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A site-directed mutagenesis analysis of tNOX functional domains

Constitutive NADH oxidase proteins of the mammalian cell surface exhibit two different activities, oxidation of hydroquinones (or NADH) and protein disulfide-thiol interchange which alternate to yield oscillatory patterns with period lengths of 24 min. A drug-responsive tNOX (tumor-associated NADH oxidase) has a period length of about 22 min. The tNOX cDNA has been cloned and expressed. These two proteins are representative of cycling oxidase proteins of the plant and animal cell surface. In this report, we describe a series of eight amino acid replacements in tNOX which, when expressed in Escherichia coli, were analyzed for enzymatic activity, drug response and period length. Replacement sites selected include six cysteines that lie within the processed plasma membrane (34 kDa) form of the protein, and amino acids located in putative drug and adenine nucleotide (NADH) binding domains. The latter, plus two of the cysteine replacements, resulted in a loss of enzymatic activity. The recombinant tNOX with the modified drug binding site retained activity but the activity was no longer drug-responsive. The four remaining cysteine replacements were of interest in that both activity and drug response were retained but the period length for both NADH oxidation and protein disulfide-thiol interchange was increased from 22 min to 36 or 42 min. The findings confirm the correctness of the drug and adenine nucleotide binding motifs within the tNOX protein and imply a potential critical role of cysteine residues in determining the period length.

Non-NASA Center↗

Overview and Initial Results of SAND-E: Semi-Autonomous Navigation for Detrital Environments

Unmanned aerial systems (UAS) and automated terrain analysis for science and navigation are new technologies for planetary exploration. The Mars Helicopter will fly with the Mars2020 rover, the Dragonfly quadcopter will explore Titan, and Soil Properties and Object Classification (SPOC) software will be used for path planning and navigation on the Mars2020 rover. Using an Argo J5 rover instrumented with stereo cameras and Autonomous Soil Assessment System (ASAS) software, and an off the shelf quadcopter, SAND-E tested the use of automated terrain analysis and UAS data for science operations in a Mars-analog environment in Iceland during July of 2019. Scientifically, we sought to determine changes in the physical and chemical properties of sediments along a glacial-fluvial-aeolian transport pathway. Operationally, we tested rover mission-like scenarios that included UAS images and classified terrain images. Here, we present the initial results for both the operations and science elements of the study. Site Selection: A goal of SAND-E is examine sorting and alteration of sediments in fluvial and aeolian environments in both mineral-dominated and glass-dominated basaltic settings. During the first year of the project we focused on a mineral-dominated environment. Selection of the location was based on prior publications that indicated our selected region had a greater abundance of crystalline sediments than other areas fluvial-aeolian settings in Iceland. Other criteria included the presence of both fluvial and aeolian landforms along a transport pathway such that the sediments in transport could be linked to their source rocks. We chose the Skjaldbreidauhraun glacial outwash plain, which sits at the base of Thórisjökull glacier. The site is 30 km north of Thingvellir National Park and ~2 hours from Reykjavik. The outwash plain is fed by two small catchments that drain from the base of the glacier and cut through hyaloclastite and shield volcano bedrock. The drainage progresses from steep alluvial fans near the glacier into a low-sloping fluvial braidplain that becomes confined by the Skjaldbreidur shield volcano and creates a shallow canyon cut into lava bedrock. The fluvial system was a typical braided alluvial environment composed pebble- and cobble-bedded longitudinal bars and sandy channel beds. The river remained active and fluctuated in response to diurnal runoff cycles near the glacier before disappearing into the sandy substrate downstream. The high concentration of suspended sediment in the river was evident by the cloudy water and the silt and clay-sized sediments that draped the channel beds after abandonment and created playas in the lowest sloping areas of the catchment. The entire fluvial system was affected by the winds generated by frontal systems and katabatic flows descending the glacier. This resulted in the formation of aeolian lag deposits and a wind-deflation plain where the fluvial system was not active. Wind ripples and drifts formed in abandoned fluvial channels from aeolian reworking of the sand-sized fluvial sediments. The silt- and clay-sized sediments found in fluvial channels, bar tops, and playas generated dust plumes during high wind events. Our operation sought to capture the variability in this system by sampling from the range of fluvial and aeolian features 6.3 km (proximal), 11.3 km (medial), and 14.4 km (distal) along the river from its origin at the base of glacier.

Ewing, R. C.↗

Scientific investigations at a lunar base

Studies of lunar origin and history, astronomy, and applications of lunar environmental conditions to experiments in physics and chemistry are examined to gain some insight into their control over site selection and base characteristics. Lunar studies most likely will be distinguished by needs for surface mobility and laboratory support; astronomy will require initial construction and servicing by crews on a sporadic basis; physics and chemistry experiments will require complex facilities and the ability to support a research staff and supporting laboratory and fabrication shop facilities. Lunar bases dominated by lunar investigations will be sited relatively near important lunar geological features. Astronomical facilities may require access to the lunar limb or poles. Physics and chemistry facilities are probably not strongly tied to the specific base locations.

Duke, M. B.↗

Experiment M0003-4: Advanced solar cell and coverglass analysis, an overview

The experiment consists of 51 coverglass samples and 12 solar cell strings. Sixteen (16) of the coverglass samples were on the leading edge and 16 on the trailing edge. An additional 16 samples were used as control samples and were not flown. The scenario for analysis was established for the coverglasses as follows. A photographic and a Nomarski survey were taken. Selected sites were then analyzed in their 'dirty' condition for optical transmission, reflectance, and absorbance. The coverglass samples have yet to be cleaned and resulting residue analyzed. The next stage of the analysis of the filters will be by probing through their layers to evaluate the multilayers used in their design. The solar cells were visually inspected and photographed. Tape recorder data is being analyzed and selected cell strings are being evaluated for their electrical properties. The first phase of the optical studies were completed.

Terry M Trumble↗

Towards a Mars base - Critical steps for life support on the moon and beyond

In providing crew life support for future exploration missions, overall exploration objectives will drive the life support solutions selected. Crew size, mission tasking, and exploration strategy will determine the performance required from life support systems. Human performance requirements, for example, may be offset by the availability of robotic assistance. Once established, exploration requirements for life support will be weighed against the financial and technical risks of developing new technologies and systems. Other considerations will include the demands that a particular life support strategy will make on planetary surface site selection, and the availability of precursor mission data to support EVA and in situ resource recovery planning. As space exploration progresses, the diversity of life support solutions that are implemented is bound to increase.

Rummel, John D.↗

The Second Conference on Lunar Bases and Space Activities of the 21st Century, volume 2

These 92 papers comprise a peer-reviewed selection of presentations by authors from NASA, the Lunar and Planetary Institute (LPI), industry, and academia at the Second Conference on Lunar Bases and Space Activities of the 21st Century. These papers go into more technical depth than did those published from the first NASA-sponsored symposium on the topic, held in 1984. Session topics included the following: (1) design and operation of transportation systems to, in orbit around, and on the Moon; (2) lunar base site selection; (3) design, architecture, construction, and operation of lunar bases and human habitats; (4) lunar-based scientific research and experimentation in astronomy, exobiology, and lunar geology; (5) recovery and use of lunar resources; (6) environmental and human factors of and life support technology for human presence on the Moon; and (7) program management of human exploration of the Moon and space.

Mendell, Wendell W.↗

Thermal power systems small power systems applications project. Volume 2: Detailed report

Small power system technology as applied to power plants up to 10 MW in size was considered. Markets for small power systems were characterized and cost goals were established for the project. Candidate power plant system design concepts were selected for evaluation and preliminary performance and cost assessments were made. Breakeven capital costs were determined for leading contenders among the candidate systems. The potential use of small power systems in providing part of the demand for pumping power by the extensive aqueduct system of California, was studied. Criteria and methodologies were developed for the ranking of candidate power plant system design concepts. Experimental power plant concepts of 1 MW rating were studied to define a power plant configuration for subsequent detail design construction, testing and evaluation. Site selection criteria and ground rules were developed.

Marriott, A. T.↗

Contractors Road Heavy Equipment Area SWMU 055 Corrective Measures Implementation Progress Report Kennedy Space Center, Florida

This Corrective Measures Implementation (CMI) Progress Report documents: (i) activities conducted as part of supplemental assessment activities completed from June 2009 through November 2014; (ii) Engineering Evaluation (EE) Advanced Data Packages (ADPs); and (iii) recommendations for future activities related to corrective measures at the Site. Applicable meeting minutes are provided as Appendix A. The following EE ADPs for CRHE are included with this CMI Progress Report: center dot Supplemental Site Characterization ADP (Step 1 EE) (Appendix B) center dot Site Characterization ADP (Step 1 EE) for Hot Spot 1 (HS1) (Appendix C) center dot Remedial Alternatives Evaluation (Step 2 EE) ADP for HS1 (Appendix D) center dot Interim Measures Work Plan (Step 3 EE) ADP for HS1 (Appendix E) center dot Site Characterization ADP (Step 1 EE) ADP for Hot Spot 2 (HS2), High Concentration Plume (HCP), and Low Concentration Plume (LCP) (Appendix F) A summary of direct-push technology (DPT) and groundwater monitoring well sampling results are provided in Appendices G and H, respectively. The Interim Land Use Control Implementation Plan (LUCIP) is provided as Appendix I. Monitoring well completion reports, other applicable field forms, survey data, and analytical laboratory reports are provided as Appendices J through M, respectively, in the electronic copy of this document. Selected Site photographs are provided in Appendix N. The interim groundwater monitoring plan and document revision log are included as Appendices O and P, respectively. KSC Electronic Data Deliverable (KEDD) files are provided on the attached compact disk.

Johnson, Jill W.↗

The Second Conference on Lunar Bases and Space Activities of the 21st Century, volume 1

These papers comprise a peer-review selection of presentations by authors from NASA, LPI industry, and academia at the Second Conference (April 1988) on Lunar Bases and Space Activities of the 21st Century, sponsored by the NASA Office of Exploration and the Lunar Planetary Institute. These papers go into more technical depth than did those published from the first NASA-sponsored symposium on the topic, held in 1984. Session topics covered by this volume include (1) design and operation of transportation systems to, in orbit around, and on the Moon, (2) lunar base site selection, (3) design, architecture, construction, and operation of lunar bases and human habitats, and (4) lunar-based scientific research and experimentation in astronomy, exobiology, and lunar geology.

Mendell, Wendell W.↗

Trajectory optimization for a National Launch System vehicle

A trajectory design process is presented for a proposed National Launch System (NLS) vehicle configuration. A software trajectory planner was developed to generate near optimal pitch plane trajectories for variable mission plans, variable launch site selections, and last minute wind conditions at the time of launch. In addition, the trajectory planner performs in-flight near optimal trajectory redesign in the event of an engine-out or unexpected wind dispersions. The trajectory planner iteratively simulates trial trajectories defined by variables corresponding to a parameterized trajectory shape. The trajectory planner optimizes these trajectory parameters according to the fuel used to realize the trajectories. For prelaunch scenarios, the trajectory planner generated near optimal trajectories of consistent fuel performance irrespective of the winds encountered at the launch site. In-flight trajectory redesigns were also shown to be effective in fulfilling the mission plan and preserving the structural integrity of the vehicle. The in-flight redesign results indicate that closed-loop guidance in the early stages of ascent is highly effective in accommodating engine failures or wind dispersions. All trajectory designs were verified using a 6DOF simulation with flight controllers.

Chen, Eugene S.↗

Effective Utilization of Commercial Wireless Networking Technology in Planetary Environments

The purpose of this research is to investigate the use of commercial, off-the-shelf wireless networking technology in planetary exploration applications involving rovers and sensor webs. The three objectives of this research project are to: 1) simulate the radio frequency environment of proposed landing sites on Mars using actual topographic data, 2) analyze the performance of current wireless networking standards in the simulated radio frequency environment, and 3) propose modifications to the standards for more efficient utilization. In this annual report, we present our results for the second year of research. During this year, the effort has focussed on the second objective of analyzing the performance of the IEEE 802.11a and IEEE 802.1lb wireless networking standards in the simulated radio frequency environment of Mars. The approach builds upon our previous results which deterministically modelled the RF environment at selected sites on Mars using high-resolution topographical data. These results provide critical information regarding antenna coverage patterns, maximum link distances, effects of surface clutter, and multipath effects. Using these previous results, the physical layer of these wireless networking standards has now been simulated and analyzed in the Martian environment. We are looking to extending these results to the and medium access layer next. Our results give us critical information regarding the performance (data rates, packet error rates, link distances, etc.) of IEEE 802.1 la/b wireless networks. This information enables a critical examination of how these wireless networks may be utilized in future Mars missions and how they may be possibly modified for more optimal usage.

Caulev, Michael↗

Large Grooves in the South Polar Layered Deposits: Insights from Spacecraft Data and Terrestrial Analogs

The Martian polar layered deposits (PLD) are probably the best source of information about the recent climate history of Mars, but their origin and the mechanisms of accumulation are still a mystery. The polar layers are sedimentary deposits that most planetary scientists believe are composed of water ice and varying amounts of wind-blown dust, although their composition is poorly constrained. Interpretation of the observed polar stratigraphy in terms of global climate changes is complicated by the significant difference in surface ages between the north and south PLD inferred from crater statistics. The study reported here was undertaken as part of the landing site selection effort for the Mars Polar Lander (MPL) and Deep Space 2 (DS2) missions that made use of all available data. We used Mariner 9, Viking, and Mars Global Surveyor images of the south PLD in the area accessible to MPL and DS2 to evaluate the topography and morphology of grooves, terraced layers, and other features. Here we report on results from grooves that appear to have been carved by strong winds. Because these features are found throughout the interior of the PLD, where MPL and DS2 were targeted to land, their topographic characteristics were judged as important input for landing site safety assessment. The characteristics of the grooves also provide constraints and insights into aeolian processes in the polar regions and the effects these have on PLD ablation. Our results indicate that these grooves do not represent landing hazards at the scale of the images (approx. 80 m/pixel). Their topography and shape do not seem correlated with south PLD layering. No Earth analog of suitable scale exists, although the grooves bear some resemblance to smaller terrestrial deflation hollows found in soft sediment and ice. Additional information is contained in original extended abstract.

Bridges, N. T.↗

HEDS Goals

In November of 1996, NASA made the decision to fully integrate several areas of robotic and human Mars exploration study and planning. As a result of this decision, requirements for unmanned robotic missions to support human Mars exploration were identified and a plan to meet these requirements was developed. Concrete progress in the implementation of this plan has been made. Three experiments have been selected and are in development for the Mars Surveyor Program 2001 Orbiter and Lander missions which will provide critical data for the planning of human missions to Mars. An Announcement of Opportunity for the Mars Surveyor Program 2003 Lander mission has recently been released which solicited proposals related to planning for a human mission. In order to define HEDS objectives for Mars robotic missions, it is important to understand what information is required as a foundation for mounting a program of exploration of this magnitude. We identify areas of research on robotic missions that will enable future human missions. These areas include Site Selection for Human Missions, Hazards to Human Explorers, Living off the Land, and Testing Critical Technologies in the Mars Environment.

Pline, A.↗

Application of Spaceborne Scatterometer for Mapping Freeze-Thaw State in Northern Landscapes as a Measure of Ecological and Hydrological Processes

Landscape freeze/thaw transitions coincide with marked shifts in albedo, surface energy and mass exchange, and associated snow dynamics. Monitoring landscape freeze/thaw dynamics would improve our ability to quantify the interannual variability of boreal hydrology and river runoff/flood dynamics. The annual duration of frost-free period also bounds the period of photosynthetic activity in boreal and arctic regions thus affecting the annual carbon budget and the interannual variability of regional carbon fluxes. In this study, we use the NASA scatterometer (NSCAT) to monitor the temporal change in the radar backscatter signature across selected ecoregions of the boreal zone. We have measured vegetation tissue temperatures, soil temperature profiles, and micrometeorological parameters in situ at selected sites along a north-south transect extending across Alaska from Prudhoe Bay to the Kenai Peninsula and in Siberia near the Yenisey River. Data from these stations have been used to quantify the scatterometer's sensitivity to freeze/thaw state under a variety of terrain and landcover conditions. Analysis of the NSCAT temporal response over the 1997 spring thaw cycle shows a 3 to 5 dB change in measured backscatter that is well correlated with the landscape springtime thaw process. Having verified the instrument's capability to monitor freeze/thaw transitions, regional scale mosaicked data are applied to derive temporal series of freeze/thaw transition maps for selected circumpolar high latitude regions. These maps are applied to derive areal extent of frozen and thawed landscape and demonstrate the utility of spaceborne radar for operational monitoring of seasonal freeze-thaw dynamics and associated biophysical processes for the circumpolar high latitudes.

McDonald, Kyle↗

Evaluation of the NASA Artemis Regions of Interest for ISRU Water Mine Potential

The NASA Artemis Campaign has a stated goal to return to the Moon to maintain a sustainable presence; In-Situ Resource Utilization (ISRU) is a key part of sustainability. The regions of interest identified for the Artemis campaign are at Lunar the South Pole where water ice, a valuable resource for ISRU, has been identified. As such, a preliminary evaluation of the ISRU ice mining potential has been performed for of these regions of interest. A set of ground rules for this evaluation were developed to align with current assumptions for customer needs, hardware capabilities, an initially limited infrastructure, and lunar environments/terrain. These ground rules, and the evaluation of six regions of interest, are presented here. The site selections (ISRU and customer assets) and their associated traverses are notional and were intended only to provide a broad preliminary evaluation of the water ISRU potential of the regions. Evaluation of these regions are subject to change as decisions regarding utilization are made. Water ISRU is possible at all regions, though the degree to which each criterion are met is variable. The two regions near Shackleton ranked highest in this evaluation, while the de Gerlache region presented the most difficulties meeting the current criteria. The regions were not explicitly ranked due to the nuances associated with the high number of variables but evaluation summaries of each are presented. It should also be noted that all ISRU ‘mine’ sites in this analysis focused on smaller (few kilometer) size permanently shadowed regions (PSRs). This was necessary to meet proximity requirements between these PSRs and the highly illuminated regions needed for customers and ISRU processing. The areas identified in this study are meant to focus exploration and reconnaissance efforts needed to better evaluate the ISRU potential.

In-situ resource utilization↗

Evaluation of the NASA Artemis Regions of Interest for ISRU Water Mine Potential

The NASA Artemis Campaign has a stated goal to return to the Moon to maintain a sustainable presence; In-Situ Resource Utilization (ISRU) is a key part of sustainability. The regions of interest identified for the Artemis campaign are at Lunar the South Pole where water ice, a valuable resource for ISRU, has been identified. As such, a preliminary evaluation of the ISRU ice mining potential has been performed for of these regions of interest. A set of ground rules for this evaluation were developed to align with current assumptions for customer needs, hardware capabilities, an initially limited infrastructure, and lunar environments/terrain. These ground rules, and the evaluation of six regions of interest, are presented here. The site selections (ISRU and customer assets) and their associated traverses are notional and were intended only to provide a broad preliminary evaluation of the water ISRU potential of the regions. Evaluation of these regions are subject to change as decisions regarding utilization are made. Water ISRU is possible at all regions, though the degree to which each criterion are met is variable. The two regions near Shackleton ranked highest in this evaluation, while the de Gerlache region presented the most difficulties meeting the current criteria. The regions were not explicitly ranked due to the nuances associated with the high number of variables but evaluation summaries of each are presented. It should also be noted that all ISRU ‘mine’ sites in this analysis focused on smaller (few kilometer) size permanently shadowed regions (PSRs). This was necessary to meet proximity requirements between these PSRs and the highly illuminated regions needed for customers and ISRU processing. The areas identified in this study are meant to focus exploration and reconnaissance efforts needed to better evaluate the ISRU potential.

In-situ resource utilization↗

Global 3D Data Visualization and Analysis Platform With Advanced Machine Learning Capabilities in Support of Lunar Exploration

Introduction: The science goals for NASA’s Artemis program include: a) Understanding the character and origin of lunar polar volatiles, b) Conducting experimental science in the lunar environment and c) Investigating and mitigating exploration risks [1]. The permanently shadowed regions (PSRs) on the Lunar south pole are expected to host large quantities of water-ice and volatiles that are important for sustainable Lunar exploration [2]. There are several missions such as onboard Korea Pathfinder Lunar Orbiter (KPLO: Korean name Danuri) with onboard ShadowCam camera [3], Astrobotic Peregrine Mission One [4], and other efforts underway to obtain high resolution topographic, minerals, volatiles and other information on the moon. We envision a need in immediate future for platforms to integrate these data sets, provide rendering and visualization capabilities in the context of a 3D Lunar globe for easier information access and analysis. NASA's Celestial Mapping System (CMS) [5] is developed to address the need for 3D tools for planetary science investigations, mission planning, in-situ operations, in a 3D-first design constructed around a unified view of a planetary globe. At present CMS provides many critical functionalities that include: 1) equipment planning and optimized placement on Lunar surface 2) line-of-sight (LOS) analysis 3) powerful measurement tools based on 3D terrain with realistic 3D models to represent rovers, astronauts and equipment 4) visualization of derived mapping products (e.g. resource maps), and 5) a data engine for hosting new observations that are not available in other contemporary lunar data tools [5, 7]. Planetary Data Ingestion: CMS can consume and analyze data from locally hosted and external third party sources. It is compatible with Open Geospatial Consortium (OGC) data and file standards and currently integrates datasets from the Astrogeology Science Center of USGS. This includes global and local data acquired from NASA (LRO, Clementine, Lunar Orbiter) and JAXA (SELENE/Kaguya), with capability of integrating more datasets. In addition, users can specify other WMS-hosted data endpoints, which CMS can then query and stream data from automatically. To set-up an automated process for ingestion and accurate rendering, visualization and analysis of external 3rd party planetary datasets within CMS, we initiated the process of ingesting unique dataset of super-enhanced images of the permanently shadowed regions (PSRs) at the lunar poles which were produced by the Hyper-effective nOise Removal U-net Software (HORUS) tool [8]. This tool was developed to enhance the extremely low-light images of the interior of PSRs and provide the ability to see within these regions at and discern surface features (i.e. boulders and craters) down to 3 meters in size. We focused on the Nobile region on the Lunar south pole, selected site for VIPER mission and stitched several images to create a high-resolution map within one of the PSR of Nobile crater. Figure 1 shows the dark PSR zone form the original NAC layer of LRO as the base layer (left image) and the illuminated areas within that crater (center) which was created by ingesting and merging several of HORUS generated images. At present we employ a semi-automated process to ensure spatial accuracy and merger of several overlapping zones. However, we are in the process of completely automating this process by employing AI based techniques that would rank, sort, and stack the images based on their information density. The georectification of the images would employ selected features. Analysis on Ingested Planetary Datasets: Once an external planetary data-set is successfully ingested, georectified and merged seamlessly as a data-layer; CMS’ numerous analysis tools can be used on this data. A Line of Sight (LOS) tool has been developed for CMS which analyzes terrain profiles and obstructions to determine visibility for remote observers [5,6]. Figure 1 (right image) shows the viewshed analysis on the same PSR in the Nobile region. The yellow pin shows the observer location outside the PSR. The yellow area shows the visible part of PSR. The obstructed area with no visibility for the observer is shown in red. The Measurements tool allows the user to take area and distance measurements of features on the terrain using various shapes. Measurement type can be specified in a number of ways: Line, Path, Polygon, Circle, Ellipse, Square, Rectangle or Freehand. Once the shape is specified, elevation information can then be extracted along each of these shapes. Figure 2 (left) shows the measurements performed on a crater n illuminated PSR in Nobile region. The equipment placement tool allows the user to place a 3D equipment model at a desired location and analyze its coverage area. The equipment placement tool is coupled with LOS to determine the coverage. Figure 2 (right) shows an equipment placed on the Lunar terrain and it’s coverage area. The red rays are blocked sight lines and the green rays are non-obstructed sight lines with the cyan lines showing the point of intersection with the terrain. More details are provided in the video demonstrations in Reference 5. Overcoming Polar Distortions: 3D geospatial applications exhibit significant distortions in polar imagery due to several reasons: 1) distortions in the source imagery, 2) incompatible tessellation algorithms at the poles, and 3) map projections. We are leveraging new tessellation algorithms and reprojecting data using projections that are better suited for Lunar poles. The goal is to seamlessly switch to polar projections while maintaining 3D view and navigation.

Maps↗