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At least 235 records · Page 13

Global meta-analysis shows pervasive phosphorus limitation of aboveground plant production in natural terrestrial ecosystems

Phosphorus (P) limitation of aboveground plant production is usually assumed to occur in tropical regions but rarely elsewhere. Here we report that such P limitation is more widespread and much stronger than previously estimated. In our global meta-analysis, almost half (46.2%) of 652 P-addition field experiments reveal a significant P limitation on aboveground plant production. Globally, P additions increase aboveground plant production by 34.9% in natural terrestrial ecosystems, which is 7.0–15.9% higher than previously suggested. In croplands, by contrast, P additions increase aboveground plant production by only 13.9%, probably because of historical fertilizations. The magnitude of P limitation also differs among climate zones and regions, and is driven by climate, ecosystem properties, and fertilization regimes. In addition to confirming that P limitation is widespread in tropical regions, our study demonstrates that P limitation often occurs in other regions. This suggests that previous studies have underestimated the importance of altered P supply on aboveground plant production in natural terrestrial ecosystems.

Science & Technology - Other Topics↗

No carbon storage in growth-limited trees in a semi-arid woodland

Plant survival depends on a balance between carbon supply and demand. When carbon supply becomes limited, plants buffer demand by using stored carbohydrates (sugar and starch). During drought, NSCs (non-structural carbohydrates) may accumulate if growth stops before photosynthesis. This expectation is pervasive, yet few studies have combined simultaneous measurements of drought, photosynthesis, growth, and carbon storage to test this. Using a field experiment with mature trees in a semi-arid woodland, we show that growth and photosynthesis slow in parallel as ψ pd declines, preventing carbon storage in two species of conifer (J. monosperma and P. edulis). During experimental drought, growth and photosynthesis were frequently co-limited. Our results point to an alternative perspective on how plants use carbon that views growth and photosynthesis as independent processes both regulated by water availability.

54 ENVIRONMENTAL SCIENCES↗

Oxygen priming induced by elevated CO 2 reduces carbon accumulation and methane emissions in coastal wetlands

Warming temperatures and elevated carbon dioxide (CO 2 ) are inextricably linked global change phenomena, but are rarely manipulated together in field experiments. As a result, ecosystem-level responses to these interacting facets of global change remain poorly understood. In 2017, we started an experiment in a coastal wetland that crosses warming and elevated CO 2 , to better understand how the interactions of these factors affect the plants, biogeochemistry, and overall survival of these ecosystems. However, contrary to our expectations, when elevated CO 2 is combined with warming, the wetland accumulated less carbon, making it more vulnerable to sea-level rise. This was due to the plants transporting more oxygen into the soil, which stimulated aerobic decomposition. At the same time, the increased supply of oxygen reduced net methane (CH 4 ) emissions from the system, even though warming alone substantially increased CH 4 emission. Together, these results demonstrate that ecosystem responses to interacting facets of global change may be mediated by plant traits that regulate the soil environment. Thus, plant responses are critical for predicting future ecosystem survival and climate feedbacks.

54 ENVIRONMENTAL SCIENCES↗

Depth-dependent Metagenome-Assembled Genomes of Agricultural Soils under Managed Aquifer Recharge

Abstract Managed Aquifer Recharge (MAR) systems, which intentionally replenish groundwater aquifers with excess water, are critical for addressing water scarcity exacerbated by demographic shifts and climate variability. To date, little is known about the functional diversity of the soil microbiome at different soil depth inhabiting agricultural soils used for MAR. Knowing the functional diversity is pivotal in regulating nutrient cycling and maintaining soil health. Metagenomics, particularly Metagenome-Assembled Genomes (MAGs), provide a powerful tool to explore the diversity of uncultivated soil microbes, facilitating in-depth investigations into microbial functions. In a field experiment conducted in a California vineyard, we sequenced soil DNA before and after water application of MAR. Through this process, we assembled 146 medium and 14 high-quality MAGs, uncovering a wide array of archaeal and bacterial taxa across different soil depths. These findings advance our understanding of the microbial ecology and functional diversity of soils used for MAR, contributing to the development of more informed and sustainable land management strategies.

Science & Technology - Other Topics↗

The Q 10 of in situ microbial soil respiration varies with mean annual temperature, precipitation, pH, and plant cover: a meta-analysis and spatial prediction of Q 10

The temperature sensitivity of soil microbial respiration, commonly quantified using the Q 10 coefficient, is a key parameter in carbon cycle models. Uncovering how environmental factors affect in situ Q 10 values can therefore provide critical insight into potential shifts in global carbon stocks under climate change. We collected data from previously published field experiments that measured soil microbial respiration across a range of temperatures. We hypothesized that the Q 10 coefficient of in situ soil microbial respiration would vary based on environmental factors including mean annual temperature (MAT), mean annual precipitation (MAP), plant cover type, pH, soil C:N, and latitude. Linear regression revealed that Q 10 correlates negatively with MAT and MAP and positively with pH and absolute latitude. Additionally, average Q 10 varied significantly across different plant cover types; it was highest in mountain grasslands and lowest in tropical moist forests. Variation in microbial Q 10 across environmental factors may arise from underlying mechanisms such as enzyme kinetics, substrate availability and complexity, and microbial adaptation. To capture patterns in Q 10 more comprehensively, we developed a multiple linear regression model of Q 10 based on the most individually significant environmental drivers and applied it to public datasets to generate a global map of predicted Q 10 . Q 10 was higher in high-latitude and high-altitude regions, where large permafrost carbon stores are vulnerable to thawing and decomposition. We also compared fits between the Q 10 equation and a model produced from macromolecular rate theory (MMRT). We found that the MMRT model had the superior fit and may be better suited to model temperature sensitivity of complex biological reactions. Overall, our results emphasize that relationships between microbial Q 10 and environmental variables should be accounted for in climate models. Incorporating these variations in the Q 10 parameter, rather than using a fixed value, will help predict whether CO 2 emissions will be buffered or exacerbated by soil microbial respiration under climate change.

54 ENVIRONMENTAL SCIENCES↗

Evidence and attribution of the enhanced land carbon sink

Climate change has been partially mitigated by an increasing net land carbon sink in the terrestrial biosphere; understanding the processes that drive this sink is thus essential for protecting, managing and projecting this important ecosystem service. In this Review, we examine evidence for an enhanced land carbon sink and attribute the observed response to drivers and processes. This sink has doubled from 1.2 ± 0.5 PgC yr -1 in the 1960s to 3.1 ± 0.6 PgC yr -1 in the 2010s. This trend results largely from carbon dioxide fertilization increasing photosynthesis (driving an increase in the annual land carbon sink of >2 PgC globally since 1900), mainly in tropical forest regions, and elevated temperatures reducing cold limitation, mainly at higher latitudes. Continued long-term land carbon sequestration is possible through the end of this century under multiple emissions scenarios, especially if nature-based climate solutions and appropriate ecosystem management are used. In conclusion, a new generation of globally distributed field experiments is needed to improve understanding of future carbon sink potential by measuring belowground carbon release, the response to carbon dioxide enrichment, and long-term shifts in carbon allocation and turnover.

54 ENVIRONMENTAL SCIENCES↗

Coastal groundwater salinization impairs tree carbon–water balance

How coastal forest productivity varies with local nutrient availability and water supply remains a knowledge gap under climate change. In a two-decade field experiment manipulating fertilization and density in coastal pine forests, we show that a decade of growth enhancement by simulated sedimentary nutrient inputs has resulted in a striking reversal in growth and increased mortality risk as drought and sea level rise progressed. Recent groundwater salinization has further triggered a shift from nutrient to water limitation, causing severe stomatal closure and decoupling of tree carbon–water balance, which induces a negative intrinsic water use efficiency (iWUE)–growth relationship. Higher tree iWUE predicted sharper tree growth declines, and both nutrient enrichment and high stand density amplified this feedback, increasing the risk of hydraulic failure and mortality. These results suggest that a transient nutrient-stimulatory effect could drive further coastal forest degradation due to heightened belowground saltwater stress under sea-level rise.

Climate-change ecology↗

Model fusion with physics-guided machine learning: Projection-based reduced-order modeling

The unprecedented amount of data generated from experiments, field observations, and large-scale numerical simulations at a wide range of spatiotemporal scales has enabled the rapid advancement of data-driven and especially deep learning models in the field of fluid mechanics. Although these methods are proven successful for many applications, there is a grand challenge of improving their generalizability. This is particularly essential when data-driven models are employed within outer-loop applications like optimization. In this work, we put forth a physics-guided machine learning (PGML) framework that leverages the interpretable physics-based model with a deep learning model. Leveraging a concatenated neural network design from multi-modal data sources, the PGML framework is capable of enhancing the generalizability of data-driven models and effectively protects against or inform about the inaccurate predictions resulting from extrapolation. We apply the PGML framework as a novel model fusion approach combining the physics-based Galerkin projection model and long- to short-term memory (LSTM) network for parametric model order reduction of fluid flows. We demonstrate the improved generalizability of the PGML framework against a purely data-driven approach through the injection of physics features into intermediate LSTM layers. Our quantitative analysis shows that the overall model uncertainty can be reduced through the PGML approach, especially for test data coming from a distribution different than the training data. Moreover, we demonstrate that our approach can be used as an inverse diagnostic tool providing a confidence score associated with models and observations. The proposed framework also allows for multi-fidelity computing by making use of low-fidelity models in the online deployment of quantified data-driven models.

42 ENGINEERING↗

Natural selection maintains species despite frequent hybridization in the desert shrub Encelia

Natural selection is an important driver of genetic and phenotypic differentiation between species. For species in which potential gene flow is high but realized gene flow is low, adaptation via natural selection may be a particularly important force maintaining species. For a recent radiation of New World desert shrubs ( Encelia : Asteraceae), we use fine-scale geographic sampling and population genomics to determine patterns of gene flow across two hybrid zones formed between two independent pairs of species with parapatric distributions. After finding evidence for extremely strong selection at both hybrid zones, we use a combination of field experiments, high-resolution imaging, and physiological measurements to determine the ecological basis for selection at one of the hybrid zones. Our results identify multiple ecological mechanisms of selection (drought, salinity, herbivory, and burial) that together are sufficient to maintain species boundaries despite high rates of hybridization. Given that multiple pairs of Encelia species hybridize at ecologically divergent parapatric boundaries, such mechanisms may maintain species boundaries throughout Encelia .

59 BASIC BIOLOGICAL SCIENCES↗

Adaptive differentiation and rapid evolution of a soil bacterium along a climate gradient

Significance Increasing evidence suggests that evolutionary processes frequently shape ecological patterns; however, most microbiome studies thus far have focused on only the ecological responses of these communities. By using parallel field experiments and focusing in on a model soil bacterium, we showed that bacterial “species” are differentially adapted to local climates, leading to changes in their composition. Furthermore, we detected strain-level evolution, providing direct evidence that both ecological and evolutionary processes operate on annual timescales. The consideration of eco-evolutionary dynamics may therefore be important to understand the response of soil microbiomes to future environmental change.

54 ENVIRONMENTAL SCIENCES↗

Climate change–induced stress disrupts ectomycorrhizal interaction networks at the boreal–temperate ecotone

The interaction networks formed by ectomycorrhizal fungi (EMF) and their tree hosts, which are important to both forest recruitment and ecosystem carbon and nutrient retention, may be particularly susceptible to climate change at the boreal–temperate forest ecotone where environmental conditions are changing rapidly. Here, we quantified the compositional and functional trait responses of EMF communities and their interaction networks with two boreal ( Pinus banksiana and Betula papyrifera ) and two temperate ( Pinus strobus and Quercus macrocarpa ) hosts to a factorial combination of experimentally elevated temperatures and reduced rainfall in a long-term open-air field experiment. The study was conducted at the B4WarmED (Boreal Forest Warming at an Ecotone in Danger) experiment in Minnesota, USA, where infrared lamps and buried heating cables elevate temperatures (ambient, +3.1 °C) and rain-out shelters reduce growing season precipitation (ambient, ~30% reduction). EMF communities were characterized and interaction networks inferred from metabarcoding of fungal-colonized root tips. Warming and rainfall reduction significantly altered EMF community composition, leading to an increase in the relative abundance of EMF with contact-short distance exploration types. These compositional changes, which likely limited the capacity for mycelial connections between trees, corresponded with shifts from highly redundant EMF interaction networks under ambient conditions to less redundant (more specialized) networks. Further, the observed changes in EMF communities and interaction networks were correlated with changes in soil moisture and host photosynthesis. Collectively, these results indicate that the projected changes in climate will likely lead to significant shifts in the traits, structure, and integrity of EMF communities as well as their interaction networks in forest ecosystems at the boreal–temperate ecotone.

54 ENVIRONMENTAL SCIENCES↗

Lifetime fatigue response due to wake steering on a pair of utility-scale wind turbines

Quantifying the impacts on turbine loads during wind farm control is an important consideration in assessing power production benefits. Wake steering controls aim to improve total wind farm performance by coordinating the control actions of individual turbines, wherein an upstream turbine is intentionally yawed at an offset angle from the measured wind direction. Consequently, this redirects its wake for improved power production and potentially reduces fatigue loads of the downstream turbines. This paper studies the lifetime fatigue loads associated with wake steering by using utility-scale wind turbine experimental data to conduct an analysis on a pair of wind turbines. This study was part of a large field experiment in which a group of five GE 1.5-MW SLE CWE turbines were selected as targets for conducting wake steering research. A standard loads instrumentation package and data acquisition system were installed on two turbines within the cluster to measure turbine fundamental loads. The time-series databases were used to calculate loads statistics as well as short-term and lifetime damage equivalent loads. Fatigue calculations followed the guidance in Annex H of the International Electrotechnical Commission standard 61400-1, Edition 4. Lifetime fatigue calculation results are presented in this analysis; three methods of assessing lifetime fatigue were used to determine percent difference for blade root moments, main shaft moments, main shaft torque, and tower top torque. For all three fatigue treatments, some components’ lifetime fatigue increases for the controlled turbine; however, the downwind turbine experienced a reduction in lifetime fatigue and combined effect for the turbine pair results in a reduction of fatigue when wake steering controls are applied.

17 WIND ENERGY↗

The case for digital twins in metal additive manufacturing

The digital twin (DT) is a relatively new concept that is finding increased acceptance in industry. A DT is generally considered as comprising a physical entity, its virtual replica, and two-way digital data communications in-between. Its primary purpose is to leverage the process intelligence captured within digital models—or usually their faster-solving surrogates—towards generating increased value from the physical entities. The surrogate models are created using machine learning based on data obtained from the field, experiments and digital models, which may be physics-based or statistics-based. Anomaly detection and correction, and diagnostic closed-loop process control are examples of how a process DT can be deployed. In the manufacturing industry, its use can achieve improvements in product quality and process productivity. Metal additive manufacturing (AM) stands to gain tremendously from the use of DTs. This is because the AM process is inherently chaotic, resulting in poor repeatability. However, a DT acting in a supervisory role can inject certainty into the process by actively keeping it within bounds through real-time control commands. Closed-loop feedforward control is achieved by observing the process through sensors that monitor critical parameters and, if there are any deviations from their respective optimal ranges, suitable corrective actions are triggered. The type of corrective action (e.g. a change in laser power or a modification to the scanning speed) and its magnitude are determined by interrogating the surrogate models. Because of their artificial intelligence (AI)-endowed predictive capabilities, which allow them to foresee a future state of the physical twin (e.g. the AM process), DTs proactively take context-sensitive preventative steps, whereas traditional closed-loop feedback control is usually reactive. Apart from assisting a build process in real-time, a DT can help with planning the build of a part by pinpointing the optimum processing window relevant to the desired outcome. Again, the surrogate models are consulted to obtain the required information. In this article, we explain how the application of DTs to the metal AM process can significantly widen its application space by making the process more repeatable (through quality assurance) and cheaper (by getting builds right the first time).

36 MATERIALS SCIENCE↗

Tracking fungal species-level responses in soil environments exposed to long-term warming and associated drying

Climate change is affecting fungal communities and their function in terrestrial ecosystems. Despite making progress in the understanding of how the fungal community responds to global change drivers in natural ecosystems, little is known on how fungi respond at the species level. Understanding how fungal species respond to global change drivers, such as warming, is critical, as it could reveal adaptation pathways to help us to better understand ecosystem functioning in response to global change. Here, we present a model study to track species-level responses of fungi to warming—and associated drying—in a decade-long global change field experiment; we focused on two free-living saprotrophic fungi which were found in high abundance in our site, Mortierella and Penicillium. Using microbiological isolation techniques, combined with whole genome sequencing of fungal isolates, and community level metatranscriptomics, we investigated transcription-level differences of functional categories and specific genes involved in catabolic processes, cell homeostasis, cell morphogenesis, DNA regulation and organization, and protein biosynthesis. We found that transcription-level responses were mostly species-specific but that under warming, both fungi consistently invested in the transcription of critical genes involved in catabolic processes, cell morphogenesis, and protein biosynthesis, likely allowing them to withstand a decade of chronic stress. Overall, our work supports the idea that fungi that invest in maintaining their catabolic rates and processes while growing and protecting their cells may survive under global climate change.

59 BASIC BIOLOGICAL SCIENCES↗

Towards the usage of quarry blasts as an active seismic source for subsurface characterization and imaging

SUMMARY Active or man-made seismic sources are often used to delineate subsurface geological structures via seismic imaging techniques. Although conventional controlled seismic sources have provided high-resolution subsurface images, the high cost of data acquisition necessitates improved use of cheaper alternative seismic sources for subsurface imaging, such as traffic noise, quarry blasts and earthquakes. Here, we explore the potential use of quarry blasts as an active seismic source for subsurface imaging. The goal of our study is to demonstrate the utility of quarry blasts as seismic sources for investigating subsurface structure with a field experiment in Mill Creek, southern Oklahoma. We deployed a 7455-m-long receiver line of 72 receivers (105 m spacing) extending away from a quarry, with a recording time of 28 d. We analyse similarities between blasts, wave types, origin time and excitation location of quarry blasts, which is important information for using such signal as an active source. Given a relative homogeneous geological condition in the study area, we could not observe clear reflected waves. However, the use of P- and S-wave velocities led to the delineation of a major basement damage zone in the study area, which further demonstrates the viability of quarry blasts as a useful seismic source for subsurface geological imaging.

58 GEOSCIENCES↗

Offline Arterial Signal Timing Optimization for Closely Spaced Intersections

The stop-and-go driving behavior at a busy arterial causes a significant amount of fuel waste and emissions that could be reduced. For closely spaced intersections, the traditional bottom-up signal timing approach could fail under high volumes when the queues reach the upstream intersections. A well-coordinated arterial should manage the queue length to prevent the control from failing. Traditionally, coordination among signalized intersections for an arterial means finding the good offsets for each intersection. The control of the intersections along an arterial can also be coordinated by optimizing splits. Splits impact the capacity of an intersection. Similar to the highway, the capacity drop could cause growing queues. This work is based on the Virtual Phase-Link (VPL) model, a street traffic model designed for online traffic model predictive control, to obtain a top-down offline arterial signal timing. The VPL-based model can guarantee capacity consistency in intersections along an arterial. We, therefore, adopted the VPL-based model and developed an offline signal timing optimization approach. The proposed timing derived from the VPL-based offline signal timing optimization showed very good results in simulation. We also collected field experiment data, which demonstrated overall energy reductions and speed improvements on some sections of the study arterial.

47 OTHER INSTRUMENTATION↗

Measurement of DC Arc-Flash Incident Energy in Large-Scale Photovoltaic Plants: A Basis for Standardization

The deployment of high-power dc equipment is increasing in solar photovoltaic (PV) plants, but very few studies have quantified dc arc-flash risks. Currently, PV plant owners and operators rely on theoretical, simplified models, such as those in NFPA-70E and other publications, for the assessment of risk associated with dc arc-flash. Here, this article presents an overview of arc-flash risks in a PV system based on a series of field experiments based on IEEE-1584 in two large-scale ground-mounted PV plants. The experiments include various high-power dc equipment of a PV plant, such as central inverters, combiner boxes, recombiner boxes, string inverters, and multiple configurations of electrodes in a 20-in calibration cube. The study reveals that none of the available dc arc-flash models are applicable for a PV plant. This work is an important first step toward developing an improved model that more accurately assesses dc arc-flash risk in a PV plant.

14 SOLAR ENERGY↗

Controlling Air Conditioners for Frequency Regulation: A Real-World Example

Even though thermostatically controlled loads like air conditioners present a great potential for providing ancillary services to the electric power grid, the practical challenges associated with their real-time coordination have not received the necessary attention. In this work, we present a nondisruptive load control application, specifically, we demonstrate how real residential air conditioners can provide frequency regulation. Aggregate power adjustment is achieved by modifying the ON/OFF modes of the air conditioners. To account for both single and multi-zone houses, we extend the currently available techniques and develop an approach that can be used for controlling aggregations that include both types of houses. A discussion of the practical challenges encountered in our field experiments is provided, along with the hardware and software approaches we developed to circumvent them. Here, we argue that limitations of current thermostat APIs introduce significant challenges and are an impediment to widespread adoption of fast load control applications.

24 POWER TRANSMISSION AND DISTRIBUTION↗