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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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At least 235 records · Page 13
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NASA NTRS1994

The high-state/low-state transition in V794 Aquilae

We present a V-magnitude light curve of the cataclysmic binary V794 Quilae covering an 800 day time span. The system shows variations of ups to 3 mag. In particular, there are two dips of approximately 1 mag which last for about 50 days, and are followed by a rapid recovery to the original brightness, and a third dip of approximately 3 mag lasting about 100 days. These fluctuations are thought to be caused by the response of the accretion disk in the system to the cessation of mass transfer from the mass-losing secondary star. We present computations using a time-dependent accretion disk code to delineate a range of allowed behavior for th accretion disk. To model the observed light curve, we require that the model parameters alpha(sub cold) and alpha(sub hot), which characterize the degree of coupling of the viscous dissipation to the orbital shear in the low and high states of the accretion disk, be smaller than they have been inferred to be in the dwarf novae, and that the ratio alpha(sub hot)/alpha(sub cold) also be smaller. The fact that alpha seems to vary with the rate of mass transfer may provide support for the model of Vishniac and Diamond, in which the impact of the mass transfer stream onto the outer edge of the accretion disk excites internal waves which transport angular momentum outward and provide the viscous dissipation.

Honeycutt, R. Kent↗
NASA NTRS1994

HST images of nearby luminous quasars

Strong upper limits are placed on the visual-band brightness of galactic hosts for four luminous, radioquiet quasars with redshifts between 0.16 and 0.24 that were studied with the Hubble Space Telescope (HST's) Wide Field/Planetary Camera 2 (WFPC2). Typical upper limits on the luminosities of galactic hosts are approximately 1.4 mag fainter than L* for spirals and approximately 0.5 mag fainter than L* for ellipticals. The galactic hosts of the quasars are more than a magnitude and a half fainter than the median integrated absolute magnitude of Seyfert galaxies. If the detection limits are determined using featureless simulated galaxies instead of observed galaxy images, then the detection limits for spirals are 0.5-1.0 mag less stringent. These results are consistent with the hypothesis that the quasar phenomenon corresponds to the early stages of galaxy formation, before extensive star formation occurs.

Bahcall, John N.↗
NASA NTRS1994

WFPC2 observations of the globular cluster M30

We describe images of the center of the dense globular cluster M30 (NGC 7099) obtained with the Hubble Space Telescope Wide Field/Planetary Camera 2 (HST WFPC2). Data taken in the F336W, F439W, and F555W filters (approximately U, B, and V) yield a color-magnitude diagram with photometric errors of 1 sigma = 0.05 mag for stars with V less than 17.5, and 1 sigma approximately 0.1 mag at V = 20, which is 1.5 mag fainter than the main-sequence turnoff. Simulations show that the star identifications are essentially complete for stars with V less than 19.5, even in the densest regions of the cluster. The projected radial distribution of stars can be represented by a power law distribution: N(r) approximately r(exp alpha), with slope alpha = -0.4 +/- 0.15, into radii less than 0.4 sec (0.015 pc). The radial profile is also consistent with a flat core of any radius smaller than approximately 1.5 sec (0.05 pc), but inconsistent with any core radius larger than 2.5 sec (0.09 pc). A total of 30 blue straggler candidates within 20 sec of the cluster center have been identified from the three-color data. Their radial distribution is strongly centrally concentrated when compared to the horizontal branch, red giant branch, or main-sequence stars in the cluster. The abundance of blue stragglers (relative to red giants and subgiants) within r approximately less than 20 sec of the center of M30 is f(sub BS) = 0.19 =/- 0.04, about twice as high as in other dense clusters.

Yanny, Brian↗
NASA NTRS1995

Brightest cluster galaxies as standard candles

We investigate the use of brightest cluster galaxies (BCGs) as standard candles for measuring galaxy peculiar velocities on large scales. We have obtained precise large-format CCD surface photometry and redshifts for an all-sky, volume-limited (z less than or = 0.05) sample of 199 BCG. We reinvestigate the Hoessel (1980) relationship between the metric luminosity, L(sub m), within the central 10 kpc/h of the BCGs and the logarithmic slope of the surface brightness profile, alpha. The L(sub m)-alpha relationship reduces the cosmic scatter in L(sub m) from 0.327 mag to 0.244 mag, yielding a typical distance accuracy of 17% per BCG. Residuals about the L(sub m)-alpha relationship are independent of BCG luminosity, BCG B - R(sub c) color, BCG location within the host cluster, and richness of the host cluster. The metric luminosity is independent of cluster richness even before correcting for its dependence on alpha, which provides further evidence for the unique nature of the BCG luminosity function. Indeed, the BCG luminosity function, both before and after application of the alpha-correction, is consistent with a single Gaussian distribution. Half the BCGs in the sample show some evidence of small color gradients as a function of radius within their central 50 kpc/h regions but with almost equal numbers becoming redder as becoming bluer. However, with the central 10 kpc/h the colors are remarkably constant -- the mean B - R(sub c) color is 1.51 with a dispersion of only 0.06 mag. The narrow photometric and color distributions of the BCGs, the lack of 'second-parameter' effects, as well as the unique rich cluster environment of BCGs, argue that BCGs are the most homogeneous distance indicators presently available for large-scale structure research.

Postman, Marc↗
NASA NTRS1994

The Hubble Space Telescope extragalactic distance scale key project. 2: Photometry of WFC images of M81

The Extragalactic Distance Scale (H(sub o)) Key Project for Hubble Space Telescope (HST) aims to employ the Cepheid period-luminosity (P-L) relation to measure galaxy distances out as far as the Virgo Cluster. The vital steps in this program are (1) to obtain precise photometry of stellar images from the Wide Field Camera (WFC) exposures of selected galaxies, and (2) to calibrate this photometry to obtain reliable distances to these galaxies from the Cepheid P-L relation. We have used the DAOPHOT II and ALLFRAME programs to determine 28 instrumental magnitudes -- 22 of F555W (of about V) and six of F785LP (of about I) -- of all stars brighter than V of about 25 in each of two 2.56 arcmin x 2.56 arcmin WFC fields of M81. The reductions use a varying point-spread function to account for the field effects in the WFC optics and yield instrumental magnitudes with single epoch precision ranging from 0.09 to 0.24 mag, at V of about 21.8 to 23.8 -- the magnitude range of the 30 Cepheids that we have now identified in M81. For brighter stars (V of about 22), single epoch magnitudes are precise to 0.09 mag. The photometric calibration onto the Johnson V and Kron-Cousins I systems was determined from independent ground-based CCD observing at the Canada-France-Hawaii Telescope (CFHT) 3.6 m (confirmed by the Kitt Peak National Observatory (KPNO) 4.0 m) and from the Palomar 5.0 m (using the wide-field COSMIC camera) and 1.5 m telescopes. Secondary standards, taken from the COSMIC and CFHT frames, were established in each of the WFC fields in V and I, allowing a direct transformation from ALLFRAME magnitudes to calibrated V and I magnitudes, giving mean V of about 23 magnitudes accurate to of about +/- 0.1 mag. The stellar populations in M81 have been analyzed in terms of the luminosity functions and color magnitude diagrams (CMD) derived from these data, from which we identify numerous supergiants, and a CMD morphology similar to M33.

Hughes, Shaun M. G.↗
NASA NTRS1995

Analysis of Gaspra lightcurves using Galileo shape and photometric models

Galileo-based models for the shape of 951 Gaspra and the global-average photometric behavior of its surface have been used to model a representative subset of the asteroid's telescopic lightcurves. Fitting the synthetic lightcurves to the observed timing of lightcurve extrema, and knowing the orientation of Gaspra's axes at the time of the Galileo flyby, leads to a sidereal rotation period for the asteroid of 7.042024 +/- 0.000020 hr, a slight change from the period reported by Magnusson et al. (1992). Initially, the shapes, amplitudes, and absolute photometry of the synthetic and observed lightcurves agree with each other to within 0.05-0.1 mag. Small modifications to the Gaspra shape model on sides of the asteroid poorly imaged by Galileo (changes of 700 m or less in the southern hemisphere at longitudes 90 deg-270 deg W) reduce the typical discrepancies to approximately 0.05 mag in lightcurve shape and less than 0.03 mag in absolute photometry. The result demonstrates that Earth-based lightcurves can be used to refine the shape of a spacecraft-imaged irregular object in areas that are poorly constrained by the spacecraft observations. The consistency and phase-angle dependence of the Galileo-based model for Gaspra photometry, supports the accuracy of the absolute calibration of the Galileo SSI camera, and confirms the Earth-based determination of the V-filter geometric albedo of the asteroid (0.22 +/- 0.03; Tholen et al., submitted for publication). Remaining discrepancies between the synthetic and observed lightcurves show no indication of systematic latitudinal variations in albedo and also cannot be explained entirely by isolated albedo spots. These discrepancies are most likely caused by (1) small, remaining, hard-to-constrain errors in the Gaspra shape model and/or (2) moderate variations in macroscopic roughness across the asteroid's surface, in particular making longitudes 130 deg to 300 deg W moderately rougher than the opposite hemisphere.

Simonelli, Damon P.↗
NASA NTRS1997

Near Infrared Imaging of the Hubble Deep Field with Keck Telescope

Two deep K-band (2.2 micrometer) images, with point-source detection limits of K=25.2 mag (one sigma), taken with the Keck Telescope in subfields of the Hubble Deep Field, are presented and analyzed. A sample of objects to K=24 mag is constructed and V(sub 606)- I(sub 814) and I(sub 814)-K colors are measured. By stacking visually selected objects, mean I(sub 814)-K colors can be measured to very faint levels, the mean I(sub 814)-K color is constant with apparent magnitude down to V(sub 606)=28 mag.

Hogg, David W.↗
NASA NTRS1997

Kinematics and M(sub v) calibration of K and M dwarf stars using Hipparcos data

The luminosities and kinematics of lower main sequence stars in a spectroscopically selected sample covering spectral types K 3 to M 5 are determined using Hipparcos parallaxes and proper motions. The stars separate into two kinematically distinct components, called young disk and old disk components. The young component has velocity dispersion (30, 17, 12) km/s in the U, V and W directions, respectively, and features an asymmetric drift of 8 km/s, a vertex deviation of 10 +/- 3 deg and an absolute magnitude of 10.48 mag at color (R - I)(sub Kron) = 1.0 mag. The respective features of the old component are: (56, 34, 31) km/s, 28 km/s and 0.6 mag at the same color. The slope and intrinsic width of the magnitude calibration of each component are determined. The analysis is used to investigate the possible presence of residual systematic discrepancies of the model with Hipparcos data. There are indications of a possible underestimation of the parallax errors.

Upgren, A. R.↗
NASA NTRS2012

V344 Lyrae: A Touchtone SU UMa Cataclysmic Variable in the Kepler Field

We report on the analysis of the Kepler short-cadence (SC) light curve of V344 Lyr obtained during 2009 June 20 through 2010 Mar 19 (Q2-Q4). The system is an SU UMa star showing dwarf nova outbursts and superoutbursts, and promises to be a touchstone for CV studies for the foreseeable future. The system displays both positive and negative super humps with periods of 2.20 and 2.06-hr, respectively, and we identify an orbital period of 2.11-hr. The positive super humps have a maximum amplitude of approximately 0.025-mag, the negative super humps a maximum amplitude of approximately 0.8 mag, and the orbital period at quiescence has an amplitude of approximately 0.025 mag. The quality of the Kepler data is such that we can test vigorously the models for accretion disk dynamics that have been emerging in the past several years. The SC data for V344 Lyr are consistent with the model that two physical sources yield positive superhumps: early in the superoutburst. the superhump signal is generated by viscous dissipation within the periodically flexing disk. but late in the superoutburst, the signal is generated as the accretion stream bright spot sweeps around the rim of the non-axisymmetric disk. The disk super humps are roughly anti-phased with the stream/late superhumps. The V344 Lyr data also reveal negative super humps arising from accretion onto a tilted disk precessing in the retrograde direction, and suggest that negative superhumps may appear during the decline of DN outbursts. The period of negative superhumps has a positive P(raised dot) in between outbursts.

Smale, Alan P.↗
NASA NTRS2016

Global Energetics of Solar Flares: Nonthermal Energies - Part III

This study entails the third part of a global flare energetics project, in which Ramaty High-Energy Solar Spectroscopic Imager (RHESSI) data of 191 M and X-class flare events from the first 3.5 years of the Solar Dynamics Observatory mission are analyzed. We fit a thermal and a nonthermal component to RHESSI spectra, yielding the temperature of the differential emission measure (DEM) tail, the nonthermal power-law slope and flux, and the thermal nonthermal cross-over energy eco. From these parameters, we calculate the total nonthermal energy E(sub nt) in electrons with two different methods: (1) using the observed cross-over energy e(sub co) as low-energy cutoff, and (2) using the low-energy cut off e(sub wt) predicted by the warm thick-target bremsstrahlung model of Kontar et al. Based on a mean temperature of T(sub e) = 8.6 MK in active regions, we find low-energy cutoff energies of e(sub wt) = 6.2 +/-1.6 keV for the warm-target model, which is significantly lower than the cross-over energies e(sub co) = 21 +/- 6 keV. Comparing with the statistics of magnetically dissipated energies E(sub mag) and thermal energies E(sub th) from the two previous studies, we find the following mean (logarithmic) energy ratios with the warm-target model: E(sub nt) = 0.41E(sub mag), E(sub th) = 0.08 E(sub mag), and E(sub th) = 0.15 E(sub nt). The total dissipated magnetic energy exceeds the thermal energy in 95% and the nonthermal energy in 71% of the flare events, which confirms that magnetic reconnection processes are sufficient to explain flare energies. The nonthermal energy exceeds the thermal energy in 85% of the events, which largely confirms the warm thick-target model.

Aschwanden, Markus J.↗
NASA NTRS2020

Optical Follow-Up of the Neutron Star-Black Hole Mergers S200105ae and S200115j

LIGO and Virgo’s third observing run revealed the first neutron star–black hole (NSBH) merger candidates in gravitational waves. These events are predicted to synthesize r-process elements creating optical/near-infrared ‘kilonova’ emission. The joint gravitational wave and electromagnetic detection of an NSBH merger could be used to constrain the equation of state of dense nuclear matter, and independently measure the local expansion rate of the Universe. Here, we present the optical follow-up and analysis of two of the only three high-significance NSBH merger candidates detected to date, S200105ae and S200115j, with the Zwicky Transient Facility. The Zwicky Transient Facility observed ~48% of S200105ae and ~22% of S200115j’s localization probabilities, with observations sensitive to kilonovae brighter than −17.5 mag fading at 0.5 mag d−1 in the g- and r-bands; extensive searches and systematic follow-up of candidates did not yield a viable counterpart. We present state-of-the-art kilonova models tailored to NSBH systems that place constraints on the ejecta properties of these NSBH mergers. We show that with observed depths of apparent magnitude ~22 mag, attainable in metre-class, wide-field-of-view survey instruments, strong constraints on ejecta mass are possible, with the potential to rule out low mass ratios, high black hole spins and large neutron star radii.

Shreya Anand↗
NASA NTRS2021

The Enigmatic Brown Dwarf WISEA J153429.75-104303.3 (a.k.a. "The Accident")

Continued follow-up of WISEA J153429.75−104303.3, announced in Meisner et al., has proven it to have an unusual set of properties. New imaging data from Keck/MOSFIRE and HST/WFC3 shows that this object is one of the few faint proper motion sources known withJ−ch2>8 mag, indicating a very cold temperature consistent with the latest known Y dwarfs. Despite this, it has W1−W2 and ch1−ch2 colors∼1.6 mag bluer than a typical Y dwarf. A new trigonometric parallax measurement from a combination of WISE, Spitzer, and HST astrometry confirms a nearby distance of-+16.31.21.4pc and a large transverse velocity of 207.4±15.9 km s−1. The absoluteJ,W2, and ch2 magnitudes are in line with the coldest known Y dwarfs, despite the highly discrepant W1−W2 andch1−ch2 colors. We explore possible reasons for the unique traits of this object and conclude that it is most likely an old, metal-poor brown dwarf and possibly the first Y subdwarf. Given that the object has an HST F110Wmagnitude of 24.7 mag, broadband spectroscopy and photometry from JWST are the best options for testing this hypothesis

J Davy Kirkpatrick↗
DOE OSTI2021

Fungal-Bacterial Cooccurrence Patterns Differ between Arbuscular Mycorrhizal Fungi and Nonmycorrhizal Fungi across Soil Niches

Soil bacteria and fungi are known to form niche-specific communities that differ between actively growing and decaying roots. Yet almost nothing is known about the cross-kingdom interactions that frame these communities and the environmental filtering that defines these potentially friendly or competing neighbors. We explored the temporal and spatial patterns of soil fungal (mycorrhizal and nonmycorrhizal) and bacterial cooccurrence near roots of wild oat grass, Avena fatua, growing in its naturalized soil in a greenhouse experiment. Amplicon sequences of the fungal internal transcribed spacer (ITS) and bacterial 16S rRNA genes from rhizosphere and bulk soils collected at multiple plant growth stages were used to construct covariation-based networks as a step toward identifying fungal-bacterial associations. Corresponding stable-isotope-enabled metagenome-assembled genomes (MAGs) of bacteria identified in cooccurrence networks were used to inform potential mechanisms underlying the observed links. Bacterial-fungal networks were significantly different in rhizosphere versus bulk soils and between arbuscular mycorrhizal fungi (AMF) and nonmycorrhizal fungi. Over 12 weeks of plant growth, nonmycorrhizal fungi formed increasingly complex networks with bacteria in rhizosphere soils, while AMF more frequently formed networks with bacteria in bulk soils. Analysis of network-associated bacterial MAGs suggests that some of the fungal-bacterial links that we identified are potential indicators of bacterial breakdown and consumption of fungal biomass, while others intimate shared ecological niches.

54 ENVIRONMENTAL SCIENCES↗
DOE OSTI2025

Metagenome-assembled genomes measured at 3 depths during snowmelt period in East River, CO (March, May, and June, September 2017)

Snowmelt is a critical biogeochemical period that accounts for large nitrogen (N) export events from high-elevation watersheds. Soil microbial populations bloom and immobilize N during snowmelt, yet the population size crashes in spring, which releases a pulse of soil N. We sought to discover the N sources fueling this microbial bloom and determine the fate of N following microbial die-off. Here, focusing on the snowmelt period within a headwater catchment of the Upper Colorado River Basin (East River, CO), we deployed strain-resolved metagenomics to identify the metabolic pathways and processes that mobilize soil N during and after snowmelt. Soil metagenome samples were taken from 6 snowpits from 3 depths (0-5cm, 5-15cm, >15cm) at 4 time points during snowmelt period (March 2017, May 2017, and June 2017, September 2017) generating 48 metagenomes. We reconstructed 474 metagenome-assembled genomes (MAGs) across all metagenomes.All 48 metagenomes were sequenced at JGI and raw data can be found under JGI (Joint Genome Institute) GOLD Study Gs0135149. Metagenome assemblies from IMG under the same study were used for genome binning. This dataset (1) a zip file of 474 MAGs (as fasta files, Gs0135149_bins_tar.gz), (2) sample metadata file with sample IGSNs (International Generic Sample Numbers) (samples.csv), (3) bounding box coordinates for the sampled locations (Gs0135149.kml), (4) metagenome metadata file listing IMG/M (Integrated Microbial Genomes/Metagenomes) metagenome accessions linking samples to metagenomes (metagenomes.csv), (5) location metadata file (locations.csv), (6) file-level metadata file (flmd.csv) and (7) data dictionary (dd.csv) file.This work was supported by the Watershed Function Science Focus Area at Lawrence Berkeley National Laboratory funded by the US Department of Energy, Office of Science, Biological and Environmental Research under Contract No. DE-AC02-05CH11231.

54 ENVIRONMENTAL SCIENCES↗
DOE OSTI2025

Metagenome-assembled genomes from topsoils along a hillslope water gradient across early snowmelt to late summer in East River, CO

Drought is changing the American Mountain West at unprecedented rates with unknown consequences to soil microbiome composition and function. As a part of LBNL Watershed Science Focus Area (SFA), we investigated shifts in microbial community and transcriptional activity on a subalpine conifer-meadow transition zone throughout the summer of 2023 as soil dried down. This work took place in Crested Butte, CO on Snodgrass mountain, using a proxy for drought conditions.Here we present metagenome assembled genomes (MAGs) for the bacterial and archaeal community at 0-10cm from three sites along a hillslope water gradient across five timepoints from early snowmelt to late summer. 42 metagenomes were sequenced at Joint Genome Institute (JGI) and can be found under the JGI GOLD (Genomes Online Database) sequencing project Gs0166660. Metagenomes were assembled through an inhouse pipeline (see methods), binned using four autobinners (concoct, maxbin2, metabat2, and vamb) and consolidated using dastool. The consolidated bins from all metagenomes were pooled, filtered by completeness (>70%) and contamination (<10%), and dereplicated at 95% ANI using drep. This dataset (1) a zip file of 157 MAGs (as fasta files, Gs0166660_bins_tar.gz), (2) sample metadata file with sample IGSNs (International Generic Sample Numbers) (samples.csv), (3) bounding box coordinates for the sampled locations (Gs0166660.kml), (4) metagenome assembly and coassembly metadata file listing IMG/M (Integrated Microbial Genomes/Metagenomes) metagenome accessions linking samples to metagenomes (EastRiver_Drought_ESSDive_Metadata.csv), (5) location metadata file (locations.csv), (6) file-level metadata file (flmd.csv) and (7) data dictionary (dd.csv) file.This work was supported by the Watershed Function Science Focus Area at Lawrence Berkeley National Laboratory funded by the US Department of Energy, Office of Science, Biological and Environmental Research under Contract No. DE-AC02-05CH11231.

54 ENVIRONMENTAL SCIENCES↗
DOE OSTI2025

Metagenome-assembled genomes from topsoils collected during NEON campaign in East River, CO (06/14/2018-06/28/2018)

The Watershed Function Science Focus Area (WF SFA) at Lawrence Berkeley National Lab is working to build a mechanistic understanding of the distribution and dynamics of biogeochemical processes in mountainous watersheds and their response to perturbation. In June 2018, the NEON (National Ecological Observatory Network) Airborne Observatory Platform (AOP) performed a taskable airborne imaging campaign to collect visible to shortwave infrared (VSWIR) imaging spectroscopy and LiDAR data across 330 km2 in the Upper East River at Crested Butte, CO. We conducted a parallel ground sampling campaign to sample vegetation traits, as well as soil physical, chemical, and microbiological characteristics. We collected these samples from 438 sites across 12 locations spanning much of the elevation, topographic, and geologic variability across the study area. A subset of 250 samples were used for soil metagenomics which is presented here. In addition, at each site, vegetation samples were collected to measure species-specific leaf water content and leaf mass area, foliar elemental composition and foliar CN stable isotope ratios. Soil samples were collected to measure soil physical properties which include bulk density and soil texture analysis. A suite of soil chemical properties was measured from the samples collected at each site, including pH, organic matter, concentrations exchangeable cations, total elemental composition, and the concentrations of extractable N pools (e.g. total free amino acids, ammonium, nitrate, dissolved organic N, and total dissolved N). Additionally, we have measured soil microbial biomass CN stoichiometry. Here, we present 1982 metagenome-assembled genomes (MAGs) for the bacterial and archaeal community from topsoil collected from during NEON 2018 campaign. All metagenomes were sequenced at JGI (Joint Genome Institute) (GOLD Study ID: Gs0149986). Metagenomes were assembled using JGI Metagenome Workflow (10.1128/mSystems.00804-20). The dataset includes (1) zip files for 1982 MAG fasta files (neon_genomes1-5.tar.gz, split into 5 tarballs to keep tarballs under 0.5 GB), (2) neon_Gs0149986_samples_soilproperties_metagenomes.csv: the sample information together with the accession numbers for the underlying metagenomes and the associated soil physical and chemical measurements in NMDC (National Microbiome Data Collaborative) compliant format, (3) neon_Gs0149986.kml: location bounding box file for the sampled locations, (4) samples.csv: sample metadata file used to register Internationall Generic Sample Numbers (IGSNs), (5) flmd.csv: file level metadata file, and (6) dd.csv: data dictionary file. This work was supported by the Watershed Function Science Focus Area at Lawrence Berkeley National Laboratory funded by the US Department of Energy, Office of Science, Biological and Environmental Research under Contract No. DE-AC02-05CH11231.

2018 NEON and 2025 CHESS Campaigns↗
DOE OSTI2023

Long-term CRISPR locus dynamics and stable host-virus co-existence in subsurface fractured shales

Viruses are the most ubiquitous biological entities on Earth. Even so, elucidating the impact of viruses on microbial communities and associated ecosystem processes often requires identification of unambiguous host-virus linkages-an undeniable challenge in many ecosystems. Subsurface fractured shales present a unique opportunity to first make these strong linkages via spacers in CRISPR-Cas arrays and subsequently reveal complex long-term host-virus dynamics. Here, we sampled two replicated sets of fractured shale wells for nearly 800 days, resulting in 78 metagenomes from temporal sampling of six wells in the Denver-Julesburg Basin (Colorado, USA). At the community level, there was strong evidence for CRISPR-Cas defense systems being used through time and likely in response to viral interactions. Within our host genomes, represented by 202 unique MAGs, we also saw that CRISPR-Cas systems were widely encoded. Together, spacers from host CRISPR loci facilitated 2,110 CRISPR-based viral linkages across 90 host MAGs spanning 25 phyla. We observed less redundancy in host-viral linkages and fewer spacers associated with hosts from the older, more established wells, possibly reflecting enrichment of more beneficial spacers through time. Leveraging temporal patterns of host-virus linkages across differing well ages, we report how host-virus co-existence dynamics develop and converge through time, possibly reflecting selection for viruses that can evade host CRISPR-Cas systems. Together, our findings shed light on the complexities of host-virus interactions as well as long-term dynamics of CRISPR-Cas defense among diverse microbial populations.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2022

Expanding the genomic encyclopedia of Actinobacteria with 824 isolate reference genomes

The phylum Actinobacteria includes important human pathogens like Mycobacterium tuberculosis and Corynebacterium diphtheriae and renowned producers of secondary metabolites of commercial interest, yet only a small part of its diversity is represented by sequenced genomes. Here, we present 824 actinobacterial isolate genomes in the context of a phylum-wide analysis of 6,700 genomes including public isolates and metagenome-assembled genomes (MAGs). We estimate that only 30%–50% of projected actinobacterial phylogenetic diversity possesses genomic representation via isolates and MAGs. A comparison of gene functions reveals novel determinants of host-microbe interaction as well as environment-specific adaptations such as potential antimicrobial peptides. We identify plasmids and prophages across isolates and uncover extensive prophage diversity structured mainly by host taxonomy. Analysis of >80,000 biosynthetic gene clusters reveals that horizontal gene transfer and gene loss shape secondary metabolite repertoire across taxa. Our observations illustrate the essential role of and need for high-quality isolate genome sequences.

59 BASIC BIOLOGICAL SCIENCES↗