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At least 235 records · Page 13

Effect of hydrogen isotope on plasma impedance and thermal pinching induced by single aerosol droplets injected into an inductively coupled plasma

The mass of the hydrogen isotope in micrometer-sized aqueous droplets introduced into an ICP has been found to affect the degree of resulting plasma perturbation. Studied plasma perturbations include local plasma cooling, plasma shrinkage due to thermal pinching, and changes in plasma impedance. The local cooling effect caused by a vaporizing aerosol droplet was found to be similar between H 2 O and D 2 O. This finding is likely a result of the similar heat capacities and vaporization rates of H 2 O and D 2 O droplets and of the comparable thermal conductivities and dissociation kinetics of H 2 O and D 2 O vapor. In contrast, a clear isotope effect was observed for plasma shrinkage and impedance change, with H 2 O causing a considerably stronger effect. Plasma shrinkage, gauged by the reduction in Ar emission at the edge of the plasma, was detected at all observation heights within the load-coil region, with D 2 O droplets causing as much as 30% less of an effect than H 2 O. Likewise, D 2 O droplets induce significantly less change in plasma impedance than H 2 O droplets. Depending on experimental conditions and the probing method, changes in plasma impedance caused by monodisperse D 2 O droplets were only 52%–80% that of H 2 O. The lower influence on plasma shrinkage and impedance change for D 2 O droplets is believed to be related to the much lower thermal conductivity of atomic deuterium, around 70% that of atomic hydrogen (protium). The time required for the plasma impedance to return to its original steady-state value was comparatively long (~10 ms) and similar for H 2 O and D 2 O droplets.

70 PLASMA PHYSICS AND FUSION TECHNOLOGY↗

Observation of second harmonic electron cyclotron resonance heating and current-drive transition during non-inductive plasma start-up experiment in QUEST

Here, noninductive plasma current start-up using 2nd harmonic electron cyclotron resonance heating (ECRH) with oblique radio frequency (RF) injection is demonstrated in a Q-shu University experiment with steady-state spherical tokamak. A strong transition was observed in the heating and plasma current ramp-up. The initial bulk electron heating regime exhibits T ebulk ~ 140 eV and no hard x-ray (HXR) emission with a low I p of ~15 kA; it abruptly transitions to a regime that exhibits a low T ebulk of ~10 eV and a strong HXR emission with a high I p of ~50 kA. This behavior is distinctly different from that observed in previous fundamental ECRH experiments. The mechanism of the heating and current drive transition are investigated considering wave power absorption and plasma power balance. The results indicate that the transition is caused by the favorable heating of tail electrons where the RF power absorption at the 2nd harmonic increases nearly linearly with T etail , while the power transfer from the tail electrons to the bulk electrons decreases with 1/T etail 0.5 . This causes a rapid transition to a state with high T etail while reducing T ebulk towards colder ion temperature. The understanding of the transition mechanism helps to consider plasma current start-up using 2nd harmonic ECRH for tokamak reactors such as JT-60 SA and ITER.

QUEST↗

Balancing Doses of EL222 and Light Improves Optogenetic Induction of Protein Production in Komagataella phaffii

ABSTRACT Komagataella phaffii, also known asPichia pastoris, is a powerful host for recombinant protein production, in part due to its exceptionally strong and tightly controlled P AOX1 promoter. MostK. phaffiibioprocesses for recombinant protein production rely on P AOX1 to achieve dynamic control in two‐phase processes. Cells are first grown under conditions that repress P AOX1 (growth phase), followed by methanol‐induced recombinant protein expression (production phase). In this study, we propose a methanol‐free approach for dynamic metabolic control inK. phaffiiusing optogenetics, which can help enhance input tunability and flexibility in process optimization and control. The light‐responsive transcription factor EL222 fromErythrobacter litoralisis used to regulate protein production from the P C120 promoter inK. phaffiiwith blue light. We used two system designs to explore the advantages and disadvantages of coupling or decoupling EL222 integration with that of the gene of interest. We investigate the relationship between EL222 gene copy number and light dosage to improve production efficiency for intracellular and secreted proteins. Experiments in lab‐scale bioreactors demonstrate the feasibility of the outlined optogenetic systems as potential alternatives to conventional methanol‐inducible bioprocesses usingK. phaffii.

Biotechnology & Applied Microbiology↗

Machining-inducted residual strain and ductile-brittle transition in sapphire revealed by X-ray nanoprobe diffraction microscopy

Hard X-ray nanoprobe diffraction microscopy is employed to investigate machining-induced residual lattice strain in single-crystal sapphire following ultra-precision orthogonal plunge cutting. In this study, tilt-series and single-angle acquisition strategies are systematically compared to evaluate their capability for resolving lattice variations associated with the machining process. An analytical framework is developed to enable the efficient and reliable extraction of strains, which serve as strain-based indicators of changes in the dominant deformation mechanism. The measured high-dimensional strain exhibits spatial heterogeneity and systematic evolution along the cutting path, consistent with deformation-regime changes from ductile response to crack-prone behavior.

Liang, Rui↗