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At least 235 records · Page 13

The response of single human cells to zero gravity

The SO15 experiment was designed to extend observations of the effects of zero-gravity to living human cells during and subsequent to a 59-day flight on Skylab 3. A strain of diploid human embryonic lung cells, WI-38, was chosen for this purpose. The studies were concerned with observations designed to detect the effects of zero-gravity on cell growth rates and on cell structure as observed by light microscopy, transmission and scanning electron microscopy and histochemistry. Studies of the effects of zero-gravity on the cell function and the cell cycle were performed by time lapse motion picture photography and microspectrophotometry. Subsequent study of the returned living cells included karotyping, G- and C-banding, and analyses of the culture media used. Some of the living cells returned were banked by deep freeze techniques for possible future experiments.

Montgomery, P. O., Jr.↗

Multi-slit spectrograph and H alpha Doppler system

Description of the design and operation of a multiple-entrance-slit spectrograph, built for time-lapse photography of the spectra of flares and other transient solar phenomena. This spectrograph employs narrow-band filters to limit the wavelength range of the spectrograph to a few angstroms centered at H-alpha or other spectral lines. A polarizing beamsplitter, prior to the slits, provides a prefiltered second solar image to a narrow band H-alpha Doppler filter for simultaneous photography in the wings of the H-alpha line. The spectrograph and the H-alpha Doppler system constitute a flexible instrument in which components may be substituted to achieve different passband widths, image scales, dispersions, and corresponding numbers and spacings of adjacent spectra at a selected wavelength.

Martin, S. F.↗

Digital registration of ERTS-1 imagery

Two requirements for the registration of Earth Resources Technology Satellite (ERTS) data are discussed. These requirements are registration of ERTS data acquired on separate passes and registration of ERTS data to a ground reference. Performances of a semi-automatic warp algorithm and an automatic pipeline processing algorithm demonstrate that either procedure is useful, depending upon the user's requirements. In two cases where the time lapse between passes of the satellite were 90 days and 18 days the automatic pipeline processor reduced the mean radial registration error to 0.28 and 0.58 pixel, respectively. It is concluded that this technique is promissing for high-volume production processing.

Bonrud, L. O.↗

The response of single human cells to zero gravity

Twenty separate cultures of Wistar-38 human embryonic lung cells were exposed to a zero-gravity environment on Skylab for periods of time ranging from one to 59 days. Duplicate cultures were run concurrently as ground controls. Ten cultures were fixed on board the satellite during the first 12 days of flight. Growth curves, DNA microspectrophotometry, phase microscopy, and ultrastructural studies of the fixed cells revealed no effects of a zero-gravity environment on the ten cultures. Two cultures were photographed with phase time lapse cinematography during the first 27 days of flight. No differences were found in mitotic index, cell cycle, and migration between the flight and control cells. Eight cultures were returned to earth in an incubated state. Karyotyping and chromosome banding tests show no differences between the flight and control cells.

Montgomery, P. O., Jr.↗

Image animation for theme enhancement and change detection

Animated displays are useful in enhancing subtle temporally related changes in scenes viewed by satellites capable of providing repetitive coverage. The detectability of fixed features is also improved through the help of the powerful visual integration process. To expedite the process of assembling and displaying well-registered, time-lapse sequences and to provide means for making quantitative measurements of radiances, displacements, and areas, an electronic satellite image analysis console was constructed. During the LANDSAT-1 program, this equipment was applied to the needs of a number of earth resource investigators with interests principally related to dynamic hydrology. The measurement of the areal extent of snow cover within defined drainage basins is discussed as a representative applications example.

Evans, W. E.↗

Flight 1 technical report for experiment 74-37 contained polycrystalline solidification in low-G

A .005 M solution of fluorescein in cyclohexanol was directionally solidified in a standard 10 x 10 x 45mm UV silica cuvette, using a bottom thermoelectric chilling device. Progress of the experiment was monitored by time lapse photography. During flight (SPAR I) the camera malfunctioned and only one quarter of the expected data were collected. Comparison of flight and ground specimens indicated that: (1) The dark green layer observed ahead of the solid-liquid interface which is most likely the solute-enriched zone, appears to be wider in the flight specimen; (2) Parasitic nucleation ahead of the solid-liquid interface in the flight sample led to an irregularly shaped interface, smaller grain size, equiaxed grain morphology and a larger average macroscopic growth rate; (3) The formation of equiaxed grains ahead of the solid-liquid interface in the flight specimen may be attributed to ordered islands within the liquid, which survived remelting because of the low degree of superheating (approximately equal to 1.5 C), did not settle because of reduced gravity and acted as nuclei during cooling.

Papaziak, J. M.↗

Application of LANDSAT MSS data to the study of oceanographical environment

The author has identified the following significant results. LANDSAT MSS data of a three year time lapse indicate change of sea surface condition in Seto Inland Sea and coastal region. The red tide which formerly concentrated in the bay or inland sea now extends into an open sea. A small ocean vortex similar to mesoscale atmospheric vortex is revealed by the band 4 image of the satellite data. A manual photographic method applied to a single band image of MSS is effective in detecting sea surface pollution.

Maruyasu, T.↗

Environmental change pattern in central Japan as revealed by LANDSAT data

The author has identified the following significant results. A patched cirrus reported by weather stations was hardly recognizable by the eye in LANDSAT MSS data of a three year time lapse. The cloud cover affected radiance values significantly in band 4, while its effect was minimal in bands 6 and 7 (near infrared spectra). The cross correlation coefficient analysis between the two images indicated that the highest value obtained in central Japan was 0.963 for the area where little change occurred in land use over the three period. An analysis of land use in Nagoya showed little change in the metropolitan area while a fairly large change occurred in the northern periphery of the city where large scale housing projects are located.

Maruyasu, T.↗

H-alpha macrospicules - Identification with EUV macrospicules and with flares in X-ray bright points

The paper presents observational evidence that two newly observed transient solar phenomena, EUV macrospicules and X-ray bright-point flares, are closely related. Time-lapse H-alpha filtergram observations of the limb in quiet regions show small surgelike eruptions called H-alpha macrospicules. From the similarity of H-alpha macrospicules and EUV macrospicules, and from comparison of simultaneous H-alpha and He II 304 A observations, we conclude that H-alpha macrospicules are EUV macrospicules viewed in H-alpha, although most EUV macrospicules are too faint in H-alpha to appear on H-alpha filtergrams of normal exposure. From comparison of simultaneous X-ray and H-alpha observations of flares in X-ray bright points situated on the limb, we show that flares in X-ray bright points often produce H-alpha macrospicules.

Moore, R. L.↗

Cinemicrographic specimen housing

Housing used to observe gravitation effects on specimens embedded in support media, such as agar, supports microbial specimens vertically for time-lapsed cinemicrographic studies. Procedure cannot be performed with conventional microscopes which see specimens in horizontal plane only.

Wilkins, J. R.↗

Impact of Short Interval SMS Digital Data on Wind Vector Determination for a Severe Local Storms Area

The impact of 5 minute interval SMS-2 visible digital image data in analyzing severe local storms is examined using wind vectors derived from cloud tracking on time lapsed sequence of geosynchronous satellite images. The cloud tracking areas are located in the Central Plains, where on 6 May 1975, hail-producing thunderstorms occurred ahead of a well defined dry line. The results demonstrate that satellite-derived wind vectors and their associated divergence fields complement conventional meteorological analyses in describing the conditions preceding severe local storm development.

Peslen, C. A.↗

Ice crystal growth in a dynamic thermal diffusion chamber

Ice crystals were grown in a supersaturated environment produced by a dynamic thermal diffusion chamber, which employed two horizontal plates separated by a distance of 2.5 cm. Air was circulated between and along the 1.2 m length of the plates past ice crystals which nucleated and grew from a fiber suspended vertically between the two plates. A zoom stereo microscope with a magnification which ranged from 3X to 80X and both 35 mm still photographs and 16 mm time lapse cine films taken through the microscope were used to study the variation of the shape and linear growth rate of ice crystals as a function of the ambient temperature, the ambient supersaturation, and the forced ventilation velocity. The ambient growth conditions were varied over the range of temperature 0 to -40 C, over the range of supersaturation 4% to 50% with respect to ice, and over the range of forced ventilation velocities 0 cm/s to 20 cm/s.

Keller, V. W.↗

Residual nutational activity of the sunflower hypocotyl in simulated weightlessness

The gravity dependence of circumnutational activity in the sunflower hypocotyl is investigated under conditions of simulated weightlessness. Seedling cultures of the sunflower Helianthus annuus were placed four days after planting in clinostats rotating at a rate of 1.0 rpm in the horizontal or somersaulting configurations, and plant movements around their growth axes were recorded in infrared light by a time-lapse closed-circuit video system. The amplitudes and mean cycle durations of the plant nutations in the horizontal and tumbling clinostats are observed to be 20% and 72%, and 32% and 74%, respectively, of the values observed in stationary plants; extrapolations to a state of zero g by the imposition of small centripetal forces on horizontally clinostated plants also indicate some nutational motion in the absence of gravity. It is concluded that the results are incompatible with the model of Israelsson and Johnsson (1967) of geotropic response with overshoot for sunflower circumnutation; however, results of the Spacelab 1 mission experiment are needed to unambiguously define the role of gravitation.

Chapman, D. K.↗

A regression technique for evaluation and quantification for water quality parameters from remote sensing data

The paper attempts to define optical physics and/or environmental conditions under which the linear multiple-regression should be applicable. It is reported that investigation of the signal response shows that the exact solution for a number of optical physics conditions is of the same form as a linearized multiple-regression equation, even if nonlinear contributions from surface reflections, atmospheric constituents, or other water pollutants are included. Limitations on achieving this type of solution are defined. Laboratory data are used to demonstrate that the technique is applicable to water mixtures which contain constituents with both linear and nonlinear radiance gradients. Finally, it is concluded that instrument noise, ground-truth placement, and time lapse between remote sensor overpass and water sample operations are serious barriers to successful use of the technique.

Whitlock, C. H.↗

Temporal variations of loop structures in the solar atmosphere

The temporal characteristics of coronal loops are described using a number of unique time-lapse sequences of Ne VII 465 A, Mg IX 368 A, and Fe XV 284 A spectroheliograms. These sequences were obtained with the NRL Skylab/ATM slitless spectrograph during the final Skylab mission (November 1973-February 1974). In the first sequence, Ne VII and Mg IX images were obtained simultaneously at 1.5-mm intervals for a 27-min duration on Nov. 28, 1973. In the second sequence, simultaneous Ne VII-Mg IX pairs were obtained at approximately 3-hr intervals (on alternate satellite orbits) for a 36-hr duration on Jan. 19-20, 1974. The intervening orbits were used to obtain a third sequence which consisted of Fe XV images at 3-hr intervals for the same 36-hr duration. The results place severe constraints on theoretical models of coronal heating and mass flow.

Sheeley, N. R., Jr.↗

Voyager image processing at the Image Processing Laboratory

This paper discusses new digital processing techniques as applied to the Voyager Imaging Subsystem and devised to explore atmospheric dynamics, spectral variations, and the morphology of Jupiter, Saturn and their satellites. Radiometric and geometric decalibration processes, the modulation transfer function, and processes to determine and remove photometric properties of the atmosphere and surface of Jupiter and its satellites are examined. It is exhibited that selected images can be processed into 'approach at constant longitude' time lapse movies which are useful in observing atmospheric changes of Jupiter. Photographs are included to illustrate various image processing techniques.

Jepsen, P. L.↗

Short-interval SMS wind vector determinations for a severe local storms area

Short-interval SMS-2 visible digital image data are used to derive wind vectors from cloud tracking on time-lapsed sequences of geosynchronous satellite images. The cloud tracking areas are located in the Central Plains, where on May 6, 1975 hail-producing thunderstorms occurred ahead of a well defined dry line. Cloud tracking is performed on the Goddard Space Flight Center Atmospheric and Oceanographic Information Processing System. Lower tropospheric cumulus tracers are selected with the assistance of a cloud-top height algorithm. Divergence is derived from the cloud motions using a modified Cressman (1959) objective analysis technique which is designed to organize irregularly spaced wind vectors into uniformly gridded wind fields. The results demonstrate the feasibility of using satellite-derived wind vectors and their associated divergence fields in describing the conditions preceding severe local storm development. For this case, an area of convergence appeared ahead of the dry line and coincided with the developing area of severe weather. The magnitude of the maximum convergence varied between -10 to the -5th and -10 to the -14th per sec. The number of satellite-derived wind vectors which were required to describe conditions of the low-level atmosphere was adequate before numerous cumulonimbus cells formed. This technique is limited in areas of advanced convection.

Peslen, C. A.↗

Circumnutation augmented in clinostatted plants by a tactile stimulus

Dark-grown, 4-day old, Helianthus annuus seedlings were rotated for 20 hr on horizontal clinostats to minimize the amplitude of circumnutation. Then a Plexiglas sheet was placed gently against the tip of the cotyledons. By time-lapse video imaging (using intermittent IR illumination to which the plants were insensitive) movements of the clinostatted plants were observed before, during, and after the period of mechanical contact. Immediately after the Plexiglas sheet was removed residual nutation increased in amplitude almost three-fold, then declined over the next 7 hr to the prestimulation level. This demonstration of enhancement of circumnutation by mechanical contact is consistent with the model of an endogeneous oscillator that can be stimulated by factors other than gravity.

Chapman, D. K.↗