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At least 235 records · Page 13

Ablating Pellets for Areal Density Symmetry Control in Indirectly Driven Inertial Confinement Fusion Target Designs

Asymmetry of the compressed fusion fuel configuration, as characterized by a variation in areal density, in inertial confinement fusion (ICF) implosions is a known performance limitation. While ignition has been achieved on the National Ignition Facility (NIF), implosion symmetry was not perfect, and subsequent experiments that have extended fusion performance still struggle with symmetry control and would, in principle, perform much better if the areal density uniformity of the fuel was improved by eliminating thin regions in the DT (Deuterium-Tritium) fuel that develop by the time of peak compression. In indirect drive (IDD), altering the time-dependent laser cone fraction and cross-beam energy transfer are the usual tactics for controlling implosion asymmetry in IDD target designs, but it may be that those tools have been pushed to their limits. In this Letter, we present a new tactic for controlling the implosion using satellite ablating pellets to manipulate the time-dependent x-ray field around an ICF capsule. In theory and in simulations the action of these pellets is quite effective at reducing the swing of x-ray drive from waist hot to pole hot, thus achieving a more one-dimensional fuel configuration at peak compression. Simulations show that a factor of ∼2 in yield performance can be obtained via this technique, when applied to past record implosions on the NIF. As a result, some speculative alternate uses of the proposed target concept are also mentioned in the conclusion of this Letter.

Implosion symmetry↗

Comparison of Extended and Accelerated VOG Tests

The reprocessing of used nuclear fuel would release volatile radionuclides into the off-gas streams of a processing plant, including ¹²⁹I. The dissolver off-gas and more dilute vessel off-gas streams (VOG) must both be targeted when mitigating ¹²⁹I environmental release because each contains some amount of iodine. Iodine-129 will likely be found as elemental iodine (I₂) and methyl iodide (CH₃I) in these off-gas streams. Reduced silver-exchanged mordenite (AgZ or Ag⁰Z) has been investigated as a potential sorbent for iodine abatement and is studied here under prototypic VOG conditions. Because of the relatively low iodine abundance and high flow rates of the VOG, total iodine concentrations are expected to be in the parts per billion range. Thus, VOG experiments need to run for extended durations at low concentrations to reach sorbent saturation. Because the sorbent will be exposed to oxidizing gas streams for extended periods of time, aging effects and sorbent degradation need to be considered when designing a sorbent-based abatement system. Three sets of experiments were performed with the aim of determining how the adsorption of iodine by AgZ is affected by differing test durations and gas compositions. The first tested the capacity of sorbent aged for 8 months under a humid air stream. The second tested differences in sorbent capacity and mass transfer zone (MTZ) length during high-concentration (1200 ppb v ) CH₃I loading in a humid nitrogen gas (N₂) stream and a humid air gas stream over 28 days. The third tested sorbent capacity and MTZ length during low concentration (< 200 ppb v ) CH₃I and I₂ loading in a humid air stream over 9 months. The results of these tests suggest that AgZ capacity drops by ~50% after 1 month of aging, by ~60% after 2 months of aging, and then does not continue to decrease significantly at up to 8 months of aging. One-month aging tests that compared N₂ and air as the gas stream diluent resulted in similar maximum loading capacities and overall loading curves, indicating that the effects of aging cannot be mitigated by avoiding air as the balance gas. The 9-month extended VOG tests did not result in clear sorbent saturation at the inlet, but it can be inferred using an assumption of a maximum capacity of 45 mg I/g AgZ. Applying this assumption, then the MTZ of CH₃I is 12.8 cm, and the MTZ of I₂ is 12.4 cm. These results are similar to previous tests. Comparisons to tests completed at Idaho National Laboratory suggest that the CH₃I MTZ may be dependent on concentration. Future work should re-evaluate the design of the VOG iodine capture system with this updated data and should seek to understand fundamental characteristics of CH₃I adsorption by AgZ, such as the effect of concentration, gas velocity, and gas composition.

11 NUCLEAR FUEL CYCLE AND FUEL MATERIALS↗

The extended ‘stellar halo’ of the Ursa Minor dwarf galaxy

Stellar candidates in the Ursa Minor (UMi) dwarf galaxy have been found using a new Bayesian algorithm applied to Gaia EDR3 data. Five of these targets are located in the extreme outskirts of UMi, from ∼5 to 12 elliptical half-light radii (r h ), where r h (UMi) = 17.32 ± 0.11 arcmin, and have been observed with the high-resolution Gemini Remote Access to CFHT ESPaDOnS Spectrograph at the Gemini North telescope. Precise radial velocities (σ RV < 2 km s −1 ) and metallicities (σ [Fe/H] < 0.2 dex) confirm their memberships of UMi. Detailed analysis of the brightest and outermost star (Target 1, at ∼12r h ), yields precision chemical abundances for the α (Mg, Ca, and Ti), odd-Z (Na, K, and Sc), Fe-peak (Fe, Ni, and Cr), and neutron-capture (Ba) elements. With data from the literature and APOGEE data release 17, we find the chemical patterns in UMi are consistent with an outside-in star-formation history that includes yields from core-collapse supernovae, asymptotic giant branch stars, and Type Ia supernovae. Evidence for a knee in the [α/Fe] ratios near [Fe/H] ∼ −2.1 indicates a low star-formation efficiency similar to that in other dwarf galaxies. Detailed analysis of the surface number density profile shows evidence that UMi’s outskirts have been populated by tidal effects, likely as a result of completing multiple orbits around the Galaxy.

79 ASTRONOMY AND ASTROPHYSICS↗

Object-space optimization of tomographic reconstructions for additive manufacturing

Volumetric 3D printing motivated by computed axial lithography enables rapid printing of homogeneous parts but requires a high dimensionality gradient-descent optimization to calculate image sets. Here we introduce a new, simpler approach to image-computation that algebraically optimizes a model of the printed object, significantly improving print accuracy of complex parts under imperfect material and optical precision by improving optical dose contrast between the target and surrounding regions. Quality metrics for volumetric printing are defined and shown to be significantly improved by the new algorithm. The approach is extended beyond binary printing to grayscale control of conversion to enable functionally graded materials. The flexibility of the technique is digitally demonstrated with realistic projector point spread functions, printing around occluding structures, printing with restricted angular range, and incorporation of materials chemistry such as inhibition. Finally, simulations show that the method facilitates new printing modalities such as printing into flat, rather than cylindrical packages to extend the applications of volumetric printing.

36 MATERIALS SCIENCE↗

The galaxy–halo connection of emission-line galaxies in IllustrisTNG

ABSTRACT We employ the hydrodynamical simulation IllustrisTNG-300-1 to explore the halo occupation distribution (HOD) and environmental dependence of luminous star-forming emission-line galaxies (ELGs) at z ∼ 1. Such galaxies are key targets for current and upcoming cosmological surveys. We select model galaxies through cuts in colour–colour space allowing for a direct comparison with the Extended Baryon Oscillation Spectroscopic Survey and the Dark Energy Spectroscopic Instrument (DESI) surveys and then compare them with galaxies selected based on specific star formation rate (sSFR) and stellar mass. We demonstrate that the ELG populations are twice more likely to reside in lower density regions (sheets) compared with the mass-selected populations and twice less likely to occupy the densest regions of the cosmic web (knots). We also show that the colour-selected and sSFR-selected ELGs exhibit very similar occupation and clustering statistics, finding that the agreement is best for lower redshifts. In contrast with the mass-selected sample, the occupation of haloes by a central ELG peaks at ∼20 per cent. We furthermore explore the dependence of the HOD and the autocorrelation on environment, noticing that at fixed halo mass, galaxies in high-density regions cluster about 10 times more strongly than low-density ones. This result suggests that we should model carefully the galaxy–halo relation and implement assembly bias effects into our models (estimated at ∼4 per cent of the clustering of the DESI colour-selected sample at z = 0.8). Finally, we apply a simple mock recipe to recover the clustering on large scales (r ≳ 1 Mpc h−1) to within 1 per cent by augmenting the HOD model with an environment dependence, demonstrating the power of adopting flexible population models.

79 ASTRONOMY AND ASTROPHYSICS↗

The SPTpol Extended Cluster Survey

We describe the observations and resultant galaxy cluster catalog from the 2770 deg2 SPTpol Extended Cluster Survey (SPT-ECS). Clusters are identified via the Sunyaev–Zel'dovich (SZ) effect and confirmed with a combination of archival and targeted follow-up data, making particular use of data from the Dark Energy Survey (DES). With incomplete follow-up we have confirmed as clusters 244 of 266 candidates at a detection significance ξ ≥ 5 and an additional 204 systems at 4 < ξ < 5. The confirmed sample has a median mass of ${M}_{500c}\sim 4.4\times {10}^{14}\,{M}_{\odot }\,{h}_{70}^{-1}$ and a median redshift of z = 0.49, and we have identified 44 strong gravitational lenses in the sample thus far. Radio data are used to characterize contamination to the SZ signal; the median contamination for confirmed clusters is predicted to be ~1% of the SZ signal at the ξ > 4 threshold, and <4% of clusters have a predicted contamination >10% of their measured SZ flux. We associate SZ-selected clusters, from both SPT-ECS and the SPT-SZ survey, with clusters from the DES redMaPPer sample, and we find an offset distribution between the SZ center and central galaxy in general agreement with previous work, though with a larger fraction of clusters with significant offsets. Adopting a fixed Planck-like cosmology, we measure the optical richness–SZ mass ($\lambda -M$) relation and find it to be 28% shallower than that from a weak-lensing analysis of the DES data—a difference significant at the 4σ level—with the relations intersecting at λ = 60. The SPT-ECS cluster sample will be particularly useful for studying the evolution of massive clusters and, in combination with DES lensing observations and the SPT-SZ cluster sample, will be an important component of future cosmological analyses.

79 ASTRONOMY AND ASTROPHYSICS↗

Search for inelastic dark matter-nucleus scattering with the PICO-60 CF$_{3}$I and C$_{3}$F$_{8}$ bubble chambers

PICO bubble chambers have exceptional sensitivity to inelastic dark matter-nucleus interactions due to a combination of their extended nuclear recoil energy detection window from a few keV to $O$(100 keV) or more and the use of iodine as a heavy target. Inelastic dark matter-nucleus scattering is interesting for studying the properties of dark matter, where many theoretical scenarios have been developed. This study reports the results of a search for dark matter inelastic scattering with the PICO-60 bubble chambers. The analysis reported here comprises physics runs from PICO-60 bubble chambers using CF$_{3}$I and C$_{3}$F$_{8}$. The CF$_{3}$I run consisted of 36.8 kg of CF$_{3}$I reaching an exposure of 3415 kg-day operating at thermodynamic thresholds between 7 and 20 keV. The C$_{3}$F$_{8}$ runs consisted of 52 kg of C$_{3}$F$_{8}$ reaching exposures of 1404 kg-day and 1167 kg-day running at thermodynamic thresholds of 2.45 keV and 3.29 keV, respectively. The analysis disfavors various scenarios, in a wide region of parameter space, that provide a feasible explanation of the signal observed by DAMA, assuming an inelastic interaction, considering that the PICO CF$_{3}$I bubble chamber used iodine as the target material.

72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS↗

Validation of Hadron Mass Correction Schemes in Deep Inelastic Scattering at Low Energy Transfer

One method of describing the quark-gluon of the structure proton relies on factorizing its cross-section into a process-dependent short distance element and an independent long distance element that quantifies the quark and gluon momentum distributions within the proton. This research focuses on validating the kinematic range at which factorization theorems still accurately describe the cross-section, in particular for events that simulate scattering on large momentum quarks, that are sensitive to the details of the color mechanism. In this regime, effects which otherwise scale away at large energies, become non-negligible at low energies. The goal of this research is to assess the viability of an extended version of ?collinear factorization? recently proposed by J. Guerrero and A. Accardi, which includes kinematic corrections at quark level, known as ?hadron mass corrections?, to account for the masses of the quarks and the target. To accomplish this, deep inelastic scattering events are simulated using a realistic model of deep inelastic scattering, in which one may analytically calculate both the structure functions and the quark distribution functions. These simulated experiments are then used to test the viability of the proposed factorization theorem by fitting the quark distributions and comparing these to the analytically calculable ones. It is found that the viability of hadron mass corrections in low energy scattering experiments, such as those at Jefferson Lab, extends beyond the kinematic range suggested by analytic calculations alone, provided that residual higher twist corrections are simultaneously fitted to the data.

Krause, Andrew↗

Cybersecurity of Wide Area Monitoring, Protection and Control Systems for HVDC Applications

The flexibility provided by High Voltage Direct Current (HVDC) systems can be further extended by Wide Area Monitoring, Protection, and Control (WAMAPC) systems. WAMPAC systems enable many HVDC applications and, on the other hand, inevitably introduce cybersecurity concerns that need to be addressed. In this work, a security domain layer and decision framework is reported to detect and mitigate the impact of false data injection (FDI) attacks targeting HVDC stations. Specifically, a rule-based cyber-attack detection method is introduced and implemented on Raspberry Pi and tested on the real-time HVDC simulation facility on the real-time digital simulator (RTDS) platform at ABB U.S. Corporate Research Center.

cybersecurity↗

Ultrafast vibrational control of organohalide perovskite optoelectronic devices using vibrationally promoted electronic resonance

Vibrational control (VC) of photochemistry through the optical stimulation of structural dynamics is a nascent concept only recently demonstrated for model molecules in solution. Extending VC to state-of-the-art materials may lead to new applications and improved performance for optoelectronic devices. Metal halide perovskites are promising targets for VC due to their mechanical softness and the rich array of vibrational motions of both their inorganic and organic sublattices. Here, we demonstrate the ultrafast VC of FAPbBr 3 perovskite solar cells via intramolecular vibrations of the formamidinium cation using spectroscopic techniques based on vibrationally promoted electronic resonance. The observed short (~300 fs) time window of VC highlights the fast dynamics of coupling between the cation and inorganic sublattice. First-principles modelling reveals that this coupling is mediated by hydrogen bonds that modulate both lead halide lattice and electronic states. Cation dynamics modulating this coupling may suppress non-radiative recombination in perovskites, leading to photovoltaics with reduced voltage losses.

36 MATERIALS SCIENCE↗

Exploiting user activeness for data retention in HPC systems

HPC systems typically rely on the fixed-lifetime (FLT) data retention strategy, which only considers temporal locality of data accesses to parallel file systems. However, our extensive analysis based on the leadership-class HPC system traces suggests that the FLT approach often fails to capture the dynamics in users' behavior and leads to undesired data purge. In this study, we propose an activeness-based data retention (ActiveDR) solution, which advocates considering the data retention approach from a holistic activeness-based perspective. By evaluating the frequency and impact of users' activities, ActiveDR prioritizes the file purge process for inactive users and rewards active users with extended file lifetime on parallel storage. Our extensive evaluations based on the traces of the prior Titan supercomputer show that, when reaching the same purge target, ActiveDR achieves up to 37% file miss reduction as compared to the current FLT retention methodology.

Zhang, Wei↗

Improving Dark Energy Constraints Using Low-Redshift Large-Scale Structures

The primary goal of this project was to improve constraints on dark energy measurements by improving our ability to extract cosmological information from low redshift large-scale structures. PI Clowe's project's primary aim was to reduce the bias in measurements of the masses of clusters of galaxies to a level where the evolution of the cluster mass function can be used in the Vera Rubin Observatory's Legacy Survey of Space and Time Dark Energy Science Collaboration survey to improve the accuracy of the measurement of dark energy and other cosmological parameters. Co-PI Seo's project studied observational systematics affecting large-scale clustering of galaxies, which will be used to improve dark energy constraints from the Dark Energy Spectroscopic Instrument (DESI). The cluster lensing project employed a series of simulations and observations of clusters of galaxies to test numerous potential systematic errors in cluster mass measurements using weak gravitational lensing as the accuracy of current weak lensing measurements are more than order of magnitude worse than what is required to use clusters of galaxies for accurate determination of dark energy parameters. PI Clowe and group developed and analyzed simulations to test for, and correct biases introduced in, the weak lensing measurement process. Finally, PI Clowe and group developed a method of detecting clusters using galaxy overdensities and applied the method to the BLISS and DES surveys. The success of spectroscopic dark energy mission such as the extended Baryon Oscillation Spectroscopic Survey (eBOSS) and the Dark Energy Spectroscopic Instrument (DESI) will depend on a thorough understanding of various observational systematics in the target density fluctuations that would give rise to spurious, non-cosmological signals. PI Seo and group developed a deep learning, artificial neural network (ANN) technique that modeled and mitigated such effects, aimed at deriving more robust galaxy clustering signals not only for the baryon acoustic oscillation feature and redshift-space distortions but also for primordial non-Gaussianity constraint.

79 ASTRONOMY AND ASTROPHYSICS↗

Investigation of boosted proton energies through proton radiography of target normal sheath acceleration fields in the multi-ps regime

Multi-kilojoule, multi-picosecond short-pulse lasers, such as the National Ignition Facility-Advanced Radiographic Capability laser and the OMEGA-Extended Performance laser, which have been constructed over the last two decades, enable exciting opportunities to produce high-brightness, high-energy laser-driven proton sources for applications in high-energy-density science like proton fast ignition for inertial fusion energy, particle radiography, and materials science studies. Results on these platforms have demonstrated enhanced accelerated proton energies and electron temperatures when compared to established scaling laws. Recent work has developed a new scaling for proton TNSA in the multi-ps regime. However, this new physics in the multi-ps regime motivates the need to understand the origin of the enhancement in proton energies. Toward this goal, here this work presents the first measurements of the TNSA accelerating sheath field in the multi-ps regime for pulse durations of 0.6, 5, and 10 ps. This measurement was achieved by using a separate TNSA proton source to radiograph the spatiotemporal profile of the accelerating sheath that is responsible for proton acceleration. The use of stacked radiochromic film detectors allows for a discrete time profile of the radiographs, thus enabling the measurement of the temporal and spatial evolution of the accelerating field. In performing this measurement, we extract quantities such as the sheath strength as a function of time and pulse duration, which shows that longer pulse durations sustain a stronger electric field for a longer duration when compared to sub-ps laser pulses, which may enable the observed boosted proton energies and proton conversion efficiencies.

70 PLASMA PHYSICS AND FUSION TECHNOLOGY↗

Measurement of the Neutron Elastic Electromagnetic Form Factor Ratio at Large Momentum Transfer

Exploring nucleon structure is vital both for understanding its origin and existence as well as for the advancement of the sciences. It helps us answer key questions such as how quark and gluon dynamics create 99% of the nucleon mass. Electron- nucleon scattering has been widely used for precision studies of the nucleon and nuclear structure since the Nobel Prize winning investigations by Robert Hofstadter and collaborators in the 1950s. These studies provide information about the spatial charge and current densities of the nucleon in terms of the electromagnetic form factors. The form factors are functions of four momentum transfer squared (Q2). Extending the electromagnetic form factor measurements to higher Q2 plays a critical role in furthering the understanding of nucleon structure. This motivated the Super BigBite Spectrometer (SBS) program at Jefferson Lab. The open nature of the spectrometers and the direct line of sight from the target to the tracking detector locations in experimental setups such as SBS creates high levels of background at the detectors. This necessitates the use of tracking detectors with high rate capability and good position resolution. Gas Electron Multiplier (GEM) detectors are an excellent choice for tracking detectors in such experiments. Understanding the performance of the GEM detectors is important not just for SBS experiments but also for future high-luminosity experiments. This thesis reports the exploratory results from the measurement of the neutron elastic electromagnetic form factor ratio (Gn E/Gn M) at high momentum transfer. A longitudinally polarized electron beam was scattered off a polarized 3He target, used as an effective polarized neutron target. In this experiment, the polarized 3He target achieved a world record polarization weighted luminosity at a beam current of 45 µA. Double spin asymmetry of the scattered neutron events is used to extract the neutron form factor ratio. Measurements were taken at Q2 = 3.0, 6.8, 9.8 (GeV/c)2. The lowest Q2 measurement is in good agreement with the existing world data, and the higher-Q2 measurements extend the Q2 reach well beyond the existing world data and are expected to remain unmatched for a long time.

Gamage, Vimukthi Haththotuwa [Univ. of Virginia, C↗

Using Hardware-In-The-Loop Methodology to Develop Test Systems

Hardware in the Loop (HIL) testing methodologies have become widespread in industry. Typically, they focus on developing control algorithms for systems such as autonomous vehicles or aircraft. An oft overlooked aspect of product development is the design and fabrication of a test system for validating that the product meets requirements. Abstractly, a test system differs little from a control system—testers provide signals to the unit, monitor feedback, and base decisions on the results. While the time scales may differ, the functionalities are conceptually similar. Viewed in this light, it becomes natural to extend HIL approaches to tester development. By replacing a physical unit with a proxy model deployed to a real-time or pseudo real-time target, test systems can be developed in parallel with the design and fabrication of a first production unit. This saves considerable time in the life cycle from conceptual design to realized product. This manuscript demonstrates the process flow using a capacitive discharge unit as an exemplar.

42 ENGINEERING↗

Extending the Dynamic Range of Galaxy Outflow Scaling Relations: Massive Compact Galaxies with Extreme Outflows

We investigate galactic winds in the HizEA galaxies, a collection of 46 late-stage galaxy mergers at z = 0.4–0.8, with stellar masses of $\mathrm{log}({M}_{* }/{M}_{\odot })=10.4\mbox{--}11.5$ , star formation rates (SFRs) of 20–500 M ⊙ yr -1 , and ultra-compact (a few 100 pc) central star-forming regions. We measure their gas kinematics using the Mg II λλ 2796,2803 absorption lines in optical spectra from MMT, Magellan, and Keck. We find evidence of outflows in 90% of targets, with maximum outflow velocities of 550–3200 km s -1 . We combine these data with ten samples from the literature to construct scaling relations for outflow velocity versus SFR, star formation surface density (Σ SFR ), M * , and SFR/M * . The HizEA galaxies extend the dynamic range of the scaling relations by a factor of ~2–4 in outflow velocity and an order of magnitude in SFR and Σ SFR . The ensemble scaling relations exhibit strong correlations between outflow velocity, SFR, SFR/R, and Σ SFR , and weaker correlations with M * and SFR/M * . The HizEA galaxies are mild outliers on the SFR and M* scaling relations, but they connect smoothly with more typical star-forming galaxies on plots of outflow velocity versus SFR/R and Σ SFR . These results provide further evidence that the HizEA galaxies' exceptional outflow velocities are a consequence of their extreme star formation conditions rather than hidden black hole activity, and they strengthen previous claims that Σ SFR is one of the most important properties governing the velocities of galactic winds.

79 ASTRONOMY AND ASTROPHYSICS↗

Electron acceleration at oblique angles via stimulated Raman scattering at laser irradiance >10 16 W cm –2 μm 2

The generation of hot, directional electrons via laser-driven stimulated Raman scattering (SRS) is a topic of great importance in inertial confinement fusion (ICF) schemes. Little recent research has been dedicated to this process at high laser intensity, in which back, side, and forward scatter simultaneously occur in high energy density plasmas, of relevance to, for example, shock ignition ICF. In this paper, we present an experimental and particle-in-cell (PIC) investigation of hot electron production from SRS in the forward and near-forward directions from a single speckle laser of wavelength λ 0 = 1.053μm, peak laser intensities in the range I 0 = 0.2–1.0×10 17 Wcm –2 and target electron densities between ne = 0.3–1.6% n c , where n c is the plasma critical density. As the intensity and density are increased, the hot electron spectrum changes from a sharp cutoff to an extended spectrum with a slope temperature T = 34 ± 1 keV and maximum measured energy of 350 keV experimentally. Multidimensional PIC simulations indicate that the high energy electrons are primarily generated from SRS-driven electron plasma wave phase fronts with k vectors angled ~50° with respect to the laser axis. These results are consistent with analytical arguments that the spatial gain is maximized at an angle which balances the tendency for the growth rate to be larger for larger scattered light wave angles until the kinetic damping of the plasma wave becomes important. The efficiency of generated high energy electrons drops significantly with a reduction in either laser intensity or target electron density, which is a result of the rapid drop in growth rate of Raman scattering at angles in the forward direction.

75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND↗

Turbo‐charging crop improvement: harnessing multiplex editing for polygenic trait engineering and beyond

Multiplex CRISPR editing has emerged as a transformative platform for plant genome engineering, enabling the simultaneous targeting of multiple genes, regulatory elements, or chromosomal regions. This approach is effective for dissecting gene family functions, addressing genetic redundancy, engineering polygenic traits, and accelerating trait stacking and de novo domestication. Its applications now extend beyond standard gene knockouts to include epigenetic and transcriptional regulation, chromosomal engineering, and transgene‐free editing. These capabilities are advancing crop improvement not only in annual species but also in more complex systems such as polyploids, undomesticated wild relatives, and species with long generation times. At the same time, multiplex editing presents technical challenges, including complex construct design and the need for robust, scalable mutation detection. We discuss current toolkits and recent innovations in vector architecture, such as promoter and scaffold engineering, that streamline workflows and enhance editing efficiency. High‐throughput sequencing technologies, including long‐read platforms, are improving the resolution of complex editing outcomes such as structural rearrangements—often missed by standard genotyping—when targeting repetitive or tandemly spaced loci. To fully realize the potential of multiplex genome engineering, there is growing demand for user‐friendly, synthetic biology‐compatible, and scalable computational workflows for gRNA design, construct assembly, and mutation analysis. Experimentally validated inducible or tissue‐specific promoters are also highly desirable for achieving spatiotemporal control. As these tools continue to evolve, multiplex CRISPR editing is poised to become a foundational technology of next‐generation crop improvement to address challenges in agriculture, sustainability, and climate resilience.

59 BASIC BIOLOGICAL SCIENCES↗