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At least 235 records · Page 13

All sources NASA NTRS DOE OSTI

DOE OSTI2023

Manipulating the 3D organization of the largest synthetic yeast chromosome

Whether synthetic genomes can power life has attracted broad interest in the synthetic biology field. Here, we report de novo synthesis of the largest eukaryotic chromosome thus far, synIV, a 1,454,621-bp yeast chromosome resulting from extensive genome streamlining and modification. We developed megachunk assembly combined with a hierarchical integration strategy, which significantly increased the accuracy and flexibility of synthetic chromosome construction. Besides the drastic sequence changes, we further manipulated the 3D structure of synIV to explore spatial gene regulation. Surprisingly, we found few gene expression changes, suggesting that positioning inside the yeast nucleoplasm plays a minor role in gene regulation. Lastly, we tethered synIV to the inner nuclear membrane via its hundreds of loxPsym sites and observed transcriptional repression of the entire chromosome, demonstrating chromosome-wide transcription manipulation without changing the DNA sequences. Our manipulation of the spatial structure of synIV sheds light on higher-order architectural design of the synthetic genomes.

59 BASIC BIOLOGICAL SCIENCES↗

NASA NTRS2003

Liquid Propellant Manipulated Acoustically

Fluids are difficult to manage in the space environment. Without gravity, the liquid and gas do not always remain separated as they do in the 1g environment of Earth. Instead the liquid and gas volumes mix and migrate under the influence of surface tension, thermodynamic forces, and external disturbances. As a result, liquid propellants may not be in a useable location or may even form a chaotic mix of liquid and gas bubbles. In the past, mechanical pumps, baffles, and a variety of specialized passive devices have been used to control the liquid and gas volumes. These methods need to be carefully tuned to a specific configuration to be effective. With increasing emphasis on long-term human activity in space there is a trend toward liquid systems that are more flexible and provide greater control. We are exploring new methods of manipulating liquids by using the nonlinear acoustic effects achieved by using beams of highly directed high-intensity acoustic waves.

Oeftering, Richard C.↗

NASA NTRS1996

Ergonomic Evaluations of Microgravity Workstations

Various gloveboxes (GBXs) have been used aboard the Shuttle and ISS. Though the overall technical specifications are similar, each GBX's crew interface is unique. JSC conducted a series of ergonomic evaluations of the various glovebox designs to identify human factors requirements for new designs to provide operator commonality across different designs. We conducted 2 0g evaluations aboard the Shuttle to evaluate the material sciences GBX and the General Purpose Workstation (GPWS), and a KC-135 evaluation to compare combinations of arm hole interfaces and foot restraints (flexible arm holes were better than rigid ports for repetitive fine manipulation tasks). Posture analysis revealed that the smallest and tallest subjects assumed similar postures at all four configurations, suggesting that problematic postures are not necessarily a function of the operator s height but a function of the task characteristics. There was concern that the subjects were using the restrictive nature of the GBX s cuffs as an upper-body restraint to achieve such high forces, which might lead to neck/shoulder discomfort. EMG data revealed more consistent muscle performance at the GBX; the variability in the EMG profiles observed at the GPWS was attributed to the subjects attempts to provide more stabilization for themselves in the loose, flexible gauntlets. Tests revealed that the GBX should be designed for a 95 percentile American male to accommodate a neutral working posture. In addition, the foot restraint with knee support appeared beneficial for GBX operations. Crew comments were to provide 2 foot restraint mechanical modes, loose and lock-down, to accommodate a wide range of tasks without egressing the restraint system. Thus far, we have developed preliminary design guidelines for GBXs and foot.

Whitmore, Mihriban↗

DOE OSTI2023

Size-Dependent Thermal Shifts to MOF Nanocrystal Optical Gaps Induced by Dynamic Bonding

Conventional semiconductor nanocrystals exhibit wide-ranging optical behavior, whereas the size-dependent photophysical properties of metal–organic framework (MOF) nanocrystals remain an open research frontier. Furthermore, we present size- and temperature-dependent optical absorption spectra of common MOFs with particle sizes ranging from tens of nanometers to several micrometers. All materials exhibit optical gaps that decrease at elevated temperatures, which we attribute to the dynamic nature of MOF metal–linker bonds. Accordingly, whereas the labile titanium–carboxylate bonds of MIL-125 give rise to bandgaps that red-shift by ~600 meV over 300 K, the more rigid zinc–imidazolate bonds of ZIF-8 produce a red-shift of only ~10 meV. Furthermore, smaller particles induce far larger decreases to optical gaps. Taken together, these results suggest MOF bonding becomes more flexible with smaller nanocrystal sizes, offering a powerful tool for manipulating optical behavior through composition, temperature, and dimensionality.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗

DOE OSTI2022

A New Integrated Analysis Suite for Fast-Ion Study in KSTAR

Here, an integrated workflow for fast-ion analysis was developed by adapting the One Modeling Framework for Integrated Task (OMFIT) workflow manager to support a standard and unified analysis platform for KSTAR users. The newly established analysis suite offers a graphical user interface–based workflow to enable users to readily access and handle experimental data archived in various data formats and servers. Further, users can analyze the data by importing modules designed for conducting certain tasks, such as profile fitting, equilibrium reconstruction, and postprocessing of tokamak data. The procedures for preparing the inputs for fast-ion simulations are streamlined by a common workflow manager, which enables the parallel processing of various tasks to efficiently analyze large fast-ion datasets. The OMFIT platform comprises a flexible Python-based application that enables users to freely manipulate the Python scripts for applications that are unavailable in the standard workflow. The framework also offers mapping tools to translate the output data into the Integrated Modeling and Analysis Suite format to maintain application compatibility for future ITER burning plasma experiments.

70 PLASMA PHYSICS AND FUSION TECHNOLOGY↗

DOE OSTI2025

Pseudogap and Fermi arc induced by Fermi surface nesting in a centrosymmetric skyrmion magnet

Skyrmions in noncentrosymmetric materials are believed to occur due to the Dzyaloshinskii-Moriya interaction. By contrast, the skyrmion formation mechanism in centrosymmetric materials remains elusive. Here, in this work, we reveal the intrinsic electronic structure of the centrosymmetric GdRu 2 Si 2 by selectively measuring magnetic domains using angle-resolved photoemission spectroscopy (ARPES). We found robust Fermi surface (FS) nesting, consistent with the magnetic modulation q -vector detected by the previous resonant x-ray scattering measurements. The pseudogap opens at the nested FS portions, which vary for different magnetic domains. The anomalous pseudogap disconnects the FS to generate Fermi arcs with twofold symmetry. These results indicate that the Ruderman-Kittel-Kasuya-Yosida (RKKY) interaction plays a decisive role in generating the screw spin modulation responsible for the skyrmion formation in GdRu 2 Si 2 . Furthermore, we demonstrate the flexible nature of magnetism in GdRu 2 Si 2 by manipulating magnetic domains with magnetic field and temperature cyclings, providing potential future applications for data storage and processing devices.

71 CLASSICAL AND QUANTUM MECHANICS, GENERAL PHYSIC↗

NASA NTRS1987

Implementation of a robotic flexible assembly system

As part of the Intelligent Task Automation program, a team developed enabling technologies for programmable, sensory controlled manipulation in unstructured environments. These technologies include 2-D/3-D vision sensing and understanding, force sensing and high speed force control, 2.5-D vision alignment and control, and multiple processor architectures. The subsequent design of a flexible, programmable, sensor controlled robotic assembly system for small electromechanical devices is described using these technologies and ongoing implementation and integration efforts. Using vision, the system picks parts dumped randomly in a tray. Using vision and force control, it performs high speed part mating, in-process monitoring/verification of expected results and autonomous recovery from some errors. It is programmed off line with semiautomatic action planning.

Benton, Ronald C.↗

NASA NTRS1994

Copyright Ownership in a Networked Multimedia Environment

The explosion of computer communications in the United States has spurred the development of many new technologies. One of these new technologies is Mosaic and the World-Wide Web. Mosaic is a user interface that uses the internet as a backbone for communications. The Mosaic interface enables a user to manipulate text, images and graphics produced by different authors. The flexibility that Mosaic offers raises significant copyright issues. This paper attempts to analyze these issues using current copyright law as a framework. The author then goes on to offer a different analysis that may result from future developments in copyright law.

Williams, Vernon E.↗

NASA NTRS2014

Design and Control of Modular Spine-Like Tensegrity Structures

We present a methodology enabled by the NASA Tensegrity Robotics Toolkit (NTRT) for the rapid structural design of tensegrity robots in simulation and an approach for developing control systems using central pattern generators, local impedance controllers, and parameter optimization techniques to determine effective locomotion strategies for the robot. Biomimetic tensegrity structures provide advantageous properties to robotic locomotion and manipulation tasks, such as their adaptability and force distribution properties, flexibility, energy efficiency, and access to extreme terrains. While strides have been made in designing insightful static biotensegrity structures, gaining a clear understanding of how a particular structure can efficiently move has been an open problem. The tools in the NTRT enable the rapid exploration of the dynamics of a given morphology, and the links between structure, controllability, and resulting gait efficiency. To highlight the effectiveness of the NTRT at this exploration of morphology and control, we will provide examples from the designs and locomotion of four different modular spine-like tensegrity robots.

Modular↗

NASA NTRS1987

IECON '87: Industrial applications of control and simulation; Proceedings of the 1987 International Conference on Industrial Electronics, Control, and Instrumentation, Cambridge, MA, Nov. 3, 4, 1987

Recent advances in control-system design and simulation are discussed in reviews and reports. Among the topics considered are fast algorithms for generating near-optimal binary decision programs, trajectory control of robot manipulators with compensation of load effects via a six-axis force sensor, matrix integrators for real-time simulation, a high-level control language for an autonomous land vehicle, and a practical engineering design method for stable model-reference adaptive systems. Also addressed are the identification and control of flexible-limb robots with unknown loads, adaptive control and robust adaptive control for manipulators with feedforward compensation, adaptive pole-placement controllers with predictive action, variable-structure strategies for motion control, and digital signal-processor-based variable-structure controls.

Hartley, Tom T.↗

NASA NTRS2000

Automated Multiple-Sample Tray Manipulation Designed and Fabricated for Atomic Oxygen Facility

Extensive improvements to increase testing capacity and flexibility and to automate the in situ Reflectance Measurement System (RMS) are in progress at the Electro-Physics Branch s Atomic Oxygen (AO) beam facility of the NASA Glenn Research Center at Lewis Field. These improvements will triple the system s capacity while placing a significant portion of the testing cycle under computer control for added reliability, repeatability, and ease of use.

Sechkar, Edward A.↗

NASA NTRS1980

An investigation of dynamic-analysis methods for variable-geometry structures

Selected space structure configurations were reviewed in order to define dynamic analysis problems associated with variable geometry. The dynamics of a beam being constructed from a flexible base and the relocation of the completed beam by rotating the remote manipulator system about the shoulder joint were selected. Equations of motion were formulated in physical coordinates for both of these problems, and FORTRAN programs were developed to generate solutions by numerically integrating the equations. These solutions served as a standard of comparison to gauge the accuracy of approximate solution techniques that were developed and studied. Good control was achieved in both problems. Unstable control system coupling with the system flexibility did not occur. An approximate method was developed for each problem to enable the analyst to investigate variable geometry effects during a short time span using standard fixed geometry programs such as NASTRAN. The average angle and average length techniques are discussed.

Austin, F.↗

NASA NTRS1979

Space transportation system options for extended duration and power

A modification kit for the Space Transportation System (STS) Orbiter is proposed to provide more power and mission duration for payloads. The power extension package (PEP) - a flexible-substrate solar array deployed on the Space Shuttle Orbiter remote manipulator system - can provide as much as 29 kW total power for durations of 10 to 48 days. The kit is installed only for those flights which require enhanced power or duration. Modifications to the Orbiter thermal control and life support systems to improve heat balance and to reduce consumables are proposed. The changes consist of repositioning the Orbiter forward radiators and replacing the lithium hydroxide scrubber with a regenerable solid amine.

Loftus, J. P., Jr.↗

NASA NTRS1986

An apparatus for protein crystal growth studies

An apparatus is described for observing protein crystals growing under varying growth conditions, and for changing or manipulating crystal orientation for viewing selected faces or growth modes. This flexibility permits a wide variety of protein crystal growth experiments to be performed using the same crystal(s).

Pusey, Marc L.↗

NASA NTRS1991

Manipulation strategies for massive space payloads

The industrial and environmental applications for robots with a relatively large workspace has increased significantly in the last few years. To accommodate the demands, the manipulator is usually designed with long, lightweight links that are inherently flexible. Ongoing research at Georgia Tech into the behavior and design of these flexible links is discussed.

Book, Wayne J.↗

NASA NTRS2013

Polymer-Reinforced, Non-Brittle, Lightweight Cryogenic Insulation

The primary application for cryogenic insulating foams will be fuel tank applications for fueling systems. It is crucial for this insulation to be incorporated into systems that survive vacuum and terrestrial environments. It is hypothesized that by forming an open-cell silica-reinforced polymer structure, the foam structures will exhibit the necessary strength to maintain shape. This will, in turn, maintain the insulating capabilities of the foam insulation. Besides mechanical stability in the form of crush resistance, it is important for these insulating materials to exhibit water penetration resistance. Hydrocarbon-terminated foam surfaces were implemented to impart hydrophobic functionality that apparently limits moisture penetration through the foam. During the freezing process, water accumulates on the surfaces of the foams. However, when hydrocarbon-terminated surfaces are present, water apparently beads and forms crystals, leading to less apparent accumulation. The object of this work is to develop inexpensive structural cryogenic insulation foam that has increased impact resistance for launch and ground-based cryogenic systems. Two parallel approaches will be pursued: a silica-polymer co-foaming technique and a post foam coating technique. Insulation characteristics, flexibility, and water uptake can be fine-tuned through the manipulation of the polyurethane foam scaffold. Silicate coatings for polyurethane foams and aerogel-impregnated polyurethane foams have been developed and tested. A highly porous aerogel-like material may be fabricated using a co-foam and coated foam techniques, and can insulate at liquid temperatures using the composite foam

Hess, David M.↗

NASA NTRSDate not supplied

TPSAS-NF1676L-21010-DND

The primary objective of this work is the dynamic analyses of the Tendon-Actuated Lightweight In-Space Manipulator (TALISMAN) which entails structural dynamics, multi-body dynamics and flexible multi-body dynamics modeling and simulation of robotic systems, associated space vehicles and operations. The dynamic analysis is in the critical path of TALISMAN technology development for utilization in the Asteroid Redirect Mission (ARM) and for potential application to Satellite Servicing Missions (SSM). In addition, modeling work supports control design, and the testing of space operations.

Cornelia Altenbuchner↗

NASA NTRS1987

Solid modelling for the manipulative robot arm (power) and adaptive vision control for space station missions

The structure of a flexible arm derived from concatenation of the Stewart-Table-based links were studied. Solid modeling provides not only a realistic simulation, but is also essential for studying vision algorithms. These algorithms could be used for the adaptive control of the arm, using the well-known algorithms such as shape from shading, edge detection, orientation, etc. Details of solid modeling and its relation to vision based adaptive control are discussed.

Harrand, V.↗