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NASA NTRSDate not supplied

Somatic Mutation in Mice on the International Space Station (ISS): Guanine Substitution Suggests Link to Cancer Risk

We conducted comprehensive analysis of single nucleotide somatic mutations in mice exposed to microgravity and other factors aboard the International Space Station (ISS), using data archived in GeneLab. Animals in the experimental cohort consisted of mice that spent 37 days on the ISS within the Rodent Habitat. Ground control animals consisted of mice of identical age, sex, strain, in a terrestrial Rodent Habitat controlled for temperature, humidity and carbon dioxide levels, to match ISS conditions as closely as possible. RNA extracted from eye, liver, skeletal muscle, and kidney tissue specimens was subjected to next-generation sequencing to acquire primary data. Our analysis employed cutting-edge software developed at NASA Ames Research Center, executed on the NASA Ames Supercomputer and on another high-performance computer, for accurate variant calling of single point mutations. ISS-flown mice exhibited a notably heightened level of somatic mutation compared to control mice. The degree of somatic mutation correlated with the degree of gene expression across the four tissue types, i.e., the greatest rate of mutation accumulation was seen in highly expressed genes. We discovered that guanine substitutions were the most common type of somatic mutation. This observation is consistent with the hypothesis that DNA mutation events stem from reactive oxygen/nitrogen/chlorine species-mediated guanine oxidation induced by the spaceflight environment. Since guanine oxidation is a prominent feature of the DNA mutation landscape that accompanies malignant transformation, our findings suggest a possible link between the spaceflight environment and cancer risk that is independent of radiation carcinogenesis.

ISS↗

NASA NTRS2012

ALSSAT Version 6.0

Advanced Life Support Sizing Analysis Tool (ALSSAT) at the time of this reporting has been updated to version 6.0. A previous version was described in Tool for Sizing Analysis of the Advanced Life Support System (MSC- 23506), NASA Tech Briefs, Vol. 29, No. 12 (December 2005), page 43. To recapitulate: ALSSAT is a computer program for sizing and analyzing designs of environmental-control and life-support systems for spacecraft and surface habitats to be involved in exploration of Mars and the Moon. Of particular interest for analysis by ALSSAT are conceptual designs of advanced life-support (ALS) subsystems that utilize physicochemical and biological processes to recycle air and water and process human wastes to reduce the need of resource resupply. ALSSAT is a means of investigating combinations of such subsystems technologies featuring various alternative conceptual designs and thereby assisting in determining which combination is most cost-effective. ALSSAT version 6.0 has been improved over previous versions in several respects, including the following additions: an interface for reading sizing data from an ALS database, computational models of a redundant regenerative CO2 and Moisture Removal Amine Swing Beds (CAMRAS) for CO2 removal, upgrade of the Temperature & Humidity Control's Common Cabin Air Assembly to a detailed sizing model, and upgrade of the Food-management subsystem.

Yeh, Hue-Hsia↗

NASA NTRS2021

Advanced Multimodal Solutions for Information Presentation

High-workload, fast-paced, and degraded sensory environments are the likeliest candidates to benefit from multimodal information presentation. For example, during extra-vehicular activity (EVA) and telerobotic operations, the sensory restrictions associated with such a hostile environment provide a major challenge to maintaining the situation awareness (SA) required for safe operations. In particular, orientation, navigation, and collision avoidance are critical aspects of EVA tasks that need to be addressed to ensure the safety of the crew and the success of the mission. Multimodal displays hold promise to enhance situation awareness and task performance by utilizing different sensory modalities and maximizing their effectiveness based on appropriate interaction between modalities. Multimodal displays will also play an important role for long-duration information systems and will likely begin to be developed in the early phases of cislunar Gateway, and later lunar or Mars transit missions. Information systems are envisioned for LDEMs that require spacecraft with greater crew autonomy and increased dependence on computer-provided information needed to perform routine tasks, as well as time- and safety critical tasks. Such a system will require a single, common interface that is easy to learn and use and accesses key information from all relevant vehicle/habitat systems to enable task performance in both nominal and emergency conditions. Understanding of multimodal display technologies and their interactions will help to inform interface guidelines for LDEMs. The scope of the current report is an analysis of potential multimodal display technologies for long duration missions and, in particular, will focus on their potential role in EVA activities. The review will address multimodal (combined visual, auditory and/or tactile) displays investigated by the National Aeronautics and Space Administration (NASA), industry, and Department of Defense (DoD). It also considers the need for adaptive information systems to accommodate a variety of operational contexts such as crew status (e.g., fatigue, workload level) and task environment (e.g., EVA, habitat, rover, spacecraft). Current approaches to guidelines and best practices for combining modalities for the most effective information displays are also reviewed. Potential issues in developing interface guidelines for LDEMs are briefly considered.

multimodal displays↗

NASA NTRSDate not supplied

The membrane bioreactor (MBR): A hybrid technology for bioregenerative wastewater treatment and resource recovery in space

Extraplanetary surface habitat life support systems (LSS) on the Moon and Mars, as well as long-duration space travel, will require novel capabilities to withstand anticipated unique, harsh conditions. In order to provide safe, habitable environments for the crew, water purification and waste processing systems will be required to treat all sources of water (condensate, Sabatier, urine, hygiene, fecal, food waste) in order to achieve the necessary levels of recovery needed to sustain life over the long-duration missions. The ability to recycle organic wastes creates an opportunity to recover critical elements (e.g., C, H, O, N, P) for subsequent food production, water purification, and atmospheric regeneration. Bioregenerative systems mimic functions of nature in engineered systems, or bioreactors, utilizing combination of prokaryotes, eukaryotes and archaea. While these systems are commonly used on Earth for wastewater treatment, bioreactors for space travel face additional challenges. Terrestrial bioreactors often rely on gravitational settling of dense flocs and granules for cell retention. For micro- or partial-gravity environments, density differential alone will not be adequate for cell retention; a gravity-independent means for cell retention is crucial. The membrane bioreactor represents the state of the art in wastewater treatment. This hybrid system combines biological processes with membrane filtration to achieve performance beyond what each can accomplish individually. The complete cell retention in an MBR allows for the decoupling of hydraulic retention time (HRT) and solids retention time (SRT), which result in a high-thruput, compact, treatment system. The Bioregenerative Water Technology Team at NASA Kennedy Space Center and the University of South Florida has developed a bioregenerative platform based on the hybrid MBR technology. The overall architecture is compact, modular, flexible, and adaptable to mission evolutions. The main subsystems of the bioregenerative architecture include: 1) Anaerobic membrane bioreactor (AnMBR): Also termed the Organic processor assembly (OPA), the function of the AnMBR is to treat organic wastes such as fecal and food wastes. These wastes are characterized by a concentration of suspended solids comprised of carbohydrates, proteins and lipids. The assigned function of the AnMBR is to break down and covert suspended solids to biogas (methane, hydrogen and carbon dioxide), reduce effluent chemical oxygen demand (COD), liberate organically-bound nutrients, and remove pathogenic organisms. 2) Phototrophic membrane bioreactor (PMBR): The PMBR is comprised of a co-culture of microalgae and bacteria. The assigned function of the PMBR is to polish the permeate of the AnMBR to further remove dissolved organic carbon, manage nutrients (nitrogen transformation, load dampening), and perform air revitalization. 3) Food processor assembly (FPA): The FPA is a food production platform (prokaryotic or eukaryotic), fueled by outputs from the AnMBR, or PMBR. For the presentation, we will describe each step of the bioregenerative architecture, and present performance data from extended trials treating analog and real metabolic wastes.

Bioreactor↗

DOE OSTI2025

Spatial Replication Is Important for Developing Landscape Genetic Inferences for a Wetland Salamander

Habitat fragmentation is a pressing threat to wildlife populations, and maintenance of gene flow between populations is an essential goal of conservation. Resistance surfaces have emerged as an important tool for modelling connectivity and developing management strategies to mitigate effects of habitat fragmentation. However, recent studies have noted inconsistencies in the factors most strongly associated with connectivity across different landscapes. Thus, replication of genetic-based resistance surface optimisation across landscapes may be necessary for making robust conclusions about the influence of environmental variables. Accordingly, replication represents a substantive challenge and opportunity in the field of landscape genetics. In this study, we conducted replicated landscape genetic analyses across five landscapes in Tennessee and Kentucky for a threatened wetland amphibian, the four-toed salamander (Hemidactylium scutatum). We tested multiple hypotheses of how different landscape features that could directly affect small, desiccation-intolerant amphibians (e.g., canopy cover) influenced gene flow and assessed the appropriate scale at which to model different features. We found some concordance in the landscape features that influenced gene flow (e.g., a common importance of forest cover and topography), but also some differences—potentially owing to the difference in variability of predictors across landscapes. We also found discordance in the scale of effect of different features across landscapes. In conclusion, our work emphasises that flat areas of moist forest not bisected by roads may be important for H. scutatum conservation, and our replicated design allows us to identify relationships that would have been missed if only using one study site.

59 BASIC BIOLOGICAL SCIENCES↗

NASA NTRS2011

Performance Comparison of Wireless Sensor Network Standard Protocols in an Aerospace Environment: ISA100.11a and ZigBee

Wireless Sensor Networks (WSNs) can provide a substantial benefit in spacecraft systems, reducing launch weight and providing unprecedented flexibility by allowing instrumentation capabilities to grow and change over time. Achieving data transport reliability on par with that of wired systems, however, can prove extremely challenging in practice. Fortunately, much progress has been made in developing standard WSN radio protocols for applications from non-critical home automation to mission-critical industrial process control. The relative performances of candidate protocols must be compared in representative aerospace environments, however, to determine their suitability for spaceflight applications. In this paper, we will present the results of a rigorous laboratory analysis of the performance of two standards-based, low power, low data rate WSN protocols: ZigBee Pro and ISA100.11a. Both are based on IEEE 802.15.4 and augment that standard's specifications to build complete, multi-hop networking stacks. ZigBee Pro targets primarily the home and office automation markets, providing an ad-hoc protocol that is computationally lightweight and easy to implement in inexpensive system-on-a-chip components. As a result of this simplicity, however, ZigBee Pro can be susceptible to radio frequency (RF) interference. ISA100.11a, on the other hand, targets the industrial process control market, providing a robust, centrally-managed protocol capable of tolerating a significant amount of RF interference. To achieve these gains, a coordinated channel hopping mechanism is employed, which entails a greater computational complexity than ZigBee and requires more sophisticated and costly hardware. To guide future aerospace deployments, we must understand how well these standards relatively perform in analog environments under expected operating conditions. Specifically, we are interested in evaluating goodput -- application level throughput -- in a representative crewed environment in the presence of varying levels of 802.11g Wi-Fi traffic. To do so, we use the NASA Johnson Space Center Wireless Habitat Testbed (WHT), a metallic, habitation-sized module designed for co-existence testing of wireless systems. In its quiescent state, the sealed WHT provides an RF-quiet environment to which we can selectively add interfering systems; it also provides a realistic level of multi-path self-interference for systems under investigation. In our test, we deploy two representative five node networks, configured in a star topology with all nodes reporting directly to a WSN gateway. Each ZigBee network WSN node is built using a Texas Instruments (TI) CC2530 system-on-a-chip radio running TI's ZigBee Pro Z-stack. Each ISA100.11a network node is built using a Nivis VersaNode 210 system-on-a-chip radio. In both cases, radios interface with TI MSP430-F5438 microcontroller implementing a common test application. Interference is provided by a D-link 802.11g Wi-Fi router transporting traffic generated using the Iperf network testing tool. For the single-channel ZigBee network, effects of both direct and indirect Wi-Fi interference are evaluated. For the channel-hopping ISA100.11a network, effects of interference from multiple Wi-Fi routers configured in non-overlapping 802.11g channels are evaluated. Our results show that, in general, the more lightweight ZigBee network performs well at low interference levels, but performance degrades as interference increases. Conversely, the more complex and costly ISA100.11a network continues to perform well as Wi-Fi interference levels increase.

Wagner, Raymond S.↗

NASA NTRS2018

In-Situ Navigation and Timing Services for a Human Mars Landing Site Part 2: System Design and Simulations

In a previous paper, we described the system concept of a proposed Mars Regional Navigation Satellite System (MRNSS) that is built upon a number of notional Mars orbiting and surface missions in the human exploration era of Mars. We assume two areostationary Mars relay orbiters that have continuous line-of-sight visibility with the Mars landing site, a Deep Space Habitat (DSH) in an inclined 48-hour circular orbit, and a surface communication lander that could serve as the reference point. These orbiting and surface infrastructure elements broadcast GPS-like ranging signals and other ephemeris information to the mission users. With one or more additional orbiters in areosynchronous orbits that trace around a figure-8 path, a regional navigation satellite system can be realized that provides in-situ course absolute localization and precision relative localization and timing services to the users in the vicinity of a Mars landing site. We also introduced a new geometric trilateration method that simultaneously performs absolute positioning and relative positioning. The relative position is derived from a “differencing” function of two raw-range measurements between a known reference point and of the target from a navigation satellite, thereby eliminating most of the common errors like atmospheric delays, ephemeris errors, and instrument delays in real-time. In the Mars environment, this “error-cancellation” function greatly reduces the need to perform extensive orbit determination (OD) of the navigation satellites like the Earth’s GPS, and only requires occasional tracks from the Earth’s large-aperture deep space antennas to perform OD’s. In this paper, we provide detailed simulations on both the absolute positioning scheme and the relative positioning scheme, and show that the relative positioning scheme provides 200 – 400 times improvement in localization accuracy. On the ground side, we discuss new approaches that simultaneously perform 2-way Doppler/ranging, and re-introduce the method of Same Beam Interferometry (SBI) for the Mars navigation orbiters. This helps to further reduce the tracking burden of the ground antennas required to support the OD of the Mars orbiters, making the concept of MRNSS more realizable.

Lightsey, Glenn↗

DOE OSTI2023

Scavenging dynamics on Guam and implications for invasive species management

Deployment of mouse carcasses laced with acetaminophen has become a common management tool to control invasive brown tree snakes (Boiga irregularis; BTS) on Guam. Additionally, anticoagulant rodenticides may be used to control invasive rats (Rattus spp.) if their populations increase due to predator release in the wake of BTS eradication. However, there has been little research examining how scavengers on Guam could be incidentally exposed to toxicants by scavenging carcasses of animals that die from these population control strategies. Furthermore, there is a limited understanding of how the proliferation of invasive species on Guam has influenced the composition of the scavenger community. We investigated these topics by examining scavenger consumption of mouse, rat, and BTS carcasses on Guam in both a coastal and upland site during the wet (May–Aug 2016) and dry season (Jan–Apr 2017). We documented carcass consumption by 9 species, which scavenged 48% of carcasses. Interactions between season, habitat, and carcass type influenced probability of scavenging, and appeared to be driven by consumption by the two main scavenger species, BTS and cane toads (Rhinella marina), both of which are invasive on Guam. Baiting programs should consider the potential for toxin exposure to land crabs (Coenobita spp., Birgus latro), native species that scavenged at every combination of carcass type, habitat, and season. Altogether, 60% of scavenging events were attributed to species considered pests that are recent introductions to Guam. Invasive species on Guam are the primary scavengers of small vertebrate carrion, suggesting a substantial role in trophic dynamics that extends beyond predation.

59 BASIC BIOLOGICAL SCIENCES↗

DOE OSTI2024

The impact of sampling scale: A comparison of methods for estimating external contaminant exposure in free-ranging wildlife

The impacts of contaminants on wildlife are dose dependent, and thus being able to track or predict exposure following contamination events is important for monitoring ecosystem health. However, the ability to track exposure in free-ranging wildlife is often severely limited. Consequently, researchers have predominantly relied on simple methods for estimating contaminant exposures in wildlife with little regard for spatial contaminant heterogeneity or an animal’s use of diverse habitats. We evaluated the influence sampling scale (i.e., how finely contaminant distribution and organism’s spatial use of the landscape is mapped) has on (1) realism and (2) conservativeness of exposure estimates. To do this, we monitored the actual exposure of wild boar (Sus scrofa) in Fukushima, Japan to radioactive contamination using GPS27 coupled contaminant monitors placed on individual animals. We compared empirical exposures to estimates generated by combining varying amounts of information about an individual boar’s location and/or movement, with the distribution of contamination on the landscape. Here we found that the most realistic exposure estimates were produced when finer-scale contaminant distribution surveys (e.g., airborne surveys) were combined with more accurate estimates of an individual’s space use (e.g., home ranges or core areas). Importantly, estimates of exposure based on single point surveys at a trap site (a simple method commonly used in the literature), did not correlate with actual exposure rates, suggesting dose-effects studies using this method may result in spurious conclusions. These results suggest that researchers seeking realistic estimates of exposure, such as in dose-effect studies, should ensure they have adequately accounted for fine-scale contaminant distribution patterns and areas of higher use by study organisms. However, conservative estimates of exposure i.e., intentionally over-predicting exposure as is done in initial tiers of ecological risk analyses) were not as scale sensitive and could be achieved with a single known location and coarse contaminant distribution maps.

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTAN↗

DOE OSTI2025

Genomic factors limiting the diversity of Saccharomycotina plant pathogens

The Saccharomycotina fungi have evolved to inhabit a vast diversity of habitats over their 400-million-year evolution. There are, however, only a few known fungal pathogens of plants in this subphylum, primarily belonging to the genera Eremothecium and Geotrichum. We compared the genomes of 12 plant-pathogenic Saccharomycotina strains to 360 plant-associated strains to identify features unique to the phytopathogens. Characterization of the oxylipin synthesis genes, a compound believed to be involved in Eremothecium pathogenicity, did not reveal any differences in gene presence within or between the plant-pathogenic and plant-associated strains. A reverse-ecological approach, however, revealed that plant pathogens lack several metabolic enzymes known to assist other phytopathogens in overcoming plant defenses. This includes L-rhamnose metabolism, formamidase and nitrilase genes. This result suggests that the Saccharomycotina plant pathogens are limited to infecting ripening fruits as they are without the necessary enzymes to degrade common phytohormones and secondary metabolites produced by plants.

Saccharomycotina, fungi, phytopathogen, reverse ec↗

NASA NTRS1977

Space Resources and Space Settlements

This publication contains the technical papers from the five task groups that took part in the 1977 Ames Summer Study on Space Settlements and Industrialization Using Nonterrestrial Materials. The study was sponsored by the following NASA Headquarters organizations: Office of Space Science, Office of Aeronautics and Space Technology, and Office of Space Transportation Systems. The NASA Ames Research Center hosted the study and provided administrative support through its Life Sciences Directorate. Expert assistance in the many aspects of the study came from NASA Headquarters, NASA centers, universities, industry, and from individual consultants. This summer study was the largest and most comprehensive investigation of space manufacturing and habitation to date; forty senior research workers and ten students participated. The six-week study was preceded by several months of planning and preparation, and was followed by extensive work on manuscript checking, review, and assembly. The study topics reflected the research areas requiring the most intensive attention as ofrnid-1977; they were: Group I: Regenerative life-support systems and controlled environment agriculture. Group II: Parametric studies of efficient habitats in space. Time and cost analysis of a space manufacturing program plan. Group III: Detection and analysis of special classes of asteroids suitable as material resources for space manufacturing; studies of retrieval missions. Group IV: Electromagnetic mass drivers for use as interorbital engines, as devices to launch lunar material into space, and as tugs for the retrieval of asteroidal material. Group V: Chemical processing of non terrestrial material in space; manufacture of metals and glass fibers, beneficiation, and design of processing plants. These five task groups each produced two or more technical papers, signed by the contributing members. The papers were subjected to independent peer review by scientists not connected with the study. During the course of the study, frequent formal and informal exchanges of information occurred between task groups, so that the entire effort evolved in a coherent fashion and was directed toward common goals. However, each group remained responsible for the choice of its topics and for its conclusions. A listing of all the participants is given on pages vii-x. It would be inappropriate for me to single out particular people or groups for special mention, since all involved applied their skills with dedication and enthusiasm. Instead, I thank everyone for their contributions to the success of the study, and also for making it a united and warmly cooperative personal experience.

John Billingham↗

NASA NTRS2009

Austere Human Missions to Mars

The Design Reference Architecture 5 (DRA 5) is the most recent concept developed by NASA to send humans to Mars in the 2030 time frame using Constellation Program elements. DRA 5 is optimized to meet a specific set of requirements that would provide for a robust exploration program to deliver a new six-person crew at each biennial Mars opportunity and provide for power and infrastructure to maintain a highly capable continuing human presence on Mars. This paper examines an alternate architecture that is scaled back from DRA 5 and might offer lower development cost, lower flight cost, and lower development risk. It is recognized that a mission set using this approach would not meet all the current Constellation Mars mission requirements; however, this 'austere' architecture may represent a minimum mission set that would be acceptable from a science and exploration standpoint. The austere approach is driven by a philosophy of minimizing high risk or high cost technology development and maximizing development and production commonality in order to achieve a program that could be sustained in a flat-funded budget environment. Key features that would enable a lower technology implementation are as follows: using a blunt-body entry vehicle having no deployable decelerators, utilizing aerobraking rather than aerocapture for placing the crewed element into low Mars orbit, avoiding the use of liquid hydrogen with its low temperature and large volume issues, using standard bipropellant propulsion for the landers and ascent vehicle, and using radioisotope surface power systems rather than a nuclear reactor or large area deployable solar arrays. Flat funding within the expected NASA budget for a sustained program could be facilitated by alternating cargo and crew launches for the biennial Mars opportunities. This would result in two assembled vehicles leaving Earth orbit for Mars per Mars opportunity. The first opportunity would send two cargo landers to the Mars surface to preposition a habitat, supplies, and exploration equipment. The next opportunity, two years later, would send to Mars orbit 1) a lander with a Mars Ascent Vehicle (MAV) and 2) a crewed Mars Transit Habitat with an Orion CEV for Earth return. The following opportunity, two years after the first crew, would go back to cargo-only launches. This alternation of cargo and crew opportunities results in a sustainable launch rate of six Ares V launches every two years. It is notable that four of the six launches per Mars opportunity are identical, build-to-print, Tran-Mars Injection stages. This type of production rate could lend itself well to a COTStype service provider, and would make it feasible to have a live spare in place in the event of a single launch failure.

landers↗

NASA NTRS2017

In-Situ Navigation and Timing Services for the Human Mars Landing Site Part 1: System Concept

In [1] and [2], we introduce a new geometric trilateration method that simultaneously performs absolute positioning and relative positioning. The relative position is derived from a “differencing” function of two raw-range measurements between a known reference point and of the target from a navigation satellite, thereby eliminating most of the common errors like atmospheric delays, ephemeris errors, and instrument delays in real-time. In the Mars environment this “error-cancellation” function greatly reduces the need to perform extensive orbit determination (OD) of the navigation satellites like the Earth’s GPS, and only requires occasional tracks from the Earth’s large-aperture deep space antennas to perform OD’s. Leveraging on this scheme, we propose a low-cost, low-maintenance regional navigation satellite system architecture that provides in-situ navigation and timing services for robotic and human missions in the vicinity of a Mars landing site. This architecture is built upon the proposed Mars relay network infrastructure, and a number of notional Mars orbiting and surface missions in the human exploration era of Mars. We assume two areostationary Mars relay orbiters that have continuous line-of-sight visibility with the Mars landing site, a Deep Space Habitat (DSH) in an inclined 48-hour circular orbit, and a surface communication lander that could serve as the reference point. These orbiting and surface infrastructure elements broadcast GPS-like ranging signals and other ephemeris information to the mission users. With one or more additional orbiters in areosynchronous orbits that trace around a figure-8 path, a regional navigation satellite system can be realized that provides in-situ course absolute localization and precision relative localization and timing services to the users in the vicinity of a Mars landing site. This paper describes the system concept of the proposed Mars regional navigation satellite system.

Lee, Charles↗

NASA NTRS2005

Valiant 'Zero-Valent' Effort Restores Contaminated Grounds

Dense non-aqueous phase liquids (DNAPLs) are chemical compounds that can contaminate soil and groundwater to the point of irreparability. These substances are only slightly soluble in water, and are much denser than water. Because of their solubility, DNAPLs form separate liquid phases in groundwater, and because of their density, DNAPLs sink in aquifers instead of floating at the water table, making it extremely difficult to detect their presence. If left untreated in the ground, they can taint fresh water sources. Common DNAPLs include chlorinated hydrocarbon compounds such as carbon tetrachloride, chloroform, tetrachloroethylene, and trichloroethylene. Trichloroethylene was used during the early days of the Space Program, as a solvent for flushing rocket engines, and for metal cleaning and degreasing of equipment, electronics, and heavy machinery. As a result, areas of Cape Canaveral s Launch Complex 34, the site of several historic Saturn rocket launches occurring from 1959 to 1968, were polluted with chlorinated DNAPLs. Through the direction and guidance of Dr. Jacqueline Quinn, an environmental engineer in the Spaceport Engineering and Technology Directorate at NASA s Kennedy Space Center, a biodegradable environmental cleanup technology was developed to reductively dechlorinate DNAPL sources in polluted water at Launch Complex 34. It was important for Kennedy to nip this problem in the bud, in light of the fact that the Space Center is also a National Wildlife Refuge, home to thousands of shorebirds, endangered sea turtles and eagles, manatees, alligators, and diverse habitats that include brackish marshes and salt water estuaries. The success in remediating this historic launch site has led to numerous commercial applications that are restoring the health of our environmental surroundings.

Source record↗

NASA NTRS2017

Mouse Behavior on ISS: The Emergence of Distinctive, Organized Group Circling Behavior Unique to Spaceflight

As interest in long duration effects of space habitation increases, understanding the behavior of model organisms living within the habitats engineered to fly them is vital for designing, validating, and interpreting future spaceflight studies. Only a handful of papers have previously reported behavior of mice and rats in the weightless environment of space (Andreev-Andrievskiy, et al., 2013; Cancedda et al., 2012; Ronca et al., 2008). The Rodent Research Hardware and Operations Validation Mission (Rodent Research-1; RR1) utilized the Rodent Habitat (RH) developed at NASA Ames Research Center to fly mice on the ISS. Ten adult (16-week-old) female C57BL6J mice were launched on September 21st, 2014 in an unmanned Dragon Capsule, and spent 37 days in flight. Here we report group behavioral phenotypes of the RR1 Flight (FLT) and environment-matched Ground Control (GC) mice in the RH during this long duration flight. Video was recorded for 34 days on the ISS, permitting daily assessments of overall health and well being of the mice, and providing a valuable repository for detailed behavioral analysis. As compared to GC mice, RR1 FLT mice exhibited the same range of behaviors, including eating, drinking, exploration, self- and allogrooming,and social interactions at similar or greater levels of occurrence. Overallactivity was greater in FLT as compared to GC mice, with spontaneous ambulatory behavior, including organized circling or race-tracking behavior that emerged within thefirst few days of flight following a common developmental sequence, comprising theprimary dark cycle activity of FLT mice. Circling participation by individual micepersisted throughout the mission. Analysis of group behavior over mission days revealed recruitment of mice into the group phenotype, coupled with decreasing numbers of collisions between circling mice. This analysis provides insights into the behavior of mice in microgravity, and clear evidence for the emergence of a distinctive,organized group behavior unique to the weightless space environment.

mice↗

NASA NTRS2017

Mouse Behavior on ISS: The Emergence of a Distinctive, Organized Group Circling Behavior Unique to Spaceflight

As interest in long duration effects of space habitation increases, understanding the behavior of model organisms living within the habitats engineered to fly them is vital for designing, validating, and interpreting future spaceflight studies. Only a handful of papers have previously reported behavior of mice and rats in the weightless environment of space (Andreev-Andrievskiy, et al., 2013; Cancedda et al., 2012; Ronca et al., 2008). The Rodent Research Hardware and Operations Validation Mission (Rodent Research-1; RR1) utilized the Rodent Habitat (RH) developed at NASA Ames Research Center to fly mice on the ISS. Ten adult (16-week-old) female C57BL6J mice were launched on September 21st, 2014 in an unmanned Dragon Capsule, and spent 37 days in flight. Here we report group behavioral phenotypes of the RR1 Flight (FLT) and environment-matched Ground Control (GC) mice in the RH during this long duration flight. Video was recorded for 34 days on the ISS, permitting daily assessments of overall health and well being of the mice, and providing a valuable repository for detailed behavioral analysis. As compared to GC mice, RR1 FLT mice exhibited the same range of behaviors, including eating, drinking, exploration, self- and allogrooming, and social interactions at similar or greater levels of occurrence. Overall activity was greater in FLT as compared to GC mice, with spontaneous ambulatory behavior, including organized circling or race-tracking behavior that emerged within the first few days of flight following a common developmental sequence, comprising the primary dark cycle activity of FLT mice. Circling participation by individual mice persisted throughout the mission. Analysis of group behavior over mission days revealed recruitment of mice into the group phenotype, coupled with decreasing numbers of collisions between circling mice. This analysis provides insights into the behavior of mice in microgravity, and clear evidence for the emergence of a distinctive, organized group behavior unique to the weightless space environment. Supported by the NASA Rodent Research Project, Space Biology Program, and Space Life Sciences Training Program.

spaceflight↗

NASA NTRSDate not supplied

Crew Performance Support System to Aid in Anomaly Resolution: Concept of Operations

As missions progress into deep space, communication delays and disruptions will disenable the crew’s reliance on Earth experts. There are also limitations in the amount of data that can be downlinked to the ground. It is prudent to assume that critical, complex vehicle or habitat sub-systems will malfunction at a time when a lunar or Mars’ crew cannot rely on the Earth-Support team to detect, diagnose and resolve the problem and it is impractical to expect a small crew to step-in with the same level of expertise as 50+ authorities. The crew will need novel processes and advanced technological support to independently identify and resolve safety- and time-critical anomalies. That a self-reliant crew is unable to respond appropriately to time-critical anomalies is a significant risk to crew safety and mission success. This risk is driven by several factors; novel and unanticipated anomalies would not have been trained pre-flight, the crew could forget their pre-flight training or spaceflight stressors could impair the crew’s problem-solving ability. At last year’s IWS, Beard reported that a single spaceflight stressor (elevated CO2) could undermine the crew’s ability to independently respond to emergencies. Concept of Operations (ConOps) provide a common view of future system functions to all stakeholders. For the current project, a ConOps was developed that describes the operational processes, practices and capabilities needed by a crew of astronauts on deep space missions to autonomously respond to anticipated and unanticipated anomalies. It is crucial to recognize that, as of August 2018 existing technologies are unable to effectively support crew anomaly response to unanticipated events. “Intelligent technology” has not reached a maturity level that permits generalizing a solution to novel situations. For example, to train intelligent technology requires volumes of data that do not exist. The complexities involved in a manned mission to Mars cannot be compared to sending rovers to Mars using scripted software. This ConOps proposes a Crew Performance Support System (CPSS) that will push NASA and its industry partners toward what will be required for a safe and successful manned mission to Mars. Anomaly resolution during a deep space mission will take place within a dynamic, or changing, context. The figure to the left shows five broad contextual variables: the organizational culture, mission context, system characteristics, team characteristics and individual characteristics. The yellow arrow indicates that spaceflight and task-related stressors can affect system, team and individual crewmember characteristics and therefore anomaly response potential. The figure depicts a protective umbrella of Human-System Integration (HSI) principles that should be instituted during CPSS development including a balanced workload, shared situation awareness and building an appropriate level of trust in the automation. The figure also depicts two interrelated and cooperative components, an HSI Data System and other Enabling Capabilities will be required to support crew anomaly response and Earth-Support situation awareness. As we journey from ISS to Gateway to Mars, multiple, simultaneous and integrated research and development efforts (i.e., support systems co-evolution) must be implemented to meet the problem-solving challenges a self-reliant crew will face on a Mars’ mission. The crossovers between the capabilities are just as important as the discrete capabilities themselves. As the capabilities mature, the lines between the support subdomains will blur and an integrated system will emerge. The ConOps summarizes current knowledge about how highly trained people solve anomalies in safety- and time-critical situations, describes a group of capabilities that could help to reduce the extant risk and documents requirements levied on additional systems that provides critical inputs to the CPSS. Scenarios are used to promote a shared understanding of processes, practices and technological goals needed for safe and productive manned missions beyond LEO.

HSIA risk↗

DOE OSTI2020

Genomic Analysis Enlightens Agaricales Lifestyle Evolution and Increasing Peroxidase Diversity

Abstract As actors of global carbon cycle, Agaricomycetes (Basidiomycota) have developed complex enzymatic machineries that allow them to decompose all plant polymers, including lignin. Among them, saprotrophic Agaricales are characterized by an unparalleled diversity of habitats and lifestyles. Comparative analysis of 52 Agaricomycetes genomes (14 of them sequenced de novo) reveals that Agaricales possess a large diversity of hydrolytic and oxidative enzymes for lignocellulose decay. Based on the gene families with the predicted highest evolutionary rates—namely cellulose-binding CBM1, glycoside hydrolase GH43, lytic polysaccharide monooxygenase AA9, class-II peroxidases, glucose–methanol–choline oxidase/dehydrogenases, laccases, and unspecific peroxygenases—we reconstructed the lifestyles of the ancestors that led to the extant lignocellulose-decomposing Agaricomycetes. The changes in the enzymatic toolkit of ancestral Agaricales are correlated with the evolution of their ability to grow not only on wood but also on leaf litter and decayed wood, with grass-litter decomposers as the most recent eco-physiological group. In this context, the above families were analyzed in detail in connection with lifestyle diversity. Peroxidases appear as a central component of the enzymatic toolkit of saprotrophic Agaricomycetes, consistent with their essential role in lignin degradation and high evolutionary rates. This includes not only expansions/losses in peroxidase genes common to other basidiomycetes but also the widespread presence in Agaricales (and Russulales) of new peroxidases types not found in wood-rotting Polyporales, and other Agaricomycetes orders. Therefore, we analyzed the peroxidase evolution in Agaricomycetes by ancestral-sequence reconstruction revealing several major evolutionary pathways and mapped the appearance of the different enzyme types in a time-calibrated species tree.

59 BASIC BIOLOGICAL SCIENCES↗