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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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At least 235 records · Page 13
All sourcesNASA NTRSDOE OSTI
NASA NTRS2016

Biospecimen Retrieval from NASA's Rodent Research-1: Maximizing Science Return from Flight Missions

Rodent Research (RR)-1 was conducted to validate flight hardware, operations, and science capabilities that were developed to support long duration missions on the International Space Station. After 37 days in microgravity twenty mice were euthanized and frozen on orbit. Upon return to Earth the carcasses were dissected and yielded 32 different types of tissues from each mouse and over 3200 tissue aliquots. Many tissues were distributed to the Space Life and Physical Sciences (SLPS) Biospecimen Sharing Program (BSP) Principal Investigators (PIs) through the Ames Life Science Data Archive (ALSDA). A second round of dissections was performed to collect additional tissues from the remaining carcasses thawed for a second time for additional BSP PIs. Tissues retrieved included vaginal walls, aorta, pelvis, brown adipose tissue, tail, spine and forearms. Although the analyses are still in progress, some of the PIs have reported that the quality of the tissues was acceptable for their study. In a separate experiment we tested the RNA quality of the tissues that were dissected from frozen carcasses that were subjected to euthanasia, freezing, first and second thaw dissections. Timelines simulated the on-orbit RR-1 procedures to assess the quality of the tissues retrieved from the second thaw dissections. We analyzed the RIN values of select tissues including kidney, brain, white adipose tissue (WAT) and brown adipose tissue (BAT). Overall the RIN values from the second thaw were lower compared to those from the first by about a half unit; however, the tissues yielded RNA that are acceptable quality for some quantitative gene expression assays. Interestingly, RIN values of brain tissues were 8.4+/-0.6 and 7.9+/-0.7 from first and second round dissections, respectively (n5). Kidney and WAT yielded RIN values less than 8 but they can still be used for qPCR. BAT yielded higher quality RNA (8.2+/-0.5) than WAT (5.2+/-20.9), possibly due to the high fat content. Together, these data show that select tissues can be utilized for gene expression studies even if they are retrieved from carcasses that were subjected to at least two freezing and thawing processes; this further expands science return from valuable and infrequent rodent experiments in space.

rodents↗
NASA NTRS2016

Biospecimen Retrieval from NASA's Rodent Research-1: Maximizing Science Return from Flight Missions

Rodent Research (RR)-1 was conducted to validate flight hardware, operations, and science capabilities that were developed to support long duration missions on the International Space Station. After 37 days in microgravity twenty mice were euthanized and frozen on orbit. Upon return to Earth the carcasses were dissected and yielded 32 different types of tissues from each mouse and over 3200 tissue aliquots. Many tissues were distributed to the Space Life and Physical Sciences (SLPS) Biospecimen Sharing Program (BSP) Principal Investigators (PIs) through the Ames Life Science Data Archive (ALSDA). A second round of dissections was performed to collect additional tissues from the remaining carcasses thawed for a second time for additional BSP PIs. Tissues retrieved included vaginal walls, aorta, pelvis, brown adipose tissue, tail, spine and forearms. Although the analyses are still in progress, some of the PIs have reported that the quality of the tissues was acceptable for their study. In a separate experiment we tested the RNA quality of the tissues that were dissected from frozen carcasses that were subjected to euthanasia, freezing, first and second thaw dissections. Timelines simulated the on-orbit RR-1 procedures to assess the quality of the tissues retrieved from the second thaw dissections. We analyzed the RIN values of select tissues including kidney, brain, white adipose tissue (WAT) and brown adipose tissue (BAT). Overall the RIN values from the second thaw were lower compared to those from the first by about a half unit; however, the tissues yielded RNA that are acceptable quality for some quantitative gene expression assays. Interestingly, RIN values of brain tissues were 8.4+/-0.6 and 7.9+/-0.7 from first and second round dissections, respectively (n=5). Kidney and WAT yielded RIN values less than 8 but they can still be used for qPCR. BAT yielded higher quality RNA (8.2+/-0.5) than WAT (5.22+/-0.9), possibly due to the high fat content. Together, these data show that select tissues can be utilized for gene expression studies even if they are retrieved from carcasses that were subjected to at least two freezing and thawing processes; this further expands science return from valuable and infrequent rodent experiments in space.

rodent↗
NASA NTRS2018

Columbus IFHX Ammonia Leak Analysis

After the Columbus Moderate Temperature Loop (MTL) InterFace Heat eXchanger (IFHX) low temperature event of GMT 345-2013, NASA investigated relevant transient scenarios involving IFHX rupture after water freezing and subsequent thawing. NASA recommended development of a Fault Detection Isolation and Recovery (FDIR) plan that would, in the event of a heat exchanger freeze event, close the Water On/Off Valves (WOOVs) to isolate the heat exchanger and prevent ammonia from the external flow loops from spreading into the cabin. NASA performed a preliminary simplified analysis for the reference case of IFHX rupture, but for a deeper understanding TAS developed detailed SINDA-FLUINT models of the Columbus ITCS that were built and run through the SINAPS GUI. This allowed simulation of the ammonia leakage physics including the variation of environmental parameters, thus providing more accurate and specific input to the FDIR under development. The result was finalization of the IFHX WOOVs closure sequence and wait times to contain the ammonia propagation to Columbus and allow identification of the leaking IFHX. In addition, the analysis results provided reference pressure profiles to be used on console and by the Engineering as support for the telemetry data assessment in case of failure.This paper gives an overview on the issue and focuses on the analytical aspects of the multiphase fluid dynamics involved.

Tilloca, A.↗
DOE OSTI2026

Hydrological and Thermal Dynamics of a Supra‐Permafrost Subterranean Estuary

Subterranean estuaries (STEs), where groundwater interacts with seawater, influence surface and subsurface coastal ecology and biogeochemistry. In Arctic-STEs overlying permafrost, groundwater flow and heat transport determine the fate of organic matter. Yet, direct observations of groundwater flow and heat and solute transport processes in Arctic STEs remain limited. This study characterized groundwater flow paths and fluxes and heat transport within an Arctic-STE along Alaska's Beaufort Sea coast during thawing, summer, and freeze-up. Intertidal seabed temperature-depth profiles collected along a 10-m transect captured the active groundwater flow period, from thaw and flow onset in mid-June to freeze-up in late-September. During this period, aquifer geometry evolved non-uniformly due to spatially varying thaw rates across the STE (mean (m) thaw depths–beach: 0.25 to 0.55–0.6 m on 20 June, 25 July, 1 October; seabed: 0.6–0.9 m from 25 July to 1 October). Groundwater and surface water levels, salinity, and subsurface temperature profiles measured over tidal time scales were interpreted alongside groundwater flow-heat transport numerical simulations. Fresh groundwater discharge was sporadic during thawing (m: 0.32 m 3 /day/m), abundant in summer (m: 0.45 m 3 /day/m), and was largely absent during freeze-up. During freeze-up, groundwater flow was driven exclusively by seawater recirculation via tidal pumping (from thawing to summer to freeze-up: 0.00025–0.15–0.5 m3/day/m) and convection. Heat advection dominated near aquatic interfaces (shaping intertidal ice), and conduction controlled vertical temperature gradients in low-flow and unsaturated sediments. These findings will help predict how prolonged summers will alter Arctic-STE cryo-hydrology and biogeochemistry.

54 ENVIRONMENTAL SCIENCES↗
NASA NTRS1976

Storing Blood Cells

The National Cancer Institute worked with Goddard Space Flight Center to propose a solution to the blood-cell freezing problem. White blood cells and bone marrow are stored for future use by leukemia patients as a result of Goddard and Jet Propulsion Laboratory expertise in electronics and cryogenics. White blood cell and bone marrow bank established using freezing unit. Freezing unit monitors temperature of cells themselves. Thermocouple placed against polyethylene container relays temperature signals to an electronic system which controls small heaters located outside container. Heaters allow liquid nitrogen to circulate at constant temperature and maintain consistent freezing rate. Ability to freeze, store, and thaw white cells and bone marrow without damage is important in leukemia treatment.

Source record↗
NASA NTRS2008

Investigation of Condensing Ice Heat Exchangers for MTSA Technology Development

Metabolic heat regenerated Temperature Swing Adsorption (MTSA) technology is being developed for thermal, carbon dioxide (CO2) and humidity control for a Portable Life Support Subsystem (PLSS). Metabolically-produced CO2 present in the ventilation gas of a PLSS is collected using a CO2selective adsorbent via temperature swing adsorption. The temperature swing is initiated through cooling to well below metabolic temperatures. Cooling is achieved with a sublimation heat exchanger using water or liquid carbon dioxide (LCO2) expanded below sublimation temperature when exposed to low pressure or vacuum. Subsequent super heated vapor, as well as additional coolant, is used to further cool the astronaut. The temperature swing on the adsorbent is then completed by warming the adsorbent with a separate condensing ice heat exchanger (CIHX) using metabolic heat from moist ventilation gas. The condensed humidity in the ventilation gas is recycled at the habitat. The water condensation from the ventilation gas is a significant heat transfer mechanism for the warming of the adsorbent bed because it represents as much as half of the energy potential in the moist ventilation gas. Designing a heat exchanger to efficiently transfer this energy to the adsorbent bed and allow the collection of the water is a challenge since the CIHX will operate in a temperature range from 210K to 280K. The ventilation gas moisture will first freeze and then thaw, sometimes existing in three phases simultaneously. A NASA Small Business Innovative Research (SBIR) Phase 1 contract was performed to investigate condensing and icing as applied to MTSA to enable higher fidelity modeling and assess the impact of geometry variables on CIHX performance for future CIHX design optimization. Specifically, a design tool was created using analytical relations to explore the complex, interdependent design space of a condensing ice heat exchanger. Numerous variables were identified as having nontrivial contributions to performance such as hydraulic diameter, heat exchanger effectiveness, ventilation gas mass flow rate and surface roughness. Using this tool, four test articles were designed and manufactured to map to a full MTSA subassembly (the adsorbent bed, the sublimation heat exchanger for cooling and the condensing ice heat exchanger for warming). The design mapping considered impacts due to CIHX geometry as well as subassembly impacts such as thermal mass and thermal resistance through the adsorbent bed. The test articles were tested at simulated PLSS ventilation loop temperature, moisture content and subambient pressure. Ice accumulation and melting were observed. Data and test observations were analyzed to identify drivers of the condensing ice heat exchanger performance. This paper will discuss the analytical models, the test article designs, and testing procedures. Testing issues will be discussed to better describe data and share lessons learned. Data analysis and subsequent conclusions will be presented.

Padilla, Sebastian↗
NASA NTRS2009

Alternative OTEC Scheme for a Submarine Robot

A proposed system for exploiting the ocean thermal gradient to generate power would be based on the thawing-expansion/ freezing-contraction behavior of a wax or perhaps another suitable phase-change material. The power generated by this system would be used to recharge the batteries in a battery-powered unmanned underwater vehicle [UUV (essentially, a small exploratory submarine robot)] of a type that has been deployed in large numbers in research pertaining to global warming. A UUV of this type travels between the ocean surface and various depths, measuring temperature and salinity. This proposed system would be an alternative to another proposed ocean thermal energy conversion (OTEC) system that would serve the same purpose but would utilize a thermodynamic cycle in which CO2 would be the working fluid. That system is described in Utilizing Ocean Thermal Energy in a Submarine Robot (NPO-43304), immediately following this brief. The main advantage of this proposed system over the one using CO2 is that it could derive a useful amount of energy from a significantly smaller temperature difference. At one phase of its operational cycle, the system now proposed would utilize the surface ocean temperature (which lies between 15 and 20 C over most of the Earth) to melt a wax (e.g., pentadecane) that has a melting/freezing temperature of about 10 C. At the opposite phase of its operational cycle, the system would utilize the lower ocean temperature at depth (e.g., between 4 and 7 C at a depth of 300 m) to freeze the wax. The melting or freezing causes the wax to expand or contract, respectively, by about 8 volume percent.

Jones, Jack↗
NASA NTRS2009

Investigation of Condensing Ice Heat Exchangers for MTSA Technology Development

Metabolic heat regenerated Temperature Swing Adsorption (MTSA) technology is being developed for thermal, carbon dioxide (CO2) and humidity control for a Portable Life Support Subsystem (PLSS). Metabolically-produced CO2 present in the ventilation gas of a PLSS is collected using a CO2-selective adsorbent via temperature swing adsorption. The temperature swing is initiated through cooling to well below metabolic temperatures. Cooling is achieved with a sublimation heat exchanger using water or liquid carbon dioxide (L CO2) expanded below sublimation temperature when exposed to low pressure or vacuum. Subsequent super heated vapor, as well as additional coolant, is used to further cool the astronaut. The temperature swing on the adsorbent is then completed by warming the adsorbent with a separate condensing ice heat exchanger (CIHX) using metabolic heat from moist ventilation gas. The condensed humidity in the ventilation gas is recycled at the habitat. The water condensation from the ventilation gas represents a significant source of potential energy for the warming of the adsorbent bed as it represents as much as half of the energy potential in the moist ventilation gas. Designing a heat exchanger to efficiently transfer this energy to the adsorbent bed and allow the collection of the water is a challenge since the CIHX will operate in a temperature range from 210K to 280K. The ventilation gas moisture will first freeze and then thaw, sometimes existing in three phases simultaneously.

Padilla, Sebastian↗
NASA NTRS2013

Positive-Buoyancy Rover for Under Ice Mobility

A buoyant rover has been developed to traverse the underside of ice-covered lakes and seas. The rover operates at the ice/water interface and permits direct observation and measurement of processes affecting freeze- over and thaw events in lake and marine environments. Operating along the 2- D ice-water interface simplifies many aspects of underwater exploration, especially when compared to submersibles, which have difficulty in station-keeping and precision mobility. The buoyant rover consists of an all aluminum body with two aluminum sawtooth wheels. The two independent body segments are sandwiched between four actuators that permit isolation of wheel movement from movement of the central tether spool. For normal operations, the wheels move while the tether spool feeds out line and the cameras on each segment maintain a user-controlled fixed position. Typically one camera targets the ice/water interface and one camera looks down to the lake floor to identify seep sources. Each wheel can be operated independently for precision turning and adjustments. The rover is controlled by a touch- tablet interface and wireless goggles enable real-time viewing of video streamed from the rover cameras. The buoyant rover was successfully deployed and tested during an October 2012 field campaign to investigate methane trapped in ice in lakes along the North Slope of Alaska.

Leichty, John M.↗
DOE OSTI2020

NGEE Arctic CO2, CH4 and Energy Eddy-Covariance (EC) Flux Tower Auxiliary Measurements, Utqiagvik (Barrow), Alaska, 2012 - 2019

Measurements began in September 2012 and are generally made throughout the snow-free growing season starting from April (pre-thawing) until November (freeze-up) when the instrumentation is taken down for maintenance and instrument calibration. CO2, CH4 and energy fluxes using the Eddy Covariance (EC) technique (Baldocchi 2003) and meteorological measurements are reported as 30-minute averages. The tower site is registered with AmeriFlux as US-NGB. See http://ameriflux.lbl.gov/sites/siteinfo/US-NGB for more information. Reported data include: (1) additional/extra meteorological and eddy covariance CO2, CH4 and energy flux data, (2) binned spectra for the three wind components, the sonic temperature and gas densities together with the binned cospectra for covariances of w (vertical wind component) and gas densities, (3) binned ogives (cumulative (co)spectra) for the three wind components, the sonic temperature and gas densities, and (4) tower site footprint matrices. Meteorological and eddy covariance CO2, CH4 and energy flux data (AmeriFlux BASE data product) are available from AmeriFlux https://doi.org/10.17190/AMF/1436326. The tower site is adjacent to the NGEE Arctic Tram system with several complementary time-series measurements https://doi.org/10.5440/1558782.This dataset supersedes the previously released dataset "Eddy-Covariance and auxiliary measurements, NGEE-Barrow, 2012-2013" DOI:10.5440/1124200 as this new product includes data for the longer time period processed using consistent methods for the entire record. The Next-Generation Ecosystem Experiments: Arctic (NGEE Arctic), was a research effort to reduce uncertainty in Earth System Models by developing a predictive understanding of carbon-rich Arctic ecosystems and feedbacks to climate. NGEE Arctic was supported by the Department of Energy's Office of Biological and Environmental Research. The NGEE Arctic project had two field research sites: 1) located within the Arctic polygonal tundra coastal region on the Barrow Environmental Observatory (BEO) near Utqiagvik (Barrow), Alaska and 2) multiple areas in the discontinuous permafrost region of the Seward Peninsula north of Nome, Alaska. Through observations, experiments, and synthesis with existing datasets, NGEE Arctic provided an enhanced knowledge base for multi-scale modeling and contributed to improved process representation at global pan-Arctic scales within the Department of Energy's Earth system Model (the Energy Exascale Earth System Model, or E3SM), and specifically within the E3SM Land Model component (ELM).

54 ENVIRONMENTAL SCIENCES↗
NASA NTRS1998

Seasonally Frozen Soil Monitoring Using Passive Microwave Satellite Data and Simulation Modeling

Satellite data and simulation modeling were used to assess seasonally frozen soils in the central US - Canada borders area (46-53 degrees N and 96-108 degrees). We used Scanning Multichannel Microwave Radiometer (SMMR) satellite data to delineate the top layer of frozen soils. SMMR is a passive microwave sensor having five channels (6.6, 10, 18, 21 and 37 GHz) with a horizontal and vertical polarization. SMRR data are available between 1978-1987 with noon and midnight overpass and footprint sizes between 25 km and 150 km. SMMR data were processed from resampled 1/4 degree grid cells during fall freeze-up and spring thaw (fall 1985 - spring 1987). The dielectric properties of a target may directly affect the satellite signal. The dielectric value is an order of magnitude smaller for frozen soil water. There are other significant changes to the emitted microwave signal from changes to the surface physical temperature, attenuation of the soil signal from plant water and soil moisture. We further characterized the temporal and spatial dynamic of frozen soils using the FroST (Frozen Soil Temperature) simulation model. The FroST model was used to further predict soil water and ice content, and soil temperature. SMMR results were compared versus 5-cm soil temperature data from available weather stations (14 in Canada and 11 for available months in the US). SMMR data were analyzed as a function of frequency, polarization, polarization difference, and "frequency gradient". In addition, vegetation density, physical temperature and snow depth were also considered. Preliminary analysis of SMMR derived frozen soil/thaw classification using a simple threshold classification indicates a mean overall classification accuracy by season of 85 percent. A sensitivity analysis for different soils with varying amounts of snow was conducted with FroST, which showed that the amount of snow, and the time of snow fall and melt affected the ice and water content, and depth of thaw. These results indicate a potential source of flooding and erosion under conditions when melting snow and spring rains provide a source of infiltrating water.

Toll, D. L.↗
NASA NTRS2015

Evaluation of Spaceborne L-band Radiometer Measurements for Terrestrial Freeze/Thaw Retrievals in Canada

The landscape freeze/thaw (FT) state has an important impact on the surface energy balance, carbon fluxes, and hydrologic processes; the timing of spring melt is linked to active layer dynamics in permafrost areas. L-band (1.4 GHz) microwave emission could allow the monitoring of surface state dynamics due to its sensitivity to the pronounced permittivity difference between frozen and thawed soil. The aim of this paper is to evaluate the performance of both Aquarius and Soil Moisture and Ocean Salinity (SMOS) L-band passive microwave measurements using a polarization ratio-based algorithm for landscape FT monitoring. Weekly L-band satellite observations are compared with a large set of reference data at 48 sites across Canada spanning three environments: tundra, boreal forest, and prairies. The reference data include in situ measurements of soil temperature (Tsoil) and air temperature (Tair), and Moderate Resolution Imaging Spectroradiometer (MODIS) land surface temperature (LST) and snow cover area (SCA) products. Results show generally good agreement between Lband FT detection and the surface state estimated from four reference datasets. The best apparent accuracies for all seasons are obtained using Tair as the reference. Aquarius radiometer 2 (incidence angle of 39.6) data gives the best accuracies (90.8), while for SMOS the best results (87.8 of accuracy) are obtained at higher incidence angles (55- 60). The FT algorithm identifies both freeze onset and end with a delay of about one week in tundra and two weeks in forest and prairies, when compared to Tair. The analysis shows a stronger FT signal at tundra sites due to the typically clean transitions between consistently frozen and thawed conditions (and vice versa) and the absence of surface vegetation. Results in the prairies were poorer because of the influence of vegetation growth in summer (which decreases the polarization ratio) and the high frequency of ephemeral thaw events during winter. Freeze onset and end maps created from the same algorithm applied to SMOS and Aquarius measurements characterize similar FT patterns over Canada. This study shows the potential of using L-band spaceborne observations for FT monitoring, but underlines some limitations due to ice crusts in the snowpack, liquid water content in snow cover during the spring freeze to thaw transition, and vegetation growth.

soil freeze/thaw↗
DOE OSTI2021

Projecting Permafrost Thaw of Sub-Arctic Tundra With a Thermodynamic Model Calibrated to Site Measurements

Northern circumpolar permafrost thaw affects global carbon cycling, as large amounts of stored soil carbon becomes accessible to microbial breakdown under a warming climate. The magnitude of carbon release is linked to the extent of permafrost thaw, which is locally variable and controlled by soil thermodynamics. Soil thermodynamic properties, such as thermal diffusivity, govern the reactivity of the soil-atmosphere thermal gradient, and are controlled by soil composition and drainage. In order to project permafrost thaw for an Alaskan tundra experimental site, we used seven years of site data to calibrate a soil thermodynamic model using a data assimilation technique. The model reproduced seasonal and interannual temperature dynamics for shallow (5–40 cm) and deep soil layers (2–4 m), and simulations of seasonal thaw depth closely matched observed data. The model was then used to project permafrost thaw at the site to the year 2100 using climate forcing data for three future climate scenarios (RCP 4.5, 6.0, and 8.5). Minimal permafrost thawing occurred until mean annual air temperatures rose above the freezing point, after which we measured over a 1 m increase in thaw depth for every 1 °C rise in mean annual air temperature. Under no projected warming scenario was permafrost remaining in the upper 3 m of soil by 2100. We demonstrated an effective data assimilation method that optimizes parameterization of a soil thermodynamic model. The sensitivity of local permafrost to climate warming illustrates the vulnerability of sub-Arctic tundra ecosystems to significant and rapid soil thawing.

54 ENVIRONMENTAL SCIENCES↗
NASA NTRS1995

Folic acid content in thermostabilized and freeze-dried space shuttle foods

This study was designed to determine whether freeze-dried and thermostabilized foods on a space shuttle contain adequate folate and to investigate any effects of freeze-drying on folacin. Frozen vegetables were analyzed after three states of processing: thawed; cooked; and rehydrated. Thermostabilized items were analyzed as supplied with no further processing. Measurable folate decreased in some freeze-dried vegetables and increased in others. Folacin content of thermostabilized food items was comparable with published values. We concluded that although the folacin content of some freeze-dried foods was low, adequate folate is available from the shuttle menu to meet RDA guidelines.

NASA Center JSC↗
NASA NTRS1995

HPTAM, a two-dimensional Heat Pipe Transient Analysis Model, including the startup from a frozen state

A two-dimensional Heat Pipe Transient Analysis Model, 'HPTAM,' was developed to simulate the transient operation of fully-thawed heat pipes and the startup of heat pipes from a frozen state. The model incorporates: (a) sublimation and resolidification of working fluid; (b) melting and freezing of the working fluid in the porous wick; (c) evaporation of thawed working fluid and condensation as a thin liquid film on a frozen substrate; (d) free-molecule, transition, and continuum vapor flow regimes, using the Dusty Gas Model; (e) liquid flow and heat transfer in the porous wick; and (f) thermal and hydrodynamic couplings of phases at their respective interfaces. HPTAM predicts the radius of curvature of the liquid meniscus at the liquid-vapor interface and the radial location of the working fluid level (liquid or solid) in the wick. It also includes the transverse momentum jump condition (capillary relationship of Pascal) at the liquid-vapor interface and geometrically relates the radius of curvature of the liquid meniscus to the volume fraction of vapor in the wick. The present model predicts the capillary limit and partial liquid recess (dryout) in the evaporator wick, and incorporates a liquid pooling submodel, which simulates accumulation of the excess liquid in the vapor core at the condenser end.

Tournier, Jean-Michel↗
NASA NTRS2010

Investigation of Loop Heat Pipe Survival and Restart After Extreme Cold Environment Exposure

NASA plans human exploration near the South Pole of the Moon, and other locations where the environment is extremely cold. This paper reports on the heat transfer performance of a loop heat pipe (LHP) exposed to extreme cold under the simulated reduced gravitational environment of the Moon. A common method of spacecraft thermal control is to use a LHP with ammonia working fluid. Typically, a small amount of heat is provided either by electrical heaters or by environmental design, such that the LHP condenser temperature never drops below the freezing point of ammonia. The concern is that a liquid-filled, frozen condenser would not restart, or that a thawing condenser would damage the tubing due to the expansion of ammonia upon thawing. This paper reports the results of an experimental investigation of a novel approach to avoid these problems. The LHP compensation chamber (CC) is conditioned such that all the ammonia liquid is removed from the condenser and the LHP is nonoperating. The condenser temperature is then reduced to below that of the ammonia freezing point. The LHP is then successfully restarted.

Golliher, Eric↗
DOE OSTI2021

A bioinspired and chemically defined alternative to dimethyl sulfoxide for the cryopreservation of human hematopoietic stem cells

Abstract The cryopreservation of hematopoietic cells using dimethyl sulfoxide (DMSO) and serum is a common procedure used in transplantation. However, DMSO has clinical and biological side effects due to its toxicity, and serum introduces variation and safety risks. Inspired by natural antifreeze proteins, a novel class of ice-interactive cryoprotectants was developed. The corresponding DMSO-, protein-, and serum-free cryopreservation media candidates were screened through a series of biological assays using human cell lines, peripheral blood cells, and bone marrow cells. XT-Thrive-A and XT-Thrive-B were identified as lead candidates to rival cryopreservation with 10% DMSO in serum based on post-thaw cell survival and short-term proliferation assays. The effectiveness of the novel cryopreservation media in freezing hematopoietic stem cells from human whole bone marrow was assessed by extreme limiting dilution analysis in immunodeficient mice. Stem cell frequencies were measured 12 weeks after transplant based on bone marrow engraftment of erythroid, myeloid, B-lymphoid, and CD34 + progenitors measured by flow cytometry. The recovered numbers of cryopreserved stem cells were similar among XT-Thrive A, XT-Thrive B, and DMSO with serum groups. These findings show that cryoprotectants developed through biomimicry of natural antifreeze proteins offers a substitute for DMSO-based media for the cryopreservation of hematopoietic stem cells.

60 APPLIED LIFE SCIENCES↗