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At least 235 records · Page 13

Gravity in mammalian organ development: differentiation of cultured lung and pancreas rudiments during spaceflight

Organ culture of embryonic mouse lung and pancreas rudiments has been used to investigate development and differentiation, and to assess the effects of microgravity on culture differentiation, during orbital spaceflight of the shuttle Endeavour (mission STS-54). Lung rudiments continue to grow and branch during spaceflight, an initial result that should allow future detailed study of lung morphogenesis in microgravity. Cultured embryonic pancreas undergoes characteristic exocrine acinar tissue and endocrine islet tissue differentiation during spaceflight, and in ground controls. The rudiments developing in the microgravity environment of spaceflight appear to grow larger than their ground counterparts, and they may have differentiated more rapidly than controls, as judged by exocrine zymogen granule presence.

STS-54 Shuttle Project↗

Meiosis, egg activation, and nuclear envelope breakdown are differentially reliant on Ca2+, whereas germinal vesicle breakdown is Ca2+ independent in the mouse oocyte

During early development, intracellular Ca2+ mobilization is not only essential for fertilization, but has also been implicated during other meiotic and mitotic events, such as germinal vesicle breakdown (GVBD) and nuclear envelope breakdown (NEBD). In this study, the roles of intracellular and extracellular Ca2+ were examined during meiotic maturation and reinitiation at parthenogenetic activation and during first mitosis in a single species using the same methodologies. Cumulus-free metaphase II mouse oocytes immediately resumed anaphase upon the induction of a large, transient Ca2+ elevation. This resumption of meiosis and associated events, such as cortical granule discharge, were not sensitive to extracellular Ca2+ removal, but were blocked by intracellular Ca2+ chelators. In contrast, meiosis I was dependent on external Ca2+; in its absence, the formation and function of the first meiotic spindle was delayed, the first polar body did not form and an interphase-like state was induced. GVBD was not dependent on external Ca2+ and showed no associated Ca2+ changes. NEBD at first mitosis in fertilized eggs, on the other hand, was frequently, but not always associated with a brief Ca2+ transient and was dependent on Ca2+ mobilization. We conclude that GVBD is Ca2+ independent, but that the dependence of NEBD on Ca2+ suggests regulation by more than one pathway. As cells develop from Ca(2+)-independent germinal vesicle oocytes to internal Ca(2+)-dependent pronuclear eggs, internal Ca2+ pools increase by approximately fourfold.

Non-NASA Center↗

Skeletal muscle fiber, nerve, and blood vessel breakdown in space-flown rats

Histochemical and ultrastructural analyses were performed postflight on hind limb skeletal muscles of rats orbited for 12.5 days aboard the unmanned Cosmos 1887 biosatellite and returned to Earth 2 days before sacrifice. The antigravity adductor longus (AL), soleus, and plantaris muscles atrophied more than the non-weight-bearing extensor digitorum longus, and slow muscle fibers were more atrophic than fast fibers. Muscle fiber segmental necrosis occurred selectively in the AL and soleus muscles; primarily, macrophages and neutrophils infiltrated and phagocytosed cellular debris. Granule-rich mast cells were diminished in flight AL muscles compared with controls, indicating the mast cell secretion contributed to interstitial tissue edema. Increased ubiquitination of disrupted myofibrils implicated ubiquitin in myofilament degradation. Mitochondrial content and succinic dehydrogenase activity were normal, except for subsarcolemmal decreases. Myofibrillar ATPase activity of flight AL muscle fibers shifted toward the fast type. Absence of capillaries and extravasation of red blood cells indicated failed microcirculation. Muscle fiber regeneration from activated satellite cells was detected. About 17% of the flight AL end plates exhibited total or partial denervation. Thus, skeletal muscle weakness associated with spaceflight can result from muscle fiber atrophy and segmental necrosis, partial motor denervation, and disruption of the microcirculation.

NASA Experiment Number COS 1887-6↗

Pancam multispectral imaging results from the Opportunity Rover at Meridiani Planum

Panoramic Camera (Pancam) images from Meridiani Planum reveal a low-albedo, generally flat, and relatively rock-free surface. Within and around impact craters and fractures, laminated outcrop rocks with higher albedo are observed. Fine-grained materials include dark sand, bright ferric iron-rich dust, angular rock clasts, and millimeter-size spheroidal granules that are eroding out of the laminated rocks. Spectra of sand, clasts, and one dark plains rock are consistent with mafic silicates such as pyroxene and olivine. Spectra of both the spherules and the laminated outcrop materials indicate the presence of crystalline ferric oxides or oxyhydroxides. Atmospheric observations show a steady decline in dust opacity during the mission. Astronomical observations captured solar transits by Phobos and Deimos and time-lapse observations of sunsets.

Mars↗

The Opportunity Rover's Athena science investigation at Meridiani Planum, Mars

The Mars Exploration Rover Opportunity has investigated the landing site in Eagle crater and the nearby plains within Meridiani Planum. The soils consist of fine-grained basaltic sand and a surface lag of hematite-rich spherules, spherule fragments, and other granules. Wind ripples are common. Underlying the thin soil layer, and exposed within small impact craters and troughs, are flat-lying sedimentary rocks. These rocks are finely laminated, are rich in sulfur, and contain abundant sulfate salts. Small-scale cross-lamination in some locations provides evidence for deposition in flowing liquid water. We interpret the rocks to be a mixture of chemical and siliciclastic sediments formed by episodic inundation by shallow surface water, followed by evaporation, exposure, and desiccation. Hematite-rich spherules are embedded in the rock and eroding from them. We interpret these spherules to be concretions formed by postdepositional diagenesis, again involving liquid water.

Mars↗

Validation of AIRS/AMSU Cloud Retrievals Using MODIS Cloud Analyses

The AIRS/AMSU (flying on the EOS-AQUA satellite) sounding retrieval methodology allows for the retrieval of key atmospheric/surface parameters under partially cloudy conditions (Susskind et al.). In addition, cloud parameters are also derived from the AIRS/AMSU observations. Within each AIRS footprint, cloud parameters at up to 2 cloud layers are determined with differing cloud top pressures and effective (product of infrared emissivity at 11 microns and physical cloud fraction) cloud fractions. However, so far the AIRS cloud product has not been rigorously evaluated/validated. Fortunately, collocated/coincident radiances measured by MODIS/AQUA (at a much lower spectral resolution but roughly an order of-magnitude higher spatial resolution than that of AIRS) are used to determine analogous cloud products from MODIS. This allows us for a rather rare and interesting possibility: the intercomparisons and mutual validation of imager vs. sounder-based cloud products obtained from the same satellite positions. First, we present results of small-scale (granules) instantaneous intercomparisons. Next, we will evaluate differences of temporally averaged (monthly) means as well as the representation of inter-annual variability of cloud parameters as presented by the two cloud data sets. In particular, we present statistical differences in the retrieved parameters of cloud fraction and cloud top pressure. We will investigate what type of cloud systems are retrieved most consistently (if any) with both retrieval schemes, and attempt to assess reasons behind statistically significant differences.

Molnar, Gyula I.↗

Environmental impacts on the developing CNS: CD15, NCAM-L1, and GFAP expression in rat neonates exposed to hypergravity

We have previously reported that the developing rat cerebellum is affected by hypergravity exposure. The effect is observed during a period of both granule and glial cell proliferation and neuronal migration in the cerebellum and coincides with changes in thyroid hormone levels. The present study begins to address the molecular mechanisms involved in the cerebellar response to hypergravity. Specifically, the study focuses on the expression of cerebellar proteins that are known to be directly involved in cell-cell interactions [protein expressing 3-fucosyl-N-acetyl-lactosamine antigen (CD15), neuronal cell adhesion molecule (NCAM-L1)] and those that affect cell-cell interactions indirectly [glial fibrillary acidic protein (GFAP)] in rat neonates exposed to centrifuge-produced hypergravity. Cerebellar mass and protein expression in rat neonates exposed to hypergravity (1.5 G) from gestational day (G) 11 to postnatal day (P) 30 were compared at one of six time points between P6 and P30 against rat neonates developing under normal gravity. Proteins were analyzed by quantitative western blots of cerebellar homogenates prepared from male or female neonates. Cerebellar size was most clearly reduced in male neonates on P6 and in female neonates on P9, with a significant gender difference; differences in cerebellar mass remained significant even when change in total body mass was factored in. Densitometric analysis of western blots revealed both quantitative and temporal changes in the expression of selected cerebellar proteins that coincided with changes in cerebellar mass and were gender-specific. In fact, our data indicated certain significant differences even between male and female control animals. A maximal decrease in expression of CD15 was observed in HG females on P9, coinciding with maximal change in their cerebellar mass. A shift in the time-course of NCAM-L1 expression resulted in a significant increase in NCAM-L1 in HG males on P18, an isolated time at which cerebellar mass does not significantly differ between HG and SC neonates. A maximal decrease in expression of GFAP was observed in HG males on P6, coinciding with maximal change in their cerebellar mass. Altered expression of cerebellar proteins is likely to affect a number of developmental processes and contribute to the structural and functional alterations seen in the CNS developing under altered gravity. Our data suggest that both cerebellar development and its response to gravitational manipulations differ in males and females. c2004 COSPAR. Published by Elsevier Ltd. All rights reserved.

NASA Program Fundamental Space Biology↗

Granular Materials and the Risks They Pose for Success on the Moon and Mars

Working with soil, sand, powders, ores, cement and sintered bricks, excavating, grading construction sites, driving off-road, transporting granules in chutes and pipes, sifting gravel, separating solids from gases, and using hoppers are so routine that it seems straightforward to do it on the Moon and Mars as we do it on Earth. This paper brings to the fore how little these processes are understood and the millennia-long trial-and-error practices that lead to today's massive over-design, high failure rate, and extensive incremental scaling up of industrial processes because of the inadequate predictive tools for design. We present a number of pragmatic scenarios where granular materials play a role, the risks involved, and what understanding is needed to greatly reduce the risks.

Wilkinson, R. Allen↗

Inter-calibrating Brightness Temperatures of a Constellation of Radiometers

In 2010, the National Aeronautics and Space Agency (NASA) of the U.S.A. and the Japanese Exploration Agency (JAXA) of Japan in cooperation with other U.S. and international partners will launch the Global Precipitation Measurement (GPM) mission. The mission center-piece is a core U.S. provided satellite holding a scanning microwave imager provided by the U.S. A. and a dual-frequency precipitation radar provided by Japan. The core satellite is in a 65 deg inclination (the current Tropical Rainfall Measuring Mission -TRMM is in a 35 deg inclination). Joining the core satellite are a constellation of approximately 8 satellites containing scanning radiometers. The purpose of the constellation is to increase the global sampling capability of the mission. One constellation satellite will be provided by NASA. Some of the constellation radiometers will provided by other U.S. agencies with existing (e.g. SSMI/S) or planned (NPOESS) radiometer assets. International groups have also expressed interest in contributing to the GPM mission including providing radiometer data for the GPM constellation. The use of a heterogeneous group of scanning radiometers each with its own unique purpose, characteristics and calibration offers a significant challenge for combining brightness temperatures or rain retrievals to create meaningful combined global radiometer products. However, the availability of active dual precipitation radar on the GPM core in combination with a well-calibrated radiometer on the same platform offers the possibility of inter-calibrating the constellation radiometers using the core satellite as a calibrator. This paper describes a joint NASA/GSFC and Colorado State University prototype effort at inter-calibrating existing radiometers using such a core calibrator approach. In the prototype, existing radiometers (i.e. SSM/I and AMSR-E) are intercalibrated, as required, using the TRMM Precipitation Radar (PR) and TRMM Microwave Imager (TMI) as the calibration core. This prototype includes four basic steps: provide the data in a normalized-common logical radiometer format, convert all data to a granule that stops and starts at the southernmost latitude rather than at the equator, perform basic processing and finally inter-calibrate the radiometers with the TRMM core as appropriate. Basic processing includes quality control, cross-track bias correction as well as the core action of converting T(sub a), to T(sub b). The purpose of the inter-calibration step is to provide consistent brightness temperatures for the various SSMA sensors, TMI and AMSR-E. The C appended to the level of the product indicates common brightness temperature (T(sub c)). The paper provides a summary of each of these actions. The details of the processing as well as data format and tools can be obtained by connecting to the website at mrain.atmos.colostate.eduLEVEL1C .

Stocker, Erich Franz↗

Imaging Spectroscopy of Sunspots using IBIS

We use the Interferometric BIdimensional Spectrometer (IBIS) of the INAF/Arcetri Astrophysical Observatory and installed at the National Solar Observatory (NSO) Dunn Solar Telescope, to understand the structure of sunspots. These high resolution observations were acquired on 2004 July 30-31, of active region NOAA 10654, using the high order NSO adaptive optics system. We map the spatio-temporal variation of the penumbral Doppler signatures in three spectral lines, FeI 6301.5\AA, FeII 7224.4\AA, and CaII 8542.6\AA, from the photosphere to the chromosphere. From a 70-minute temporal average of individual 32-second cadence Doppler observations we find that the averaged velocities decrease with height, about 3.5 times larger in the deeper photosphere (FeII 7224.4\AA; height-of-formation $\approx$\50 km) than in the upper photosphere FeI 6301.5\AA; height-of-formation $\approx$\350 km), There is a remarkable coherence of Doppler signals over the height difference of 300 km. From a highspeed animation of the Doppler sequence we find evidence for what appears to be ejection of high speed gas concentrations from edges of penumbral filaments into the surrounding granular photosphere. The Evershed flow persists a few arcseconds beyond the traditionally demarcated penumbra-granulation boundary. We present these and other results and discuss the implications of these measurements for sunspot models

Balasubramaniam, K. S.↗

ECHO Status for International Partners

The EOS Clearinghouse (ECHO) is a clearinghouse of spatial and temporal metadata, inclusive of NASA's Distributed Active Archive Center (DAAC) data holdings, that enables the science community to more easily exchange NASA data and information. Currently, ECHO has metadata descriptors for over 55 million individual data granules and 13 million browse images. The majority of ECHO's holdings come directly from data held in the NASA DAACs. The science disciplines and domains represented in ECHO are diverse and include metadata for all of NASA's Science Focus Area data. As middleware for a service-oriented enterprise, ECHO offers access to its capabilities through a set of publicly available Application Program Interfaces (APIs). More information about ECHO is available at http://eos.nasa.gov.echo. The presentation will discuss the status of the ECHO Partners, holdings, and activities, including the transition from the EOS Data Gateway to the Warehouse Inventory Search Tool (WIST)

Weinstein, Beth↗

Control of a simulated arm using a novel combination of Cerebellar learning mechanisms

We present a model of cerebellar cortex that combines two types of learning: feedforward predicitve association based on local Hebbian-type learning between granule cell ascending branch and parallel fiber inputs, and reinforcement learning with feedback error correction based on climbing fiber activity.

cerebellum cerebellar learning dynamic state estim↗

Improved Thin Cirrus and Terminator Cloud Detection in CERES Cloud Mask

Thin cirrus clouds account for about 20-30% of the total cloud coverage and affect the global radiation budget by increasing the Earth's albedo and reducing infrared emissions. Thin cirrus, however, are often underestimated by traditional satellite cloud detection algorithms. This difficulty is caused by the lack of spectral contrast between optically thin cirrus and the surface in techniques that use visible (0.65 micron ) and infrared (11 micron ) channels. In the Clouds and the Earth s Radiant Energy System (CERES) Aqua Edition 1 (AEd1) and Terra Edition 3 (TEd3) Cloud Masks, thin cirrus detection is significantly improved over both land and ocean using a technique that combines MODIS high-resolution measurements from the 1.38 and 11 micron channels and brightness temperature differences (BTDs) of 11-12, 8.5-11, and 3.7-11 micron channels. To account for humidity and view angle dependencies, empirical relationships were derived with observations from the 1.38 micron reflectance and the 11-12 and 8.5-11 micron BTDs using 70 granules of MODIS data in 2002 and 2003. Another challenge in global cloud detection algorithms occurs near the day/night terminator where information from the visible 0.65 micron channel and the estimated solar component of 3.7 micron channel becomes less reliable. As a result, clouds are often underestimated or misidentified near the terminator over land and ocean. Comparisons between the CLAVR-x (Clouds from Advanced Very High Resolution Radiometer [AVHRR]) cloud coverage and Geoscience Laser Altimeter System (GLAS) measurements north of 60 N indicate significant amounts of missing clouds from CLAVR-x because this part of the world was near the day/night terminator viewed by AVHRR. Comparisons between MODIS cloud products (MOD06) and GLAS in the same region also show similar difficulties with MODIS cloud retrievals. The consistent detection of clouds through out the day is needed to provide reliable cloud and radiation products for CERES and other research efforts involving the modeling of clouds and their interaction with the radiation budget.

Trepte, Qing↗

3-D Structure of Sunspots using Imaging Spectroscopy

We use the Interferometric BIdimensional Spectrometer (IBIS) of the INAF/Arcetri Astrophysical Observatory and installed at the National Solar Observatory (NSO) Dunn Solar Telescope, to understand the structure of sunspots. Using the spectral lines FeI 6301.5 A, FeII 7224.4 A, and CaII 8542.6 A, we examine the spectroscopic variation of sunspot penumbral and umbral structures at the heights of formation of these lines. These high resolution observations were acquired on 2004 July 30-31, of active region NOAA 10654, using the high order NSO adaptive optics system. We map the spatio-temporal variation of Doppler signatures in these spectral lines, from the photosphere to the chromosphere. From a 70-minute temporal average of individual 32-second cadence Doppler observations we find that the averaged velocities decrease with height, about 3.5 times larger in the deeper photosphere (FeII 7224.4 A; height-of-formation approx. 50 km) than in the upper photosphere FeI 6301.5 A; height-of-formation approx. 350 km), There is a remarkable coherence of Doppler signals over the height difference of 300 km. From a high-speed animation of the Doppler sequence we find evidence for what appears to be ejection of high speed gas concentrations from edges of penumbral filaments into the surrounding granular photosphere. The Evershed flow persists a few arcseconds beyond the traditionally demarcated penumbra-granulation boundary. We present these and other results and discuss the implications of these measurements for sunspot models.

Balasubramaniam, K. S.↗

Chemical Composition by the APXS along the Downhill Traverse of the Mars Exploration Rover Spirit at Gusev Crater

The Alpha Particle X-ray Spectrometer (APXS) onboard the Mars Exploration Rover Spirit continues to determine the elemental composition of samples at Gusev Crater. Starting around sol 600 the rover descended Husband Hill, which is part of the Columbia Hills, visited the inner basin with a large dune field, called 'El Dorado', and parked at Low Ridge to conserve energy during the martian winter. Many unique samples were discovered by the instruments onboard Spirit during her downhill traverse. Here, we report only on the chemical data obtained by the APXS. The compositions of some of the soil samples are comparable to the mean soil determined along the earlier traverse. However, a light-toned subsurface sample (disturbed by the rover wheels), called Dead Sea Samra , showed the highest sulfur content of all soil samples, the lowest Na, Mg, Al, Cl, K, Ca, Ti, Mn, and Zn, among the lowest Si and P, and among the highest Cr, Fe and Ni. Assuming ferric sulfate as a major mineral, large amounts of a pure silica phase must be present. Color and quantity of Dead Sea Samra resemble somewhat an earlier soil called Paso Robles , though the latter is a mixture of sulfates with phosphate-rich soil. Manganese in Dead Sea Samra is so low that the Fe/Mn ratio exceeds 300, a value that has never been found previously on Mars (Fe/Mn ratio of 46 for Gusev basalts), indicating that only Fe(3+) occurs. The dune field El Dorado contained granulated material that exhibited the highest Mg and Ni concentrations and the lowest S and Cl compared to all other soils implying an enrichment of olivine-rich sands. Two outcrops, called Algonquin and Comanche , revealed compositions that differ significantly from those of earlier outcrops as they have the highest concentrations of Mg, Fe, and Ni (except for Ni in Independence) and the lowest of Al, K (detection limit), Ca, and Ti of all brushed and almost all abraded rocks. Normative estimates assign them the highest olivine contents ever found for martian rocks and a very mafic nature based on their high Mg/(Mg+Fe) and low Al, Ca and Na. Their significantly high Ni contents point to a different source than the Gusev plains basalts. The elemental compositions of samples encountered during the downhill traverse revealed a larger chemical diversity of the Columbia Hills than the uphill trek already published.

Bruckner, J.↗

Evaluation of AIRS, MODIS, and HIRS 11 Micron Brightness Temperature Difference Changes from 2002 through 2006

In an effort to validate the accuracy and stability of AIRS data at low scene temperatures (200-250 K range), we evaluated brightness temperatures at 11 microns with Aqua MODIS band 31 and HIRS/3 channel 8 for Antarctic granules between September 2002 and May 2006. We found excellent agreement with MODIS (at the 0.2 K level) over the full emperature range in data from early in the Aqua mission. However, in more recent data, starting in April 2005, we found a scene temperature dependence in MODIS-AIRS brightness temperature differences, with a discrepancy of 1- 1.5 K at 200 K. The comparison between AIRS and HIRS/3 (channel 8) on NOAA 16 for the same time period yields excellent agreement. The cause and time dependence of the disagreement with MODIS is under evaluation, but the change was coincident with a change in the MODIS production software from collection 4 to 5.

MODIS↗

A New Algorithm for Retrieving Aerosol Properties Over Land from MODIS Spectral Reflectance

Since first light in early 2000, operational global quantitative retrievals of aerosol properties over land have been made from MODIS observed spectral reflectance. These products have been continuously evaluated and validated, and opportunities for improvements have been noted. We have replaced the original algorithm by improving surface reflectance assumptions, the aerosol model optical properties and the radiative transfer code used to create the lookup tables. The new algorithm (known as Version 5.2 or V5.2) performs a simultaneous inversion of two visible (0.47 and 0.66 micron) and one shortwave-IR (2.12 micron) channel, making use of the coarse aerosol information content contained in the 2.12 micron channel. Inversion of the three channels yields three nearly independent parameters, the aerosol optical depth (tau) at 0.55 micron, the non-dust or fine weighting (eta) and the surface reflectance at 2.12 micron. Finally, retrievals of small magnitude negative tau values (down to -0.05) are considered valid, thus normalizing the statistics of tau in near zero tau conditions. On a 'test bed' of 6300 granules from Terra and Aqua, the products from V5.2 show marked improvement over those from the previous versions, including much improved retrievals of tau, where the MODIS/AERONET tau (at 0.55 micron) regression has an equation of: y = 1.01+0.03, R = 0.90. Mean tau for the test bed is reduced from 0.28 to 0.21.

Levy, Robert C.↗

Voltage Controller Saves Energy, Prolongs Life of Motors

In 1985, Power Efficiency Corporation of Las Vegas licensed NASA voltage controller technology from Marshall Space Flight Center. In the following years, Power Efficiency made patented improvements to the technology and marketed the resulting products throughout the world as the Performance Controller and the Power Efficiency energy-saving soft start. Soft start gradually introduces power to an electric motor, thus eliminating the harsh, violent mechanical stresses of having the device go from a dormant state to one of full activity; prevents it from running too hot; and increases the motor's lifetime. The product can pay for itself through the reduction in electricity consumed (according to Power Efficiency, within 3 years), depending on the duty cycle of the motor and the prevailing power rates. In many instances, the purchaser is eligible for special utility rebates for the environmental protection it provides. Common applications of Power Efficiency's soft start include mixers, grinders, granulators, conveyors, crushers, stamping presses, injection molders, elevators with MG sets, and escalators. The device has been retrofitted onto equipment at major department store chains, hotels, airports, universities, and for various manufacturers

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