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At least 235 records · Page 13

Current NASA In-Situ Resource Utilization (ISRU) Strategic Vision

Perform development to TRL 5/6 through ground demonstration in relevant environment. Perform component/subscale subsystem flight demonstrations on small/mid-size landers. Assess and characterize water in volatiles in lunar polar shadowed regions and craters. Reduce risk of ISRU for mission critical consumables through Integrated End-to-End Flight Demonstrations (pilot scale). Establish initial Human Mission Scale production capability to promote sustainable operations and as anchor for commercial involvement. Identify and characterize polar region environment and resources/volatiles for Science and future Exploration/Commercial applications. Provide ground-truth physical, mineral, and water/volatile resource characteristic information at multiple locations to provide geological context for science-focused theories of volatile placement and initial mining assessments.Test technologies and processes to reduce risk of future extraction/mining systems. Quantify concentration and lateral/vertical distribution of resources/volatiles. Utilize ISRU capabilities to Extend and Enhance Human Lunar Exploration Missions. Provide oxygen (and fuel) to enable reusable human lunar lander (10+ MT/yr O2)Process carbon-based crew waste/trash into gases and propellants; can reduce logistics while minimizing public perception issues (alternative is conversion to radiation shielding). Scavenge unused propellants and hardware from spent landers. Metal extraction from regolith as feedstock for in situ and in space manufacturing demonstrations. Civil engineering and construction aimed at future outpost/infrastructure build-up. Develop and Demonstrate ISRU for Human Mars Missions. ISRU for propellant production (10-15 MT/yr); Liquefy, store, transfer, and refuel ascent vehicle. Use Moon for operational experience and mission validation for Mars: Pre-deployment & remote activation and operation without crew. Storing and transferring mission consumables Landing crew with empty tanks with ISRU propellants already made and waiting. Support/Promote Commercialization of Space. Large scale polar ice mining (100+ MT/yr water)O2/H2 propulsion for landers/cis-lunar transportation with surface and in space depots. In situ construction and energy expansion at mining and human outpost site(s). ISRU Ground Development. Develop and advance ISRU technologies to enable acquisition of resources and processing into mission consumables. Utilize Multi-center collaboration with a portfolio that includes internal NASA work, external contracts, and collaborative agreements/partnerships. Where appropriate, develop lunar ISRU components and subsystems with a Mars-forward application. Engage industry through public-private partnerships to lay the foundation for long-term lunar and space economic development. Spin-in/spin-out technologies for terrestrial applications and industry (mining, oil & gas, alternative energy, construction). Flight Demonstration Path to Operational ISRU. Utilize small demonstrations with near off-the-shelf hardware to obtain critical information quickly on lunar resources and operations. Demonstrate critical technologies and processes that interact with lunar materials and environments. Perform 'pilot plant' demonstrations at architecture relevant scales and durations to reduce the risk for ISRU-provided products for critical human mission applications.

In-situ Resource Utilization↗

pH adjustment increases biofuel production from inhibitory switchgrass hydrolysates

Biofuels derived from renewable and sustainable lignocellulosic biomass, such as switchgrass, offer a promising means to limit greenhouse gas emissions. However, switchgrass grown under drought conditions contains high levels of chemical compounds that inhibit microbial conversion to biofuels. Fermentation of drought switchgrass hydrolysates by engineered Saccharomyces cerevisiae and Zymomonas mobilis results in lower ethanol production than does fermentation of hydrolyzed switchgrass from a typical rainfall year. Here, it is demonstrated that this inhibitory effect can be alleviated by altering the pH of drought switchgrass hydrolysates produced by two different pretreatment methods: Ammonia Fiber Expansion (AFEX) and Soaking in Aqueous Ammonia (SAA). Fermentation rates and biofuel production by Saccharomyces cerevisiae and Zymomonas mobilis were higher at pH 5.8 than at pH 5.0 from all feedstock years and following both pretreatment methods. SAA pretreatment of drought switchgrass furthermore enabled increased fermentation rates and biofuel titers compared to AFEX pretreatment. A synthetic mimic of switchgrass hydrolysate was developed and identified relief from pH-dependent inhibition by lignocellulose-derived inhibitors as the cause of increased biofuel production above a pH of 5.0. These results demonstrate that SAA pretreatment and pH adjustment can significantly improve fermentation and biofuel production from inhibitory feedstocks by industrial microorganisms.

09 BIOMASS FUELS↗

Development and Validation of a Process Model and Open-Source Process Simulator for Microalgae-Based Tertiary Phosphorus Recovery

Microalgae-based tertiary wastewater treatment has the potential to meet stringent effluent phosphorus limits, with the added benefit of producing a marketable feedstock. However, the lack of validated mechanistic models and their implementation in process simulators have limited the adoption of this technology. In this study, an updated lumped pathway metabolic model (Phototrophic-Mixotrophic Process Model, PM 2 ), including both photoautotrophic and heterotrophic metabolisms of microalgae, was developed to predict effluent phosphorus concentration and biomass yield in response to dynamic influent and varying environmental conditions. The model was implemented in QSDsan – an open-source, Python-based design and simulation platform – for robust simulation under uncertainty. A global sensitivity analysis was performed to prioritize model parameters for calibration. The model was then calibrated and validated using batch experimental data and 45 days of continuous online monitoring data from a full-scale (568 m 3 ·d -1 ) microalgae-based tertiary wastewater treatment plant (EcoRecover process). In particular, along with dynamic influent composition, temperature and light intensity data with diel variation were provided as model inputs to reflect the microalgal behavior under day-night cycling. Overall, the QSDsan-based microalgae process simulator was able to predict effluent phosphorus within 0.02–0.04 mg-P·L -1 , while also capturing the general trends of state variables according to nutrient availability.

Lumped pathway metabolic model↗

Hydrogen utilization and alternatives

The historical uses of hydrogen are described along with potential new uses which could develop as a result of the diminishing supply of conventional fossil fuels such as natural gas. A perspective view of hydrogen, both as a chemical feedstock and as a fuel, is necessary to understand its relationship to the overall national energy projections. These projections, which show energy usage in terms of use sectors, forms of energy, and sources of energy, do not specifically identify hydrogen as a component of the energy system. By superimposing the traditional roles upon the new opportunities for hydrogen on the energy projections, the role of hydrogen and future projections is developed within the context of the national energy projections. Use, supply, and other factors affecting application are interrelated and are discussed.

Manvi, R.↗

Evaluating the carbon capture potential of industrial waste as a feedstock for enhanced weathering

Abstract Limiting anthropogenic global climate warming since the start of the industrial period to less than 2 °C will very likely require both deep and rapid reductions in anthropogenic greenhouse gas emissions and a range of approaches toward carbon dioxide removal (CDR). One prominent CDR approach is enhanced weathering (EW), in which crushed silicate rock is applied on land or in the open ocean to accelerate natural weathering processes that absorb carbon dioxide from Earth’s ocean–atmosphere system. However, in addition to a range of potential environmental, socioeconomic, and ethical issues associated with this pathway, bottlenecks in feedstock sourcing represent a key barrier for deployment of EW at scale. Here, we evaluate the potential of silicate wastes produced from industrial processes—such as steel slag and cement waste—as feedstocks for the EW process. An empirical model that links industrial alkaline waste production to gross domestic product at purchase power parity is developed to forecast waste production in the alternative futures described by the shared socioeconomic pathway (SSP) framework. By incorporating these results into an intermediate-complexity Earth system model, we also explore the impacts of EW using industrial waste on changes to global temperature, ocean pH, and ocean aragonite saturation state, while also quantifying overall CDR efficiency through the end of the century. We estimate a maximum cumulative end-of-century capture potential of ∼400 GtCO 2 for industrial waste, which could represent a significant fraction of the projected CDR requirement of many mitigation scenarios in the SSP framework. However, feedstock-dependent environmental impacts and the technoeconomics of feedstock redistribution may ultimately limit deployment scope.

Xu, Pengxiao (ORCID:0009000633724293)↗

Measurements of Silicosis Factors in Lunar and Martian Simulants

Simulants are geologically complex materials that are developed to represent the physical and/or compositional characteristics of a planetary surface (e.g., a naturally occurring soil or regolith). There are dozens of commercially available simulants that have been developed over the years; each simulant exhibits unique physical, chemical, and mineralogical characteristics. Simulants are derived from either natural or synthetic sources (i.e., “feedstocks”) of glass, minerals, and rocks. These feedstock components are processed by crushing, pulverizing, melting, etc., and then combined in the appropriate proportions to represent a particular site, surface, or region (e.g., Lunar Highlands Regolith). The process of creating simulants therefore requires the mechanical breakdown and reincorporation of feedstock components which may contain crystalline silica minerals such as quartz, cristobalite, and tridymite. Certain crystalline silica particles of the respirable fraction (<10 μm in diameter) are of great concern; chronic and acute exposure to these respirable crystalline silica (RCS) can lead to permanent damage and scarring of lung tissue, incurable lung diseases (i.e., silicosis), lung cancer, COPD (chronic obstructive pulmonary disease), and kidney disease. Planetary simulants are used extensively as test materials in the in scientific and engineering communities (e.g., testing of dust mitigation technologies, in-situ resource utilization, rover mobility, hardware, soft goods etc.). As such, this assessment was developed to serve as a guide for simulant users, local Safety and Occupational Health professionals, and Industrial Hygienists to evaluate the risk of silicosis across a wide variety of Lunar and Martian simulants. The goal of these works is to ensure that those working with simulant can do so safely and with an informed understanding of potential health risks.

Lunar↗

Measurements of Silicosis Factors in Lunar and Martian Simulants

Simulants are geologically complex materials that are developed to represent the physical and/or compositional characteristics of a planetary surface (e.g., a naturally occurring soil or regolith). There are dozens of commercially available simulants that have been developed over the years; each simulant exhibits unique physical, chemical, and mineralogical characteristics. Simulants are derived from either natural or synthetic sources (i.e., “feedstocks”) of glass, minerals, and rocks. These feedstock components are processed by crushing, pulverizing, melting, etc., and then combined in the appropriate proportions to represent a particular site, surface, or region (e.g., Lunar Highlands Regolith). The process of creating simulants therefore requires the mechanical breakdown and reincorporation of feedstock components which may contain crystalline silica minerals such as quartz, cristobalite, and tridymite. Certain crystalline silica particles of the respirable fraction (<10 μm in diameter) are of great concern; chronic and acute exposure to these respirable crystalline silica (RCS) can lead to permanent damage and scarring of lung tissue, incurable lung diseases (i.e., silicosis), lung cancer, COPD (chronic obstructive pulmonary disease), and kidney disease. Planetary simulants are used extensively as test materials in the in scientific and engineering communities (e.g., testing of dust mitigation technologies, in-situ resource utilization, rover mobility, hardware, soft goods etc.). As such, this assessment was developed to serve as a guide for simulant users, local Safety and Occupational Health professionals, and Industrial Hygienists to evaluate the risk of silicosis across a wide variety of Lunar and Martian simulants. The goal of these works is to ensure that those working with simulant can do so safely and with an informed understanding of potential health risks.

Lunar↗

Playing with DIRT: Building the Framework for a Comprehensive In-Situ Soil Materials Testing Database

Long-term, sustainable planetary exploration will require the ability to "live off the land," relying on In-Situ Resource Utilization (ISRU) and In-Situ Construction as core capabilities. Reduction of both risk and launch mass for lunar construction will require evaluating and comparing regolith materials for use as feedstocks suitable for in-situ beneficiation and fabrication of building components. These material assessment capabilities will inform lunar infrastructure design decisions, with co-benefits for terrestrial construction using in-situ materials. Current planetary construction technology development relies on lunar mapping and orbital data, Apollo-era sample analyses, current simulant inventories, and tests conducted using analog site soils. Requirements for lunar infrastructure design decisions and construction systems will be determined based on specific environmental conditions, mission architectures, and the materials available within traverse range of lunar feedstock processing depots. Critical for success is the capability to identify, evaluate and make effective use of a wide range of materials as they are found in-situ on the lunar surface. This paper discusses the development of a digital repository for data on soil and regolith properties, beginning with their structural performance in both cementitious and non-cementitious building material formulations. The Database for In-situ Resource Testing (DIRT) compiles a catalog of raw materials, additives, and formulations, with notations pertaining to material sources and preparation techniques entered via a web-based user interface. Design of consistent data schemas for site-sourced materials evaluation will facilitate linkage with relevant terrestrial and planetary materials databases while enabling guided data input via templates for participation by broader groups of collaborators. Results of these analyses are compiled in a centralized repository to generate insights applicable for regolith resources and landing sites yet to be precisely defined. Collection of thorough records of material characteristics, applications and performance will support innovative construction solutions not only for space infrastructure but for sustainable, resilient design in the terrestrial built environment.

Sarah Joey Seitz↗

EBF3 Design and Sustainability Considerations

Electron beam freeform fabrication (EBF3) is a cross-cutting technology for producing structural metal parts using an electron beam and wire feed in a layer-additive fashion. This process was developed by researchers at NASA Langley to specifically address needs for aerospace applications. Additive manufacturing technologies like EBF3 enable efficient design of materials and structures by tailoring microstructures and chemistries at the local level to improve performance at the global level. Additive manufacturing also facilitates design freedom by integrating assemblies into complex single-piece components, eliminating flanges, fasteners and joints, resulting in reduced size and mass. These same efficiencies that permit new design paradigms also lend themselves to supportability and sustainability. Long duration space missions will require a high degree of self-sustainability. EBF3 is a candidate technology being developed to allow astronauts to conduct repairs and fabricate new components and tools on demand, with efficient use of feedstock materials and energy.

Taminger, Karen M. B.↗

Develop a Simulation Framework for Understanding Physico-Chemical Processes and Optimization of CHZ' Plastic Thermolyze Technology (CRADA Final Report)

Thermolyzer(TM) technology is a third-generation, multi-reactor, oxygen-free, low pressure, slow pyrolysis process. It has proven to be remarkably versatile in successfully processing all types of hydrocarbon waste. These include all seven types of plastics, tires, auto shredder residue, carpet, electronic waste, and composites. Working with the IACMI, Thermolyzer(TM) technology has recovered the glass and carbon fibers from wind turbine blades for reuse into new applications. Its primary output is a synthesis gas that is rich in H 2 , CH 4 , and small amounts of C 2-4 aliphatic hydrocarbons. Other components are CO and CO 2 . Three unique features set Thermolyzer(TM) apart from other pyrolysis systems: the syngas is clean enough to run in Siemens or Solar gas turbines or IC engines without fouling, clean, salable, byproducts are produced, and CO 2 emissions are lower than natural gas power plants. Research results have been obtained from an operating 7 ton/day facility. A 44 ton/day plant has successfully operated as noted above. The process works because it makes use of the recoverable embodied energy in the feedstock. Surprisingly, a pound of some plastics has the same recoverable energy content as a pound of gasoline. Thus, it is imperative to develop a process that economically converts scrap plastics (including ocean plastics) into energy and thereby conserve the non-renewable fossil fuels for future generations. The Thermolyzer(TM) technology can also be used to create liquid fuels like gasoline and diesel from hydrocarbon wastes. Because of the high hydrogen content of the synthesis gas, hydrogen can be recovered more inexpensively than the current solar or wind energy being used to electrolyze water. That hydrogen can be used for fuel cell powered vehicles or converted into ammonia for agriculture or as a hydrogen storage medium. Extension of this technology to other wastes such as tires, auto shredder residue or wood wastes would expand the circular economy.

36 MATERIALS SCIENCE↗

Forest residue harvest optimization: spanning the bridge between plant biology and biorefinery performance

Forestry residues have immense potential as alternative feedstocks to petroleum, yet their inherent complexity remains a major challenge to widespread use. Pairing the temporal rhythms of plant biology with biorefinery performance is critical to industrial-scale biorefinery development. Here, we provide the first report of a techno-economic analysis (TEA) and life cycle assessment (LCA) for a model integrated reductive catalytic fractionation (RCF)–molten salt hydrolysis process for forestry residues varying in tree part, species, and phenophase. All forestry residues resulted in net-negative greenhouse gas (GHG) emissions vs. comparable petroleum feedstocks, with GHG emissions potentially reduced >4.0× through composition-based feedstock selection (e.g., harvesting American beech bark in spring vs. summer). Moreover, American beech twigs/branchlets and bark in leafed and emergence phenophases, respectively, had 7.9× lower predicted phenolic minimum selling prices (MSPs) vs. other feedstocks and MSPs within the current global phenolic market range. Hemicellulose content and RCF yield emerged as key parameters impacting GHG emissions and biorefinery revenue, identifying hardwood twigs/branchlets in the leafed phenophase as optimal biofeedstocks. Biorefinery expenses were dominated by purchased equipment, raw materials, and utility costs, highlighting essential areas for future study. Notably, RCF reactor pressures drove 85–90% of equipment costs, but sensitivity analysis revealed that decreasing the pressure 20% could reduce the phenol MSP 4-fold. Structural carbohydrate dynamics were also investigated using a two-step acid hydrolysis method to resolve tissue- and species-level patterns in biomass composition throughout the year to enable harvest optimization based on TEA/LCA findings. Ultimately, elucidating the impact of biofeedstock dynamics on biorefinery performance enables harvest optimization, informed engineering design, and progress towards an expanded bioeconomy.

Shapiro, Alison J. [University of Delaware, Newark↗

In‐situ Analysis of Paste Properties in Resonant Acoustic Mixers for Quality Monitoring

Formulation control is key to achieving consistent target properties of energetic materials, as feedstock variations and slight deviations in the ratios of different ingredients can have major effects on final product properties, particularly in dense pastes with high particle loading >65 vol.%. In large‐scale operations, it is imperative to either correct or remove batches of material that perform outside baseline property specifications as early as possible to avoid unnecessary processing of suboptimal material. Quality monitoring is the practice of measuring material properties during processing using process analytical technologies as opposed to only testing the properties of the final product; it is a key principle in the quality‐by‐design frameworks used for designing formulations and manufacturing processes. Herein, a process analytical technology method for correlating material properties of dense pastes directly after mixing in a Resonant Acoustic Mixer to motor data is developed and used to detect differences in the particle content of dense paste formulations. This method was also capable of detecting variations in powder feedstock properties, such as particle packing efficiency, and is sensitive enough to detect changes of 2 wt.% in the total solids content of the formulation. The techniques presented herein show excellent promise for use as a process analytical technology capable of quantifying formulation effects on material movement modes during resonant acoustic mixing.

Materials science↗

Integrated thermal and biological conversion of microalgal proteins to lipids

Microalgal composition varies with cultivation strategy, and low-cost approaches often produce high-protein biomass. This presents challenges for biorefineries designed around static, lipid-rich feedstocks. In particular, hydrolysates from high-protein algae are nitrogen-rich and sugar-poor, limiting microbial conversion and reducing product yields. This study develops a sequential thermal conditioning and biological upgrading strategy to integrate high-protein hydrolysate processing within conventional lipid extraction and upgrading designs. Oxidative deconstruction was used to break down proteins into ammonium and short-chain carboxylates. Ammonium was subsequently removed to yield a nitrogen-depleted, carboxylate-rich medium suitable for microbial lipid production. Bioconversion trials with Cutaneotrichosporon oleaginosum showed lipid accumulation only from hydrolysates treated with both oxidative deconstruction and nitrogen removal, reaching 1.2 g/L lipids at 30 % intracellular content. This integrated approach enables protein-to-lipid conversion and improves flexibility to process variable algal feedstocks, advancing fuel-oriented microalgal biorefineries.

09 BIOMASS FUELS↗

Designing Cost‐Effective Carbon Payments to Induce Cellulosic Feedstock Production for Sustainable Aviation Fuel

Perennial bioenergy crops, such as miscanthus and switchgrass, and crop residues have the potential to scale up sustainable aviation fuel (SAF) production and mitigate carbon emissions. However, high establishment costs, delayed returns, and risk–return profiles that diverge from those of conventional crops can hinder incentives to adopt bioenergy crops. We develop an economic model that incorporates spatially varying joint yield and price distributions for the multiple crop choices a farmer faces and apply it to examine the incentives for risk-averse, present-biased, and credit-constrained farmers to produce cellulosic feedstocks under various biomass prices. We link this model to a biogeochemical model to quantify the spatially varying carbon mitigation benefits from these feedstocks in the rainfed region of the United States. We also analyze the cost-effectiveness of two carbon payment policies: annual and upfront. We find that risk-averse, present-biased, or credit-constrained farmers prefer to grow the lower-yielding but less risky switchgrass and harvest corn stover instead of the lower carbon, higher-yielding but riskier feedstock miscanthus, resulting in lower SAF production. Upfront carbon payments incentivize higher quantities of less carbon-intensive SAF production by risk-averse, credit-constrained, and present-biased farmers because they offset a part of the establishment costs of miscanthus. We also find that when farmers are credit-constrained, upfront payments are more cost-effective in terms of carbon mitigation per dollar spent. In contrast, annual payments are more cost-effective when farmers can access credit.

09 BIOMASS FUELS↗

Conversion of real-world aluminum scrap streams into high-performance Al–Mg–Si–Cu automotive alloys using shear assisted processing and extrusion

The conversion of post-consumer aluminum (Al) scrap into usable Al alloys without adding primary Al is challenging because of excess impurities. In this work, >99% post-consumer Twitch, used beverage cans (UBCs), and remelt scrap ingots (RSIs) were used as feedstock materials. As-cast and solution heat-treated feedstock billets were extruded using Shear Assisted Processing and Extrusion (ShAPE) at ~510°C, followed by press quenching. To explore the development of the microstructure, texture, and underlying mechanisms and how they contribute to the overall strengthening in as-extruded and artificially aged samples, scanning electron microscopy (SEM) and electron backscatter diffraction (EBSD) were used to collect microstructure and texture data. The enhanced strength and ductility were corroborated with the microstructural features and crystallographic texture. Simple shear $\textrm{A}/\bar{\textrm{A}}$, $\textrm{A}_1^*/\textrm{A} _2^*$ texture components along with weak $\textrm{C}$ and $\textrm{B}/\bar{\textrm{B}}$ texture components were formed during extrusion; the texture was strengthened after heat treatment. The refined second-phase particles helped to retain the deformed microstructure and texture. The contributions of dislocation and precipitate strengthening were maximized when billets were solution-heat-treated prior to extrusion. This is attributed to the formation of effective supersaturated solid solutions during the ShAPE process, which precipitate out during the peak age treatment. Overall, the highest yield strength of 305 MPa, ultimate tensile strength of 350 MPa, and elongation of 12% were achieved in artificially aged samples, which are comparable to those of Al 6082-T6.

Al alloy↗

Energy and nutrient recovery from municipal and industrial waste and wastewater—a perspective

This publication highlights the latest advancements in the field of energy and nutrient recovery from organics rich municipal and industrial waste and wastewater. Energy and carbon rich waste streams are multifaceted, including municipal solid waste, industrial waste, agricultural by-products and residues, beached or residual seaweed biomass from post-harvest processing, and food waste, and are valuable resources to overcome current limitations with sustainable feedstock supply chains for biorefining approaches. The emphasis will be on the most recent scientific progress in the area, including the development of new and innovative technologies, such as microbial processes and the role of biofilms for the degradation of organic pollutants in wastewater, as well as the production of biofuels and value-added products from organic waste and wastewater streams. The carboxylate platform, which employs microbiomes to produce mixed carboxylic acids through methane-arrested anaerobic digestion, is the focus as a new conversion technology. Nutrient recycling from conventional waste streams such as wastewater and digestate, and the energetic valorization of such streams will also be discussed. The selected technologies significantly contribute to advanced waste and wastewater treatment and support the recovery and utilization of carboxylic acids as the basis to produce many useful and valuable products, including food and feed preservatives, human and animal health supplements, solvents, plasticizers, lubricants, and even biofuels such as sustainable aviation fuel.

59 BASIC BIOLOGICAL SCIENCES↗

Development of vegetative oil sorghum: From lab‐to‐field

Summary Biomass crops engineered to accumulate energy‐dense triacylglycerols (TAG or ‘vegetable oils’) in their vegetative tissues have emerged as potential feedstocks to meet the growing demand for renewable diesel and sustainable aviation fuel (SAF). Unlike oil palm and oilseed crops, the current commercial sources of TAG, vegetative tissues, such as leaves and stems, only transiently accumulate TAG. In this report, we used grain (Texas430 or TX430) and sugar‐accumulating ‘sweet’ (Ramada) genotypes of sorghum, a high‐yielding, environmentally resilient biomass crop, to accumulate TAG in leaves and stems. We initially tested several gene combinations for a ‘push‐pull‐protect’ strategy. The top TAG‐yielding constructs contained five oil transgenes for a sorghum WRINKLED1 transcription factor (‘push’), a Cuphea viscosissima diacylglycerol acyltransferase (DGAT; ‘pull’), a modified sesame oleosin (‘protect’) and two combinations of specialized Cuphea lysophosphatidic acid acyltransferases and medium‐chain acyl‐acyl carrier protein thioesterases. Though intended to generate oils with medium‐chain fatty acids, engineered lines accumulated oleic acid‐rich oil to amounts of up to 2.5% DW in leaves and 2.0% DW in stems in the greenhouse, 36‐fold and 49‐fold increases relative to wild‐type (WT) plants, respectively. Under field conditions, the top‐performing event accumulated TAG to amount to 5.5% DW in leaves and 3.5% DW in stems, 78‐fold and 58‐fold increases, respectively, relative to WT TX430. Transcriptomic and fluxomic analyses revealed potential bottlenecks for increased TAG accumulation. Overall, our studies highlight the utility of a lab‐to‐field pipeline coupled with systems biology studies to deliver high vegetative oil sorghum for SAF and renewable diesel production.

59 BASIC BIOLOGICAL SCIENCES↗

Advanced pathways for hydrogen production: a collective view from a technical experts meeting

Hydrogen is an essential fuel and feedstock that can be produced in multiple ways to meet requirements for technological sectors that include energy storage, transportation, petroleum refining, and ammonia synthesis. To consider the future state of hydrogen manufacturing, a team of experts has assembled and examined three emerging hydrogen production technologies – photoelectrochemical, biological, and thermochemical. Each of these emerging technologies holds significant long-term potential for cost reduction while lowering industrial emissions associated with conventional methods of hydrogen manufacture (e.g., steam methane reforming) by using sunlight and renewable resources as primary sources of energy and feedstock, respectively. All three are currently at low technology readiness levels, however their applications, cost reduction opportunities and performance improvement pathways are under active development. In this work, opportunities and outlook for research that can directly advance the technologies are discussed.

08 HYDROGEN↗