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DOE OSTI2022

Wavefront shaping with a Hadamard basis for scattering soil imaging

Here, soil is a scattering medium that inhibits imaging of plant-microbial-mineral interactions that are essential to plant health and soil carbon sequestration. However, optical imaging in the complex medium of soil has been stymied by the seemingly intractable problems of scattering and contrast. Here, we develop a wavefront shaping method based on adaptive stochastic parallel gradient descent optimization with a Hadamard basis to focus light through soil mineral samples. Our approach allows a sparse representation of the wavefront with reduced dimensionality for the optimization. We further divide the used Hadamard basis set into subsets and optimize a certain subset at once. Simulation and experimental optimization results demonstrate our method has an approximately seven times higher convergence rate and overall better performance compared to that with optimizing all pixels at once. The proposed method can benefit other high-dimensional optimization problems in adaptive optics and wavefront shaping.

47 OTHER INSTRUMENTATION↗

NASA NTRS1991

Light as a chronobiologic countermeasure for long-duration space operations

Long-duration space missions require adaptation to work-rest schedules which are substantially shifted with respect to earth. Astronauts are expected to work in two-shift operations and the environmental synchronizers (zeitgebers) in a spacecraft differ significantly from those on earth. A study on circadian rhythms, sleep, and performance was conducted by exposing four subjects to 6 deg head-down tilt bedrest (to simulate the effects of the weightless condition) and imposing a 12-h shift (6 h delay per day for two days). Bright light was tested in a cross-over design as a countermeasure for achieving faster resynchronization and regaining stable conditions for sleep and circadian rhythmicity. Data collection included objective sleep recording, temperature, heart rate, and excretion of hormones and electrolytes as well as performance and responses to questionnaires. Even without a shift in the sleep-wake cycle, the sleep quantity, circadian amplitudes and 24 h means decreased in many functions under bedrest conditions. During the shift days, sleepiness and fatigue increased, and alertness decreased. However, sleep quantity was regained, and resynchronization was completed within seven days after the shift for almost all functions, irrespective of whether light was administered during day-time or night-time hours. The time of day of light exposure surprisingly appeared not to have a discriminatory effect on the resynchronization speed under shift and bedrest conditions. The results indicate that simulated weightlessness alters circadian rhythms and sleep, and that schedule changes induce additional physiological disruption with decreased subjective alertness and increased fatigue. Because of their operational implications, these phenomena deserve additional investigation.

Samel, Alexander↗

DOE OSTI2020

Linking the Dynamic Response of the Carbon Dioxide-Concentrating Mechanism to Carbon Assimilation Behavior in Fremyella diplosiphon

ABSTRACT Cyanobacteria use a carbon dioxide (CO 2 )-concentrating mechanism (CCM) that enhances their carbon fixation efficiency and is regulated by many environmental factors that impact photosynthesis, including carbon availability, light levels, and nutrient access. Efforts to connect the regulation of the CCM by these factors to functional effects on carbon assimilation rates have been complicated by the aqueous nature of cyanobacteria. Here, we describe the use of cyanobacteria in a semiwet state on glass fiber filtration discs—cyanobacterial discs—to establish dynamic carbon assimilation behavior using gas exchange analysis. In combination with quantitative PCR (qPCR) and transmission electron microscopy (TEM) analyses, we linked the regulation of CCM components to corresponding carbon assimilation behavior in the freshwater, filamentous cyanobacterium Fremyella diplosiphon . Inorganic carbon (C i ) levels, light quantity, and light quality have all been shown to influence carbon assimilation behavior in F. diplosiphon . Our results suggest a biphasic model of cyanobacterial carbon fixation. While behavior at low levels of CO 2 is driven mainly by the C i uptake ability of the cyanobacterium, at higher CO 2 levels, carbon assimilation behavior is multifaceted and depends on C i availability, carboxysome morphology, linear electron flow, and cell shape. Carbon response curves (CRCs) generated via gas exchange analysis enable rapid examination of CO 2 assimilation behavior in cyanobacteria and can be used for cells grown under distinct conditions to provide insight into how CO 2 assimilation correlates with the regulation of critical cellular functions, such as the environmental control of the CCM and downstream photosynthetic capacity. IMPORTANCE Environmental regulation of photosynthesis in cyanobacteria enhances organismal fitness, light capture, and associated carbon fixation under dynamic conditions. Concentration of carbon dioxide (CO 2 ) near the carbon-fixing enzyme RubisCO occurs via the CO 2 -concentrating mechanism (CCM). The CCM is also tuned in response to carbon availability, light quality or levels, or nutrient access—cues that also impact photosynthesis. We adapted dynamic gas exchange methods generally used with plants to investigate environmental regulation of the CCM and carbon fixation capacity using glass fiber-filtered cells of the cyanobacterium Fremyella diplosiphon . We describe a breakthrough in measuring real-time carbon uptake and associated assimilation capacity for cells grown in distinct conditions (i.e., light quality, light quantity, or carbon status). These measurements demonstrate that the CCM modulates carbon uptake and assimilation under low-C i conditions and that light-dependent regulation of pigmentation, cell shape, and downstream stages of carbon fixation are critical for tuning carbon uptake and assimilation.

59 BASIC BIOLOGICAL SCIENCES↗

DOE OSTI2025

Identification of Important Phenomena for Light Water Reactors During Heat Transport System Failure Events in Integrated Energy Systems

This work adapts historical literature and existing phenomena identification and ranking tables (PIRT) to be applicable to a novel nuclear power plant (NPP) and chemical or thermal process integrated energy system (IES), particularly focusing on the process heat and heat transport system failure events that are not a concern during normal NPP operation but become vital when an IES is considered. Nuclear energy has been suggested to go beyond base-load applications and be used for hydrogen co-generation systems, amongst other IESs. Prior to the implementation of nuclear IESs, sufficient analysis must be performed on accident events to ensure public safety. The events considered were deemed important because of their potential to damage systems, structures, and components (SSCs). Process thermal events of concern include loss of heat load and temperature transient events. Loss of heat load events were characterized as having high importance and being well understood. Temperature transient events may be further categorized by the cyclic loading and harmonics phenomena. Cyclic loading issues were classified as medium to high importance with knowledge gaps existing regarding fatigue and low power operation, while harmonics phenomena were classified as low importance and are well understood. Heat transport system failure events of concern include intermediate and process heat exchanger failures, mass addition to reactor coolant, ingress of material from thermal manifold/energy storage, and loss of intermediate fluid. Furthermore, these events tended to be of high or medium importance, with some knowledge gaps needing to be filled for individual reactor systems due to unique designs.

Integrated Energy System (IES)↗

NASA NTRS1993

Direct 75 milliarcsecond images from the Multiple Mirror Telescope with adaptive optics

We report results from an adaptive optics system designed to provide imaging at the diffraction limit of resolution in the near-infrared at the Multiple Mirror Telescope (MMT). For the present experiment, the aperture consisted of five of the six primary mirrors of the MMT, operating as a coherently phased array. The largest components of the atmospherically induced wave-front aberration are the fluctuations in mean phase between the segments. These errors were derived in real time from the Fourier transform of short-exposure stellar images at 2.2 microns and corrected at an image of the telescope pupil with piston motion from a segmented adaptive mirror. At a correction rate of 43 Hz, this level of adaptive control resulted in an integrated image with a clear diffraction-limited component of 0.075 arcsec FWHM. This stabilized component is present directly in the light arriving at the detector and is not the result of postprocessing. We discuss future improvements to our adaptive wave-front control and its application to astronomical observations.

Lloyd-Hart, M.↗

NASA NTRS2018

The NEID Precision Radial Velocity Spectrometer: Port Adapter Overview, Requirements, and Test Plan

The NEID spectrometer is an optical (380-930 nm), fiber-fed, precision Doppler spectrometer currently in development for the WIYN 3.5 m telescope at Kitt Peak National Observatory as part of the NN-EXPLORE partnership. Designed to achieve a radial velocity precision of < 30 cm/s, NEID will be sensitive enough to detect terrestrial-mass exoplanets around low-mass stars. Light from the target stars is focused by the telescope to a bent Cassegrain port at the edge of the primary mirror mechanical support. The specialized NEID "Port Adapter" system is mounted at this bent Cassegrain port and is responsible for delivering the incident light from the telescope to the NEID fibers. In order to provide stable, high-quality images to the science instrument, the Port Adapter houses several sub-components designed to acquire the target stars, correct for atmospheric dispersion, stabilize the light onto the science fibers, and calibrate the spectrometer by injecting known wavelength sources such as a laser frequency comb. Here we provide an overview of the overall opto-mechanical design and system requirements of the Port Adapter. We also describe the development of system error budgets and testplans to meet those requirements.

Cassegrain port↗

DOE OSTI2024

CIRCLES: Congestion Impacts Reduction via CAV-in-the-loop Lagrangian Energy Smoothing

The energy efficiency of today’s vehicular mobility relies on the un-integrated combination of i) control via static assets (traffic lights, metering, variable speed limits, etc.); and ii) onboard vehicle automation (adaptive cruise control (ACC), ecodriving, etc.). These two families of control were not co-designed and are not engineered to work in coordination. Recent studies have shown i) limitations of controls, and even ii) negative impacts of ACC. This project focused on the technology development, implementation and prototyping, and validation of Mobile Traffic Control (MTC). MTC can be viewed as an extension of classical traffic control (in which static infrastructure actuates traffic flow). In the MTC paradigm, automated vehicles actuate the entire flow via their behavior, offering enhanced possibilities to optimize the energy footprint of traffic, if designed correctly. We set out to demonstrate for the first time that considerably reduced fuel consumption of all vehicles in traffic can be achieved via distributed control of a small proportion of CAVs. Compared to baseline vehicular technologies, our work offers a significant design departure: control algorithms for the CAVs consider the impact one vehicle can have on overall traffic, improving resulting overall fuel consumption. We focus on using a few vehicles (as traffic controllers via CAV technology) to improve the energy efficiency of traffic flow to further optimize energy efficiency. A live-traffic demonstration in November 2022 featured the deployment of 100 specially-equipped CAVs, constituting an approximate local penetration rate upwards of 2%.

32 ENERGY CONSERVATION, CONSUMPTION, AND UTILIZATI↗

NASA NTRS1976

Effect of color on pilot performance and transfer functions using a full-spectrum, calligraphic, color display system

The use of blue and red color in out-of-window cockpit displays, in full-spectrum calligraphic computer-generated display systems, is studied with attention given to pilot stereographic depth perception and response to visual cues. Displays for vertical approach, with dynamic and frozen-range landing approach and perspective arrays, are analyzed. Pilot transfer function and the transfer function associated with the contrasted approach and perspective arrays are discussed. Out-of-window blue lights are perceived by pilots as indicating greater distance depth, red lights as indicating proximity. The computer-generated chromatic display was adapted to flight simulators for the tests.

Chase, W. D.↗

NASA NTRS1992

On pose determination using point features

Consideration is given to the vision subsystem of an Orbital Replacement Unit (ORU) that was placed onto its base at Goddard Space Flight Center by a PUMA 762 robot equipped with a wrist-mounted CCD camera and a wrist-mounted force sensor. It is found that a simple adaptive thresholding method works quite well for images taken under various lighting conditions. The pose computed using the quadrangle method is reasonable for real images. In the presence of image feature noise the accuracy of the computed pose can be considerably reduced. This problem can be solved by using a 3D marker and an alternative pose computation algorithm.

Hwang, Vincent↗

NASA NTRS1995

Schlieren optics for leak detection

The purpose of this research was to develop an optical method of leak detection. Various modifications of schlieren optics were explored with initial emphasis on leak detection of the plumbing within the orbital maneuvering system of the space shuttle (OMS pod). The schlieren scheme envisioned for OMS pod leak detection was that of a high contrast pattern on flexible reflecting material imaged onto a negative of the same pattern. We find that the OMS pod geometry constrains the characteristic length scale of the pattern to the order of 0.001 inch. Our experiments suggest that optical modulation transfer efficiency will be very low for such patterns, which will limit the sensitivity of the technique. Optical elements which allow a negative of the scene to be reversibly recorded using light from the scene itself were explored for their potential in adaptive single-ended schlieren systems. Elements studied include photochromic glass, bacteriorhodopsin, and a transmissive liquid crystal display. The dynamics of writing and reading patterns were studied using intensity profiles from recorded images. Schlieren detection of index gradients in air was demonstrated.

Peale, Robert E.↗

NASA NTRS2013

Hyperspectral Imager-Tracker

The Hyperspectral Imager-Tracker (HIT) is a technique for visualization and tracking of low-contrast, fast-moving objects. The HIT architecture is based on an innovative and only recently developed concept in imaging optics. This innovative architecture will give the Light Prescriptions Innovators (LPI) HIT the possibility of simultaneously collecting the spectral band images (hyperspectral cube), IR images, and to operate with high-light-gathering power and high magnification for multiple fast- moving objects. Adaptive Spectral Filtering algorithms will efficiently increase the contrast of low-contrast scenes. The most hazardous parts of a space mission are the first stage of a launch and the last 10 kilometers of the landing trajectory. In general, a close watch on spacecraft operation is required at distances up to 70 km. Tracking at such distances is usually associated with the use of radar, but its milliradian angular resolution translates to 100- m spatial resolution at 70-km distance. With sufficient power, radar can track a spacecraft as a whole object, but will not provide detail in the case of an accident, particularly for small debris in the onemeter range, which can only be achieved optically. It will be important to track the debris, which could disintegrate further into more debris, all the way to the ground. Such fragmentation could cause ballistic predictions, based on observations using high-resolution but narrow-field optics for only the first few seconds of the event, to be inaccurate. No optical imager architecture exists to satisfy NASA requirements. The HIT was developed for space vehicle tracking, in-flight inspection, and in the case of an accident, a detailed recording of the event. The system is a combination of five subsystems: (1) a roving fovea telescope with a wide 30 field of regard; (2) narrow, high-resolution fovea field optics; (3) a Coude optics system for telescope output beam stabilization; (4) a hyperspectral-mutispectral imaging assembly; and (5) image analysis software with effective adaptive spectral filtering algorithm for real-time contrast enhancement.

Agurok, Llya↗

NASA NTRS1991

Characterization of adaptation in phototropism of Arabidopsis thaliana

Phototropic curvature has been measured for etiolated Arabidopsis thaliana seedlings with and without a preirradiation. A bilateral preirradiation with 450-nm light at a fluence greater than about 0.1 micromole per square meter causes a rapid desensitization to a subsequent 450-nanometer unilateral irradiation at 0.5 micromole per square meter. Following a refractory period, the capacity to respond phototropically recovers to the predesensitization level, and the response is then enhanced. The length of the refractory period is between 10 and 20 minutes. Both the time needed for recovery and the extent of enhancement increase with increasing fluence of the bilateral preirradiation. Based on the relative spectral sensitivities of desensitization and enhancement, these responses can be separated. Desensitization is induced by blue light but not by red light. Enhancement, however, is induced by both blue and red light. Thus, enhancement can be induced without desensitization but not vice versa. Both desensitization and enhancement affect only the magnitude of the response and do not affect the fluence threshold.

NASA Program Space Biology↗

NASA NTRS1996

The Physiology of Bed Rest

Prolonged rest in bed has been utilized by physicians and other health-care workers to immobilize and confine patients for rehabilitation and restoration of health since time immemorial. The sitting or horizontal position is sought by the body to relieve the strain of the upright or vertical postures, for example during syncopal situations, bone fractures, muscle injuries, fatigue, and probably also to reduce energy expenditure. Most health-care personnel are aware that adaptive responses occurring during bed rest proceed concomitantly with the healing process; signs and symptoms associated with the former should be differentiated from those of the latter. Not all illnesses and infirmities benefit from prolonged bed rest. Considerations in prescribing bed rest for patients-including duration, body position, mode and duration of exercise, light-dark cycles, temperature, and humidity-have not been investigated adequately. More recently, adaptive physiological responses have been measured in normal, healthy subjects in the horizontal or slightly head-down postures during prolonged bed rest as analogs for the adaptive responses of astronauts exposed to the microgravity environment of outer and bed-rest research.

Fortney, Suzanne M.↗

NASA NTRS1985

A study of topologies and protocols for fiber optic local area network

The emergence of new applications requiring high data traffic necessitates the development of high speed local area networks. Optical fiber is selected as the transmission medium due to its inherent advantages over other possible media and the dual optical bus architecture is shown to be the most suitable topology. Asynchronous access protocols, including token, random, hybrid random/token, and virtual token schemes, are developed and analyzed. Exact expressions for insertion delay and utilization at light and heavy load are derived, and intermediate load behavior is investigated by simulation. A new tokenless adaptive scheme whose control depends only on the detection of activity on the channel is shown to outperform round-robin schemes under uneven loads and multipacket traffic and to perform optimally at light load. An approximate solution to the queueing delay for an oscillating polling scheme under chaining is obtained and results are compared with simulation. Solutions to the problem of building systems with a large number of stations are presented, including maximization of the number of optical couplers, and the use of passive star/bus topologies, bridges and gateways.

Yeh, C.↗

NASA NTRS2024

Sensitivity studies of nighttime top-of-atmosphere radiances from artificial light sources using a 3-D radiative transfer model for nighttime aerosol retrievals

By accounting for surface-based light source emissions and top-of-atmosphere (TOA) downward lunar fluxes, we adapted the spherical harmonics discrete ordinate method (SHDOM) 3-dimensional (3-D) radiative transfer model (RTM) to simulate nighttime 3-D TOA radiances as observed from the Visible Infrared Imaging Radiometer Suite (VIIRS) Day/Night Band (DNB) on board the Suomi-NPP satellite platform. Used previously for daytime 3-D applications, these new SHDOM enhancements allow for the study of the impacts of various observing conditions and aerosol properties on simulated VIIRS-DNB TOA radiances. Observations over Dakar, Senegal, selected for its bright city lights and a large range of aerosol optical depth (AOD), were investigated for potential applications and opportunities for using observed radiances containing VIIRS-DNB “bright pixels” from artificial light sources to conduct aerosol retrievals. We found that using the standard deviation (SD) of such bright pixels provided a more stable quantity for nighttime AOD retrievals than direct retrievals from TOA radiances. Further, both the mean TOA radiance and SD of TOA radiances over artificial sources are significantly impacted by satellite viewing angles. Light domes, the enhanced radiances adjacent to artificial light sources, are strong functions of aerosol properties and especially aerosol vertical distribution, which may be further utilized for retrieving aerosol layer height in future studies. Through inter-comparison with both day- and nighttime Aerosol Robotic Network (AERONET) data, the feasibility of retrieving nighttime AODs using 3-D RTM SHDOM over artificial light sources was demonstrated. Our study shows strong potential for using artificial light sources for nighttime AOD retrievals, while also highlighting larger uncertainties in quantifying surface light source emissions. This study underscores the need for surface light emission source characterizations as a key boundary condition, which is a complex task that requires enhanced input data and further research. We demonstrate how quality-controlled nighttime light data from NASA’s Black Marble product suite could serve as a primary input into estimations of surface light source emissions for nighttime aerosol retrievals.

Jianglong Zhang↗

NASA NTRS2009

Spatial Cognitive Performance During Adaptation to Conflicting Tilt-Translation Stimuli as a Sensorimotor Spaceflight Analog

The need to resolve new patterns of sensory feedback in altered gravitoinertial environments requires cognitive processes to develop appropriate reference frames for spatial orientation awareness. The purpose of this study was to examine deficits in spatial cognitive performance during adaptation to conflicting tilt-translation stimuli. Fourteen subjects were tilted within a lighted enclosure that simultaneously translated at one of 3 frequencies. Tilt and translation motion was synchronized to maintain the resultant gravitoinertial force aligned with the longitudinal body axis, resulting in a mismatch analogous to spaceflight in which the canals and vision signal tilt while the otoliths do not. Changes in performance on different spatial cognitive tasks were compared 1) without motion, 2) with tilt motion alone (pitch at 0.15, 0.3 and 0.6 Hz or roll at 0.3 Hz), and 3) with conflicting tilt-translation motion. The adaptation paradigm was continued for up to 30 min or until the onset of nausea. The order of the adaptation conditions were counter-balanced across 4 different test sessions. There was a significant effect of stimulus frequency on both motion sickness and spatial cognitive performance. Only 3 of 14 were able to complete the full 30 min protocol at 0.15 Hz, while 7 of 14 completed 0.3 Hz and 13 of 14 completed 0.6 Hz. There were no changes in simple visual-spatial cognitive tests, e.g., mental rotation or match-to-sample. There were significant deficits during 0.15 Hz adaptation in both accuracy and reaction time during a spatial reference task in which subjects are asked to identify a match of a 3D reoriented cube assemblage. Our results are consistent with antidotal reports of cognitive impairment that are common during sensorimotor adaptation with G-transitions. We conclude that these cognitive deficits stem from the ambiguity of spatial reference frames for central processing of inertial motion cues.

Kayanickupuram, A. J.↗

DOE OSTI2022

Indirect Routes to Aminoacyl-tRNA: The Diversity of Prokaryotic Cysteine Encoding Systems

Universally present aminoacyl-tRNA synthetases (aaRSs) stringently recognize their cognate tRNAs and acylate them with one of the proteinogenic amino acids. However, some organisms possess aaRSs that deviate from the accurate translation of the genetic code and exhibit relaxed specificity toward their tRNA and/or amino acid substrates. Typically, these aaRSs are part of an indirect pathway in which multiple enzymes participate in the formation of the correct aminoacyl-tRNA product. The indirect cysteine (Cys)-tRNA pathway, originally thought to be restricted to methanogenic archaea, uses the unique O -phosphoseryl-tRNA synthetase (SepRS), which acylates the non-proteinogenic amino acid O -phosphoserine (Sep) onto tRNA Cys . Together with Sep-tRNA:Cys-tRNA synthase (SepCysS) and the adapter protein SepCysE, SepRS forms a transsulfursome complex responsible for shuttling Sep-tRNA Cys to SepCysS for conversion of the tRNA-bound Sep to Cys. Here, we report a comprehensive bioinformatic analysis of the diversity of indirect Cys encoding systems. These systems are present in more diverse groups of bacteria and archaea than previously known. Given the occurrence and distribution of some genes consistently flanking SepRS, it is likely that this gene was part of an ancient operon that suffered a gradual loss of its original components. Newly identified bacterial SepRS sequences strengthen the suggestion that this lineage of enzymes may not rely on the m 1 G37 identity determinant in tRNA. Some bacterial SepRSs possess an N-terminal fusion resembling a threonyl-tRNA synthetase editing domain, which interestingly is frequently observed in the vicinity of archaeal SepCysS genes. We also found several highly degenerate SepRS genes that likely have altered amino acid specificity. Cross-analysis of selenocysteine (Sec)-utilizing traits confirmed the co-occurrence of SepCysE and the Sec-utilizing machinery in archaea, but also identified an unusual O -phosphoseryl-tRNA Sec kinase fusion with an archaeal Sec elongation factor in some lineages, where it may serve in place of SepCysE to prevent crosstalk between the two minor aminoacylation systems. These results shed new light on the variations in SepRS and SepCysS enzymes that may reflect adaptation to lifestyle and habitat, and provide new information on the evolution of the genetic code.

59 BASIC BIOLOGICAL SCIENCES↗

NASA NTRS2012

Visual perception system and method for a humanoid robot

A robotic system includes a humanoid robot with robotic joints each moveable using an actuator(s), and a distributed controller for controlling the movement of each of the robotic joints. The controller includes a visual perception module (VPM) for visually identifying and tracking an object in the field of view of the robot under threshold lighting conditions. The VPM includes optical devices for collecting an image of the object, a positional extraction device, and a host machine having an algorithm for processing the image and positional information. The algorithm visually identifies and tracks the object, and automatically adapts an exposure time of the optical devices to prevent feature data loss of the image under the threshold lighting conditions. A method of identifying and tracking the object includes collecting the image, extracting positional information of the object, and automatically adapting the exposure time to thereby prevent feature data loss of the image.

Wells, James W.↗