iron-depositing hot spring. While isolate JSC-1 is morphologically similar to the CB genus Leptolyngbya, 16S rDNA sequence data indicated that it shares 95 percent sequence similarity to the type strain L. boryanum. Strain JSC-1 fixes N2 and exhibited an unusually high ratio between photosystem (PS) I and PS II and was capable of complementary chromatic adaptation. Further, it synthesized only chlorophyll a and a unique set of carotenoids. Strain JSC-1 not only required high levels of Fe for growth (greater than or equal to 40 microM), but it also accumulated large amounts of extracellular ferrihydrite and generated intracellular ferric phosphates. Strain JSC-1 was found to secrete 2-oxoglutaric acid and possesses one ortholog and one paralog of bacterioferritin. Surprisingly, the latter has 70.13 % identity with a bacterioferritin in marine-proteobacterium HTCC 2080 and has joint node with bacterioferritins found in enterobacteria. Collectively, these observations provide insights into the physiological strategies that might have allowed CB to develop and proliferate in Fe-rich environments. Based on its genotypic and phenotypic characterization of strain, JSC-1 represents a new operational taxonomical unit (OTU) JSC-1.

Broun, Igor I.↗

NASA NTRS2010

Use of MODIS Terra Imagery to Estimate Surface Water Quality Standards, Using Lake Thonotosassa, Florida, as a Case Study

Lake Thonotosassa is a highly eutrophied lake located in an area with rapidly growing population in the Tampa Bay watershed, Florida. The Florida Administrative Code has designated its use for "recreation, propagation and maintenance of a healthy, well-balanced population of fish and wildlife." Although this lake has been the subject of efforts to improve water quality since 1970, overall water quality has remained below the acceptable state standards, and has a high concentration of nutrients. This condition is of great concern to public health since it has favored episodic blooms of Cyanobacteria. Some Cyanobacterial species release toxins that can reach humans through drinking water, fish consumption, and direct contact with contaminated water. The lake has been historically popular for fishing and water sports, and its overflow water drains into the Hillsborough River, the main supply of municipal water for the City of Tampa, this explains why it has being constantly monitored in situ for water quality by the Environmental Protection Commission of Hillsborough County (EPC). Advances in remote sensing technology, however, open the possibility of facilitating similar types of monitoring in this and similar lakes, further contributing to the implementation of surveillance systems that would benefit not just public health, but also tourism and ecosystems. Although traditional application of this technology to water quality has been focused on much larger coastal water bodies like bays and estuaries, this study evaluates the feasibility of its application on a 46.6 km2 freshwater lake. Using surface reflectance products from Moderate-Resolution Imaging Spectroradiometer (MODIS) Terra, this study evaluates associations between remotely sensed data and in situ data from the EPC. The parameters analyzed are the surface water quality standards used by the State of Florida and general indicators of trophic status.

Moreno, Max J.↗

NASA NTRS2011

Filamentous Trichomic Prokaryotes in Carbonaceous Meteorites: Indigenous Microfossils, Minerals, or Modern Bio-Contaminants?

Large complex filaments have been detected in freshly fractured interior surfaces of a variety of carbonaceous meteorites. Many exhibit the detailed morphological and morphometric characteristics of known filamentous trichomic prokaryotic microorganisms. In this paper we review prior studies of filamentous microstructures encountered in the meteorites along with the elemental compositions and characteristics of the, fibrous evaporite minerals and filamentous cyanobacteria and homologous trichomic sulfur bacteria. The meteorite images and elemental compositions will compared with data obtained with the same instruments for abiotic microstructures and living and fossil microorganisms in order to evaluate the relative merits of the alternate hypotheses that have been advanced to explain the nature and characteristics of the meteorite filaments. The possibiility that the filaments found in the meteorites may be comprise modern bio-contaminants will be evaluated in light of their observed elemental compositions and data by other researchers on the detection of indigenous complex organic biosignatures, and extraterrestrial amino acids and nucleobases found in the Murchison CM2 and the Orgueil CI1 carbonaceous meteorites.

Hoover, Richard B.↗

NASA NTRS2011

Comets as Parent Bodies of CI1 Carbonaceous Meteorites and Possible Habitats of Ice-Microbiota

Recent studies of comets and cometary dust have confirmed the presence of biologically relevant organic molecules along with clay minerals and water ice. It is also now well established by deuterium/hydrogen ratios that the CI1 carbonaceous meteorites contain indigenous extraterrestrial water. The evidence of extensive aqueous alteration of the minerals in these meteorites led to the hypothesis that water-bearing asteroids or comets represent the parent bodies of the CI1 (and perhaps CM2) carbonaceous meteorites. These meteorites have also been shown to possess a diverse array of complex organics and chiral and morphological biomarkers. Stable isotope studies by numerous independent investigators have conclusively established that the complex organics found in these meteorites are both indigenous and extraterrestrial in nature. Although the origin of these organics is still unknown, some researchers have suggested that they originated by unknown abiotic mechanisms and may have played a role in the delivery of chiral biomolecules and the origin of life on Early Earth. In this paper we review these results and investigate the thermal history of comets. We show that permanent as well as transient domains of liquid water can be maintained on a comet under a plausible set of assumptions. With each perihelion passage of a comet volatiles are preferentially released, and during millions of such passages the comet could shed crustal debris that may survive transit through the Earth s atmosphere as a carbonaceous meteorite. We review the current state of knowledge of comets and carbonaceous meteorites. We also present the results of recent studies on the long-term viability of terrestrial ice-microbiota encased in ancient glacial ice and permafrost. We suggest that the conditions which have been observed to prevail on many comets do not preclude either survivability (or even the active metabolism and growth) of many types of eukaryotic and prokaryotic microbial extremophiles-including algae, cyanobacteria, bacteria and archaea. It is argued that the chemical and morphological biomarkers detected on comets and carbonaceous meteorites can be explained by ancient microbial activity without the need to invoke unknown abiotic production mechanisms.

Wickramasinghe, N. Chandra↗

NASA NTRS2012

Rates of Dinitrogen Fixation and the Abundance of Diazotrophs in North American Coastal Waters Between Cape Hatteras and Georges Bank

We coupled dinitrogen (N2) fixation rate estimates with molecular biological methods to determine the activity and abundance of diazotrophs in coastal waters along the temperate North American Mid-Atlantic continental shelf during multiple seasons and cruises. Volumetric rates of N2 fixation were as high as 49.8 nmol N L(sup -1) d(sup -1) and areal rates as high as 837.9 micromol N m(sup -2) d(sup -1) in our study area. Our results suggest that N2 fixation occurs at high rates in coastal shelf waters that were previously thought to be unimportant sites of N2 fixation and so were excluded from calculations of pelagic marine N2 fixation. Unicellular N2-fixing group A cyanobacteria were the most abundant diazotrophs in the Atlantic coastal waters and their abundance was comparable to, or higher than, that measured in oceanic regimes where they were discovered. High rates of N2 fixation and the high abundance of diazotrophs along the North American Mid-Atlantic continental shelf highlight the need to revise marine N budgets to include coastal N2 fixation. Integrating areal rates of N2 fixation over the continental shelf area between Cape Hatteras and Nova Scotia, the estimated N2 fixation in this temperate shelf system is about 0.02 Tmol N yr(sup -1), the amount previously calculated for the entire North Atlantic continental shelf. Additional studies should provide spatially, temporally, and seasonally resolved rate estimates from coastal systems to better constrain N inputs via N2 fixation from the neritic zone.

Middle Atlantic Bight↗

NASA NTRS2014

Salt Tolerance and Polyphyly in the Cyanobacterium Chroococcidiopsis (Pleurocapsales)1

Chroococcidiopsis Geitler (Geitler 1933) is a genus of cyanobacteria containing desiccation and radiation resistant species. Members of the genus live in habitats ranging from hot and cold deserts to fresh and saltwater environments. Morphology and cell division pattern have historically been used to define the genus. To better understand the genetic and phenotypic diversity of the genus, 15 species were selected that had been previously isolated from different locations, including salt and freshwater environments. Four markers were sequenced from these 15 species, the 16S rRNA, rbcL, desC1 and gltX genes. Phylogenetic trees were generated which identified two distinct clades, a salt-tolerant clade and a freshwater clade. This study demonstrates that the genus is polyphyletic based on saltwater and freshwater phenotypes. To understand the resistance to salt in more details, species were grown on a range of sea salt concentrations which demonstrated that the freshwater species were salt-intolerant whilst the saltwater species required salt for growth. This study shows an increased resolution of the phylogeny of Chroococcidiopsis and provides further evidence that the genus is polyphyletic and should be reclassified to improve clarity in the literature.

Genus↗

NASA NTRS2014

Interannual Variation in Phytoplankton Class-Specific Primary Production at a Global Scale

We used the NASA Ocean Biogeochemical Model (NOBM) combined with remote sensing data via assimilation to evaluate the contribution of 4 phytoplankton groups to the total primary production. First we assessed the contribution of each phytoplankton groups to the total primary production at a global scale for the period 1998-2011. Globally, diatoms were the group that contributed the most to the total phytoplankton production (50, the equivalent of 20 PgC y-1. Coccolithophores and chlorophytes each contributed to 20 (7 PgC y-1 of the total primary production and cyanobacteria represented about 10 (4 PgC y(sub-1) of the total primary production. Primary production by diatoms was highest in high latitude (45) and in major upwelling systems (Equatorial Pacific and Benguela system). We then assessed interannual variability of this group-specific primary production over the period 1998-2011. Globally the annual relative contribution of each phytoplankton groups to the total primary production varied by maximum 4 (1-2 PgC y-1. We assessed the effects of climate variability on the class-specific primary production using global (i.e. Multivariate El Nio Index, MEI) and regional climate indices (e.g. Southern Annular Mode (SAM), Pacific Decadal Oscillation (PDO) and North Atlantic Oscillation (NAO)). Most interannual variability occurred in the Equatorial Pacific and was associated with climate variability as indicated by significant correlation (p 0.05) between the MEI and the class-specific primary production from all groups except coccolithophores. In the Atlantic, climate variability as indicated by NAO was significantly correlated to the primary production of 2 out of the 4 groups in the North Central Atlantic (diatomscyanobacteria) and in the North Atlantic (chlorophytes and coccolithophores). We found that climate variability as indicated by SAM had only a limited effect on the class-specific primary production in the Southern Ocean. These results provide a modeling and data assimilation perspective to phytoplankton partitioning of primary production and contribute to our understanding of the dynamics of the carbon cycle in the oceans at a global scale.

Chl-a↗

NASA NTRS2013

Interannual Variation in Phytoplankton Primary Production at a Global Scale

We used the NASA Ocean Biogeochemical Model (NOBM) combined with remote sensing data via assimilation to evaluate the contribution of four phytoplankton groups to the total primary production. First, we assessed the contribution of each phytoplankton groups to the total primary production at a global scale for the period 1998-2011. Globally, diatoms contributed the most to the total phytoplankton production ((is)approximately 50%, the equivalent of 20 PgC·y1). Coccolithophores and chlorophytes each contributed approximately 20% ((is) approximately 7 PgC·y1) of the total primary production and cyanobacteria represented about 10% ((is) approximately 4 PgC·y1) of the total primary production. Primary production by diatoms was highest in the high latitudes ((is) greater than 40 deg) and in major upwelling systems (Equatorial Pacific and Benguela system). We then assessed interannual variability of this group-specific primary production over the period 1998-2011. Globally the annual relative contribution of each phytoplankton groups to the total primary production varied by maximum 4% (1-2 PgC·y1). We assessed the effects of climate variability on group-specific primary production using global (i.e., Multivariate El Niño Index, MEI) and "regional" climate indices (e.g., Southern Annular Mode (SAM), Pacific Decadal Oscillation (PDO) and North Atlantic Oscillation (NAO)). Most interannual variability occurred in the Equatorial Pacific and was associated with climate variability as indicated by significant correlation (p (is) less than 0.05) between the MEI and the group-specific primary production from all groups except coccolithophores. In the Atlantic, climate variability as indicated by NAO was significantly correlated to the primary production of 2 out of the 4 groups in the North Central Atlantic (diatoms/cyanobacteria) and in the North Atlantic (chlorophytes and coccolithophores). We found that climate variability as indicated by SAM had only a limited effect on group-specific primary production in the Southern Ocean. These results provide a modeling and data assimilation perspective to phytoplankton partitioning of primary production and contribute to our understanding of the dynamics of the carbon cycle in the oceans at a global scale.

Coccolithophores↗

NASA NTRS2015

NASA's International Space Station: A Testbed for Planetary Protection Protocol Development

Wherever humans go, they inevitably carry along the critters that live in and on them. Conventional wisdom has long held that it is unlikely those critters could survive the space environment, but in 2007 some microscopic aquatic animals called Tardigrades survived exposure to space and in 2008 Cyanobacteria lived for 548 days outside the ISS. Unlike the Mars rovers that were cleaned once and sent on their way, crew members will provide a constantly regenerating contaminant source. Are we prepared to certify that we can meet forward contamination protocols as we search for life at new destinations? What about the organisms we might reasonably expect a crewed spacecraft to leak or vent? Do we even know what they are? How long might our tiny hitch-hikers survive in close proximity to a warm spacecraft that periodically leaks/vents water or oxygen and how might they mutate with long-duration exposure? How will these contaminants migrate from their source in conditions encountered in space or on other planetary surfaces? This project aims to answer some of these questions by bringing together key stakeholder communities to develop a human forward contamination test, analysis, and integration plan. A system engineering approach to identify the experiments, analysis, and modeling needed to develop the contamination control protocols required will be used as a roadmap to integrate the many different parts of this problem - from launch to landing, living, and working on another planetary surface.

Bell, M. S.↗

NASA NTRS2015

Nasa's International Space Station: A Testbed for Planetary Protection Protocol Development

Bell, M. S.↗

NASA NTRS2015

Characterize Human Forward Contamination Project

Let's face it: wherever we go, we will inevitably carry along the little critters that live in and on us. Conventional wisdom has long held that it's unlikely those critters could survive the space environment, but in 2007 microscopic animals called Tardigrades survived exposure to space and in 2008 Cyanobacteria lived for 548 days outside the International Space Station (ISS). But what about the organisms we might reasonably expect a crewed spacecraft to leak or vent? Do we even know what they are? How long might our tiny hitch-hikers survive in close proximity to a warm spacecraft that periodically leaks/vents water or oxygen-and how might they mutate with long-duration exposure? Unlike the Mars rovers that we cleaned once and sent on their way, crew members will provide a constantly regenerating contaminant source. Are we prepared to certify that we can meet forward contamination protocols as we search for life at new destinations? This project has four technical objectives: 1. TEST: Develop a test plan to leverage existing equipment (i.e. ISS) to characterize the kinds of organisms we can reasonably expect pressurized, crewed volumes to vent or leak overboard; 2. ANALYSIS: Develop an analysis plan to study those organisms in relevant destination environments, including spacecraft-induced conditions; 3. MODEL: Develop a modeling plan to model organism transport mechanisms in relevant destination environments; 4. SHARE: Develop a plan to disseminate findings and integrate recommendations into exploration requirements & ops. In short, we propose a system engineering approach to roadmap the necessary experiments, analysis, and modeling up front--rather than try to knit together disparate chunks of data into a sensible conclusion after the fact.

Rucker, Michelle↗

NASA NTRS2015

Characterize Human Forward Contamination Project

Let's face it: wherever we go, we will inevitably carry along the little critters that live in and on us. Conventional wisdom has long held that it's unlikely those critters could survive the space environment, but in 2007 microscopic animals called Tardigrades survived exposure to space and in 2008 Cyanobacteria lived for 548 days outside the International Space Station (ISS). But what about the organisms we might reasonably expect a crewed spacecraft to leak or vent? Do we even know what they are? How long might our tiny hitch-hikers survive in close proximity to a warm spacecraft that periodically leaks/vents water or oxygen-and how might they mutate with long-duration exposure? Unlike the Mars rovers that we cleaned once and sent on their way, crew members will provide a constantly regenerating contaminant source. Are we prepared to certify that we can meet forward contamination protocols as we search for life at new destinations? This project has four technical objectives: 1. TEST: Develop a test plan to leverage existing equipment (i.e. ISS) to characterize the kinds of organisms we can reasonably expect pressurized, crewed volumes to vent or leak overboard; as part of testing, we'll need to develop an Extravehicular Activity (EVA)-compatible tool that can withstand the pressure and temperature extremes of space, as well as collect, separate, and store multiple samples; 2. ANALYSIS: Develop an analysis plan to study those organisms in relevant destination environments, including spacecraft-induced conditions; 3. MODEL: Develop a modeling plan to model organism transport mechanisms in relevant destination environments; 4. SHARE: Develop a plan to disseminate findings and integrate recommendations into exploration requirements & ops. In short, we propose a system engineering approach to roadmap the necessary experiments, analysis, and modeling up front--rather than try to knit together disparate chunks of data into a sensible conclusion after the fact.

Rucker, Michelle↗

NASA NTRS2015

Life Beyond the Planet of Origin and Implications for the Search for Life on Mars

Outer space is vast, cold, devoid of matter, radiation filled with essentially no gravity. These factors present an environmental challenge for any form of life. Earth's biosphere has evolved for more than 3 billion years shielded from the hostile environment of outer space by the protective blanket of the atmosphere and magnetosphere. Space is a nutritional wasteland with no liquid water and readily available organic carbon. Moving beyond a life's planet of origin requires a means for transport, the ability to withstand transport, and the ability to colonize, thrive and ultimately evolve in the new environment. Can life survive beyond its home planet? The key to answering this question is to identify organisms that first have the ability to withstand space radiation, space vacuum desiccation and time in transit, and second the ability to grow in an alien environment. Within the last 60 years space technology allowed us to transport life beyond Earth's protective shield so we may study, in situ, their responses to selected conditions of space. To date a variety of microbes ranging from viruses, to Bacteria, to Archaea, to Eukarya have been tested in the space environment. Most died instantly, but not all. These studies revealed that ultraviolet radiation is the near-term lethal agent, while hard radiation is the long-term lethal agent when the organism is shielded from ultraviolet radiation. In fact, bacterial spores, halophilic cyanobacteria and Archaea as well as some lichens survive very well if protected from ultraviolet radiation [1]. Some microbes, then, may be able to survive the trip in outer space to Mars on a spacecraft or in a meteorite. Once on Mars can a terrestrial microbe survive? Although the conditions on Mars are not as harsh as those in space, they are not hospitable for a terrestrial microbe. Studies, however, have shown that certain microbes that can survive in space for several years may also be able to survive on Mars if protected from ultraviolet radiation [1]. Laboratory simulation experiments using a mock-up of the Phoenix lander have shown that microbes transported to the surface of Mars on a spacecraft come off the spacecraft and mix into the Martian regolith [2]. Additionally, studies simulating Martian dust storms demonstrate that microbes can survive in the Martian wind blown dust and be scattered across the Martian surface away from the spacecraft. Would these microbes that may survive on Mars metabolize and propagate? Growth requires liquid water, a carbon source and an energy source. Survival on Mars also requires protection from ultraviolet radiation. In the cold, dry environment of Mars the probability of microbial metabolism and growth at or just beneath the surface is extremely low. Although the probability is low, Mars may be contaminated with potentially live terrestrial organisms. In light of that statistic we must be extremely diligent and cautious in our search for Martian life. If we are not cautious we may find life on Mars and it may be a contaminant from Earth.

Survival in Space↗

NASA NTRS2017

Airborne Hyperspectral Sensing of Monitoring Harmful Algal Blooms in the Great Lakes Region: System Calibration and Validation

Harmful algal blooms (HABs) in Lake Erie have been prominent in recent years. The bloom in 2014 reached a severe level causing the State of Ohio to declare a state of emergency. At that time NASA Glenn Research Center was requested by stakeholders to help monitor the blooms in Lake Erie. Glenn conducted flights twice a week in August and September and assembled and distributed the HAB information to the shoreline water resource managers using its hyperspectral imaging sensor (in development since 2006), the S􀂱3 Viking aircraft, and funding resources from the NASA Headquarters Earth Science Division. Since then, the State of Ohio, National Oceanic and Atmospheric Administration (NOAA), and U.S. Environmental Protection Agency (EPA) have elevated their funding and activities for observing, monitoring, and addressing the root cause of HABs. Also, the communities and stakeholders have persistently requested NASA Glenn􀂶s participation in HAB observation. Abundant field campaigns and sample analyses have been funded by Ohio and NOAA, which provided a great opportunity for NASA to advance science and airborne hyperspectral remote sensing economically. Capitalizing on this opportunity to advance the science of algal blooms and remote sensing, NASA Glenn conducted the Airborne Hyperspectral Observation of harmful algal blooms campaign in 2015 that was, in many respects, twice as large as the 2014 campaign. Focusing mostly on Lake Erie, but also including other small inland lakes and the Ohio River, the campaign was conducted in partnership with a large number of partners specializing in marine science and remote sensing. Airborne hyperspectral observation of HABs holds promise to distinguish potential HABs from nuisance blooms, determine their concentrations, and delineate their movement in an augmented spatial and temporal resolution and under clouds􀂲all of which are excellent complements to satellite observations. Working with collaborators at several Ohio and Michigan institutions as well as one in South Dakota and one in Alabama, this effort was able to provide next-day georeferenced estimates of cyanobacteria and scum concentrations. Very prompt processing and analysis of the hyperspectral imagery is necessary for the information to be acted upon. For example, a next-day report of an overflight over the Ohio River indicated that a bloom could be present as far downstream as the Cincinnati intake, but the Ohio EPA had not received visual reports of a bloom that far downstream. Water samples were obtained at the Cincinnati water intake, based on the flight data, and detected microcystins in the source water. The flight data helped State and municipal authorities realize the potential extent of that bloom, and triggered response sampling, before the visual river-wide scums started forming. The present document describes the process that was utilized to take raw remote sensing data and create information products; this includes system calibration and validation, efforts to correct atmospheric effects, and algorithms that produce the data products. Furthermore, successful research into improved algorithms for expanding the capability to delineate in water constituents is included. Finally, comparisons that show expected relationships between ground-based measurements and hyperspectral imager version 2 (HSI2) data results are presented, giving confidence in the remote sensing products.

Hyperspectral Imaging↗

NASA NTRS2017

Simulating PACE Global Ocean Radiances

The NASA PACE mission is a hyper-spectral radiometer planned for launch in the next decade. It is intended to provide new information on ocean biogeochemical constituents by parsing the details of high resolution spectral absorption and scattering. It is the first of its kind for global applications and as such, poses challenges for design and operation. To support pre-launch mission development and assess on-orbit capabilities, the NASA Global Modeling and Assimilation Office has developed a dynamic simulation of global water-leaving radiances, using an ocean model containing multiple ocean phytoplankton groups, particulate detritus, particulate inorganic carbon (PIC), and chromophoric dissolved organic carbon (CDOC) along with optical absorption and scattering processes at 1 nm spectral resolution. The purpose here is to assess the skill of the dynamic model and derived global radiances. Global bias, uncertainty, and correlation are derived using available modern satellite radiances at moderate spectral resolution. Total chlorophyll, PIC, and the absorption coefficient of CDOC (aCDOC), are simultaneously assimilated to improve the fidelity of the optical constituent fields. A 5-year simulation showed statistically significant (P < 0.05) comparisons of chlorophyll (r = 0.869), PIC (r = 0.868), and a CDOC (r =0.890) with satellite data. Additionally, diatoms (r = 0.890), cyanobacteria (r = 0.732), and coccolithophores (r = 0.716) were significantly correlated with in situ data. Global assimilated distributions of optical constituents were coupled with a radiative transfer model (Ocean-Atmosphere Spectral Irradiance Model, OASIM) to estimate normalized water-leaving radiances at 1 nm for the spectral range 250-800 nm. These unassimilated radiances were within 0.074 mW/sq cm/micron/sr of MODIS-Aqua radiances at 412, 443, 488, 531, 547, and 667 nm. This difference represented a bias of 10.4% (model low). A mean correlation of 0.706 (P < 0.05) was found with global distributions of MODIS radiances. These results suggest skill in the global assimilated model and resulting radiances. The reported error characterization suggests that the global dynamical simulation can support some aspects of mission design and analysis. For example, the high spectral resolution of the simulation supports investigations of band selection. The global nature of the radiance representations supports investigations of satellite observing scenarios. Global radiances at bands not available in current and past missions support investigations of mission capability. PACE, ocean color, water-leaving radiances, biogeochemical model, radiative transfer model

PIC↗