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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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At least 253 records · Page 14

Latent nitrate reductase activity is associated with the plasma membrane of corn roots

Latent nitrate reductase activity (NRA) was detected in corn (Zea mays L., Golden Jubilee) root microsome fractions. Microsome-associated NRA was stimulated up to 20-fold by Triton X-100 (octylphenoxy polyethoxyethanol) whereas soluble NRA was only increased up to 1.2-fold. Microsome-associated NRA represented up to 19% of the total root NRA. Analysis of microsomal fractions by aqueous two-phase partitioning showed that the membrane-associated NRA was localized in the second upper phase (U2). Analysis with marker enzymes indicated that the U2 fraction was plasma membrane (PM). The PM-associated NRA was not removed by washing vesicles with up to 1.0 M NACl but was solubilized from the PM with 0.05% Triton X-100. In contrast, vanadate-sensitive ATPase activity was not solubilized from the PM by treatment with 0.1% Triton X-100. The results show that a protein capable of reducing nitrate is embedded in the hydrophobic region of the PM of corn roots.

NASA Discipline Number 61-10↗

Enzyme activity in terrestrial soil in relation to exploration of the Martian surface

Sensitive tests for the detection of extracellular enzyme activity in Martian soil was investigated using simulated Martian soil. Enzyme action at solid-liquid water interfaces and at low humidity were studied, and a kinetic scheme was devised and tested based on the growth of microorganisms and the oxidation of ammonium nitrite.

Mclaren, A. D.↗

A study of the diurnal variation in the thermosphere as derived by satellite drag.

Results of an analysis performed on 29,574 densities derived from the drag of ten satellites to determine the amplitude and shape of the diurnal-variation curve for the thermosphere. Although a considerable amount of smoothing is inherent in the drag method, it seemed useful to determine whether any change in the shape of the diurnal-variation curve with height, latitude, and solar activity could be detected. None was detected: the curve remains remarkably stable, symmetric, with a maximum at 1420 hr LST and a minimum at 0220 hr LST. A systematic variation of the temperature range with height is observed when static models are used to derive it.

Jacchia, L. G.↗

In situ detection of microbial respiration in soils and salt flats

Increase in CO2 partial pressures over a desert soil treated with casamino-acids glucose solution correlated with bacterial growth. Few or no increases in numbers of bacteria or CO2 concentrations were noted in similar plots treated with water only or receiving no treatment. Growth in the soil appeared to be severely nutrient limited during the 10 day experiment. Especially rapid growth took place between the third and fifth day, when temperatures ranged from 0 deg. (night) to a maximum of 17.4 deg. (day). Under the conditions of the experiment, intermittent CO2 assay was an insensitive indicator of growth, possibly because of restiction of gas escape by the desert pavement or solution, exchange, or precipitation of carbonate, but more likely because of inefficient sealing of hoods to and below the soil surface. CO2 assay was unable to detect microbial successions. The unpredictable course of these successions, plus unpredictable relative retentions mitigates against assay of organic gases as reliable in situ detection of microbial activity, except perhaps in very alkaline environments such as Owens Lake salts.

Tew, R. W.↗

Very high energy gamma ray astronomy

Recent results in ground based very high energy gamma ray astronomy are reviewed. The various modes of the atmospheric Cerenkov technique are described, and the importance of cosmic ray rejection methods is stressed. The positive detections of the Crab pulsar that suggest a very flat spectrum and time-variable pulse phase are discussed. Observations of other pulsars (particularly Vela) suggest these features may be general. Evidence that a 4.8 hr modulated effect was detected from Cyg X-3 is strengthened in that the exact period originally proposed agrees well with a recent determination of the X-ray period. The southern sky observations are reviewed, and the significance of the detection of an active galaxy (NGC 5128) is considered for source models and future observations.

Grindlay, J. E.↗

Very high-energy gamma ray astronomy

Recent results in ground-based very high-energy (less than 10 to the eleventh power eV) gamma-ray astronomy are reviewed. The various modes of the atmospheric Cerenkov technique are described, and the importance of cosmic ray rejection methods is stressed. The positive detections (at approximately less than 10 to the 12th power eV) of the Crab pulsar that suggest a very flat spectrum and time-variable pulse phase are discussed. Observations of other pulsars (particularly Vela) suggest that these features may be general. The steady flux upper limits for the Crab Nebula are thus reconsidered, and a new value of the implied (Compton-synchrotron) magnetic field in the Nebula is reported. Evidence that a 4.8-hour modulated effect was detected at E sub gamma is less than 10 to the 12th power eV from Cyg X-3 is strengthened in that the exact period originally proposed agrees well with a recent determination of the X-ray period. The southern sky observations are reviewed, and the significance of the detection of an active galaxy (NGC 5128) is considered for source models and future observations.

Grindlay, J. E.↗

The surface of Mars - The view from the Viking 2 lander

Viking 2 lander began imaging the surface of Mars at Utopia Planitia on September 3, 1976. The surface is a boulder-strewn reddish desert cut by troughs that probably form a polygonal network. A plateau can be seen to the east of the spacecraft, which for the most probable lander location is approximately the dirction of a tongue of ejecta from the crater Mie. Boulders at the lander 2 site are generally more vesicular than those near lander 1. Fines at both lander sites appear to be very fine-grained and to be bound in a duricrust. The pinkish color of the sky, similar to that observed at the lander 1 site, indicates suspension of surface material. However, the atmospheric optical depth is less than that at the lander 1 site. After dissipation of a cloud of dust stirred during landing, no changes other than those stemming from sampling activities have been detected in the landscape. No signs of large organisms are apparent at either landing site.

Mutch, T. A.↗

Viking on Mars - The carbon assimilation experiments

A fixation of atmospheric carbon, presumably into organic form, occurs in Martian surface material under conditions approximating the actual Martian ones. The reaction showed the following characteristics. The amount of carbon fixed is small by terrestrial standards; highest yields were observed in the light, but some dark activity was also detected; and heating the surface material to 90 C for nearly 2 hours had no effect on the reaction, but heating to 175 C for 3 hours reduced it by nearly 90%. New data from Mars do not support an earlier suggestion that the reaction is inhibited by traces of water. There is evidence of considerable heterogeneity among different samples, but different aliquots from the same sample are remarkably uniform in their carbon-fixing capacity. In view of its thermostability it is unlikely that the reaction is biological.

Horowitz, N. H.↗

The environmental program at Kennedy Space Center - Baseline to monitoring

KSC has developed an environmental program to ensure that its activities do not adversely affect the surrounding environment. Two essential elements of the total program are the baseline and monitoring programs. The goal of the baseline program is to collect sufficient information about the environment prior to Shuttle launches so that adverse changes in the environment - if and when they occur after the Shuttle program becomes active - can be detected and cause-effect relationships established when possible. The goal of the monitoring program is to use information from the baseline program along with survey and sampling operations during the period of initial Shuttle launches to document adverse changes in the environment.

Knott, W. M.↗

Advanced sensors for spaceborne measurements of the earth's atmosphere

Concepts involved in remote sensing from space are reviewed, together with performance requirements of proposed and planned equipment. Attention is focused on measurements of the troposphere, particularly meteorological variables and chemical species. The principles of absorption, using either passive or active sensors, emission detection, fluorescence measurements, and EM radiation scattering are outlined. The proposed NASA Windsat, using a CO2 laser from the Orbiter or from an 800 km orbit spacecraft to measure low-level wind speeds is described, as are the uses of the AMTS atmospheric sounder instrument for IR atmospheric temperature sensing and a 20-channel microwave radiometer to detect moisture profiles in the water vapor bands. Additionally, a microwave pressure sounder to detect backscattered signals at 60 GHz is outlined, along with lidar pressure and wind speed measurement methods.

Hinkley, E. D.↗

VLA observations of a complete sample of extragalactic X-ray sources. II

A complete sample of 35 X-ray selected sources found with the Einstein Observatory has been observed with the Very Large Array at 6 cm to investigate the relationship between radio and X-ray emission in extragalactic objects. Detections include three active galactic nuclei (AGNs), two clusters or groups of galaxies, two individual galaxies, and two BL Lac objects. The frequency of radio emission in X-ray selected AGNs is compared with that of optically selected quasars using the integral radio-optical luminosity function. The result suggests that the probability for X-ray selected quasars to be radio sources is higher than for those optically selected. No obvious correlation is found in the sample between the richness of X-ray luminosity of the cluster and the presence of a galaxy with radio luminosity at 5 GHz larger than 10 to the 30th ergs/s/Hz.

Schild, R.↗

Satellite detection of tropical burning in Brazil

Tropical burning often occurs in remote areas of the world. Satellite remote sensing is the only practical solution for detecting and monitoring this burning. The capability of the Advanced Very High Resolution Radiometer on board the National Oceanic and Atmospheric Administration polar orbiting satellites to detect tropical fire activity in the Manaus, Brazil area is demonstrated.

Matson, Michael↗

Lightning danger and advanced warning times obtainable from field mills

The rate of charge moment generation derived from Maxwell current densities at the ground is compared with the rate of charge moment removal by lightning for the initial stages of a small thunderstorm cell. It is shown that the current moment in the cell increased with time at a rate of 4.5 (A m/s) during the first seven minutes following detection of electrical activity on the ground. At the time of the first lightning flash which neutralized 0.48 (kC m) a charge moment of over 8 (kC m) had been separated. Theoretical and practical implications of these measurements are discussed.

Nisbet, John S.↗

Elementary bipoles of active regions and ephemeral active regions

The general properties of elementary bipoles (EBs), the class of moving magnetic features identified by Frazier (1972) as building blocks of new solar active regions, are described, and variations in their characteristics are illustrated with extensive videomagnetograms obtained at Big Bear Solar Observatory during 1984-1989. Consideration is given to ephemeral active regions consisting of EBs with only one positive and one negative pole, multiple-pole ephemeral regions, reversed-polarity EBs, interactions among EBs and adjacent magnetic features, and the EBs of small and medium active regions. The detection of EBs prior to the appearance of arch filaments confirms the relationship found by Frazier.

Martin, Sara F.↗

Chemical reactivity of the Martian soil

The Viking life sciences experimental packages detected extraordinary chemical activity in the martian soil, probably the result of soil-surface chemistry. At least one very strong oxidant may exist in the martian soil. The electrochemical nature of the martian soil has figured prominently in discussions of future life sciences research on Mars. Putative oxidants in the martian soil may be responsible for the destruction of organic material to considerable depth, precluding the recovery of reducing material that may be relic of early biological forms. Also, there have been serious expressions of concern regarding the effect that soil oxidants may have on human health and safety. The concern here has centered on the possible irritation of the respiratory system due to dust carried into the martian habitat through the air locks.

Zent, A. P.↗

Rapid Bacterial Testing for Spacecraft Water

Evaluations of the fluorogenic stains and probes will continue. E. coli 0157:H7 will be used as the reference strain for optimizing protocols. We anticipate the continued use of the fluorescent antibodies (TRITC and FITC labeled) in conjunction with CTC, Rhl23, DiBAC4(3), DAPI and acridine orange. Chemunex, the manufacturer of the ChemScan analyzer system, also makes a fluorogenic probe, Chemchrome B, which will be incorporated into the suite of probes to evaluate once their system is on site. Regardless of the combination of stains and probes all will be evaluated on membrane filters. Development of a FISH protocol that will be applicable to our conditions will be continued. Complimentary 16s rRNA probes to Ps. aeruginosa and currently in our laboratory will be evaluated first. Once this protocol has been adequately optimized other probes will be ordered for u a select number of other species. Currently, protocols to evaluate the effects of disinfection and the resulting lethality, injury on stain and/or probe specificity and reliability are being developed. E. coli 0157:H7 is the reference strain and chlorine the disinfectant the reference protocol is being developed around. Upon completion of this work, the resulting protocol will be extended to other species and disinfectants (e.g., iodine). Similar disinfectant experiments will then be conducted on the same species after starvation to evaluate the effects of starvation on disinfection resistance and the applicability of the stains and probes. Development of the immunomagnetic separation system will continue. Combined with the rapid methods described above, with enumeration by the ChemScan, we anticipate that this will provide a highly sensitive technique for the detection of specific, active bacteria.

Lisle, John T.↗

Juneau Airport Doppler Lidar Deployment: Extraction of Accurate Turbulent Wind Statistics

A 2 micrometer pulsed Doppler lidar was deployed to the Juneau Airport in 1998 to measure turbulence and wind shear in and around the departure and arrival corridors. The primary objective of the measurement program was to demonstrate and evaluate the capability of a pulsed coherent lidar to remotely and unambiguously measure wind turbulence. Lidar measurements were coordinated with flights of an instrumented research aircraft operated by representatives of the University of North Dakota (UND) under the direction of the National Center for Atmospheric Research (NCAR). The data collected is expected to aid both turbulence characterization as well as airborne turbulence detection algorithm development activities within NASA and the FAA. This paper presents a summary of the deployment and results of analysis and simulation which address important issues regarding the measurement requirements for accurate turbulent wind statistics extraction.

Hannon, Stephen M.↗