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At least 253 records · Page 14

Gamma-aminobutyric acid (GABA) and neuropeptides in neural areas mediating motion-induced emesis

Immunocytochemical methods were employed to localize the neurotransmitter amino acid gamma-aminobutyric acid and the neuropeptides substance P and Met-enkephalin in the area postrema (AP), area subpostrema (ASP), nucleus of the tractus solitarius (NTS), dorsal motor nucleus of the vagus nerve (DMNV), and lateral vestibular nucleus (LVN). Glutamic acid decarboxylase immunoreactive (GAD-IR) terminals and fibers were observed in the AP and particularly in the ASP. A gradual decrease in the density of terminals was seen towards the solitary complex. The DMNV revealed irregularly scattered GAD-IR terminals within the neuropil or closely surrounding neuronal cell bodies. The LVN, particularly the dorsal division, showed numerous axon terminals which were mostly localize around large neurons and their proximal dendrites. Substance P immunoreactive (SP-IR) terminals and fibers showed high density in the solitary complex, in particular within the lateral division. The ASP showed medium to low density of SP-IR fibers and terminals. The AP exhibited a small number of fibers and terminals irregularly distributed. The DMNV revealed a high density of SP-IR terminals and fibers that were mainly concentrated in the periphery. Very few terminals were detected in the LVN. Met-enkephalin immunoreactive (Met-Enk-IR) fibers and terminals showed high density and uniform distribution in the DMNV. Scattered terminals and fibers were observed in the AP, ASP, and NTS (particularly the lateral division). The very few fibers were observed in the LVN surrounded the neuronal cell bodies. The present report is part of a study designed to investigate the interaction between neuropeptides and conventional neurotransmitters under conditions producing motion sickness and in the process of sensory-motor adaptation.

F Damelio↗

Interactive, Secure Web-enabled Aircraft Engine Simulation Using XML Databinding Integration

This paper discusses the detailed design of an XML databinding framework for aircraft engine simulation. The framework provides an object interface to access and use engine data. while at the same time preserving the meaning of the original data. The Language independent representation of engine component data enables users to move around XML data using HTTP through disparate networks. The application of this framework is demonstrated via a web-based turbofan propulsion system simulation using the World Wide Web (WWW). A Java Servlet based web component architecture is used for rendering XML engine data into HTML format and dealing with input events from the user, which allows users to interact with simulation data from a web browser. The simulation data can also be saved to a local disk for archiving or to restart the simulation at a later time.

Lin, Risheng↗

VERDEX: A virtual environment demonstrator for remote driving applications

One of the key areas of the National Advanced Robotics Centre's enabling technologies research program is that of the human system interface, phase 1 of which started in July 1989 and is currently addressing the potential of virtual environments to permit intuitive and natural interactions between a human operator and a remote robotic vehicle. The aim of the first 12 months of this program (to September, 1990) is to develop a virtual human-interface demonstrator for use later as a test bed for human factors experimentation. This presentation will describe the current state of development of the test bed, and will outline some human factors issues and problems for more general discussion. In brief, the virtual telepresence system for remote driving has been designed to take the following form. The human operator will be provided with a helmet-mounted stereo display assembly, facilities for speech recognition and synthesis (using the Marconi Macrospeak system), and a VPL DataGlove Model 2 unit. The vehicle to be used for the purposes of remote driving is a Cybermotion Navmaster K2A system, which will be equipped with a stereo camera and microphone pair, mounted on a motorized high-speed pan-and-tilt head incorporating a closed-loop laser ranging sensor for camera convergence control (currently under contractual development). It will be possible to relay information to and from the vehicle and sensory system via an umbilical or RF link. The aim is to develop an interactive audio-visual display system capable of presenting combined stereo TV pictures and virtual graphics windows, the latter featuring control representations appropriate for vehicle driving and interaction using a graphical 'hand,' slaved to the flex and tracking sensors of the DataGlove and an additional helmet-mounted Polhemus IsoTrack sensor. Developments planned for the virtual environment test bed include transfer of operator control between remote driving and remote manipulation, dexterous end effector integration, virtual force and tactile sensing (also the focus of a current ARRL contract, initially employing a 14-pneumatic bladder glove attachment), and sensor-driven world modeling for total virtual environment generation and operator-assistance in remote scene interrogation.

Stone, Robert J.↗

A finite-element perturbation approach to fluid/rotor interaction in turbomachinery elements. I - Theory. II - Application

The vibrational characteristics of a rotor that is in contact with a fluid in an annular clearance gap are investigated. The disturbance under consideration involves the axis of rotation, and includes a virtual lateral eccentricity, together with a whirling motion around the housing centerline. The fluid reaction components arise from infinitesimally small deformations with varied magnitudes which are experienced by an assembly of finite elements in the rotor-to-housing gap. A perturbation model is presented in which the perturbation equations emerge from the flow-governing equations in their discrete finite-element form. It is concluded that restrictions on the rotor-to-housing gap geometry, or the manner in which the rotor virtual eccentricity occurs are practically nonexisting. This model is used to compute the rotordynamic coefficients of an annular seal. The rotordynamic behavior of a hydraulic seal with a clearance gap depth/length ratio of 0.01 is analyzed under a cylindrical type of rotor whirl and several running speeds.

Baskharone, E. A.↗

Heat transfer and film cooling following injection through inclined circular tubes

Film cooling effectiveness and heat transfer are measured downstream of injection through discrete holes into a turbulent mainstream boundary layer. Air is injected through both a single hole and a row of holes spaced at three-diameter intervals and inclined at an angle of 35 deg to the main flow. There is little difference between the heat transfer coefficient with blowing and without blowing at low blowing rates (mass flux ratios). In fact, at low blowing rates, injection is found to decrease somewhat the heat transfer coefficient from that measured without blowing. As the mass flux ratio increases past unity, the heat transfer coefficient increases, especially with injection through a row of holes. The peak heat transfer is usually found at the edge of the spreading jets (i.e., between two holes). At a blowing rate near two, the lateral average of the heat transfer is as much as 27 percent higher than the heat transfer with no blowing. The increase in heat transfer is attributed to the interaction between the jets and the free stream, causing high levels of turbulence.

Eriksen, V. L.↗

A study of the dynamics of the Intertropical Convergence Zone (ITCZ) in a symmetric atmosphere-ocean model

A numerical model of the circulation of a coupled axisymmetric atmosphere-ocean system was constructed to investigate the physical factors governing the location and intensity of the Intertropical Convergence Zone (ITCZ) over oceans and over land. The results of several numerical integrations are presented to illustrate the interaction of the individual atmospheric and oceanic circulations. It is shown that the ITCA cannot be located at the equator because the atmosphere-ocean system is unstable for lateral displacements of the ITCA from an equilibrium position at the equator.

Charney, J. G.↗

Development of an Aeroelastic Modeling Capability for Transient Nozzle Side Load Analysis

Lateral nozzle forces are known to cause severe structural damage to any new rocket engine in development. Currently there is no fully coupled computational tool to analyze this fluid/structure interaction process. The objective of this study was to develop a fully coupled aeroelastic modeling capability to describe the fluid/structure interaction process during the transient nozzle operations. The aeroelastic model composes of three components: the computational fluid dynamics component based on an unstructured-grid, pressure-based computational fluid dynamics formulation, the computational structural dynamics component developed in the framework of modal analysis, and the fluid-structural interface component. The developed aeroelastic model was applied to the transient nozzle startup process of the Space Shuttle Main Engine at sea level. The computed nozzle side loads and the axial nozzle wall pressure profiles from the aeroelastic nozzle are compared with those of the published rigid nozzle results, and the impact of the fluid/structure interaction on nozzle side loads is interrogated and presented.

Wang, Ten-See↗

Laboratory-Model Integrated-System FARAD Thruster

Pulsed inductive plasma accelerators are spacecraft propulsion devices in which energy is stored in a capacitor and then discharged through an inductive coil. The device is electrodeless, inducing a plasma current sheet in propellant located near the face of the coil. The propellant is accelerated and expelled at a high exhaust velocity (order of 10 km/s) through the interaction of the plasma current with an induced magnetic field. The Faraday Accelerator with RF-Assisted Discharge (FARAD) thruster [1,2] is a type of pulsed inductive plasma accelerator in which the plasma is preionized by a mechanism separate from that used to form the current sheet and accelerate the gas. Employing a separate preionization mechanism in this manner allows for the formation of an inductive current sheet at much lower discharge energies and voltages than those found in previous pulsed inductive accelerators like the Pulsed Inductive Thruster (PIT). In a previous paper [3], the authors presented a basic design for a 100 J/pulse FARAD laboratory-version thruster. The design was based upon guidelines and performance scaling parameters presented in Refs. [4, 5]. In this paper, we expand upon the design presented in Ref. [3] by presenting a fully-assembled and operational FARAD laboratory-model thruster and addressing system and subsystem-integration issues (concerning mass injection, preionization, and acceleration) that arose during assembly. Experimental data quantifying the operation of this thruster, including detailed internal plasma measurements, are presented by the authors in a companion paper [6]. The thruster operates by first injecting neutral gas over the face of a flat, inductive acceleration coil and at some later time preionizing the gas. Once the gas is preionized current is passed through the acceleration coil, inducing a plasma current sheet in the propellant that is accelerated away from the coil through electromagnetic interaction with the time-varying magnetic field. Neutral gas is injected over the face of the acceleration coil through a fast-acting valve that feeds a central distribution manifold. The thruster is designed to preionize the gas using an RF-frequency ringing signal produced by a discharging Vector Inversion Generator (VIG). The acceleration stage consists of a multiple-turn, multiple-strand spiral induction coil (see Fig. 1, left panel) and is designed for operation at discharge energies on the order of 100 J/pulse. Several different pulsed power train modules can be used to drive current through the acceleration coil. One such power train is based upon the Bernardes and Merryman circuit topology, which restricts voltage reversal on the capacitor banks and can be clamped to eliminate current reversal in the coil. A second option is a pulse-compression-ring power train (see Fig. 1, right panel), which takesa temporally broad, low current pulse and transforms it into a short, high current pulse.

Polzin, K.A.↗

A tool for interactive exploration of a hierarchical segmentation

Hierarchical segmentation is discussed as a form of region growing in which the sequence of merges is controlled by a 'best merge first' principle, and a record of the region merging sequence is often retained for later analysis. In the author's massively parallel implementation of hierarchical segmentation, which he calls iterative parallel region growing (IPRG), a set of directional edge maps are used to store the region merging sequence information. An iteractive tool is described that allows an analyst to fully explore a hierarchical segmentation, potentially producing an image segmentation that is a combination of segmentations produced at several different iterations of the IPRG algorithm. A potential method for automatically producing such segmentations is also discussed.

Tilton, James C.↗

Three-Dimensional Navier-Stokes Simulations with Two-Equation Turbulence Models of Intersecting Shock-Waves/Turbulent Boundary Layer at Mach 8.3

An investigation of the numerical simulation with two-equation turbulence models of a three-dimensional hypersonic intersecting (SWTBL) shock-wave/turbulent boundary layer interaction flow is presented. The flows are solved with an efficient implicit upwind flux-difference split Reynolds-averaged Navier-Stokes code. Numerical results are compared with experimental data for a flow at Mach 8.28 and Reynolds number 5.3x10(exp 6) with crossing shock-waves and expansion fans generated by two lateral 15 fins located on top of a cold-wall plate. This experiment belongs to the hypersonic database for modeling validation. Simulations show the development of two primary counter-rotating cross-flow vortices and secondary turbulent structures under the main vortices and in each corner singularity inside the turbulent boundary layer. A significant loss of total pressure is produced by the complex interaction between the main vortices and the uplifted jet stream of the boundary layer. The overall agreement between computational and experimental data is generally good. The turbulence modeling corrections show improvements in the predictions of surface heat transfer distribution and an increase in the strength of the cross-flow vortices. Accurate predictions of the outflow flowfield is found to require accurate modeling of the laminar/turbulent boundary layers on the fin walls.

Bardina, J. E.↗

Global dynamic modeling of a transmission system

The work performed on global dynamic simulation and noise correlation of gear transmission systems at the University of Akron is outlined. The objective is to develop a comprehensive procedure to simulate the dynamics of the gear transmission system coupled with the effects of gear box vibrations. The developed numerical model is benchmarked with results from experimental tests at NASA Lewis Research Center. The modal synthesis approach is used to develop the global transient vibration analysis procedure used in the model. Modal dynamic characteristics of the rotor-gear-bearing system are calculated by the matrix transfer method while those of the gear box are evaluated by the finite element method (NASTRAN). A three-dimensional, axial-lateral coupled bearing model is used to couple the rotor vibrations with the gear box motion. The vibrations between the individual rotor systems are coupled through the nonlinear gear mesh interactions. The global equations of motion are solved in modal coordinates and the transient vibration of the system is evaluated by a variable time-stepping integration scheme. The relationship between housing vibration and resulting noise of the gear transmission system is generated by linear transfer functions using experimental data. A nonlinear relationship of the noise components to the fundamental mesh frequency is developed using the hypercoherence function. The numerically simulated vibrations and predicted noise of the gear transmission system are compared with the experimental results from the gear noise test rig at NASA Lewis Research Center. Results of the comparison indicate that the global dynamic model developed can accurately simulate the dynamics of a gear transmission system.

Choy, F. K.↗

Gas in merging galaxies

We present observations of the neutral hydrogen, ionized hydrogen, and starlight of galaxies chosen from the 'Toomre Sequence' of merging galaxies. This sequence is meant to represent the progressive stages of the merger of two disk galaxies into a single elliptical-like remnant. The galaxies in this study span the full range of this sequence. The stars and atomic gas are very differently distributed, with the stars more widely distributed at early stages, and the gas much more widely distributed at later stages. Large quantities of neutral gas are sent to large radii (greater than or approximately equal to 100 h(exp -1) kpc), and still persist even after the central remnant has relaxed to an r(sup 1/4) light profile. There are a few times 10(exp 9) solar masses h(exp -2) of both molecular and atomic gas in each of these systems. Throughout the different stages, about half of the total gas mass lies within the galaxies' optical bodies. The fraction of this mass that is in neutral hydrogen drops rapidly in the later stage mergers, suggesting that atomic gas is processed into molecular gas, stars, and hot gas during the merger and resulting starburst. Star formation occurs at all stages of the interaction, both within the tails and in the central bodies. In the early stages, the H(alpha) shows many arcs and plumes. In the late stages, there are large H 2 regions in the tails which are associated with large quantities of neutral hydrogen. There is always a very good correlation between optical, H(alpha), and H1 peaks, with N(sub H1) greater than or approximately equal 3 x 10(exp 20) cm(exp -2) at the location of the H2 regions in the tails.

Hibbard, John E.↗

Technology transfer within the government

The report of a workshop panel concerned with technology transfer within the government is presented. The suggested subtopics for the panel were as follows: (1) transfer from non-NASA U.S. government technology developers to NASA space missions/programs; and (2) transfer from NASA to other U.S. government civil space mission programs. Two presentations were made to the panel: Roles/Value of Early Strategic Planning Within the Space Exploration Initiative (SEI) to Facilitate Later Technology Transfer To and From Industry; and NOAA Satellite Programs and Technology Requirements. The panel discussion addresses the following major issues: DOD/NASA cooperation; alternative mechanisms for interagency communication and interactions; current technology transfer relationships among federal research agencies, and strategies for improving this transfer; technology transfer mechanisms appropriate to intragovernment transfer; the importance of industry as a technology transfer conduit; and measures of merit.

Christensen, Carissa Bryce↗

Heliosheath Space Environment Interactions with Icy Bodies in the Outermost Solar System

The Voyager 1 and 2 spacecraft are exploring the space environment of the outermost solar system at the same time that earth-based astronomy continues to discover new icy bodies, one larger than Pluto, in the transitional region outward from the Classical Kuiper Belt to the Inner Oort Cloud. Some of the Scattered Disk Objects in this region periodically pass through the heliosheath, entered by Voyager 1 in Dec. 2004 and later expected to be reached by Voyager 2, and out even beyond the heliopause into the Very Local Interstellar Medium. The less energetic heliosheath ions, important for implantation and sputtering processes, are abundant near and beyond the termination shock inner boundary, but the source region of the more penetrating anomalous cosmic ray component has not yet been found. Advantageous for modeling of icy body interactions, the measured heliosheath flux spectra are relatively more stable within this new regime of isotropic compressional magnetic turbulence than in the upstream heliospheric environment. The deepest interactions and resultant radiation-induced chemistry arise from the inwardly diffusing component of the galactic cosmic ray ions with significant intensity modulation also arising in the heliosheath beyond Voyager 1. Surface gardening by high-velocity impacts of smaller bodies (e.g., fragments of previous KBO collisions) and dust is a further space weathering process setting the time scales for long term exposure of different regolith layers to the ion irradiation. Sputtering and ionization of impact ejecta grains may provide a substantial feedback of pickup ions for multiple cycles of heliosheath acceleration and icy body interaction. Thus the space weathering interactions are potentially of interest not only for effects on sensible surface composition of the icy bodies but also for evolution of the heliosheath plasma energetic ion, and neutral emission environment.

Cooper, John F.↗

Interaction of Light and Ethylene on Stem Gravitropism

The major objective of this study was to evaluate light-regulated ethylene production during gravitropic bending in etiolated pea stems. Previous investigations indicated that ethylene production increases after gravistimulation and is associated with the later (counter-reactive) phase of bending. Additionally, changes in the counter-reaction and locus of curvature during gravitropism are greatly influenced by red light and ethylene production. Ethylene production may be regulated by the levels of available precursor (1-aminocyclopropane-l-carboxylic acid, ACC) via its synthesis, conjugation to malonyl-ACC or glutamyl-ACC, or oxidation to ethylene. The regulation of ethylene production by quantifying ACC and conjugated ACC levels in gravistimulated pea stemswas examined. Also measured was the changes in protein and enzyme activity associated with gravitropic curvature by electrophoretic and spectrophotometric techniques. An image analysis system was used to visualize and quantify enzymatic activity and transcriptional products in gravistimulated and red-light treated etiolated pea stem tissues.

Harrison, Marcia A.↗

Poxvirus infection triggers remodeling of host m⁶A epitranscriptome and benefits from the m⁶A regulatory responses

Understanding how host gene regulation responds to viral infection is essential for developing effective antiviral strategies. Emerging evidence suggests that host transcripts undergo dynamic chemical modifications to counteract viral invasion. Conversely, viruses that rely on nuclear transcription exploit host RNA methyltransferases to enhance mRNA export and translation. Orthopoxviruses, however, complete their entire replication cycle within compartmentalized cytoplasmic “factories” utilizing enzymes encoded by their large double-stranded viral DNA genomes. The dynamic interplay between host and poxviral epitranscriptome remains poorly characterized. Using a temporally resolved model of Vaccinia virus (VV) infection, we investigated host-virus interactions through transcriptome and N6-methyladenosine (m⁶A) epitranscriptome whole genome sequencing. We found that host m⁶A modifications respond rapidly to VV infection, preceding the delayed transcriptional changes that emerge at later stages. Early m⁶A signatures included key innate immunity factors as well as host genes involved in transcriptional regulation, post-transcriptional modification, and protein ubiquitination. Functional assays validated two host factors with early m⁶A modification changes that are essential for VV infection: a m⁶A reader, YTHDF1, and a component of the SCF E3 ubiquitin ligase complex, FBXO31. The m⁶A gain on YTHDF1 enhanced its protein expression and promoted efficient VV replication. In addition, we identified previously unrecognized roles of FBXO31 and the SCF E3 ligase complex in supporting VV infection. Temporal profiling of the m⁶A epitranscriptome reveals how VV exploits host post-transcriptional regulatory pathways, specifically m⁶A RNA modification and protein ubiquitination. These findings highlight critical host factors co-opted during poxvirus infection and identify potential targets for therapeutic intervention.

59 BASIC BIOLOGICAL SCIENCES↗

Real-time hierarchically distributed processing network interaction simulation

The Telerobot Testbed is a hierarchically distributed processing system which is linked together through a standard, commercial Ethernet. Standard Ethernet systems are primarily designed to manage non-real-time information transfer. Therefore, collisions on the net (i.e., two or more sources attempting to send data at the same time) are managed by randomly rescheduling one of the sources to retransmit at a later time interval. Although acceptable for transmitting noncritical data such as mail, this particular feature is unacceptable for real-time hierarchical command and control systems such as the Telerobot. Data transfer and scheduling simulations, such as token ring, offer solutions to collision management, but do not appropriately characterize real-time data transfer/interactions for robotic systems. Therefore, models like these do not provide a viable simulation environment for understanding real-time network loading. A real-time network loading model is being developed which allows processor-to-processor interactions to be simulated, collisions (and respective probabilities) to be logged, collision-prone areas to be identified, and network control variable adjustments to be reentered as a means of examining and reducing collision-prone regimes that occur in the process of simulating a complete task sequence.

Zimmerman, W. F.↗

Energetic Ion Interactions with the Galilean Satellites

The principal research tasks of this investigation are: (1) specification of the energetic (keV to MeV) ion environments upstream of the four Galilean satellites and (2) data analysis and numerical modeling of observed ion interactions with the satellites. Differential flux spectra are being compiled for the most abundant ions (protons, oxygen, and sulfur) from measurements at 20 keV to 100 MeV total energy by the Energetic Particle Detector (EPD) experiment and at higher ion energies by the Heavy Ion Counter (HIC) experiment. Runge-Kutta and other numerical techniques are used to propagate test particles sampled from the measured upstream spectra to the satellite surface or spacecraft through the local magnetic and corotational electric field environment of each satellite. Modeling of spatial variations in directional flux anisotropies measured during each close flyby provides limits on atomic charge states for heavy (O, S) magnetospheric ions and on internal or induced magnetic fields of the satellites. Validation of models for magnetic and electric field configurations then allows computation of rates for ion implantation, sputtering, and energy deposition into the satellite surfaces for further modeling of observable chemical changes induced by irradiation. Our ongoing work on production of oxidants and other secondary species by ice irradiation on Europa's surface has significant applications, already acknowledged in current literature, to astrobiological evolution. Finally, the work will improve understanding of energetic ion sources and sinks at the satellite orbits for improved modeling of magnetospheric transport processes. The scope of the research effort mainly includes data from the primary Galileo mission (1995-1997) but may also include some later data where directly relevant (e.g., comparison of J0 and I27 data for Io) to the primary mission objectives. Funding for this contract also includes partial support for our related education and public outreach activity, 'Interaction of Magnetospheric Particles Applied to Classroom Teaching (IMPACT).'

Cooper, John F.↗